CN102557767A - Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof - Google Patents
Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof Download PDFInfo
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- CN102557767A CN102557767A CN2011104247141A CN201110424714A CN102557767A CN 102557767 A CN102557767 A CN 102557767A CN 2011104247141 A CN2011104247141 A CN 2011104247141A CN 201110424714 A CN201110424714 A CN 201110424714A CN 102557767 A CN102557767 A CN 102557767A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a special compound fungus agent for humification of fungus residues of straw mushrooms. The special compound fungus agent comprises fungus powder A and fungus powder B, wherein the fungus powder A is used in a retting period of the fungus residues of the straw mushroom; and the fungus powder B serves as auxiliary probiotic to be added into the retted fungus residues of the straw mushroom. The special fungus powder A and the special fungus powder B for the fungus residues of the straw mushroom are respectively used as zymocyte of the fungus residues of the straw mushroom and the auxiliary probiotic used after fermentation; by using the compound fungus agent, the retting cycle of the fungus residues can be obviously shortened, and the degradation of macro molecular substances comprising lignin, cellulose, hemicelluloses, and the like which are difficult to be utilized by plants and animal can be quickened in the retting process, so that after the fungus residues are fermented, the nutrient substances of the fungus residues can be utilized by the animals and the plants more quickly and more thoroughly; and through using the fungus powder B, the amount of nitrogen-fixing microorganisms, phosphate-solubilizing microorganisms and potassium-solubilizing microorganisms in the fungus residues are increased, the utilization rates of inorganic nitrogen, phosphate and potassium are increased, the use amount of chemical fertilizers is reduced, the cost is saved and the environment is protected.
Description
Technical field
The present invention relates to a kind of straw mushroom bacterium slag humification complex bacterial agent special and preparation method thereof.
Background technology
The bacterium slag is the culture medium waste that is left behind the results product in the edible fungus culturing process.Be rich in xylogen, Mierocrystalline cellulose etc. in the cultivating materials of edible mushrooms, in the process of growth of edible mushrooms, these nutritive substances are broken down into carbohydrate and carboritride etc., and a part is absorbed by mycelia, and some remains in the bacterium slag.After edible fungus culturing finished, also residual a large amount of mycelium in the bacterium slag can be used as good flower culture fertilizer or directly is used as culture substrate.If what the science of not doing was handled arbitrarily abandons the bacterium slag, not only waste resource and also cause mould and insect to grow easily, bring the serious environmental pollution problem.
Along with the fast development of pollution-free food, green food and Organic food, utilize agricultural wastes to produce the developing direction that organic fertilizer has become organic fertilizer.China is Edible Fungi big country, the annual bacterium slag that produces more than 6,000,000 tons, and the bacterium slag not only contains a large amount of nutritive substances, and deals with improperly also and can cause serious environmental pollution, so the reasonable disposal of edible fungi residues becomes a urgent problem.At present the treatment process to edible fungi residues has: secondary kind mushroom, soilless culture substrate, processing edible fungi residue feed, processing biological organic fertilizer, as fuel etc.Nutritive substance in this research combination straw mushroom bacterium slag and the biological characteristics of mikrobe; Developing a kind of can filling in the degraded straw mushroom bacterium slag is difficult for by the complex bacterial agent special of the nutritive substance of plant utilization; Fundamentally solve the low shortcoming of straw mushroom bacterium slag bioavailability; Shorten that it is innoxious, the time of recycling treatment, improve compost quality.
Summary of the invention
The present invention provides a kind of straw mushroom bacterium slag humification complex bacterial agent special and preparation method thereof.
Straw mushroom bacterium slag humification complex bacterial agent special comprises bacterium powder A, bacterium powder B; Bacterium powder A is used for the stack retting stage of straw mushroom bacterium slag, and bacterium powder B is as the straw mushroom bacterium slag of assisting after probiotic bacterium is added stack retting to.Bacterium powder A, bacterium powder B making method are following:
Bacterium powder A: will cultivate 14～16h, cell concentration is 10
7～10
8The Pseudomonas stutzeri ACCC 01977 (ACCC of CFU//mL; Chinese agriculture microbial strains preservation administrative center), subtilis ACCC 11089, the flat lead fungi ACCC 30414 of yellow spore mixed in 1: 1: 1 by volume; After the mixing, insert in the aseptic liquid nutrient medium by inoculum size 10% (V/V).Culture medium condition (w/v): 3% dregs of beans, 1.5% Semen Maydis powder saccharification liquid (v/v), 2% glucose, 0.3%KH
2PO
4, 0.15%MgSO
4, 37 ℃ of pH7.0, leavening temperature, mixing speed 120r/min, dissolved oxygen 20%; Bacterium liquid filters through 80 order strainers after cultivating 36h; In filtrating, add the porous-starch of filtrating weight 13%～16%; The maltodextrin and 0.1% Povidone, USP/EP of filtrating weight 14～16%, spray-dried after, add filtrating weight 4% superphosphate of lime and do weighting agent.Colony counting method detects the bacterium number, and Pseudomonas stutzeri ACCC01977, subtilis ACCC11089, flat lead fungi ACCC 30414 colony counts alive of yellow spore are respectively: 1.3～3.3 * 10
10CFU/g, 5.9～9..1 * 10
10CFU/g, 1.8～4.5 * 10
11CFU/g is bacterium powder A with the vacuum packaging of gained bacterium powder.
Bacterium powder B is made up of bacterium powder 1, bacterium powder 2.
Bacterium powder 1: will cultivate 12～16h, cell concentration is 10
7～10
8Bacillus cereus CGMCC1.2223 (the CGMCC of CFU//mL; China common micro-organisms culture presevation administrative center) 1: 10 by volume ratio of bacterium liquid is inoculated in the aseptic liquid nutrient medium; Culture medium condition (w/v): 1% Semen Maydis powder saccharification liquid (v/v); 37 ℃ of 1% peptones, 0.3% Carnis Bovis seu Bubali cream, 0.02%NaCl, pH7.0～7.2, leavening temperature, mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 36h; The porous-starch that in filtrating, adds filtrating weight 13%～16% respectively; 14～16% maltodextrin and 0.1% Povidone, USP/EP; After the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, be bacillus cereus CGMCC 1.2223 microbial inoculums.Colony counting method detects the bacterium number, and getting the Bacillus circulans viable count is 3.7～6.9 * 10
10CFU/g.
Bacterium powder 2: will cultivate 12～16h, cell concentration is 10
7～10
8Aztobacter sp CGMCC1.233 (the CGMCC of CFU//mL; China common micro-organisms culture presevation administrative center) 1: 10 by volume ratio of bacterium liquid is inoculated in the aseptic liquid nutrient medium; Culture medium condition (w/v): 0.05% yeast powder, 0.2% N.F,USP MANNITOL, 0.02%KH
2PO
4, 0.02%MgSO
4, 0.08%K
2HPO
4/ 0.01%CaSO
4, 37 ℃ of iron trichloride and the 2 molybdic acid hydrate sodium of trace, pH7.0～7.2, leavening temperatures, mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 48h; The porous-starch that in filtrating, adds filtrating weight 13%～16% respectively; 14～16% maltodextrin and 0.1% Povidone, USP/EP; After the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, be ring-type brown soccer star vinelandii CGMCC1.233 microbial inoculum.Colony counting method detects the bacterium number, gets Bacillus circulans 4.5～7.8 * 10
10CFU/g.
Bacterium powder 1,2 is mixed by 1: 1 (weight), be microbial inoculum B through vacuum packaging.
Straw mushroom bacterium slag special bacterium powder A and bacterium powder B are respectively applied for the zymophyte of straw mushroom bacterium slag and the auxiliary probiotic bacterium after the conduct fermentation; Use this composite fungus agent can obviously shorten the stack retting cycle of bacterium slag; Accelerating the macromolecular substance that xylogen, Mierocrystalline cellulose, semicellulose etc. are difficult to utilized by plant and animal in the stack retting process degrades; Thereby after making the fermentation of bacterium slag, its nutritive substance can be quicker, passive more completely plant utilization.Through the use of bacterium powder B, increased nitrogen-fixing microorganism in the bacterium slag, dissolve phosphorus, potassium microbial numbers, improved utilization ratio to inorganic nitrogen, phosphorus, potassium, reduce chemical fertilizer and use, practice thrift cost, the protection environment.
Embodiment
Below in conjunction with specific embodiment, the present invention is elaborated.
The microorganism used therefor material is known common used material among the present invention, and the public can buy from relevant preservation mechanism.
The invention provides little humification microbial inoculum of a kind of straw mushroom bacterium slag and preparation method thereof.This microbiobacterial agent comprises bacterium powder A, bacterium powder B, and its making method is:
Bacterium powder A: will cultivate 14～16h, cell concentration is 10
7～10
8The Pseudomonas stutzeri ACCC 01977,10 of CFU/mL
7～10
8The subtilis ACCC 11089,10 of CFU/mL
7～10
8The flat lead fungi ACCC30414 of the yellow spore of CFU/mL mixed in 1: 1: 1 by volume, after the mixing, inserted in the aseptic liquid nutrient medium by inoculum size 10% (V/V).Culture medium condition (w/v): 3% dregs of beans, 1.5% Semen Maydis powder saccharification liquid (v/v), 2% glucose, 0.3%KH
2PO
4, 0.15%MgSO
4, 37 ℃ of pH7.0, leavening temperature, mixing speed 120r/min, dissolved oxygen 20%; Bacterium liquid filters through 80 order strainers after cultivating 36h; In filtrating, add the porous-starch of filtrating weight 13%～16%; The maltodextrin and 0.1% Povidone, USP/EP of filtrating weight 14～16%; After spray-dried, add filtrating weight 4% superphosphate of lime and do weighting agent, the vacuum packaging of gained bacterium powder is bacterium powder A.Colony counting method detects the bacterium number, and Pseudomonas stutzeri ACCC01977, subtilis ACCC11089, flat lead fungi ACCC 30414 colony counts alive of yellow spore are respectively: 1.3～3.3 * 10
10CFU/g, 5.9～9..1 * 10
10CFU/g, 1.8～4.5 * 10
11CFU/g.
Bacterium powder B is made up of bacterium powder 1, bacterium powder 2.
Bacterium powder 1: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of bacillus cereus CGMCC 1.2223 bacterium liquid of CFU/mL is inoculated in the aseptic liquid nutrient medium; Culture medium condition (w/v): 1% Semen Maydis powder saccharification liquid (v/v); 37 ℃ of 1% peptones, 0.3% Carnis Bovis seu Bubali cream, 0.02%NaCl, pH7.0～7.2, leavening temperature; Mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 36h; The porous-starch that in filtrating, adds filtrating weight 13%～16% respectively; 14～16% maltodextrin and 0.1% Povidone, USP/EP; After the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, be bacillus cereus CGMCC 1.2223 microbial inoculums.Colony counting method detects the bacterium number, gets Bacillus circulans 3.7～6.9 * 10
10CFU/g.
Bacterium powder 2: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of aztobacter sp CGMCC1.233 bacterium liquid of CFU/mL is inoculated in the aseptic liquid nutrient medium culture medium condition (w/v): 0.05% yeast powder, 0.2% N.F,USP MANNITOL, 0.02%KH
2PO
4, 0.02%MgSO
4, 0.08%K
2HPO
4/ 0.01%CaSO
4, 37 ℃ of iron trichloride and the 2 molybdic acid hydrate sodium of trace, pH7.0～7.2, leavening temperatures, mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 48h; The porous-starch that in filtrating, adds filtrating weight 13%～16% respectively; 14～16% maltodextrin and 0.1% Povidone, USP/EP; After the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, be ring-type brown soccer star vinelandii CGMCC1.233 microbial inoculum.Colony counting method detects the bacterium number, gets Bacillus circulans 4.5～7.8 * 10
10CFU/g.
Bacterium powder 1, bacterium powder 2 are mixed by 1: 1 (weight), be microbial inoculum B through vacuum packaging.
During use, the fs, according to bacterium powder A: the mass ratio of bacterium slag=1: 500 inserts the bacterium slag with bacterium powder A, mixing, and moisturizing to humidity is 60%.Bacterium slag muck after will handling then is built into high 50cm, wide 70cm, and the trapezoidal heap body of long 120cm, and carry out artificial turning at 5d, 15d, 25d, 35d, the 45d of compost.And the lost situation of in the process of banking up, observing moisture every day, if moisture is low excessively, moisturizing immediately, the time of banking up is 45 days.
Subordinate phase, according to 1: 500 mixing of mass ratio, moisturizing to humidity is 60% with bacterium powder B and the bacterium slag behind the fs stack retting.Bacterium slag muck after will handling then is built into high 50cm, wide 70cm, and the trapezoidal heap body of long 120cm, and carry out artificial turning at 5d, 10d, the 15d of compost.And the lost situation of in the process of banking up, observing moisture every day, if moisture is low excessively, moisturizing immediately, the time of banking up is 15 days.
The full N content of organic fungi-manure behind the compost has increased by 71.30% than the compost initial stage, and full P content has increased by 125.53% than the compost initial stage, and full K content has increased by 57.37% than the compost initial stage.This bacterial manure is applied to cultivation Rose, flower of Greenish Lily, and all showing the leafing look dark green, and blade is thick and tall and straight, and plant strain growth is short strong; Every batch of amount of blooming of rose individual plant increases by 34.15%, longer blooming period 1.6d, and diseased plant rate reduces 6.28%; Every batch of amount of blooming of liliaceous individual plant increases by 31.03%, longer blooming period 2.2d, and diseased plant rate reduces 4.40%.Therefore, can be used as well flower culture fertilizer or direct as culture substrate.
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (2)
1. a straw mushroom bacterium slag humification complex bacterial agent special is characterized in that, comprises bacterium powder A, bacterium powder B; Bacterium powder A is used for the stack retting stage of straw mushroom bacterium slag, and bacterium powder B is as the straw mushroom bacterium slag of assisting after probiotic bacterium is added stack retting to; Bacterium powder A: will cultivate 14～16h, cell concentration is 10
7～10
8The Pseudomonas stutzeri of CFU//mL, subtilis, the flat lead fungi of yellow spore mixed in 1: 1: 1 by volume; After the mixing; Insert in the aseptic liquid nutrient medium culture medium condition (w/v): 3% dregs of beans, 1.5% Semen Maydis powder saccharification liquid (v/v), 2% glucose, 0.3%KH by inoculum size 10% (V/V)
2PO
4, 0.15%MgSO
4, 37 ℃ of pH7.0, leavening temperature, mixing speed 120r/min, dissolved oxygen 20%; Bacterium liquid filters through 80 order strainers after cultivating 36h; In filtrating, add the porous-starch of filtrating weight 13%～16%, the maltodextrin and 0.1% Povidone, USP/EP of filtrating weight 14～16%, spray-dried after; Add filtrating weight 4% superphosphate of lime and do weighting agent, be bacterium powder A;
Bacterium powder B is made up of bacterium powder 1, bacterium powder 2;
Bacterium powder 1: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of bacillus cereus bacterium liquid of CFU//mL is inoculated in the aseptic liquid nutrient medium; Culture medium condition (w/v): 1% Semen Maydis powder saccharification liquid (v/v); 37 ℃ of 1% peptones, 0.3% Carnis Bovis seu Bubali cream, 0.02%NaCl, pH7.0～7.2, leavening temperature; Mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 36h, in filtrating, add the porous-starch of filtrating weight 13%～16% respectively, 14～16% maltodextrin and 0.1% Povidone, USP/EP after the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, are bacterium powder 1;
Bacterium powder 2: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of aztobacter sp bacterium liquid of CFU//mL is inoculated in the aseptic liquid nutrient medium culture medium condition (w/v): 0.05% yeast powder, 0.2% N.F,USP MANNITOL, 0.02%KH
2PO
4, 0.02%MgSO
4, 0.0g%K
2HPO
4/ 0.01%CaSO
4, 37 ℃ of iron trichloride and the 2 molybdic acid hydrate sodium of trace, pH7.0～7.2, leavening temperatures, mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 48h, in filtrating, add the porous-starch of filtrating weight 13%～16% respectively, 14～16% maltodextrin and 0.1% Povidone, USP/EP after the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, are bacterium powder 2; The part by weight of bacterium powder 1,2 by 1: 1 mixed, be microbial inoculum B through vacuum packaging.
2. the making method of straw mushroom bacterium slag humification complex bacterial agent special is characterized in that, comprises bacterium powder A, bacterium powder B; Bacterium powder A is used for the stack retting stage of straw mushroom bacterium slag, and bacterium powder B is as the straw mushroom bacterium slag of assisting after probiotic bacterium is added stack retting to; Bacterium powder A: will cultivate 14～16h, cell concentration is 10
7～10
8The Pseudomonas stutzeri of CFU//mL, subtilis, the flat lead fungi of yellow spore mixed in 1: 1: 1 by volume; After the mixing; Insert in the aseptic liquid nutrient medium culture medium condition (w/v): 3% dregs of beans, 1.5% Semen Maydis powder saccharification liquid (v/v), 2% glucose, 0.3%KH by inoculum size 10% (V/V)
2PO
4, 0.15%MgSO
4, 37 ℃ of pH7.0, leavening temperature, mixing speed 120r/min, dissolved oxygen 20%; Bacterium liquid filters through 80 order strainers after cultivating 36h; In filtrating, add the porous-starch of filtrating weight 13%～16%, the maltodextrin and 0.1% Povidone, USP/EP of filtrating weight 14～16%, spray-dried after; Add filtrating weight 4% superphosphate of lime and do weighting agent, be bacterium powder A;
Bacterium powder B is made up of bacterium powder 1, bacterium powder 2;
Bacterium powder 1: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of bacillus cereus bacterium liquid of CFU//mL is inoculated in the aseptic liquid nutrient medium; Culture medium condition (w/v): 1% Semen Maydis powder saccharification liquid (v/v); 37 ℃ of 1% peptones, 0.3% Carnis Bovis seu Bubali cream, 0.02%NaCl, pH7.0～7.2, leavening temperature; Mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 36h, in filtrating, add the porous-starch of filtrating weight 13%～16% respectively, 14～16% maltodextrin and 0.1% Povidone, USP/EP after the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, are bacterium powder 1;
Bacterium powder 2: will cultivate 12～16h, cell concentration is 10
7～10
81: 10 by volume ratio of aztobacter sp bacterium liquid of CFU//mL is inoculated in the aseptic liquid nutrient medium culture medium condition (w/v): 0.05% yeast powder, 0.2% N.F,USP MANNITOL, 0.02%KH
2PO
4, 0.02%MgSO
4, 0.08%K
2HPO
4/ 0.01%CaSO
4, 37 ℃ of iron trichloride and the 2 molybdic acid hydrate sodium of trace, pH7.0～7.2, leavening temperatures, mixing speed 120r/min, dissolved oxygen 20%; Cross 80 order strainers after cultivating 48h, in filtrating, add the porous-starch of filtrating weight 13%～16% respectively, 14～16% maltodextrin and 0.1% Povidone, USP/EP after the spraying drying, add filtrating weight 4% superphosphate of lime and do weighting agent, are bacterium powder 2; The part by weight of bacterium powder 1,2 by 1: 1 mixed, be microbial inoculum B through vacuum packaging.
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| CN201110424714.1A CN102557767B (en) | 2011-12-19 | 2011-12-19 | Special compound fungus agent for humification of fungus residues of straw mushrooms and manufacturing method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105272523A (en) * | 2015-09-24 | 2016-01-27 | 姜淑琼 | Composite fertilizer for producing zero pesticide residue foods, and preparation method thereof |
| CN108018239A (en) * | 2017-12-25 | 2018-05-11 | 新疆农业科学院生物质能源研究所 | A kind of salt tolerant cellulose degradation microbial inoculum and preparation method and application |
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| CN101805697A (en) * | 2010-04-01 | 2010-08-18 | 江苏大学 | Vinegar residue fermented complex micro organism fungicide and preparation method thereof |
| CN102154108A (en) * | 2011-01-19 | 2011-08-17 | 山东省科学院能源研究所 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
| CN102174583A (en) * | 2011-02-22 | 2011-09-07 | 福州大学 | Method for producing biological humic acid by mixed fermentation |
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2011
- 2011-12-19 CN CN201110424714.1A patent/CN102557767B/en not_active Expired - Fee Related
Patent Citations (4)
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| CN1511940A (en) * | 2002-12-31 | 2004-07-14 | 福建农林大学 | Compound microbe fermentation strain preparing method and its use |
| CN101805697A (en) * | 2010-04-01 | 2010-08-18 | 江苏大学 | Vinegar residue fermented complex micro organism fungicide and preparation method thereof |
| CN102154108A (en) * | 2011-01-19 | 2011-08-17 | 山东省科学院能源研究所 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
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| CN105272523A (en) * | 2015-09-24 | 2016-01-27 | 姜淑琼 | Composite fertilizer for producing zero pesticide residue foods, and preparation method thereof |
| CN108018239A (en) * | 2017-12-25 | 2018-05-11 | 新疆农业科学院生物质能源研究所 | A kind of salt tolerant cellulose degradation microbial inoculum and preparation method and application |
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