CN102550756A - Compound health-care tea containing vine tea and preparation method thereof - Google Patents
Compound health-care tea containing vine tea and preparation method thereof Download PDFInfo
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Abstract
The invention provides compound health-care tea containing vine tea. The compound health-care tea comprises the following raw materials in part by weight: 80 to 200 parts of tender leaf of the vine tea, 30 to 60 parts of tender stem and branch of the vine tea, 30 to 60 parts of vine tea root, 5 to 20 parts of mulberry leaf, 5 to 20 parts of lotus leaf and 1 to 10 parts of chrysanthemum. The compound health-care tea has obvious effects of reducing blood fat and blood pressure, has the faint scent of the lotus leaves, the chrysanthemum and the like and is fragrant and sweet in taste. Simultaneously, a preparation process for the compound health-care tea is simple, and is suitable for large-scale production. In addition, the compound health-care tea is prepared from simple raw materials and low in cost, has the good effects of reducing blood fat and blood pressure, and is a health-care tea with high practicability.
Description
Technical field
Herbal health care field of food of the present invention specifically is meant a kind of vine tea compound health-care tea and preparation method thereof.
Background technology
Vine tea, Chinese plant is called ampelopsis grossdentata, is commonly called as Maoyanmei tea, Dragon Boat Festival tea, rattan mother-in-law tea, is a kind of wild liana that belongs to the Vitaceae Ampelopsis.This dark brown green bloom; The sweet length of little hardship promotes the production of body fluid to quench thirst; Its flavor is sweet cool in nature, has clearing heat and detoxicating, anti-inflammation, dispels rheumatism, effects such as strengthening the bones and muscles, hypotensive, reducing blood lipid, liver protecting.The control that is usually used in diseases such as high blood pressure, cold, fever, cardiovascular and cerebrovascular disease, dermatitis, eczema among the people.Contain a large amount of flavone compounds in the vine tea, its main substance is a dibydro myricetrin, and it up to 73.3-91.5%, can alleviate oxidative damage in the body to the clearance rate of free radical, has antidotal effect.Vine tea can also alleviate the sex change and the degree of necrosis of animal liver tissue, and the effect of liver protecting is arranged.Report amur ampelopsis roots (being the vine tea root) such as Lingling Yang in 1987 and mutation Ampelopsis root thereof can significantly reduce the glutamic-pyruvic transaminase that raises due to carbon tetrachloride and the galactosamine.According to another the composition gallic acid catechin ester that has the anti-hepatic fibrosis activity in the japanese patent laid-open publication gazette amur ampelopsis root (being the vine tea root), and patent applied for.Therefore vine tea is a kind of drink tea with very many effects, but at present vine tea is used for the still rare of blood fat-reducing blood pressure-decreasing as health protection tea, and blood fat-reducing blood pressure-decreasing effect and not obvious.
Summary of the invention
Main purpose of the present invention provides a kind of compound health-care tea that contains vine tea, makes its effect with blood fat-reducing blood pressure-decreasing, and comfortable taste.
The present invention realizes through following technical scheme: a kind of compound health-care tea that contains vine tea; Its raw materials in part by weight is: vine tea tender leaf 80-200 part; The tender stem branch of vine tea 30-60 part, vine tea root 30-60 part, mulberry leaf 5-20 part, lotus leaf 5-20 part, chrysanthemum 1-10 part.
Preferably, 150 parts of vine tea tender leafs, 40 parts in the tender stem branch of vine tea, 50 parts of vine tea roots, 10 parts on mulberry leaf, 10 parts on lotus leaf, 5 parts of chrysanthemums.
Another object of the present invention provides a kind of preparation method who contains the compound health-care tea of vine tea.
For realizing this purpose; The present invention adopts following technical scheme: a kind ofly prepare the above-mentioned method that contains the compound health-care tea of vine tea; It comprises the steps: that (1) is that the vine tea tender leaf of 80-200 part is cleaned and to be dried the back frying and complete after the cooling with parts by weight; Spray concentration is that 80% ethanol is spread the vine tea tender leaf after the system of drying in the air obtained handling after 20-30 hour out after on it, and said ethanol weight is the 8-12% of back tender leaf weight of completing; (2) be that the vine tea root of the tender stem branch of vine tea and 30-60 part of 30-60 part uses concentration to obtain concentrate as the alcohol extract of 60-80% and after concentrating with parts by weight; (3) chrysanthemum that the vine tea tender leaf after will handling and parts by weight are the mulberry leaf of 5-20 part, lotus leaf that parts by weight are 5-20 part, parts by weight are 1-10 part mix and pulverize after obtain corase meal; Above-mentioned concentrate is sprayed on said corase meal; Granulating into length through extruding is the 2-4mm particle; Again particle is carried out drying, dried particles promptly gets through can, packing after sterilization.
Preferably, frying was meant in 100-120 ℃ iron pan frying 20 minutes in the step (1).
Preferably, extracting in the step (2) is to use concentration to be heated to 40 ℃ of extractions 2 times as the ethanol of 60-80%, extracts 2 hours at every turn, and consumption of ethanol is tender stem branch of vine tea and vine tea root weight 8 times when extracting at every turn.
Preferably, concentrating in the step (2) is that decompression is condensed into concentrate, and said concentrate is 1.05-1.10 60 ℃ of relative densities of surveying down.
Preferably, earlier tender stem branch of vine tea and vine tea undercut are processed the fritter of length at 0.5-1.0cm in the step (2).
Preferably, the said corase meal of step (3) is 10 orders, and said drying is that under 80 ℃, to be dried to moisture be below 10%, and said sterilization is through 6-8kGy dosage cobalt 60 irradiation.
Optimum technical scheme is: a kind ofly prepare the above-mentioned method that contains the compound health-care tea of vine tea; It comprises the steps: that (1) is that 150 parts vine tea tender leaf is cleaned and to be dried back frying in 100-120 ℃ iron pan and completed in 20 minutes after the cooling with parts by weight; Spray concentration is that 80% ethanol is spread the vine tea tender leaf after the system of drying in the air obtained handling after 20-30 hour out after on it, and said ethanol weight is the 8-12% of back tender leaf weight of completing; (2) be that 40 parts tender stem branch of vine tea and 50 parts vine tea undercut are processed the fritter of length at 0.5-1.0cm with parts by weight; Use concentration to be heated to 40 ℃ of extractions 2 times as the ethanol of 60-80%; The each extraction 2 hours; Consumption of ethanol is the tender stem branch of vine tea and vine tea root weight 8 times during each the extraction, and decompression is condensed into concentrate, said concentrate 60 ℃ down the relative densities of survey be 1.05-1.10; (3) chrysanthemum that the vine tea tender leaf after will handling and parts by weight are 10 parts mulberry leaf, lotus leaf that parts by weight are 10 parts, parts by weight are 5 parts mix and pulverize after obtain 10 purpose corase meals; Above-mentioned concentrate is sprayed on said corase meal; Granulating into length through extruding is the 2-4mm particle; Again particle being dried to moisture under 80 ℃ is below 10%, and dried particles promptly gets through can, packing behind 6-8kGy dosage cobalt 60 irradiation sterilizations.
Contain the flavones ingredient of strengthening capillary, reducing blood viscosity in the mulberry leaf, Mulberry-leaf Tea contains the composition of LDL-lipoprotein oxidation in the antibody in addition, so mulberry leaf have the effect of prevention myocardial infarction and cerebral hemorrhage again when fat-reducing, improving hyperlipidemia.Have research to confirm, the hyperlipidemia serum lipids is raise mulberry-leaf extract and atherosclerotic has inhibitory action.Mulberry-leaf Tea can make hyperlipidemia rats serum high-density LP (HDL-C), HDL-C/TC obviously raise (p＜0.05); T-CHOL (TC), LDL-C, triacylglycerol (TG) obviously reduce (p＜0.05); Lipid peroxide (LPO) significantly reduces (p＜0.001); Explain that Mulberry-leaf Tea can reduce blood fat, softening blood vessel, removing body endoperoxide, thereby rising of hyperlipidemia serum lipids and atherosclerotic are had inhibitory action.
Chrysanthemum is coronary artery dilator obviously, and increases CBF, can strengthen capillary resistance.The chrysanthemum glucoside has hypotensive effect.
And the expansible blood vessel of the alkaloid in the lotus leaf, clearing away summerheat has hypotensive and effect reducing blood lipid.
The compound health-care tea set that contains vine tea of the present invention has the effect of significant blood fat-reducing blood pressure-decreasing, and contains delicate fragrance such as lotus leaf chrysanthemum in this compound health-care tea, so taste is fragrant and sweet.The preparation technology of compound health-care tea of the present invention is simple simultaneously, is fit to large-scale production.In addition, the raw material that the present invention adopts is simple, with low cost, and blood fat-reducing blood pressure-decreasing is effective, is a kind of health protection tea that is very practical.
Description of drawings
Fig. 1 is the blood pressure experiments experiment group result of health protection tea of the present invention;
Fig. 2 is the blood pressure experiment contrast group result of health protection tea of the present invention;
The specific embodiment
Further specify the present invention below in conjunction with specific embodiment:
Embodiment 1:
With parts by weight is that 200 parts vine tea tender leaf is got express developed totally with purified water, puts shady and cool place and dries, and frying 20 minutes in 120 ℃ of iron pans is then put cold back with the 80% ethanol spray of the back tender leaf weight 12% that is equivalent to complete in the above, spreads out and hangs 30h.
With parts by weight is the fritter that 60 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 60 parts are grown into 0.5-1.0cm; Being heated to 40 ℃ with 8 times of amount 60% ethanol extracts 2 times; The each extraction 2 hours, the extract decompression is condensed into the concentrate that relative density is 1.10 (60 ℃ of surveys);
With the vine tea tender leaf of handling and parts by weight is that 20 parts mulberry leaf, the lotus leaf that parts by weight are 20 parts, the Hang Ju that parts by weight are 10 parts mix; Be ground into 10 order corase meals; Then above-mentioned concentrate is evenly sprayed in corase meal, stir, granulating into length through extruding is the 2-4mm particle; Again with particle vacuum drying under 80 ℃ of conditions; The dried pellet moisture of surveying is 10%, then with dried particle after cobalt 60 irradiation sterilizations of 6-8kGy dosage are up to standard to microbiological indicator, through can fill, packing promptly gets the compound health-care tea 1 that contains vine tea.
Embodiment 2:
With parts by weight is that 80 parts vine tea tender leaf is got express developed totally with purified water, puts shady and cool place and dries, and frying 20 minutes in 100 ℃ of iron pans is then put cold back with the 80% ethanol spray of the back tender leaf weight 8% that is equivalent to complete in the above, spreads out and hangs 20h.
With parts by weight is the fritter that 30 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 30 parts are grown into 0.5-1.0cm; Being heated to 40 ℃ with 8 times of amount 80% ethanol extracts 2 times; The each extraction 2 hours, the extract decompression is condensed into the concentrate that relative density is 1.05 (60 ℃ of surveys);
With the vine tea tender leaf of handling and parts by weight is that 5 parts mulberry leaf, the lotus leaf that parts by weight are 5 parts, the Hang Ju that parts by weight are 1 part mix; Be ground into 10 order corase meals; Then above-mentioned concentrate is evenly sprayed in corase meal, stir, granulating into length through extruding is the 2-4mm particle; Again with particle vacuum drying under 80 ℃ of conditions; The dried pellet moisture of surveying is 10%, then with dried particle after cobalt 60 irradiation sterilizations of 6-8kGy dosage are up to standard to microbiological indicator, through can fill, packing promptly gets the compound health-care tea 2 that contains vine tea.
Embodiment 3:
With parts by weight is that 150 parts vine tea tender leaf is got express developed totally with purified water, puts shady and cool place and dries, and frying 20 minutes in 10 ℃ of iron pans is then put cold back with the 80% ethanol spray of the back tender leaf weight 10% that is equivalent to complete in the above, spreads out and hangs 24h.
With parts by weight is the fritter that 40 parts the tender stem branch of vine tea, vine tea undercut system that parts by weight are 50 parts are grown into 0.5-1.0cm; Being heated to 40 ℃ with 8 times of amount 70% ethanol extracts 2 times; The each extraction 2 hours, the extract decompression is condensed into the concentrate that relative density is 1.08 (60 ℃ of surveys);
With the vine tea tender leaf of handling and parts by weight is that 10 parts mulberry leaf, the lotus leaf that parts by weight are 10 parts, the Hang Ju that parts by weight are 5 parts mix; Be ground into 10 order corase meals; Then above-mentioned concentrate is evenly sprayed in corase meal, stir, granulating into length through extruding is the 2-4mm particle; Again with particle vacuum drying under 80 ℃ of conditions; The dried pellet moisture of surveying is 10%, then with dried particle after cobalt 60 irradiation sterilizations of 6-8kGy dosage are up to standard to microbiological indicator, through can fill, packing promptly gets the compound health-care tea 3 that contains vine tea.
Experimental example 1:
1 material
1.1 medicine
Get embodiment 3 (or embodiment 1,2) the gained compound health-care tea bag particle of making tea; Fenofibrate, 100mg/ grain, Aidifa Pharmaceutical Co., Ltd., Shanghai, lot number 110320; During experiment, supply rat oral gavage to use its suspension that is made into desired concn respectively with 0.5%CMC-Na.Propylthiouracil, the 50mg/ sheet. Shenzhen China Associated Pharmaceutical Co., Ltd. produces. lot number 101209.
1.2 reagent
Triglyceride (TG, 20100603), cholesterol (TC, 20100918), low-density lipoprotein (LDL-C, 20100601). high low-density lipoprotein (HDL-C, 20100723) is measured kit, and Shanghai Kehua Bio-engineering Co., Ltd produces; Cholesterol (20101103) and natrii tauroglycocholas (20110201) biochemical reagents are produced by extensive and profound in meaning star biochemical technology Co., Ltd of Shanghai biochemical technology Co., Ltd and Beijing respectively.
1.3 animal
The complete male SD rat of SPF level, body weight 180-220g is available from Disease Prevention Control Center, Hubei Prov.
1.4 instrument
The 21VtS-1024 automatic clinical chemistry analyzer, Tokyo Co., Ltd.;
AL104 type electronic analytical balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd.;
JJ2000 type electronic balance.Changshu City two outstanding tester factory;
Centrifuge5810-R desk type high speed refrigerated centrifuge, German eppendorf company;
1500 ELIASAs, Finland Lei Bo company.
1.5 statistics
The result representes with
; The normal distribution data is checked with t, and skewness distributes and uses rank test.
2 methods
2.1 divide into groups and model copy
70 of male SD rats are after laboratory adaptability raised for 1 week. and get hematometry serum TC and TG, be divided into 7 groups at random according to blood lipid level and body weight: the normal control group; Model group; The fenofibrate group; Vine tea tea in bag experimental group; The basic, normal, high dose groups of vine tea tea in bag; Every group 10.Except that the normal control group was fed normal diet, all the other each treated animals were all fed high lipid food, freely ingested and drank water.During the moulding each treated animal morning every day on an empty stomach according to dosage regularly volume of gastric infusion be 10ml/kg, normal group and model group are irritated clothes isometric(al) 0.5%CMC-Na, successive administration 42d.
2.2 index detects
Respectively experiment 0,14,28,42d takes by weighing rat body weight, gets hematometry TC and TG from intraocular corner of the eyes rear vein beard.Water 12h is can't help in fasting after the last administration, uses the yellow Jackets anesthetized animal, and arteria carotis is got blood, and the 3000rpm separation of serum is pressed kit method and measured serum TG, TC, LDL-C, HDL-C
2.3 high lipid food prescription
1% cholesterol, 10% lard, 0.5% sodium taurocholate, 5% sucrose, 0.2% propylthiouracil, 83.3% basal feed.Behind hot lard dissolving cholesterol, mixing adds water and stirs, and the kneading frustillatum is inserted in the 80-100 ℃ of baking oven and dried.
3 results
3.1, see the following form 1 to the influence of hyperlipidemia rats serum TG value:
The influence
of table 1 pair hyperlipidemia rats serum TG value
Compare with blank:
△P＜005,
△ △P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Visible by table 1; Rat fed high lipid food to the 14 days, and model group TG is significantly higher than the blank group, and maintains more stable level; Show high TG model success: vine tea compound three dose groups promptly had reduction effect in various degree to serum TG on the 14th day from administration, and are more obvious with administration effect in 42 days.
3.2, see the following form 2 to the influence of hyperlipidemia rats serum TC:
The influence
of table 2 pair hyperlipidemia rats serum TG value
Compare with blank:
△P＜0.05,
△ △P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Visible by table 2,14 days model group TC of moulding obviously increase, and all maintain metastable level to 42 days, show high TC mass formed by blood stasis moulding success; The three dose groups administrations of vine tea compound can reduce rat model serum TC level in 28 days in various degree, and are more obvious to effect in 42 days.
3.3, see the following form 3 to the influence of hyperlipidemia rats serum LDL-C and HDL-C:
The influence of table 3 couple hyperlipidemia rats serum LDL-C and HDL-C value
Compare with blank:
△P＜0.05,
△ △P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Visible by table 3,28 days model group LDL-C of moulding obviously raise, and still maintained more stable level to 42 days, of the present inventionly contained the LDL-C that vine tea compound health-care tea experimental group can reduce serum in various degree, and are best with administration effect in 42 days.
Experimental example 2:
1) specimen preparation
Get embodiment 3 (or embodiment 1,2) gained tea in bag particle 50g, boiling was filtered after 40 minutes, got filtrating, and adding physiological saline to solution amount is 100ml, promptly is to make every ml soup be equivalent to the 0.5g particle, subsequent use; 50mg captopril tablet is pulverized again, add 100ml physiological saline heating for dissolving, get supernatant after leaving standstill and make injection, promptly injection concentration is 0.5mg/ml.
2) experimental technique
With the rabbit is experimental subjects, and totally 4, body weight is about 2kg.Be equally divided into 2 groups, one group is experimental group, and one group is control group.Rabbit is cooked the arteria carotis intubate, connects pressure transducer to physiograph, and recording blood pressure changes.
Experimental group is observed the rabbit blood pressure with the extract auricular vein injection of 1ml/kg.Treat the steadily quiet notes captopril 0.25mg/kg in back of blood pressure, observe blood pressure and have or not significant change once more.
Control group is observed the rabbit blood pressure with the injection of captopril 0.25mg/kg auricular vein.Treat the steadily extract of the quiet notes in back 1ml/kg of blood pressure, observe blood pressure and have or not significant change once more.
3) experimental result
Experimental group is as shown in Figure 1: after the experimental group step-down, blood pressure gos up gradually, but the rise amplitude is less, reaches steady after about 10 minutes.But blood pressure can not return to the preceding level of injection.
Treat the steadily quiet notes captopril 0.25mg/kg in back of experimental group blood pressure, blood pressure does not have significant change once more.
Control group is as shown in Figure 2: after the control group step-down, blood pressure occurs ging up very soon, but the rise amplitude is little, reaches steady after about 4 minutes.Blood pressure recovers near level before the injection.
Treat the steadily extract of the quiet notes in back 1ml/kg of control group blood pressure, blood pressure has more obviously decline.
4) conclusion
The compound health-care tea set that contains vine tea of the present invention has the effect of quick step-down, and can keep low pressure level preferably.
Claims (8)
1. compound health-care tea that contains vine tea, its raw materials in part by weight is: vine tea tender leaf 80-200 part, the tender stem branch of vine tea 30-60 part, vine tea root 30-60 part, mulberry leaf 5-20 part, lotus leaf 5-20 part, chrysanthemum 1-10 part.
2. according to the said compound health-care tea that contains vine tea of claim 1, its raw materials in part by weight is: 150 parts of vine tea tender leafs, 40 parts in the tender stem branch of vine tea, 50 parts of vine tea roots, 10 parts on mulberry leaf, 10 parts on lotus leaf, 5 parts of chrysanthemums.
3. one kind prepares the said method that contains the compound health-care tea of vine tea of claim 1; It comprises the steps: that (1) is that the vine tea tender leaf of 80-200 part is cleaned and to be dried the back frying and complete after the cooling with parts by weight; Spray concentration is that 80% ethanol is spread the vine tea tender leaf after the system of drying in the air obtained handling after 20-30 hour out after on it, and said ethanol weight is the 8-12% of back tender leaf weight of completing; (2) be that the vine tea root of the tender stem branch of vine tea and 30-60 part of 30-60 part uses concentration to obtain concentrate as the alcohol extract of 60-80% and after concentrating with parts by weight; (3) chrysanthemum that the vine tea tender leaf after will handling and parts by weight are the mulberry leaf of 5-20 part, lotus leaf that parts by weight are 5-20 part, parts by weight are 1-10 part mix and pulverize after obtain corase meal; Above-mentioned concentrate is sprayed on said corase meal; Granulating into length through extruding is the 2-4mm particle; Again particle is carried out drying, dried particles promptly gets through can, packing after sterilization.
4. according to the said method that contains the compound health-care tea of vine tea of claim 3, it is characterized in that: frying was meant in 100-120 ℃ iron pan frying 20 minutes in the step (1).
5. according to the said method that contains the compound health-care tea of vine tea of claim 3; It is characterized in that: extracting in the step (2) is to use concentration to be heated to 40 ℃ of extractions 2 times as the ethanol of 60-80%; The each extraction 2 hours, consumption of ethanol is tender stem branch of vine tea and vine tea root weight 8 times when extracting at every turn.
6. according to the said method that contains the compound health-care tea of vine tea of claim 3, it is characterized in that: concentrating in the step (2) is that decompression is condensed into concentrate, and said concentrate is 1.05-1.10 60 ℃ of relative densities of surveying down.
7. according to the said method that contains the compound health-care tea of vine tea of claim 3, it is characterized in that: earlier tender stem branch of vine tea and vine tea undercut are processed the fritter of length at 0.5-1.0cm in the step (2).
8. according to the said method that contains the compound health-care tea of vine tea of claim 3, it is characterized in that: the said corase meal of step (3) is 10 orders, and said drying is that under 80 ℃, to be dried to moisture be below 10%, and said sterilization is through 6-8kGy dosage cobalt 60 irradiation.
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