CN106754468A - Microbial bacterial agent and preparation for leaf vegetables culture, application process - Google Patents
Microbial bacterial agent and preparation for leaf vegetables culture, application process Download PDFInfo
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- CN106754468A CN106754468A CN201611029558.8A CN201611029558A CN106754468A CN 106754468 A CN106754468 A CN 106754468A CN 201611029558 A CN201611029558 A CN 201611029558A CN 106754468 A CN106754468 A CN 106754468A
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- bacterial agent
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
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Abstract
The invention belongs to applied technical field of the agriculture microorganism in vegetable growing, it is specifically related to a kind of for the microbial bacterial agent of leaf vegetables culture and preparation, application process, on the basis of conventional photosynthetic bacteria training fermentation nutrient solution, by different proportion addition milk, chitin, pyrolkigneous liquid, after being sufficiently mixed, secondary fermentation is carried out under conditions of illumination anaerobism.Obtained microbial bacterial agent can be used for soil activating, seed soaking, pouring root, foliage-spray in leaf vegetables production process.Microbial bacterial agent prepared by the present invention can increase the beneficial microorganism quantity in soil, increase the fertility of soil, it is possible to increase the physiological property of leaf vegetables, diseases prevention expelling parasite, while product quality is improved, by increasing capacitance it is possible to increase the yield of Lettuce.
Description
Technical field
The invention belongs to applied technical field of the agriculture microorganism in vegetable growing, and in particular to one kind is used for leaf vegetables
The microbial bacterial agent and preparation, application process of vegetables culture.
Background technology
Photosynthetic bacteria is by inducing crop defence system in itself and to crop blade face, root-zone soil ring in plantation
The regulation in border improves chlorophyll content, strengthens intensity of photosynthesis, hence it is evident that suppress crop disease toxicity, fungoid, bacillary
The generation of disease；Can be obviously promoted plant growth, improve crop yield, and can effectively degrade crops and Pesticide Residue in Soil it is residual
Stay.
Chitin contains abundant C, N, can be decomposed by the microorganisms the nutrient by the use of and as plant growth.Soil can be changed
Microbiota, promote beneficial microorganism growth and suppress some phytopathogens, plant growth can be stimulated, make crops and
Fruit and vegetable increase harvest, can induce the disease resistance of plant, and immune and killing action is produced to various fungies, bacterium and virus,
There is good preventive and therapeutic effect to crop disease.
Pyrolkigneous liquid with improved soil, can prevent the damping-off of seed, can effectively suppress to hinder the microbiology class of plant growth
Breeding, and the insects such as knot nematodes can be killed.The vigor of crop root and blade can be promoted simultaneously, soil and blade is prevented and treated
Upper some pest and disease damages, promote the breeding of soil beneficial microorganism.
The content of the invention
In order to provide a kind of microbial manure of beneficial its growth to leaf vegetables, leaf to be used for the invention provides one kind
The microbial bacterial agent and preparation, application process of dish class vegetables culture, add milk, first in conventional Fermentation by Photosynthetic Bacteria nutrient solution
Shell element and pyrolkigneous liquid, assign Fermentation by Photosynthetic Bacteria nutrient solution new function, and nontoxic, noresidue, without hormone, pollution-free.
The present invention is achieved in that according to an aspect of the invention, there is provided a kind of for leaf vegetables culture
Microbial bacterial agent preparation method, comprise the following steps：
1) preparation of Fermentation by Photosynthetic Bacteria nutrient solution：Photosynthetic bacteria basal medium is prepared, photosynthetic bacteria microbial inoculum is added, adjusted
Section pH be 8.5 after carry out illumination anaerobic fermentation, be changed into cerise to zymotic fluid, obtain Fermentation by Photosynthetic Bacteria nutrient solution；
2) ratio of cumulative volume 0.1-1%, 0.1-0.5%, 0.1-0.5% is accounted for respectively according to milk, chitin, pyrolkigneous liquid
To step 1) in add milk, chitin and pyrolkigneous liquid in the Fermentation by Photosynthetic Bacteria nutrient solution for preparing, in the condition of illumination anaerobism
Under carry out secondary fermentation, the microbial bacterial agent for leaf vegetables culture is obtained after the completion of fermentation.
Further, step 1) in the volume ratio of photosynthetic bacteria basal medium and photosynthetic bacteria microbial inoculum be 9:1.
Further, step 1) fermentation condition be 27-29 DEG C of temperature, cultivate 7-9 days.
Further, step 2) in fermentation condition be 27-29 DEG C of temperature, cultivate 20-25 days.
Present invention also offers prepare according to the method described above for leaf vegetables culture microbial bacterial agent and its
Application on soil activating, seed soaking strong sprout, pouring root and foliage-spray.
In addition, present invention also offers the microbial bacterial agent for leaf vegetables culture for utilizing the above method to prepare
Application process, including following species：
1) soil activating：According to 5L/ mus of amount of application by the microbial bacterial agent for leaf vegetables culture with have
Machine fertilizer is manured into soil simultaneously, turns over rapidly, it is to avoid long-time sunlight irradiates；
2) seed soaking strong sprout：The microbial bacterial agent for leaf vegetables culture is taken, by 1：500 times of dilutions, soak dish
Kind 4-12 hour, seed is dried in the shade, the vernalization under the conditions of 22-28 DEG C after 70% seed reveals bud, is sowed at seedbed, emerged and very
Leaf start to unfold after again with 1：500 times of dilutions are sprayed once；
3) pouring root：The microbial bacterial agent for leaf vegetables culture is taken, by 1:300-500 times dilutes and punching fertilising
Mixing, pours into crop root；
4) foliage-spray：The microbial bacterial agent for leaf vegetables culture is taken, with 1:300-500 times of dilution,
Foliage-spray is carried out, spray in 7-10 days is spaced once, crop whole growth period sprays more than 3 times.
Further, in leaf vegetables growth period, according to vegetable species and growth situation, can be lived using the soil
One or more application process in change, seed soaking strong sprout, pouring root or foliage-spray.
Further, photosynthetic bacteria microbial inoculum described in preparation method is conventional photosynthetic bacteria microbial inoculum；The milk is civilian
Discarded milk；The chitin is agricultural grade chitin powder；The pyrolkigneous liquid is made charcoal for sawdust and distills institute after gained liquid stands
Obtain agricultural grade pyrolkigneous liquid.
Compared with prior art, it is an advantage of the current invention that in Fermentation by Photosynthetic Bacteria nutrient solution, adding milk, crust
Element, pyrolkigneous liquid carry out secondary fermentation, assign solution of photosynthetic bacteria new function, and nontoxic, noresidue, without hormone, pollution-free, can be
Leaf vegetables provides nutrition, the generation of disease and pest control is capable of again, while environmentally friendly safety, active component source therein
In natural ecosystems, easily decomposed or recycled by daylight, plant and various edaphons, and energy regulating crop growth,
Improve the yield and quality.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiment, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, it is not used to
Limit the present invention.
Embodiment 1,
For the preparation of the microbial bacterial agent of leaf vegetables culture
1) preparation of Fermentation by Photosynthetic Bacteria nutrient solution：Photosynthetic bacteria basal medium is prepared according to following formula：Beef extract
1.5g, peptone 2g, ammonium chloride 1g, sodium acetate 2.5g, sodium chloride 1g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, pH value 8.0-
8.5, water 1000ml, configure 180L altogether, add 20L photosynthetic bacteria microbial inoculums, and regulation pH is that illumination anaerobism is carried out at 29 DEG C after 8.5
Fermentation, culture is changed into cerise in 9 days to zymotic fluid, obtains Fermentation by Photosynthetic Bacteria nutrient solution；
2) to milk 1L, chitin 600g, pyrolkigneous liquid 400mL is added in Fermentation by Photosynthetic Bacteria nutrient solution, in illumination anaerobism
Under conditions of carry out secondary fermentation, 28 DEG C of temperature is cultivated 23 days, and the micro- life for leaf vegetables culture is obtained after the completion of fermentation
Thing microbial inoculum.
Embodiment 2,
For the preparation of the microbial bacterial agent of leaf vegetables culture
1) preparation of Fermentation by Photosynthetic Bacteria nutrient solution：Photosynthetic bacteria basal medium is prepared according to following formula：Beef extract
1.5g, peptone 2g, ammonium chloride 1g, sodium acetate 2.5g, sodium chloride 1g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, pH value 8.0-
8.5, water 1000ml, configure 180L altogether, add 20L photosynthetic bacteria microbial inoculums, and regulation pH is that illumination anaerobism is carried out at 28 DEG C after 8.5
Fermentation, culture is changed into cerise in 8 days to zymotic fluid, obtains Fermentation by Photosynthetic Bacteria nutrient solution；
2) to milk 0.3L, chitin 250g, pyrolkigneous liquid 250mL is added in Fermentation by Photosynthetic Bacteria nutrient solution, detest in illumination
Secondary fermentation is carried out under conditions of oxygen, 27 DEG C of temperature is cultivated 20 days, obtained after the completion of fermentation for the micro- of leaf vegetables culture
Bacteria agent.
Embodiment 3,
For the preparation of the microbial bacterial agent of leaf vegetables culture
1) preparation of Fermentation by Photosynthetic Bacteria nutrient solution：Photosynthetic bacteria basal medium is prepared according to following formula：Beef extract
1.5g, peptone 2g, ammonium chloride 1g, sodium acetate 2.5g, sodium chloride 1g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, pH value 8.0-
8.5, water 1000ml, configure 180L altogether, add 20L photosynthetic bacteria microbial inoculums, and regulation pH is that illumination anaerobism is carried out at 27 DEG C after 8.5
Fermentation, culture is changed into cerise in 7 days to zymotic fluid, obtains Fermentation by Photosynthetic Bacteria nutrient solution；
2) to milk 2L, chitin 1000g, pyrolkigneous liquid 1000mL is added in Fermentation by Photosynthetic Bacteria nutrient solution, detest in illumination
Secondary fermentation is carried out under conditions of oxygen, 29 DEG C of temperature is cultivated 25 days, obtained after the completion of fermentation for the micro- of leaf vegetables culture
Bacteria agent.
Embodiment 4,
Microbial bacterial agent for leaf vegetables culture prepared by embodiment 1 is applied in Lettuce cultigen：
1) soil activating：Organic base manure is first applied into field, every mu of 1 ton of ground sprays microbial bacterial agent, every mu 5 after spreading out
Rise, site preparation of turning over rapidly prevents direct sunlight；
2) seed soaking strong sprout：Take in the microbial bacterial agent of leaf vegetables culture, by 1：500 times of dilutions, soak Lettuce
Seed 4 hours, dries in the shade, and with gauze wrapped, the vernalization under the conditions of 22-28 DEG C after after 70% seed dew bud, is sowed at seedbed, strokes
Covering, prevents moisture evaporation, waits to come up, after true leaf starts to unfold, with 1：500 times of dilutions are sprayed once；
3) pouring root：The microbial bacterial agent for leaf vegetables culture is taken, by 1:300 times of dilutions mix with punching fertilising
Pour into Lettuce seedling root, every mu of punching 10 liters of amount of application of fertilising；
4) foliage-spray：The microbial bacterial agent for leaf vegetables culture is taken, with 1:300 times of dilutions, carry out oil
Wheat dish foliage-spray, once whole growth period sprays 4 times within 7-10 days at interval.
Note：In whole Lettuce production process, in addition to the above-mentioned fertilizer being previously mentioned, other any fertilizer are not used.
(data below is for influence of the microbial bacterial agent for leaf vegetables culture to Lettuce growth characteristics
Each growth characteristics data of Lettuce seedling after being soaked seed with microbial bacterial agent)：
1st, the influence sprouted to Lettuce seed：With Seed soaking as a control group, the microbial bacterial agent 1 to prepare：500
The seed soaking of times dilution is experimental group, respectively takes 100 seeds point, 5 parallel groups, carries out vernalization contrast experiment, the results are shown in Table 1：
The Lettuce percentage of seedgermination situation of table 1 is contrasted
From table 1 it follows that by after microbial bacterial agent seed soaking, Lettuce percentage of seedgermination has to a certain degree
Raising, illustrate that researched and developed microbial bacterial agent can improve the germination percentage of Lettuce seed.
1st, the influence long to Lettuce seed bud：With Seed soaking as a control group, the microbial bacterial agent 1 to prepare：500
The seed soaking of times dilution is test group, respectively takes 100 seeds point, 5 parallel groups, long to bud to be measured, and the results are shown in Table 2：
The Lettuce seed bud contrast long of table 2
From Table 2, it can be seen that long higher than control group naked oats by the Lettuce seed bud of microbial bacterial agent seed soaking
The sub- bud of colza is long.
2nd, the influence long to Lettuce seminal root：With Seed soaking as a control group, the microbial bacterial agent 1 to prepare：500
The seed soaking of times dilution is test group, respectively takes 100 seeds point, 5 parallel groups, long to the root of corresponding cultivated days to be measured, and is tied
Fruit is shown in Table 3：
The Lettuce seminal root contrast long of table 3
From table 3 it is observed that long higher than control group by the Lettuce seminal root of functional microorganism microbial inoculum seed soaking
Lettuce seminal root is long.
4th, plant strain growth situation：With Seed soaking as a control group, the microbial bacterial agent 1 to prepare：500 times of dilution immersions
It is test group to plant, and 100 seeds point, 5 parallel groups is respectively taken, under conditions of management condition is consistent, 15 days, two kinds after field planting
The Lettuce Reducing sugar of different disposal starts to occur in that notable difference, starts to harvest to the 36th day test group, Lettuce
Plant strain growth is normal, and leaf color is dark green, and cauline leaf is sturdy, without lodging phenomenon, and preserved at 0-4 DEG C do not fade within 20 days, without putrefaction；Extremely
Control group starts harvesting at the 44th day, and Lettuce plant leaf is widened, and stem is slightly thick, there is lodging phenomenon, and leaf color is yellow green.Explanation
Functional microorganism microbial inoculum can improve the physiological property of Lettuce, while product quality is improved, by increasing capacitance it is possible to increase Lettuce
Yield.
Claims (9)
1. the preparation method of the microbial bacterial agent of leaf vegetables culture is used for, it is characterised in that comprised the following steps：
1) preparation of Fermentation by Photosynthetic Bacteria nutrient solution：Photosynthetic bacteria basal medium is prepared, photosynthetic bacteria microbial inoculum is added, pH is adjusted
To carry out illumination anaerobic fermentation after 8.5, cerise is changed into zymotic fluid, obtains Fermentation by Photosynthetic Bacteria nutrient solution；
2) ratio of cumulative volume 0.1-1%, 0.1-0.5%, 0.1-0.5% is accounted for respectively to step according to milk, chitin, pyrolkigneous liquid
Milk, chitin and pyrolkigneous liquid are added in rapid 1) the middle Fermentation by Photosynthetic Bacteria nutrient solution for preparing, is entered under conditions of illumination anaerobism
Row secondary fermentation, obtains the microbial bacterial agent for leaf vegetables culture after the completion of fermentation.
2. according to the preparation method of the microbial bacterial agent for leaf vegetables culture described in claim 1, it is characterised in that
Step 1) in the volume ratio of photosynthetic bacteria basal medium and photosynthetic bacteria microbial inoculum be 9:1.
3. according to the preparation method of the microbial bacterial agent for leaf vegetables culture described in claim 1, it is characterised in that
Step 1) fermentation condition be 27-29 DEG C of temperature, cultivate 7-9 days.
4. according to the preparation method of the microbial bacterial agent for leaf vegetables culture described in claim 1, it is characterised in that
Step 2) in fermentation condition be 27-29 DEG C of temperature, cultivate 20-25 days.
5. a kind of microbial bacterial agent for leaf vegetables culture prepared according to any described methods of claim 1-4.
6. according to the microbial bacterial agent for leaf vegetables culture of any described method preparations of claim 1-4 in soil
Application on activation, seed soaking strong sprout, pouring root and foliage-spray.
7. the administration of the microbial bacterial agent for leaf vegetables culture of any described method preparations of claim 1-4 is utilized
Method, it is characterised in that including following species：
1) soil activating：Organic base manure is first applied into field, every mu of 1 ton of ground is sprayed for leaf vegetables culture after spreading out
Microbial bacterial agent, 5 liters every mu, site preparation of turning over rapidly prevents direct sunlight；
2) seed soaking strong sprout：The microbial bacterial agent for leaf vegetables culture is taken, by 1：500 times of dilutions, immersion colza 4-
12 hours, seed is dried in the shade, the vernalization under the conditions of 22-28 DEG C, after after 70% seed dew bud, is sowed at seedbed, emerges and true leaf is opened
Again with 1 after beginning to unfold：500 times of dilutions are sprayed once；
3) pouring root：The microbial bacterial agent for leaf vegetables culture is taken, by 1:300-500 times dilutes mixed with punching fertilising
Close, pour into crop root；
4) foliage-spray：The microbial bacterial agent for leaf vegetables culture is taken, with 1:300-500 times of dilution, is carried out
Foliage-spray, is spaced spray in 7-10 days once, and crop whole growth period sprays more than 3 times.
8. according to the application process of the microbial bacterial agent for leaf vegetables culture described in claim 7, it is characterised in that
In leaf vegetables growth period, according to vegetable species and growth situation, can using the soil activating, seed soaking strong sprout, pouring root or
One or more application process in foliage-spray.
9. according to the preparation method of the microbial bacterial agent for leaf vegetables culture described in claim 1, it is characterised in that
The photosynthetic bacteria microbial inoculum is conventional photosynthetic bacteria microbial inoculum；The milk is civilian discarded milk；The chitin is agricultural grade
Chitin powder；The pyrolkigneous liquid is made charcoal for sawdust and distills gained agricultural grade pyrolkigneous liquid after gained liquid stands.
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Cited By (3)
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| CN110183260A (en) * | 2019-04-26 | 2019-08-30 | 河南农业大学 | A kind of plant nutrition liquid and its application using fermented liquid for biologically producing hydrogen preparation |
| CN111165764A (en) * | 2020-03-27 | 2020-05-19 | 四川福欣食品有限公司 | Pickling solution prepared from pickle processing waste and method for processing pickle by using pickling solution |
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| CN111165764A (en) * | 2020-03-27 | 2020-05-19 | 四川福欣食品有限公司 | Pickling solution prepared from pickle processing waste and method for processing pickle by using pickling solution |
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Application publication date: 20170531 |