CN102754611B - Artificial control method for inducing synchronous maturing of gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent - Google Patents
Artificial control method for inducing synchronous maturing of gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent Download PDFInfo
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- CN102754611B CN102754611B CN201210243174.1A CN201210243174A CN102754611B CN 102754611 B CN102754611 B CN 102754611B CN 201210243174 A CN201210243174 A CN 201210243174A CN 102754611 B CN102754611 B CN 102754611B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention belongs to the field of aquaculture, and particularly relates to an artificial control method for inducing the synchronous maturing of the gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent. In the method, the male paralichthys dentatus parent is subjected to full-illumination environmental control in autumn and winter to suppress the gonad development of the male paralichthys dentatus parent; and in the spring of the next year, the full-illumination environment is changed into short-illumination environment, and the male paralichthys dentatus parent and the female paralichthys olivaceus parent are subjected to gonad development control at the same time to realize the gonad maturing of the male paralichthys dentatus parent. The method provided by the invention can obtain mature sperms and ova at the same time, prolongs the germ production period of the male paralichthys dentatus parent, increases the sperm quantity, and lays a technological base for obtaining batch high-quality sperms and ova in the large-scale offspring seed production of hybrid flounder.
Description
Technical field
The invention belongs to aquaculture field, is to extend male parent population by artificial regulatory envirment factor and hormone induction to produce the essence phase specifically, makes two kinds of fish reach artificial culture and the abductive approach of gonad maturity in same season.
Background technology
Summer flounder (Paralichthys dentatus) English is referred to as Summer flounder, also claims canine tooth lefteye flounder, Atlantic Ocean lefteye flounder, huge teeth flounder.Be distributed in North America the Atlantic coast, from the Maine State to Florida State.Comfort zone is 5-30 ℃, and suitable salt scope is that 5-35 ‰, pH accommodations are 6.0-8.2, and dissolved oxygen accommodation is 4-12mg/L.Summer flounder age at sexual maturity is more than 2.5 years, be to belong to the autumn and winter reproduction type when autumn, water temperature descended breeding period, from north to south, in the Atlantic Ocean, from September-coming year 2, monthlyly there is breeding individual, natural spawning water temperature 12-19 ℃, spawning period water temperature 15-18 ℃, breeding occurs in the migration way of summer flounder from nearshore waters to the wintering ground, deep-sea, and the breeding depth of water is many in the about 11-92m scope of the lower floor in cay waters.New York Bay sexual gland in October index reaches top, and North Carolina northern breeding period is the 9-12 month, and south is the 11-12 month, and the Atlantic coast in the middle part of N35-42, in breeding period 10-11 month, all can be bred from September-December by north orientation south.
Lefteye flounder (Paralichthys olivaceus), mainly be distributed in Bohai and Yellow Seas, the East Sea, the South Sea and Korea, Japan, littoral sea area, the Russia Far East.Comfort zone is 7-27 ℃, and suitable salt scope is that 8-33 ‰, pH accommodations are 7.0-8.2, and dissolved oxygen accommodation is 4-12mg/L.Be the 4-6 month breeding period of China's Huanghai Sea, Sea And Bohai Sea Coast lefteye flounder, and the Sheng phase is May, and the lefteye flounder that originates in the East Sea and the South Sea breeding period will be earlier.Early, on the south middle part, various places are the 2-5 month in the egg-laying period south of Japan's various places lefteye flounder, and sea of Japan bank is the 5-6 month, and northeast bank, gulf difficult to understand, land, Esfahan Bay are the 6-7 month.
Due to the summer flounder different season from the natural propagation of lefteye flounder, therefore, need to be under artificial regulatory and the condition of inducing, impel two kinds of fish parent populations at gonadal maturation in same season.Utilize male summer flounder and the hybridization of female lefteye flounder can obtain normotrophic filial generation, its culture trait obviously is better than parents; And utilize female summer flounder and the filial generation that obtains of male Japanese flounders hybridization have the chromosome deletion phenomenon, can not normal development.
Summary of the invention
The object of the present invention is to provide and a kind ofly induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland.
For achieving the above object, the technical solution used in the present invention is:
A kind ofly induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland: by male summer the flounder parent population in autumn and winter the environment conditioning by full exposure, suppress male summer flounder parent population gonad development, until proceed to short photoenvironment by the environment of full exposure spring in next year, now with female lefteye flounder parent population, carry out the gonad development regulation and control simultaneously, implement hormone induction after male summer flounder parent population gonadal maturation, extend the process of producing the essence phase.
Be specially:
(1) male summer flounder parent population and the cultivation of female lefteye flounder parent population: choose male summer flounder parent population and exist, water temperature 11-13 ℃, carry out full exposure under intensity of illumination 60-600Lux, then male parent population being gone to short illumination is cultivated, carry out female lefteye flounder parent fish rearing, until the sexual gland of male and female parent population is ripe respectively simultaneously;
(2) the flounder parent population produces prolongation and the artificial hybridization insemination of essence phase male summer: until above-mentioned gonad maturity the male summer, the flounder parent population can be extruded the milky seminal fluid time, in water temperature, be 17-19 ℃, illumination 10L/14D, HCG is extended to produce the essence phase and increase essence with 200-300IU/kg and induce, induce once at interval of 15 days, until female lefteye flounder parent population no longer lays eggs; Sperm after above-mentioned maturation and ovum are carried out to the artificial hybridization insemination.
Described step 1) is chosen male summer flounder parent population and is existed, water temperature 11-13 ℃, carry out full exposure under intensity of illumination 60-600Lux, then male parent population is gone to short illumination and cultivated, carry out female lefteye flounder parent fish rearing, until the sexual gland of male and female parent population is ripe respectively simultaneously;
When wherein male parent population being gone to short illumination and being cultivated, every day is shortened to 0.5-1 hour in illumination, transit to gradually the short illumination of 10L/14D, rising 1-1.5 ℃ of every three days of water temperature to temperature reaches 17-19 ℃, carries out male summer flounder parent population gonad maturity and cultivates;
Choose female lefteye flounder adult fish and carry out illumination, intensity of illumination is 60-600Lux, and light application time is that 12L/12D transits to the long illumination of 16L/8D gradually, and water temperature 14-16 ℃, realize the gonad maturity cultivation.
When described step 1) is carried out full exposure, to male summer flounder parent population sand lance of throwing something and feeding, throw something and feed twice every day, each feeding volume accounts for the 2-5% of parent population body weight; When described intensity of illumination transits to the short illumination of 10L/14D, throw something and feed and add the little yellow croaker of parent population nutrition fortifier, parent population nutrition fortifier adding proportion accounts for 3-5% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for the 5%-10% of parent population body weight, the secondary of throwing something and feeding day.The secondary of throwing something and feeding described parent population fortification day, be respectively 8:00 and 15:00.
Throw something and feed during the female lefteye flounder parent fish rearing of described step 1) and add Vc and V
elittle yellow croaker, wherein the Vc addition accounts for the 3-5% of little yellow croaker weight; V
eaddition accounts for the 1-2% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for the 5%-10% of parent population body weight, the secondary of throwing something and feeding day.The secondary of throwing something and feeding described parent population fortification day, be respectively 8:00 and 15:00.
The present invention has following advantage:
1. employing the inventive method can obtain ripe sperm and ovum simultaneously, and the exomixis rate reaches more than 90%;
2. employing the inventive method, can obtain and extend the male flounder parent population product essence phase in summer and increase the effect that essence is measured;
3. employing the inventive method, in the production of hybridization flounder scale seed, obtain high quality fertilized egg of scophthatmus in batches and established technical foundation.
Embodiment
The present invention is since September, and to male summer flounder parent population enforcement full exposure, water temperature is controlled at 11-13 ℃, to the March in next year, with female lefteye flounder parent population, carries out the gonad development regulation and control simultaneously.Summer, flounder shortened illumination in one hour from full exposure every day, to illumination every day 10 hours; Water temperature was once raising in every three days, to 17-19 ℃.The lefteye flounder breeding condition is: illumination every day 16 hours, water temperature is controlled at 14-16 ℃.When the male summer, flounder can be extruded the milky seminal fluid, HCG is induced with 250IU/kg, under the condition of illumination every day 10 hours and water temperature 17-19 ℃, at interval of 15 days, induce once, until female lefteye flounder parent population no longer lays eggs.
The present invention induce male summer flounder synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland be to pass through environment conditioning in autumn and winter, suppress male summer flounder parent population gonad development, until next year and female lefteye flounder parent population carry out the gonad development regulation and control simultaneously, implement hormone induction after male summer flounder parent population gonadal maturation, extend the process of producing the essence phase.
Embodiment:
(1) male summer flounder parent fish rearing: select above male summer in two ages flounder adult fish as male parent.Since autumn, be at Rizhao City marine living resources propagation station, to cultivate September.The parent fish rearing pond is 5.5 * 5.5 * 1.2m cement pit.Extract underground sea water, through precipitation, cooling, water temperature is controlled at 11 ℃, adopting light to control the parent fish pond surface light fully shines, intensity of illumination 500Lux, full exposure in 24 hours, a day quantity of exchanged water is 1 full dose, every day, each feeding volume accounted for 3% of parent population body weight respectively 8:00 and the 15:00 chilled sand lance of throwing something and feeding.Carry out the gonad maturity reinforced cultivating to March spring in next year, extract nature seawater and underground sea water, through precipitation, sand filtration, heating, adopting light to control the parent fish pond surface light shines, intensity of illumination 500Lux, light application time transits to the short illumination of 10L/14D by full exposure, and water temperature rises 1 ℃ in every three days until temperature reaches 17 ℃.Day quantity of exchanged water is 2 full doses.Throw something and feed and add the little yellow croaker of parent population nutrition fortifier (the precious A of the health that Qingdao Samuels Industrial & Commercial Co., Ltd. produces) at 8:00 and 15:00 every day respectively, parent population nutrition fortifier adding proportion is to account for 3% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for 6% of parent population body weight.
(2) female lefteye flounder parent fish rearing: more than selecting for three ages, female lefteye flounder adult fish is as female parent.Start parent population gonad maturity reinforced cultivating March spring in next year.The circular cement pit that the parent fish rearing pond is radius 3.5m.Extract nature seawater and underground sea water, through precipitation, sand filtration, heating, a day quantity of exchanged water is 2 full doses.Throw something and feed and add Vc and V
elittle yellow croaker, wherein the Vc addition accounts for 3% of little yellow croaker weight; V
eaddition accounts for 1% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for 8% of parent population body weight, the secondary (8:00 and 15:00) of throwing something and feeding day.Adopt light to control parent fish pond surface light photograph fully, intensity of illumination 600Lux, light application time is that 12L/12D transits to the long illumination of 16L/8D, 15 ℃ of water temperatures, and then realize that two parent population simultaneity gland maturities carry out the gonad development regulation and control.
(3) then respectively in April 15, May 1, May 16, May 31, June 15, select the male summer flounder parent population that can extrude the milky seminal fluid, water temperature is 18 ℃, illumination 10L/14D, HCG is extended to produce the essence phase and increase essence with the 250IU/kg formula and induce, until female lefteye flounder parent population no longer lays eggs.
(4) artificial hybridization insemination: select the adult female lefteye flounder, extrude mature egg along gonad by before backward with hand, summer flounder seminal fluid (frozen semen or collect in advance and be stored in 4 ℃ of refrigerators) is splashed into, add several sea-water activated, constantly stir 3 minutes, add seawater full to basin, standing 5 minutes again, with 80 mesh sieve tulles, filter, use sand filtration seawater flushing fertilized egg 3 times, fertilized egg is put into to the 2000ml graduated cylinder standing, get the floating ovum and hatched.By the float ratio measuring fertilization rate of ovum and total ovum amount of calculating, fertilization rate average out to 90.2%.
Claims (7)
1. induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland for one kind, it is characterized in that: by male summer the flounder parent population in autumn and winter the environment conditioning by full exposure, suppress male summer flounder parent population gonad development, until proceed to short photoenvironment by the environment of full exposure spring in next year, now with female lefteye flounder parent population, carry out the gonad development regulation and control simultaneously, realize male summer flounder parent population gonad maturity;
The intensity of illumination of described full exposure is 60-600Lux.
2. by claimed in claim 1, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that:
(1) male summer flounder parent population and the cultivation of female lefteye flounder parent population: choose male summer flounder parent population and exist, water temperature 11-13 ℃, carry out full exposure under intensity of illumination 60-600Lux, then male parent population being gone to short illumination is cultivated, carry out female lefteye flounder parent fish rearing, until the sexual gland of male and female parent population is ripe respectively simultaneously;
(2) the flounder parent population produces prolongation and the artificial hybridization insemination of essence phase male summer: until above-mentioned gonad maturity the male summer, the flounder parent population can be extruded the milky seminal fluid time, in water temperature, be 17-19 ℃, illumination 10L/14D, HCG is extended to produce the essence phase and increase essence with 200-300IU/kg and induce, induce once at interval of 15 days, until female lefteye flounder parent population no longer lays eggs; Sperm after above-mentioned maturation and ovum are carried out to the artificial hybridization insemination.
3. by claimed in claim 2, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that:
Described step 1) is chosen male summer flounder parent population and is existed, water temperature 11-13 ℃, carry out full exposure under intensity of illumination 60-600Lux, then male parent population is gone to short illumination and cultivated, carry out female lefteye flounder parent fish rearing, until the sexual gland of male and female parent population is ripe respectively simultaneously;
When wherein male parent population being gone to short illumination and being cultivated, every day is shortened to 0.5-1 hour in illumination, transit to gradually the short illumination of 10L/14D, rising 1-1.5 ℃ of every three days of water temperature to temperature reaches 17-19 ℃, carries out male summer flounder parent population gonad maturity and cultivates;
Choose female lefteye flounder adult fish and carry out illumination, intensity of illumination is 60-600Lux, and light application time is that 12L/12D transits to the long illumination of 16L/8D gradually, and water temperature 14-16 ℃, realize the gonad maturity cultivation.
4. by claimed in claim 1, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that: when described step 1) is carried out full exposure, to male summer flounder parent population sand lance of throwing something and feeding, throw something and feed twice every day, each feeding volume accounts for the 2-5% of parent population body weight; When described intensity of illumination transits to the short illumination of 10L/14D, throw something and feed and add the little yellow croaker of parent population nutrition fortifier, parent population nutrition fortifier adding proportion accounts for 3-5% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for the 5%-10% of parent population body weight, the secondary of throwing something and feeding day.
5. by claimed in claim 4, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that: the secondary of throwing something and feeding described parent population fortification day is respectively 8:00 and 15:00.
6. by claimed in claim 1, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that: throw something and feed during the female lefteye flounder parent fish rearing of described step 1) and add Vc and V
elittle yellow croaker, wherein the Vc addition accounts for the 3-5% of little yellow croaker weight; V
eaddition accounts for the 1-2% of little yellow croaker weight, and the feeding volume of each little yellow croaker accounts for the 5%-10% of parent population body weight, the secondary of throwing something and feeding day.
7. by claimed in claim 6, induce male summer flounder to synchronize ripe artificial regulatory method with female lefteye flounder parent population sexual gland, it is characterized in that: the secondary of throwing something and feeding described parent population fortification day is respectively 8:00 and 15:00.
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| CN201210243174.1A CN102754611B (en) | 2012-07-12 | 2012-07-12 | Artificial control method for inducing synchronous maturing of gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent |
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| CN201210243174.1A CN102754611B (en) | 2012-07-12 | 2012-07-12 | Artificial control method for inducing synchronous maturing of gonads of male paralichthys dentatus parent and female paralichthys olivaceus parent |
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| CN116941551B (en) * | 2023-08-25 | 2023-12-29 | 中国科学院水生生物研究所 | Temperature treatment method for inducing annual maturation of female carp of yellow river |
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