FR3109159B1 - Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie - Google Patents

Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie Download PDF

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FR3109159B1
FR3109159B1 FR2003596A FR2003596A FR3109159B1 FR 3109159 B1 FR3109159 B1 FR 3109159B1 FR 2003596 A FR2003596 A FR 2003596A FR 2003596 A FR2003596 A FR 2003596A FR 3109159 B1 FR3109159 B1 FR 3109159B1
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FR3109159A1 (fr
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Emmanuel Jalenques
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INTERSCIENCE
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INTERSCIENCE
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Priority to FR2003596A priority Critical patent/FR3109159B1/fr
Priority to US17/223,784 priority patent/US20210318225A1/en
Priority to CN202110369317.2A priority patent/CN113159262A/zh
Priority to GB2105015.8A priority patent/GB2595565A/en
Priority to JP2021065748A priority patent/JP2021168650A/ja
Publication of FR3109159A1 publication Critical patent/FR3109159A1/fr
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Publication of FR3109159B1 publication Critical patent/FR3109159B1/fr
Priority to US18/407,425 priority patent/US20240185392A1/en
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    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06M—COUNTING MECHANISMS; COUNTING OF OBJECTS NOT OTHERWISE PROVIDED FOR
    • G06M1/00—Design features of general application
    • G06M1/08—Design features of general application for actuating the drive
    • G06M1/10—Design features of general application for actuating the drive by electric or magnetic means
    • G06M1/101—Design features of general application for actuating the drive by electric or magnetic means by electro-optical means
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/25—Fusion techniques
    • G06F18/251—Fusion techniques of input or preprocessed data
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/70—Arrangements for image or video recognition or understanding using pattern recognition or machine learning
    • G06V10/77—Processing image or video features in feature spaces; using data integration or data reduction, e.g. principal component analysis [PCA] or independent component analysis [ICA] or self-organising maps [SOM]; Blind source separation
    • G06V10/80—Fusion, i.e. combining data from various sources at the sensor level, preprocessing level, feature extraction level or classification level
    • G06V10/803—Fusion, i.e. combining data from various sources at the sensor level, preprocessing level, feature extraction level or classification level of input or preprocessed data
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20221—Image fusion; Image merging
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

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  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Multimedia (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Computation (AREA)
  • Biochemistry (AREA)
  • Data Mining & Analysis (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Databases & Information Systems (AREA)
  • Computing Systems (AREA)
  • Software Systems (AREA)
  • Immunology (AREA)
  • Medical Informatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Sustainable Development (AREA)
  • Evolutionary Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Signal Processing (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)

Abstract

Dispositif (30) de comptage de colonies présentes dans un échantillon, doté d’un emplacement prévu pour recevoir un support agencé pour recevoir un milieu nutritif solide et ledit échantillon, comportant un premier et un deuxième organe de détection formés par un seul organe de détection (35), et dans une unité de traitement (36) est agencée pour piloter sélectivement dans une configuration en champ clair, le remplacement d’une surface diffusante sombre (24) par une surface diffusante claire (14), l’extinction de diodes électroluminescentes (23) pour configuration à champ sombre, l’allumage des diodes électroluminescentes (13) pour configuration en champ clair ; dans une configuration en champ sombre, le remplacement de la surface diffusante claire (14) par la surface diffusante sombre (24), l’extinction des diodes électroluminescentes (13) pour configuration à champ clair, l’allumage des diodes électroluminescentes (23) pour configuration à champ sombre. Figure pour l’abrégé : figure 7
FR2003596A 2020-04-09 2020-04-09 Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie Active FR3109159B1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
FR2003596A FR3109159B1 (fr) 2020-04-09 2020-04-09 Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
US17/223,784 US20210318225A1 (en) 2020-04-09 2021-04-06 Image Processing Process Applied to Colony Counters in Microbiology
CN202110369317.2A CN113159262A (zh) 2020-04-09 2021-04-06 微生物学中应用于菌落计数器的图像处理方法
GB2105015.8A GB2595565A (en) 2020-04-09 2021-04-08 Image processing method applied to colony counting in microbiology
JP2021065748A JP2021168650A (ja) 2020-04-09 2021-04-08 微生物学においてコロニーカウンターに適用される画像処理方法
US18/407,425 US20240185392A1 (en) 2020-04-09 2024-01-08 Image Processing Process Applied to Colony Counters in Microbiology

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2003596 2020-04-09
FR2003596A FR3109159B1 (fr) 2020-04-09 2020-04-09 Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie

Publications (2)

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FR3109159A1 FR3109159A1 (fr) 2021-10-15
FR3109159B1 true FR3109159B1 (fr) 2023-06-23

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FR2003596A Active FR3109159B1 (fr) 2020-04-09 2020-04-09 Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie

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US (2) US20210318225A1 (fr)
JP (1) JP2021168650A (fr)
CN (1) CN113159262A (fr)
FR (1) FR3109159B1 (fr)
GB (1) GB2595565A (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
CN113791008B (zh) * 2021-08-25 2024-03-15 安徽高哲信息技术有限公司 一种粮食不完善粒检测设备及检测方法

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JPS503355A (fr) * 1973-05-11 1975-01-14
JPS61198008A (ja) * 1985-02-28 1986-09-02 Fujitsu Ltd 画像検出装置
US5162990A (en) * 1990-06-15 1992-11-10 The United States Of America As Represented By The United States Navy System and method for quantifying macrophage phagocytosis by computer image analysis
CA2243090A1 (fr) * 1998-07-10 2000-01-10 Timothy M. Richardson Ultramicroscope a contraste inverse et methode
JP4825426B2 (ja) * 2005-01-31 2011-11-30 財団法人 東京都医学総合研究所 生物顕微鏡に用いる暗視野照明装置
WO2008130445A2 (fr) * 2006-11-10 2008-10-30 Advanced Analytical Technologies, Inc. Détection rapide de microorganismes dans des fluides
US20100212436A1 (en) * 2009-02-25 2010-08-26 Erik Axel Swenson Single use sterile slit impact sampling cassette with rotatable capture tray
US8849006B2 (en) * 2009-03-25 2014-09-30 Quorum Technologies Inc. Darkfield imaging system and methods for automated screening of cells
CA2774422C (fr) * 2009-10-12 2017-08-29 Ventana Medical Systems, Inc. Contraste multi-modalites et rendu de contexte en fond clair pour la determination amelioree d'une pathologie et une detection de multiples analytes dans un tissu
JP2011212013A (ja) * 2010-03-17 2011-10-27 Elmex Ltd フィルム型培地による微生物検査方法およびこの微生物検査方法に用いる画像取込ツ−ル
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JP5799653B2 (ja) * 2011-08-17 2015-10-28 富士通株式会社 画像処理装置、像を生成する方法並びにシステム
FR2986237A1 (fr) 2012-01-27 2013-08-02 Advencis Dispositif de detection rapide de micro-organismes
DK3004838T3 (da) * 2013-05-28 2022-06-20 Chemometec As Billeddannende cytometer
JP6229365B2 (ja) * 2013-08-06 2017-11-15 富士通株式会社 コロニーカウント装置、コロニーカウント方法、およびコロニーカウントプログラム
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FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie

Also Published As

Publication number Publication date
US20240185392A1 (en) 2024-06-06
CN113159262A (zh) 2021-07-23
GB202105015D0 (en) 2021-05-26
US20210318225A1 (en) 2021-10-14
JP2021168650A (ja) 2021-10-28
FR3109159A1 (fr) 2021-10-15
GB2595565A (en) 2021-12-01

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