CN102669184A - Preparation for reducing meloidogyne and preparation method thereof - Google Patents
Preparation for reducing meloidogyne and preparation method thereof Download PDFInfo
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- CN102669184A CN102669184A CN2012100108873A CN201210010887A CN102669184A CN 102669184 A CN102669184 A CN 102669184A CN 2012100108873 A CN2012100108873 A CN 2012100108873A CN 201210010887 A CN201210010887 A CN 201210010887A CN 102669184 A CN102669184 A CN 102669184A
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- knot nematode
- trichoderma harzianum
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Abstract
The invention relates to a preparation for reducing meloidogyne and a preparation method thereof. Each liter of preparation contains 25 to 35 milligrams of trichoderma harzianum spore powder, 2 to 5 grams of maltose, 0.05 to 0.07 gram of ferrous sulfate, 0.6 to 0.8 gram of magnesium sulfate, 0.6 to 0.7 gram of composite amino acid, 0.04 to 0.06 gram of boric acid, 8 milliliters of ethoxy trisiloxane, 1.0 to 2.0 milliliters of Tween-80, and the balance of water. The preparation is easy to prepare, has the advantages of convenience in use and low cost, and does not have side effect. 40 to 60 percent of root knots of a plant can be remarkably reduced; and after the preparation is used, the resistance of the plant can be remarkably improved, and the consumption of pesticide is reduced. The preparation is suitable for crops such as vegetables, fruit trees, flowers and tea leaves, and is suitable to be popularized and applied in vast rural areas in China.
Description
Technical field
The present invention relates to a kind of preparation that root-knot nematode takes place that is used to reduce, also relate to a kind of preparation method of preparation simultaneously.
Background technology
Root-knot nematode (Meloidogyne spp.) is to parasitize crop root, one type of phytopathy original that the serious harm world agriculture is produced.Its host plant surpasses 3000 kinds, belongs to 114 sections, comprises paddy rice, peanut, tobacco, tealeaves, fruit tree, vegetables, flowers, medicinal material etc., and is wherein particularly serious with hazard of plant such as Solanaceae, Curcurbitaceae, Cruciferae.Root knot nematode disease main harm root forms the root knot.After the crop root morbidity, the basic function of root is benefited from, and institutional framework is destroyed; Directly influence the cauline leaf growth of overground part, the blade yellow, fine day before midday after; Particularly under the dry climate condition, blade is wilted, like the shape down water deficient cause. before the diseased plant growth retardation not.
Also do not have the very means of prevention of special efficacy for root-knot nematode at present, remove cultural controls such as breeding for disease resistance, rice field-upland field rotation, outside the physical controls such as the stewing canopy of high temperature, mainly still prevent and treat through chemical pesticide.The chemical pesticide of control root-knot nematode belongs to the single agent or the complex preparation of organic phosphor or carbamates more, and toxicity is bigger, and excessive use has produced problems such as environmental pollution is serious, nematode is prone to develop immunity to drugs.
Summary of the invention
The object of the present invention is to provide a kind of preparation that root-knot nematode is taken place that is used to reduce, quicken to kill the content of nematode from root, thereby improve the product safety quality.
Simultaneously, the present invention also aims to provide a kind of preparation method who is used to reduce the preparation that root-knot nematode takes place.
To achieve these goals, technical scheme of the present invention has adopted a kind of being used to reduce the preparation that root-knot nematode is taken place: contain in every liter of preparation: Trichoderma harzianum conidial powder 25～35mg, maltose 2～5g; Ferrous sulfate: 0.05～0.07g, magnesium sulfate: 0.6～0.8g, compound amino acid 0.6～0.7g; Boric acid 0.04～0.06g; Trisiloxane ethoxylate 8ml, Tween 80 1.0～2.0ml, surplus is a water.
Described Trichoderma harzianum conidial powder is inoculated on the conidial powder medium by trichoderma harzianum strain TH-18 and cultivates, and dries, and pulverizes the back and obtains.
Described trichoderma harzianum strain is TH-18; Latin name is: Trichoderma harzianum; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 8th, 2011; The preservation accession designation number is CGMCC No.5547, the preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
The mass concentration of said Tween 80 is 98%.
Described conidial powder medium consists of: wheat bran 400-600g, rapeseed cake 300-500g, corn stalk powder 80-100g g, potassium dihydrogen phosphate 0.9-1.0g, potassium nitrate 0.8-1.0g, sodium tetraborate 0.8-1.0g; Add water 1000ml, add Trichoderma silk piece (Φ=0.5mm) 100 (raw material need pulverize granularity be below the 2.0mm).
The granularity of medium is below the 2.5mm.
Further, technical scheme of the present invention has adopted a kind of preparation method who is used to reduce the preparation that root-knot nematode takes place, and may further comprise the steps:
(1) takes by weighing each component in proportion;
(2) the Trichoderma harzianum conidial powder water-soluble after, add the Tween 80 of mass concentration 98%, homogeneous mixture solotion;
(3) be mixed together the same maltose of solution, ferrous sulfate, magnesium sulfate, boric acid, trisiloxanes and the compound amino acid of step (2) configuration and water evenly and get final product.
In each raw material of the present invention, maltose is mainly Trichoderma harzianum nutrition is provided as carbon source; Ferro element is that the formation plant chlorophyll is necessary, to improve photosynthetic capacity, promotes preparation to absorb; Ferrous sulfate is low than other ferrous salt prices, can reduce cost; Magnesium salts selects magnesium sulfate to be because its hygroscopicity is strong, viscosity is big as the activator of plurality of enzymes metabolism in the plant corpus; Compound amino acid quickens from the running speed of plant root to other positions as effective plant growth regulator; Improve crops resistance simultaneously, can significantly improve the anti-poisoning ability of crop after being absorbed by crop.Compound amino acid total amino acid content of the present invention>=42%, biologically active N>=16%, organic acid>=30%, moisture≤3%, pH 6～7, preferably available from " all prosperous " amino acid of all emerging means of agricultural production in Wuhan Co., Ltd; Trisiloxane ethoxylate has good plate-out ability, infiltrative organic silicon surfactant, it can let the agricultural chemicals of using bring into play better effect, sprawl more easily, absorb faster; Tween 80 has emulsification, diffusion, solubilising, stabilization as surfactant, considers during configuration that mass concentration is that 98% Tween 80 valency is low and is prone to purchase, and therefore can select but is not limited only to select 98% Tween 80.
Preparation of the present invention is through promoting the synthetic of reduced glutathione, improves resistance enzyme GST, GR, POD vigor and activates the rise of resistance related gene (WRKY6, MYB, GST2, PR-1, PAL and GLU) and effectively reduce root-knot nematode and take place.Preparation of the present invention is applicable to crops such as vegetables, fruit tree, flowers, tealeaves, is adapted at the vast rural area of China and applies.To plant nematode, playing good effect aspect the generation that reduces Meloidogyne incognita, aphelenchoides besseyi and soybean SCN especially.
Method for using:
Get preparation 10～20mL of the present invention, add clear water 1L, after mixing, water in the plant rhizosphere soil layer, 50-100mL is watered in every strain.Use generally in the morning or carry out at dusk.Test shows, just can significantly reduce root-knot nematode after this preparation waters 5～6 times and take place.
Preparation of the present invention has following advantage with respect to prior art:
Preparation of the present invention has the advantage that manufacturing is simple, easy to use, cost is low and have no side effect.Can significantly reduce plant roots footing 40%～60%, simultaneously, also can significantly improve the resistance of plant after the use, reduce the usage amount of agricultural chemicals.Be applicable to crops such as vegetables, fruit tree, flowers, tealeaves, be adapted at the vast rural area of China and apply.
Embodiment
Embodiment 1
The preparation that the reduction root-knot nematode of present embodiment is taken place, form as follows according to every 1L meter:
Trichoderma harzianum conidial powder: 30mg
Maltose: 5g
Ferrous sulfate: 0.07g
Magnesium sulfate: 0.8g
Compound amino acid: 0.5g
Boric acid: 0.05g
Trisiloxane ethoxylate: 8ml
Mass concentration is 98% Tween 80: 2.0ml
Surplus is a water.
Compound method: take by weighing each component according to the above ratio, earlier the Trichoderma harzianum conidial powder water-soluble after, the adding mass concentration is 98% Tween 80, homogeneous mixture solotion; Be mixed together the same ferrous sulfate of this solution, magnesium sulfate, maltose, boric acid, trisiloxanes and compound amino acid and water evenly again.
The Trichoderma harzianum conidial powder is inoculated on the conidial powder medium by trichoderma harzianum strain TH-18 and cultivates, and dries, and pulverizes the back and obtains; Wherein, Trichoderma harzianum strain is TH-18, is preserved in Chinese common micro-organisms culture presevation administrative center, and the preservation accession designation number is CGMCC No.5547.
The result of use of preparation of the present invention: in the greenhouse that has root-knot nematode to take place, bloom the phase of bearing fruit from watermelon, get this preparation 200mL, add and water in watermelon rhizosphere soil layer after clear water 10L mixes, 50-100mL is watered in every strain.Watering clear water simultaneously compares.Thereafter, every separated 5d waters once continuous pouring 8 times.Measure its root footing order, nematode density, control efficiency.Reduce have an effect effect and reference examples comparative result of root-knot nematode and see table 1, table 2.Control efficiency (%)=(the routine root footing of reference examples root footing-invention)/reference examples root footing * 100%.
Embodiment 2
The preparation that the reduction root-knot nematode of present embodiment is taken place, form as follows according to every 1L meter:
Trichoderma harzianum conidial powder: 25mg
Maltose: 2g
Ferrous sulfate: 0.05g
Magnesium sulfate: 0.6g
Compound amino acid: 0.6g
Boric acid: 0.04g
Trisiloxane ethoxylate: 8ml
Mass concentration is 98% Tween 80: 1.0ml
Surplus is a water.
The result of use of preparation of the present invention: in the greenhouse that has root-knot nematode to take place, bloom the phase of bearing fruit from muskmelon, get this preparation 200mL, add and water in muskmelon rhizosphere soil layer after clear water 10L mixes, 50-100mL is watered in every strain.Watering clear water simultaneously compares.Thereafter, every separated 5d waters once continuous pouring 8 times.Measure its root footing order, nematode density, control efficiency.Reduce have an effect effect and reference examples comparative result of root-knot nematode and see table 1, table 2.Control efficiency (%)=(the routine root footing of reference examples root footing-invention)/reference examples root footing * 100%.
Embodiment 3
The preparation that the reduction root-knot nematode of present embodiment is taken place, form as follows according to every 1L meter:
Trichoderma harzianum conidial powder: 35mg
Maltose: 3g
Ferrous sulfate: 0.06g
Magnesium sulfate: 0.7g
Compound amino acid: 0.7g
Boric acid: 0.06g
Trisiloxane ethoxylate: 8ml
Mass concentration is 98% Tween 80: 1.5ml
Surplus is a water.
The result of use of preparation of the present invention: in the greenhouse that has root-knot nematode to take place, bloom the phase of bearing fruit from cucumber, get this preparation 200mL, add and water in cucumber rhizosphere soil layer after clear water 10L mixes, 50-100mL is watered in every strain.Watering clear water simultaneously compares.Thereafter, every separated 5d waters once continuous pouring 6 times.Measure its root footing order, nematode density, control efficiency.Reduce have an effect effect and reference examples comparative result of root-knot nematode and see table 1, table 2.Control efficiency (%)=(the routine root footing of reference examples root footing-invention)/reference examples root footing * 100%.
Table 1 preparation of the present invention reduces root-knot nematode effect takes place
Table 2 biomass statistics
Claims (7)
1. one kind is used to reduce the preparation that root-knot nematode is taken place, and it is characterized in that: contain in every liter of preparation: Trichoderma harzianum conidial powder 25～35mg, maltose 2～5g; Ferrous sulfate: 0.05～0.07g, magnesium sulfate: 0.6～0.8g, compound amino acid 0.6～0.7g; Boric acid 0.04～0.06g; Trisiloxane ethoxylate 8ml, Tween 80 1.0～2.0ml, surplus is a water.
2. 1 described being used to reduced the preparation that root-knot nematode is taken place as requested, and it is characterized in that: described Trichoderma harzianum conidial powder is inoculated on the conidial powder medium by trichoderma harzianum strain TH-18 and cultivates, and dries, and pulverized the back and obtained.
3. 2 describedly are used to reduce the preparations that root-knot nematode is taken place as requested, it is characterized in that: described trichoderma harzianum strain is TH-18, is preserved in Chinese common micro-organisms culture presevation administrative center, and the preservation accession designation number is CGMCC No.5547.
4. 1 described being used to reduced the preparation that root-knot nematode is taken place as requested, and it is characterized in that: the mass concentration of said Tween 80 is 98%.
5. 2 describedly be used to reduce the preparations that root-knot nematode is taken place as requested; It is characterized in that: described conidial powder medium consists of: wheat bran 400-600g, rapeseed cake 300-500g, corn stalk powder 80-100g, potassium dihydrogen phosphate 0.9-1.0g, potassium nitrate 0.8-1.0g, sodium tetraborate 0.8-1.0g; Add water 1000ml, add Trichoderma silk piece (Φ=0.5mm) 100 (raw material need pulverize granularity be below the 2.0mm).
6. 1 described being used to reduced the preparation that root-knot nematode is taken place as requested, and it is characterized in that: the granularity of medium is below the 2.5mm.
7. preparation method who is used to reduce the preparation that root-knot nematode takes place is characterized in that: may further comprise the steps:
(1) takes by weighing each component in proportion;
(2) the Trichoderma harzianum conidial powder water-soluble after, add the Tween 80 of mass concentration 98%, homogeneous mixture solotion;
(3) be mixed together the same maltose of solution, ferrous sulfate, magnesium sulfate, boric acid, trisiloxanes and the compound amino acid of step (2) configuration and water evenly and get final product.
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| CN201210010887.3A CN102669184B (en) | 2012-01-15 | 2012-01-15 | Preparation for reducing meloidogyne and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103484380A (en) * | 2013-08-26 | 2014-01-01 | 山东省科学院中日友好生物技术研究中心 | Parasitism effect of trichoderma T11-W on root-knot nematode egg and application of trichoderma T11-W |
| CN104496643A (en) * | 2014-11-29 | 2015-04-08 | 张旭 | Organic base fertilizer for planting of tea tree |
| CN105145642A (en) * | 2015-10-26 | 2015-12-16 | 广西南宁益土生物科技有限责任公司 | Nematicidal granules containing fosthiazate |
| WO2016086830A1 (en) * | 2014-12-02 | 2016-06-09 | Rotam Agrochem International Company Limited | Composition and method for treating nematodes |
| CN106348558A (en) * | 2016-09-30 | 2017-01-25 | 洛阳茂生生物技术有限公司 | Method for treating sludge of sewage plant by adopting microbial agent |
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| CN102050645A (en) * | 2009-10-27 | 2011-05-11 | 中国科学院生态环境研究中心 | Method for producing trichoderma microbial fertilizer by utilizing sweet potato starch wastewater |
| CN102177921A (en) * | 2010-10-22 | 2011-09-14 | 刘和现 | Compound biological control agent as well as preparation method and application thereof |
| CN102277302A (en) * | 2011-06-15 | 2011-12-14 | 上海交通大学 | Construction method for combining trichoderma and brassica napobrassica for absorbing farmland soil heavy metals cadmium and zinc |
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| CN102050645A (en) * | 2009-10-27 | 2011-05-11 | 中国科学院生态环境研究中心 | Method for producing trichoderma microbial fertilizer by utilizing sweet potato starch wastewater |
| CN102177921A (en) * | 2010-10-22 | 2011-09-14 | 刘和现 | Compound biological control agent as well as preparation method and application thereof |
| CN102277302A (en) * | 2011-06-15 | 2011-12-14 | 上海交通大学 | Construction method for combining trichoderma and brassica napobrassica for absorbing farmland soil heavy metals cadmium and zinc |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484380A (en) * | 2013-08-26 | 2014-01-01 | 山东省科学院中日友好生物技术研究中心 | Parasitism effect of trichoderma T11-W on root-knot nematode egg and application of trichoderma T11-W |
| CN104496643A (en) * | 2014-11-29 | 2015-04-08 | 张旭 | Organic base fertilizer for planting of tea tree |
| WO2016086830A1 (en) * | 2014-12-02 | 2016-06-09 | Rotam Agrochem International Company Limited | Composition and method for treating nematodes |
| CN106998694A (en) * | 2014-12-02 | 2017-08-01 | 江苏龙灯化学有限公司 | Handle the composition and method of nematode |
| US10631538B2 (en) | 2014-12-02 | 2020-04-28 | Jiangsu Rotam Chemistry Co., Ltd. | Composition and method for treating nematodes |
| CN105145642A (en) * | 2015-10-26 | 2015-12-16 | 广西南宁益土生物科技有限责任公司 | Nematicidal granules containing fosthiazate |
| CN105145642B (en) * | 2015-10-26 | 2018-09-25 | 东阳市特意新材料科技有限公司 | A kind of granular formulation for killing nematode containing fosthiazate |
| CN106348558A (en) * | 2016-09-30 | 2017-01-25 | 洛阳茂生生物技术有限公司 | Method for treating sludge of sewage plant by adopting microbial agent |
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Application publication date: 20120919 Assignee: Songxian golden Foping agricultural science and Technology Co Ltd Assignor: Henan University of Science and Technology Contract record no.: X2019980000413 Denomination of invention: Preparation for reducing meloidogyne and preparation method thereof Granted publication date: 20140416 License type: Exclusive License Record date: 20191101 |