CN102755505B - Traditional Chinese medicinal herb mixture for preventing and treating new castle disease and production technology - Google Patents
Traditional Chinese medicinal herb mixture for preventing and treating new castle disease and production technology Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal herb mixture for preventing and treating new castle disease, and belongs to the technical field of veterinary medicaments. The Chinese medicinal herb mixture is prepared from the following raw medicaments: honeysuckle, folium isatidis, isatis root, Baikal skullcap root, weeping forsythiae, gypsum, anemarrhenae, jasmine, platycodon grandiflorum, apricot kernel, fritillary bulb and liquorice. The Chinese medicinal herb mixture has a strong inhibiting effect on the new castle disease, has a good prevention and control effect, and does not have toxic or side effect.
Description
Technical field
The present invention relates to a kind of medicine of preventing and treating chicken viral diseases, specifically a kind of herbal mixture and production technology of preventing and treating newcastle disease.
Background technology
Viral infectious is the disease that the serious harm poultry husbandry develops in a healthy way, and there is no at present the specific treatment medicine, though there is specific antibody to be treated, due to problems such as cross infections, clinical being difficult to used on a large scale and promote.The characteristics such as Chinese medicine has aspect chicken viral diseases with the disease plus-minus in prevention and treatment, compatibility, treating both the principal and secondary aspects of a disease, low toxicity be efficient flexibly are the choice drugs of prevention and treatment chicken viral diseases; Be to reduce drug residue, improve the poultry product quality, guarantee the most effective technical measures of human health.
Newcastle disease (Newcastle disease, ND), be a kind of height contagious disease caused by paramyxovirus, claims again philippine fowl disease or pseudo-fowl plague.Often be the acute sepsis shape, mortality rate is high, serious to poultry husbandry harm.Principal character is dyspnea, just rare, neurological disorders, mucosa and serous coat are hemorrhage.At first nineteen twenty-six is found in Indonesia, at Britain's new city, finds again soon, and all there is popular record countries in the world.Virulent strain and low virulent strain two classes are arranged.Virus is divided into low virulence type (being delayed type), mesogenic type (hair style), strong virus force type (being anaphylactic type) 3 types.Most high strength virulent strains are had a liking for the visceroatonia type Avian pneumo-encephalitis virus normal the genus.The equal susceptible of chicken, turkey and pheasant, the susceptible of family chicken, chickling is higher than Adult Chicken susceptibility.The image thoughts are caught conjunctivitis.The acute disease is fervescence just, can reach 44 ℃, down in spirits, and the feather pine disorderly, is the lethargy shape.Hat and wattle kermesinus or black purple.Crop interior liquid and the gas of often being full of of speaking in a low voice, dyspnea, throat sends snore; Feces is thin, stench, death in general 2～5 days.Subacute or the chronic type symptom is similar to acute, the state of an illness is lighter, and nervous symptoms appears in the later stage, lower limb, wing paralysis, and movement disorder, head is to layback or, to crooked etc. on one side, the course of disease can reach 1～2 month, most final dead.There is no at present effective medicine, mainly rely on strict sterilization, isolation and vaccination to prevent.
Summary of the invention
The technical problem that invention will solve is to provide a kind of herbal mixture of preventing and treating newcastle disease, and it is evident in efficacy, has no side effect.
Technical scheme of the present invention is: a kind of herbal mixture of preventing and treating newcastle disease, it is characterized in that, made by the crude drug of following weight parts: Flos Lonicerae 8～15, Folium Isatidis 5～15, Radix Isatidis 10～15, Radix Scutellariae 5～10, Fructus Forsythiae 10～15, Gypsum Fibrosum 18～22, the Rhizoma Anemarrhenae 8～12, Fructus Gardeniae 8～12, Radix Platycodonis 5～15, Semen Armeniacae Amarum (stir-fry) 5～10, Bulbus Fritillariae Thunbergii 2～10, Radix Glycyrrhizae 3～5.
Optimum ratio is (weight portion): Flos Lonicerae 8, Folium Isatidis 8, Radix Isatidis 10, Radix Scutellariae 8, Fructus Forsythiae 10, Gypsum Fibrosum 19, the Rhizoma Anemarrhenae 10, Fructus Gardeniae 8, Radix Platycodonis 7, Semen Armeniacae Amarum (stir-fry) 5, Bulbus Fritillariae Thunbergii 3, Radix Glycyrrhizae 4.
Production technology is:
(1) soak: take by weight each crude drug standby; Flos Lonicerae, Folium Isatidis are soaked together with Radix Scutellariae; Gypsum Fibrosum is smashed single bag; Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii are soaked together with Radix Glycyrrhizae;
(2) decoct: Gypsum Fibrosum is decocted first 30～50 minutes, then adds Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii and Radix Glycyrrhizae to boil 30～50 minutes, adds Flos Lonicerae, Folium Isatidis, Radix Scutellariae to boil 30～50 minutes again, filters; Filtering residue adds water boil 1～1.5 hour, filters, and merges filtrate twice;
(3) post processing: after adding antiseptic after vacuum decompression (<60kpa) is concentrated, mixture is made in cooling fill.
The property of medicine and the pharmacology analysis of the selected Chinese herbal medicine of the present invention are as follows:
Flos Lonicerae: be caprifoliaceae plant dry flower or the first flower of opening of band of Radix Ophiopogonis.Sweet in the mouth, cold in nature.Return lung, the heart, stomach warp.The tool heat-clearing and toxic substances removing, the effect of dispelling wind and heat pathogens.Cure mainly the epidemic febrile disease heating, anemopyretic cold, cough due to lung-heat, laryngopharynx swelling and pain, toxic-heat and blood stasis, breast swell and pain, carbuncle furuncle.Modern medicine study confirms that Flos Lonicerae has the effects such as anti-multiple pathogenic microorganisms, antiinflammatory are analgesic, antiendotoxin, booster immunization function, stimulating central nervous system.
Folium Isatidis: be the dried leaves of cruciferae isatis.Bitter in the mouth, cold in nature.GUIXIN, stomach warp.The tool heat-clearing and toxic substances removing, the effect of removing heat from blood sore-throat relieving.Cure mainly anemopyretic cold, laryngopharynx swelling and pain, aphtha of the mouth and tongue, skin ulcer general edema with yellowish skin poison etc.Confirm that for medical research Folium Isatidis has the effects such as anti-multiple pathogenic microorganisms, endotoxin, antiinflammatory.
Radix Isatidis: be the dry root of cruciferae isatis.Bitter in the mouth, cold in nature.Return the liver heart, stomach warp.The tool heat clearing away, removing toxic substances, removing heat from blood, the effect of sore-throat relieving.Cure mainly maculae caused by violent heat pathogen, high heat headache, major part pestilence, crimson tongue is purple dark, scarlet fever, erysipelas, mumps, sore throat, skin ulcer is swollen, carbuncle, chickenpox, measles, hepatitis, influenza, epidemic encephalitis, encephalitis b, pneumonia, coma is told nosebleed, pharyngeal swelling, acute catarrhal conjunctivitis, the skin ulcer rash, can prevent and treat epidemic encephalitis type B, acute, chronic hepatitis, mumps, osteomyelitis.Modern medicine study confirms that Radix Isatidis has the effects such as anti-multiple pathogenic microorganisms, antiinflammatory, inhibition platelet aggregation.
Radix Scutellariae: be the dry root of labiate Radix Scutellariae.Bitter in the mouth, cold in nature.Return lung, gallbladder, spleen, large intestine, small intestine meridian.The tool heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive effect.Cure mainly the cough due to lung-heat, gastrointestinal is damp and hot, dysentery, and jaundice, the greedy drink of high heat, have blood in stool, epistaxis, conjunctival congestion and swelling pain, carbuncle sore tumefacting virus, the diseases such as frequent fetal movement.Modern medicine study confirm Radix Scutellariae tool resisting pathogenic microbes, antiinflammatory, adjusting immunologic function, analgesic, calm, protect the liver, the effect such as function of gallbladder promoting.
Fructus Forsythiae: be the dry fruit of Oleaceae plants Fructus Forsythiae.Bitter in the mouth, cold nature.Return lung, the heart, small intestine meridian.The tool heat-clearing and toxic substances removing, promoting the production of body fluid to quench thirst, the effect of dissipating blood stasis for subsidence of swelling.Cure mainly flu, heatstroke, laryngopharynx swelling and pain, high heat is thirsty, traumatic injury etc.Modern medicine study confirms that Fructus Forsythiae has anti-multiple pathogenic microorganisms, antiinflammatory, the effect such as protects the liver.
Gypsum Fibrosum: be Sulfates mineral anhydrite family Gypsum Fibrosum.Acrid in the mouth, sweet, property Great Cold.Attach to the lung and stomach meridians.The tool clearing away heat-fire, the effect of promoting the production of body fluid to quench thirst.Cure mainly fever caused by exogenous pathogenic factors, the lung-heat dyspnea with rapid and short breath is moved back, the greedy drink of gastric heat, high fever coma, manic uneasiness.Modern medicine study confirms that Gypsum Fibrosum has and brings down a fever, and spasmolytic, antiinflammatory, quenches one's thirst, and blood sugar lowering, replenish the calcium and sulfated polysaccharide turns use into, and enhancing immunity promotes blood coagulation, the effects such as function of gallbladder promoting and diuresis.
The Rhizoma Anemarrhenae: be the dry rhizome of the liliaceous plant Rhizoma Anemarrhenae.Bitter in the mouth, sweet, cold in nature.Return lung, stomach, kidney channel.The tool clearing away heat-fire, nourshing Yin and drynsessmoistening prescription effect.Cure mainly fever caused by exogenous pathogenic factors, gastric heat, the cough due to lung-heat, quench one's thirst, dryness of the intestine constipation, yin asthenia generating intrinsic heat.Modern medicine study confirms that the Rhizoma Anemarrhenae has remarkable resisting pathogenic microbes infection effect, refrigeration function, function of gallbladder promoting and diuresis.
Fructus Gardeniae: the dry mature fruit that is the Maguireothamnus speciosus Fructus Gardeniae.Bitter in the mouth, property Great Cold.GUIXIN, lung, tri-jiao channel.The tool eliminating fire and detoxication, clearing away heat and promoting diuresis, removing heat from blood, the effect of externally used detumescence pain relieving.Cure mainly the three warmers intenseness of heat, jaundice due to damp-heat, aphtha of the mouth and tongue, conjunctival congestion and swelling pain, heat in blood epistaxis, sudden sprain lumbago.Modern medicine study confirms that the tool Fructus Gardeniae has the effects such as hepatoprotective, function of gallbladder promoting, blood pressure lowering, calmness, hemostasis, detumescence.
Radix Platycodonis: be the dry rhizome of Campanulaceae Radix Platycodonis.Bitter in the mouth, suffering, slightly warm in nature.Return lung meridian.The tool lung qi dispersing, eliminate the phlegm, the effect of sore-throat relieving, evacuation of pus.Cure mainly cough with copious phlegm, laryngopharynx swelling and pain, lung abscess.Modern medicine study confirms that the Radix Platycodonis tool eliminates the phlegm, calmness, analgesia, analgesic and antiinflammatory action.
Semen Armeniacae Amarum: be the dry mature seed of rosaceous plant Prunus armeniaca L.var.ansu Maxim. (bitter Fructus Pruni), Siberia Fructus Pruni (Prunus armeniaca L.var.ansu Maxim.), prunus mandshuricaKoehne or Fructus Pruni.Bitter in the mouth, slightly warm in nature, slightly poisonous.Return lung, large intestine channel.The tool relieving cough and asthma, the effect of loosening bowel to relieve constipation.Cure mainly cough and asthma, the dryness of the intestine constipation.Modern medicine study confirms that Semen Armeniacae Amarum has antiinflammatory, analgesia, blood pressure lowering, the effect such as anticancer.
Bulbus Fritillariae Thunbergii: be the bulb of liliaceous plant Bulbus Fritillariae Thunbergii.Bitter in the mouth, cold in nature.Return lung, heart channel.Tool heat clearing away eliminating stagnation, the effect of preventing phlegm from forming and stopping coughing.Cure mainly the cough due to lung-heat, lung abscess, acute mastitis, sore swollen toxin.Modern medicine study confirms that Bulbus Fritillariae Thunbergii has antitussive, the effect such as eliminate the phlegm.
Radix Glycyrrhizae: be dry root and the rhizome of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L..Sweet in the mouth, property is flat.Enter the heart, lung, spleen, stomach warp.The tool invigorating the spleen and replenishing QI, expelling phlegm for arresting cough, and middle emergency, removing toxic substances, coordinating the actions of various ingredients in a prescription, cushion toxicity, strong.Cure mainly weakness of the spleen and stomach, fatigue and weakness, cough with asthma, laryngopharynx swelling and pain, poisoning, skin infection.Modern medicine study confirms that Radix Glycyrrhizae has antiinflammatory, antiviral, and the effects such as protecting liver and detoxication and enhancing immunologic function.
In the present invention, Flos Lonicerae, Folium Isatidis, Radix Isatidis, Fructus Forsythiae all have the effect of heat-clearing and toxic substances removing, cooling blood for hemostasis, are used for the treatment of body fear of cold heating, hemorrhage primary symptom, are principal agent altogether.Radix Scutellariae, Radix Platycodonis, Bulbus Fritillariae Thunbergii and Semen Armeniacae Amarum master enter lung meridian, and lung heat clearing is through excess-heat, the merit that the tool relieving cough and resolving phlegm is relievingd asthma, and the cardinal symptoms such as auxiliary principal agent treatment cough, asthma are accessory drugs altogether.Fructus Gardeniae enters tri-jiao channel, and clear three warmers are damp and hot; Gypsum Fibrosum, the Rhizoma Anemarrhenae have heat clearing away cooling, nourshing Yin and drynsessmoistening prescription, the function such as quench the thirst, and have both contributed to reduce body temperature, can restrict again the drawback of bitter dry consumption the moon of principal agent, and two medicines coordinate learns from other's strong points to offset one's weaknesses, and bring down a fever fast, effect by force and lastingly; Three medicines coordinate makes the principal agent heat-clearing toxin-expelling functions stronger, is adjuvant drug altogether.The panacea that Radix Platycodonis is lung man, both had a merit of depressed lung-energy dispersing, can enter lung meridian by priming again, clearing away lung-heat, resolving phlegm and relieving asthma, and Radix Glycyrrhizae had both played the effect of coordinating the actions of various ingredients in a prescription, had again a function of invigorating spleen for diuresis, and the two is altogether for making medicine.Above-mentioned 12 herbal medicine compatibility tool heat-clearing and toxic substances removing, cooling blood for hemostasis, cough-relieving, the effect of reducing phlegm, relievining asthma, cure mainly the cards such as unconsciousness due to high fever due to the heresy of poultry diseases caused by exogenous pathogenic factor pestilence, aversion to cold, cough with asthma tachypnea.
Newcastle disease is that the infectious disease of chicken infection Avian pneumo-encephalitis virus belongs to the pestilence category.At first attack appearance, fur when virus attack chicken body.Due to lung and fur, at first assorted hair, fear of cold appear, then occur that nasal cavity has mucus, the eyes symptom such as shed tears.Along with PD, the pathogenic heat going from the exterior into the interior, the thin nasal discharge tears that thicken, have mucus or sticky expectorant in trachea, impairment of purifying and descending function of the lung, abnormal rising of lung-QI and cough with asthma.Pathogenic heat is invaded taste, and loose stool material appears just and in dysfunction of spleen and stomach.Invade percardium as pathogenic heat, due to the heart controlling mental and emotional activities, coma will occur, drowsiness.As pathogenic heat invasion and attack liver, cause liver-wind stirring up internally, the nervous symptoms such as neck appear turning round.Because good and evil are fought mutually, pathogen preponderating over vital QI, bleeding due to blood-heat, spleen system blood functional disorder, blood oozing from the body openings or subcuta neous tissue is outside vascular, and a large amount of organ hemorrhages, as glandular stomach, trachea, larynx, subcutaneous, intestinal etc.Therefore, primary disease should be selected the medicine of heat-clearing and toxic substances removing, pathogenic fire purging, removes the main symptom of pathogenic heat in body, selects cooling blood for hemostasis, and relieving cough and resolving phlegm is relievingd asthma, and removes time card hemorrhage, cough with asthma, to reach the purpose for the treatment of both the principal and secondary aspects of a disease.
The invention has the beneficial effects as follows: newcastle disease virus is had to stronger inhibitory action, and prevention effect is good, has no side effect.
The specific embodiment
Embodiment 1
Proportioning (weight portion): Flos Lonicerae 8, Folium Isatidis 8, Radix Isatidis 10, Radix Scutellariae 8, Fructus Forsythiae 10, Gypsum Fibrosum 19, the Rhizoma Anemarrhenae 10, Fructus Gardeniae 8, Radix Platycodonis 7, Semen Armeniacae Amarum (stir-fry) 5, Bulbus Fritillariae Thunbergii 3, Radix Glycyrrhizae 4.
Production technology is:
(1) soak: take by weight each crude drug standby; Flos Lonicerae, Folium Isatidis, Radix Scutellariae are added to 6 times of amount warm water (40～50 ℃) to be soaked 1 hour; Gypsum Fibrosum is smashed single bag; By Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii, Radix Glycyrrhizae, add 6 times of amount warm water (40～50 ℃) and soak 1 hour.
(2) decoct: Gypsum Fibrosum is decocted first 30 minutes, then adds the 8 flavor Chinese medicines such as Radix Isatidis, Fructus Forsythiae (adding together with soaking water) to boil 30 minutes, adds Flos Lonicerae, Folium Isatidis and Radix Scutellariae (adding together with soaking water) to boil 30 minutes again, filters; Filtering residue adds water (add warm water, amount of water is 4 times of crude drug gross weight before not soaking) and boils for the second time 1 hour, filters, and merges filtrate twice.
(3) concentrated: vacuum decompression (<60kpa) is concentrated into 1g crude drug/mL.
(4) anticorrosion 0.5% sodium benzoate that adds is anticorrosion.
(5) when the liquid drug temperature drops to 50 ℃, fill while stirring, sealing.
Using method prevention consumption is that every day, 0.25～0.5g/kg body weight, drank water, and 2 times/days, is used in conjunction 3～5; Treatment, every day 1.0g～1.5g/kg body weight, drinking-water,, be used in conjunction 5～7 by 2 times/days.
Embodiment 1 Embryo Gallus domesticus anti-new castle disease virus experiment
1 test material and method
1.1 11 age in days SPF Embryo Gallus domesticus, provided by the Shandong safe Experimental Animal Center of sky.NDV-LaSota(EID
50be 10
-6.0/ 0.1mL), fowl diseases key lab in Shandong Province's provides.Amantadine hydrochloride, lot number 200602851, Dongbei Pharmaceutical General Factory production.PBS is made into 0.1% solution for standby.Moroxydine, pharmacy head factory in Liaoning is produced, lot number 200602049, PBS is made into 0.1% solution for standby.
1.2 Embryo Gallus domesticus is divided into to Chinese drug-treated group, western medicine group, normal saline matched group, virus control group at random, 15 pieces every group, continuous 4 repetitions.At first by Chinese medicine and equivalent 100EID
50virus liquid mixed at room temperature effect 10～60min, by 1% sodium thiosulfate cessation reaction, the inoculation of 0.2mL/ embryo allantoic cavity, carry out the time gradient test; Secondly respectively NDV and Chinese medicine are done to 10 times of serial dilutions, 10
-1-10
-6seven dilution factors, with equivalent Chinese medicine (or control drug) and NDV (10
-4) mix, room temperature effect 15min, paraffin sealing, hatch to 168h for 37 ℃ and carry out the Concentraton gradient test.Observe the Embryo Gallus domesticus pathological changes, measure chick embryo allantoic liquid NDV-HA and tire, add up its diversity.
2 result of the tests
2.1 it is in 10min that time gradient test TCM in Vitro is killed the effective acting time of 100EID50NDV, it is 0 that NDV-HA tires, specifically in Table 1.
The impact that table 1 Chinese medicine and viral immixture time tire on viral HA
2.2 Concentraton gradient test
2.2.1 Chinese medicine to the protective rate of variable concentrations virus inoculation Embryo Gallus domesticus to 1000EID
50the protective rate of NDV be 60.0%, to 100EID
50the protective rate of NDV be 100%, specifically in Table 2.
Table 2 impact that fixedly the Chinese medicine virus dilution is tired on viral HA
2.2.2 variable concentrations Chinese medicine is to 100EID
50the protective rate medicine stock solution of virus inoculation Embryo Gallus domesticus is to 100EID
50the protective rate of NDV be 100%, if 100 times of stock solution dilutions, to still effectively suppressing the activity of NDV, specifically in Table 3.
The impact that table 3 fixed virus dilution Chinese medicine virus HA tires
The impact of embodiment 2 Chinese medicines on the motion of chicken tracheal cilia
1, material SPF cock: 40 ages in days, average weight 290g; Medicine and reagent: urethanes, Chinese ink, cold light pocket lamp, slide gauge, mosquito forceps, shears, sterilized absorbent cotton etc.
2, method is divided into Chinese drug-treated group of the present invention and normal saline group at random by 20 cocks, 10 every group.Chinese drug-treated group perfusion 0.3mL/ time, 2 times/days, be used in conjunction 3d, the normal saline group gavages the equivalent normal saline.4d stops expecting 8h, with 20% urethane ester solution 3000mg/kg body weight intraperitoneal injection of anesthesia.After fiber crops, degradation is fixed on special platform extremely deeply, fixes and the stretching cervical region, and skin of neck, the sterilization of quilt hair, cut off cervical region by hair, with operating room, cuts off skin of neck, and the mosquito forceps hemostasis, fully expose, peel off trachea.Enter inner surface of trachea with the 1mL Bacillus Calmette-Guérin syringe from nearly heart terminal spine and push about 0.02mL Chinese ink, under dark indoor torch pocket lamp irradiates with vernier caliper measurement 1min in the distance that moves of prepared Chinese ink.
3, result
The impact of table 4 Chinese medicine on the motion of chicken tracheal cilia
Remarks: compare with the normal saline group
*p<0.01.
As can be seen from Table 3, Chinese medicine can extremely significantly promote the motion of tracheal cilia.
Embodiment 3 Immune Profile In Chicks are regulated experiment
1 materials and methods
Select 21 age nonimmune cock, do contrast with left-handed rice azoles, 3d is by 0.5% drinking-water in advance, the indexs such as the dynamic change of tiring by detection newcastle low virulence live vaccine (Lasota strain) immune 0d, 7d, 14d, 21d, 28d NDV HI, Immune Organs Index, lymhocyte transformation rate, the impact of Comprehensive Assessment Chinese medicine of the present invention on the Immune Profile In Chicks function.
2 results
2.1 antibody IgY against chicken Newcastle Disease bioactivity
Chinese drug-treated group antibody titer and blank group difference are not remarkable, illustrate that Chinese medicine is little on the impact of humoral immunity of organism, and this and early stage Chinese medicine kill virus, reduce antigenic stimulus relevant.In Table 5.
2.2 the mensuration Chinese drug-treated group fabricius bursa index of Immune Organs Index is lower than the blank group, but difference is not remarkable; And index and spleen index is significantly higher than the blank group; Thymus index is higher than matched group, but difference is not remarkable.This is consistent with above-mentioned conclusion.Chinese medicine had both had stronger cellular immunization enhancement function, and NDV virus is had again to stronger killing action, thus the fabricius bursa index that mainly participates in humoral immunization lower than the blank group, humoral immunization potentiation is not obvious.In Table 6.
2.3 lymphocyte transformation test
Chinese drug-treated group and left-handed rice azoles group all can significantly improve lymhocyte transformation rate, strengthen cellular immune function, improve resistance against diseases; Not remarkable with left-handed rice azoles group difference.In Table 6.
The impact that table 5 Chinese medicine is tired on antibody IgY against chicken Newcastle Disease
Annotate: the shoulder same difference is not remarkable, shoulder number different significant differences.
The impact of table 6 Chinese medicine on the impact (light absorption value of OD570nm) of Immune Organs of Chicken index and T total cellular score
Annotate: the shoulder same difference is not remarkable, shoulder number different significant differences.
Embodiment 4 acute toxicity tests
1 materials and methods
Select NIH kind mice, male and female half and half, body weight 17～19g.Gavage medicine through stomach.
2 results
Through prerun, give gavage Chinese medicine 0.4mg/20g body weight of mice (quite 100 times of the Adult Chicken consumption), all do not cause death, the concentration and the administration volume that are limited to medicine can not increase again, can not measure its LD
50therefore, carry out the maximum tolerance determination test.Select 20 of healthy mices, male and female half and half, minute 3 gavage the concentrated liquid of tradition Chinese medicines in 24 hours, only, accumulated dose reaches 85ml/Kg to each 0.8ml/, and this dosage is equivalent to 400 times of Adult Chicken consumption per day.Raise routinely after the mice administration and observe 10 days, have no dead and obvious poisoning symptom occurs.
3 conclusions
Through mtd test, the consumption of reaching 400 times of Adult Chickens to the dosage of mouse stomach Chinese medicine does not still cause death, shows that its acute toxicity is very little, oral safety, be can be applied to fully clinical.
Embodiment 5 chicken Newcastle disease protest tests
1 materials and methods
270 of SPF chickling 21 age in days SPF cocks (being provided by Shandong Province's safe Experimental Animal Center of sky), be divided into 3 groups at random, and 30 every group, every group of 3 repetitions.1,2 prevention groups, first 3 days of counteracting toxic substances by every day 0.25g/kg body weight and 0.5g/kg body weight add Chinese medicine in drinking-water.3,4 treatment groups: after manifest symptom appears in counteracting toxic substances, add Chinese medicine by 1.0g/kg body weight and 1.5g/kg body weight, continuous use 5d every day in drinking-water.5 groups is urgent inoculation group: after symptom appears in counteracting toxic substances, and with ND Lasota freeze dried vaccine, 6 times of urgent inoculations of amount.6 groups is the counteracting toxic substances matched group.NDV-F48E8 10
5eID
501000 times of dilutions, 0.1mL/ intramuscular injection.
1.2 criterion
The standard of morbidity: spirit is depressed, feed intake reduces, row's yellow green feces.
Clinical symptoms criterion: (1) is slight: row's yellow green feces, search for food, spirit is normal; (2) light: the symptom time of occurrence is slow, spirit is depressed, feed intake slightly subtracts; (3) heavy: as symptom time of occurrence morning, lethargy, to stand transfixed to the ground or sleeping ground does not rise, feed intake reduces or useless exhausted.
The prevention effect standard: (1) recovery from illness: tested chicken spirit, appetite, feces all recover normal; (2) effective: as except the chicken of curing fully belongs to effectively, through the good spirit of medication, appetite, feces, to recover to some extent, with matched group, compare the death time significant prolongation, can judge effectively; (3) invalid, after tested chicken medication, spirit, appetite, feces have no improvement, even increase the weight of death.
2 result of the tests
Table 7 Chinese medicine is to chicken artificial challenge ND prevention effect
As can be seen from Table 7: the protective rate of prevention group, treatment group and urgent inoculation group all is significantly higher than matched group, and clinical symptoms and the lesion degree of sick chicken are slighter.Especially prevention group can delay time and the death time that symptom occurs, and the highest protective rate reaches 93.3%, and cure rate reaches 83.3%.Preventive effect is better than therapeutic effect.
Embodiment 6 newcastle disease Clinical Treatment Tests
1 diagnostic criteria: clinical manifestation is for clearing green loose stool, and snore, stridulate, and turns round neck.Cut open inspection fiber nipple top hemorrhage, under the muscular stomach horny layer, striated is hemorrhage, and it is hemorrhage that the heart is preced with fatty tip-like, and the cecal tonsil enlargement is hemorrhage, and rectum and cloaca are hemorrhage etc.
2 administrated methods: medicine for treatment is 1.0g～1.5g/kg body weight every day, and drinking-water, 2 times/d, be used in conjunction 3～5d.
3 effects: medication 3d symptom basic controlling, death stops substantially, the 5d recovery from illness.The effective percentage of laying hen, broiler ND reaches respectively 98.8%, 96.5%.Refer to following table 8.
Table 8 newcastle disease Clinical Treatment Test result
Embodiment 2
Proportioning (weight portion): Flos Lonicerae 15, Folium Isatidis 15, Radix Isatidis 10, Radix Scutellariae 5, Fructus Forsythiae 10, Gypsum Fibrosum 18, the Rhizoma Anemarrhenae 8, Fructus Gardeniae 8, Radix Platycodonis 10, Semen Armeniacae Amarum (stir-fry) 5, Bulbus Fritillariae Thunbergii 5, Radix Glycyrrhizae 3.
Production technology is:
(1) soak: by the accurate weighing of above-mentioned weight portion, Flos Lonicerae, Folium Isatidis, Radix Scutellariae are added to 5 times of amount warm water (40～50 ℃) and soak 1 hour; Gypsum Fibrosum is smashed single bag; By Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii, Radix Glycyrrhizae, add 5 times of amount warm water (40～50 ℃) and soak 1 hour.
(2) decoct: Gypsum Fibrosum is decocted first 35 minutes, then adds the 8 flavor Chinese medicines such as Radix Isatidis, Fructus Forsythiae (adding together with soaking water) to boil 35 minutes, adds Flos Lonicerae, Folium Isatidis and Radix Scutellariae (adding together with soaking water) to boil 35 minutes again, filters; Filtering residue adds water (add warm water, amount of water is 3 times of crude drug gross weight before not soaking) and boils for the second time 1 hour, filters, and merges filtrate twice.
(3) concentrated: vacuum decompression (<60kpa) is concentrated into 1g crude drug/mL.
(4) anticorrosion: as to add 0.5% sodium benzoate anticorrosion.
(5) fill: when fluid temperature drops to 50 ℃, fill while stirring, sealing.
Embodiment 3
Proportioning (weight portion): Flos Lonicerae 10, Folium Isatidis 10, Radix Isatidis 12, Radix Scutellariae 9, Fructus Forsythiae 12, Gypsum Fibrosum 20, the Rhizoma Anemarrhenae 10, Fructus Gardeniae 10, Radix Platycodonis 8, Semen Armeniacae Amarum (stir-fry) 7, Bulbus Fritillariae Thunbergii 5, Radix Glycyrrhizae 5.
Production technology is:
(1) soak: take by weight each crude drug standby; Flos Lonicerae, Folium Isatidis, Radix Scutellariae are added to 7 times of amount warm water (40～50 ℃) to be soaked 1 hour; Gypsum Fibrosum is smashed single bag; Get Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii, Radix Glycyrrhizae, add 7 times of amount warm water (40～50 ℃) and soak 1 hour.
(2) decoct: Gypsum Fibrosum is decocted first 40 minutes, then adds the 8 flavor Chinese medicines such as Radix Isatidis, Fructus Forsythiae (adding together with soaking water) to boil 40 minutes, adds Flos Lonicerae, Folium Isatidis and Radix Scutellariae (adding together with soaking water) to boil 40 minutes again, filters; Boil for the second time 70min(and add warm water, amount of water is 5 times of crude drug gross weight before not soaking), filter, merge filtrate twice.
(3) concentrated: vacuum decompression (<60kpa) is concentrated into 1g crude drug/mL.
(4) anticorrosion: as to add 0.5% sodium benzoate anticorrosion.
(5) fill: when fluid temperature drops to 50 ℃, fill while stirring, sealing.
Claims (3)
1. a herbal mixture of preventing and treating newcastle disease, it is characterized in that, made by the crude drug of following weight parts: Flos Lonicerae 8～15, Folium Isatidis 5～15, Radix Isatidis 10～15, Radix Scutellariae 5～10, Fructus Forsythiae 10～15, Gypsum Fibrosum 18～22, the Rhizoma Anemarrhenae 8～12, Fructus Gardeniae 8～12, Radix Platycodonis 5～15, Semen Armeniacae Amarum 5～10, Bulbus Fritillariae Thunbergii 2～10, Radix Glycyrrhizae 3～5; Described Semen Armeniacae Amarum is Semen Armeniacae Amarum (parched); Its preparation method is:
(1) soak: take each crude drug by above-mentioned weight portion standby; Flos Lonicerae, Folium Isatidis are soaked together with Radix Scutellariae; Gypsum Fibrosum is smashed single bag; Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii are soaked together with Radix Glycyrrhizae;
(2) decoct: Gypsum Fibrosum is decocted first 30～50 minutes, then adds Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii and Radix Glycyrrhizae to boil 30～50 minutes, adds Flos Lonicerae, Folium Isatidis and Radix Scutellariae to boil 30～50 minutes again, filters; Filtering residue adds water boil 1～1.5 hour, filters, and merges filtrate twice;
(3) post processing: after after the filtrate vacuum-concentrcted, adding antiseptic, mixture is made in cooling fill.
2. a kind of herbal mixture of preventing and treating newcastle disease as claimed in claim 1, it is characterized in that, made by the crude drug of following weight parts: Flos Lonicerae 8, Folium Isatidis 8, Radix Isatidis 10, Radix Scutellariae 8, Fructus Forsythiae 10, Gypsum Fibrosum 19, the Rhizoma Anemarrhenae 10, Fructus Gardeniae 8, Radix Platycodonis 7, Semen Armeniacae Amarum 5, Bulbus Fritillariae Thunbergii 3, Radix Glycyrrhizae 4.
3. the described production technology of preventing and treating the herbal mixture of newcastle disease of claim 1 or 2, is characterized in that,
(1) soak: take each crude drug by the described weight portion of claim 1 or 2 standby; Flos Lonicerae, Folium Isatidis are soaked together with Radix Scutellariae; Gypsum Fibrosum is smashed single bag; Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii are soaked together with Radix Glycyrrhizae;
(2) decoct: Gypsum Fibrosum is decocted first 30～50 minutes, then adds Radix Isatidis, Fructus Forsythiae, the Rhizoma Anemarrhenae, Fructus Gardeniae, Radix Platycodonis, Semen Armeniacae Amarum, Bulbus Fritillariae Thunbergii and Radix Glycyrrhizae to boil 30～50 minutes, adds Flos Lonicerae, Folium Isatidis and Radix Scutellariae to boil 30～50 minutes again, filters; Filtering residue adds water boil 1～1.5 hour, filters, and merges filtrate twice;
(3) post processing: after after the filtrate vacuum-concentrcted, adding antiseptic, mixture is made in cooling fill.
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| CN103908565A (en) * | 2014-04-21 | 2014-07-09 | 张颖 | Traditional Chinese medicinal veterinary granules for preventing and treating atypical newcastle disease and preparation method thereof |
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| CN108853221A (en) * | 2018-09-19 | 2018-11-23 | 洛阳汉德生物科技有限公司 | A kind of drug and preparation method thereof for preventing and treating newcastle disease |
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| CN113244329A (en) * | 2021-06-18 | 2021-08-13 | 赵铭泽 | Traditional Chinese medicine compound preparation for treating poultry newcastle disease and preparation method thereof |
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