CN102515887A - Method for producing microorganism compound fertilizer bio-fertilizer by using sludge - Google Patents
Method for producing microorganism compound fertilizer bio-fertilizer by using sludge Download PDFInfo
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- CN102515887A CN102515887A CN2011103952633A CN201110395263A CN102515887A CN 102515887 A CN102515887 A CN 102515887A CN 2011103952633 A CN2011103952633 A CN 2011103952633A CN 201110395263 A CN201110395263 A CN 201110395263A CN 102515887 A CN102515887 A CN 102515887A
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- 244000005700 microbiome Species 0.000 title claims abstract description 33
- 239000003337 fertilizer Substances 0.000 title claims abstract description 29
- 239000010802 sludge Substances 0.000 title claims abstract description 13
- 150000001875 compounds Chemical class 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 17
- 201000010099 disease Diseases 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims description 23
- 238000002360 preparation method Methods 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 16
- 238000010025 steaming Methods 0.000 claims description 16
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- 238000002156 mixing Methods 0.000 claims description 13
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- 239000000126 substance Substances 0.000 claims description 12
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- 150000003797 alkaloid derivatives Chemical class 0.000 claims description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 7
- 208000031968 Cadaver Diseases 0.000 claims description 7
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- 229910000406 trisodium phosphate Inorganic materials 0.000 claims description 6
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- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
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- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 2
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- 238000001914 filtration Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims description 2
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Landscapes
- Fertilizers (AREA)
Abstract
The invention relates to a method for producing microorganism compound fertilizer bio-fertilizer by using sludge, which belongs to the fields of resource utilization, pollution prevention and biopesticide. Concentrated sludge and biomass straw serve as raw materials to be boiled to obtain biomass fertilizer serous fluid, bacterial strains capable of preventing crop diseases are added, disease resistance of plants improved by directly restraining pathogen growing and indirectly inducing plant defense reaction, so that requirements of crop nutrition and disease prevention are met simultaneously. Biological sludge fertilizer is produced by using materials of a large amount of sludge produced in sewage treatment and surplus biomass straws in agricultural production simultaneously by improving defensiveness of the crops on pathogenic microorganism, abandoned resources are effectively used, a large amount of land resources for filling sludge and air pollution produced by burning the biomass straws can be reduced, reasonable use of waste is obtained, and requirements of the national sustainable development strategy are met.
Description
Technical field
The present invention relates to a kind of method of utilizing mud to produce the micro organism composite fertilizer bio-feritlizer, belong to the utilization of resources, prevention and cure of pollution and field of biological pesticide.
Background technology
In recent decades; Along with becoming increasingly conspicuous of water resources imbalance between supply and demand; More and more countries is recognized sewage discharging having a strong impact on environment arbitrarily; Increasing sewage treatment facility is used in the processing of industrial and agricultural wastewater and sewage, and sludge yield increases sharply, and how handling a large amount of mud that increase year by year becomes problem demanding prompt solution in China's urban development.Source such as agricultural crop straw, wood materials industrial wood waste is wide, quantity is very big; They are the biomass organism, mainly are made up of compositions such as vegetation Mierocrystalline cellulose, semicellulose, xylogen, pectin substances, contain small amount of nitrogen, phosphorus, potassium and some trace elements; And in actual process; Often all burnt, closely do not polluted air ambient like this, and wasted resource.In plant growing process; Always follow the generation of this disease; Corps diseases not only has a strong impact on output and the quality of farm crop; Also directly be harmful to the health of people, animal, the control of corps diseases at present is main with chemical pesticide still, and excessive use chemical pesticide can make a big impact to ecological and food safety.Efficiently, low biological pesticide is the effective means that substituting chemical pesticide is produced green product.Basically all have the potential disease-resistant gene in the farming object, sometimes, these genes need be induced just and can be expressed, and the disease-resistant gene of expressing can help the harm of farm crop opposing pathogenetic bacteria and virus.The microorganism induction agent is the one type of material that can induce farm crop to produce the disease resistance defense response, and they are safety and low toxicity, green friendly mostly.In addition, be because the crop disease fungal pathogens is caused to the corps diseases great majority, through introducing biological micro-organism, can directly reduce the rate of catching an illness of farm crop to pathogenic fungi at the farm crop rhizosphere.
Summary of the invention
The objective of the invention is to merge the successful part of prior art; With thickened sludge and biomass straw is that material cooking makes the biomass fertilizers slurries; But add the corps diseases biocontrol strain; React the disease resistance that two reverse side improve plant from direct inhibition pathogen growth and indirect induction plant defense, thereby satisfy the requirement of corps nutrient and disease prevention simultaneously.
Method of the present invention is following: utilize mud to produce the method for micro organism composite fertilizer bio-feritlizer, carry out according to following step:
One, organic fertilizer is produced in the mixed boiling of mud and stalk:
(1) steaming mixed: proportional mixing thickened sludge, straw powder and steaming preparation; In high temperature and high pressure containers, external source is heated between 110 ℃ ~ 170 ℃, keeps 0.5 ~ 6 hour; Slowly forward container in the heat-processed to; So that mixture keeps mixing state, to constantly stir in the digestion process, reaction obtains the soil ulmin slurries after finishing;
(2) neutralization: add acid in the soil ulmin slurries that in step (1), obtain and be neutralized to pH=4 ~ 9, cooling is in harmonious proportion best pH=6 ~ 8, obtains the organic fertilizer work in-process after the reaction;
Two, the preparation of corps diseases biological and ecological methods to prevent plant disease, pests, and erosion microbiobacterial agent:
(1) configuration solid plate and inclined-plane LB substratum are in 30
oC cultivates bacillus subtilis strain down
Bacillus subtilisSF1 and subtilis
Bacillus subtilisJSB1 carries out activation;
(2) use LB to cultivate to the activatory thalline based on 30
oC carries out small-scale and cultivates under the condition of 150 rpm, produce microbial species liquid;
(3) will go up collected microbial species liquid of step joins in the large fermentation tank in 30
oC is cultured to senescence phase, makes genus bacillus form gemma; Contain wheat bran and the bean cake powder of 100 g/L mixture in the substratum of large fermentation tank, add the MgSO of 2 mg/L simultaneously with mass ratio 10:1
4Need during fermentation to stir, and feed through filtering sterile air;
Three, the preparation of micro organism composite fertilizer bio-feritlizer:
The microbial species liquid that (2) in the step 2 are prepared adds in the step 1 in the chilled soil ulmin slurries by the volume ratio of certain 1:50, mixes, and can make particulate state or powdery micro organism composite fertilizer after the drying, gets final product through packing after the assay was approved.
Alkaloid substance in (1) of wherein said step 1 is one or more of sodium hydroxide, Pottasium Hydroxide, sodium sulphite; The straw powder of adding is the meal of the pure one-tenth of farming rerum natura agricultural waste powder such as corn stalk, straw, grass stalk, beanstalk, sorghum stalk, peanut branches and leaves, fruit tree leaves;
The mass ratio of stalk in (1) of wherein said step 1 and mud (weight in wet base) is (1 ~ 5): 20; The consumption of steaming preparation be said mixture amount to the dry weight quality 1% ~ 8%; Steaming preparation is alkaloid substance and trisodium phosphate by mass ratio (3 ~ 10): 1 mixture formed
The acid of using in (2) of wherein said step 1 is in phosphoric acid, sulfuric acid or the nitric acid one or more, or organic acid, like in oxalic acid, Hydrocerol A or the tartrate one or more.
Yeast extract 5 g/l, peptone 10 g/l, NaCl l0 g/l in the LB substratum that uses in (1) of wherein said step 2.
Contain wheat bran and the bean cake powder of 100 g/L mixture in the fermention medium that uses in (2) of wherein said step 2, add the MgSO of 2 mg/L simultaneously with mass ratio 10:1
4
Conventional fermentation process in (1) of wherein said step 2 is liquid state fermentation or solid state fermentation; The bacterial classification of institute's fermentative prodn
Bacillus subtilisSF1 can produce tensio-active agent surfactin, induces exciting of farm crop defensive raction.And with
Bacillus subtilisJSB1 can the direct secretion antibacterial peptide, directly suppresses the growth of crop disease fungal pathogens.Both simultaneous inoculations can reduce the harm of pathogenic micro-organism to farm crop from direct inhibition pathogen growth and indirect induction farm crop defensive raction two aspects.
The present invention is to add the biological and ecological methods to prevent plant disease, pests, and erosion method of microorganism; Improve the defense of farm crop, utilized in the WWT biological straw material of surplus in the mass-produced mud and agriculture prodn simultaneously, utilize these material produce biological sludge fertilizer pathogenic micro-organism; Effectively utilized the resource of putting aside as useless; Reduce the required a large amount of land resourcess and the burning atmospheric pollution that biological straw produced of landfill mud, accomplished reasonable utilization of wastes, met the requirement of national sustainable development strategy.
Embodiment
The bacillus subtilis strain Bacillus subtilis SF1 of product tensio-active agent surfactin of the present invention and the subtilis Bacillus subtilis JSB1 that produces antibacterial peptide are available from the hundred million biotechnology Development Co., Ltds too in Danyang.
Embodiment 1
Organic fertilizer is produced in the mixed boiling of step 1, mud and stalk
(1) steaming mixed: according to the mixing quality of 1 Kg corn straw and 20 Kg mud (water ratio 70-75%); The consumption of steaming preparation is mixing 1% ~ 8% (steaming preparation is made up of by mass ratio 3:1 alkaloid substance sodium hydroxide and Digester Aid trisodium phosphate) thickened sludge, straw powder and the steaming preparation that said mixture is amounted to the dry weight quality; In high temperature and high pressure containers; External source is heated between 110 ℃ ~ 170 ℃; Kept 0.5 ~ 6 hour, and slowly forwarded container in the heat-processed to, so that mixture keeps the state that mixes.The effect of alkaloid substance is organism generation hydrolysis, decomposition, dissolving and the humify that makes in stalk and the mud, and can working in coordination with of trisodium phosphate strengthens above-mentioned effect, and the slurries that boiling is obtained homogenize more easily.The mixing of above-mentioned material does not have sequencing, as long as ratio reaches requirement, will constantly stir in the digestion process, and reaction obtains the soil ulmin slurries after finishing; The pure powdering thing of elder generation's powder when the corn straw that adds uses.
(2) neutralization: add sour bar in the soil ulmin slurries that in 1 step, obtain and be neutralized to pH=4 ~ 9; Cooling; Can use a kind of or mixing in phosphoric acid, sulfuric acid or the nitric acid, also can use organic acid, like a kind of or mixing in oxalic acid, Hydrocerol A or the tartrate; Be in harmonious proportion best pH=6 ~ 8, obtain the organic fertilizer work in-process after the reaction.
Step 2, induce the preparation of disease-resistant crops property microorganism induction agent
(1) in configuration LB substratum (yeast extract 5 g/l, peptone 10 g/l, NaCl l0 g/l), solid plate and inclined-plane, in 30
oRight under the C
Bacillus subtilisSF1 with
Bacillus subtilisJSB1 carries out activation.
(2) (wheat bran and bean cake powder mix with 10:1,100 g/L the activatory thalline to be used fermention medium; Add MgSO
42 mg/L), in 30
oC carries out small-scale and cultivates under the condition of 150 rpm, produce microbial species liquid.
(3) will go up collected microbial species liquid of step joins in the large fermentation tank in 30
oC is cultured to senescence phase, makes genus bacillus form gemma.Centrifugal collection thalline and gemma.Gemma is the hypopus of thalline, and the environment undesirable element is had very strong resistivity, biological activity more of a specified duration.
The preparation of step 3, micro organism composite fertilizer bio-feritlizer
Two kinds of microbiobacterial agents of preparation in the step 2 according to the ratio of 1:1, are added in the step 1 in the chilled soil ulmin slurries, mix, can make particulate state or powdery micro organism composite fertilizer after the drying, get final product through packing after the assay was approved.
Embodiment 2
Organic fertilizer is produced in the mixed boiling of step 1, mud and stalk
(1) steaming mixed: according to the mixing quality of 5 Kg corn straws and 20 Kg mud (water ratio 70-75%); The consumption of steaming preparation is 1% ~ 8% (steaming preparation is made up of by mass ratio 10:1 alkaloid substance sodium hydroxide and Digester Aid trisodium phosphate) that said mixture is amounted to the dry weight quality; Mix thickened sludge, straw powder and steaming preparation; In high temperature and high pressure containers, external source is heated between 110 ℃ ~ 170 ℃, keeps 0.5 ~ 6 hour; Slowly forward container in the heat-processed to, so that mixture keeps the state that mixes., the effect of alkaloid substance is organism generation hydrolysis, decomposition, dissolving and the humify that makes in stalk and the mud, and can working in coordination with of trisodium phosphate strengthens above-mentioned effect, and the slurries that boiling is obtained homogenize more easily.The mixing of above-mentioned material does not have sequencing, as long as ratio reaches requirement, will constantly stir in the digestion process, and reaction obtains the soil ulmin slurries after finishing; The pure powdering thing of elder generation's powder when the corn straw that adds uses.
(2) neutralization: add sour bar in the soil ulmin slurries that in 1 step, obtain and be neutralized to pH=4 ~ 9; Cooling; Can use a kind of or mixing in phosphoric acid, sulfuric acid or the nitric acid, also can use organic acid, like a kind of or mixing in oxalic acid, Hydrocerol A or the tartrate; Be in harmonious proportion best pH=6 ~ 8, obtain the organic fertilizer work in-process after the reaction.
Step 2, induce the preparation of disease-resistant crops property microorganism induction agent
(1) in configuration LB substratum (yeast extract 5 g/l, peptone 10 g/l, NaCl l0 g/l), solid plate and inclined-plane, in 30
oRight under the C
Bacillus subtilisSF1 with
Bacillus subtilisJSB1 carries out activation.
(2) (wheat bran and bean cake powder mix with 10:1,100 g/L the activatory thalline to be used fermention medium; Add MgSO
42 mg/L), in 30
oC carries out small-scale and cultivates under the condition of 150 rpm, produce microbial species liquid.
(3) will go up collected microbial species liquid of step joins in the large fermentation tank in 30
oC is cultured to senescence phase, makes genus bacillus form gemma.Centrifugal collection thalline and gemma.
The preparation of step 3, micro organism composite fertilizer bio-feritlizer
Two kinds of microbiobacterial agents of preparation in the step 2 according to the ratio of 1:1, are added in the step 1 in the chilled soil ulmin slurries, mix, can make particulate state or powdery micro organism composite fertilizer after the drying, get final product through packing after the assay was approved.
Composite biological fertilizer after the fermentation is pressed 120 Kg Kha
1Be used for continuous farming 3 years and contain the field planting cucumber of cucumber fusarium axysporum pathogenic bacteria.Test-results shows, increase execute this fertilizer after, the leaf area of cucumber, plant height, stem are thick, stipes number, output all significantly improve, the cucumber sickness rate significantly reduces.Compare with contrast, leaf area has increased by 10. 05%, and plant height has increased by 5.00%, and stem slightly increases by 4.70%, and the stipes number has increased by 5.60%, and output has increased by 15.55%, and the sickness rate control group is merely 20%, and control group is 80%.
Increase execute this composite fertilizer's bio-fertilizer after, soil nutrient significantly increases, soil microbial activities strengthens, and has promoted the decomposition to the soil difficultly soluble nutrient, has improved quick-acting nutrient contents in the soil.After not using the soil plantation cucumber of biological organic fertilizer, total soil nitrogen content is the 0.85g/Kg dry ground, rapid available phosphorus 101mg/ Kg dry ground, and available potassium is a 51mg/ Kg dry ground.After using the soil plantation cucumber of biological organic fertilizer, full nitrogen increases to 0.95 g/Kg dry ground, and rapid available phosphorus is 112 mg/ Kg soil, and available potassium is 56 mg/ Kg dry ground.Indexs such as full nitrogen, rapid available phosphorus, available potassium are compared with fertilizer treatment not, have improved 7.21%, 39.18%, 21.14% respectively.
Claims (5)
1. utilize mud to produce the method for micro organism composite fertilizer bio-feritlizer, it is characterized in that carrying out according to following step:
One, organic fertilizer is produced in the mixed boiling of mud and stalk:
(1) steaming mixed: proportional mixing thickened sludge, straw powder and steaming preparation; In high temperature and high pressure containers, external source is heated between 110 ℃ ~ 170 ℃, keeps 0.5 ~ 6 hour; Slowly forward container in the heat-processed to; So that mixture keeps mixing state, to constantly stir in the digestion process, reaction obtains the soil ulmin slurries after finishing;
(2) neutralization: add acid in the soil ulmin slurries that in step (1), obtain and be neutralized to pH=4 ~ 9, cooling is in harmonious proportion best pH=6 ~ 8, obtains the organic fertilizer work in-process after the reaction;
Two, the preparation of corps diseases biological and ecological methods to prevent plant disease, pests, and erosion microbiobacterial agent:
(1) configuration solid plate and inclined-plane LB substratum are in 30
oC cultivates bacillus subtilis strain down
Bacillus subtilisSF1 and subtilis
Bacillus subtilisJSB1 carries out activation;
(2) use LB to cultivate to the activatory thalline based on 30
oC carries out small-scale and cultivates under the condition of 150 rpm, produce microbial species liquid;
(3) will go up collected microbial species liquid of step joins in the large fermentation tank in 30
oC is cultured to senescence phase, makes genus bacillus form gemma; Contain wheat bran and the bean cake powder of 100 g/L mixture in the substratum of large fermentation tank, add the MgSO of 2 mg/L simultaneously with mass ratio 10:1
4Need during fermentation to stir, and feed through filtering sterile air;
Three, the preparation of micro organism composite fertilizer bio-feritlizer:
The microbial species liquid that (2) in the step 2 are prepared adds in the step 1 in the chilled soil ulmin slurries by the volume ratio of 1:50, mixes, and can make particulate state or powdery micro organism composite fertilizer after the drying, gets final product through packing after the assay was approved.
2. the method for utilizing mud to produce the micro organism composite fertilizer bio-feritlizer according to claim 1 is characterized in that alkaloid substance in (1) of wherein said step 1 is one or more of sodium hydroxide, Pottasium Hydroxide, sodium sulphite; The straw powder of adding is the meal of the pure one-tenth of corn stalk, straw, grass stalk, beanstalk, sorghum stalk, peanut branches and leaves or fruit tree leaves powder.
3. the method for utilizing mud to produce the micro organism composite fertilizer bio-feritlizer according to claim 1; It is characterized in that stalk and the mass ratio of mud weight in wet base in (1) of wherein said step 1 are (1 ~ 5): 20; The consumption of steaming preparation be said mixture amount to the dry weight quality 1% ~ 8%; Steaming preparation is alkaloid substance and trisodium phosphate by mass ratio (3 ~ 10): 1 mixture formed
The acid of using in (2) of wherein said step 1 is in phosphoric acid, sulfuric acid or the nitric acid one or more, or organic acid, like in oxalic acid, Hydrocerol A or the tartrate one or more.
4. the method for utilizing mud to produce the micro organism composite fertilizer bio-feritlizer according to claim 1, yeast extract 5 g/l, peptone 10 g/l, NaCl l0 g/l in the LB substratum that it is characterized in that using in (1) of wherein said step 2.
5. the method for utilizing mud to produce the micro organism composite fertilizer bio-feritlizer according to claim 1; Contain wheat bran and the bean cake powder of 100 g/L mixture in the fermention medium that it is characterized in that using in (2) of wherein said step 2, add the MgSO of 2 mg/L simultaneously with mass ratio 10:1
4
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