CN109907161A - A kind of biological fermentation feed and preparation method thereof - Google Patents
A kind of biological fermentation feed and preparation method thereof Download PDFInfo
- Publication number
- CN109907161A CN109907161A CN201910359217.4A CN201910359217A CN109907161A CN 109907161 A CN109907161 A CN 109907161A CN 201910359217 A CN201910359217 A CN 201910359217A CN 109907161 A CN109907161 A CN 109907161A
- Authority
- CN
- China
- Prior art keywords
- biological fermentation
- parts
- preparation
- fermentation feed
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of biological fermentation feed and preparation method thereof, the biological fermentation feed includes the raw material of following parts by weight: 15-45 parts of citrus pulp, 10-40 parts of sweet potato waste, 10-30 parts of pomace, it 5-15 parts of wheat bran, 5-15 parts of cottonseed cake, ferments through microbial composite bacteria kind；Biological fermentation feed raw material provided by the invention is made of agricultural by products such as citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cakes, can significantly reduce feed cost；And biological fermentation feed has thick protein, the free amino acid of high level, reduces the anti-nutritional factors content such as crude fibre and gossypol in raw material, improves the palatability and nutritive value of feedstuff；The grain feeds resources such as alternative or part Substitution for Soybean Meal, corn are conducive to alleviate China's feed resource status in short supply.
Description
Technical field
The present invention relates to biological feedstuff technical fields more particularly to a kind of biological fermentation feed and preparation method thereof.
Background technique
In the case where China arable land and water resource are long-term in short supply, with the fast development of animal husbandry, people and animals are total to grain
Contradiction it is increasingly prominent, feed resource shortage, it is at high cost the problems such as restrict the development of China's animal husbandry for a long time.Therefore, it develops
Novel fodder resource, production environmental health type feed are of great significance to promotion sustainable development of animal husbandry.
Existing feed mostly uses greatly conventional feed raw material to be fed, such as: corn, the grain resources such as dregs of beans.With
The development of novel fodder development of resources technology, developed countries, especially European countries gradually apply biofermentation at the beginning
Feed.And domestic biological fermentation feed technology at present falls behind relatively, and more, the application of other byproducts is only studied in terms of vinasse
Seldom, and the purpose of domestic biological fermentation feed fermentation is mainly to improve the palatability of former feed, the raising of nutritive value
It is not obvious, especially the content of protein and amino acid.Therefore, with citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake etc.
Agricultural by product is raw material, develops the biological fermentation feed of high nutritive value, to grains such as substitution or part Substitution for Soybean Meal, corns
It eats feed resource and reduces feed cost, be of great significance and development prospect.
Summary of the invention
In view of this, the present invention provides a kind of biological fermentation feed and preparation method thereof, this biological fermentation feed raw material
It is made of agricultural by products such as citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cakes, feed cost can be significantly reduced, and after fermentation
Biological fermentation feed palatability number, nutritive value is high.
A kind of biological fermentation feed, the raw material including following parts by weight:
Preferably, the raw material including following parts by weight:
Preferably, the raw material including following parts by weight:
A kind of preparation method of biological fermentation feed, comprising the following steps:
S1. it by citrus pulp, sweet potato waste, pomace, wheat bran and cottonseed cake impurity elimination, crushed mesh, water be added and is made into liquid
Raw material culture medium；
S2. the composite bacteria seed liquor after activation is inoculated in liquid charging stock culture medium by 5%-30% inoculum concentration,
Ferment 2-6d under 24 DEG C -36 DEG C of constant temperature, obtains fermented feed.
Wherein, the S1 step specifically: by citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake remove soil, stone,
The impurity such as branch crushed 40 meshes, mix in proportion, and sterile water is added, and obtain the liquid that water content is 40%-60%
Raw material culture medium；
The S2 step specifically: the composite bacteria after activation is inoculated in liquid charging stock training by 20%-30% inoculum concentration
It supports in base, ferment 4-5d under 28 DEG C -30 DEG C of constant temperature, obtains fermented feed.
Wherein, the composite bacteria is by lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili group
At.
Preferably, the lactobacillus fermenti of the composite bacteria, bacillus subtilis, saccharomyces cerevisiae and candida utili
Ratio be 1:1:1:1.
Wherein, further include actication of culture and the preparation of strain seed liquor between the S1 step and S2 step, specifically include:
Actication of culture: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and candida utili point
It is not inoculated on slant medium, stands constant temperature incubation, the strain after being activated；
The preparation of strain seed liquor: the strain after activation is seeded in fluid nutrient medium with oese respectively, is shaken in constant temperature
It is cultivated in bed, obtains strain seed liquor.
Wherein, the actication of culture specifically: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae
It is inoculated into respectively with candida utili through 121 DEG C of temperature sterilizing 30min, by 1% peptone, 1% yeast powder, 2% glucose
And 2% agar composition slant medium on, stand 30 DEG C of constant temperature, cultivate 48h, the strain after being activated；
The strain seed liquor preparation specifically: be inoculated into the strain after activation through 121 DEG C of temperature with oese respectively
Sterilize 30min, by 1% peptone, 1% yeast powder and 2% glucose group at fluid nutrient medium in, on 30 DEG C of constant-temperature tables
Culture, revolving speed 150r/min；Culture obtains strain seed liquor for 24 hours.
The beneficial effects of the invention are that: provide a kind of biological fermentation feed and preparation method thereof, life provided by the invention
Object fermented feed raw material is made of agricultural by products such as citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cakes, can significantly reduce feed
Cost；And biological fermentation feed has thick protein, the free amino acid of high level, reduces crude fibre and cotton in raw material
The anti-nutritional factors content such as phenol improves the palatability and nutritive value of feedstuff；Alternative or part Substitution for Soybean Meal, jade
The grain feeds resources such as rice are conducive to alleviate China's feed resource status in short supply.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are some of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The scope of protection of the invention.
It should be appreciated that ought use in this specification and in the appended claims, term " includes " and "comprising" instruction
Described feature, entirety, step, operation, the presence of element and/or component, but one or more of the other feature, whole is not precluded
Body, step, operation, the presence or addition of element, component and/or its set.
It will be further appreciated that the term "and/or" used in description of the invention and the appended claims is
Refer to any combination and all possible combinations of one or more of associated item listed, and including these combinations.
Influence of the 1 fermentation raw material culture medium prescription of embodiment to biological fermentation feed ferment effect
Raw material including following parts by weight:
First group: 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, 15 parts of cottonseed cake；
Second group: 45 parts of citrus pulp, 10 parts of sweet potato waste, 30 parts of pomace, 5 parts of wheat bran, 10 parts of cottonseed cake；
Third group: 15 parts of citrus pulp, 40 parts of sweet potato waste, 30 parts of pomace, 10 parts of wheat bran, 5 parts of cottonseed cake；
4th group: 40 parts of citrus pulp, 20 parts of sweet potato waste, 10 parts of pomace, 15 parts of wheat bran, 15 parts of cottonseed cake.
Above-mentioned four groups of material components are prepared by the following method respectively:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, be then uniformly mixed respectively by different proportion, sterile clear water is added, adjustment material-water ratio is
1:1 (i.e. water content 50%) obtains liquid charging stock culture medium, spare.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, cultivated on 30 DEG C of constant-temperature tables,
Revolving speed 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili strain seed liquor are pressed
Inoculative proportion is 1:1:1:1, and the ratio that total inoculum concentration is 20% is inoculated into the liquid charging stock culture medium prepared, at 30 DEG C
Ferment 4d under constant temperature, and biological fermentation feed can be obtained.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluation hair
Influence of the ferment raw material culture medium prescription to ferment effect；The biological fermentation feed product of different fermentations raw material culture medium prescription preparation
Gross protein value comparative determination result and calculating data are as follows:
As seen from the above table, using citrus pulp, sweet potato waste, pomace, wheat bran and cottonseed cake as fermentation substrate material, by biofermentation
Afterwards, the biological fermentation feed gross protein value obtained has all obtained apparent growth.Wherein when fermentation raw material culture medium prescription
When for 45 parts of citrus pulp, 10 parts of sweet potato waste, 30 parts of pomace, 5 parts of wheat bran, 10 parts of cottonseed cake, the thick egg of obtained biological fermentation feed
White mass content increase rate is maximum, reaches 58.87%；And when fermentation raw material culture medium prescription is 25 parts of citrus pulp, sweet potato waste 30
When part, 15 parts of pomace, 15 parts of wheat bran, 15 parts of cottonseed cake, obtained biological fermentation feed crude protein mass content is up to
23.37%, statistics indicate that, after biofermentation, feedstuff nutritive value is obviously improved.Therefore, comprehensively consider
From the point of view of feedstuff cost and ferment effect, with 45 parts of citrus pulp, 10 parts of sweet potato waste, 30 parts of pomace, 5 parts of wheat bran, cottonseed cake
10 parts are preferred fermentation raw material culture medium prescription.But with first group in subsequent embodiment, i.e. 25 parts of citrus pulp, sweet potato waste 30
Part, 15 parts of pomace, 15 parts of wheat bran, 15 parts of cottonseed cake studied for fermentation raw material culture medium prescription.
Influence of the 2 microorganism fungus kind inoculative proportion of embodiment to biological fermentation feed ferment effect
Control group is not inoculated with, and test group lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili connect
Kind ratio is respectively as follows: 1:1:1:1,1:2:1:1,1:1:2:1,1:1:1:2, is prepared by the following method respectively:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, then with 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, cottonseed cake
15 parts of ratio is uniformly mixed, and sterile clear water is added, and adjustment material-water ratio is 1:1 (i.e. water content 50%), obtains liquid charging stock training
Base is supported, it is spare.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, in 30 DEG C of constant-temperature table cultures, turn
Fast 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili strain seed liquor are pressed
Different vaccination ratio, the ratio that total inoculum concentration is 20% is inoculated into the liquid charging stock culture medium prepared, in 30 DEG C of constant temperature
Under the conditions of ferment 4d, biological fermentation feed can be obtained.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluates bacterium
Influence of the kind different vaccination ratio to ferment effect, the biological fermentation feed product thick protein of strain different vaccination ratio preparation
Content balance the result is as follows:
As seen from the above table, the biological fermentation feed gross protein value that strain inoculative proportion difference obtains has certain difference
It is different, but can significantly improve the gross protein value of product；Wherein, when lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and
The inoculative proportion of candida utili is 1:1:1:1, and the gross protein value highest of biological fermentation feed product can achieve
23.4%.
Influence of 3 inoculum concentration of embodiment to biological fermentation feed ferment effect
Control group is not inoculated with, and test group presses lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili
Inoculative proportion 1:1:1:1 is prepared by the following method respectively in different vaccination amount:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, then with 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, cottonseed cake
15 parts of ratio is uniformly mixed, and sterile clear water is added, and adjustment material-water ratio is 1:1 (i.e. water content 50%), obtains liquid charging stock training
Base is supported, it is spare.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, in 30 DEG C of constant-temperature table cultures, turn
Fast 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili are pressed into strain seed liquor
Inoculative proportion 1:1:1:1, different vaccination amount are inoculated into the liquid charging stock culture medium prepared, are issued in 30 DEG C of constant temperatures
Ferment 4d, can be obtained biological fermentation feed.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluation is not
Influence with inoculum concentration to ferment effect；The biological fermentation feed product gross protein value comparing result of different vaccination amount preparation
It is as follows:
Influence of the inoculum concentration to biological fermentation feed product gross protein value is significantly, when inoculum concentration is in 20%-
When between 30%, biological fermentation feed product gross protein value highest is thick in biological fermentation feed when inoculum concentration is 25%
The content highest of protein, can reach 22.47%.
Influence of 4 moisture content of embodiment to biological fermentation feed ferment effect
When configuring liquid charging stock culture medium, be respectively adopted water content be 40%, 44%, 48%, 52%, 56% and
60%；It is prepared by the following method:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, then with 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, cottonseed cake
15 parts of ratio is uniformly mixed, and sterile clear water is added, adjustment obtains different material-water ratio examples, obtains liquid charging stock culture medium, standby
With.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, in 30 DEG C of constant-temperature table cultures, turn
Fast 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili strain seed liquor are pressed
Inoculative proportion 1:1:1:1, total inoculum concentration is inoculated into the liquid charging stock culture medium prepared for 25%, in 30 DEG C of constant temperatures
Lower fermentation 4d, can be obtained biological fermentation feed.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluation is not
Influence with moisture content to ferment effect, the biological fermentation feed product gross protein value comparison of different in moisture content preparation
As a result as follows:
As seen from the above table, influence of the moisture content to biological fermentation feed product gross protein value is non-in raw material culture medium
Chang Xianzhu.When water content is lower than 52%, with the increase of moisture content, the gross protein value of product increases sharply；But work as
When moisture content is higher than 52%, with the increase of moisture content, the gross protein value of product declines instead.Wherein moisture content
In 48%-52%, gross protein value is higher in biological fermentation feed product.
Influence of 5 fermentation time of embodiment to biological fermentation feed ferment effect
When fermenting to liquid charging stock culture medium, fermentation time is respectively 2,3,4,5 and 6d, is made by the following method
It is standby:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, then with 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, cottonseed cake
15 parts of ratio is uniformly mixed, and sterile clear water is added, and adjustment water content is 52%, obtains liquid charging stock culture medium, spare.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, in 30 DEG C of constant-temperature table cultures, turn
Fast 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili strain seed liquor are pressed
Inoculative proportion 1:1:1:1, total inoculum concentration is inoculated into the liquid charging stock culture medium prepared for 25%, in 30 DEG C of constant temperatures
Lower fermentation 2-6d, can be obtained biological fermentation feed.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluation is not
Influence with fermentation time to ferment effect, the biological fermentation feed product gross protein value comparison of different fermentations time preparation
As a result as follows:
As seen from the above table, influence of the fermentation time to the gross protein value of biological fermentation feed product is more significant.It is raw
The gross protein value of object fermented feed product reached peak at the 5th day, wherein gross protein value in fermentation 4-6d product
It is higher.
Influence of 6 fermentation temperature of embodiment to biological fermentation feed ferment effect
When fermenting to liquid charging stock culture medium, fermentation temperature is controlled respectively for 24 DEG C, 26 DEG C, 28 DEG C, 30 DEG C, 32
DEG C, 34 DEG C and 36 DEG C, prepared by the following method:
Pretreatment of raw material and mixing: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake are simply removed into soil, stone, tree
After the impurity such as branch, it crushed 40 meshes, then with 25 parts of citrus pulp, 30 parts of sweet potato waste, 15 parts of pomace, 15 parts of wheat bran, cottonseed cake
15 parts of ratio is uniformly mixed, and sterile clear water is added, and adjustment water content is 52%, obtains liquid charging stock culture medium, spare.
The activation of slant strains: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast be inoculated into respectively containing peptone 1%, yeast powder 1%, glucose 2%, agar 2% (pH naturally, 121 DEG C sterilizing
On slant medium 30min), 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated.
Strain seed liquor preparation: by the strain after activation respectively with oese be seeded to containing contain peptone 1%, yeast
Powder 1%, glucose 2% (pH naturally, 121 DEG C sterilizing 30min) fluid nutrient medium in, in 30 DEG C of constant-temperature table cultures, turn
Fast 150r/min；Culture obtains strain seed liquor for 24 hours.
Liquid fermentation: lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili are pressed into inoculative proportion 1:
1:1:1, total inoculum concentration are inoculated into the liquid charging stock culture medium prepared for 25%, and ferment 5d under different constant temperatures,
Biological fermentation feed can be obtained.
Biological fermentation feed is dried at 85 DEG C, with the gross protein value of Kjeldahl nitrogen determination product, evaluation is not
Influence with fermentation temperature to ferment effect, the biological fermentation feed product gross protein value comparison of different fermentations temperature preparation
As a result as follows:
As seen from the above table, influence of the fermentation temperature to the gross protein value of biological fermentation feed product is highly significant
's.When fermentation temperature is less than 28 DEG C, the gross protein value of biological fermentation feed product is quicklyd increase with the raising of temperature；
When temperature is higher than 30 DEG C, the gross protein value of biological fermentation feed product is drastically reduced with temperature raising.Therefore, more suitable
The fermentation temperature of conjunction is 28 DEG C -30 DEG C, and when fermentation temperature is at 30 DEG C, the gross protein value in biological fermentation feed product can
To reach 23.96%.
The analysis of 7 biological fermentation feed nutritional components of the product of embodiment
Fermentation temperature is the biological fermentation feed product being prepared at 30 DEG C in selection example 6, is surveyed after 85 DEG C of drying
The content of its fixed thick protein, crude fibre, crude fat, coarse ash, moisture, gossypol, free amino acid, as a result see the table below.
As seen from the above table, after the raw materials such as citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake undergo microbial fermentation, product
Middle gross protein value increases to 23.79% from 17.74%, and crude fiber content is reduced to 9.55% by 13.61%.And microorganism
Fermentation has apparent degradation to gossypol, and gossypol content is reduced to 0.015% (it is permitted to be lower than national standard by 0.16%
Gossypol content≤0.02%), degradation rate has reached 90.62%.
In addition, 16 kinds of amino acid contents have different degrees of raising in biological fermentation feed product, wherein aspartic acid
Content raising is the most obvious, reaches 391%, followed by valine, methionine, threonine, serine and leucine, increase rate is equal
It has been more than 100%.
To sum up, the present invention is led to using agricultural by products such as citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cakes as fermentation raw material
Complex microorganism bacterial classification fermentation is crossed, thick protein in feedstuff, free aminoacid content is significantly improved, reduces crude fibre
And the anti-nutritional factors content such as gossypol, improve the palatability and nutritive value of feedstuff.
The steps in the embodiment of the present invention can be sequentially adjusted, merged and deleted according to actual needs.
It is described in detail herein, applies specific case and the principle of the present invention and embodiment are explained
It states, above embodiments are merely used to help understand method and its core concept of the invention；Meanwhile for the general skill of this field
Art personnel, according to the thought of the present invention, there will be changes in the specific implementation manner and application range, in conclusion this
Description should not be construed as limiting the invention.
It is apparent to those skilled in the art that for convenience and simplicity of description, the system of foregoing description
It with the specific work process of unit, can refer to corresponding processes in the foregoing method embodiment, details are not described herein.
Claims (10)
1. a kind of biological fermentation feed, which is characterized in that the raw material including following parts by weight:
2. biological fermentation feed according to claim 1, which is characterized in that the raw material including following parts by weight:
3. biological fermentation feed according to claim 1 to 2, which is characterized in that the original including following parts by weight
Material:
4. a kind of preparation method of biological fermentation feed, which comprises the following steps:
S1. it by citrus pulp, sweet potato waste, pomace, wheat bran and cottonseed cake impurity elimination, crushed mesh, water be added and is made into liquid charging stock
Culture medium；
S2. the composite bacteria seed liquor after activation is inoculated in liquid charging stock culture medium by 5%-30% inoculum concentration, 24 DEG C-
Ferment 2-6d under 36 DEG C of constant temperature, obtains biological fermentation feed.
5. the preparation method according to claim 4, which is characterized in that
The S1 step specifically: citrus pulp, sweet potato waste, pomace, wheat bran, cottonseed cake removing soil, stone, branch etc. is miscellaneous
Matter crushed 40 meshes, mix in proportion, and sterile water is added, and obtain the liquid charging stock culture that water content is 40%-60%
Base.
6. the preparation method according to claim 4, which is characterized in that
The S2 step specifically: the composite bacteria after activation is inoculated in liquid charging stock culture medium by 20%-30% inoculum concentration
In, ferment 4-5d under 28 DEG C -30 DEG C of constant temperature, obtains biological fermentation feed.
7. according to any preparation method of claim 4-6, which is characterized in that
The composite bacteria is made of lactobacillus fermenti, bacillus subtilis, saccharomyces cerevisiae and candida utili.
8. preparation method according to claim 7, which is characterized in that
The lactobacillus fermenti of the composite bacteria, bacillus subtilis, saccharomyces cerevisiae and candida utili ratio be 1:
1:1:1。
9. the preparation method according to claim 4, which is characterized in that
Further include actication of culture and the preparation of strain seed liquor between the S1 step and S2 step, specifically include:
Actication of culture: the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and candida utili are connect respectively
In kind to slant medium, constant temperature incubation, the strain after being activated are stood；
The preparation of strain seed liquor: the strain after activation is seeded in fluid nutrient medium with oese respectively, in constant-temperature table
Culture, obtains strain seed liquor.
10. preparation method according to claim 9, which is characterized in that
The actication of culture specifically: by the lactobacillus fermenti of freezing, bacillus subtilis, saccharomyces cerevisiae and Candida utilis
Yeast is inoculated into respectively through 121 DEG C of temperature sterilizing 30min, by 1% peptone, 1% yeast powder, 2% glucose and 2% agar
On the slant medium of composition, 30 DEG C of constant temperature are stood, cultivates 48h, the strain after being activated；
The strain seed liquor preparation specifically: the strain after activation is inoculated into oese respectively and is sterilized through 121 DEG C of temperature
30min, by 1% peptone, 1% yeast powder and 2% glucose group at fluid nutrient medium in, trained on 30 DEG C of constant-temperature tables
It supports, revolving speed 150r/min；Culture obtains strain seed liquor for 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910359217.4A CN109907161A (en) | 2019-04-30 | 2019-04-30 | A kind of biological fermentation feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910359217.4A CN109907161A (en) | 2019-04-30 | 2019-04-30 | A kind of biological fermentation feed and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109907161A true CN109907161A (en) | 2019-06-21 |
Family
ID=66978923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910359217.4A Pending CN109907161A (en) | 2019-04-30 | 2019-04-30 | A kind of biological fermentation feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109907161A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631306A (en) * | 2020-06-16 | 2020-09-08 | 江西省科学院生物资源研究所 | Application of broussonetia papyrifera fermentation material and yeast culture byproducts in broussonetia papyrifera fermentation |
| CN112021451A (en) * | 2020-08-07 | 2020-12-04 | 东北农业大学 | Fermentation method for increasing content of fatty and acid soluble proteins in wheat bran |
| CN112868888A (en) * | 2021-02-03 | 2021-06-01 | 广东省农业科学院动物科学研究所 | Biological fermentation preparation and preparation method thereof |
| CN113875887A (en) * | 2021-09-16 | 2022-01-04 | 广东省农业科学院动物科学研究所 | Citrus residue-based fermentation preparation and preparation method and application thereof |
| CN114468127A (en) * | 2022-03-21 | 2022-05-13 | 贵州大学 | Method for fermenting sweet potato residues by using probiotics and application thereof |
| CN115024404A (en) * | 2022-05-24 | 2022-09-09 | 贵州省畜牧兽医研究所 | Feed for Guizhou yellow cattle and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972655A (en) * | 2012-11-30 | 2013-03-20 | 来光业 | Preparation method of microbial fermented feed without antibiotic for sow |
| CN103392939A (en) * | 2013-08-05 | 2013-11-20 | 山东省农业科学院畜牧兽医研究所 | Fermented feed for sows |
| CN106721028A (en) * | 2017-01-12 | 2017-05-31 | 江苏富海生物科技有限公司 | A kind of milk cow fermented feed and preparation method thereof |
| CN106721261A (en) * | 2016-11-30 | 2017-05-31 | 辽宁禾丰牧业股份有限公司 | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof |
| CN106889318A (en) * | 2015-12-17 | 2017-06-27 | 熊立寅 | The mixed mixed feed of the special thick, flour and rice of one kind production sow |
| CN107821817A (en) * | 2017-11-18 | 2018-03-23 | 林州亚太兴牧科技有限公司 | One broad sow biological fermentation feed and preparation method thereof |
| CN108185187A (en) * | 2018-02-14 | 2018-06-22 | 广东大广生物科技有限公司 | The sweet tea napier grass fermentation material and preparation method of raising farrowing sow production performance and application |
| CN108617876A (en) * | 2018-07-13 | 2018-10-09 | 黄梅县强立畜牧有限公司 | A kind of feed and preparation method thereof improving reproduction performance of gestation sow |
| CN109221710A (en) * | 2018-09-29 | 2019-01-18 | 河南牧业经济学院 | A kind of pregnant sow liquid state fermentation complete feed and preparation method thereof |
-
2019
- 2019-04-30 CN CN201910359217.4A patent/CN109907161A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972655A (en) * | 2012-11-30 | 2013-03-20 | 来光业 | Preparation method of microbial fermented feed without antibiotic for sow |
| CN103392939A (en) * | 2013-08-05 | 2013-11-20 | 山东省农业科学院畜牧兽医研究所 | Fermented feed for sows |
| CN106889318A (en) * | 2015-12-17 | 2017-06-27 | 熊立寅 | The mixed mixed feed of the special thick, flour and rice of one kind production sow |
| CN106721261A (en) * | 2016-11-30 | 2017-05-31 | 辽宁禾丰牧业股份有限公司 | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof |
| CN106721028A (en) * | 2017-01-12 | 2017-05-31 | 江苏富海生物科技有限公司 | A kind of milk cow fermented feed and preparation method thereof |
| CN107821817A (en) * | 2017-11-18 | 2018-03-23 | 林州亚太兴牧科技有限公司 | One broad sow biological fermentation feed and preparation method thereof |
| CN108185187A (en) * | 2018-02-14 | 2018-06-22 | 广东大广生物科技有限公司 | The sweet tea napier grass fermentation material and preparation method of raising farrowing sow production performance and application |
| CN108617876A (en) * | 2018-07-13 | 2018-10-09 | 黄梅县强立畜牧有限公司 | A kind of feed and preparation method thereof improving reproduction performance of gestation sow |
| CN109221710A (en) * | 2018-09-29 | 2019-01-18 | 河南牧业经济学院 | A kind of pregnant sow liquid state fermentation complete feed and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 石丽: "微生物发酵饲料在母猪中的应用", 《农业知识》 * |
| 蒋爱国: "果渣发酵饲料制作技术", 《农村新技术》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631306A (en) * | 2020-06-16 | 2020-09-08 | 江西省科学院生物资源研究所 | Application of broussonetia papyrifera fermentation material and yeast culture byproducts in broussonetia papyrifera fermentation |
| CN111631306B (en) * | 2020-06-16 | 2023-09-15 | 江西省科学院生物资源研究所 | Application of broussonetia papyrifera fermentation material and yeast culture byproducts in broussonetia papyrifera fermentation |
| CN112021451A (en) * | 2020-08-07 | 2020-12-04 | 东北农业大学 | Fermentation method for increasing content of fatty and acid soluble proteins in wheat bran |
| CN112868888A (en) * | 2021-02-03 | 2021-06-01 | 广东省农业科学院动物科学研究所 | Biological fermentation preparation and preparation method thereof |
| CN112868888B (en) * | 2021-02-03 | 2021-08-17 | 广东省农业科学院动物科学研究所 | Biological fermentation preparation and preparation method thereof |
| CN113875887A (en) * | 2021-09-16 | 2022-01-04 | 广东省农业科学院动物科学研究所 | Citrus residue-based fermentation preparation and preparation method and application thereof |
| CN114468127A (en) * | 2022-03-21 | 2022-05-13 | 贵州大学 | Method for fermenting sweet potato residues by using probiotics and application thereof |
| CN115024404A (en) * | 2022-05-24 | 2022-09-09 | 贵州省畜牧兽医研究所 | Feed for Guizhou yellow cattle and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109907161A (en) | A kind of biological fermentation feed and preparation method thereof | |
| CN106222114B (en) | The bacillus of efficient degradation dregs of beans antigen protein and the method for bacterium enzyme mixed fermentation | |
| CN101912040B (en) | Tomato pomace biological fermentation feed and preparation method thereof | |
| CN1899079B (en) | Method for preparing protein feed by solid fermenting potato slag | |
| CN110037165A (en) | A kind of Heisui River gadfly slurry solid state fermentation concentrated feed and preparation method | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| CN103396956B (en) | A kind of saccharomyces cerevisiae and its screening and culturing method and the method for bean pulp fermentation | |
| CN102599351A (en) | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof | |
| CN103820339B (en) | A kind of dehydrated solid-state combination microbial inoculum improving manioc waste protein level and preparation method thereof | |
| CN101692869B (en) | Method for producing high protein bean pulp based on two-step microbial fermentation | |
| CN102669483A (en) | Mixed plant protein fish feed as well as preparation method and application thereof | |
| CN102113629B (en) | Method for preparing dairy cow feed by microbial fermentation | |
| CN103156051B (en) | Method using composite bacterium to ferment bean dregs to manufacture protein feed | |
| CN106615658A (en) | Method for preparing fermentation bed padding by recycling froggrass | |
| CN107535671A (en) | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate | |
| CN109757603A (en) | A kind of method that fermentation soybean grouts prepare pannage | |
| CN105211509A (en) | A kind ofly utilize feed of pomelo peel ferment making and preparation method thereof | |
| CN104855674A (en) | Production method for microbial fermentation complete feed by combining strain joint transformations | |
| CN103275875A (en) | Trichoderma koningii, and compound microbial agent composition and application thereof | |
| CN109423466A (en) | A kind of composite fermentation microbial inoculum and its application | |
| CN102138632A (en) | Method for removing free gossypol from cotton dregs by biological fermentation method | |
| CN1248592C (en) | Dehusk and detoxin of cotton-seed cake to produce protein feed | |
| CN105901282A (en) | A method of preparing animal protein feed from potato dregs through fermentation and the feed prepared by the method | |
| CN103156091A (en) | Method for manufacturing biologically-fermented straw nutrition forage grass at low temperature for beef | |
| CN103168914B (en) | Preparation technology of microbial glycolysis active peptide containing haematochrome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190621 |