CN102667473B - 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现 - Google Patents

用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现 Download PDF

Info

Publication number
CN102667473B
CN102667473B CN201080046023.6A CN201080046023A CN102667473B CN 102667473 B CN102667473 B CN 102667473B CN 201080046023 A CN201080046023 A CN 201080046023A CN 102667473 B CN102667473 B CN 102667473B
Authority
CN
China
Prior art keywords
image
images
contrast
color
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201080046023.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN102667473A (zh
Inventor
K.加沙
G.佩斯塔诺
M.奥特
A.D.纳吉
R.B.纳格勒
P.米勒
J.弗勒利希
W.戴
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of CN102667473A publication Critical patent/CN102667473A/zh
Application granted granted Critical
Publication of CN102667473B publication Critical patent/CN102667473B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201080046023.6A 2009-10-12 2010-10-07 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现 Expired - Fee Related CN102667473B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25080909P 2009-10-12 2009-10-12
US61/250809 2009-10-12
US27893609P 2009-10-13 2009-10-13
US61/278936 2009-10-13
PCT/US2010/051857 WO2011046807A2 (en) 2009-10-12 2010-10-07 Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

Publications (2)

Publication Number Publication Date
CN102667473A CN102667473A (zh) 2012-09-12
CN102667473B true CN102667473B (zh) 2016-06-08

Family

ID=43876802

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080046023.6A Expired - Fee Related CN102667473B (zh) 2009-10-12 2010-10-07 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现

Country Status (10)

Country Link
US (1) US9310302B2 (https=)
EP (1) EP2488860A4 (https=)
JP (1) JP5540102B2 (https=)
KR (1) KR101388291B1 (https=)
CN (1) CN102667473B (https=)
AU (1) AU2010307140B2 (https=)
CA (1) CA2774422C (https=)
IL (1) IL218553B (https=)
IN (1) IN2012DN02337A (https=)
WO (1) WO2011046807A2 (https=)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2652487B1 (en) * 2010-12-13 2018-02-21 Koninklijke Philips N.V. Method and device for analysing a region of interest in an object using x-rays
US8639013B2 (en) * 2011-08-17 2014-01-28 General Electric Company System and methods for generating a brightfield image using fluorescent images
JP5826561B2 (ja) * 2011-08-24 2015-12-02 オリンパス株式会社 顕微鏡システム、標本画像生成方法及びプログラム
JP5784435B2 (ja) * 2011-09-20 2015-09-24 オリンパス株式会社 画像処理装置、蛍光顕微鏡装置および画像処理プログラム
CN102426124B (zh) * 2011-11-08 2013-07-03 中国人民解放军第四军医大学 用于病理组织冰冻切片的捞片展片装置及方法
SG193046A1 (en) * 2012-02-23 2013-09-30 Histoindex Pte Ltd A digital imaging system for biopsy inspection
US9322777B2 (en) 2012-10-29 2016-04-26 Tokitae Llc Systems, devices, and methods employing angular-resolved scattering and spectrally resolved measurements for classification of objects
WO2014089494A1 (en) * 2012-12-06 2014-06-12 Clarient Diagnostic Services, Inc. Systems and methods for split screen display of a biological specimen and for capturing records thereof
RU2012156158A (ru) * 2012-12-24 2014-07-10 ЭлЭсАй Корпорейшн Генерация целевого изображения с использованием функционала на основе функций от информации из других изображений
EP2972231B1 (en) 2013-03-12 2022-09-07 Cytoviva, Inc. Three-dimensional image processing to locate nanoparticles in biolgical and nonbiological media
US9786050B2 (en) * 2013-03-15 2017-10-10 The Board Of Trustees Of The University Of Illinois Stain-free histopathology by chemical imaging
US10986997B2 (en) * 2013-12-31 2021-04-27 Memorial Sloan Kettering Cancer Center Systems, methods, and apparatus for multichannel imaging of fluorescent sources in real time
TW201546486A (zh) * 2014-06-11 2015-12-16 Univ Nat Cheng Kung 利用數位微型反射鏡元件之多光子螢光激發顯微裝置
GB2528283B (en) * 2014-07-16 2020-08-05 Barco Nv Image colour calibration with multiple colour scales
JP6531403B2 (ja) * 2015-01-27 2019-06-19 株式会社ニコン 顕微鏡
AU2016228748B2 (en) * 2015-03-11 2021-08-19 The General Hospital Corporation Plasmonic nanoparticle immunoassay method
US9953133B2 (en) 2015-06-03 2018-04-24 General Electric Company Biological data annotation and visualization
US10672505B2 (en) 2015-06-03 2020-06-02 General Electric Company Biological data annotation and visualization
CA3021538C (en) * 2015-06-11 2023-09-26 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Systems and methods for finding regions of interest in hematoxylin and eosin (h&e) stained tissue images and quantifying intratumor cellular spatial heterogeneity in multiplexed/hyperplexed fluorescence tissue images
WO2016203952A1 (ja) * 2015-06-16 2016-12-22 コニカミノルタ株式会社 病理標本、病理標本の作製方法、および蛍光画像の取得方法
JP6841609B2 (ja) 2015-07-10 2021-03-10 ３スキャン インコーポレイテッド 組織学的染色の空間的多重化
WO2017060954A1 (ja) 2015-10-05 2017-04-13 オリンパス株式会社 撮像装置
JP6703824B2 (ja) * 2015-11-30 2020-06-03 シスメックス株式会社 細胞選択方法、細胞検出方法、細胞選択装置、および細胞検出装置
JP6597316B2 (ja) * 2016-01-06 2019-10-30 コニカミノルタ株式会社 画像処理装置及びプログラム
JP6763407B2 (ja) * 2016-01-19 2020-09-30 コニカミノルタ株式会社 画像処理装置及びプログラム
WO2017136676A1 (en) 2016-02-05 2017-08-10 Nanoview Diagnostics Inc. Detection of exosomes having surface markers
MX2018009566A (es) 2016-02-08 2019-05-30 Imago Systems Inc Sistema y metodo para la visualizacion y caracterizacion de objetos en imagenes.
CN109074645B (zh) * 2016-04-06 2021-12-14 豪夫迈·罗氏有限公司 用于ihc图像分析的空间索引创建
WO2017212055A1 (en) * 2016-06-10 2017-12-14 F. Hoffmann-La Roche Ag System for bright field image simulation
EP3264741A1 (en) * 2016-06-30 2018-01-03 Thomson Licensing Plenoptic sub aperture view shuffling with improved resolution
US10324041B2 (en) 2016-12-21 2019-06-18 Abbott Japan Co., Ltd. Optical imaging system using lateral illumination for digital assays
US11047854B2 (en) 2017-02-06 2021-06-29 Abbott Japan Llc Methods for reducing noise in signal-generating digital assays
US10580130B2 (en) * 2017-03-24 2020-03-03 Curadel, LLC Tissue identification by an imaging system using color information
TWI654443B (zh) * 2017-06-05 2019-03-21 銳準醫光股份有限公司 採用進階光學干涉顯微術之光學切層裝置
EP3654020B1 (en) * 2017-07-11 2023-06-21 Hamamatsu Photonics K.K. Sample observation device and sample observation method
EP3654018B1 (en) 2017-07-11 2023-06-21 Hamamatsu Photonics K.K. Sample observation device and sample observation method
TWI659395B (zh) * 2017-07-19 2019-05-11 AcuSolutions Inc. 類ｈ＆ｅ影像之合成方法及採用該方法之光學系統
CA3076847A1 (en) * 2017-10-09 2019-04-18 Pathspot Technologies Inc. Systems and methods for detection of contaminants on surfaces
US10446113B2 (en) * 2018-01-30 2019-10-15 ForeFlight LLC Method and system for inversion of raster images
WO2019172901A1 (en) * 2018-03-07 2019-09-12 Google Llc Virtual staining for tissue slide images
TWI683283B (zh) * 2018-04-03 2020-01-21 薩摩亞商銳準醫光股份有限公司 一種生物樣本之影像合成方法及採用該方法之光學系統
JP7298993B2 (ja) 2018-04-09 2023-06-27 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
JP7007227B2 (ja) * 2018-04-09 2022-01-24 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
WO2019236655A1 (en) * 2018-06-06 2019-12-12 Trustees Of Boston University Systems and methods for imaging microwell plate samples
CN110648371B (zh) * 2018-06-27 2023-05-09 阿里巴巴集团控股有限公司 Rgb值与色号信息映射关系的确定方法及装置
US20210199582A1 (en) * 2018-06-28 2021-07-01 The Regents Of The University Of California Producing a composite image of a stained tissue sample by combining image data obtained through brightfield and fluorescence imaging modes
WO2020039520A1 (ja) * 2018-08-22 2020-02-27 オリンパス株式会社 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム
US12016690B2 (en) * 2019-02-12 2024-06-25 Purdue Research Foundation Characterization of injection-induced tissue swelling during subcutaneous injection of biologics
JP7227389B2 (ja) 2019-02-13 2023-02-21 ベンタナ メディカル システムズ， インコーポレイテッド マルチチャネル画像における自己蛍光の寄与を算出するシステムおよび方法
JP7470339B2 (ja) * 2019-03-06 2024-04-18 学校法人 埼玉医科大学 染色画像推定器学習装置、画像処理装置、染色画像推定器学習方法、画像処理方法、染色画像推定器学習プログラム、及び、画像処理プログラム
EP3709262A1 (en) 2019-03-13 2020-09-16 Koninklijke Philips N.V. Correlated image analysis for 3d biopsy
WO2021037872A1 (en) * 2019-08-28 2021-03-04 Ventana Medical Systems, Inc. Label-free assessment of biomarker expression with vibrational spectroscopy
WO2021067726A1 (en) * 2019-10-03 2021-04-08 The Regents Of The University Of California Fluorescence imitating brightfield imaging
EP3805838B1 (en) * 2019-10-10 2023-12-06 Leica Instruments (Singapore) Pte. Ltd. Microscope and related apparatuses, methods and computer programs
WO2021081253A1 (en) 2019-10-22 2021-04-29 Tempus Labs, Inc. Systems and methods for predicting therapeutic sensitivity
KR102302333B1 (ko) * 2019-11-05 2021-09-16 주식회사 토모큐브 3차원 굴절률 영상과 딥러닝을 활용한 비표지 방식의 3차원 분자 영상 생성 방법 및 장치
EP4070232A4 (en) 2019-12-05 2024-01-31 Tempus Labs, Inc. SYSTEMS AND METHODS FOR HIGH-THROUGHPUT ACTIVE SCREENING
US20230073901A1 (en) * 2020-02-01 2023-03-09 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for performing multiple-wavelength quantitative phase imaging (qpi)
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
US11561178B2 (en) * 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
CN116057359A (zh) 2020-08-03 2023-05-02 柯尼卡美能达株式会社 测光装置
JP7492881B2 (ja) * 2020-08-03 2024-05-30 株式会社日本マイクロニクス 測定システムおよび測定方法
US12251086B2 (en) 2020-09-03 2025-03-18 University Of Washington Optical fixation monitor
WO2022225845A1 (en) * 2021-04-18 2022-10-27 Mary Hitchcock Memorial Hospital, For Itself And On Behalf Of Dartmouth-Hitchcock Clinic System and method for automation of surgical pathology processes using artificial intelligence
CN115656120B (zh) * 2022-10-09 2024-07-23 中国科学院深圳先进技术研究院 一种暗场反射紫外光学显微成像方法和系统
DE102022130251A1 (de) 2022-11-16 2024-05-16 Carl Zeiss Microscopy Gmbh Mikroskopanordnung und Verfahren zur Untersuchung einer mit mehreren Farbstoffen gefärbten Probe
WO2025076554A1 (en) * 2023-10-06 2025-04-10 The Regents Of The University Of California Targeted cell profiling via in situ hybridization with mass spectrometry detection
CN117706102B (zh) * 2024-02-05 2024-04-12 遂宁市中心医院 一种肿瘤康复用光学检测装置及检测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1349633A (zh) * 1999-05-13 2002-05-15 分析科学公司 数字图像转换
CN101011259A (zh) * 2005-11-30 2007-08-08 通用电气公司 用于自动表征恶性的方法和装置
CN101061520A (zh) * 2004-11-22 2007-10-24 皇家飞利浦电子股份有限公司 改进的实时位置数据表示
CN101216601A (zh) * 2007-12-29 2008-07-09 中国科学院西安光学精密机械研究所 使用锥镜实现暗场显微及荧光显微的方法及装置
CN101351736A (zh) * 2005-12-27 2009-01-21 奥林巴斯株式会社 发光测量装置及发光测量方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288323A (en) 1979-02-05 1981-09-08 Brigante Miguel F Free flow non-corrosive water treatment device
US5162990A (en) * 1990-06-15 1992-11-10 The United States Of America As Represented By The United States Navy System and method for quantifying macrophage phagocytosis by computer image analysis
US5249077A (en) 1991-12-12 1993-09-28 Microvideo Instruments, Inc. Darkfield illuminator for a microscope slide
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
JP4348574B2 (ja) 1998-04-30 2009-10-21 株式会社ニコン 暗視野照明装置および暗視野照明方法
CA2243090A1 (en) 1998-07-10 2000-01-10 Timothy M. Richardson Inverted darkfield contrast microscope and method
US7450229B2 (en) * 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US8131053B2 (en) * 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8406498B2 (en) * 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US6403947B1 (en) 1999-03-18 2002-06-11 Cambridge Research & Instrumentation Inc. High-efficiency multiple probe imaging system
US6649351B2 (en) 1999-04-30 2003-11-18 Aclara Biosciences, Inc. Methods for detecting a plurality of analytes by mass spectrometry
WO2000068434A2 (en) 1999-05-07 2000-11-16 Yale University Multiple tag analysis
AU6754900A (en) * 1999-08-03 2001-02-19 Biophysica, Llc Spectroscopic systems and methods for detecting tissue properties
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
US20020083888A1 (en) 2000-12-28 2002-07-04 Zehnder Donald A. Flow synthesis of quantum dot nanocrystals
JP4567436B2 (ja) 2001-07-20 2010-10-20 ライフ テクノロジーズ コーポレーション 発光ナノ粒子およびそれらの調製方法
DE10144250A1 (de) 2001-08-31 2003-04-03 Fraunhofer Ges Forschung Verbesserte massenspektrometrische Analyse unter Verwendung von Nanopartikeln
JP2006014868A (ja) 2004-06-30 2006-01-19 Hamamatsu Photonics Kk リンパ節検出装置
EP1681015A1 (en) 2005-01-17 2006-07-19 Imasys SA Temperature mapping on structural data
US7524631B2 (en) * 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
US8041409B2 (en) * 2005-09-08 2011-10-18 Carestream Health, Inc. Method and apparatus for multi-modal imaging
KR100708932B1 (ko) 2005-10-06 2007-04-17 삼성전기주식회사 영상 처리장치 및 그 방법
EP1780672A1 (en) 2005-10-25 2007-05-02 Bracco Imaging, S.P.A. Method of registering images, algorithm for carrying out the method of registering images, a program for registering images using the said algorithm and a method of treating biomedical images to reduce imaging artefacts caused by object movement
WO2007062105A2 (en) 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US7551349B2 (en) 2005-12-01 2009-06-23 Auburn University High resolution optical microscope with cardioid condenser for brightfield and darkfield illumination
WO2007074929A1 (ja) 2005-12-27 2007-07-05 Olympus Corporation 生物由来の被験試料の画像を取得する装置及び方法
WO2007116509A1 (ja) * 2006-04-07 2007-10-18 Shimadzu Corporation 質量分析装置
US8126205B2 (en) 2006-09-25 2012-02-28 Cambridge Research & Instrumentation, Inc. Sample imaging and classification
US8244021B2 (en) 2006-12-20 2012-08-14 Ventana Medical Systems, Inc. Quantitative, multispectral image analysis of tissue specimens stained with quantum dots
US7327859B1 (en) * 2007-02-14 2008-02-05 Lam Ko Chau Methods and systems for automated fingerprint recognition
JP5013410B2 (ja) 2007-03-14 2012-08-29 独立行政法人産業技術総合研究所 微粒子測定方法及び装置
US7819489B2 (en) 2007-03-20 2010-10-26 Kellogg Company Concurrently printing an image on a food product and a corresponding image on packaging for the food product
US20090129650A1 (en) * 2007-11-19 2009-05-21 Carestream Health, Inc. System for presenting projection image information
JP4288323B1 (ja) * 2008-09-13 2009-07-01 独立行政法人科学技術振興機構 顕微鏡装置及びそれを用いた蛍光観察方法
US8849006B2 (en) * 2009-03-25 2014-09-30 Quorum Technologies Inc. Darkfield imaging system and methods for automated screening of cells
US8948488B2 (en) * 2009-07-31 2015-02-03 General Electric Company Methods and systems for digitally enhancing an image of a stained material
JP5338573B2 (ja) * 2009-08-31 2013-11-13 ソニー株式会社 蛍光像取得方法、蛍光像取得装置及び蛍光像取得プログラム
US8269827B2 (en) * 2009-09-29 2012-09-18 General Electric Company System and methods for mapping fluorescent images into a bright field color space
EP2601513B1 (en) * 2010-08-05 2014-05-14 Cambridge Research & Instrumentation, Inc. Enhancing visual assessment of samples

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1349633A (zh) * 1999-05-13 2002-05-15 分析科学公司 数字图像转换
CN101061520A (zh) * 2004-11-22 2007-10-24 皇家飞利浦电子股份有限公司 改进的实时位置数据表示
CN101011259A (zh) * 2005-11-30 2007-08-08 通用电气公司 用于自动表征恶性的方法和装置
CN101351736A (zh) * 2005-12-27 2009-01-21 奥林巴斯株式会社 发光测量装置及发光测量方法
CN101216601A (zh) * 2007-12-29 2008-07-09 中国科学院西安光学精密机械研究所 使用锥镜实现暗场显微及荧光显微的方法及装置

Also Published As

Publication number Publication date
EP2488860A4 (en) 2015-12-16
AU2010307140B2 (en) 2014-05-15
CA2774422A1 (en) 2011-04-21
US9310302B2 (en) 2016-04-12
AU2010307140A1 (en) 2012-04-05
CN102667473A (zh) 2012-09-12
EP2488860A2 (en) 2012-08-22
IL218553B (en) 2018-01-31
KR20120066654A (ko) 2012-06-22
JP2013507612A (ja) 2013-03-04
IL218553A0 (en) 2012-05-31
KR101388291B1 (ko) 2014-04-22
CA2774422C (en) 2017-08-29
WO2011046807A2 (en) 2011-04-21
JP5540102B2 (ja) 2014-07-02
IN2012DN02337A (https=) 2015-08-21
WO2011046807A3 (en) 2011-09-01
US20120200694A1 (en) 2012-08-09

Similar Documents

Publication Publication Date Title
CN102667473B (zh) 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现
Fereidouni et al. Microscopy with ultraviolet surface excitation for rapid slide-free histology
Morrison et al. Brightfield multiplex immunohistochemistry with multispectral imaging
US10846848B2 (en) System for bright field image simulation
EP2601513B1 (en) Enhancing visual assessment of samples
Levenson et al. Multispectral imaging in biology and medicine: slices of life
JP5631999B2 (ja) 蛍光画像を用いて明視野画像を生成するためのシステム及び方法
JP6074427B2 (ja) 蛍光画像を用いて明視野画像を生成するためのシステム及び方法
CN111433817A (zh) 生成未染色样本的虚拟染色图像
Ulaganathan et al. A clinicopathological study of various oral cancer diagnostic techniques
Wang et al. Deep learning-based virtual H& E staining from label-free autofluorescence lifetime images
Levenson et al. Modern Trends in Imaging X: Spectral Imaging in Preclinical Research and Clinical Pathology
Levenson et al. Spectral imaging in preclinical research and clinical pathology
Levenson et al. Spectral imaging for brightfield microscopy
Harmany et al. Spectral unmixing methods and tools for the detection and quantitation of collagen and other macromolecules in tissue specimens
HK1171508A (en) Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue
HK1171508B (en) Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue
Parker System of Systems Integration for Hyperspectral Imaging Microscopy
Garsha et al. Multi-modal contrast of tissue anatomy enables correlative biomarker imaging
BR112012008484A2 (pt) método, aparelho de formação de imagens, pelo menos um meio de armazenagem legível por computador e processador de imagens
HK40003631A (zh) 用於明场图像模拟的系统
Frank Measuring Drug-DNA Adducts in Individual Cells
HK40003631B (zh) 用於生成模拟数字明场ihc或ish图像的方法和图像处理系统

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1171508

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1171508

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160608

Termination date: 20211007