CN102507527A - 一种蜂王浆新鲜度测定的方法 - Google Patents

一种蜂王浆新鲜度测定的方法 Download PDF

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CN102507527A
CN102507527A CN2011103936965A CN201110393696A CN102507527A CN 102507527 A CN102507527 A CN 102507527A CN 2011103936965 A CN2011103936965 A CN 2011103936965A CN 201110393696 A CN201110393696 A CN 201110393696A CN 102507527 A CN102507527 A CN 102507527A
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royal jelly
pentodilysin
cml
freshness
elisa
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CN102507527B (zh
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张红城
高铁俊
刘娟
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Institute of Apicultural Research of Chinese Academy of Agricultural Sciences
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Institute of Apicultural Research of Chinese Academy of Agricultural Sciences
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Abstract

公开了一种蜂王浆新鲜度测定的新方法。该方法主要通过分别采用三维荧光光谱法和ELISA(酶联免疫吸附)方法测定蜂王浆中的糖基化末端产物Pentodilysin和CML(羧甲基赖氨酸)，来监测蜂王浆的新鲜度。迄今为止，国际上没有一个公认的可如实反映蜂王浆新鲜度并能在实际应用中推广的指标。

Description

一种蜂王浆新鲜度测定的方法
技术领域
本发明涉及到蜂王浆的新鲜度的检测。
背景技术
新鲜蜂王浆中含有丰富的生物活性物质，对人体具有很高的营养保健作用，在临床应用上取得显著疗效，如调节免疫、抗疲劳、抗衰老、抗肿瘤、改善睡眠等。对消化系统、循环系统、心脑血管系统等均有良好的辅助治疗作用，被称为“人类健康之友”。
蜂王浆的成分极其复杂，含有多种活性物质，这些活性成分对温度及其敏感，若贮存方法不当，其物理性状、化学成分、生理功能等品质会发生显著变化。因此蜂王浆的质量，关键在于新鲜度。
蜂王浆的品质与新鲜度之间存在密切的关系。目前主要采取测定蜂王浆中的蛋白质，如57KDa；蜂王浆中的酶，超氧化物歧化酶等来测定蜂王浆的新鲜度指标。但这些测定方法均存在地域差异性，检测仪器昂贵等缺陷。迄今为止，国际上没有一个公认的可如实反映蜂王浆新鲜度并能在实际应用中推广的指标。
发明内容
本发明的测定方法以荧光光谱为基础，测定蜂王浆不同储藏条件下的Pentodilysin和CML的含量。
本发明中测定的Pentodilysin和CML，是蜂王浆糖基化末端产物。蜂王浆在储存过程中，蛋白质会与糖发生非酶促褐变，即美拉德反应，且美拉德反应的程度随着储存时间的延长而加剧。其美拉德反应产物，Pentodilysin和CML的含量变化明显，可通过三维荧光光谱扫描及ELISA法检测其含量变化。
本发明中样品制备简单，Pentodilysin和CML的检测方法可靠，可作为测定蜂王浆新鲜度的方法。
具体实施方式：自行实施。

Claims (9)

1.一种蜂王浆新鲜度测定的新方法，该方法分别通过三维荧光光谱方法和ELISA(酶联免疫吸附)法，测定蜂王浆中的Pentodilysin和CML(羧甲基赖氨酸)的含量变化。
2.权利要求1中，三维荧光光谱方法是指波长扫描的方法。
3.权利要求1中，ELISA法采用的是ELISA试剂盒。
4.权利要求1中，Pentodilysin和CML(羧甲基赖氨酸)是蜂王浆储存过程中美拉德反应的产物-糖基化末端产物。
5.权利要求1中，Pentodilysin的提取方法是蜂王浆∶水∶三氯乙酸的比例为1∶5∶5，12000rpm/min，10min，取上清。
6.权利要求1中，CML的提取方法是，蜂王浆以1∶5的比例溶于水。
7.权利要求2中，波长扫描的参数为，λex：320nm，λem：380-500nm，扫描速度：240nm/min，延迟时间：0.0s。
8.权利要求1中，新鲜蜂王浆的Pentodilysin谱图为：当λex＝320nm时，新鲜王浆中Pentodilysine在λem＝445.8nm处有一个最大荧光峰，在λem＝422nm处有一个肩峰。
9.权利要求1中，新鲜蜂王浆的CML的含量为9.04ng±0.04ng/mL。 
CN201110393696.5A 2011-12-02 2011-12-02 一种蜂王浆新鲜度测定的方法 Expired - Fee Related CN102507527B (zh)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104316587A (zh) * 2014-10-20 2015-01-28 浙江大学 电泳胶蛋白条带检测蜂王浆新鲜度的建立方法和应用
CN104422677A (zh) * 2013-09-09 2015-03-18 汉圣生物科技股份有限公司 菇蕈类分析方法
CN107063978A (zh) * 2015-09-10 2017-08-18 阿自倍尔株式会社 液体中生物颗粒的检测装置及液体中生物颗粒的检测方法
CN107202778A (zh) * 2017-04-20 2017-09-26 浙江工商大学 一种青菜新鲜度的荧光检测方法
CN111665366A (zh) * 2020-06-15 2020-09-15 石城县康皇蜂业有限公司 一种用于蜂王浆的新鲜度检测方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002338598A (ja) * 2001-05-16 2002-11-27 Pola Chem Ind Inc ローヤルゼリーの鮮度の指標物質
JP2003000162A (ja) * 2001-06-21 2003-01-07 Pola Chem Ind Inc ローヤルゼリーの鮮度の鑑別法
JP3946462B2 (ja) * 2001-05-16 2007-07-18 ポーラ化成工業株式会社 ローヤルゼリーの鮮度の指標物質
CN101021484A (zh) * 2007-02-08 2007-08-22 浙江大学 一种蜂王浆新鲜度的快速检测试剂
CN101206226A (zh) * 2007-10-25 2008-06-25 中国农业科学院蜜蜂研究所 蜂王浆新鲜度检测试纸制备及应用
CN101320030A (zh) * 2008-07-21 2008-12-10 中国农业科学院蜜蜂研究所 一种测定蜂王浆新鲜度的方法
CN101363828A (zh) * 2008-09-26 2009-02-11 中国农业科学院蜜蜂研究所 一种蜂王浆品质的评价方法
CN101718702A (zh) * 2009-11-12 2010-06-02 浙江大学 一种蜂王浆新鲜度的快速检测方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002338598A (ja) * 2001-05-16 2002-11-27 Pola Chem Ind Inc ローヤルゼリーの鮮度の指標物質
JP3946462B2 (ja) * 2001-05-16 2007-07-18 ポーラ化成工業株式会社 ローヤルゼリーの鮮度の指標物質
JP2003000162A (ja) * 2001-06-21 2003-01-07 Pola Chem Ind Inc ローヤルゼリーの鮮度の鑑別法
CN101021484A (zh) * 2007-02-08 2007-08-22 浙江大学 一种蜂王浆新鲜度的快速检测试剂
CN101206226A (zh) * 2007-10-25 2008-06-25 中国农业科学院蜜蜂研究所 蜂王浆新鲜度检测试纸制备及应用
CN101320030A (zh) * 2008-07-21 2008-12-10 中国农业科学院蜜蜂研究所 一种测定蜂王浆新鲜度的方法
CN101363828A (zh) * 2008-09-26 2009-02-11 中国农业科学院蜜蜂研究所 一种蜂王浆品质的评价方法
CN101718702A (zh) * 2009-11-12 2010-06-02 浙江大学 一种蜂王浆新鲜度的快速检测方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FERRER,E ET AL.: "Fluorescence, Browning Index, and Color in Infant Formulas during Storage", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》, vol. 53, no. 12, 15 June 2005 (2005-06-15), pages 4911 - 4917 *
HARRY NURSTEN: "《The Maillard Reaction》", 31 December 2005, article "The Maillard Reaction", pages: 35 - 49 *
MORALES,FJ ET AL.: "A Study on Advanced Maillard Reaction in Heated Casein/Sugar Solutions:Fluorescence Accumulation", 《INTERNATIONAL DAIRY JOURNAL》, vol. 7, no. 11, 30 November 1997 (1997-11-30), pages 675 - 683 *
刘志强等: "ELISA法检测人血清晚期糖基化终产物及在血液透析病人的应用", 《解放军医学杂志》, vol. 25, no. 6, 31 December 2000 (2000-12-31) *
郑文华等: "美拉德反应的研究进展", 《化学进展》, vol. 17, no. 1, 31 January 2005 (2005-01-31), pages 122 - 129 *
郑火青等: "蜂王浆新鲜度指标研究浅析", 《蜜蜂杂志》, no. 4, 31 December 2006 (2006-12-31), pages 6 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104422677A (zh) * 2013-09-09 2015-03-18 汉圣生物科技股份有限公司 菇蕈类分析方法
CN104316587A (zh) * 2014-10-20 2015-01-28 浙江大学 电泳胶蛋白条带检测蜂王浆新鲜度的建立方法和应用
CN107063978A (zh) * 2015-09-10 2017-08-18 阿自倍尔株式会社 液体中生物颗粒的检测装置及液体中生物颗粒的检测方法
CN107202778A (zh) * 2017-04-20 2017-09-26 浙江工商大学 一种青菜新鲜度的荧光检测方法
CN111665366A (zh) * 2020-06-15 2020-09-15 石城县康皇蜂业有限公司 一种用于蜂王浆的新鲜度检测方法

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