CN102550247A - Application method of photosynthetic bacteria culture solution in protected cultivation of melon - Google Patents
Application method of photosynthetic bacteria culture solution in protected cultivation of melon Download PDFInfo
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- CN102550247A CN102550247A CN2011104507399A CN201110450739A CN102550247A CN 102550247 A CN102550247 A CN 102550247A CN 2011104507399 A CN2011104507399 A CN 2011104507399A CN 201110450739 A CN201110450739 A CN 201110450739A CN 102550247 A CN102550247 A CN 102550247A
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- photosynthetic bacteria
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- melon
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides an application method of photosynthetic bacteria culture solution in protected cultivation of melon, which comprises the steps of: diluting the photosynthetic bacteria culture solution, uniformly soaking seeds with the solution, pouring root and spraying. The method can improve the abilities of melon cultured in protected field for resisting diseases, insects and adversity, enhance photosynthetic intensity, and promote healthy growth of the melon; increases content of soluble sugar, improves quality deterioration due to using fertilizers and pesticides during artificial cultivation; degrades residual pesticides and fertilizers, and other toxic and harmful substances in soil and plant, and guarantees security of agriculture products; and effectively increases the content of organic matter in soil and saves 1/3 of fertilizer use amount through carbon fixation and nitrogen fixation of melon itself. The method disclosed in the invention cannot only decrease the use amount of pesticides and fertilizers in protected cultivation of the melon, but also cannot cause residue of any harmful substance, and can satisfy production need of standard melon of green food and organic food.
Description
Technical field
The invention belongs to muskmelon culture technique field, the application method of particularly a kind of photosynthetic bacteria culture fluid in muskmelon protection ground cultivation.
Background technology
Muskmelon is claimed sweet melon or muskmelon again, in the cultivation history in existing more than 2000 year of China, originates in African tropical desert area, after the Middle East, the Central Asia, India are imported in Egypt, imports China into after again.Muskmelon is because of sweet the gaining the name of distinguishing the flavor of, because delicate fragrance is attacked the people so have another name called muskmelon, fruit is fragrant and sweet, is rich in sugar, starch, also has a small amount of protein, mineral matter and other vitamin.Because its distinctive fragrance and tasty and refreshing mouthfeel, it becomes the snacks fruit that summer, people liked with muskmelon.About 400,000 hectares of present national muskmelon area under cultivation, 1,200 ten thousand tons of gross yields.Improving constantly of Along with people's living standard in recent years, muskmelon also begins to adopt facilities such as booth, greenhouse to cultivate with protecting, and the year-round supply of muskmelon is become a reality.
In the development of muskmelon protection ground cultivation large tracts of land, the defective of its existence and deficiency also exposed day by day are come out.The one, owing to have a moderate climate in the green house, make insect be difficult to rely on arctic weather to be eliminated, add that indoor hot and humid weather very easily brings out the several diseases insect pest of muskmelon, the conventional control method is all sprayed insecticide.Along with the drug-fast continuous enhancing of damage by disease and insect to conventional pesticide, the frequency of utilization of agricultural chemicals improves, and dosage strengthens, so that causes that the edible safety of muskmelon more and more is a problem on the market.The 2nd, because the closed environment of protection facility, illumination in the facility plastic greenhouse, carbonic acid gas wretched insufficiency, the crop photosynthesis effect is suppressed, and has influenced growing of muskmelon plant, thereby causes the dual reduction of muskmelon output and quality.The 3rd, in order to pursue unilateral economic benefit; One or two kind of crop such as muskmelon only planted at the plantation family throughout the year in green house, think little of the crop rotation with other vegetable crop, and the soil germ is increased year by year; Soil-borne disease increases the weight of every year, has seriously restricted the development of muskmelon protection ground cultivation.
At present, researchers apply bio-fertilizer on agricultural, and bio-fertilizer has nontoxic, improves the soil, characteristics free from environmental pollution.Utilize photosynthetic bacteria (Photosynthetic Bacteria; Abbreviation PSB) the photosynthetic bacteria culture fluid of producing promptly is a kind of bio-fertilizer; It is to be that raw material, active photosynthetic bacteria are bacterial classification with the autogamy medium, a kind of plyability bio-fertilizer that develops through modern biotechnology.Because it can promote plant growth, enhancing crop defending and fighting against diseases ability, degrading pesticide residues and fixing poisonous and harmful heavy metallic can promote soil substance to transform again, increase soil fertility, so crops are used its preparation, can receive the effect of increasing of quality and production.Through the existing documents and materials of retrieval, there is not public technology to relate to the report that the photosynthetic bacteria culture fluid is used as yet in muskmelon protection ground cultivation.
Summary of the invention
The purpose of this invention is to provide the application method of a kind of photosynthetic bacteria culture fluid in muskmelon protection ground cultivation, can satisfy the production needs of pollution-free food, organic food standards muskmelon.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is following:
The application method of a kind of photosynthetic bacteria culture fluid in muskmelon protection ground cultivation may further comprise the steps:
⑴ insert 1/10-1/5 photosynthetic bacteria seed culture fluid in the photosynthetic bacteria culture medium through sterilization, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 3-10 days under the 800lux – 2000lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml;
⑵ be diluted with water to 20-100 times of original volume with the photosynthetic bacteria culture fluid, and seed soaking is 10-60 minutes before the muskmelon sowing;
⑶ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, final singling or transplant after, with the usage amount of every mu of muskmelon l-10kg with the filling root that waters;
⑷ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, evenly sprays vegetative period 1 time, expanding stage 2 times with the usage amount of every mu of each l-10kg of muskmelon;
Photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any.
The photosynthetic bacteria culture medium of said inoculation photosynthetic bacteria, it consists of:
Sodium acetate 1000-2000mg CaCl2.2H2O 50-100 mg
MgSO4.7H2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K2HPO4 500-1500 mg
(NH4)2SO4 1000-2000 mg KH2PO4 400-1000 mg
FeSO4. 7H2O 5-15mg trace element solution 1-5m1
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H3BO3 200-300 mg Na2MoO4.2H2O 50-100 mg
CuSO4 2-10 mg MnSO4. 4H2O 150-250 mg
ZnSO4.7H2O 20-30 mg deionized water 100-200 ml
Compared with prior art, the present invention has following beneficial effect:
The first, adopt the photosynthetic bacteria culture fluid to improve disease-resistant, pest-resistant, the degeneration-resistant border ability of protection ground muskmelon, strengthen photosynthetic intensity, promote protection ground muskmelon to grow, improve output;
The second, adopt the photosynthetic bacteria culture fluid to reduce the harmful components such as nitrate of protection ground muskmelon, increase soluble sugar content, improve quality decline problem because of using chemical fertilizer and agricultural chemicals to cause;
The 3rd, adopt the photosynthetic bacteria culture fluid to improve the soil, residue of pesticide, heavy-metal residual and harmful microorganism ensure agricultural product security to the harm and the pollution of muskmelon in the elimination soil;
The 4th, adopt the photosynthetic bacteria culture fluid to improve the rhizosphere nitrogenase activity, solid carbon, nitrogen fixation through himself effectively increase soil with organic matter content, save the fertilizer amount of l/3.
The 5th, photosynthetic bacteria culture fluid raw material sources of the present invention are wide, and low price can not cause any harmful substance residual, can satisfy the production needs of pollution-free food, organic food standards muskmelon.
Embodiment
Below in conjunction with embodiment the present invention is made further specific descriptions:
Embodiment 1
Press following set of dispense system photosynthetic bacteria culture medium:
Sodium acetate 1000-2000mg CaCl2.2H2O 50-100 mg
MgSO4.7H2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K2HPO4 500-1500 mg
(NH4)2SO4 1000-2000 mg KH2PO4 400-1000 mg
FeSO4. 7H2O 5-15mg trace element solution 1-5m1
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H3BO3 200-300 mg Na2MoO4.2H2O 50-100 mg
CuSO4 2-10 mg MnSO4. 4H2O 150-250 mg
ZnSO4.7H2O 20-30 mg deionized water 100-200 ml
In through the photosynthetic bacteria culture medium of sterilization, insert 1/10 photosynthetic bacteria seed culture fluid, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 10 days under the 1800lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml.
Generally go up January in area, the middle and lower reach of Yangtze River, carry out muskmelon protection ground cultivation seedling-cultivating the middle ten days, and sowing is preceding except that common high-temperature seed presoaking, again with the photosynthetic bacteria culture fluid seed soaking that is diluted to 50 times of original volumes 30 minutes.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 200 times of original volume with the filling root of irrigating the fields, consumption is 6 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 150 times of original volume, and vegetative period 1 time, expanding stage 2 times evenly spray with the usage amount of every mu of each 7kg.Evidence, disease-resistant, pest-resistant, the degeneration-resistant border ability of muskmelon obviously strengthens behind the use photosynthetic bacteria culture fluid, grows soon, and growing power is strong, and it is high to receive luminosity, compares according to plantation group output increase by 18.34%, and rate of fertilizer reduces by 1/3.
Embodiment 2
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert 1/5 photosynthetic bacteria seed culture fluid, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 8 days under the 1200lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml.
Generally go up January in area, the middle and lower reach of Yangtze River, carry out muskmelon protection ground cultivation seedling-cultivating the middle ten days, and sowing is preceding except that common high-temperature seed presoaking, again with the photosynthetic bacteria culture fluid seed soaking that is diluted to 100 times of original volumes 60 minutes.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 100 times of original volume with the filling root of irrigating the fields, consumption is 10 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 70 times of original volume, and vegetative period 1 time, expanding stage 2 times evenly spray with the usage amount of every mu of each 10kg.Evidence, disease-resistant, pest-resistant, the degeneration-resistant border ability of muskmelon obviously strengthens behind the use photosynthetic bacteria culture fluid, grows soon, and growing power is strong, and it is high to receive luminosity, compares according to plantation group output increase by 19.76%, and rate of fertilizer reduces by 1/3.
Claims (2)
1. a photosynthetic bacteria culture fluid is protected the application method in the ground cultivation at muskmelon, it is characterized in that may further comprise the steps:
⑴ insert 1/10-1/5 photosynthetic bacteria seed culture fluid in the photosynthetic bacteria culture medium through sterilization, 15 ℃-20 ℃ of temperature; Intensity of illumination is that anaerobism was cultivated 3-10 days under the 800lux – 2000lux condition, and obtaining viable count is the photosynthetic bacteria culture fluid of 108-1010 cfu/ml;
⑵ be diluted with water to 20-100 times of original volume with the photosynthetic bacteria culture fluid, and seed soaking is 10-60 minutes before the muskmelon sowing;
⑶ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, final singling or transplant after, with the usage amount of every mu of muskmelon l-10kg with the filling root that waters;
⑷ be diluted with water to 50-200 times of original volume with the photosynthetic bacteria culture fluid, evenly sprays vegetative period 1 time, expanding stage 2 times with the usage amount of every mu of each l-10kg of muskmelon.
2. the application method of photosynthetic bacteria culture fluid according to claim 1 in muskmelon protection ground cultivation, it is characterized in that: photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011104507399A CN102550247A (en) | 2011-12-29 | 2011-12-29 | Application method of photosynthetic bacteria culture solution in protected cultivation of melon |
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| CN2011104507399A CN102550247A (en) | 2011-12-29 | 2011-12-29 | Application method of photosynthetic bacteria culture solution in protected cultivation of melon |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754468A (en) * | 2016-11-15 | 2017-05-31 | 沈阳师范大学 | Microbial bacterial agent and preparation for leaf vegetables culture, application process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754468A (en) * | 2016-11-15 | 2017-05-31 | 沈阳师范大学 | Microbial bacterial agent and preparation for leaf vegetables culture, application process |
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Application publication date: 20120711 |