CN102461652B - 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 - Google Patents
盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 Download PDFInfo
- Publication number
- CN102461652B CN102461652B CN2010105422783A CN201010542278A CN102461652B CN 102461652 B CN102461652 B CN 102461652B CN 2010105422783 A CN2010105422783 A CN 2010105422783A CN 201010542278 A CN201010542278 A CN 201010542278A CN 102461652 B CN102461652 B CN 102461652B
- Authority
- CN
- China
- Prior art keywords
- citrus
- percent
- oranges
- tangerines
- fresh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003755 preservative agent Substances 0.000 title claims abstract description 34
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 29
- 241000207199 Citrus Species 0.000 title claims abstract description 25
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 title claims abstract description 16
- 230000002335 preservative effect Effects 0.000 title abstract 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 6
- 239000005781 Fludioxonil Substances 0.000 claims abstract description 4
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000417 fungicide Substances 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 241000675108 Citrus tangerina Species 0.000 claims description 52
- 230000000844 anti-bacterial effect Effects 0.000 claims description 11
- 239000003899 bactericide agent Substances 0.000 claims description 8
- PZBPKYOVPCNPJY-UHFFFAOYSA-N 1-[2-(allyloxy)-2-(2,4-dichlorophenyl)ethyl]imidazole Chemical compound ClC1=CC(Cl)=CC=C1C(OCC=C)CN1C=NC=C1 PZBPKYOVPCNPJY-UHFFFAOYSA-N 0.000 claims description 6
- 239000005795 Imazalil Substances 0.000 claims description 6
- 229960002125 enilconazole Drugs 0.000 claims description 6
- 150000002460 imidazoles Chemical class 0.000 claims description 6
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 claims description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 5
- 239000005820 Prochloraz Substances 0.000 claims description 5
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 5
- 150000002170 ethers Chemical class 0.000 claims description 5
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical group C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 claims description 5
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 5
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims description 3
- 239000005784 Fluoxastrobin Substances 0.000 claims description 3
- 150000001412 amines Chemical class 0.000 claims description 3
- 229910052731 fluorine Inorganic materials 0.000 claims description 3
- 239000011737 fluorine Substances 0.000 claims description 3
- UFEODZBUAFNAEU-NLRVBDNBSA-N fluoxastrobin Chemical compound C=1C=CC=C(OC=2C(=C(OC=3C(=CC=CC=3)Cl)N=CN=2)F)C=1C(=N/OC)\C1=NOCCO1 UFEODZBUAFNAEU-NLRVBDNBSA-N 0.000 claims description 3
- 230000002070 germicidal effect Effects 0.000 claims description 3
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 229940051841 polyoxyethylene ether Drugs 0.000 abstract description 4
- 229920000056 polyoxyethylene ether Polymers 0.000 abstract description 4
- 230000000855 fungicidal effect Effects 0.000 abstract 2
- 239000005730 Azoxystrobin Substances 0.000 abstract 1
- 239000005828 Pyrimethanil Substances 0.000 abstract 1
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 abstract 1
- ZLIBICFPKPWGIZ-UHFFFAOYSA-N pyrimethanil Chemical compound CC1=CC(C)=NC(NC=2C=CC=CC=2)=N1 ZLIBICFPKPWGIZ-UHFFFAOYSA-N 0.000 abstract 1
- 125000001453 quaternary ammonium group Chemical group 0.000 abstract 1
- 235000021552 granulated sugar Nutrition 0.000 description 24
- 235000013399 edible fruits Nutrition 0.000 description 21
- 238000003756 stirring Methods 0.000 description 15
- 241000159512 Geotrichum Species 0.000 description 12
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 8
- 241000453701 Galactomyces candidum Species 0.000 description 6
- 235000017388 Geotrichum candidum Nutrition 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 5
- 230000004763 spore germination Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- BCPFWSWROVXGQA-UHFFFAOYSA-N 2-(2,4,4-trimethylpentan-2-yl)guanidine Chemical compound CC(C)(C)CC(C)(C)N=C(N)N BCPFWSWROVXGQA-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 239000004359 castor oil Substances 0.000 description 3
- 235000019438 castor oil Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002389 environmental scanning electron microscopy Methods 0.000 description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 3
- 229950000289 guanoctine Drugs 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- ZAIRCVNISFRBHW-UHFFFAOYSA-N 1h-benzimidazol-2-yl n-methylcarbamate Chemical group C1=CC=C2NC(OC(=O)NC)=NC2=C1 ZAIRCVNISFRBHW-UHFFFAOYSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- -1 Polyhexamethylene guanidine Polymers 0.000 description 1
- 229920002413 Polyhexanide Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 229920006317 cationic polymer Polymers 0.000 description 1
- 238000009371 citriculture Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 201000010251 cutis laxa Diseases 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Substances NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- 229940083094 guanine derivative acting on arteriolar smooth muscle Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- CBFCDTFDPHXCNY-UHFFFAOYSA-N octyldodecane Natural products CCCCCCCCCCCCCCCCCCCC CBFCDTFDPHXCNY-UHFFFAOYSA-N 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L71/00—Compositions of polyethers obtained by reactions forming an ether link in the main chain; Compositions of derivatives of such polymers
- C08L71/02—Polyalkylene oxides
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L79/00—Compositions of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen or carbon only, not provided for in groups C08L61/00 - C08L77/00
- C08L79/04—Polycondensates having nitrogen-containing heterocyclic rings in the main chain; Polyhydrazides; Polyamide acids or similar polyimide precursors
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Nutrition Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂。该柑桔保鲜剂，按总质量分数100%计，由0.05%～0.5%盐酸聚六亚甲基胍，0.05%～0.2%咪唑类杀菌剂、苯并咪唑类杀菌剂、嘧霉胺、氟咯菌腈、嘧菌酯中的一种或数种的混合物，0.02%～0.15%聚氧乙烯醚类或季胺盐类乳化剂，以及余量的水组成。本发明的柑桔保鲜剂能明显抑制沙糖桔等柑桔类果实在贮藏期间腐烂，特别是酸腐病的发生。如沙糖桔果实用本发明的柑桔保鲜剂处理后在常温（15～25）贮藏45天后，酸腐病发病率低于10％，而对照高达60％，因此本发明的柑桔保鲜剂显著的降低宽皮桔类在贮藏过程中酸腐病的发生，大大提高了柑桔的经济价值。
Description
技术领域
本发明属于水果保鲜领域，具体来说涉及盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂。
背景技术
柑桔是世界第一大水果，有138个国家栽培，面积约667万公顷，年总产量1亿余吨，约占世界水果总产量的22%。据统计，2008年我国柑橘栽培面积达3045万亩，总产量2331万吨，占世界柑桔产量约23%，为世界柑桔第一生产大国。广东省是我国柑桔主产区之一，沙糖桔为我省柑桔品种中最具特色的品种，在国内外水果市场上极具竞争力。然而由于沙糖桔独特的生物学特性，与柑桔类其它水果相比难保鲜，采后损耗相当严重，每年的损失都在数亿元以上，严重制约沙糖桔产业的发展。
柑桔属非呼吸跃变型果实，但采后呼吸代谢旺盛导致营养与风味品质迅速下降。沙糖桔皮薄汁多，采后病害发生严重，更不耐贮藏。沙糖桔贮藏期间发生的主要病害有酸腐病、青绿霉病、黑腐病和蒂腐病。目前，控制沙糖桔果实病害的最有效的途径是低温贮藏结合化学杀菌剂处理，所用的杀菌剂有多菌灵、甲基托布津、抑霉唑、双胍盐（百可得）等，但对腐烂特别是酸腐病的控制方面仍不够理想。
酸腐病是目前沙糖桔贮运中最常见、最难防治的病害之一，其致病菌为半知菌亚门丝孢纲内的白地霉（Geotrichum candidum），病菌从伤口侵入。酸腐病只危害果实，一般发生于成熟或过熟果，特别是贮藏期的果实。酸腐病的发病率受采前栽培措施、成熟度、采收方法、果实水分状况、贮运措施等多种因素影响，如广东省沙糖桔主产区2007年酸腐病发病率在10％以下，而2010年初因天气原因发病率高达50％以上。对于柑桔酸腐病的防治，咪唑类和苯并咪唑类防腐剂均无效。双胍盐（百可得）对防治酸腐病效果较好，但存在农药残留和安全性问题，在美国双胍盐（百可得）因致癌作用已被禁止使用。因此，寻求安全高效而又操作简单的防酸腐病技术势在必行。
PHMG（盐酸聚六亚甲基胍，Polyhexamethylene guanidine）是国际上新合成的一种绿色环保型广谱抗菌剂，PHMG属胍类衍生物，是一种阳离子聚合物。它通过作用于病原菌的细胞壁和细胞质的膜系统，与细胞膜磷脂中的脂肪酸结合，迅速击穿它们的细胞质膜，攻破病菌细胞的核心，使病菌体内的生命物质外流，从而达到最佳的杀菌效果。PHMG是高分子聚合物，不易被动物组织吸收，因而大大降低了它的毒性。据俄罗斯检疫部门的结果，PHMG对小鼠类的半致死量LD50为25000mg/kg，为实际无毒级。由于PHMG具有无毒、高效、稳定等特点，而被广泛用于医院消毒、饮用水和食品加工设备的消毒。PHMG对细菌和病毒有较强的杀灭及抑制作用，对真菌抑制作用的研究和应用较少。目前，未有PHMG应用于采后果蔬真菌病害研究和应用的报道。
发明内容
本发明的第一个目的是提供盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用。
本发明通过对柑桔采后酸腐病致病菌白地霉Geotrichum candidum离体和活体研究，筛选到了一种能明显抑制白地霉Geotrichum candidum生长，有效降低沙糖桔果实酸腐病发生抗菌物质－盐酸聚六亚甲基胍，结合防治青绿霉病杀菌剂，得到的保鲜剂能有效抑制宽皮桔贮藏和流通过程中腐烂，特别是酸腐病的发生。
因此本发明的第二个目的是提供一种专门针对柑桔采后腐烂，特别是控制酸腐病发生的柑桔保鲜剂。
本发明的柑桔保鲜剂，按总质量分数100%计，由0.05%～0.5% 盐酸聚六亚甲基胍，0.05%～0.2% 咪唑类杀菌剂、苯并咪唑类杀菌剂、嘧霉胺、氟咯菌腈、嘧菌酯中的一种或数种的混合物，0.02%～0.15% 聚氧乙烯醚类或季胺盐类乳化剂，以及余量的水组成。
所述的咪唑类杀菌剂优选为咪鲜胺和抑霉唑。
所述的苯并咪唑杀菌剂优选为多菌灵、甲基托布津和特克多。
所述的盐酸聚六亚甲基胍优选质量分数为0.1%~0.5%，进一步优选为0.25%~0.5%。
本发明的柑桔保鲜剂的制备方法如下，先将乳化剂和盐酸聚六亚甲基胍混合，再加入咪唑类杀菌剂、苯并咪唑类杀菌剂、嘧霉胺、氟咯菌腈、嘧菌酯中的一种或数种的混合物作为防腐剂和少量水，搅拌均匀得到产物，使用时再加入水稀释到上述浓度而得到本发明的柑桔保鲜剂。
本发明的柑桔保鲜剂能明显抑制沙糖桔等柑桔类果实在贮藏期间腐烂，特别是酸腐病的发生。如沙糖桔果实用本发明的柑桔保鲜剂处理后在常温（15～25）贮藏45天后，酸腐病发病率低于10％，而对照高达60％，因此本发明的柑桔保鲜剂显著的降低宽皮桔类在贮藏过程中酸腐病的发生，大大提高了柑桔的经济价值。
附图说明：
图1是不同浓度PHMG对离体Geotrichum candidum孢子萌发的影响；
图2是PHMG对离体Geotrichum candidum菌丝生长的抑制（滤纸抑菌圈法）；
图3是Geotrichum candidum菌丝在含PHMG培养基上生长的扫描电镜图片。
具体实施方式：
以下是对本发明的进一步说明，而不是对本发明的限制。
下述实施例中多菌灵即2-苯并咪唑基氨基甲酸甲酯，甲基托布津即1,2-二(3-甲氧碳基-2-硫脲基)苯，特克多即2-(噻唑-4-基)苯并咪唑，咪鲜胺即N-丙基-N-[2-(2,4,6-三氯苯氧基)乙基]-咪唑-1-甲酰胺，抑霉唑即1-「2-(2,4-二氯苯基)-2-（2-烯丙氧基）」-1H-咪唑。
一、离体抑菌实验
1 材料与方法
（1）菌株：从发生酸腐病沙糖桔中分离纯化的柑桔白地霉Geotrichum candidum.
（2）孢子萌发试验：采用孢子液法，将计算好的一定量PHMG药剂加入已灭菌的1/2 PBD培养基中，配制成9个浓度梯度：0，0.1，1，2，5，10，20，50，100 mg/L。分别取5ml含不同浓度PHMB的培养基至10 ml已灭菌的试管中，加入0.1ml 已配制好的标准孢子悬浮液，于26℃恒温条件下250rpm/min振荡培养，培养8小时后取出1-2菌液，显微镜下观察，测定孢子萌发数，统计孢子萌发率。
（3）菌丝生长试验：采用滤纸抑菌圈法，把已配好并经过高压蒸汽灭菌的PDA培养基冷却45-50 °C，加入Geotrichum candidum孢子悬浮液至培养基中，孢子浓度为106个/L，将培养基倒入已灭菌的培养皿中制成平板。取直径5 mm的灭菌滤纸片放入平板中央，在滤纸上加入10μl 1g/L的PHMG溶液（用无菌水代替PHMG作为对照），然后在26℃恒温培养箱内培养5天，测量滤纸抑菌圈的直径并进行分析。
（4）菌丝体扫描电镜观查：将已配好含有10mg/L PHMG并经高压蒸汽灭菌的PDA培养基冷却至45-50 °C，倒入已灭菌的培养皿中制成平板，接种上Geotrichum candidum孢子悬浮液，在26℃恒温培养箱内培养5天，用直径为0.4 cm 的打孔器在上述菌落边缘切下菌丝体按常规方法进行扫描电镜观查。
试验结果
如图1所示，PHMG显著抑制了Geotrichum candidum离体孢子萌发，并且表现出浓度效应，当PHMG的浓度为5 mg/L时，Geotrichum candidum孢子萌发率仅为2.13%，而10mg/L PHMG则几乎完全抑制了沙糖桔Geotrichum candidum孢子萌发。滤纸抑菌圈法试验表明，含10μl 1g/L PHMG的滤纸片对Geotrichum candidum菌丝生长显著的抑制作用，在26℃培养培养5天后，抑菌圈直径为2.19 cm (图2)。从图3可看出，在不含PHMG的PDA培养基，Geotrichum candidum菌丝生长正常，菌丝呈棒状、均匀、表面光滑，而在含PHMG培养基上生长的菌丝，菌丝体严重扭曲，表面凹陷，进一步证实了PHMG对菌丝伸长的抑制作用。
综上所述，盐酸聚六亚甲基胍（PHMG）能应用于防治柑桔酸腐病上。
二、柑桔保鲜剂
实施例1：
将PHMG 5g和聚季铵盐-7 2g混合，然后再加入多菌灵10g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例2：
将PHMG 25g和二癸基二甲基氯铵2g混合，再加入甲基托布津10g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例3：
将PHMG 50g和蓖麻油聚氧乙烯醚类农乳BY120 15g混合，再加入特克多20g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例4：
将PHMG 10g和十二烷基三甲基氯化铵4g混合，再加入咪鲜胺10g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例5：
将PHMG 10g和壬基酚聚氧乙烯醚农乳100号5g混合，再加入抑霉唑10g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例6：
将PHMG 5g和蓖麻油聚氧乙烯醚类农乳剂BY120 5g，再加入特克多5g、抑霉唑5g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
实施例7：
将PHMG 40g和蓖麻油聚氧乙烯醚类乳化剂 EL-80 12g，再加入咪鲜胺5g和少量水，搅拌均匀，最后加水至总重量为10 kg，搅拌均匀，得到本实施例的柑桔保鲜剂，用该柑桔保鲜剂浸泡沙糖桔整果。
本发明柑桔保鲜剂的实施效果试验
按上述实施例1～7配制保鲜剂。挑选适期采收、无病虫害和机械伤的沙糖桔果实，在上述保鲜剂中分别浸泡30秒后稍晾干，以不做浸泡处理的沙糖桔果实作对照。各实验组和对照组的沙糖桔都用0.03mm厚的聚乙烯薄膜袋包装，置于塑料筐中，每筐15kg，常温(25℃) 贮藏45天，统计各组的腐烂情况，结果见表1。
表1. 本发明的保鲜剂对常温贮藏的沙糖桔的保鲜效果
| 组名 | 酸腐病发生率（%） | 腐烂率（％） |
| 实施例1 | 7.6 | 20 |
| 实施例2 | 0.7 | 3.7 |
| 实施例3 | 0 | 4.4 |
| 实施例4 | 4.5 | 9.1 |
| 实施例5 | 3.3 | 8.4 |
| 实施例6 | 8.3 | 10.6 |
| 实施例7 | 0.5 | 6.5 |
| 对照组 | 60.1 | 85.8 |
Claims (6)
1.盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用。
2.一种柑桔保鲜剂，其特征在于，按总质量分数100%计，由0.05%～0.5% 盐酸聚六亚甲基胍，0.05%～0.2% 咪唑类杀菌剂、苯并咪唑类杀菌剂、嘧霉胺、氟咯菌腈、嘧菌酯中的一种或数种的混合物，0.02%～0.15% 聚氧乙烯醚类或季胺盐类乳化剂，以及余量的水组成。
3.根据权利要求2所述的柑桔保鲜剂，其特征在于，所述的盐酸聚六亚甲基胍的质量分数为0.1%~0.5%。
4.根据权利要求3所述的柑桔保鲜剂，其特征在于，所述的盐酸聚六亚甲基胍的质量分数为0.25%~0.5%。
5.根据权利要求2、3 或4 所述的柑桔保鲜剂，其特征在于，所述的咪唑类杀菌剂为咪鲜胺和抑霉唑。
6.根据权利要求2、3 或4 所述的柑桔保鲜剂，其特征在于，所述的苯并咪唑杀菌剂为多菌灵、甲基托布津和特克多。
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105422783A CN102461652B (zh) | 2010-11-14 | 2010-11-14 | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 |
| US13/885,165 US9192162B2 (en) | 2010-11-14 | 2010-11-15 | Use of polyhexamethylene guanidine hydrochloride as preservative to control citrus sour rot and its application |
| PCT/CN2010/078754 WO2012062001A1 (zh) | 2010-11-14 | 2010-11-15 | 盐酸聚六亚甲基胍作为防治柑桔酸腐病的保鲜剂及其应用 |
| US14/881,164 US20160029632A1 (en) | 2010-11-14 | 2015-10-13 | Citrus preservative of polyhexamethylene guanidine hydrochloride |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105422783A CN102461652B (zh) | 2010-11-14 | 2010-11-14 | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102461652A CN102461652A (zh) | 2012-05-23 |
| CN102461652B true CN102461652B (zh) | 2013-05-22 |
Family
ID=46050329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105422783A Active CN102461652B (zh) | 2010-11-14 | 2010-11-14 | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US9192162B2 (zh) |
| CN (1) | CN102461652B (zh) |
| WO (1) | WO2012062001A1 (zh) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102461652B (zh) * | 2010-11-14 | 2013-05-22 | 中国科学院华南植物园 | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 |
| CN103976015B (zh) * | 2014-05-13 | 2016-04-06 | 珠海真绿色技术有限公司 | 一种用于柑橘蒂腐病防治的含有聚氨丙基双胍的保鲜剂及其制备和应用 |
| CN104186524B (zh) * | 2014-09-02 | 2016-06-08 | 海南正业中农高科股份有限公司 | 含聚六亚甲基双胍或其盐的杀菌组合物 |
| AU2016325202A1 (en) * | 2015-09-25 | 2018-05-17 | Flechsig Patent Company Llc | Novel plant protecting compositions and uses thereof |
| CN105285083A (zh) * | 2015-11-09 | 2016-02-03 | 中国科学院华南植物园 | 一种水蜜桃贮藏保鲜方法 |
| CN107006596A (zh) * | 2017-04-18 | 2017-08-04 | 广西南宁乐蕊生物科技有限责任公司 | 一种沙糖桔保鲜剂 |
| AT521125A1 (de) * | 2018-03-23 | 2019-10-15 | Bcsk Biocid Gmbh | Flüssige Zusammensetzung |
| CN110384090B (zh) * | 2019-08-28 | 2020-07-24 | 广西壮族自治区农业科学院 | 用于促进果蔬机械损伤伤口愈合的愈合剂、使用方法和应用 |
| CN113080248B (zh) * | 2021-05-18 | 2022-09-13 | 华中农业大学 | 柑橘采后快速验伤及同时抑菌的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4026473A1 (de) * | 1990-08-22 | 1992-02-27 | Basf Ag | Bis-guanidine und diese enthaltende fungizide |
| CN1390876A (zh) | 2002-07-26 | 2003-01-15 | 于锋 | 杀菌剂原料盐酸聚六亚甲基胍及其制备方法 |
| US20060276468A1 (en) * | 2005-05-12 | 2006-12-07 | Blow Derek P | Wood preservative formulations comprising Imazalil |
| RU2282463C1 (ru) * | 2005-11-07 | 2006-08-27 | Сергей Алексеевич Балехов | Способ изготовления дезинфицирующего средства |
| CN101156627B (zh) * | 2007-09-21 | 2011-04-27 | 华南农业大学 | 含有茶皂素的柑桔防腐保鲜剂 |
| CN101263837B (zh) * | 2008-04-22 | 2010-06-30 | 中国科学院华南植物园 | 一种沙糖桔复合保鲜剂 |
| CN102461652B (zh) * | 2010-11-14 | 2013-05-22 | 中国科学院华南植物园 | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 |
-
2010
- 2010-11-14 CN CN2010105422783A patent/CN102461652B/zh active Active
- 2010-11-15 WO PCT/CN2010/078754 patent/WO2012062001A1/zh active Application Filing
- 2010-11-15 US US13/885,165 patent/US9192162B2/en active Active
-
2015
- 2015-10-13 US US14/881,164 patent/US20160029632A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20130245079A1 (en) | 2013-09-19 |
| US20160029632A1 (en) | 2016-02-04 |
| US9192162B2 (en) | 2015-11-24 |
| CN102461652A (zh) | 2012-05-23 |
| WO2012062001A1 (zh) | 2012-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102461652B (zh) | 盐酸聚六亚甲基胍在防治柑桔酸腐病上的应用及其保鲜剂 | |
| CN102461653B (zh) | 聚六亚甲基双胍盐酸盐在防治柑桔酸腐病上的应用及其保鲜剂 | |
| Gao et al. | Research on volatile organic compounds from Bacillus subtilis CF-3: biocontrol effects on fruit fungal pathogens and dynamic changes during fermentation | |
| CN102317462B (zh) | 控制乙醇发酵方法中的微生物污染 | |
| CN104531574B (zh) | 一种解淀粉芽孢杆菌gfj‑4及其组合物 | |
| CN104982516B (zh) | 一种柑桔的绿色生态保鲜方法及保鲜剂 | |
| El-Mohamedy et al. | Application of chitosan and essential oils as alternatives fungicides to control green and blue moulds of citrus fruits | |
| CN103039491A (zh) | 一种含壳寡糖与咪鲜胺的组合物 | |
| CN101984834B (zh) | 果实病害防腐保鲜剂 | |
| Khalil Bagy et al. | Control of green mold disease using chitosan and its effect on orange properties during cold storage | |
| CN106912484B (zh) | 防食用菌细菌性腐烂病的辛菌胺醋酸盐水剂、方法及应用 | |
| CN105861340B (zh) | 一株控制柑橘和葡萄采后病害的盔形毕赤酵母 | |
| CN105454273B (zh) | 一种杀菌剂组合物 | |
| CN106359411A (zh) | 一种防控猕猴桃软腐病的复配杀菌剂及其使用方法 | |
| Liu et al. | Research progress on postharvest sweet potato spoilage fungi Ceratocystis fimbriata and control measures | |
| CN106615071B (zh) | 紫檀芪在防治葡萄灰霉病和荔枝霜疫霉病中的应用 | |
| US20220256856A1 (en) | Antifungal composition comprising natamycin and at least one insecticide | |
| CN107372556A (zh) | 一种含有吡唑醚菌酯、氨基寡糖素与噻虫嗪的悬浮种衣剂 | |
| CN104585309A (zh) | 一种枣皮提取物天然果蔬杀菌保鲜剂及其使用方法 | |
| CN112753717A (zh) | 一种抑杀植物致病真菌的植物精油纯露及其制备方法 | |
| CN105191997A (zh) | 一种含有咪鲜胺和黄芩的杀菌剂 | |
| CN105076241A (zh) | 一种含有嘧菌酯和黄芩的杀菌剂 | |
| CN1275527C (zh) | 植物源农药及其生产方法 | |
| Khalil | Effect of ozone application on postharvest quality and microbiological state of “Zaghloul” date palm fruits | |
| CN107410318A (zh) | 一种吡啶杂环季铵盐阳离子表面活性剂农用杀菌剂 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |