CN102550756B - Compound health-care tea containing vine tea and preparation method thereof - Google Patents
Compound health-care tea containing vine tea and preparation method thereof Download PDFInfo
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Abstract
The invention provides compound health-care tea containing vine tea. The compound health-care tea comprises the following raw materials in part by weight: 80 to 200 parts of tender leaf of the vine tea, 30 to 60 parts of tender stem and branch of the vine tea, 30 to 60 parts of vine tea root, 5 to 20 parts of mulberry leaf, 5 to 20 parts of lotus leaf and 1 to 10 parts of chrysanthemum. The compound health-care tea has obvious effects of reducing blood fat and blood pressure, has the faint scent of the lotus leaves, the chrysanthemum and the like and is fragrant and sweet in taste. Simultaneously, a preparation process for the compound health-care tea is simple, and is suitable for large-scale production. In addition, the compound health-care tea is prepared from simple raw materials and low in cost, has the good effects of reducing blood fat and blood pressure, and is a health-care tea with high practicability.
Description
Technical field
Herbal health care field of food of the present invention specifically refers to a kind of vine tea compound health-care tea and preparation method thereof.
Background technology
Vine tea, Chinese plant is called ampelopsis grossdentata, is commonly called as Maoyanmei tea, Dragon Boat Festival tea, rattan mother-in-law tea, is a kind of Wild Liane that belongs to the Vitaceae Ampelopsis.This dark brown green bloom; The sweet length of little hardship promotes the production of body fluid to quench thirst; Its flavor is sweet cool in nature, has clearing heat and detoxicating, anti-inflammation, dispels rheumatism, the effects such as strengthening the bones and muscles, hypotensive, reducing blood lipid, liver protecting.The control that is usually used in the diseases such as high blood pressure, cold, fever, cardiovascular and cerebrovascular disease, dermatitis, eczema among the people.Contain a large amount of flavone compounds in the vine tea, its main substance is dibydro myricetrin, and it up to 73.3-91.5%, can alleviate oxidative damage in the body to the clearance rate of free radical, has antidotal effect.Vine tea can also alleviate sex change and the degree of necrosis of animal liver tissue, and the effect of liver protecting is arranged.The report amur ampelopsis roots (being the vine tea root) such as Lingling Yang in 1987 and mutation Ampelopsis root thereof can significantly reduce the glutamic-pyruvic transaminase that raises due to carbon tetrachloride and the galactosamine.According to another the composition Cg ester that has the anti-hepatic fibrosis activity in the Japanese patent laid-open publication gazette amur ampelopsis root (being the vine tea root), and patent applied for.Therefore vine tea is a kind of drink tea with very many effects, but at present vine tea is used for the still rare of blood fat-reducing blood pressure-decreasing as health protection tea, and blood fat-reducing blood pressure-decreasing effect and not obvious.
Summary of the invention
Main purpose of the present invention provides a kind of compound health-care tea that contains vine tea, makes its effect with blood fat-reducing blood pressure-decreasing, and comfortable taste.
The present invention is achieved through the following technical solutions: a kind of compound health-care tea that contains vine tea, the parts by weight of its raw material are: vine tea tender leaf 80-200 part, the tender stem branch of vine tea 30-60 part, vine tea root 30-60 part, mulberry leaf 5-20 part, lotus leaf 5-20 part, chrysanthemum 1-10 part.
Preferably, 150 parts of vine tea tender leafs, 40 parts in the tender stem branch of vine tea, 50 parts of vine tea roots, 10 parts on mulberry leaf, 10 parts on lotus leaf, 5 parts of chrysanthemums.
Another object of the present invention provides a kind of preparation method who contains the compound health-care tea of vine tea.
For realizing this purpose, the present invention adopts following technical scheme: a kind ofly prepare the above-mentioned method that contains the compound health-care tea of vine tea, it comprises the steps: that (1) is that the vine tea tender leaf of 80-200 part is cleaned and to be dried rear frying and complete after the cooling with parts by weight, spray concentration is that 80% ethanol is spread the vine tea tender leaf after obtaining processing after drying in the air 20-30 processed hour thereon afterwards out, and described ethanol weight is the 8-12% of rear tender leaf weight of completing; (2) with parts by weight be the vine tea root concentration of the tender stem branch of vine tea of 30-60 part and 30-60 part be 60-80% alcohol extract and concentrated after obtain concentrate; (3) chrysanthemum that the vine tea tender leaf after will processing and parts by weight are the mulberry leaf of 5-20 part, lotus leaf that parts by weight are 5-20 part, parts by weight are 1-10 part mix and pulverize after obtain corase meal, above-mentioned concentrate is sprayed on described corase meal, being granulation into length through extruding is the 2-4mm particle, again particle is carried out drying, dried particles through the sterilization after, through can, the packing and get final product.
Preferably, frying referred in 100-120 ℃ iron pan frying 20 minutes in the step (1).
Preferably, extracting in the step (2) is to be that the ethanol of 60-80% is heated to 40 ℃ of extractions 2 times with concentration, extracts 2 hours at every turn, and the consumption of ethanol is the tender stem branch of vine tea and vine tea root weight 8 times when extracting at every turn.
Preferably, concentrated in the step (2) is that reduced pressure concentration becomes concentrate, and described concentrate is 1.05-1.10 60 ℃ of lower relative densities of surveying.
Preferably, first the tender stem branch of vine tea and vine tea undercut are made length at the fritter of 0.5-1.0cm in the step (2).
Preferably, the described corase meal of step (3) is 10 orders, and described drying is that to be dried to moisture under 80 ℃ be below 10%, and described sterilization is through 6-8kGy dosage 60Coradiation.
Optimum technical scheme is: a kind ofly prepare the above-mentioned method that contains the compound health-care tea of vine tea, it comprise the steps: (1) with parts by weight be 150 parts vine tea tender leaf clean dry after in 100-120 ℃ iron pan frying completed in 20 minutes after the cooling, spray concentration is that 80% ethanol is spread the vine tea tender leaf after obtaining processing after drying in the air 20-30 processed hour thereon afterwards out, and described ethanol weight is the 8-12% of rear tender leaf weight of completing; (2) be that 40 parts the tender stem branch of vine tea and 50 parts vine tea undercut are made length at the fritter of 0.5-1.0cm with parts by weight, be that the ethanol of 60-80% is heated to 40 ℃ and extracts 2 times with concentration, the each extraction 2 hours, the consumption of ethanol is the tender stem branch of vine tea and vine tea root weight 8 times during each the extraction, and reduced pressure concentration becomes concentrate, and described concentrate is 1.05-1.10 60 ℃ of lower relative densities of surveying; (3) chrysanthemum that the vine tea tender leaf after will processing and parts by weight are 10 parts mulberry leaf, lotus leaf that parts by weight are 10 parts, parts by weight are 5 parts mix and pulverize after obtain 10 purpose corase meals, above-mentioned concentrate is sprayed on described corase meal, being granulation into length through extruding is the 2-4mm particle, again particle being dried to moisture under 80 ℃ is below 10%, dried particles is after 6-8kGy dosage 60Coradiation sterilization, through can, packing and get final product.
Contain the flavones ingredient of strengthening capillary, reducing blood viscosity in the mulberry leaf, Mulberry-leaf Tea contains the composition of LDL-lipoprotein oxidation in the antibody in addition, so mulberry leaf have again the effect of prevention myocardial infarction and cerebral hemorrhage when fat-reducing, improving hyperlipidemia.Studies confirm that the hyperlipidemia serum lipids is raise mulberry-leaf extract and atherosclerotic has inhibitory action.Mulberry-leaf Tea can make hyperlipidemia rats serum high-density LP (HDL-C), HDL-C/TC obviously raise (p<0.05), T-CHOL (TC), LDL-C, triacylglycerol (TG) obviously reduce (p<0.05), lipid peroxide (LPO) significantly reduces (p<0.001), illustrate that Mulberry-leaf Tea can reduce blood fat, softening blood vessel, removing body endoperoxide, thereby the rising of hyperlipidemia serum lipids and atherosclerotic are had inhibitory action.
Chrysanthemum is coronary artery dilator obviously, and increases CBF, can strengthen capillary resistance.The chrysanthemum glucoside has hypotensive effect.
And the expansible blood vessel of the alkaloid in the lotus leaf, clearing away summerheat has hypotensive and effect reducing blood lipid.
The compound health-care tea that contains vine tea of the present invention has the effect of significant blood fat-reducing blood pressure-decreasing, and contains the delicate fragrance such as lotus leaf chrysanthemum in this compound health-care tea, so taste is fragrant and sweet.The preparation technology of compound health-care tea of the present invention is simple simultaneously, is fit to large-scale production.In addition, the raw material that the present invention adopts is simple, with low cost, and blood fat-reducing blood pressure-decreasing is effective, is a kind of health protection tea that is very practical.
Description of drawings
Fig. 1 is the Blood Pressure Experiment experimental group result of health protection tea of the present invention;
Fig. 2 is the Blood Pressure Experiment control group result of health protection tea of the present invention;
The specific embodiment
Further specify the present invention below in conjunction with specific embodiment:
Embodiment 1:
Be that 200 parts vine tea tender leaf is got express developed totally with purified water with parts by weight, put shady and cool place and dry, then frying 20 minutes in 120 ℃ of iron pans, let cool rear usefulness be equivalent to complete rear tender leaf weight 12% 80% ethanol spray in the above, spread out and hang 30h.
Be the fritter that 60 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 60 parts are grown into 0.5-1.0cm with parts by weight, being heated to 40 ℃ with 8 times of amount 60% ethanol extracts 2 times, the each extraction 2 hours, it is the concentrate of 1.10 (60 ℃ of surveys) that the extract reduced pressure concentration becomes relative density;
The mulberry leaf that the vine tea tender leaf processed and parts by weight are 20 parts, the lotus leaf that parts by weight are 20 parts, the Hang Ju that parts by weight are 10 parts mix, be ground into 10 order corase meals, then above-mentioned concentrate is evenly sprayed in corase meal, stir, being granulation into length through extruding is the 2-4mm particle, again with particle vacuum drying under 80 ℃ of conditions, the dried pellet moisture of surveying is 10%, then with dried particle through the 60Coradiation of 6-8kGy dosage sterilize up to standard to microbiological indicator after, through can fill, packing namely gets the compound health-care tea 1 that contains vine tea.
Embodiment 2:
Be that 80 parts vine tea tender leaf is got express developed totally with purified water with parts by weight, put shady and cool place and dry, then frying 20 minutes in 100 ℃ of iron pans, let cool rear usefulness be equivalent to complete rear tender leaf weight 8% 80% ethanol spray in the above, spread out and hang 20h.
Be the fritter that 30 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 30 parts are grown into 0.5-1.0cm with parts by weight, being heated to 40 ℃ with 8 times of amount 80% ethanol extracts 2 times, the each extraction 2 hours, it is the concentrate of 1.05 (60 ℃ of surveys) that the extract reduced pressure concentration becomes relative density;
The mulberry leaf that the vine tea tender leaf processed and parts by weight are 5 parts, the lotus leaf that parts by weight are 5 parts, the Hang Ju that parts by weight are 1 part mix, be ground into 10 order corase meals, then above-mentioned concentrate is evenly sprayed in corase meal, stir, being granulation into length through extruding is the 2-4mm particle, again with particle vacuum drying under 80 ℃ of conditions, the dried pellet moisture of surveying is 10%, then with dried particle through the 60Coradiation of 6-8kGy dosage sterilize up to standard to microbiological indicator after, through can fill, packing namely gets the compound health-care tea 2 that contains vine tea.
Embodiment 3:
Be that 150 parts vine tea tender leaf is got express developed totally with purified water with parts by weight, put shady and cool place and dry, then frying 20 minutes in 10 ℃ of iron pans, let cool rear usefulness be equivalent to complete rear tender leaf weight 10% 80% ethanol spray in the above, spread out and hang 24h.
Be the fritter that 40 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 50 parts are grown into 0.5-1.0cm with parts by weight, being heated to 40 ℃ with 8 times of amount 70% ethanol extracts 2 times, the each extraction 2 hours, it is the concentrate of 1.08 (60 ℃ of surveys) that the extract reduced pressure concentration becomes relative density;
The mulberry leaf that the vine tea tender leaf processed and parts by weight are 10 parts, the lotus leaf that parts by weight are 10 parts, the Hang Ju that parts by weight are 5 parts mix, be ground into 10 order corase meals, then above-mentioned concentrate is evenly sprayed in corase meal, stir, being granulation into length through extruding is the 2-4mm particle, again with particle vacuum drying under 80 ℃ of conditions, the dried pellet moisture of surveying is 10%, then with dried particle through the 60Coradiation of 6-8kGy dosage sterilize up to standard to microbiological indicator after, through can fill, packing namely gets the compound health-care tea 3 that contains vine tea.
Experimental example 1:
1 material
1.1 medicine
Get embodiment 3 (or embodiment 1,2) the gained compound health-care tea bag particle of making tea; Fenofibrate, 100mg/ grain, Aidifa Pharmaceutical Co., Ltd., Shanghai, lot number 110320; During experiment, respectively it is made into the suspension of desired concn for rat oral gavage with 0.5%CMC-Na.Propylthiouracil, the 50mg/ sheet. Shenzhen China Associated Pharmaceutical Co., Ltd. produces. lot number 101209.
1.2 reagent
Triglyceride (TG, 20100603), cholesterol (TC, 20100918), low-density lipoprotein (LDL-C, 20100601). high/low density lipoprotein (HDL-C, 20100723) measure kit, Shanghai Kehua Bio-engineering Co., Ltd produces; Cholesterol (20101103) and natrii tauroglycocholas (20110201) biochemical reagents are produced by extensive and profound in meaning star biochemical technology Co., Ltd of Shanghai biochemical technology Co., Ltd and Beijing respectively.
1.3 animal
The complete male SD rat of SPF level, body weight 180-220g is available from Disease Prevention Control Center, Hubei Prov.
1.4 instrument
The 21VtS-1024 automatic clinical chemistry analyzer, Tokyo Co., Ltd.;
AL104 type electronic analytical balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd.;
JJ2000 type electronic balance.Changshu City two outstanding tester factory;
The Centrifuge5810-R table-type high-speed refrigerated centrifuge, German eppendorf company;
1500 ELIASAs, Finland Lei Bo company.
1.5 statistics
The result with
Expression, the normal distribution data is checked with t, and skewness distributes and uses rank test.
2 methods
2.1 grouping and model copy
70 of male SD rats are after laboratory adaptability raised for 1 week. and get hematometry serum TC and TG, be divided at random 7 groups according to blood lipid level and body weight: Normal group; Model group; The fenofibrate group; Vine tea tea in bag experimental group; The basic, normal, high dosage group of vine tea tea in bag; Every group 10.Except Normal group was fed normal diet, all the other each treated animals were all fed high lipid food, freely ingested and drank water.During the moulding each treated animal morning every day on an empty stomach according to dosage regularly volume of gastric infusion be 10ml/kg, normal group and model group gavage isometric(al) 0.5%CMC-Na, successive administration 42d.
2.2 index detects
Respectively experiment 0,14,28,42d takes by weighing rat body weight, gets hematometry TC and TG from intraocular corner of the eyes rear vein beard.Water 12h is can't help in fasting after the last administration, uses the yellow Jackets anesthetized animal, and arteria carotis is got blood, and the 3000rpm separation of serum is pressed kit method and measured serum TG, TC, LDL-C, HDL-C
2.3 high lipid food prescription
1% cholesterol, 10% lard, 0.5% sodium taurocholate, 5% sucrose, 0.2% propylthiouracil, 83.3% basal feed.Behind hot lard dissolving cholesterol, mixing adds water and stirs, and the kneading frustillatum is inserted in the 80-100 ℃ of baking oven and dried.
3 results
3.1 on the impact of hyperlipidemia rats serum TG value, see the following form 1:
Table 1 is on the impact of hyperlipidemia rats serum TG value
Compare with blank:
△P<005,
△ △P<0.01; Compare with model group:
*P<0.05,
*P<0.01
By as seen from Table 1, rat fed high lipid food to the 14 days, and model group TG is significantly higher than the blank group, and maintains more stable level, show high TG model success: vine tea compound three dosage groups namely had in various degree reducing effect to serum TG on the 14th day from administration, and are more obvious with administration effect in 42 days.
3.2 on the impact of hyperlipidemia rats serum TC, see the following form 2:
Table 2 is on the impact of hyperlipidemia rats serum TG value
Compare with blank:
△P<0.05,
△ △P<0.01; Compare with model group:
*P<0.05,
*P<0.01
By as seen from Table 2,14 days model group TC of moulding obviously increase, and all maintain metastable level to 42 days, show high TC mass formed by blood stasis moulding success; The three dosage group administrations of vine tea compound can reduce rat model serum TC level in 28 days in various degree, and are more obvious to effect in 42 days.
3.3 on the impact of hyperlipidemia rats serum LDL-C and HDL-C, see the following form 3:
Table 3 is on the impact of hyperlipidemia rats serum LDL-C and HDL-C value
Compare with blank:
△P<0.05,
△ △P<0.01; Compare with model group:
*P<0.05,
*P<0.01
By as seen from Table 3,28 days model group LDL-C of moulding obviously raise, and still maintained more stable level to 42 days, of the present inventionly contained the LDL-C that vine tea compound health-care tea experimental group can reduce serum in various degree, and are best with administration effect in 42 days.
Experimental example 2:
1) sample preparation
Get embodiment 3 (or embodiment 1,2) gained tea in bag particle 50g, boiling was filtered after 40 minutes, got filtrate, and adding physiological saline to amount of solution is 100ml, namely is to make every ml liquid be equivalent to the 0.5g particle, for subsequent use; The 50mg Captopril tablets is pulverized again, add 100ml physiological saline heating for dissolving, get supernatant after leaving standstill and make injection, namely injection concentration is 0.5mg/ml.
2) experimental technique
Take rabbit as experimental subjects, totally 4, body weight is about 2kg.Be equally divided into 2 groups, one group is experimental group, and one group is control group.Rabbit is cooked the arteria carotis intubate, connects pressure transducer to physiograph, and recording blood pressure changes.
Experimental group is observed the rabbit blood pressure change with the extract auricular vein injection of 1ml/kg.Until the steadily rear quiet notes captopril 0.25mg/kg of blood pressure, observe blood pressure and have or not again significant change.
Control group is observed the rabbit blood pressure change with the injection of captopril 0.25mg/kg auricular vein.The extract of quiet notes 1ml/kg after blood pressure is steady is observed blood pressure and is had or not again significant change.
3) experimental result
Experimental group is as shown in Figure 1: after the experimental group step-down, blood pressure gos up gradually, but the rise amplitude is less, reaches steady after about 10 minutes.But blood pressure can not return to the front level of injection.
Until the steadily rear quiet notes captopril 0.25mg/kg of experimental group blood pressure, blood pressure is without again significant change.
Control group is as shown in Figure 2: after the control group step-down, the very fast appearance of blood pressure is gone up, but the rise amplitude is little, reaches steady after about 4 minutes.Blood pressure recovers near level before the injection.
The extract of quiet notes 1ml/kg after the control group blood pressure is steady, blood pressure has more obviously decline.
4) conclusion
The compound health-care tea that contains vine tea of the present invention has the effect of reduction of blood pressure in high-speed, and can keep preferably low pressure level.
Claims (8)
1. compound health-care tea that contains vine tea, it is characterized in that: the parts by weight of its raw material are: vine tea tender leaf 80-200 part, the tender stem branch of vine tea 30-60 part, vine tea root 30-60 part, mulberry leaf 5-20 part, lotus leaf 5-20 part, chrysanthemum 1-10 part.
2. the described compound health-care tea that contains vine tea according to claim 1, it is characterized in that: the parts by weight of its raw material are: 150 parts of vine tea tender leafs, 40 parts in the tender stem branch of vine tea, 50 parts of vine tea roots, 10 parts on mulberry leaf, 10 parts on lotus leaf, 5 parts of chrysanthemums.
3. one kind prepares the described method that contains the compound health-care tea of vine tea of claim 1, it is characterized in that: it comprises the steps: that ⑴ is that the vine tea tender leaf of 80-200 part is cleaned and to be dried rear frying and complete after the cooling with parts by weight, spray concentration is that 80% ethanol is spread the vine tea tender leaf after obtaining processing after drying in the air 20-30 processed hour thereon afterwards out, and described ethanol weight is the 8-12% of rear tender leaf weight of completing; With parts by weight be the vine tea root concentration of the tender stem branch of vine tea of 30-60 part and 30-60 part be 60-80% alcohol extract and concentrated after obtain concentrate; ⑶ the chrysanthemum that the vine tea tender leaf after will process and parts by weight are the mulberry leaf of 5-20 part, lotus leaf that parts by weight are 5-20 part, parts by weight are 1-10 part mix and pulverize after obtain corase meal, above-mentioned concentrate is sprayed on described corase meal, being granulation into length through extruding is the 2-4mm particle, again particle is carried out drying, dried particles through the sterilization after, through can, the packing and get final product.
4. the described method that contains the compound health-care tea of vine tea according to claim 3 is characterized in that: frying referred in 100-120 ℃ iron pan frying 20 minutes among the step ⑴.
5. the described method that contains the compound health-care tea of vine tea according to claim 3, it is characterized in that: extracting among the step ⑵ is to be that the ethanol of 60-80% is heated to 40 ℃ of extractions 2 times with concentration, the each extraction 2 hours, the consumption of ethanol is the tender stem branch of vine tea and vine tea root weight 8 times when extracting at every turn.
6. the described method that contains the compound health-care tea of vine tea according to claim 3, it is characterized in that: concentrated among the step ⑵ is that reduced pressure concentration becomes concentrate, described concentrate is 1.05-1.10 60 ℃ of lower relative densities of surveying.
7. the described method that contains the compound health-care tea of vine tea according to claim 3 is characterized in that: first the tender stem branch of vine tea and vine tea undercut are made length at the fritter of 0.5-1.0cm among the step ⑵.
8. the described method that contains the compound health-care tea of vine tea according to claim 3, it is characterized in that: the described corase meal of step ⑶ is 10 orders, and described drying is that to be dried to moisture under 80 ℃ be below 10%, and described sterilization is through 6-8kGy dosage 60Coradiation.
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CN100518533C (en) * | 2007-02-14 | 2009-07-29 | 湖南宏兴隆湘莲食品有限公司 | Lotus-leaf lotus-plumule herb tea |
CN101642179B (en) * | 2009-09-08 | 2011-12-07 | 项金秀 | Tea beverage with functions of lowering pressure, lowering sugar and lowering fat |
CN102160588B (en) * | 2010-12-17 | 2012-11-21 | 张家界灵洁绿色食品有限公司 | Functional tea and preparation method thereof |
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