CN102660443A - Healthcare wine capable of relieving physical fatigue and preparation method of healthcare wine - Google Patents
Healthcare wine capable of relieving physical fatigue and preparation method of healthcare wine Download PDFInfo
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Abstract
The invention belongs to the technical field of healthcare food, and particularly relates to healthcare wine capable of relieving physical fatigue and a preparation method of the healthcare wine. The healthcare wine comprises, by weight, from 18 to 40 parts of Chinese herbal medicine raspberry, from 22 to 32 parts of mulberry fruit, from 18 to 42 parts of wolfberry fruit, from 12 to 20 parts of longan pulp, from 10 to 26 parts of fructus alpiniae oxyphyllae and from 20 to 40 parts of rhizoma polygonati. The utilization quantity of white spirit is 8-13 times of the total weight of traditional Chinese medicinal materials. The traditional Chinese medicinal materials are edible traditional Chinese medicine, and are extracted by the maotai-flavor white spirit, conditioned, filtered and filled to form the healthcare wine. An animal experiment proves that the healthcare wine has a healthcare effect of relieving physical fatigue.
Description
Technical field
The present invention relates to a kind of health-care white spirit and preparation method thereof, belong to the health food technology field with effect of relieving physical fatigue.
Background technology
Tired: as to be a comprehensive physiological process that relates to many Physiology and biochemistry factors; It is a kind of normal physiological phenomenon that human body mentality or physical exertion must occur during to certain phase; It had both indicated the temporary transient decline of the original work capacity of body, possibly be again the tendency that body develops into sick and wounded state.
China Ministry of Health changes " antifatigue " function into " alleviating physical fatigue " function in " the protective foods check and evaluation technique standard " of putting into effect in May, 2003." antifatigue " has a great difference with " alleviating physical fatigue ", and " antifatigue " protective foods can not be resisted all types of fatigues, and " alleviating physical fatigue " is that this type protective foods is set forth the most accurately.
Physical fatigue makes corporality tired again; Be since when the people for a long time, during the physical exertion campaign of hard intensity; Produced a large amount of metabolites in the body, like lactic acid, carbonic acid gas, serum urea nitrogen etc., this type material gathers in vivo; Stimulate human tissue cell and neural system, will make the people produce sense of fatigue.
Alleviating fatigue product in the market roughly can be divided into following several types: the firstth, and supplementing energy usually reaches and keeps the body normal physiological function, the purpose of relieving fatigue through replenishing the nutrition that is consumed in the motion.The secondth, the VITAMINs and the trace element of additional needed by human.The 3rd is that removing, the discharge of acceleration bodies intracellular metabolite material reach the antifatigue purpose through the function of the function, the particularly recycle system that improves biological organs.
The present invention brings into play the theoretical advantages of medicines and health protection such as China's tradition dietotherapy, traditional Chinese medicine; The Chinese medicine that the effect of help property is arranged to the medical book record carries out the anti-fatigue effect evaluation, and the functional factor that certain effect is arranged is carried out deep research; Provide a kind of through improving the function of biological organs; The function of the recycle system particularly, removing, the discharge of acceleration bodies intracellular metabolite material reach the function of alleviating fatigue food that the alleviating fatigue purpose has national characters.
Summary of the invention
The purpose of this invention is to provide a kind of health-care white spirit and preparation method thereof with effect of relieving physical fatigue; Its Formula Design is to be guidance with the theory of traditional Chinese medical science; Through the liver soothing and the spleen invigorating, kidney tonifying, essence replenishing, replenishing qi and blood are combined together each other; By the capable biological organs that is transported to of alcohol, reach and alleviate the purpose of improving physical fatigue.
Said health-care white spirit with effect of relieving physical fatigue is prepared from the Chinese medicinal materials raw material Chinese medicinal materials raw material and liquor by weight ratio: raspberry 18-40 part, Fructus Mori 22-32 part; Wolfberry fruit 18-42 part; Longan pulp 12-20 part, Sharpleaf Galangal Fruit 10-26 part, sealwort 20-40 part; The liquor consumption is 8-13 a times of Chinese medicinal materials raw material gross weight;
When directly adopting Chinese liquor extraction Chinese medicinal materials method, liquor preferably ethanol degree is 45%～50% (alcoholic strength is a volume percent); After adopting the water extraction Chinese medicinal materials, when using the white spirit mixing method again, liquor preferably ethanol degree is 65%～70% (alcoholic strength is a volume percent);
Said liquor is preferably Maotai-flavor liquor;
Said part is weight unit such as g, kg, ton.
Said health-care white spirit dose with effect of relieving physical fatigue is recommended as every day 1-2 time, each 25-50ml.
Said preparation method with health-care white spirit of effect of relieving physical fatigue is:
Behind the Chinese medicinal materials picking of raw material, clean up, mix after cataclasm, adopt Chinese medicinal materials gross weight 5-8 45%～50% liquor (preferred Maotai-flavor liquor) doubly to adopt circulatory maceration to extract 3-5 days, filter, win all over extracting solution and filter residue; Then, in filter residue, add Chinese medicinal materials gross weight 3-5 45%～50% liquor (preferred Maotai-flavor liquor) doubly again and adopt circulatory maceration to extract 1-3 days, filter, second time extracting solution; Secondary raffinate is merged, after stirring, left standstill 24 hours, filtration promptly gets.Said liquor preferably ethanol degree is 45%～50%.
The preparation method of said health-care white spirit with effect of relieving physical fatigue also can for:
Behind the Chinese medicinal materials picking of raw material, clean up, mix after cataclasm, adopt 5-8 times of pure water of Chinese medicinal materials gross weight, 65 ℃-95 ℃ were extracted 3 hours, filtered, and won all over extracting solution and filter residue; Then, in filter residue, add 3-5 times of pure water of Chinese medicinal materials gross weight again, 65 ℃-95 ℃ were extracted 1 hour, filtered, and got second time extracting solution; Secondary filtrating merges, and adopts vacuum concentration, is prepared into the liquid concentrator that proportion is 1.2-1.4; Liquid concentrator and Chinese medicinal materials raw material gross weight 5-6 65%～70% liquor (preferred Maotai-flavor liquor) is doubly mixed with the pure water (by weight) of 0.4～0.55 times of liquor consumption, after stirring, left standstill 24 hours, filtration promptly gets.
The traditional Chinese medical science thinks, the generation of muscle power is the basis with the qi and blood, is tie with channels and collaterals, and the mutual coordination through function of five internal organs realizes.Wherein, kidney storing essence master bone is the prime mover of muscle power generation; Spleen is a source of generating QI and blood, and main limb muscle is participated in the body motion; Heart governing blood and vessels guarantees the energy supply of whole body through blood circulation; Lung governing qi, department breathe, court's hundred arteries and veins, promote blood and in blood vessels, move; Liver controlling conveyance and dispersion, main store blood, main muscle and be that " source of endurance " is the adjusting core of physical fatigue.Moving process is wasting QI-blood on the one hand, on the one hand spiritual mood high-pressure and make the body internal organs be in a kind of temporary transient nonequilibrium state.Insufficiency of vital energy and blood, the five internal organs regulatory function descend or imbalance all can cause physical fatigue.
Raspberry: it is sweet, sour to distinguish the flavor of, slightly warm in nature; Return liver, kidney channel.Has liver-kidney tonifying, kidney tonifying Yuanyang, the cloudy gas of kidney-nourishing and the effect of specially growing essence and blood.
Fructus Mori: it is sweet, sour to distinguish the flavor of, cold nature; Return liver, kidney, the heart channel of Hang-Shaoyin.Have and tonify the liver and kidney, nourishing YIN and benefiting blood, the effect of promoting the production of body fluid and moisturizing.
Wolfberry fruit: sweet, the property of distinguishing the flavor of is flat; Return liver, kidney channel.Nourishing the liver, nourshing kidney, moistening lung.Have nourishing liver and kidney, beneficial intensive culture blood, the screen that disappears that makes eye bright, the effect that moistens the lung and relieve the cough.
Longan pulp: warm in nature, the flavor sweet; Go into the heart, the spleen channel.Heart-spleen boosting fills blood; Heart tonifying and tranquilizing, the beneficial spleen that nourishes blood has good nourishing benefiting action.Be used for insufficiency of vital energy and blood, the body virtual force is weak when waiting disease, and the gas that these article can tonifying the spleen and stomach can be mended nutrient QI and blood being insufficient again.
Sharpleaf Galangal Fruit: flavor is hot, and temperature is nontoxic; Return spleen, kidney channel.The temperature spleen, warm kidney, solid and gas, puckery essence.It is supporing yang to warm up kidney, again can be warm spleen benefit gas, and the hot cold temperature of loosing is arranged, inducing astrigency is the merit of unit down, looses can play face and originally set upright, puckery final minification is urinated, the effect of giving consideration to both the incidental and fundamental.
Sealwort: it is sweet to distinguish the flavor of, and property is flat; Return spleen, lung, kidney channel.Boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney-nourishing.Have tonifying speen and tonifying kidney, nourshing kidney moistening lung, the profit cardiopulmonary, invigorate the spleen and benefit qi, boosting qi and nourishing yin, strengthening the bones and tendons effect.
Maotai-flavor liquor: the alcoholic strength temperature, flavor is hot and bitter sweet, and warm promoting blood circulation is arranged, the diffusing efficacy of a drug of a surname, warm stomach, the dispersing wind and cold of dispelling rouses oneself yang-energy, effects such as Ginseng Extract.Maotai-flavor liquor is unique can playing a role in health care in the wine, to the minimum liquor of actual bodily harm.Because its special making method, far different in the liquor of other odor types, comparatively speaking, volatile substances is few, so little to the stimulation of human body, healthy.Acidity is high, be 3 to 5 times of other wine, and be main mainly with acetate and lactic acid, according to theory of traditional Chinese medical science, sour main taste, protect the liver, the ability vessel softening; Doctor trained in Western medicine thinks that also food acid helps health.Also have materials such as SOD and rhMT in the sauce cordiale, wherein SOD is the single-minded scavenging agent of oxyradical, and its major function is to remove unnecessary radical in the body, and antitumor, antifatigue, antiviral, antidotal effect are obvious.
Longan, sealwort, Sharpleaf Galangal Fruit kidney tonifying and qi invigorating, invigorating spleen and nourishing heart in the side, raspberry, Fructus Mori, wolfberry fruit nourishing marrow and benefitting semen, nourishing the liver and kidney, all medicines share, and by relaxing through invigorating blood circulation, dispersing warm leading to of wine liquid, reach the merit of income qi and blood, invigorating the liver and kidney.Wine assisting drug, wine help the medicine gesture, through the qi and blood help, the five internal organs are regulated, and promote the functional recovery that body has descended, and then reach the health-care effect of alleviating physical fatigue.
The used Chinese medicinal materials raw material of the present invention raspberry, Fructus Mori, wolfberry fruit, longan pulp, Sharpleaf Galangal Fruit, sealwort be food be again the material that medicine or allow uses as healthy food material, the quality of raw material meets the requirement of Pharmacopoeia of People's Republic of China 2010 version (an one); The present invention is not to use traditional material with alleviating physical fatigue and medicinal material; But the choose reasonable raw material uses through the mutual compatibility of the unitized design of prescription; Through improving the function of biological organs; Reach, motor capacity low inferior alleviate and improvement effect aching and limp, be applicable to the physical fatigue disease that middle-aged and old a variety of causes cause malaise, four limbs due to overtired.Prove that through the security toxicological evaluation these article are nontoxic without any side effects; Through the function assessment test, these article meet " protective foods check and evaluation technique standard " " alleviating physical fatigue function " evaluation index, have the physical-fatigue-relieving health effect.
Embodiment
Embodiment 1
1, take by weighing following raw materials in weight:
Raspberry 18kg, Fructus Mori 22kg, wolfberry fruit 18kg, longan pulp 12kg, Sharpleaf Galangal Fruit 10kg, sealwort 20kg.
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 45% Maotai-flavor liquor of 5 times of weight to adopt circulatory maceration to extract 3 days, filter, win all over extracting solution and filter residue.
3,45% the Maotai-flavor liquor that in filter residue, adds 3 times of weight of Chinese medicinal materials gross weight again adopts circulatory maceration to extract 2 days, filter, second time extracting solution; Secondary raffinate is merged, after stirring, left standstill 24 hours, filtration promptly gets.
Embodiment 2
1, take by weighing following materials of weight proportions:
Raspberry 40kg, Fructus Mori 32kg, wolfberry fruit 42kg, longan pulp 20kg, Sharpleaf Galangal Fruit 26kg, sealwort 40kg.
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 45% Maotai-flavor liquor of 8 times of weight to adopt circulatory maceration to extract 5 days, filter, win all over extracting solution and filter residue.
3,45% the Maotai-flavor liquor that in filter residue, adds 5 times of weight of Chinese medicinal materials gross weight again adopts circulatory maceration to extract 3 days, filter, second time extracting solution; Secondary raffinate is merged, after stirring, left standstill 24 hours, filtration promptly gets.
Embodiment 3
1, take by weighing following raw materials in weight:
Raspberry 35kg, Fructus Mori 25kg, wolfberry fruit 30kg, longan pulp 15kg, Sharpleaf Galangal Fruit 15kg, sealwort 28kg.
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 45% Maotai-flavor liquor of 6 times of weight to adopt circulatory maceration to extract 4 days, filter, win all over extracting solution and filter residue.
3,45% the Maotai-flavor liquor that in filter residue, adds 4 times of weight of Chinese medicinal materials gross weight again adopts circulatory maceration to extract 2 days, filter, second time extracting solution; Secondary raffinate is merged, after stirring, left standstill 24 hours, filtration promptly gets.
Instance 4
1, take by weighing following materials of weight proportions:
Raspberry 18kg, Fructus Mori 22kg, wolfberry fruit 18kg, longan pulp 12kg, Sharpleaf Galangal Fruit 10kg, sealwort 20kg.
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 5 times of weight pure water, 65 ℃-95 ℃ were extracted 3 hours, filtered, and won all over extracting solution and filter residue.
3, in filter residue, add Chinese medicinal materials gross weight 3 times of weight pure water again, 65 ℃-95 ℃ were extracted 1 hour, filtered, and got second time extracting solution.
4, secondary filtrating merges, and adopts vacuum concentration, is prepared into the liquid concentrator that proportion is 1.2-1.4, liquid concentrator weight 100kg.
5, the 100kg liquid concentrator is mixed with the Maotai-flavor liquor of 510.54kg 70% and the pure water of 271kg, after stirring, left standstill 24 hours, filtration promptly gets.
Embodiment 5
1, take by weighing following materials of weight proportions:
Raspberry 40kg, Fructus Mori 32kg, wolfberry fruit 42kg, longan pulp 20kg, Sharpleaf Galangal Fruit 26kg, sealwort 40kg.
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 8 times of weight pure water, 65 ℃-95 ℃ were extracted 3 hours, filtered, and won all over extracting solution and filter residue.
3, in filter residue, add 5 times of weight pure water of Chinese medicinal materials gross weight again, 65 ℃-95 ℃ were extracted 1 hour, filtered, and got second time extracting solution.
4, secondary filtrating merges, and adopts vacuum concentration, is prepared into the liquid concentrator that proportion is 1.2-1.4, liquid concentrator weight 200kg.
5, the 200kg liquid concentrator is mixed with the Maotai-flavor liquor of 1021.08kg 70% and the pure water of 542kg, after stirring, left standstill 24 hours, filtration promptly gets.
Embodiment 6
1, take by weighing following materials of weight proportions:
Raspberry 35kg, Fructus Mori 25kg, wolfberry fruit 30kg, longan pulp 15kg, Sharpleaf Galangal Fruit 15kg, sealwort 28kg
2, with behind the picking of raw material, clean up, mix after cataclasm, adopt 6 times of weight pure water, 65 ℃-95 ℃ were extracted 3 hours, filtered, and won all over extracting solution.
3, adopt 4 times of weight pure water again, 65 ℃-95 ℃ were extracted 1 hour, filtered, and got second time extracting solution.
4, secondary filtrating merges, and adopts vacuum concentration, is prepared into the liquid concentrator that proportion is 1.2-1.4, liquid concentrator weight 150kg.
5, the 150kg liquid concentrator is mixed with the Maotai-flavor liquor of 765.81kg 70% and the pure water of 406.5kg, after stirring, left standstill 24 hours, filtration promptly gets.
Product alleviating physical fatigue function test of the present invention
1, method
1.1 laboratory animal divides into groups and medication
After mouse flexibility fed for 1 week; Be divided into 4 batches (48 every batch) at random; The every batch of animal is divided into control group, the invention basic, normal, high dose groups of product (12 every group) at random, and the basic, normal, high dosage of invention product (8.3,16.7,33.2g/ (kgbwd)) (be equivalent to respectively human body recommended dose 5,10 and 20 times) group is irritated stomach respectively and invented wine liquid, irritates the long-pending 0.2mL/10g of body of stomach; Control group is irritated stomach equal-volume zero(ppm) water; 30d according to Ministry of Health of the People's Republic of China's " protective foods check and evaluation technique standard " (2003) method, carries out swimming with a load attached to the body experiment, serum urea nitrogen, liver starch, blood lactic acid content determination experiment respectively continuously.
Annotate: this test uses the invention product to be instance 4 products.
1.2 index detects
Measure the animal swimming with a load attached to the body time; Serum urea nitrogen (BUN) assay adopts sharp Bath AU400 automatic clinical chemistry analyzer difficult to understand; Liver starch, blood lactic acid content are measured by the test kit specification sheets.
2, testing data
Table 1 pair mice serum urea nitrogen content, hepatic glycogen content and the influence of swimming with a load attached to the body time (x ± s, n=12)
| Group | Serum urea nitrogen/(mmol/L) | Hepatic glycogen content/(mg/g hepatic tissue) | Swimming with a load attached to the body time/min |
| Control group | 8.76±1.36 | 23.3±4.0 | 2.98±0.55 |
| Low dose group | 8.28±1.37 | 25.7±4.8 | 4.31±0.91(1) |
| Middle dose groups | 7.46±1.27(1) | 30.0±5.8(1) | 4.41±1.14(1) |
| High dose group | 8.02±0.95 | 30.7±5.2(1) | 4.86±1.35(1) |
Annotate: (1) expression and control group comparing difference have significance (P < 0.05).
Table 1 shows that behind the dose groups mouse swimming with a load attached to the body 90min, serum urea nitrogen is lower than control group, and difference has significance in the product of the present invention; Middle and high dose groups Mouse Liver glycogen levels all is higher than control group, and difference has significance, shows that product of the present invention can reduce motion back serum urea nitrogen level, and can increase the liver starch deposit of mouse.Each dose groups mouse swimming with a load attached to the body time all prolongs than control group, and difference has significance.
The influence of table 2 pair mouse blood lactic acid content (x ± s, n=12)
Annotate: (1) each dose groups and control group relatively have significant difference, P＜0.01
Table 2 shows; Irritate each dose groups mouse swimming back 0 of stomach product of the present invention, 20min blood lactic acid content and control group reduction is more all arranged; Wherein swimming back 20min high dose group blood lactic acid content and control group relatively have statistical significance (P＜0.01); TG-AUC and control group are apparent in view to be reduced, and difference has statistical significance (P＜0.01).
Conclusion (of pressure testing): this animal experiment study is the result show, product of the present invention can increase the animal's liver glycogen contents deposit, prolongs the animal swimming with a load attached to the body time, reduces serum urea nitrogen and blood lactic acid content, explains that product of the present invention has effect of relieving physical fatigue.
Claims (4)
1. the health-care white spirit with effect of relieving physical fatigue is characterized in that, is prepared from by weight ratio Chinese medicinal materials raw material and liquor; Chinese medicinal materials raw material: raspberry 18-40 part; Fructus Mori 22-32 part, wolfberry fruit 18-42 part, longan pulp 12-20 part; Sharpleaf Galangal Fruit 10-26 part, sealwort 20-40 part; The liquor consumption is 8-13 a times of Chinese medicinal materials raw material gross weight.
2. one kind prepares its preparation method of health-care white spirit according to claim 1; It is characterized in that: behind the Chinese medicinal materials picking of raw material; Clean up, mix after cataclasm, adopt Chinese medicinal materials gross weight 5-8 45%～50% liquor doubly to adopt circulatory maceration to extract 3-5 days; Filter, win all over extracting solution and filter residue; Then, in filter residue, add Chinese medicinal materials gross weight 3-5 45%～50% liquor doubly again and adopt circulatory maceration to extract 1-3 days, filter, second time extracting solution; Secondary raffinate is merged, after stirring, left standstill 24 hours, filtration promptly gets.
3. one kind prepares its preparation method of health-care white spirit according to claim 1, it is characterized in that: behind the Chinese medicinal materials picking of raw material, clean up; Mix after cataclasm, adopt 5-8 times of pure water of Chinese medicinal materials gross weight, 65 ℃-95 ℃ were extracted 3 hours; Filter, win all over extracting solution and filter residue; Then, in filter residue, add 3-5 times of pure water of Chinese medicinal materials gross weight again, 65 ℃-95 ℃ were extracted 1 hour, filtered, and got second time extracting solution; Secondary filtrating merges, and adopts vacuum concentration, is prepared into the liquid concentrator that proportion is 1.2-1.4; Liquid concentrator and Chinese medicinal materials raw material gross weight 5-6 65%～70% liquor and the pure water of 0.4～0.55 times of liquor consumption doubly mixed, after stirring, left standstill 24 hours, filtration promptly gets.
4. according to the arbitrary described health-care white spirit of claim 1-3, it is characterized in that: said liquor is Maotai-flavor liquor.
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| CN103103097A (en) * | 2013-01-16 | 2013-05-15 | 丽水市林业科学研究院 | Rhizoma polygonati kidney tonifying wine and preparation method thereof |
| CN103251803A (en) * | 2013-05-24 | 2013-08-21 | 安徽援康现代中药资源有限公司 | Formula and process of health-assistant wine |
| CN103351989A (en) * | 2013-07-30 | 2013-10-16 | 孟宗科 | Preparation method of wolfberry health wine |
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| CN103103097B (en) * | 2013-01-16 | 2015-05-27 | 丽水市林业科学研究院 | Rhizoma polygonati kidney tonifying wine and preparation method thereof |
| CN103103097A (en) * | 2013-01-16 | 2013-05-15 | 丽水市林业科学研究院 | Rhizoma polygonati kidney tonifying wine and preparation method thereof |
| CN103251803A (en) * | 2013-05-24 | 2013-08-21 | 安徽援康现代中药资源有限公司 | Formula and process of health-assistant wine |
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| CN103351989A (en) * | 2013-07-30 | 2013-10-16 | 孟宗科 | Preparation method of wolfberry health wine |
| CN103393841A (en) * | 2013-07-30 | 2013-11-20 | 孟宗科 | Preparation method of hovenia dulcis thunb health wine |
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| CN104560562A (en) * | 2014-12-25 | 2015-04-29 | 陆莎 | Mulberry health caring wine |
| CN106072513A (en) * | 2016-06-03 | 2016-11-09 | 赵斌 | A kind of have the food of nourishing blood function, health product or pharmaceutical composition and preparation method thereof |
| CN106010898A (en) * | 2016-07-25 | 2016-10-12 | 河南美邦生物科技有限公司 | Pure plant healthcare wine and preparation technology thereof |
| CN106753955A (en) * | 2016-12-15 | 2017-05-31 | 珠海聚焦先导生物研究院有限公司 | A kind of Female health wine for improving muscle power |
| CN110144276A (en) * | 2019-05-15 | 2019-08-20 | 四川大学 | A kind of preparation process with anti-fatigue effect health liquor |
| CN110408507A (en) * | 2019-08-26 | 2019-11-05 | 劲牌有限公司 | A kind of health liquor and preparation method thereof with anti-oxidation function |
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| CN113621466A (en) * | 2021-08-05 | 2021-11-09 | 劲牌有限公司 | Nostoc sphaeroids kutz health-preserving soy sauce wine and preparation process thereof |
| CN115191534A (en) * | 2022-06-30 | 2022-10-18 | 桂林名士威食品有限公司 | Fatigue-relieving bull strong beverage and preparation method thereof |
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| CN102660443B (en) | 2013-06-05 |
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Address after: 102629 Daxing District biological pharmaceutical industry base, Yongxing Road, 28, Beijing Patentee after: Beijing Sanqi Herbal Technology Co., Ltd. Address before: 102629 Daxing District biological pharmaceutical industry base, Yongxing Road, 28, Beijing Patentee before: Beijing Sanqibencao Medical Technology Co., Ltd. |
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