CN102670763A - Composition with auxiliary protection effect on chemical liver injury and preparation method of composition - Google Patents
Composition with auxiliary protection effect on chemical liver injury and preparation method of composition Download PDFInfo
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Abstract
The invention relates to a composition with an auxiliary protection effect on chemical liver injury and a preparation method of the composition. The composition with the auxiliary protection effect on the chemical liver injury comprises the following ingredients in parts by weight: 1 to 10 parts of schisandra chinensis, 1 to 15 parts of kudzuvine root, 1 to 10 parts of hovenia dulcis thumb and 0.1 to 10 parts of liquorice. The composition provided by the invention belongs to a composition which is screened on the basis of the existing traditional Chinese medicine theory and has the auxiliary protection effect on the chemical liver injury, through scientific matching, the mutual synergistic effect is realized, and experiments prove that the composition belongs to the composition with the auxiliary protection effect on the chemical liver injury and can be further prepared into health-care food or medicine with the auxiliary protection effect on the chemical liver injury.
Description
Technical field
The invention belongs to health food and medicine field, relate to a kind of composition and method of making the same that chemical liver injury is had auxiliary protection function.
Background technology
Hepatic injury is the clinical common healthy disease of harm humans, and the pathological factor that hepatic injury relates to is more extensive, like virus, biology, medicine, physics and chemistry, ethanol etc., can cause hepatic injury.
Chemical liver injury is the hepatic injury that is caused by the chemical Hepatoxic substance.These chemical substances comprise chemical toxicant and the some drugs in ethanol, the environment.Liver as the important detoxifcation organ of human body has Hepatic artery and hepatic vein double blood supply.Chemical substance can get into liver through gastrointestinal tract portal vein or body circulation and transform, so liver receives the toxicant infringement in the chemicals easily.All exist some to the virose material of liver in the Nature and the human industrial processes; Be called " hepatotropic poison "; These poisonous substances are general susceptible in the crowd; Incubation period is short, and the process of pathological changes is directly related with the dosage of infection, can cause liver hepatic necrosis, fatty distortion, liver cirrhosis and hepatocarcinoma in various degree.
Along with the development of society, the people of hepatic injury is more and more, and the health food of hepatic injury auxiliary protection is had very big adaptation population.Successive dynasties medical book Huangdi's Internal Classics, Shennong's Herbal, " prescriptions worth thousand gold ", Compendium of Material Medica, " Mingyi Bielu " etc. have all been put down in writing the Chinese herbal medicine of hepatoprotective.Modern age more occurred various hepatoprotective prescriptions, but manufacture method adopts the traditional Chinese medicines decocting method more, effective ingredient is not clear; The mechanism of action is unclear; Do not provide enough pharmacology and pathology to explain, animal or human's body test of being correlated with simultaneously, the toxicity hazardness is indeterminate.
Thereby that develops determined curative effect has the medicine of auxiliary protection function to become the task of top priority of control liver damage disease to chemical liver injury.
Summary of the invention
The purpose of this invention is to provide a kind of compositions that chemical liver injury is had auxiliary protection function.
Of the present invention a kind ofly have the compositions of auxiliary protection function to chemical liver injury, comprises the raw material of following weight portion: Fructus Schisandrae Chinensis 1-10 part, Radix Puerariae 1-15 part, Semen Hoveniae (Fructus Hoveniae) 1-10 part, Radix Glycyrrhizae 0.1-10 part.
According to the further characteristic of compositions of the present invention, said compositions comprises the raw material of following weight portion: Fructus Schisandrae Chinensis 2-5 part, Radix Puerariae 2-6 part, Semen Hoveniae (Fructus Hoveniae) 2-5 part, Radix Glycyrrhizae 0.5-4 part.
According to the further characteristic of compositions of the present invention, described compositions is processed following peroral dosage form: tablet, granule, capsule, pill, powder etc. or oral liquid.
The present invention also provides according to preparation of compositions method of the present invention, may further comprise the steps: Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are mixed, add 6-12 and doubly measure the 50-95% ethanol extraction 1-4 time; Each 1-3 hour; Extracting solution merges, and filters decompression filtrate recycling ethanol; Be concentrated in right amount, add or do not add adjuvant and process acceptable preparation on the pharmaceutics by common process.
Alternative or alternatively; Preparation of compositions method of the present invention can may further comprise the steps: Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are mixed, add 6-12 times of water gaging and extract 1-4 time, each 1-3 hour; Extracting solution merges; Filter, filtrate decompression is concentrated in right amount, adds or do not add adjuvant to process acceptable preparation on the pharmaceutics by common process.
Alternative or alternatively; Preparation of compositions method of the present invention can may further comprise the steps: said materials of weight proportions is added 6-12 respectively doubly measure the 50-95% ethanol extraction 1-4 time, each 1-3 hour, extracting solution merged; Filter; Decompression filtrate recycling ethanol is concentrated in right amount, adds or does not add adjuvant and process acceptable preparation on the pharmaceutics by common process.
Alternative or alternatively; Preparation of compositions method of the present invention can may further comprise the steps: said materials of weight proportions is added 6-12 times of water gaging respectively extract 1-4 time; Each 1-3 hour, extracting solution merged, and filtered; Filtrate decompression is concentrated in right amount, adds or do not add adjuvant to process acceptable preparation on the pharmaceutics by common process.
The present invention also provides the application that is used in preparation chemical liver injury being had the health food or the medicine of auxiliary protection function according to compositions of the present invention.
Compositions of the present invention be on the basis of existing theory of Chinese medical science, filter out chemical liver injury is had the prescription of auxiliary protection function; Through the science compatibility; Bring into play its mutual synergism; Chemical liver injury is had the compositions of auxiliary protection function through experiment confirm is a kind of, can further prepare the health food or the medicine that chemical liver injury are had auxiliary protection function.
The specific embodiment
In the compositions of the present invention, the mechanism of action of each raw material components is following:
Fructus Schisandrae Chinensis: warm in nature, sour in the mouth, sweet; Return lung, the heart, kidney channel.Restrain astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, kidney calming.Modern study has found that Fructus Schisandrae Chinensis has the significant protection effect to liver; Can reduce the serum transaminase rising effect that causes because of chemical substance; Can obviously alleviate the necrosis of lobules of liver, and prevent the exhaustion of liver glutathion inside, strengthen the speed of hepatomicrosome metabolism acetaminophen.The schisandrol extracting solution has the certain protection effect to the rat chronic hepatic injury, and cardiovascular system is had vasodilative effect, and promotes the spleen immunologic function, can resist allohisto compatibility's graft-rejection, delaying senility function simultaneously.
Radix Puerariae: cool in nature, gas is put down, sweet in the mouth, goes into spleen, stomach warp.Have effects such as heat clearing away, pathogenic fire reducing, toxin expelling, the master helps digestion, separates all poison, controls on day row.Modern study finds that Radix Puerariae has the significant protection effect to the chronic hepatic injury animal model, and its mechanism is relevant with effects such as protection liver plasma membrane, removing free radical, anti peroxidation of lipid and fibrosis.
Semen Hoveniae (Fructus Hoveniae): property is flat, and sweet in the mouth is gone into the heart, spleen channel, and the relieving restlessness of quenching the thirst, damp and hot, alcoholic intoxication disappears.Modern study shows that Semen Hoveniae (Fructus Hoveniae) has anti peroxidation of lipid and protection hepatic injury effect; Can alleviate fibrosis and hepatocellular degeneration temple and inflammatory cell infiltration, mitochondrial membrane is in time recovered, reduce reticulum dilatation; Promote cell regeneration, the incidence and development of retardance hepatic fibrosis.
Radix Glycyrrhizae: property is flat, and sweet in the mouth is returned 12 warps.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Be used for weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, the anxious pain of extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong.Research shows that Radix Glycyrrhizae has the function of antiinflammatory and the reflection of resistance attitude, therefore clinically mainly as demulcent.Radix Glycyrrhizae can also promote stomach mucus to form and secretion, prolongs the epithelial cell life-span, and anti-inflammatory activity is arranged, and is usually used in the treatment of chronic ulcer and duodenal ulcer.
When stating medicine in the use, both can adopt with the medicine that is equivalent to said weight proportion is that raw material cleans respectively, dry, pulverize, mix the granule or the powder that obtain meeting preparation requirement granularity and directly take.Also can adopt with the medicine that is equivalent to said weight proportion relation is that raw material adds pharmaceutic adjuvant through suitably handling the back, is made into various preparations as required.Be prepared into by the above-mentioned raw materials medicine in the process of preparation, the above-mentioned raw materials medicine can adopt following method to handle: add water or Different concentrations of alcohol respectively and extract, the extracting solution concentrate drying gets crude extract; Or adopt further that alcohol deposition method, water returns molten method, organic solvent extractionprocess, flocculent precipitation, column chromatography one or more unite use carry out after suitably refining extract; Adoptable concrete operations and/or method for using when above-mentioned effective medicinal ingredient is extracted; Both can be that ingredient with described each proportional quantities is a raw material; Extract decomposite mode behind its effective medicinal ingredient respectively, also can adopt each medicine material by said proportional quantities to mix after the mode of co-extracted again.Adopt different extraction means, equipment and actual conditionses such as required desirable or best extraction temperature, solvent load, extraction time, extraction time when extracting, then can be according to practical situation through test by screening with find.
In following examples, the source of the raw material of employing is:
Fructus Schisandrae Chinensis: for buying gained through approach such as medical material market, operative norm is " Chinese Pharmacopoeia 2010 version one one ".
Radix Puerariae: for buying gained through approach such as medical material market, operative norm is " Chinese Pharmacopoeia 2010 version one one ".
Semen Hoveniae (Fructus Hoveniae): for buying gained through approach such as medical material markets, operative norm is " first of the Sanitation Ministry medicine standard (Chinese crude drug) ".
Radix Glycyrrhizae: for buying gained through approach such as medical material market, operative norm is " Chinese Pharmacopoeia 2010 version one one ".
Embodiment one:
Raw material: Fructus Schisandrae Chinensis 30g, Radix Puerariae 50g, Semen Hoveniae (Fructus Hoveniae) 30g, Radix Glycyrrhizae 20g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are pulverized the back add 10 times of amount 70% ethanol extractions 3 times with Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae, each 2 hours, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder; Add an amount of mannitol, Aspartane, mixing, 85% alcohol granulation; Drying, granulate promptly gets granule.
Embodiment two:
Raw material: Fructus Schisandrae Chinensis 50g, Radix Puerariae 50g, Semen Hoveniae (Fructus Hoveniae) 50g, Radix Glycyrrhizae 5g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are pulverized the back add 6 times of amount 95% ethanol extractions 4 times with Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae, each 1 hour, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder; Add an amount of dextrin, sucrose, mixing, 85% alcohol granulation; Drying, granulate promptly gets granule.
Embodiment three:
Raw material: Fructus Schisandrae Chinensis 20g, Radix Puerariae 60g, Semen Hoveniae (Fructus Hoveniae) 20g, Radix Glycyrrhizae 40g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are pulverized the back add 12 times of amount 50% ethanol extractions 1 time with Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae, each 3 hours, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder; Add appropriate amount of auxiliary materials, mixing, 85% alcohol granulation; Drying, granulate promptly gets granule.
Embodiment four:
Raw material: Fructus Schisandrae Chinensis 100g, Radix Puerariae 150g, Semen Hoveniae (Fructus Hoveniae) 100g, Radix Glycyrrhizae 100g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 8 times of amount 60% ethanol extractions 2 times, and each 2 hours, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder, mixing; 85% alcohol granulation, drying, granulate promptly gets granule.
Embodiment five:
Raw material: Fructus Schisandrae Chinensis 10g, Radix Puerariae 150g, Semen Hoveniae (Fructus Hoveniae) 10g, Radix Glycyrrhizae 100g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 10 times of amount 80% ethanol extractions 3 times, and each 1 hour, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder, and it is an amount of to add carboxymethyl starch sodium, micropowder silica gel, microcrystalline Cellulose, polyvinylpolypyrrolidone; Mixing is granulated, and drying adds magnesium stearate; Mixing, tabletting, coating or coating not promptly get tablet.
Embodiment six:
Raw material: Fructus Schisandrae Chinensis 40g, Radix Puerariae 50g, Semen Hoveniae (Fructus Hoveniae) 40g, Radix Glycyrrhizae 30g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 7 times of amount 90% ethanol extractions 2 times, and each 2 hours, extracting solution merged, filter, and decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder, granulates, drying, encapsulated, promptly get capsule.
Embodiment seven:
Raw material: Fructus Schisandrae Chinensis 25g, Radix Puerariae 35g, Semen Hoveniae (Fructus Hoveniae) 30g, Radix Glycyrrhizae 25g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion directly are ground into fine powder, and mixing adds right amount of auxiliary materials, and mixing is processed pill.
Embodiment eight:
Raw material: Fructus Schisandrae Chinensis 45g, Radix Puerariae 55g, Semen Hoveniae (Fructus Hoveniae) 25g, Radix Glycyrrhizae 15g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 9 times of amount 60% ethanol extractions 3 times, and each 1.5 hours, extracting solution merged; Filter, decompression filtrate recycling ethanol suitably concentrates; Add an amount of suspensoid, flavoring agent, antiseptic, mixing adds the water standardize solution; Filter, packing promptly gets oral liquid.
Embodiment nine:
Raw material: Fructus Schisandrae Chinensis 20g, Radix Puerariae 20g, Semen Hoveniae (Fructus Hoveniae) 20g, Radix Glycyrrhizae 5g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 6 times of water gagings extractions 2 times respectively, and each 2 hours, extracting solution merged, and filtered, and filtrate decompression is dry, is ground into fine powder, processes powder.
Embodiment ten:
Raw material: Fructus Schisandrae Chinensis 15g, Radix Puerariae 15g, Semen Hoveniae (Fructus Hoveniae) 35g, Radix Glycyrrhizae 25g
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are added 11 times of amount 80% ethanol extractions 2 times respectively, and each 3 hours, extracting solution merged, and filtered; Decompression filtrate recycling ethanol, drying under reduced pressure is ground into fine powder, with 85% alcohol granulation; Tabletting, coating or coating not promptly get tablet.
Embodiment 11:
Raw material: Fructus Schisandrae Chinensis 30g, Radix Puerariae 48g, Semen Hoveniae (Fructus Hoveniae) 30g, Radix Glycyrrhizae 20g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are pulverized the back add 10 times of water gagings extractions 3 times with Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae, each 2 hours, extracting solution merged; Filter, filtrate decompression is dry, is ground into fine powder; Add an amount of mannitol, Aspartane, mixing, 85% alcohol granulation; Drying, granulate promptly gets granule.
Embodiment 12:
Raw material: Fructus Schisandrae Chinensis 50g, Radix Puerariae 22g, Semen Hoveniae (Fructus Hoveniae) 50g, Radix Glycyrrhizae 5g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are pulverized the back add 6 times of water gagings extractions 4 times with Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae, each 1 hour, extracting solution merged, and filtered; Filtrate decompression is dry, is ground into fine powder, adds an amount of dextrin, sucrose, mixing; 85% alcohol granulation, drying, granulate promptly gets granule.
Embodiment 13:
Raw material: Fructus Schisandrae Chinensis 50g, Radix Puerariae 60g, Semen Hoveniae (Fructus Hoveniae) 50g, Radix Glycyrrhizae 40g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are added 10 times of water gagings extractions 3 times, and each 1 hour, extracting solution merged, and filtered the dry thick paste 1 that gets of filtrate decompression; Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae add 12 times of amount 50% ethanol extractions 1 time, and each 3 hours, extracting solution merged, and filtered decompression filtrate recycling ethanol; Drying under reduced pressure gets thick paste 2, and thick paste 1 mixes with thick paste 2, and drying is ground into fine powder, adds appropriate amount of auxiliary materials; Mixing, 85% alcohol granulation, drying, granulate promptly gets granule.
Embodiment 14:
Raw material: Fructus Schisandrae Chinensis 32g, Radix Puerariae 22g, Semen Hoveniae (Fructus Hoveniae) 22g, Radix Glycyrrhizae 10g
Fructus Schisandrae Chinensis, the Radix Puerariae of said weight proportion are added 8 times of amount 60% ethanol extractions 3 times respectively, and each 2 hours, extracting solution filtered, and decompression filtrate recycling ethanol, drying under reduced pressure get thick paste 1, thick paste 2 respectively; Semen Hoveniae (Fructus Hoveniae), Radix Glycyrrhizae add 10 times of water gagings respectively extracts 2 times, and each 1 hour, extracting liquid filtering, the filtrate decompression drying gets thick paste 3, thick paste 4 respectively; Thick paste 1, thick paste 2 thick pastes 3, thick paste 4 mix, and drying is ground into fine powder, with 85% alcohol granulation; Tabletting, coating or coating not promptly get tablet.
Below be that foregoing of the present invention is done further to specify for example, but should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to above giving an example, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
The active constituents of medicine of the present invention that makes can directly be used as medicine and take or add that acceptable auxiliary is prepared into required preparation by common process on the pharmaceutics.Like the oral drugs of solid preparation forms such as tablet (dispersible tablet, effervescent tablet, oral cavity disintegration tablet, buccal tablet, chewable tablet, effervescent tablet) commonly used, capsule (hard capsule, soft capsule), granule, pill (drop pill), powder, also can process the oral drugs of liquid preparation forms such as syrup, oral liquid
Adjuvant described here can be different according to different preparations, like diluent commonly used in solid preparations such as tablet, capsule, granule, disintegrating agent, excipient, binding agent, lubricant, surfactant, filler etc.; The surfactant of in liquid preparation forms such as syrup, oral liquid, using always, diluent, antiseptic, stabilizing agent, correctives, thickening agent, fluidizer etc.
Its adjuvant commonly used such as starch; Lactose; Dextrin; Icing Sugar; Microcrystalline Cellulose; Mannitol; Xylitol; Polyethylene Glycol; Calcium sulfate; Calcium hydrogen phosphate; Calcium carbonate; Modified starch; Sorbitol; Polyvidon; Heavy Magnesium Carbonate; Sodium carboxymethyl cellulose; Hydroxypropyl methylcellulose; Methylcellulose; Ethyl cellulose; Carboxymethylstach sodium; Hydroxypropyl cellulose; 30 POVIDONE K 30 BP/USP 30; Kaolin; Pregelatinized Starch; Magnesium stearate; Pulvis Talci; Micropowder silica gel; Stevioside; Betanin; Aspartame; Glycyrrhizin; Saccharin sodium; Citric acid; Sodium bicarbonate; Sodium carbonate; Carrageenan; Agar; Gelatin; Sodium alginate; Xanthan gum; Guar gum; Tragcanth; Arabic gum; Locust bean gum; POLY-karaya; Stearic acid; Glyceryl monostearate; Polyacrylamide; Cross linked sodium polyacrylate; Polyvinyl alcohol; Carbomer; Sorbic acid; Potassium sorbate; Ethyl hydroxybenzoate; Benzyl alcohol; Nipalgin; Thimerosal; Dimethyl sulfoxide; Azone; Triethanolamine; Sodium hydroxide; Glycerol; Propylene glycol; BHT; BHA; Sodium lauryl sulphate; Tweens; Spans etc.
Further set forth the beneficial effect of compositions according to the invention through following experiment:
One, present composition safety evaluatio test
1, acute toxicity test: to the acute oral LD of male and female mice
50All, belong to nontoxic level thing greater than 100000mg/kg.
2, the micronucleus test result is negative, shows that it does not have the property of bringing out to the mouse bone marrow cells chromosomal aberration.
3, sperm malformation test result is negative, shows that it is not to there is genotoxicity in vivo.
4, the Salmonella reversion test result is negative, shows that it does not have potential carcinogenecity.
5,30 days feeding trial results show that the experimental animal growing state is good, hematology, biochemical analysis, and main dirty soma learns check result and compares with matched group, and all no significant difference has further confirmed its safety.
Two, the present composition has carried out " to the auxiliary protection function of chemical liver injury " animal functional evaluation test according to " the health food function assessment assessment process and the method for inspection " (version in 2003), and method and result are following:
1, trial drug
Drug group: the embodiment of the invention 1 prepares (being divided into high, medium and low dose groups); The normal control group; Model control group.
2, experimental animal
50 of healthy mices, body weight 18-22g.Provide by Traditional Chinese Medicine University Of Guangzhou zoopery center.
3, test method
50 mices are divided into 5 groups at random; Be normal control group, liver injury model matched group, medicine high dose group of the present invention, middle dose groups, dose groups; Each dose groups is irritated stomach according to corresponding dosage; Normal control group and model control group are drunk distilled water, and continuous 30 days, the 30th day each dose groups and model group were used CCl
4Contamination, dosage 24mg/kg, lumbar injection.The 31st day, five groups of mices are plucked eyeball and get blood, and were centrifugal, get serum, measured biochemical indicator: serum glutamic pyruvic transminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), and get liver and carry out histopathologic examination.Result of the test is used the Spss software statistics.
4, result of the test
(1) biochemistry detection result
Table 1 present composition is to the influence of mice biochemical indicator
| Group | Number of animals (only) | ALT（IU/L） | AST（IU/L） |
| The normal control group | 10 | 35±10* | 80±5* |
| Model control group | 10 | 592±105 | 684±37 |
| Low dose group | 10 | 444±70 | 620±35 |
| Middle dose groups | 10 | 360±38* | 550±24* |
| High dose group | 10 | 180±34* | 185±22* |
Compare *: P＜0.05 with model control group
Can know that by table 1 ALT of normal control group, AST content are starkly lower than the carbon tetrachloride model group, have significant difference, the model establishment is described; The ALT of the middle and high dose groups of the present composition, AST content obviously reduce, and compare with model control group, all have significant difference (P＜0.05).The prompting present composition has the effect of auxiliary protection to chemical liver injury.
(2) liver histopathology inspection total mark
Table 2 liver histopathology inspection integration
Compare *: P＜0.05 with model control group
Can know that by table 2 the pathology integration of normal control group is starkly lower than model control group, has significant difference, the model establishment is described.The middle and high dose groups of the present composition is compared with model control group, and the pathology integration obviously descends, and has significant difference (P＜0.05).The prompting present composition has the effect of auxiliary protection to chemical liver injury.
Claims (9)
1. one kind has the compositions of auxiliary protection function to chemical liver injury, it is characterized in that said compositions comprises the raw material of following weight portion: Fructus Schisandrae Chinensis 1-10 part, Radix Puerariae 1-15 part, Semen Hoveniae (Fructus Hoveniae) 1-10 part, Radix Glycyrrhizae 0.1-10 part.
2. compositions according to claim 1 is characterized in that, said compositions comprises the raw material of following weight portion: Fructus Schisandrae Chinensis 2-5 part, Radix Puerariae 2-6 part, Semen Hoveniae (Fructus Hoveniae) 2-5 part, Radix Glycyrrhizae 0.5-4 part.
3. compositions according to claim 1 and 2 is characterized in that, described compositions is processed following peroral dosage form: tablet, granule, capsule, pill, powder or oral liquid.
4. preparation of compositions method according to claim 1 and 2 is characterized in that, may further comprise the steps:
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are mixed; Add 6-12 and doubly measure the 50-95% ethanol extraction 1-4 time, each 1-3 hour, extracting solution merged; Filter; Decompression filtrate recycling ethanol is concentrated in right amount, adds or does not add adjuvant and process acceptable preparation on the pharmaceutics by common process.
5. preparation of compositions method according to claim 1 and 2 is characterized in that, may further comprise the steps:
Fructus Schisandrae Chinensis, Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), the Radix Glycyrrhizae of said weight proportion are mixed, add 6-12 times of water gaging and extract 1-4 time, each 1-3 hour; Extracting solution merges; Filter, filtrate decompression is concentrated in right amount, adds or do not add adjuvant to process acceptable preparation on the pharmaceutics by common process.
6. preparation of compositions method according to claim 1 and 2 is characterized in that, may further comprise the steps:
Said materials of weight proportions is added 6-12 respectively doubly measure the 50-95% ethanol extraction 1-4 time, each 1-3 hour, extracting solution merged; Filter; Decompression filtrate recycling ethanol is concentrated in right amount, adds or does not add adjuvant and process acceptable preparation on the pharmaceutics by common process.
7. preparation of compositions method according to claim 1 and 2 is characterized in that, may further comprise the steps:
Said materials of weight proportions is added 6-12 times of water gaging respectively extract 1-4 time, each 1-3 hour, extracting solution merged, and filtered, and filtrate decompression is concentrated in right amount, adds or do not add adjuvant to process acceptable preparation on the pharmaceutics by common process.
8. compositions according to claim 1 and 2 is used in preparation chemical liver injury being had the application of the health food of auxiliary protection function.
9. compositions according to claim 1 and 2 is used in preparation chemical liver injury being had the application of the medicine of auxiliary protection function.
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| CN103127305A (en) * | 2013-03-13 | 2013-06-05 | 南京中医药大学 | Traditional Chinese medicine preparation with auxiliary protection function on alcoholic liver injury and preparation method thereof |
| CN103330198A (en) * | 2013-06-19 | 2013-10-02 | 江西安正利康生命科技有限公司 | Preparation method of health care capsule used for improving chemical liver injury |
| CN104172159A (en) * | 2014-07-24 | 2014-12-03 | 北京中泰天和科技有限公司 | Composition with auxiliary protection effect on alcoholic liver injury and preparation method thereof |
| CN104547215A (en) * | 2014-11-29 | 2015-04-29 | 张莘蔓 | Traditional Chinese medicine composition containing centella asiatica |
| CN105707885A (en) * | 2016-02-03 | 2016-06-29 | 聊城大学 | Health product with liver repairing function and preparation method |
| CN105796753A (en) * | 2016-04-21 | 2016-07-27 | 广西嘉进药业股份有限公司 | Hepatoprotection traditional Chinese medicine composition and preparation method thereof |
| CN105832877A (en) * | 2016-06-01 | 2016-08-10 | 谢晓亮 | Health-care product with liver-protecting effect and preparation method and application thereof |
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| CN114027503A (en) * | 2021-11-29 | 2022-02-11 | 乐泰药业有限公司 | Composition with auxiliary protection function on chemical liver injury and quality evaluation method thereof |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1371746A (en) * | 2002-03-26 | 2002-10-02 | 北京采瑞医药有限公司 | Chinese medicine composition with functions of regulating blood-liquid and curing chemical liver injury |
| CN1706406A (en) * | 2004-06-09 | 2005-12-14 | 中国科学院大连化学物理研究所 | Health food for regulating blood fat and preventing liver against chemical damage and its prepn |
| KR20090024891A (en) * | 2007-09-05 | 2009-03-10 | 경상대학교산학협력단 | Preparation of functional hydrolysates from oyster using transglutaminase |
| CN101513463A (en) * | 2009-03-27 | 2009-08-26 | 武汉大学 | Chinese medicine composition for relieving alcoholism and protecting liver and preparation method thereof |
| KR20100064816A (en) * | 2008-12-05 | 2010-06-15 | 동화약품주식회사 | The funtional foods comprising the extracts from eugenia caryophyllata for the improvements and prevention of the symptoms in the liver injury induced by the treatment of hyperlipidemia |
| CN102150842A (en) * | 2010-12-30 | 2011-08-17 | 广东太阳神集团有限公司 | Liver-protecting and antialcoholismic oral liquid and preparation method thereof |
| CN102335379A (en) * | 2010-07-20 | 2012-02-01 | 张立中 | Traditional Chinese preparation for relieving alcoholism and protecting livers |
-
2012
- 2012-06-01 CN CN2012101769322A patent/CN102670763A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1371746A (en) * | 2002-03-26 | 2002-10-02 | 北京采瑞医药有限公司 | Chinese medicine composition with functions of regulating blood-liquid and curing chemical liver injury |
| CN1706406A (en) * | 2004-06-09 | 2005-12-14 | 中国科学院大连化学物理研究所 | Health food for regulating blood fat and preventing liver against chemical damage and its prepn |
| KR20090024891A (en) * | 2007-09-05 | 2009-03-10 | 경상대학교산학협력단 | Preparation of functional hydrolysates from oyster using transglutaminase |
| KR20100064816A (en) * | 2008-12-05 | 2010-06-15 | 동화약품주식회사 | The funtional foods comprising the extracts from eugenia caryophyllata for the improvements and prevention of the symptoms in the liver injury induced by the treatment of hyperlipidemia |
| CN101513463A (en) * | 2009-03-27 | 2009-08-26 | 武汉大学 | Chinese medicine composition for relieving alcoholism and protecting liver and preparation method thereof |
| CN102335379A (en) * | 2010-07-20 | 2012-02-01 | 张立中 | Traditional Chinese preparation for relieving alcoholism and protecting livers |
| CN102150842A (en) * | 2010-12-30 | 2011-08-17 | 广东太阳神集团有限公司 | Liver-protecting and antialcoholismic oral liquid and preparation method thereof |
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| CN103127305B (en) * | 2013-03-13 | 2014-12-10 | 南京中医药大学 | Traditional Chinese medicine preparation with auxiliary protection function on alcoholic liver injury and preparation method thereof |
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| CN103330198A (en) * | 2013-06-19 | 2013-10-02 | 江西安正利康生命科技有限公司 | Preparation method of health care capsule used for improving chemical liver injury |
| CN104172159A (en) * | 2014-07-24 | 2014-12-03 | 北京中泰天和科技有限公司 | Composition with auxiliary protection effect on alcoholic liver injury and preparation method thereof |
| CN104547215A (en) * | 2014-11-29 | 2015-04-29 | 张莘蔓 | Traditional Chinese medicine composition containing centella asiatica |
| CN106177746A (en) * | 2015-05-06 | 2016-12-07 | 北京宝德润生医药科技发展有限公司 | A kind of to chemical liver injury compositions with auxiliary protection function and preparation method thereof |
| CN105707885A (en) * | 2016-02-03 | 2016-06-29 | 聊城大学 | Health product with liver repairing function and preparation method |
| CN105796753A (en) * | 2016-04-21 | 2016-07-27 | 广西嘉进药业股份有限公司 | Hepatoprotection traditional Chinese medicine composition and preparation method thereof |
| CN105832877A (en) * | 2016-06-01 | 2016-08-10 | 谢晓亮 | Health-care product with liver-protecting effect and preparation method and application thereof |
| CN105832877B (en) * | 2016-06-01 | 2019-07-16 | 河北省农林科学院经济作物研究所 | A kind of health care product and its preparation method and application with liver-protecting efficacy |
| CN106538778A (en) * | 2016-11-09 | 2017-03-29 | 辽宁佰福药业有限公司 | A kind of medicine juice Hibiscus manihot L. Antialcoholic tea and preparation method thereof |
| CN108578518A (en) * | 2018-05-28 | 2018-09-28 | 陕西科技大学 | A kind of oral disnitegration tablet and preparation method thereof for drunk crowd |
| CN114521648A (en) * | 2020-11-23 | 2022-05-24 | 辽宁医学诊疗科技研发中心有限公司 | Food composition with auxiliary protection function on chemical liver injury and preparation method thereof |
| CN114521648B (en) * | 2020-11-23 | 2024-05-14 | 辽宁健一医药科技有限责任公司 | Food composition with auxiliary protection function on chemical liver injury and preparation method thereof |
| CN114027503A (en) * | 2021-11-29 | 2022-02-11 | 乐泰药业有限公司 | Composition with auxiliary protection function on chemical liver injury and quality evaluation method thereof |
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