CN102580129A - High-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium - Google Patents
High-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium Download PDFInfo
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- CN102580129A CN102580129A CN2012100447938A CN201210044793A CN102580129A CN 102580129 A CN102580129 A CN 102580129A CN 2012100447938 A CN2012100447938 A CN 2012100447938A CN 201210044793 A CN201210044793 A CN 201210044793A CN 102580129 A CN102580129 A CN 102580129A
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Abstract
The invention relates to the technical field of disinfection and sterilization of materials, and particularly relates to a high-pressure-free and short-term normal pressure sterilizing method for a cordyceps sinensis culture medium. The high-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium particularly comprises the following steps of: taking stable chlorine dioxide powder containing 10% of chlorine dioxide as a disinfectant, sterilizing nutrient solution and solid raw materials composed of cordyceps sinensis culture medium in the earlier stage, and carrying out short-term common pressure disinfection and sterilization on the sterilized cordyceps sinensis culture medium. By the virtue of the sterilizing method provided by the utility model, the cordyceps sinensis culture medium can be sterilized without high temperature and high pressure so as to effectively prevent the nutrition of the cordyceps sinensis culture medium from loosing. Even if the normal pressure disinfection and sterilization are carried out, the time of the disinfection and sterilization is short. According to the high-pressure-free and short-term normal pressure sterilizing method, the sterilization time is greatly reduced, and large quantity of energy such as electric energy, coal and fire wood can be obviously saved. By implementing the invention, the operating efficiency can be obviously improved, and meanwhile the operating cost is lowered, thus being worthy of being popularized.
Description
Technical field
The present invention relates to a kind of material disinfecting technical field, be specifically related to a kind of Cordyceps culture medium and exempt from high pressure and normal pressure disinfectant method in short-term.
Technical background
The Cordyceps culture medium mainly cooperates some organic substances of interpolation to form by all kinds of high protein materials such as rice, Semen Tritici aestivi, corns again.The culture medium nutrition of forming is very abundant, if culture medium without sterilization to aseptic condition, will mouldy easily, acidify, rotten, the cultivation Cordyceps also can be failed.
So in the Cordyceps production process, culture medium must just insert cordyceps species later on through the sterilization of strictness, just turns out grass by Routine Management then; Therefore, medium sterilization is the key of cultivation Cordyceps.
At present, the main two kinds of approach sterilization of Cordyceps culture medium, the one, high pressure, high-temperature sterilization sterilization, culture medium is at 1.5 kilograms/cm
2Pressure, temperature are to keep sterilization 2 hours under 121 ℃ the high pressure, hot conditions; The 2nd, the normal pressure sterilization, culture medium is under the normal pressure kettle temperature has reached 100 ℃ condition, to keep sterilization 8～10 hours.The equipment use cost of high pressure, high-temperature sterilization sterilization is big, and it is also big to consume energy; In addition, culture medium is in case through high pressure, high-temperature sterilization, and its nutritional labeling will run off to some extent.The infrastructure of normal pressure sterilization requires low, and is few through the culture medium nutrition leak of normal pressure sterilization; But the process of normal pressure sterilization is very long, and time-consuming effort again simultaneously also need consume a large amount of energy.
Summary of the invention
The objective of the invention is to overcome above technical deficiency and provide brand-new a kind of Cordyceps culture medium to exempt from high pressure and normal pressure disinfectant method in short-term.Specifically mainly be: utilize content be the stability chlorine dioxide powder of 10% chlorine dioxide as disinfectant, nutritional solution and solid feed that the Cordyceps culture medium is formed carry out the sterilization in early stage, again through in short-term normal pressure sterilization.
Chlorine dioxide described in the present invention; Be efficient, the wide spectrum of the latest generation of generally acknowledging in the world at present, the antibacterial of safety; Can under extremely low concentration (0.1ppm), kill the thalline of virus, antibacterial, protista, algae, fungus and various spore and Sporulation, developed country is widely used in the world.The Ministry of Chemical Industry of country has issued relevant industry standard, and health ministry approved chlorine dioxide is disinfectant and new food additive.
After chlorine dioxide adds the nutritional solution of Cordyceps culture medium according to quantity to; In 30 minutes, just accomplish nutritional solution sterilizing process; The Cordyceps culture medium raw material absorbs the nutritional solution with sterilizing function needs 4～5 hours, when absorbing nutritional solution, also raw material itself is carried out disinfection.Chlorine dioxide is killed function to harmful bacterium etc., can injure Cordyceps mycelium equally, but chlorine dioxide puts after 30 hours and through being heated to after temperature is higher than 60 ℃ through quiet, and the sterilizing ability of chlorine dioxide will complete obiteration; The Cordyceps culture medium is also will be through inserting cordyceps species again after the normal pressure sterilization under 100 ℃ of environment of 1 hour among the present invention, and then chlorine dioxide just can not have any injury to cordyceps species.
The present invention realizes through following technical scheme:
The Cordyceps culture medium is exempted from high pressure and is reached normal pressure disinfectant method in short-term, and the method step in the technical scheme is following:
1, using earlier content is that the stability chlorine dioxide powder of 10% chlorine dioxide is as Cordyceps culture medium raw material disinfectant in early stage.
2, the chlorine dioxide powder is to be added in the nutritional solution that has prepared, and chlorine dioxide powder addition is: it is the stability chlorine dioxide powder of 10% chlorine dioxide that the nutritional solution of 1000ml adds 1g content; Add after the chlorine dioxide powder, nutritional solution becomes the disinfectant solution of existing sterilizing function.
3, according to ratio culture medium raw material and the nutritional solution with sterilizing function are mixed in the Cordyceps culture vessel, culture vessel is sealed mouth and quiet putting 5～6 hours, culture medium raw material also carries out disinfection to raw material itself when absorbing nutritional solution.
4, will be move on to and carry out steaming and decocting in the normal pressure pot through the culture medium raw material of disinfection by chlorine dioxide, when the temperature in the normal pressure pot reach 100 ℃ the time keep sterilization 1 hour.
5, both can insert cordyceps species and grass is turned out in management through the culture medium raw material of normal-pressure sterilization again by routine.
Compared with prior art, the existing following advantage of the present invention:
1, production technology proposed by the invention is simple, easy to be implemented.
2, the present invention select for use disinfectant---the chlorine dioxide powder is cheap, and consumption is few during use.
3, embodiment of the present invention can realize that the Cordyceps culture medium need not pass through high temperature, autoclaving, has effectively kept the nutrient of culture medium not run off.
4, embodiment of the present invention can realize the normal-pressure sterilization sterilization in short-term of Cordyceps culture medium, reduces sterilization time significantly, also saves a large amount of electricity, coal, bavin equal energy source.
Also reduced operation cost when 5, embodiment of the present invention can obviously improve work efficiency, be worth promoting.
The specific embodiment
Below in conjunction with embodiment method of the present invention is further specified.
The Cordyceps culture medium is exempted from high pressure and is reached normal pressure disinfectant method in short-term, and the practical implementation method is following:
The Cordyceps culture medium is exempted from high pressure and is reached normal pressure disinfectant method in short-term, and the method step in the technical scheme is following:
1, using earlier content is that the stability chlorine dioxide powder of 10% chlorine dioxide is as Cordyceps culture medium raw material disinfectant in early stage.
2, the chlorine dioxide powder is to be added in the nutritional solution that has prepared, and chlorine dioxide powder addition is: it is the stability chlorine dioxide powder of 10% chlorine dioxide that the nutritional solution of 1000ml adds 1g content; Add after the chlorine dioxide powder, nutritional solution becomes the disinfectant solution of existing sterilizing function.
3, according to ratio culture medium raw material and the nutritional solution with sterilizing function are mixed in the Cordyceps culture vessel, culture vessel is sealed mouth and quiet putting 5～6 hours, culture medium raw material also carries out disinfection to raw material itself when absorbing nutritional solution.
4, will be move on to and carry out steaming and decocting in the normal pressure pot through the culture medium raw material of disinfection by chlorine dioxide, when the temperature in the normal pressure pot reach 100 ℃ the time keep sterilization 1 hour.
5, both can insert cordyceps species and grass is turned out in management through the culture medium raw material of normal-pressure sterilization again by routine.
Claims (1)
1. the Cordyceps culture medium is exempted from high pressure and is reached normal pressure disinfectant method in short-term, it is characterized in that method step is following:
(1) using earlier content is that the stability chlorine dioxide powder of 10% chlorine dioxide is as Cordyceps culture medium raw material disinfectant in early stage;
(2) the chlorine dioxide powder is to be added in the nutritional solution that has prepared, and chlorine dioxide powder addition is: it is the stability chlorine dioxide powder of 10% chlorine dioxide that the nutritional solution of 1000ml adds 1g content; Add after the chlorine dioxide powder, nutritional solution becomes the disinfectant solution of existing sterilizing function;
(3) according to ratio culture medium raw material and the nutritional solution with sterilizing function are mixed in the Cordyceps culture vessel, culture vessel is sealed mouth and quiet putting 5～6 hours, culture medium raw material also carries out disinfection to raw material itself when absorbing nutritional solution;
(4) will be move on to and carry out steaming and decocting in the normal pressure pot through the culture medium raw material of disinfection by chlorine dioxide, when the temperature in the normal pressure pot reach 100 ℃ the time keep sterilization 1 hour;
(5) both can insert cordyceps species and grass is turned out in management through the culture medium raw material of normal-pressure sterilization again by routine.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100447938A CN102580129A (en) | 2012-02-25 | 2012-02-25 | High-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium |
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| CN2012100447938A CN102580129A (en) | 2012-02-25 | 2012-02-25 | High-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392499A (en) * | 2013-06-22 | 2013-11-20 | 何寒 | Method for preparing edible fungus fruiting fungus bars from full raw materials directly |
| CN105363052A (en) * | 2015-12-10 | 2016-03-02 | 何寒 | Quick sterilization method of liquid culture medium for edible fungi |
| CN105886430A (en) * | 2016-04-27 | 2016-08-24 | 石家庄大众肥业有限公司 | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597924A (en) * | 2004-07-22 | 2005-03-23 | 深圳职业技术学院 | Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma |
-
2012
- 2012-02-25 CN CN2012100447938A patent/CN102580129A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597924A (en) * | 2004-07-22 | 2005-03-23 | 深圳职业技术学院 | Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392499A (en) * | 2013-06-22 | 2013-11-20 | 何寒 | Method for preparing edible fungus fruiting fungus bars from full raw materials directly |
| CN105363052A (en) * | 2015-12-10 | 2016-03-02 | 何寒 | Quick sterilization method of liquid culture medium for edible fungi |
| CN105886430A (en) * | 2016-04-27 | 2016-08-24 | 石家庄大众肥业有限公司 | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation |
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Application publication date: 20120718 |