CN102599096A - Method for carrying out incubation on neolissochilus benasi through artificial fertilization - Google Patents
Method for carrying out incubation on neolissochilus benasi through artificial fertilization Download PDFInfo
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- CN102599096A CN102599096A CN2012101124178A CN201210112417A CN102599096A CN 102599096 A CN102599096 A CN 102599096A CN 2012101124178 A CN2012101124178 A CN 2012101124178A CN 201210112417 A CN201210112417 A CN 201210112417A CN 102599096 A CN102599096 A CN 102599096A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for carrying out incubation on neolissochilus benasi through artificial fertilization, which belongs to the technical field of fish product breeding. The method comprises the following steps of: injecting DOM (domperidone maleate) and LRH-A2 (lutein releasing hormone A2) into gonadosomatic neolissochilus benasi (1mg of DOM and 1 mu g of LRH per kilogram of neolissochilus benasi are injected in per kilogram of gonadosomatic neolissochilus benasi), wherein the injection volume is halved for male neolissochilus benasi, and the effect time is 15 hours at a water temperature of 22 DEG C; extruding ovums and sperms of the gonadosomatic neolissochilus benasi into a container, stirring the ovums and the sperms for 30 seconds by using chicken feather, then adding clear water at a temperature of 22 DEG C into the container (water preferably just submerges the ovums and the sperms), after stirring the obtained mixture for 20 seconds by using the chicken feather, carrying out washing three times by using clear water, then taking the obtained fertilized ovums for backup incubation; putting the fertilized ovums into an aqueous captan solution (0.15 ppm) to soak for 15 minutes, then putting the fertilized ovums into a water-filled dissecting tray and a shallow-water basin (in which the water level is preferably 3-5 cm higher than the ovums) for incubation, wherein 3000-5000 ovums with an ovum diameter of 2.2-2.8 mm are put in each basin, the position of a water-filled container is 50-80 cm higher than the dissecting tray and the shallow-water basin; sucking water by using a small-sized oxygen hose and conveying the water to the dissecting tray and the shallow-water basin to carry out incubation for 120 hours so as to get fish fries, wherein the temperature of the water is 22-23 DEG C, and the pH value of the water is 7.8-8.5. The method disclosed by the invention has the advantages of low cost, easiness in operation, and high incubation rate.
Description
Technical field:
The present invention relates to the artificial insemination hatching m of the new light lip of a kind of soft fin fish, belong to fishery-ies product cultural technique field.
Background technology:
Sunbeam lips soft fin fish (Neolissochilus? Benasi) (Pellegrin? &? Chevey, 1936), formerly known as soft-finned four required Barb (Barbodes? Benasi), local fish, flowers, belonging carp (Cyprinidae) Barbinae (Barbinae) Sunbeam lip genus (Neolissochilus).In history, the new light lip of soft fin fish is Yuanjiang River basin fisherman's a main capture object, mainly is distributed in the ground such as river mouth, Jiang Cheng, Xichou, Yuanjiang River in Yunnan, is distributed in Vietnam overseas.According to the survey showed that in recent years; Owing to catching intensity is big, the step hydropower station is developed and reasons such as weakness are realized in protection; It is very serious that the new light lip of soft fin fingerling crowd is failed, and its population is very accidental in basin, Li Xian river, the present only bigger population of Wenshan Prefecture Xichou County preservation in Yunnan.The present situation of the new light lip of soft fin fish has caused the very big concern of relevant department and hydroelectric development relevant enterprise, and has taked a series of protection action.For the rare Yunnan original inhabitants' stock of fish of appearance in protection, development and utilization; International Li Xian river basin hydroelectric development Co., Ltd of Yunnan Datang and State Grid Yunnan Ah Mojiang electricity generating corporation, Ltd, trust Kunming Institute of Zoology, Chinese Academy of Sciences have carried out the artificial fecundation of the new light lip of soft fin fish the research of releasing.
Through the document retrieval, do not see and identical report of the present invention.
Summary of the invention:
The present invention provides the artificial insemination hatching m of the new light lip of a kind of soft fin fish; Purpose is to recover Li Xianjiang and the A Mojiang fish because of influenced by the step hydropower station construction---this rare and endangered resource of the new light lip of soft fin fish; And make it become a new pond culture kind, realize its intensification, industrialization.
The present invention is through the new light lip of soft fin fish injection maleic acid DOM and luteinizing hormone releasing hormone A to maturation
2, manual work is lured into female (hero) steck ovum (essence) takes the artificial insemination method to hatch fry, and carries out fry rearing research.Result of study is to lay the foundation with enforcement heredity management the history of life of the new light lip of soft fin fish, and accumulates experience for setting up the new light lip of a practicable soft fin of cover fish artificial insemination hatching m from now on.
The artificial insemination hatching m of the new light lip of soft fin of the present invention fish, its concrete steps are following:
A. dry method fertilization: the new light lip of the soft fin fish to gonad maturity is injected by luteotropin releasing hormone d-ala analog (LHRH-A
2The mixing ocyodinic that)+maleic acid DOM (DOM) is formed, dosage is by per kilogram of body weight 1mg maleic acid DOM+1 μ g luteinizing hormone releasing hormone A
2, the milter injection volume reduces by half, and 15 hours its effect time was carried out artificial dry method fertilization when 22 ℃ of water temperatures;
Artificial dry method fertilization: ovum and the sperm with the new light lip of sexually matured soft fin fish is squeezed in the preprepared container from its belly gently respectively, stirs gently 30 seconds with chicken feather at once, mixes 22 ℃ of clear water again, is advisable to flood smart ovum; Then again with chicken feather stir gently after 20 seconds clean 3 times with clear water after, prepare to hatch;
B. artificial incubation: the special aqueous solution immersion of gram bacterium that elder generation puts into 0.15ppm with fertilized egg carried out disinfection in 15 minutes; Be filled with water dissecting pan and shallow laver of fertilized egg putting into after the sterilization hatched, and water level is advisable to exceed fish-egg 3～5cm, and every basin is put 3000～5000 on the ovum that ovum directly is 2.2～2.8mm; The position of water container is higher than dissecting pan and shallow laver 50～80cm; Get into dissecting pan and shallow laver through small size oxygen hose sucking-off water, carry out the flowing water hatching, water temperature 22-23 ℃; PH7.8-8.5 is hatched and was obtained fry in 120 hours.
After treating the fry opening, throw something and feed every day 3 times, the bait of throwing something and feeding is respectively: aioli, soya-bean milk, concrete feeding volume are that per thousand tail prelarvas are pressed yolk half, dried soya bean 30g grind the pulping full pool spilling head.7-15 behind the fry opening days, the wheel animalcule 3 times of throwing something and feeding every day, the wheel animalcule feeding volume is ten thousand/m of 200-300
3After 45 days behind the fry opening; Begin the 111 broken carp fingerling fish meal (particle diameter≤0.5mm that throw something and feed; Kunming branch company of Tongwei Co., Ltd.); Every day, feeding volume was pressed the calculating feeding volume of 2%-5% feed coefficient, and took the circumstances into consideration plus-minus according to the feed situation of water temperature and fish, raised by conventional raising method.
That the present invention has is easy and simple to handle, cost is low, incubation rate is up to 92% advantage.
Embodiment:
On September 6th, 2011 during 18:00 about, since the new light lip of the soft fin fish of raising and train in 2005 of picking up from the mountain of papers Xichou, choose 12 groups of sexual maturity parent populations, female in 1: male 3 ratio is injected; According to following method; Carried out artificial insemination September 7 during 9:00, obtain 45360 in fertilized egg, September 12 accomplished hatching with following method; Hatch fry 42000 tails, incubation rate is up to 92%.Specific practice is following:
A. dry method fertilization: the new light lip of the soft fin fish to gonad maturity is injected by luteotropin releasing hormone d-ala analog (LHRH-A
2The mixing ocyodinic that)+maleic acid DOM (DOM) is formed, dosage is by per kilogram of body weight 1mg maleic acid DOM+1 μ g luteinizing hormone releasing hormone A
2, the milter injection volume reduces by half, and 15 hours its effect time was carried out artificial dry method fertilization when 22 ℃ of water temperatures;
Artificial dry method fertilization: ovum and the sperm with the new light lip of sexually matured soft fin fish is squeezed in the preprepared container from its belly gently respectively, stirs gently 30 seconds with chicken feather at once, mixes 22 ℃ of clear water again, is advisable to flood smart ovum; Then again with chicken feather stir gently after 20 seconds clean 3 times with clear water after, prepare to hatch;
B. artificial incubation: the special aqueous solution immersion of gram bacterium that elder generation puts into 0.15ppm with fertilized egg carried out disinfection in 15 minutes; Be filled with water dissecting pan and shallow laver of fertilized egg putting into after the sterilization hatched, and water level is advisable to exceed fish-egg 3cm or 4cm or 5cm, and every basin is put ovum and directly is 3000 or 4000 or 5000 on the ovum of 2.2mm or 2.mm or 2.8mm; The position of water container is higher than dissecting pan and shallow laver 50cm or 65cm or 80cm; Get into dissecting pan and shallow laver through small size oxygen hose sucking-off water, carry out the flowing water hatching, 22 ℃ or 23 ℃ of water temperatures; PH7.8 or pH 8.0 or pH 8.5 are hatched and were obtained fry in 120 hours.
After treating the fry opening, throw something and feed every day 3 times, the bait of throwing something and feeding is respectively: aioli, soya-bean milk, concrete feeding volume are that per thousand tail prelarvas are pressed yolk half, dried soya bean 30g grind the pulping full pool spilling head.Behind the fry opening 7 to 15 days, the wheel animalcule 3 times of throwing something and feeding every day, the wheel animalcule feeding volume is 2,000,000/m
3Or 2,500,000/m
3Or 3,000,000/m
3After 45 days behind the fry opening; Begin the 111 broken carp fingerling fish meal (particle diameter≤0.5mm that throw something and feed; Kunming branch company of Tongwei Co., Ltd. produces); Every day, feeding volume was pressed the calculating feeding volume of 2% or 3% or 5% feed coefficient, and took the circumstances into consideration plus-minus according to the feed situation of water temperature and fish, raised by conventional raising method.
Claims (1)
1. the artificial insemination hatching m of the new light lip of a soft fin fish is characterized in that the concrete steps of this artificial insemination hatching m are following:
A. dry method fertilization: the new light lip of the soft fin fish to gonad maturity is injected by luteotropin releasing hormone d-ala analog (LHRH-A
2The mixing ocyodinic that)+maleic acid DOM (DOM) is formed, dosage is by per kilogram of body weight 1mg maleic acid DOM+1 μ g luteinizing hormone releasing hormone A
2, the milter injection volume reduces by half, and 15 hours its effect time was carried out artificial dry method fertilization when 22 ℃ of water temperatures;
Artificial dry method fertilization: ovum and the sperm with the new light lip of sexually matured soft fin fish is squeezed in the preprepared container from its belly gently respectively, stirs gently 30 seconds with chicken feather at once, mixes 22 ℃ of clear water again, is advisable to flood smart ovum; Then again with chicken feather stir gently after 20 seconds clean 3 times with clear water after, prepare to hatch;
B. artificial incubation: the special aqueous solution immersion of gram bacterium that elder generation puts into 0.15ppm with fertilized egg carried out disinfection in 15 minutes; Be filled with water dissecting pan and shallow laver of fertilized egg putting into after the sterilization hatched, and water level is advisable to exceed fish-egg 3～5cm, and every basin is put 3000～5000 on the ovum that ovum directly is 2.2～2.8mm; The position of water container is higher than dissecting pan and shallow laver 50～80cm; Get into dissecting pan and shallow laver through small size oxygen hose sucking-off water, carry out the flowing water hatching, water temperature 22-23 ℃; PH7.8-8.5 is hatched and was obtained fry in 120 hours.
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| CN2012101124178A CN102599096A (en) | 2012-04-17 | 2012-04-17 | Method for carrying out incubation on neolissochilus benasi through artificial fertilization |
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| CN2012101124178A CN102599096A (en) | 2012-04-17 | 2012-04-17 | Method for carrying out incubation on neolissochilus benasi through artificial fertilization |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
| CN103444610A (en) * | 2013-09-23 | 2013-12-18 | 云南省水产技术推广站 | Artificial propagation method for acrossocheilus yunnanensis |
| CN103461248A (en) * | 2013-09-23 | 2013-12-25 | 云南省水产技术推广站 | Fish fry cultivation method of acrossocheilus yunnanensis |
| CN103548740A (en) * | 2013-11-07 | 2014-02-05 | 武汉百瑞生物技术有限公司 | Method for producing gynoecial loaches |
| CN104509469A (en) * | 2014-12-24 | 2015-04-15 | 休宁县龙堂生态渔业科技有限公司 | High-density green ecological aquaculture method for acrossocheilus fasciatus |
| CN104805052A (en) * | 2015-04-27 | 2015-07-29 | 中国科学院昆明动物研究所 | Method for building neolissochilus banasi muscle cell line |
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| CN100425128C (en) * | 2006-09-08 | 2008-10-15 | 浙江省淡水水产研究所 | Method for artificial propagation of Elopichthys bambus |
| CN102077787A (en) * | 2010-11-17 | 2011-06-01 | 贵州省毕节地区水产技术推广站 | Artificial propagation technology for schizothorax grahami |
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2012
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| CN100425128C (en) * | 2006-09-08 | 2008-10-15 | 浙江省淡水水产研究所 | Method for artificial propagation of Elopichthys bambus |
| CN102077787A (en) * | 2010-11-17 | 2011-06-01 | 贵州省毕节地区水产技术推广站 | Artificial propagation technology for schizothorax grahami |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
| CN103098737B (en) * | 2013-02-26 | 2015-03-11 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
| CN103444610A (en) * | 2013-09-23 | 2013-12-18 | 云南省水产技术推广站 | Artificial propagation method for acrossocheilus yunnanensis |
| CN103461248A (en) * | 2013-09-23 | 2013-12-25 | 云南省水产技术推广站 | Fish fry cultivation method of acrossocheilus yunnanensis |
| CN103444610B (en) * | 2013-09-23 | 2014-09-17 | 云南省水产技术推广站 | Artificial propagation method for acrossocheilus yunnanensis |
| CN103548740A (en) * | 2013-11-07 | 2014-02-05 | 武汉百瑞生物技术有限公司 | Method for producing gynoecial loaches |
| CN104509469A (en) * | 2014-12-24 | 2015-04-15 | 休宁县龙堂生态渔业科技有限公司 | High-density green ecological aquaculture method for acrossocheilus fasciatus |
| CN104805052A (en) * | 2015-04-27 | 2015-07-29 | 中国科学院昆明动物研究所 | Method for building neolissochilus banasi muscle cell line |
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Application publication date: 20120725 |