CN102579697B - Chinese herbal prescription for treating coccidiosis in chicken - Google Patents
Chinese herbal prescription for treating coccidiosis in chicken Download PDFInfo
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Abstract
The invention discloses a Chinese herbal prescription for treating coccidiosis in chicken, which is prepared from sweet wormwood herb, antifeverile dichroa root, rhynohophylla, quisqualis indica, hairyvein agrimony, sanguisorba officinalis, rhizoma atractylodis, coptis, golden cypress, fructus gardeniae praeparatus, andrographis paniculata, philippine violet herb, dried orange peel and liquorice. The prescription has an obvious inhabiting effect on CVCC-245 bacterial strains of Escherichia coli; and coccidiosis ova artificial infection control experiments show that the effective rate is 94.17-99.17%, the field application and popularization tests show that the effective rate is 95.1%, and the application of the prescription has an obvious control effect and safety in use. The prescription has the efficacies of coccidiosis killing, hemostasis, dampness resolving, spleen invigorating and diarrhea relieving with astringents.
Description
Technical field
The present invention relates to a kind of Chinese herbal and crude drugs preparations for animals, particularly a kind of chicken coccidiosis Chinese herbal and crude drugs preparations for the treatment of belongs to the veterinary drug technical field.
Background technology
Chicken coccidiosis is comparatively normal a kind of opportunistic illnesses of sending out during poultry is produced, and it causes a disease and formerly is mainly tender eimeria tenella, murder by poisoning eimeria tenella ovum infects, and mostly occurs at the chicken house of high-temperature high humidity season and colony house humidity, place heavy contamination.According to its parasitic position difference, can be divided into small intestinal coccidiosis (murder by poisoning eimeria tenella) and caecum coccidiosis (tender eimeria tenella) clinically infects, in pathogenic process, often with pathogenic entero becteria secondary infection such as escherichia coli, Salmonella mutually and deposit, the chicken group is in different age in days sections, and all there are larger difference in the frequency of its generation, time and clinical disease performance under the different production environment conditions.When the caecum coccidiosis is taken place, show as mainly that the night blindness intestinal is acute bleeds profusely, draw cerise or red sauce loose stool, when secondary escherichia coli, Salmonella mixed infection, draw reddish yellow or loose stool red and white, as handle untimelyly, easily cause the acute large tracts of land death of chicken group; When the small intestinal coccidiosis is taken place, mainly show as chicken duodenum, acute, the gangrenous inflammation of small intestinal generation, the chicken all living creatures is long slow, gradual becoming thin and sporadic death occur.For a long time, antibiotic such as sulfonamides, Amprolium, Maduramicin and chemical medicine are this sick common drugs of control, have stronger drug resistance and antibiotic remains, and safety in production brings potential harm to food.In recent years, we use the Chinese herbal medicine screening technique that corresponding study on prevention has been carried out in the control of chickens infected with eimeria, intend seeking that toxic and side effects is little, residual hazard is low, be difficult for the Chinese herbal and crude drugs preparations for animals that produces drug resistance, have better curative effect.
Summary of the invention
The object of the present invention is to provide a kind ofly has the obvious suppression effect to chicken colibacillosis CVCC-245 pathogenic strain; It is 94.17～99.17% that chicken coccidiosis ovum artificial challenge prevents and treats the experiment effective percentage, and field application test effective percentage is 95.1%, and its application has the treatment chicken coccidiosis Chinese herbal and crude drugs preparations of remarkable prevention effect and safety in utilization.
The present invention is achieved in that the prescription composition for the treatment of chicken coccidiosis Chinese herbal and crude drugs preparations: Herba Artemisiae Annuae 40-80g, Radix Dichroae 30-70g, Ramulus Uncariae Cum Uncis 10-40g, Fructus Quisqualis 25-65g, Herba Agrimoniae 30-70g, burnt Radix Sanguisorbae 30-70g, Rhizoma Atractylodis 15-35g, Rhizoma Coptidis 15-35g, Cortex Phellodendri 20-40g, Fructus Gardeniae Preparatus 20-40g, punching connect 20-40g, Herba Violae 15-35g, Pericarpium Citri Reticulatae 20-40g, Radix Glycyrrhizae 10-30g.
The prescription of the treatment chicken coccidiosis Chinese herbal and crude drugs preparations after preferably, preferred is formed: Herba Artemisiae Annuae 60g, Radix Dichroae 50g, Ramulus Uncariae Cum Uncis 30g, Fructus Quisqualis 45g, Herba Agrimoniae 50g, burnt Radix Sanguisorbae 50g, Rhizoma Atractylodis 25g, Rhizoma Coptidis 25g, Cortex Phellodendri 30g, Fructus Gardeniae Preparatus 30g, punching connect 30g, Herba Violae 25g, Pericarpium Citri Reticulatae 30g, Radix Glycyrrhizae 20g.
With above crude drug shatter, 80 orders sieve, mix homogeneously, encapsulate airtight.Usage and consumption: usage: spice is freely adopted and is raised; Therapeutic dose: 500g spice 250kg; The preventive dose use that reduces by half.
Preparation of the present invention is mainly used in chicken coccidiosis ovum and infects and to cause so that the night blindness intestinal is acute and bleed profusely,
Draw the alternate color loose stool of cerise, red sauce or reddish yellow, and with small intestinal inflation, intestinal wall attenuation, to draw Fructus Lycopersici esculenti sample or chyme sample loose stool be the chicken coccidiosis of main clinical pathologic characteristic.Escherichia coli intestinal mixed infection to the while secondary also has the better prevention effect.This experimentation is through the tender eimeria tenella ovum of chicken infection morbidity treatment, external antibacterial, and series of experiment research such as the acute and chronic toxicity of laboratory animal medicine, physical and chemical determination, field application control test show: chicken colibacillosis CVCC-245 bacterial strain is had the obvious suppression effect; It is 94.17～99.17% that chicken coccidiosis ovum artificial challenge prevents and treats the experiment effective percentage, and field application test effective percentage is 95.1%.The control theory of Chinese veterinarian's medical science " treating both the principal and secondary aspects of a disease " is followed in its prescription medicine screening strictness, has fully taken into account the clinical pathology characteristic of chicken coccidiosis during poultry is produced, and its application has remarkable prevention effect and safety in utilization.Effect with parasite killing, hemostasis, removing dampness spleen invigorating, relieving diarrhea with astringents.
, test material
Preparation trade name of the present invention " the coccidiosis dysentery is loose ".
1.1 experiment medicine and prescription:
" the coccidiosis dysentery is loose " main ingredient of prescription: Herba Artemisiae Annuae, Radix Dichroae, Fructus Quisqualis, Herba Agrimoniae, burnt Radix Sanguisorbae, Rhizoma Atractylodis, Rhizoma Coptidis, Cortex Phellodendri, Fructus Gardeniae Preparatus, punching company, Herba Violae, Pericarpium Citri Reticulatae, Radix Glycyrrhizae etc.
1.2 experimental strain: chicken colibacillosis CVCC-245 bacterial strain is available from China Veterinery Drug Inspection Office; Tender eimeria tenella ovum is that Guizhou Farming Animal Science and Veterinary Research Institute original endoparasite research department preserves.
1.4 laboratory animal: 480 of 35 ages in days hybridization broiler, body weight 400g ± 25g
1.5 acute, sub-acute toxicity test animal: 40 of Kunming white mice (each 20 of male and females), 32 of SD rats (each 16 of male and females), laboratory animal room provides by Guiyang Medical College.
1.6 test with culture medium and reagent: meat soup, Yihong methylene blue, Mai Kangkai, saturated brine, 2.5% potassium dichromate solution.
, experiment content and method
2.1 antibacterial culturing: through 37 ℃ of 24h the CVCC245 bacterial strain is cultivated rejuvenation with Yihong methylene blue, Mai Kangkai selective medium, be inoculated in amplification culture in the broth bouillon.
2.2 external bacteriostatic experiment: preparation is decocted the drug level preparation that is condensed into 1g crude drug/ml make the annular antibacterial scraps of paper (diameter 3mm), carry out antibacterial cultivation 24h in ordinary flat, observe the growing state of CVCC-245 bacterial strain, be judged to effectively greater than 5～6mm as antibacterial circle diameter.
2.3 laboratory animal grouping: establish 4 of experimental grouies, i.e. 1 of prescription prevention group, 1 of prescription treatment group, 1 of chemical medicine treatment group, 1 of observation matched group; Every group of experimental chicken quantity is 120.
2.4 infection morbidity experiment
2.4.1 egg capsule is hatched: get tender eimeria tenella ovum 2.5% potassium dichromate solution, 28 ℃ of constant temperature culture stir 3～5 every day, when the egg capsule more than 80～85% is grown for the spore egg capsule, collect egg capsule and preserve standby.
2.4.2 humiture control: the experimental chicken group is given up interior temperature control remain on 25-28.5 ℃; Bedding and padding manually squirt, and humidity control remained on 75-80% in the experimental chicken group was given up.
2.4.3 infection morbidity criterion: the spore egg capsule liquid 500ml after will hatching joins 2～3 hours disposable drinking in each experimental group chicken group drinking-water by 1:30, through breeding observing, treating that cerise or red and white loose stool appear drawing in the chicken group, is experimental standard when the clinical onset rate reaches 12-15%.
2.4.4 chicken manure just coccidiosis ovum detects counting: use colony's sampling method to gather 50-60 chicken manure from each experimental group respectively, every group of chicken manure mixed respectively, it is standby to put 4 ℃ of preservations of refrigerator.Each group is got the 15g chicken manure, adds the 80 ml water back 60 order copper sieve that stirs respectively and filters, and moves into the 10ml centrifuge tube, 3000r/min centrifugation; In precipitate, fill it up with saturated brine, after the centrifugal mouth of pipe covers coverslip, leaves standstill floating 20min, take off coverslip and be placed on the microscope slide, put 100 times of microscopicallies and observe worm's ovum and counting.Counting is respectively organized 6 visuals field of excrement sample at random, gets the meansigma methods of egg capsule sum as infection intensity, and no egg capsule person is "-", 1～3 egg capsule person is "+", 4～6 egg capsule persons are " ++ ", and 8～12 above egg capsule persons are " +++", and 13 above egg capsule persons are " ++ ++ ".Each experimental group respectively before infection morbidity, before the control, control back detects counting once.
2.5 control experiment
2.5.1 medicine for treatment dosage and method: treat experimental chicken mass-sending after being ill, to first, second experimental group with " the coccidiosis dysentery is diffusing " Chinese herbal medicine powder (80 orders sieve) of preparation by every day per kilogram of body weight 2g spice freely adopt and raise, use 6d continuously; To the 3rd experimental group with 10% Embazin (Henan stablize the country pharmaceutcal corporation, Ltd produce) by every day 100g convert water 125 ㎏ and freely drink, be used in conjunction 6d; The 4th not medication of experimental group is for observing matched group.
2.4 acute, subacute toxicity pharmacological testing: according to country " Chinese medicine natural drug long term toxicity research guideline ", formulate acute toxicity test (MTD) and repetitively administered toxicity test with II level laboratory animal Kunming white mice, rat (SD strain), at utmost to expose its drug toxicity, to inspections such as its blood coagulation system index, blood parameters and each main device disease of ZANG-organs reason histology variations.
2.5 physical and chemical determination experiment: will " the coccidiosis dysentery is loose " Chinese herbal and crude drugs preparations entrust Institute of Analysis of Guizhou Normal University according to 5337-2008 JY/T023-1996 carry out main determination of heavy metal elements such as lead, cadmium, hydrargyrum, arsenic, copper.
2.6 field application test:: production prevention and the clinical treatment application test of the big coccidiosis of chicken carried out in the part chicken farm in areas such as Kweiyang, Guizhou Province, Zun Yi, the south of Guizhou Province, the southeast of Guizhou Province, Anshun, six Pan Shui.
, experimental result:
3.1 bacteriostatic experiment: antibacterial circle diameter is all between 4.8～6.6mm.
3.2 medical treatment experiment: the results are shown in Table 1.
Subordinate list 1:
Annotate: prescription prevention group is earlier with the laggard pedestrian worker's infection experiment of experiment prescription Prevention Processing 7d.
3.3 acute, subacute toxicity pharmacological testing:
3.4 physical and chemical determination analysis: see Table 2.
Subordinate list 2:
3.5 application test: the results are shown in Table 3.
Subordinate list 3:
4, experimentation analysis:
4.1 the Chinese medicine formula that this experimentation filters out " the coccidiosis dysentery is loose " has the effect of parasite killing, hemostasis, removing dampness spleen invigorating, relieving diarrhea with astringents.The external bacteriostatic experiment of chicken colibacillosis CVCC-245 bacterial strain shows that " the coccidiosis dysentery is loose " has the obvious suppression effect to chicken colibacillosis; The clinical prevention application test shows that this prescription has remarkable prevention effect to chicken coccidiosis, and it is 94.17～99.17% that its artificial challenge prevents and treats the experiment effective percentage, 25.25 ten thousand of field application test chicken groups, average effectiveness level 95.1%.Wherein, test 1 group of sickness rate and cure rate and all significantly be better than other experimental group, show that " the coccidiosis dysentery is loose " has good early prevention and treatment effect to chickens infected with eimeria.
4.2 acute and sub-acute toxicity experimentation shows that " the coccidiosis dysentery is loose " no obvious toxicology meaning does not have obvious toxic and side effects in production application, have good safety in utilization and reliability.
4.3 the treatment use amount of " the coccidiosis dysentery is loose " is per kilogram of body weight 0.3g every day, using method is spice, be 1000g spice 250kg, the every 1000g feedstuff of content that increases behind the main heavy metal ion batch mixing in the physical and chemical determination is: arsenic 0.0107mg, plumbous 0.0314mg, hydrargyrum 0.0001mg, diaphragm 0.0011mg.The content of relevant heavy metal ion in China's feed safety production standard, that is: 0mg, lead≤13.0mg, hydrargyrum≤0.10mg, the diaphragm≤0.50mg of arsenic in every 1000g feedstuff≤2..Simultaneously, be 5～7d the service time of preventing and treating in the process, is difficult for causing the long-term cumulative action of main heavy metal ion.
4.4 in the poultry production practices, the generation of chicken coccidiosis and feeding environment have close dependency, often with pathogenic entero becteria secondary infection such as escherichia coli, Salmonella mutually and deposit, drug screening in " the coccidiosis dysentery is loose " prescription had both taken into full account the reliable effect of its parasite killing hemostasis, take into account the inhibitory action of right pathogenic entero becteria again, meet regularty of epidemic and the clinicopathological characteristics of current coccidiosis fully.
The specific embodiment
The embodiment of the invention 1: Herba Artemisiae Annuae 60g, Radix Dichroae 50g, Ramulus Uncariae Cum Uncis 30g, Fructus Quisqualis 45g, Herba Agrimoniae 50g, burnt Radix Sanguisorbae 50g, Rhizoma Atractylodis 25g, Rhizoma Coptidis 25g, Cortex Phellodendri 30g, Fructus Gardeniae Preparatus 30g, punching connect 30g, Herba Violae 25g, Pericarpium Citri Reticulatae 30g, Radix Glycyrrhizae 20g.
With above crude drug shatter, 80 orders sieve, mix homogeneously, encapsulate airtight.Usage and consumption: usage: spice is freely adopted and is raised; Therapeutic dose: 500g spice 250kg; The preventive dose use that reduces by half.
The embodiment of the invention 2: Herba Artemisiae Annuae 40g, Radix Dichroae 30g, Ramulus Uncariae Cum Uncis 10g, Fructus Quisqualis 25g, Herba Agrimoniae 30g, burnt Radix Sanguisorbae 30g, Rhizoma Atractylodis 15g, Rhizoma Coptidis 15g, Cortex Phellodendri 20g, Fructus Gardeniae Preparatus 20g, punching connect 20g, Herba Violae 15g, Pericarpium Citri Reticulatae 20g, Radix Glycyrrhizae 10g.
With above crude drug shatter, 80 orders sieve, mix homogeneously, encapsulate airtight.Usage and consumption: usage: spice is freely adopted and is raised; Therapeutic dose: 500g spice 250kg; The preventive dose use that reduces by half.
The embodiment of the invention 3: Herba Artemisiae Annuae 80g, Radix Dichroae 70g, Ramulus Uncariae Cum Uncis 40g, Fructus Quisqualis 65g, Herba Agrimoniae 70g, burnt Radix Sanguisorbae 70g, Rhizoma Atractylodis 35g, Rhizoma Coptidis 35g, Cortex Phellodendri 40g, Fructus Gardeniae Preparatus 40g, punching connect 40g, Herba Violae 35g, Pericarpium Citri Reticulatae 40g, Radix Glycyrrhizae 30g.
With above crude drug shatter, 80 orders sieve, mix homogeneously, encapsulate airtight.Usage and consumption: usage: spice is freely adopted and is raised; Therapeutic dose: 500g spice 250kg; The preventive dose use that reduces by half.
Claims (2)
1. treatment
ChickenThe coccidiosis Chinese herbal and crude drugs preparations is characterized in that: it is made up of following crude drug: Herba Artemisiae Annuae 40-80g, Radix Dichroae 30-70g, Ramulus Uncariae Cum Uncis 10-40g, Fructus Quisqualis 25-65g, Herba Agrimoniae 30-70g, burnt Radix Sanguisorbae 30-70g, Rhizoma Atractylodis 15-35g, Rhizoma Coptidis 15-35g, Cortex Phellodendri 20-40g, Fructus Gardeniae Preparatus 20-40g, Herba Andrographis 20-40g, Herba Violae 15-35g, Pericarpium Citri Reticulatae 20-40g, Radix Glycyrrhizae 10-30g.
2. a kind for the treatment of according to claim 1
ChickenThe coccidiosis Chinese herbal and crude drugs preparations is characterized in that: it is made up of following crude drug: Herba Artemisiae Annuae 60g, Radix Dichroae 50g, Ramulus Uncariae Cum Uncis 30g, Fructus Quisqualis 45g, Herba Agrimoniae 50g, burnt Radix Sanguisorbae 50g, Rhizoma Atractylodis 25g, Rhizoma Coptidis 25g, Cortex Phellodendri 30g, Fructus Gardeniae Preparatus 30g, Herba Andrographis 30g, Herba Violae 25g, Pericarpium Citri Reticulatae 30g, Radix Glycyrrhizae 20g.
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| CN102895446B (en) * | 2012-10-30 | 2014-08-13 | 天津必佳药业有限公司 | Traditional Chinese medical composition for treatment of toxocara canis diseasees |
| CN103142771B (en) * | 2013-04-02 | 2014-09-03 | 河北工程大学 | Medicine for controlling coccidiosis in chicken |
| CN104547375A (en) * | 2014-12-16 | 2015-04-29 | 北京农学院 | Eimeria tenella resistant traditional Chinese medicine prescription taking atractylodes lancea as main component |
| CN104922586B (en) * | 2015-01-19 | 2018-05-29 | 山东宝来利来生物工程股份有限公司 | A kind of preparation and its application of the probiotics fermention Chinese medicine preparation for preventing chicken coccidiasis |
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| CN101007126A (en) * | 2007-01-23 | 2007-08-01 | 天津生机集团有限公司 | Drug for preventing or treating parasites of avian and its preparation method |
| CN101209296A (en) * | 2006-12-25 | 2008-07-02 | 天津市润拓生物技术有限公司 | Chinese medicinal granule with worm-expelling liver-caring, hemostasis and dysentery-stopping efficacy |
| CN101297941A (en) * | 2008-06-16 | 2008-11-05 | 贺州学院 | Combined Chinese and western medicine sustained-release agent for preventing and treating rabbit coccidiosis and preparation method thereof |
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| CN101209296A (en) * | 2006-12-25 | 2008-07-02 | 天津市润拓生物技术有限公司 | Chinese medicinal granule with worm-expelling liver-caring, hemostasis and dysentery-stopping efficacy |
| CN101007126A (en) * | 2007-01-23 | 2007-08-01 | 天津生机集团有限公司 | Drug for preventing or treating parasites of avian and its preparation method |
| CN101297941A (en) * | 2008-06-16 | 2008-11-05 | 贺州学院 | Combined Chinese and western medicine sustained-release agent for preventing and treating rabbit coccidiosis and preparation method thereof |
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