CN101797289A - Oral spraying agent prepared from traditional Chinese medicine and preparation method thereof - Google Patents
Oral spraying agent prepared from traditional Chinese medicine and preparation method thereof Download PDFInfo
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- CN101797289A CN101797289A CN201010140223A CN201010140223A CN101797289A CN 101797289 A CN101797289 A CN 101797289A CN 201010140223 A CN201010140223 A CN 201010140223A CN 201010140223 A CN201010140223 A CN 201010140223A CN 101797289 A CN101797289 A CN 101797289A
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- rhizoma coptidis
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Abstract
The invention relates to a spraying agent prepared from a traditional Chinese medicine for inhibiting oral bacteria, treating dental ulcer and preventing and treating dental caries, which comprises the components in parts by weight: 10-20 parts of gallnut and coptis extract, 10-20 parts of tween-80/glycerol/acacia/gelatin, 10-20 parts of span-80, 60-70 parts of simple syrup and 800-900 parts of purified water. The gallnut and coptis extract has the following extracting steps: crushing gallnut and coptis and then passing through a sieve with 80 meshes, wherein the mass ratio of the gallnut to the coptis is (1:6)-(1:12); extracting with ethanol solution with the volume ratio of 40-80 percent; and filtering and drying the extracted filtrate. The invention has the advantages of simple preparation method, convenient use, pure traditional Chinese medicine, no addition of any antibiotic and no generation of bacterial drug resistance; and in addition, the invention can be directly used for mucosa inside an oral cavity as well as used for inhibiting oral bacteria, treating the dental ulcer and preventing and treating dental caries.
Description
Technical field
The present invention relates to a kind ofly to suppress oral cavity bacterium, treat oral ulcer, prevent and treat medicinal herb spray of dental caries and preparation method thereof.
Background technology
Contain a large amount of antibacterials in the human oral cavity, as streptococcus, staphylococcus, bacillus pyocyaneus, escherichia coli etc.When the balance of these floras is broken, will cause multiple oral disease, as oral ulcer, dental caries etc., have a strong impact on the healthy of people.
Oral ulcer claims that again (Recurrent Oral Ulcer ROU), is a kind of more common disease of cari oris mucosa to recurrent aphtha, and its reason complexity is not illustrated as yet fully, mostly is antibacterial or viral infection causes.High and the very easily recurrence of oral ulcer sickness rate, the sense that has an intense pain during outbreak, influence feed and speaking brings huge painfully and inconvenient to the patient, and when particularly meeting acid, one-tenth, peppery food, pain is severity more.Though be the little disease in oral cavity, painfully can't bear, even feel restless, worried waking or sleeping, depressed.The prolonged ulcer that does not heal in the oral cavity is because canceration with the passing of time also might take place in the stimulation of often being chewed, speaking.
Dental caries is another big disease of harm humans oral health.Modern cariology thinks that dental caries is flora disruption of ecological balance disease in itself.The cariogenic bacteria abnormal proportion increases in dental plaque, and the probiotics abnormal proportion is when reducing, can cause dental plaque and be transformed to the cariogenicity bacterial plaque by non-cariogenicity bacterial plaque, cariogenic bacteria is carrying out complicated biochemical metabolism in bacterial plaque, utilize exogenous carbohydrate metabolism to produce various extracellular polysaccharide, promote various cariogenic bacterias to gather in the adhesion of facing, and and then produce organic acid, when cumulative acid reaches critical pH, i.e. the acid etching demineralization of visible dental hard tissue, Organic substance decomposes, dental caries thereby generation.Therefore domestic and international in recent years many scholars are devoted to seek effectively to keep oral cavity flora microecological balance medicine, treat or the generation of caries prevention.
Make a general survey of the various medicines that are used for the treatment of oral ulcer at present, various in style, from dosage form, because the therapeutic domain of membrane, tablet, powder, unguentum is all more limited, be at ulcer wound surface, and spray can finely address this problem, and not only can treat ulcer surface, other position floras of all right balance oral cavity play the effect of prevention.Existing oral spray ingredient is comparatively complicated on the market, and expensive, majority contains antibiotic, easily causes bacterial drug resistance, and resistant rate of antibacterial and the recall rate of antibacterial are ascendant trend year by year, and the exploitation of new antibiotic and chemicals can not be satisfied market demand far away.
Summary of the invention
The purpose of this invention is to provide and a kind ofly suppress oral cavity bacterium, treat oral ulcer, prevent and treat dental caries, preparation method is simple, easy to use, cheap, do not contain antibiotic pure Chinese medicine oral spray.
Another purpose of the present invention provides the preparation method of above-mentioned oral spray.
Implementation procedure of the present invention is as follows:
A kind of oral spray, its composition and weight portion are:
10～20 parts of Galla Chinensis Rhizoma Coptidis extracts
10～20 parts of tween 80/glycerol/arabic gum/gelatin
10～20 parts of Arlacel-80s
60～70 parts of simple syrups
800～900 parts of pure water
2～4 parts of antiseptic
3～7 parts of fruity flavors
Galla Chinensis Rhizoma Coptidis extract leaching process is: Galla Chinensis, Rhizoma Coptidis pulverizing medicinal materials are crossed 80 mesh sieves, and the two mass ratio is (1: 6)～(1: 12), are that 40%～80% alcoholic solution extracts with volume ratio, extracting liquid filtering, dry getting final product.
Above-mentioned Chinese medicine oral cavity spray preparing process comprises the steps:
A, add 10～20 parts of tween 80/glycerol/arabic gum/gelatin and 10～20 parts of Arlacel-80s in 10～20 parts of Galla Chinensis Rhizoma Coptidis extracts, ground and mixed is even;
B, 800～900 parts of pure water of adding continue ground and mixed;
C, in said mixture, add 60～70 parts of simple syrups, 3 parts of antiseptic, 5 parts of fruity flavors at room temperature mix and stir, and fill promptly gets this Chinese medicine oral spray.
The Rhizoma Coptidis of using among the present invention (Coptis chinensis) medical material, it is a kind of Chinese medicine, its effective ingredient is berberine (Berberine), it is the isoquinoline alkaloid that from plants such as Rhizoma Coptidis, extracts, cold in nature, bitter in the mouth, can eliminating fire and detoxication, experiment in vitro finds that it all has inhibition or killing action to bacillus pyocyaneus, gonococcus and Fu Shi, dysentery bacterium, simultaneously virus, protozoon, nematicide are also had and suppress or killing action, all right enhancing non-specific immunity is the main component that suppresses oral cavity bacterium.
Its main component of Galla Chinensis (Chinese gal) is tannin (Gallicum), and content is up to more than 70%.Gallotannin has precipitation to protein, and behind skin, mucosa, the ulcer contact tannic acid, its histone is solidified, and forms one deck tunicle, is astriction; The also oppressed contraction of little blood vessel simultaneously, blood clotting and be hemostasia effect; The protein of glandular cell is solidified and causes that secretion suppresses, and produces the mucosa drying; The teleneuron protein precipitation can be faint local anesthesia phenomenon, and ulcer surface is had very significant effect.
Because berberine is a kind of alkaloid, taste is very bitter, is not suitable for being used for separately making oral spray.And tannin has acidity, and inlet has very strong astringent taste, and zest is stronger, also is not suitable for being used for separately making oral spray.In view of the two separately chemical property and the characteristics on the taste, the inventor prepares the two mixed extract, experiment showed, that the mixed extract of the two both can suppress oral cavity bacterium, can fine promotion healing effect be arranged to ulcer surface again.
Advantage of the present invention and good effect:
Chinese medicine oral spray preparation method of the present invention is simple, easy to use, pure Chinese medicinal preparation, do not add any antibiotic, can not cause the generation of bacterial drug resistance, but human body immunity improving power, enlarge the drug effect scope, also can control and to prevent, use side effect little, safe and reliable.This oral spray can be directly used in the inner mucosa in oral cavity, can suppress oral cavity bacterium, treats oral ulcer, prevent and treat dental caries, eliminates the inducement of causing a disease, and promotes body health, alleviates local symptom, promotes ulcer healing.
The specific embodiment
For a more clear understanding of the present invention and the drug effect of medicament of the present invention, below the present invention is described in further detail by specific embodiment.
The optimum extracting method of embodiment 1 Galla Chinensis Rhizoma Coptidis extract effective ingredient
According to the extracting method and the dosage form needs of traditional decoction, ethanol content, supersound process time, Galla Chinensis Rhizoma Coptidis quality of medicinal material serve as to investigate index than being the investigation factor with drying precipitated amount in the selected extracting solution, select L for use
9(3)
4Orthogonal table is tested.
Table 1 factor level table
The intuitive analysis of result of the test and ANOVA showed significant, the factor that effective component extracting is had the greatest impact is an ethanol content, optimised process is A
1B
2C
1, promptly ethanol content 50%, supersound process 20min, and Galla Chinensis, Rhizoma Coptidis mass ratio are 1: 8.
Tween 80/glycerol/arabic gum/gelatin and Arlacel-80 are best by 1: 1 o'clock effect.
Embodiment 2
A kind of oral spray, its composition and weight portion are:
20 parts of Galla Chinensis Rhizoma Coptidis extracts
20 parts of tween 80s
20 parts of Arlacel-80s
70 parts of simple syrups
862 parts of pure water
3 parts of antiseptic
5 parts of fruity flavors
Above-mentioned Galla Chinensis Rhizoma Coptidis extract leaching process is: Galla Chinensis, Rhizoma Coptidis medical material pulverize separately, cross 80 mesh sieves, and the two mass ratio is 1: 8, is 50% alcoholic solution supersound extraction three times with volume ratio respectively, each 20 minutes, solid-liquid ratio was 1: 10.The extracting solution of the two is mixed, filter dry 12h below 60 ℃ in baking oven.
The low performance that presses down of embodiment 3 Galla Chinensis Rhizoma Coptidis extracts
Measuring the minimum inhibitory concentration of Galla Chinensis Rhizoma Coptidis extract, is solvent with DMF (N, dinethylformamide), is mixed with Concentraton gradient.
One, time and Test condition
In June, 2009 is at clinical laboratory of The People's Hospital Bacteriology Room.
Two, strain
Clinical laboratory of The People's Hospital Bacteriology Room provides a clinical staphylococcus epidermidis strain, and type strain 4 strains are purchased in Beijing biological study institute, are respectively escherichia coli, bacillus pyocyaneus, staphylococcus aureus, streptococcus albus.
Three, test and result evaluation method
Utilize drug susceptibility experiment screening sensitive strain, adopt the K-B agar diffusion method.The medicament sensitivity test reference standard is that Shanghai City health and epidemic prevention station standard operating procedure (method of inspection fascicle) is carried out with reference to agar dilution in the in-vitro antibacterial test method, with the type strain is contrast, when blank group, group of solvents well-grown, other plates raise with drug level, when the strain growth gradient is suppressed, prove Success in Experiment.
Four, result
37 ℃ of cultivations are after at least 24 hours, the perusal plate, no bacterial strain growth be 100% to be suppressed.At positive control pipe antibacterial normal growth, the negative control pipe does not have under the condition of bacterial growth, is minimum inhibitory concentration (MIC) with the concentration of the extract solution in vitro of no bacterial growth dilution.The geometric mean of more than testing 3 times is the final MIC value of medicine.
The minimum inhibitory concentration value of table 2 extract
Annotate: "-" is asepsis growth in the table, and "+" for bacteria growing is arranged, " (+) " has bacteria growing for continuing to cultivate 48h
Five, conclusion
Show by bacteriostatic test, the Galla Chinensis Rhizoma Coptidis extract to the pathogenic bacterium of common the caused oral ulcer in oral cavity, dental caries external all have suppress effect preferably.
Embodiment 4
One, time and Test condition
In October, 2009 is at life sciences institute of Northwest University Animal House.
Two, the foundation of artificial oral ulcer
Select each three of two groups of SD rats of A, B for use, use etherization, retract upper lip, expose the lip mucosa, with prefabricated diameter be the filter paper of 4mm, thick about 1mm to soak into volume fraction be 40% glacial acetic acid, place rat upper lip both sides, take out behind the 60s and see that promptly there is the white infringement of diameter 5mm in this district; The ulcer that diameter is about 5mm all appears in 48h.Surface-coated pseudomembrane, border are comparatively clear, congestion and edema on every side, and each ulcer surface size, shape and the degree of depth all connect closely, help testing the comparison of medication; Light microscopic and electron microscopic observation ulcer model, the mucosal epithelium dissolving comes off, the surface coverage slough, a large amount of cell infiltration under the ulcer, its pathological characteristic is similar substantially to human recurrent oral ulceration, shows that this Success in Experiment prepares animal stomatocace model.
Three, process of the test
For ulcerated two groups of SD rats, the A group: after early, middle and late feed every day, the drinking-water, the normal saline flushing ulcer surface, 0.2g Galla Chinensis Rhizoma Coptidis extract is applied to ulcer surface, forced closed oral cavity 5min, after put back to mouse cage, recover normal feed, drinking-water behind the 1h.The B group: after early, middle and late feed every day, the drinking-water, the normal saline flushing ulcer surface recovers normal feed, drinking-water behind the 1h.
Four, result of the test
Note observing the ulcer surface situation of change before each medication, the A group: behind the medication 12h, find before ulcer surface is than medication smooth.Behind the medication 36h, the ulcer surface border thickens unclear.Behind the medication 48h, ulcer surface begins healing.Behind the medication 72h-96h, ulcer surface all heals.The B group: the ulcer surface border thickens behind the 72h, during to 96h, and healing not yet.
Five, conclusion
This experiment shows that the Galla Chinensis Rhizoma Coptidis extract has the effect that promotes healing to oral ulcer.
Embodiment 5
One, time and Test condition
In November, 2009 is at life sciences institute of Northwest University Animal House.
Two, the foundation of artificial oral ulcer
Select each three of two groups of rabbit of A, B for use, 3% pentobarbital sodium rabbit ear edge intravenous anesthesia, retract upper lip, expose the lip mucosa, with prefabricated diameter be the filter paper of 5mm, thick about 1mm to soak into volume fraction be 40% glacial acetic acid, place rabbit upper lip both sides, take out behind the 60s and see that promptly there is the white infringement of diameter 5.5mm in this district; The ulcer that diameter is about 5.5mm all appears in 48h.Surface-coated pseudomembrane, border are comparatively clear, congestion and edema on every side, and each ulcer surface size, shape and the degree of depth all connect closely, help testing the comparison of medication; Light microscopic and electron microscopic observation ulcer model, the mucosal epithelium dissolving comes off, the surface coverage slough, a large amount of cell infiltration under the ulcer, its pathological characteristic is similar substantially to human recurrent oral ulceration, shows that this Success in Experiment prepares animal stomatocace model.
Three, process of the test
For ulcerated rabbit, A group: after early, middle and late feed every day, the drinking-water, the normal saline flushing ulcer surface, medical absorbent cotton is dried, and this spray is sprayed on ulcer surface, forced closed oral cavity 10min, after put back to rabbit-hutch, recover normal feed, drinking-water behind the 1h.The B group: after early, middle and late feed every day, the drinking-water, the normal saline flushing ulcer surface recovers normal feed, drinking-water behind the 1h.
Four, result of the test
Note observing the ulcer surface situation of change before each medication.A group: behind the medication 20h, find before ulcer surface is than medication smooth.Behind the medication 48h, the ulcer surface border thickens unclear.Behind the medication 60h, ulcer surface begins healing.Behind the medication 96h, ulcer surface all heals.The B group: when 96h, ulcer surface does not heal yet.
Five, conclusion
This experiment shows that Galla Chinensis Rhizoma Coptidis spray has the effect that promotes healing to oral ulcer.
Claims (6)
1. an oral spray comprises following component, and its weight portion is:
10～20 parts of Galla Chinensis Rhizoma Coptidis extracts
10～20 parts of tween 80/glycerol/arabic gum/gelatin
10～20 parts of Arlacel-80s
60～70 parts of simple syrups
800～900 parts of pure water.
2. oral spray according to claim 1, it is characterized in that the Galla Chinensis Rhizoma Coptidis extract adopts following method to obtain: Galla Chinensis, Rhizoma Coptidis pulverizing medicinal materials are crossed 80 mesh sieves, the two mass ratio is (1: 6)～(1: 12), with volume ratio is that 40%～80% alcoholic solution extracts extracting liquid filtering, drying.
3. oral spray according to claim 1 is characterized in that also containing 2～4 portions of antiseptic.
4. oral spray according to claim 1 is characterized in that also containing 3～7 parts of fruity flavors.
5. the described Chinese medicine of claim 1 oral cavity spray preparing process comprises the steps:
(1) add 10～20 parts of tween 80/glycerol/arabic gum/gelatin and 10～20 parts of Arlacel-80s in 10～20 parts of Galla Chinensis Rhizoma Coptidis extracts, ground and mixed is even;
(2) add 800～900 parts of pure water, continue ground and mixed;
(3) in said mixture, add 60～70 parts of simple syrups, mix to stir promptly getting this Chinese medicine oral spray.
6. the described oral spray of claim 1 is in treatment with prevent oral ulcer, prevent and treat the application in the dental caries.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010140223A CN101797289A (en) | 2010-04-07 | 2010-04-07 | Oral spraying agent prepared from traditional Chinese medicine and preparation method thereof |
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| CN201010140223A CN101797289A (en) | 2010-04-07 | 2010-04-07 | Oral spraying agent prepared from traditional Chinese medicine and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251672A (en) * | 2013-05-30 | 2013-08-21 | 成都大学 | Horseradish oral spray and preparation method thereof |
| CN106511471A (en) * | 2016-11-30 | 2017-03-22 | 浙江省林业科学研究院 | Fig oral cavity spray and preparation method thereof |
| CN110897985A (en) * | 2019-12-16 | 2020-03-24 | 广东盐业健康产业发展有限公司 | Oral spray formula |
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| CN1515255A (en) * | 2003-01-05 | 2004-07-28 | 山东绿叶天然药物研究开发有限公司 | Tannic acid berberine lozenge, its preparation method and application |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251672A (en) * | 2013-05-30 | 2013-08-21 | 成都大学 | Horseradish oral spray and preparation method thereof |
| CN106511471A (en) * | 2016-11-30 | 2017-03-22 | 浙江省林业科学研究院 | Fig oral cavity spray and preparation method thereof |
| CN106511471B (en) * | 2016-11-30 | 2019-11-22 | 浙江省林业科学研究院 | A kind of fig oral spray and preparation method thereof |
| CN110897985A (en) * | 2019-12-16 | 2020-03-24 | 广东盐业健康产业发展有限公司 | Oral spray formula |
| CN110897985B (en) * | 2019-12-16 | 2021-12-17 | 广东盐业健康产业发展有限公司 | Oral spray composition |
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Application publication date: 20100811 |