CN102499340A - Probiotic preparation and preparation method thereof - Google Patents
Probiotic preparation and preparation method thereof Download PDFInfo
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- CN102499340A CN102499340A CN2011104327682A CN201110432768A CN102499340A CN 102499340 A CN102499340 A CN 102499340A CN 2011104327682 A CN2011104327682 A CN 2011104327682A CN 201110432768 A CN201110432768 A CN 201110432768A CN 102499340 A CN102499340 A CN 102499340A
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Abstract
The invention discloses a probiotic preparation and a preparation method thereof; the preparation method comprises the following steps: in aseptic environment, sterilizing a culture medium comprising, by weight, 8-12 parts of brown sugar, 8-12 parts of whey powder, 1-3 parts of glucose, 6-10 parts of peptone, 6-10 parts of yeast extract, 0.5-2 parts of potassium dihydrogen phosphate, 0.5-2 parts of magnesium sulfate, 0.5-2 parts of manganese sulfate, 3-7 parts of sodium acetate, and 60-70 parts of water at 110-120 DEG C for 30-50 min, cooling to 35-38 DEG C, inoculating 0.1-0.5 parts of probiotic strains, culturing at 35-38 DEG C for 72-84 hours to obtain the probiotic preparation. The probiotic preparation of the invention can adjust gastrointestinal tract microflora balance, promote gastrointestinal peristalsis, prevent dyspepsia, prevent alcoholic liver injury, reduce or alleviate damage of alcohol to human body, also has the functions of diarrhea prevention and immunity improvement, and is a high-quality health care drink beneficial to human health.
Description
Technical field
The present invention relates to health beverages, be specifically related to a kind of probiotics preparation and preparation method thereof.
Background technology
The injury of drinking to health is huge, and its is direct impariment of the liver not only, causes alcoholic liver, hepatitis, cirrhosis, and liver injured after, eyesight must descend, the health detoxification ability also descends, and causes immunity degradation, infects other illnesss and tumour easily.Next is drunk and injures one's stomach, and causes digesting bad, and the human body qualitative change is poor, also infects other diseases easily.In addition, drink and also can injure heart, spleen, pancreas, cause diseases such as hypertension, cardiovascular disease, apoplexy and pancreatitis easily.
Simultaneously, have loose bowels, illness such as dysentery, also make people's nutrition leak, lean and haggard, severe patient can cause intestines problem, and health is brought serious threat.
Summary of the invention
First technical problem to be solved by this invention is: provide a kind of and have antialcoholism action, can prevent alcoholic liver injured, regulate the gastrointestinal bacterial flora balance, improve the probiotics preparation of body immunity.
Second technical problem to be solved by this invention is: provide a kind of and have antialcoholism action, can prevent alcoholic liver injured, regulate the gastrointestinal bacterial flora balance, improve the preparation method of the probiotics preparation of body immunity.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A kind of probiotics preparation comprises that the culture medium fermenting process of preparing of following weight portion forms:
60～70 parts in 8～12 parts in brown sugar, 8～12 parts of whey powders, 1～3 part of glucose, 6～10 parts of peptones, 6～10 parts of yeast extracts, 0.5～2 part of potassium dihydrogen phosphate, 0.5～2 part in magnesium sulfate, 0.5～2 part of manganese sulfate, 3～7 parts of sodium acetates, 0.1～0.5 part of probio bacterial classification and water.
As a kind of preferred version, said probiotics preparation comprises that the culture medium fermenting process of preparing of following weight portion forms: 64 parts in 10 parts in brown sugar, 10 parts of whey powders, 2 parts of glucose, 8 parts of peptones, 8 parts of yeast extracts, 1 part of potassium dihydrogen phosphate, 1 part in magnesium sulfate, 1 part of manganese sulfate, 5 parts of sodium acetates, 0.2 part of probio bacterial classification and water.
As a kind of preferred, said probio bacterial classification is the EM bacterial classification.
For solving above-mentioned second technical problem, technical scheme of the present invention is:
A kind of preparation method of probiotics preparation may further comprise the steps:
In gnotobasis; By weight; The culture medium that will comprise 60～70 parts in 8～12 parts in brown sugar, 8～12 parts of whey powders, 1～3 part of glucose, 6～10 parts of peptones, 6～10 parts of yeast extracts, 0.5～2 part of potassium dihydrogen phosphate, 0.5～2 part in magnesium sulfate, 0.5～2 part of manganese sulfate, 3～7 parts of sodium acetates and water; 110～120 ℃ of sterilizations 30～50 minutes; Be cooled to 35～38 ℃ then and insert 0.1～0.5 part of probio bacterial classification, cultivated 72～84 hours, obtain said probiotics preparation at 35～38 ℃.
As a kind of optimized technical scheme, the preparation method of said probiotics preparation may further comprise the steps:
In gnotobasis; By weight; The culture medium that will comprise 64 parts in 10 parts in brown sugar, 10 parts of whey powders, 2 parts of glucose, 8 parts of peptones, 8 parts of yeast extracts, 1 part of potassium dihydrogen phosphate, 1 part in magnesium sulfate, 1 part of manganese sulfate, 5 parts of sodium acetates and water 116 ℃ of sterilizations 30 minutes, is cooled to 37 ℃ then and inserts 0.2 part of probio bacterial classification; Cultivated 72 hours at 37 ℃, obtain said probiotics preparation.
As a kind of preferred, said probio bacterial classification is the EM bacterial classification.
As a kind of preferred, employing flame vaccination ways during said accesss probio bacterial classification.
Owing to adopted technique scheme, the invention has the beneficial effects as follows:
The present invention adopts the culture medium of the various nutritional labelings that comprise brown sugar, whey powder, glucose, peptone, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, manganese sulfate, sodium acetate and water, inserts probio bacterial classification fermenting process of preparing and forms, and can regulate the gastrointestinal bacterial flora balance; Promote gastrointestinal peristalsis, the prevention indigestion increases human body to calcareous absorption; Promote the comprehensive absorption of human body to nutrition; And have symptoms such as relieving the effect of alcohol, alleviate drunk back dizziness, vomiting, can prevent alcoholic liver injury, the protection liver health; Ensure healthy, reduce or alleviation of alcohol to the injury of human body.In addition, also having and prevent to have loose bowels and improve immunity function, is the high-quality health beverages that is of value to health.
The specific embodiment
Come further to set forth the present invention below in conjunction with concrete embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
In the gnotobasis of GMP compatible, by weight, will comprise that the culture medium of 60 parts in 8 parts in brown sugar, 8 parts of whey powders, 3 parts of glucose, 10 parts of peptones, 6 parts of yeast extracts, 0.5 part of potassium dihydrogen phosphate, 2 parts in magnesium sulfate, 0.5 part of manganese sulfate, 7 parts of sodium acetates and water is put into stainless cylinder of steel; 110 ℃ of sterilizations 40 minutes; Be cooled to 35 ℃ then, adopt the flame vaccination ways, insert 0.5 part of EM bacterial classification; Cultivated 72 hours at 35 ℃, obtain said probiotics preparation.
Embodiment 2
In the gnotobasis of GMP compatible, by weight, will comprise that the culture medium of 70 parts in 12 parts in brown sugar, 12 parts of whey powders, 1 part of glucose, 6 parts of peptones, 10 parts of yeast extracts, 2 parts of potassium dihydrogen phosphates, 0.5 part in magnesium sulfate, 2 parts of manganese sulfates, 3 parts of sodium acetates and water is put into stainless cylinder of steel; 120 ℃ of sterilizations 30 minutes; Be cooled to 38 ℃ then, adopt the flame vaccination ways, insert 0.1 part of EM bacterial classification; Cultivated 72 hours at 38 ℃, obtain said probiotics preparation.
Embodiment 3
In the gnotobasis of GMP compatible, by weight, will comprise that the culture medium of 64 parts in 10 parts in brown sugar, 10 parts of whey powders, 2 parts of glucose, 8 parts of peptones, 8 parts of yeast extracts, 1 part of potassium dihydrogen phosphate, 1 part in magnesium sulfate, 1 part of manganese sulfate, 5 parts of sodium acetates and water is put into stainless cylinder of steel; 116 ℃ of sterilizations 30 minutes; Be cooled to 37 ℃ then, adopt the flame vaccination ways, insert 0.2 part of EM bacterial classification; Cultivated 72 hours at 37 ℃, obtain said probiotics preparation.
Claims (7)
1. probiotics preparation is characterized in that the culture medium fermenting process of preparing that comprises following weight portion forms:
60～70 parts in 8～12 parts in brown sugar, 8～12 parts of whey powders, 1～3 part of glucose, 6～10 parts of peptones, 6～10 parts of yeast extracts, 0.5～2 part of potassium dihydrogen phosphate, 0.5～2 part in magnesium sulfate, 0.5～2 part of manganese sulfate, 3～7 parts of sodium acetates, 0.1～0.5 part of probio bacterial classification and water.
2. probiotics preparation as claimed in claim 1 is characterized in that the culture medium fermenting process of preparing that comprises following weight portion forms:
64 parts in 10 parts in brown sugar, 10 parts of whey powders, 2 parts of glucose, 8 parts of peptones, 8 parts of yeast extracts, 1 part of potassium dihydrogen phosphate, 1 part in magnesium sulfate, 1 part of manganese sulfate, 5 parts of sodium acetates, 0.2 part of probio bacterial classification and water.
3. according to claim 1 or claim 2 probiotics preparation, it is characterized in that: said probio bacterial classification is the EM bacterial classification.
4. the preparation method of a probiotics preparation is characterized in that may further comprise the steps:
In gnotobasis; By weight; The culture medium that will comprise 60～70 parts in 8～12 parts in brown sugar, 8～12 parts of whey powders, 1～3 part of glucose, 6～10 parts of peptones, 6～10 parts of yeast extracts, 0.5～2 part of potassium dihydrogen phosphate, 0.5～2 part in magnesium sulfate, 0.5～2 part of manganese sulfate, 3～7 parts of sodium acetates and water; 110～120 ℃ of sterilizations 30～50 minutes; Be cooled to 35～38 ℃ then and insert 0.1～0.5 part of probio bacterial classification, cultivated 72～84 hours, obtain said probiotics preparation at 35～38 ℃.
5. the preparation method of probiotics preparation as claimed in claim 4 is characterized in that may further comprise the steps:
In gnotobasis; By weight; The culture medium that will comprise 64 parts in 10 parts in brown sugar, 10 parts of whey powders, 2 parts of glucose, 8 parts of peptones, 8 parts of yeast extracts, 1 part of potassium dihydrogen phosphate, 1 part in magnesium sulfate, 1 part of manganese sulfate, 5 parts of sodium acetates and water 116 ℃ of sterilizations 30 minutes, is cooled to 37 ℃ then and inserts 0.2 part of probio bacterial classification; Cultivated 72 hours at 37 ℃, obtain said probiotics preparation.
6. like the preparation method of claim 4 or 5 described probiotics preparations, it is characterized in that: said probio bacterial classification is the EM bacterial classification.
7. the preparation method of probiotics preparation as claimed in claim 6 is characterized in that: adopt the flame vaccination ways during said access probio bacterial classification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011104327682A CN102499340B (en) | 2011-12-21 | 2011-12-21 | Probiotic preparation and preparation method thereof |
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| CN2011104327682A CN102499340B (en) | 2011-12-21 | 2011-12-21 | Probiotic preparation and preparation method thereof |
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| CN102499340A true CN102499340A (en) | 2012-06-20 |
| CN102499340B CN102499340B (en) | 2013-11-13 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082345A (en) * | 2014-07-24 | 2014-10-08 | 中国水产科学研究院珠江水产研究所 | Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder |
| CN106186621A (en) * | 2016-08-22 | 2016-12-07 | 安徽逻根农业科技有限公司 | Feces of livestock and poultry harmless treatment and biogas fermentation technology |
| CN108587955A (en) * | 2018-04-24 | 2018-09-28 | 陆新军 | A kind of preparation method of compound probiotic product |
| CN108936676A (en) * | 2017-05-18 | 2018-12-07 | 细胞生物技术公司 | Include the composition for decomposing alcohol or the probiotics of acetaldehyde |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100523170C (en) * | 2006-09-29 | 2009-08-05 | 杜灵广 | Combination fermentation process of clostridiumbutyricum and lactobacillus acidophilus |
| CN101948770A (en) * | 2010-08-26 | 2011-01-19 | 天津市畜牧兽医研究所 | Method for producing lactobacillus sp. probiotics |
| CN101591616B (en) * | 2008-05-27 | 2011-04-27 | 郑春梅 | Culture method of beneficial microorganism flora |
| CN102077932A (en) * | 2010-11-29 | 2011-06-01 | 哈尔滨美华生物技术股份有限公司 | Method for preparing nutritional jelly rich in highly active intestinal probiotics |
-
2011
- 2011-12-21 CN CN2011104327682A patent/CN102499340B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100523170C (en) * | 2006-09-29 | 2009-08-05 | 杜灵广 | Combination fermentation process of clostridiumbutyricum and lactobacillus acidophilus |
| CN101591616B (en) * | 2008-05-27 | 2011-04-27 | 郑春梅 | Culture method of beneficial microorganism flora |
| CN101948770A (en) * | 2010-08-26 | 2011-01-19 | 天津市畜牧兽医研究所 | Method for producing lactobacillus sp. probiotics |
| CN102077932A (en) * | 2010-11-29 | 2011-06-01 | 哈尔滨美华生物技术股份有限公司 | Method for preparing nutritional jelly rich in highly active intestinal probiotics |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082345A (en) * | 2014-07-24 | 2014-10-08 | 中国水产科学研究院珠江水产研究所 | Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder |
| CN104082345B (en) * | 2014-07-24 | 2016-04-13 | 中国水产科学研究院珠江水产研究所 | A kind of aquaculture special EM bacterium powder and its preparation method and application |
| CN106186621A (en) * | 2016-08-22 | 2016-12-07 | 安徽逻根农业科技有限公司 | Feces of livestock and poultry harmless treatment and biogas fermentation technology |
| CN108936676A (en) * | 2017-05-18 | 2018-12-07 | 细胞生物技术公司 | Include the composition for decomposing alcohol or the probiotics of acetaldehyde |
| CN108587955A (en) * | 2018-04-24 | 2018-09-28 | 陆新军 | A kind of preparation method of compound probiotic product |
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| CN102499340B (en) | 2013-11-13 |
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Effective date of registration: 20210412 Address after: 050000 apartment 1-4-402, Tianshan Science Park, 319 Xiangjiang Road, high tech Zone, Shijiazhuang City, Hebei Province Patentee after: Hebei Yuanmin Biotechnology Co.,Ltd. Address before: No.37 Renmin Road, Wulian County, Rizhao City, Shandong Province 262300 Patentee before: Wang Jianpeng |
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