CN102742569A - Preparation method for giant salamander semen preserving agent - Google Patents
Preparation method for giant salamander semen preserving agent Download PDFInfo
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- CN102742569A CN102742569A CN2012102776732A CN201210277673A CN102742569A CN 102742569 A CN102742569 A CN 102742569A CN 2012102776732 A CN2012102776732 A CN 2012102776732A CN 201210277673 A CN201210277673 A CN 201210277673A CN 102742569 A CN102742569 A CN 102742569A
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- giant salamander
- seminal fluid
- chloride
- preserving agent
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- 241000157862 Dicamptodontidae Species 0.000 title claims abstract description 59
- 210000000582 semen Anatomy 0.000 title claims abstract description 41
- 239000003755 preservative agent Substances 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 10
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 7
- 239000011780 sodium chloride Substances 0.000 claims abstract description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims abstract description 5
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims abstract description 5
- 239000001110 calcium chloride Substances 0.000 claims abstract description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 claims abstract description 5
- 235000019800 disodium phosphate Nutrition 0.000 claims abstract description 5
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 5
- 239000011565 manganese chloride Substances 0.000 claims abstract description 5
- 235000002867 manganese chloride Nutrition 0.000 claims abstract description 5
- 229940099607 manganese chloride Drugs 0.000 claims abstract description 5
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims abstract description 5
- 235000019799 monosodium phosphate Nutrition 0.000 claims abstract description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 5
- 235000017550 sodium carbonate Nutrition 0.000 claims abstract description 5
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 5
- 239000011592 zinc chloride Substances 0.000 claims abstract description 5
- 235000005074 zinc chloride Nutrition 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 3
- 238000010790 dilution Methods 0.000 claims description 26
- 239000012895 dilution Substances 0.000 claims description 26
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 14
- 240000002900 Arthrospira platensis Species 0.000 claims description 14
- 229940082787 spirulina Drugs 0.000 claims description 14
- 150000004676 glycans Chemical class 0.000 claims description 13
- 229920001282 polysaccharide Polymers 0.000 claims description 13
- 239000005017 polysaccharide Substances 0.000 claims description 13
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 229930003316 Vitamin D Natural products 0.000 claims description 5
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 5
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
- 210000002149 gonad Anatomy 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 235000019166 vitamin D Nutrition 0.000 claims description 5
- 239000011710 vitamin D Substances 0.000 claims description 5
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 5
- 235000019165 vitamin E Nutrition 0.000 claims description 5
- 229940046009 vitamin E Drugs 0.000 claims description 5
- 239000011709 vitamin E Substances 0.000 claims description 5
- 229940046008 vitamin d Drugs 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 4
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
- 235000010355 mannitol Nutrition 0.000 claims description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 10
- 230000001488 breeding effect Effects 0.000 abstract description 10
- 235000002639 sodium chloride Nutrition 0.000 abstract description 5
- 235000011148 calcium chloride Nutrition 0.000 abstract description 4
- 235000011147 magnesium chloride Nutrition 0.000 abstract description 4
- 229910021578 Iron(III) chloride Inorganic materials 0.000 abstract 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 abstract 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 description 9
- 108010000912 Egg Proteins Proteins 0.000 description 9
- 210000004681 ovum Anatomy 0.000 description 9
- 230000000694 effects Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 230000037396 body weight Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 230000009027 insemination Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 229910052691 Erbium Inorganic materials 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- UYAHIZSMUZPPFV-UHFFFAOYSA-N erbium Chemical compound [Er] UYAHIZSMUZPPFV-UHFFFAOYSA-N 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 241000269333 Caudata Species 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 241000361919 Metaphire sieboldi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000019155 Radiation injury Diseases 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000011013 aquamarine Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
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- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009318 large scale farming Methods 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 210000003887 myelocyte Anatomy 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a giant salamander semen preserving agent which comprises sodium chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, sodium bicarbonate, sodium carbonate, ferric chloride, calcium chloride, magnesium chloride, zinc chloride, manganese chloride and the like, and the balance water. A preparation method of the giant salamander semen preserving agent is simple in process, low in cost and high in operability and greatly improves breeding rate of giant salamander.
Description
Technical field
The present invention relates to the producing method for seed of a kind of fish, particularly relate to a kind of preparation method of giant salamander seminal fluid preserving agent, this technology belongs to biological technical field.
Background technology
Giant salamander is commonly called as the giant salamander, is China institute special product, belongs to animals on the brink of extinction, is classified as two types by country and lays special stress on protecting wildlife.Through large-scale farming in recent years, giant salamander germ plasm resource is degenerated serious, and growth rate significantly descends, and various diseases takes place frequently.Carry out the seed selection of giant salamander superior families, significant for increasing economic efficiency etc.
Owing to plant costing an arm and a leg of salamander; The artificial breeding giant salamander; Environment, equipment requirements are high, technical difficulty is big, therefore will culture commodity giant salamander and the raiser who lacks experience, and must arrive that aqua-marine life is raised and train breeding and operation utilizes the regular breeding field of licensing to obtain the giant salamander seed.In order to improve the survival rate of giant salamander, should select to hatch emerge 3 months, the long giant salamander seedling more than 5 centimetres of body.The giant salamander seedling is in transportation, and the activity of temperature and giant salamander, oxygen demand etc. have confidential relation.When adopting the oxygenation transportation, take advantage of in 40 the bag 40,6-12 centimetre seedling can not surpass 60 tails at most.Transportation giant salamander seedling breeds not as transportation giant salamander seminal fluid, therefore, and the special economically valuable of exploitation giant salamander seminal fluid preserving agent.
Summary of the invention
The present invention aims to provide a kind of preparation method of giant salamander seminal fluid preserving agent.
The preserving agent of giant salamander seminal fluid, the composition of this preserving agent is:
NaCl 30-60 mmol/L, NaH
2PO
42-5 mmol/L, Na
2HPO
44-10 mmol/L; NaHCO
32-5 mmol/L, Na
2CO
34-10 mmol/L, FeCl
36-12 mmol/L, CaCl
28-16 mmol/L, MgCl
216-32 mmol/L, ZnCl
216-32 mmol/L, MnCl
21-2 mmol/L, natrium adetate 2-5 mmol/L, vitamin C 2-5 mmol/ L, vitamin D 2-5 mmol/ L, vitamin E 2-5 mmol/ L, mannitol 100-200 mmol/L, spirulina polysaccharide 100-200 mmol/L, all the other are water.
Sodium chloride 30-60 mmol/L, sodium dihydrogen phosphate 2-5 mmol/L, sodium hydrogen phosphate
4-10 mmol/L; Sodium bicarbonate 2-5 mmol/L, sodium carbonate 4-10 mmol/L, iron chloride 6-12 mmol/L, calcium chloride 8-16 mmol/L; Magnesium chloride 16-32 mmol/L, zinc chloride 16-32 mmol/L, manganese chloride 1-2 mmol/L, natrium adetate 2-5 mmol/L; Vitamin C 2-5 mmol/ L, vitamin D 2-5 mmol/ L, vitamin E 2-5 mmol/ L; Mannitol 100-200 mmol/L, spirulina polysaccharide 100-200 mmol/L, all the other are water.
With the proportionally weighing of above composition, and the water dissolving, constant volume.
2. the specific process of a kind of preservation giant salamander seminal fluid of the present invention is:
1) chooses the giant salamander milter of gonad maturity, gather ripe seminal fluid;
2) preserving agent with claim 1 dilutes the giant salamander seminal fluid;
Dilution time is according to, giant salamander seminal fluid: dilution is with " 1-3 ": the dilution proportion of " 15-45 "; In the time of dilution: temperature is 10-15 ℃.
Spirulina polysaccharide:, nontoxic from the physiologically active polysaccharide that spirulina extracts.Proterties: colourless or pale yellow powder.Water-soluble, be insoluble to pure and mild grease.Quality index: outward appearance: colourless or pale yellow powder.
Spirulina polysaccharide: through suppressing free-radical oxidation reaction in the body, reduce lipid peroxidation, reduce the formation of organizing lipofuscin, can reach the effect that slows down body aging.
The spirulina polysaccharide ability: enhance bone myelocyte proliferation activity, promotion DNA repair synthesis, the good resistance radiation function is arranged and reduce radiotherapy, side effects of chemotherapy, and ionization radiation injury is had tangible protection effect.
Spirulina polysaccharide ability: improve the nonspecific cellular immune function of body, and can promote body to change different in nature humoral immune function, improve low ability of driving germ, poisoning intrusion.
The spirulina polysaccharide ability: promote immune organs growths such as thymus gland, spleen gland, promote gpv protein synthetic, the elimination immunodepressant is to immune inhibitory action.
A kind of method for distilling of spirulina polysaccharide includes, without being limited to: spirulina raw material immersed in the water, is placed in-15 ℃ the ice freezing 72 hours after stirring, take out quick thawing, and centrifugal filtration, algae-residue extracts spirulina polysaccharide; Add Na in the filter residue
2CO
3Be dissolved into solution, centrifugal filtration discards filter residue, keeps filtrating. and regulate filtrating pH to neutral, concentrate, atomized drying.
Giant salamander breeding population of the present invention is cultivated and genital regulating:
The giant salamander breeding population is set up the breeding archives,, reach the 2-3 monthly age propagating concentrated raising cultivation under the condition artificially.The year of breeding population cultivates temperature and is controlled at 12-24 ℃, adopts normal sunshine, and mating season, water temperature was controlled at 10-15 ℃, light application time 6-8h, and intensity of illumination 800-1200lux, the Continuous irradiation 0.5-1 month, the water flow exchange amount is 2-3m
3/ h,
Parent population utilizes commercially available erbium material to feed at anestrous season,
Utilize mating season the commercially available erbium material that adds 5% earthworm to feed.
Reach sexual maturity, output mature egg and sperm through above cultivation.
Semen collection and transportation
Select sexually matured milter, adopt belly extrusion collecting semen, dry, draw milky white seminal fluid, do not draw the yellow urine of discharging with glass pipette with around the milter cloacal aperture.The seminal fluid of gathering is all contained in the vial of drying of 20ml, the vial that seminal fluid is housed is placed on ice.Seminal fluid is avoided the light direct projection in transporting the way.
The method that the giant salamander seminal fluid of long-distance transport is used to breed
1) chooses the giant salamander milter and the raun of gonad maturity, gather ripe seminal fluid and ovum respectively;
2) with preserving agent dilution giant salamander seminal fluid of the present invention.
Dilution time is according to, giant salamander seminal fluid: dilution is with (1-3) ml: (15-45) dilution proportion of ml; In the time of dilution: temperature is 10-15 ℃.
The giant salamander seminal fluid of dilution is evenly mixed with the ovum grain, and ovum grain and seminal fluid volume ratio are (15-30) ml: (0.1-0.5) ml, and stir the back and leave standstill 5-20min for 10-15 ℃, add an amount of 10-15 ℃ of streams water artificial insemination.
3) acquisition of giant salamander fry: fertilized egg moved in the 10-15 ℃ of streams water hatch,, cultivate the giant salamander fry according to conventional giant salamander seedling and seed production process management.
The cultural method of giant salamander fry
At 4 1m
3In the water vat, raise respectively, use miniflow water in early days, every day exchange capacity 0.3m
3, keep water quality pure and fresh, every day, each precipitated at the bottom of removing the pond for 1 time sooner or later.
Put newly hatched larvae quantity in a suitable place to breed about 4000 tails in the 1st water vat, do not throw in pellet
Put volt ground fish seedling (the long 1cm of body) 2000 tails in the 2nd water vat in a suitable place to breed, pellet particle diameter 80-100 μ m,
Put 5-10cm fry 1000 tails in a suitable place to breed in the 3rd water vat, pellet particle diameter 200-400 μ m,
Put 20-25cm fry 500 tails in a suitable place to breed in the 4th water vat.About pellet particle diameter 800 μ m.
In the water body of 1-4 water vat, ten thousand/ml of spirulina feeding volume 10-20, wheel animalcule feeding volume 10-20/ml, halogen worm feeding volume 5-10/ml.
Embodiment
Specific embodiment 1
1) chooses the giant salamander milter and the raun of gonad maturity, gather ripe seminal fluid and ovum respectively;
2) with preserving agent dilution giant salamander seminal fluid of the present invention.
Sodium chloride 30mmol/L, sodium dihydrogen phosphate 2mmol/L, sodium hydrogen phosphate
4 mmol/L; Sodium bicarbonate 2mmol/L, sodium carbonate 4 mmol/L, iron chloride 6mmol/L, calcium chloride 8 mmol/L; Magnesium chloride 16mmol/L, zinc chloride 16 mmol/L, manganese chloride 1mmol/L, natrium adetate 2 mmol/L; Vitamin C 2mmol/ L, vitamin D 2mmol/ L, vitamin E 2 mmol/ L; Mannitol 100 mmol/L, spirulina polysaccharide 100 mmol/L, all the other are water.With the proportionally weighing of above composition, and the water dissolving, constant volume.
Dilution time is according to, giant salamander seminal fluid: dilution is with (1) ml: the dilution proportion of (15) ml; In the time of dilution: temperature is 10 ℃.
The giant salamander seminal fluid of dilution is evenly mixed with the ovum grain, and ovum grain and seminal fluid volume ratio are (15) ml: (0.1) ml, and stir back 10 ℃ and leave standstill 10min, add an amount of 10 ℃ of streams water artificial inseminations.
3) acquisition of giant salamander fry: fertilized egg moved in 10 ℃ of streams water hatch,, cultivate the giant salamander fry according to conventional giant salamander seedling and seed production process management.
Set up giant salamander fry kind system: measure, body length was 10.21-15.47cm in 30 days, and body weight is 25.56-27.34g, and growth is fast; Survival rate is up to 79.3%, and premunition is strong, obtains 30 world with conventional breeding and compares; Body is long by 10.8%, and body weight is high by 15.5%, and survival rate is high by 12.9%.
Specific embodiment 2
1) chooses the giant salamander milter and the raun of gonad maturity, gather ripe seminal fluid and ovum respectively;
2) with preserving agent dilution giant salamander seminal fluid of the present invention.
Sodium chloride 60 mmol/L, sodium dihydrogen phosphate 5 mmol/L, sodium hydrogen phosphate
10 mmol/L; Sodium bicarbonate 5 mmol/L, sodium carbonate 10 mmol/L, iron chloride 12 mmol/L, calcium chloride 16 mmol/L; Magnesium chloride 32 mmol/L, zinc chloride 32 mmol/L, manganese chloride 2 mmol/L, natrium adetate 5 mmol/L; Vitamin C 5 mmol/ L, vitamin D 5 mmol/ L, vitamin E 5 mmol/ L; Mannitol 200 mmol/L, spirulina polysaccharide 200 mmol/L, all the other are water.With the proportionally weighing of above composition, and the water dissolving, constant volume.
Dilution time is according to, giant salamander seminal fluid: dilution is with (2) ml: the dilution proportion of (40) ml; In the time of dilution: temperature is 15 ℃.
The giant salamander seminal fluid of dilution is evenly mixed with the ovum grain, and ovum grain and seminal fluid volume ratio are (30) ml: (0.5) ml, and stir back 15 ℃ and leave standstill 20min, add an amount of 15 ℃ of streams water artificial inseminations.
3) acquisition of giant salamander fry: fertilized egg moved in 15 ℃ of streams water hatch,, cultivate the giant salamander fry according to conventional giant salamander seedling and seed production process management.
Set up giant salamander fry kind system: measure, body length was 12.19-17.67cm in 30 days, and body weight is 24.39-29.21g, and growth is fast; Survival rate is up to 79.3%, and premunition is strong, obtains 30 world with conventional breeding and compares; Body is long by 12.1%, and body weight is high by 16.7%, and survival rate is high by 13.1%.
Claims (3)
1. the preserving agent of giant salamander seminal fluid, the composition of this preserving agent is:
Sodium chloride 30-60 mmol/L, sodium dihydrogen phosphate 2-5 mmol/L, sodium hydrogen phosphate
4-10 mmol/L; Sodium bicarbonate 2-5 mmol/L, sodium carbonate 4-10 mmol/L, iron chloride 6-12 mmol/L, calcium chloride 8-16 mmol/L; Magnesium chloride 16-32 mmol/L, zinc chloride 16-32 mmol/L, manganese chloride 1-2 mmol/L, natrium adetate 2-5 mmol/L; Vitamin C 2-5 mmol/ L, vitamin D 2-5 mmol/ L, vitamin E 2-5 mmol/ L; Mannitol 100-200 mmol/L, spirulina polysaccharide 100-200 mmol/L, all the other are water.
2. the preparation method of the preserving agent of giant salamander seminal fluid of the present invention, with the proportionally weighing of composition of claim 1, and the water dissolving, constant volume.
3. the specific process of a kind of preservation giant salamander seminal fluid of the present invention is characterized in that:
(1) chooses the giant salamander milter of gonad maturity, gather ripe seminal fluid;
(2) preserving agent with claim 2 dilutes the giant salamander seminal fluid; Dilution time is according to, giant salamander seminal fluid: dilution is with " 1-3 ": the dilution proportion of " 15-45 "; In the time of dilution: temperature is 10-15 ℃.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210277673 CN102742569B (en) | 2012-01-15 | 2012-08-07 | Preparation method for giant salamander semen preserving agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210010658.1 | 2012-01-15 | ||
| CN201210010658 | 2012-01-15 | ||
| CN 201210277673 CN102742569B (en) | 2012-01-15 | 2012-08-07 | Preparation method for giant salamander semen preserving agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102742569A true CN102742569A (en) | 2012-10-24 |
| CN102742569B CN102742569B (en) | 2013-11-06 |
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|---|---|---|---|
| CN 201210277673 Expired - Fee Related CN102742569B (en) | 2012-01-15 | 2012-08-07 | Preparation method for giant salamander semen preserving agent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212945A (en) * | 2017-06-20 | 2017-09-29 | 金寨县胜华娃娃鱼开发有限公司 | Preparation and application for the high-efficiency agent for dilution liquid of giant salamander seminal fluid |
| CN110301433A (en) * | 2019-07-25 | 2019-10-08 | 河南精怡达生物科技有限公司 | A kind of pig semen containing trace and REE elements is stored at room temperature agent in vitro |
| CN111183975A (en) * | 2020-02-21 | 2020-05-22 | 海南晨海水产有限公司 | Formula of rockfish sperm refrigerating fluid with high survival rate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002012441A2 (en) * | 2000-08-10 | 2002-02-14 | Genzyme Transgenics Corporation | Cryopreservation of sperm |
| CN102217590A (en) * | 2011-08-01 | 2011-10-19 | 天津科技大学 | Pomfret sperm cryopreservation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002012441A2 (en) * | 2000-08-10 | 2002-02-14 | Genzyme Transgenics Corporation | Cryopreservation of sperm |
| CN102217590A (en) * | 2011-08-01 | 2011-10-19 | 天津科技大学 | Pomfret sperm cryopreservation method |
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| 姚桂东 等: "维生素B12、E和C在家畜精液保存中的应用", 《动物医学进展》, vol. 27, no. 3, 20 March 2006 (2006-03-20) * |
| 邓岳松: "鱼类精液的低温保存", 《水利渔业》, vol. 19, no. 4, 25 July 1999 (1999-07-25) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212945A (en) * | 2017-06-20 | 2017-09-29 | 金寨县胜华娃娃鱼开发有限公司 | Preparation and application for the high-efficiency agent for dilution liquid of giant salamander seminal fluid |
| CN110301433A (en) * | 2019-07-25 | 2019-10-08 | 河南精怡达生物科技有限公司 | A kind of pig semen containing trace and REE elements is stored at room temperature agent in vitro |
| CN111183975A (en) * | 2020-02-21 | 2020-05-22 | 海南晨海水产有限公司 | Formula of rockfish sperm refrigerating fluid with high survival rate |
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| CN102742569B (en) | 2013-11-06 |
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