CN102690809B - Dna标签及其在构建和测序配对末端标签文库中的应用 - Google Patents
Dna标签及其在构建和测序配对末端标签文库中的应用 Download PDFInfo
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- CN102690809B CN102690809B CN2011100711762A CN201110071176A CN102690809B CN 102690809 B CN102690809 B CN 102690809B CN 2011100711762 A CN2011100711762 A CN 2011100711762A CN 201110071176 A CN201110071176 A CN 201110071176A CN 102690809 B CN102690809 B CN 102690809B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
Description
SEQ ID NO: | 名称 | 碱基序列 | 荧光信号序列 |
1 | Index1 | GGAAG | 00202 |
2 | Index2 | TCATG | 12131 |
3 | Index3 | CAAGG | 31020 |
4 | Index4 | ATACG | 23313 |
5 | Index5 | AGCCG | 22303 |
6 | Index6 | TTCGG | 10230 |
7 | Index7 | GCCAG | 03012 |
8 | Index8 | CACTG | 31121 |
9 | Index9 | TTCTG | 10221 |
10 | Index10 | CGCCG | 33303 |
11 | Index11 | GACAG | 02112 |
12 | Index12 | ACCGG | 21030 |
13 | Index13 | CGATG | 33231 |
14 | Index14 | ACCCG | 21003 |
15 | Index15 | TCGAG | 12322 |
16 | Index16 | GGTGG | 00110 |
17 | Index17 | ACGTG | 21311 |
18 | Index18 | TCAGG | 12120 |
19 | Index19 | GCCCG | 03003 |
20 | Index20 | CCTAG | 30232 |
21 | Index21 | AAGAG | 20222 |
22 | Index22 | GATGG | 02310 |
23 | Index23 | TAATG | 13031 |
24 | Index24 | CACCG | 31103 |
Claims (29)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2011100711762A CN102690809B (zh) | 2011-03-24 | 2011-03-24 | Dna标签及其在构建和测序配对末端标签文库中的应用 |
PCT/CN2012/072970 WO2012126398A1 (zh) | 2011-03-24 | 2012-03-23 | Dna标签及其用途 |
HK13102245.4A HK1175196A1 (en) | 2011-03-24 | 2013-02-21 | Dna indexes and applications thereof on the mate-pair library construction and sequencing dna |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN2011100711762A CN102690809B (zh) | 2011-03-24 | 2011-03-24 | Dna标签及其在构建和测序配对末端标签文库中的应用 |
Publications (2)
Publication Number | Publication Date |
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CN102690809A CN102690809A (zh) | 2012-09-26 |
CN102690809B true CN102690809B (zh) | 2013-12-04 |
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CN2011100711762A Active CN102690809B (zh) | 2011-03-24 | 2011-03-24 | Dna标签及其在构建和测序配对末端标签文库中的应用 |
Country Status (3)
Country | Link |
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CN (1) | CN102690809B (zh) |
HK (1) | HK1175196A1 (zh) |
WO (1) | WO2012126398A1 (zh) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104630211B (zh) * | 2013-11-15 | 2018-03-02 | 苏州吉玛基因股份有限公司 | 一种Small RNA cDNA文库的构建方法 |
CN104695027B (zh) * | 2013-12-06 | 2017-10-20 | 中国科学院北京基因组研究所 | 测序文库及其制备和应用 |
CN104232760B (zh) * | 2014-08-26 | 2017-03-15 | 深圳华大基因医学有限公司 | 确定混合测序数据中读段的样本源的方法及装置 |
CN105420348B (zh) * | 2014-09-04 | 2019-10-15 | 中国科学院北京基因组研究所 | 改进的测序文库及其制备和应用 |
CN106795650B (zh) * | 2014-09-26 | 2021-03-09 | 深圳华大基因股份有限公司 | Pf快速建库方法及其应用 |
CN105525357B (zh) * | 2014-09-30 | 2018-08-21 | 深圳华大基因股份有限公司 | 一种测序文库的构建方法及试剂盒和应用 |
CN105154444A (zh) * | 2015-10-15 | 2015-12-16 | 南京普东兴生物科技有限公司 | 一种有效提高建库效率的非对称高通量测序接头及其应用 |
US20170298427A1 (en) * | 2015-11-16 | 2017-10-19 | Progenity, Inc. | Nucleic acids and methods for detecting methylation status |
CN105926043B (zh) * | 2016-04-19 | 2018-08-28 | 苏州贝康医疗器械有限公司 | 一种提高孕妇血浆游离dna测序文库中胎儿游离dna占比的方法 |
CN109576800A (zh) * | 2018-12-07 | 2019-04-05 | 北京安智因生物技术有限公司 | 一种遗传性扩张型心肌病的基因检测文库的构建方法及其试剂盒 |
CN111462818B (zh) * | 2019-01-22 | 2023-04-21 | 武汉华大医学检验所有限公司 | 测序产量预测方法和建立测序产量预测模型的方法及装置 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002103013A2 (en) * | 2000-10-30 | 2002-12-27 | Gene Logic, Inc. | Partially double-stranded nucleic acids, methods of making, and use thereof |
CN101921748A (zh) * | 2010-06-30 | 2010-12-22 | 深圳华大基因科技有限公司 | 用于高通量检测人类乳头瘤病毒的dna分子标签 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008045575A2 (en) * | 2006-10-13 | 2008-04-17 | J. Craig Venter Institute, Inc. | Sequencing method |
CN101434988B (zh) * | 2007-11-16 | 2013-05-01 | 深圳华因康基因科技有限公司 | 一种高通量寡核苷酸测序方法 |
CN101921840B (zh) * | 2010-06-30 | 2014-06-25 | 深圳华大基因科技有限公司 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
CN101967476B (zh) * | 2010-09-21 | 2012-11-14 | 深圳华大基因科技有限公司 | 一种基于接头连接的DNA PCR-Free标签文库构建方法 |
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2011
- 2011-03-24 CN CN2011100711762A patent/CN102690809B/zh active Active
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2012
- 2012-03-23 WO PCT/CN2012/072970 patent/WO2012126398A1/zh active Application Filing
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2013
- 2013-02-21 HK HK13102245.4A patent/HK1175196A1/xx unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002103013A2 (en) * | 2000-10-30 | 2002-12-27 | Gene Logic, Inc. | Partially double-stranded nucleic acids, methods of making, and use thereof |
CN101921748A (zh) * | 2010-06-30 | 2010-12-22 | 深圳华大基因科技有限公司 | 用于高通量检测人类乳头瘤病毒的dna分子标签 |
Non-Patent Citations (4)
Title |
---|
SSR分子标记的开发技术研究进展;唐荣华等;《西南农业学报》;20021230;第15卷(第04期);106-109 * |
唐荣华等.SSR分子标记的开发技术研究进展.《西南农业学报》.2002,第15卷(第04期),106-109. |
桥式PCR,一种简易连接DNA标签序列的方法(英文);毛建平等;《中国生物工程杂志》;20091115;第29卷(第11期);66-69 * |
毛建平等.桥式PCR 一种简易连接DNA标签序列的方法(英文).《中国生物工程杂志》.2009 |
Also Published As
Publication number | Publication date |
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WO2012126398A1 (zh) | 2012-09-27 |
CN102690809A (zh) | 2012-09-26 |
HK1175196A1 (en) | 2013-06-28 |
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