CN102584989B - 用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒 - Google Patents

用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒 Download PDF

Info

Publication number
CN102584989B
CN102584989B CN201210020646.7A CN201210020646A CN102584989B CN 102584989 B CN102584989 B CN 102584989B CN 201210020646 A CN201210020646 A CN 201210020646A CN 102584989 B CN102584989 B CN 102584989B
Authority
CN
China
Prior art keywords
igg
polypeptide
sample
endos
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201210020646.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN102584989A (zh
Inventor
玛利亚·奥霍恩
安德斯·奥琳
福克·尼莫雅恩
马蒂亚斯·科林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genovis AB
Original Assignee
Genovis AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genovis AB filed Critical Genovis AB
Publication of CN102584989A publication Critical patent/CN102584989A/zh
Application granted granted Critical
Publication of CN102584989B publication Critical patent/CN102584989B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/641—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201210020646.7A 2007-09-14 2008-09-11 用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒 Expired - Fee Related CN102584989B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96008707P 2007-09-14 2007-09-14
US60/960,087 2007-09-14

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2008801155765A Division CN101889082A (zh) 2007-09-14 2008-09-11 用于解离Fcgamma-受体-IgG复合物以及纯化与检测IgG的方法和试剂盒

Publications (2)

Publication Number Publication Date
CN102584989A CN102584989A (zh) 2012-07-18
CN102584989B true CN102584989B (zh) 2015-05-13

Family

ID=40076765

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201210020646.7A Expired - Fee Related CN102584989B (zh) 2007-09-14 2008-09-11 用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒
CN2008801155765A Pending CN101889082A (zh) 2007-09-14 2008-09-11 用于解离Fcgamma-受体-IgG复合物以及纯化与检测IgG的方法和试剂盒

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN2008801155765A Pending CN101889082A (zh) 2007-09-14 2008-09-11 用于解离Fcgamma-受体-IgG复合物以及纯化与检测IgG的方法和试剂盒

Country Status (7)

Country Link
US (1) US8323908B2 (enExample)
EP (1) EP2190984B1 (enExample)
JP (1) JP5722627B2 (enExample)
CN (2) CN102584989B (enExample)
DK (1) DK2190984T3 (enExample)
PL (1) PL2190984T3 (enExample)
WO (1) WO2009033670A2 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002216863B8 (en) * 2000-12-21 2008-03-20 Id Biomedical Corporation Streptococcus pyogenes antigens and corresponding DNA fragments
US20120196310A1 (en) 2009-10-02 2012-08-02 Christiane Jaeger A-fucosylation detection in antibodies
WO2011059385A1 (en) * 2009-11-13 2011-05-19 Bengt Guss Immunizing composition for reducing streptococcal infections
WO2011149419A1 (en) 2010-05-26 2011-12-01 Intervacc Ab Vaccine against streptococcal infections based on recombinant proteins
GB201103780D0 (en) * 2011-03-04 2011-04-20 Hansa Medical Ab Treatment for ige-mediated disease
GB201115841D0 (en) * 2011-09-13 2011-10-26 Genovis Ab Protein and method
GB201201314D0 (en) * 2012-01-26 2012-03-07 Isis Innovation Composition
IN2014DN06806A (enExample) * 2012-02-10 2015-05-22 Univ Maryland
TW201404784A (zh) * 2012-07-27 2014-02-01 Univ Nat Chiao Tung 純化蛋白質的方法
PL2991683T3 (pl) 2013-05-02 2020-03-31 Glykos Finland Oy Koniugaty glikoproteiny lub glikanu z toksycznym ładunkiem
EP3160513B1 (en) 2014-06-30 2020-02-12 Glykos Finland Oy Saccharide derivative of a toxic payload and antibody conjugates thereof
GB201413240D0 (en) * 2014-07-25 2014-09-10 Hansa Medical Ab Method
GB201416849D0 (en) * 2014-09-24 2014-11-05 Genovis Ab Method
GB201502306D0 (en) 2015-02-12 2015-04-01 Hansa Medical Ab Protein
GB201502305D0 (en) 2015-02-12 2015-04-01 Hansa Medical Ab Protein
CN108026518B (zh) * 2015-07-16 2022-09-06 第一三共株式会社 新型endos突变型酶
CN105368808B (zh) * 2015-11-30 2019-04-30 苏州康聚生物科技有限公司 一种IdeS蛋白酶、其制备方法及应用
CN105368904A (zh) * 2015-11-30 2016-03-02 苏州康聚生物科技有限公司 一种免疫球蛋白g片段的制备方法及应用
AU2017214441B2 (en) 2016-02-04 2021-08-19 Genovis Ab New Streptococcal proteases
AU2017360807B2 (en) * 2016-11-18 2024-11-28 Biocon Limited Rapid and efficient de-glycosylation of glycoproteins
CN110031632A (zh) * 2019-04-03 2019-07-19 中国医学科学院北京协和医院 一种反映IgG4相关性疾病的疾病活动性的生物标志物及其用途
GB202002072D0 (en) * 2020-02-14 2020-04-01 Hansa Biopharma AB immunoglobulin detection and associated therapies
GB202007431D0 (en) 2020-05-19 2020-07-01 Hansa Biopharma AB Cysteine protease
CN111707835B (zh) * 2020-07-01 2022-11-11 珠海丽珠试剂股份有限公司 用于检测IgG4的组合物、试剂盒、应用及检测方法
CA3195177A1 (en) 2020-10-07 2022-04-14 Asklepios Biopharmaceutical, Inc. Therapeutic adeno-associated virus delivery of fukutin related protein (fkrp) for treating dystroglycanopathy disorders including limb girdle 2i (lgmd2i)
WO2022221529A1 (en) 2021-04-16 2022-10-20 Asklepios Biopharmaceutical, Inc. Rational polyploid aav virions that cross the blood brain barrier and elicit reduced humoral response
EP4079848A1 (en) 2021-04-22 2022-10-26 Genovis Ab Immunoglobulin cleaving enzyme
WO2024057095A1 (en) 2022-09-14 2024-03-21 Genovis Ab Mutant protease
CN115873833B (zh) * 2022-11-08 2024-08-16 上海泰昶生物技术有限公司 生产免疫球蛋白g降解酶的工程菌株和工艺
WO2025256977A1 (en) 2024-06-13 2025-12-18 Bayer Aktiengesellschaft Novel igm and igg cleaving enzymes
WO2025256978A1 (en) 2024-06-13 2025-12-18 Bayer Aktiengesellschaft Variants of igm and igg cleaving enzymes
GB202413764D0 (en) 2024-09-18 2024-10-30 Genovis Ab Immunoglobulin proteases and their uses

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019740A1 (en) * 1990-06-21 1991-12-26 Hightech Receptor Ab IgG-BINDING PROTEIN
WO2003051914A2 (en) * 2001-12-18 2003-06-26 Hansa Medical Research Aktiebolag Ides, an igg-degrading enzyme of streptococcus pyogenes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019740A1 (en) * 1990-06-21 1991-12-26 Hightech Receptor Ab IgG-BINDING PROTEIN
WO2003051914A2 (en) * 2001-12-18 2003-06-26 Hansa Medical Research Aktiebolag Ides, an igg-degrading enzyme of streptococcus pyogenes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A Novel Secreted Endoglycosidase from Enterococcus faecalis with Activity on Human Immunoglobulin G and Ribonuclease B;Mattias Collin, et al.;《Journal of Biological Chemistry》;20040521;第279卷(第21期);第22566页左栏第1-2段 *
Maria Allhorn,et al..Human IgG/FccR Interactions Are Modulated by Streptococcal IgG Glycan Hydrolysis.《PLOS ONE》.2008,第3卷(第1期),第1-12页. *
The impact of glycosylation on the biological function and structure of human immunoglobulins;ARNOLD JAMES N.,et al.;《ANNUAL REVIEW OF IMMUNOLOGY》;20070101;第25卷;第21-50页 *
The streptococcal protease IdeS modulates bacterial IgGFc binding and generates 1/2Fc fragments with the ability to prime polymorphonuclear leucocytes;Jenny Johansson S¨oderberg, et al.;《Molecular Immunology》;20080701;第45卷(第12期);第3348页2.4部分，第3347页右栏第2-4行 *

Also Published As

Publication number Publication date
DK2190984T3 (da) 2013-12-02
US8323908B2 (en) 2012-12-04
US20100317083A1 (en) 2010-12-16
WO2009033670A2 (en) 2009-03-19
EP2190984B1 (en) 2013-09-04
WO2009033670A8 (en) 2009-07-09
JP2010538623A (ja) 2010-12-16
WO2009033670A3 (en) 2009-04-30
CN102584989A (zh) 2012-07-18
CN101889082A (zh) 2010-11-17
EP2190984A2 (en) 2010-06-02
PL2190984T3 (pl) 2014-02-28
JP5722627B2 (ja) 2015-05-27

Similar Documents

Publication Publication Date Title
CN102584989B (zh) 用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒
CA2848230C (en) Endoglycosidase from streptococcus pyogenes and methods using it
Sjögren et al. On enzymatic remodeling of IgG glycosylation; unique tools with broad applications
EP3091032A1 (en) Humanized antibody or fragment thereof specific for cd3
WO2018097951A1 (en) Anti-mage-a3/a6 antibodies
CN120535635B (zh) 基于流式细胞术检测白细胞共同抗原的抗cd45抗体及其应用
Wang et al. The interplay of protein engineering and glycoengineering to fine‐tune antibody glycosylation and its impact on effector functions
JP5570990B2 (ja) 改変型ビオチン結合タンパク質
PE et al. O-linked glycosylation in focus
CN112375146B (zh) 检测Anti-CD19 CAR表达水平的抗独特性抗体及其应用
JP2017522289A (ja) 改善されたα−Ｖβ−８抗体
CN115611986B (zh) 针对人活化蛋白c的单克隆抗体及其制备和应用
CN107208128A (zh) 用于分析抗体样本中的聚集体的方法
KR20230116793A (ko) 세포의 농화를 위한 항체
US11306302B2 (en) Soluble Sortase A
US20180217151A1 (en) Reversible labeling of antigens in biological specimens
Malaker et al. A mucin-specific protease enables molecular and functional analysis of human cancer-associated mucins
Kissel et al. Surface immunoglobulin variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B-cell activation
Kissel Kissel, T.
CN120988133A (zh) 一种cd147纳米抗体d2、其制备方法与应用
Li et al. Recombinant Expression and Purification of Mouse Nectin-like 4 Glycoprotein in 293ET Cell Line
WO2020223328A1 (en) Compositions and methods for detection of disease-related antibody
Rizkallah Purification and characterization of N-acetylmuramyl-l-Alanine amidase from human plasma using monoclonal antibodies
NZ622172B2 (en) Endoglycosidase from streptococcus pyogenes and methods using it

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150513

Termination date: 20180911