CN102430059B - Chinese medicinal extract composition with melanogenesis promoting effect and application thereof - Google Patents
Chinese medicinal extract composition with melanogenesis promoting effect and application thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal extract composition with a melanogenesis promoting effect and application thereof. The Chinese medicinal extract composition is prepared by compounding tall gastrodia tuber extract, loofah (fresh) extract, red bean extract, common selfheal fruit-spike extract and radish seed extract, can be used for externally applied products such as medicines for treating vitiligo, cosmetics for blackening skin and hair, and the like, can effectively activate the activity of tyrosinase and the melanin synthesis function of black cells, has a melanin synthesis promoting effect, and is a safe and high-efficiency melanogenesis promoter.
Description
Technical field
The present invention relates to a kind of composite Chinese medicine composition, said composition has the function that promotes that melanin generates, and can be applied to the exterior-applied articles such as cosmetics, pharmaceuticals of the U.S. black and black hair of therapy of vitiligo, skin.
Background technology
The color of hair and skin depends on the melanin amount in hair and the skin, when the melanin growing amount in hair and the skin is very few, will cause that poliosis generates and the vitiligo disease.Melanin is the melanocyte generation by hair ball top section and stratum basale, its forming process is: the tyrosine (tyrosine) in the melanocyte of human body skin and ball top section is oxidized generation DOPA (dopa) and DOPA quinone (dopaquinone) under the catalytic action of tryrosinase, generate dopachrome (dopachrome) through resetting laggard oxidation step, 5,6-dihydroxy indole (5,6-dihydroxyindole), istain (indole-5,6-quinone), final synthesis of melanin (melanin) is shown in its reaction accompanying drawing 1.
The principal element that affects melanic formation has: the content of the quantity of melanocyte, the activity of tryrosinase, oxygen-derived free radicals and the content of tyrosine.Wherein, tyrosine is the aminoacid of needed by human, and its content is controlled by human body self.So, control melanic formation, can only be by quantity, the activity of tryrosinase and the content of oxygen-derived free radicals of control melanocyte.The existence of oxygen-derived free radicals has very large harm to health, and it is infeasible promoting melanin to generate by the content that improves oxygen-derived free radicals.So the secure way that promotes skin and hair melanin to generate mainly contains the following aspects: the one, by activating the dermal melanin cell proliferation, increase the quantity that melanocyte generates; The 2nd, by activating the catalytic activity of key enzyme-tryrosinase that melanin generates, thereby increase melanic generating rate.
At present, many people like brown skin, generally adopt the modes such as sun tan or tanned beauty treatment to accept UVA section ultraviolet irradiation, and modern science prove, UVA section ultraviolet also has very large harm to human body skin.The most normal using method of medicine for curing poliosis is to dye blackly by hair dye, and this method can only be temporary transient blackening, and the hair dyes such as p-phenylenediamine (PPD) that use at present have larger harm to human body, even carcinogenic.So researching and developing natural safe and effective melanin promoter for generation is to have larger market potential, has caused people's interest.Therefore some researchs about promoting that melanin generates have been carried out, for example, patent of invention ZL200480012349.1 has announced a kind of Herba Lactucae Indicae extract or fatty acid feather fan alcohol ester and/or its isomer of containing as short melanin generation agent and the compositions thereof of effective ingredient.Patent of invention CN1764467 has announced a kind of black hair liquid formulation and preparation method of the poliosis reincarnation hair color that is comprised of Cordyceps, Radix Ginseng, the Radix Astragali, Aloe, Typhonium flagelliforme (Lodd.) Blume, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Herba speranskiae tuberculatae, Cortex Dictamni, Semen Cuscutae, Rhizoma Et Radix Notopterygii, Fructus Chaenomelis, Radix Sophorae Flavescentis, Radix Saposhnikoviae, Herba Schizonepetae, Rhizoma Drynariae, the Radix Polygoni Multiflori, Cacumen Platycladi, Herba Ecliptae, white Peony Root.Patent of invention CN102119991A has announced a kind of compositions with black hair additional issue effect that contains Aloe, Semen Fagopyri Esculenti, Radix Polygoni Multiflori, citric acid.Patent of invention ZL200510102137.9 has announced a kind of prescription and preparation method that contains the black hair preparation of Gallus Domesticus, Aloe, Radix Ginseng, male Bombycis mori, Herba Cistanches, Radix Salviae Miltiorrhizae, Herba Epimedii, taurine, Testis et penis callorhini, Herba Schizonepetae, Mel, Radix Saposhnikoviae, Radix Polygoni Multiflori, sucrose, Ziziphi Spinosae, Formica fusca.Patent of invention ZL200910306893.1 has announced a kind of leukodermic outer wiping Chinese medicine composition for the treatment of that is comprised of Radix Polygoni Multiflori, Fructus Atriplicis Sibiricae, Radix Angelicae Sinensis, Radix Paeoniae Rubra, Flos Carthami, Caulis Spatholobi, the Radix Angelicae Dahuricae, Semen Sesami Nigrum, Fructus Psoraleae, Testa sojae atricolor.Patent of invention CN101623330 has announced a kind of leukodermic outer wiping Chinese medicine composition for the treatment of that is comprised of Fructus Psoraleae, Fructus Atriplicis Sibiricae, Radix Saposhnikoviae, common fig leaf juice (fresh).Patent of invention CN101502577 has announced a kind of leukodermic Chinese medicine external medicinal liquid for the treatment of that is comprised of Fructus Mume, Semen Cuscutae, Fructus Psoraleae, Flos Carthami and preparation method thereof.
The invention provides a kind of compositions that contains Rhizoma Gastrodiae extract, Fructus Luffae (fresh) extract, Semen Phaseoli extract, Spica Prunellae extract, Semen Raphani extract, said composition energy effective stimulus melanocyte is hyperfunction, activity of tyrosinase promotes melanic generation.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese medicine extraction compositions, it is hyperfunction that this Chinese medicine extraction compositions can activate melanocyte, and activity of tyrosinase promotes melanic generation, have the function of black hair, U.S. casting skin, and have the advantages such as stable in properties and zest be little.
Another object of the present invention is that this Chinese medicine extraction compositions is used for the U.S. black cosmetics of skin, makes stable in properties, respond well black cosmetics of U.S..
Another object of the present invention is that this Chinese medicine extraction compositions is used for skin therapy of vitiligo pharmaceuticals, makes stable in properties, respond well therapy of vitiligo pharmaceuticals.
Another object of the present invention is that this Chinese medicine extraction compositions is used for the black hair cosmetics, makes stable in properties, respond well black hair cosmetics.
To achieve the object of the present invention, the present invention has adopted following technical scheme:
A kind of have a Chinese medicine extraction compositions that promotes the melanin systematic function, it is characterized in that comprising the component of following percentage by weight:
Above-mentioned Chinese medicine extract can adopt solvent extraction method to obtain, and after for example above-mentioned Chinese medicine can being pulverized, sieves, soak or heating extraction with extracting solvent, extracting solution is used macroporous resin adsorption after centrifugalize, ethanol with 10～95% is eluting repeatedly, collects eluent, obtains behind the concentrating under reduced pressure.For fear of dissolvent residual, the extraction solvent that uses among the present invention can adopt water, ethanol, ethanol water.
The application of above-mentioned Chinese medicine extraction compositions in the external preparations such as preparation U.S. black cosmetics, black hair cosmetics and therapy of vitiligo product, it is characterized in that containing in these external preparations above-mentioned Chinese medicine extraction compositions, its consumption is 0.05～25%, and preferable amount is 0.5～10%.Except containing above-mentioned Chinese medicine extraction compositions, can suitably add as required the common composition that is fit to the skin preparations for extenal use use, such as matrix components such as wetting agent, emollient, water, emulsifying agent, surfactants.
The dosage form of the external preparations such as U.S. black cosmetics, black hair cosmetics and therapy of vitiligo product is not particularly limited, and can be solution, emulsion, paste, powdery, foundation emulsion, spray etc.
Beneficial effect of the present invention: Chinese medicine extraction compositions of the present invention has the effect that activates melanocyte, activates tryrosinase, can generate by the establishment dermal melanin, has the effect of excellent melanin formation promoting.Said composition does not contain the material of chemosynthesis, has no side effect, and skin irritation is little, and stable in properties is safe and reliable.In addition, except promoting melanin to generate the effect, because said composition contains the plurality of Chinese active component, function with activating blood circulation to dissipate blood stasis, can be skin and directly provide and nourish nutrition composition in its normal physiological processes, promote skin metabolism, improve the health of skin, have good skin effect.
The specific embodiment
Below come by specific embodiment that the present invention is further illustrated, but these embodiment are not limitations of the present invention just in order to illustrate.
Embodiment 1 melanin generates facilitation effect evaluation experimental method
1, the preparation of sample
Above-mentioned Chinese medicine extraction compositions is dissolved in the dimethyl sulfoxine, is made into concentration and is 1% sample solution, carry out following test.
2, activate the test of tryrosinase vigor effect assessment
Accurately drawing sample solution, pH value by table 2 is that 6.8 phosphate buffered solution (PBS) and mass fraction are 0.1% DOPA solution, fully mix, in 30 ℃ hatch 5min after, adding activity is the tryrosinase solution of 110U/mL, under 30 ℃, hatch 10min again, be transferred to rapidly in the cuvette, survey absorbance at the 475nm place.Use in contrast thing of kojic acid, hydroquinone, ascorbic acid, arbutin.
Table 2
Be calculated as follows sample to the relative activity ratio (A is called for short activity ratio, and is lower same) of tryrosinase.
In the formula: OD
1, OD
2, OD
3, OD
4Be respectively the absorbance of 1 group～4 groups of solution.
3, activate melanin synthetic test in the melanocyte
The density of B16 melanoma cell with 1 * 105/mL is inoculated in 96 orifice plates, every hole 90 μ L, hatch 24 hours in the CO2 incubator after, every hole adds sample solution.Set up simultaneously the blank group of culture medium and cell.
After placing incubator to hatch 72 hours the culture plate, abandoning supernatant, PBS washing twice, then every hole adds 0.5mL trypsin digestion cell 3min, and every hole adds the 2mL maintenance medium and stops digestion.Behind the mixing, every kind of concentration is taken out 0.5mL and is done the cell counting.All the other cell suspension are with 2500r/min centrifugalize 5min, and abandoning supernatant adds NaOH solution in precipitation, and heating makes the melanin dissolving, selects the 490nm wavelength to measure absorbance under enzyme-linked immunosorbent assay instrument.Be calculated as follows sample to the synthetic activity ratio of melanin (A ').
In the formula: OD
5Be medicine hole absorbance; P
1Be medicine porocyte density; OD
6Be the control wells absorbance; P
2Be the control wells cell density.
The preparation of embodiment 2 Chinese medicine extract
1, the preparation of Rhizoma Gastrodiae extract
After Rhizoma Gastrodiae 1kg pulverizes, cross 40 mesh sieves, add 70% ethanol 6kg, be heated to 60 ℃ and extract 2h, repeat to extract 2 times, extracting solution is used macroporous resin adsorption after centrifugalize, and the ethanol with 95% is eluting repeatedly, collect eluent, be evaporated to 200g after, obtain Rhizoma Gastrodiae extract.
2, the preparation of Fructus Luffae (fresh) extract
After Fructus Luffae (fresh) 1kg pulverizes, add 10% ethanol 3kg, be heated to 50 ℃ and extract 2h, repeat to extract 2 times, extracting solution is used macroporous resin adsorption after centrifugalize, ethanol with 30% is eluting repeatedly, collect eluent, be evaporated to 200g after, obtain Fructus Luffae (fresh) extract.
3, the preparation of Semen Phaseoli extract
After Semen Phaseoli 1kg pulverizes, cross 60 mesh sieves, add deionized water 8kg, be heated to 60 ℃ and extract 3h, repeat to extract 2 times, extracting solution is used macroporous resin adsorption after centrifugalize, and the ethanol with 20% is eluting repeatedly, collect eluent, be evaporated to 200g after, obtain the Semen Phaseoli extract.
4, the preparation of Spica Prunellae extract
After Spica Prunellae 1kg pulverizes, cross 60 mesh sieves, add 50% ethanol 7kg, heating and refluxing extraction 2h, repeat to extract 2 times, extracting solution is used macroporous resin adsorption after centrifugalize, and the ethanol with 75% is eluting repeatedly, collect eluent, be evaporated to 200g after, obtain Spica Prunellae extract.
5, the preparation of Semen Raphani extract
After Semen Raphani 1kg pulverizes, cross 60 mesh sieves, add 70% ethanol 6kg, be heated to 70 ℃ and extract 2.5h, repeat to extract 2 times, extracting solution is used macroporous resin adsorption after centrifugalize, and the ethanol with 95% is eluting repeatedly, collect eluent, be evaporated to 200g after, obtain Semen Raphani extract.
Embodiment 3 Chinese medicine extraction compositions (I)
Have and promote that the Chinese medicine extraction compositions (I) of melanin systematic function is composed as follows by weight percentage:
1% sample solution of said composition is 89.1% to the activity ratio of tryrosinase, is 77.6% to the synthetic activity ratio of melanin
Embodiment 4 Chinese medicine extraction compositions (II)
Have and promote that the Chinese medicine extraction compositions (II) of melanin systematic function is composed as follows by weight percentage:
1% sample solution of said composition is 87.6% to the activity ratio of tryrosinase, is 75.1% to the synthetic activity ratio of melanin
Embodiment 5 Chinese medicine extraction compositions (III)
Have and promote that the Chinese medicine extraction compositions (III) of melanin systematic function is composed as follows by weight percentage:
1% sample solution of said composition is 91.2% to the activity ratio of tryrosinase, is 80.7% to the synthetic activity ratio of melanin
Embodiment 6 therapy of vitiligo frosts and preparation method thereof
Table 4 provides the U.S. black frost prescription of the skin that contains the Chinese medicine extraction compositions, and the consumption of each material represents with mass fraction.
Table 4
The preparation method of therapy of vitiligo frost: the white mineral oil in the formula table, dimethyl siloxane, isopropyl palmitate, Miglyol 812, monoglyceride, stearic acid, methyl hydroxybenzoate, propylparaben are mixed, be heated to 80 ℃, fusing stirs, as oil phase; 1,3 butylene glycol in the formula table, Chinese medicine extraction compositions, potassium hydroxide, deionized water are mixed, be heated to 85 ℃, stirring and dissolving is even, as water.Oil phase and water are added in the emulsifying pot, stir, emulsifying homogeneous stirred when being cooled to 50 ℃ after 5 minutes, added essence, stirred, and was cooled to 38 ℃, was the therapy of vitiligo frost.
Embodiment 7 U.S. black facial treatment essence and preparation method thereof
Table 5 provides the black facial treatment essence prescription of U.S. of containing the Chinese medicine extraction compositions, and the consumption of each material represents with mass fraction.
Table 5
The preparation method of U.S. black facial treatment essence: deionized water is heated to 85 ℃, adds hydroxyethyl-cellulose, and stirring and dissolving is even, adds 1,3 butylene glycol.When stirring is cooled to 50 ℃, add other compositions, continue stirring and be cooled to 38 ℃, be U.S. black facial treatment essence.
Embodiment 8 black hair shampoos and preparation method thereof
Table 6 provides the black hair shampoo formula that contains the Chinese medicine extraction compositions, and the consumption of each material represents with mass fraction.
Table 6
Black hair shampoo preparation method: deionized water is heated to 80 ℃, adds cation guar gum, after the insulation dissolving evenly, add disodiumedetate, citric acid, sodium chloride, stirring and dissolving.Then, add the Dodecyl Polyoxyethylene Ether sodium sulfovinate, stirring and dissolving.Add stearic acid diethylene glycol dilaurate, cetostearyl alcohol, stirring and dissolving.Add lauric acid amide of ethanol, cocamido propyl betaine and Chinese medicine extraction compositions, stir, pass into water quench to 50 ℃, add KF88 antiseptic, emulsified silicone oil and essence, when continuing to be cooled to 40 ℃, be the black hair shampoo.
Embodiment 9 black hair hair conditioner and preparation method thereof
Table 7 provides the black hair hair conditioner prescription that contains the Chinese medicine extraction compositions, and the consumption of each material represents with mass fraction.
Table 7
Black hair hair conditioner preparation method: INCROQUAT TMC-80 ECONOL TM22, disodiumedetate are added in the deionized water, are heated to 80 ℃, be water.Glyceryl monostearate, cetostearyl alcohol, polydimethylsiloxane, amino silicone are mixed, be heated to 80 ℃, be oil phase.Oil phase and water are added in the emulsifying pot, stir, emulsifying homogeneous stirred when being cooled to 50 ℃ after 5 minutes, added essence, KF88 antiseptic and Chinese medicine extraction compositions, stirred, and was cooled to 38 ℃, was black hair hair conditioner.
Claims (4)
1. one kind has the Chinese medicine extraction compositions that promotes the melanin systematic function, it is characterized in that by weight percentage, composed of the following components:
Rhizoma Gastrodiae extract 2～30%,
Fructus Luffae (fresh) extract 2～30%,
Semen Phaseoli extract 2～30%,
Spica Prunellae extract 2～30%,
Semen Raphani extract 2～30%;
Above-mentioned Chinese medicine extract adopts solvent extraction method to obtain: after above-mentioned Chinese medicine is pulverized, sieve, soak or heating extraction with extracting solvent, extracting solution is used macroporous resin adsorption after centrifugalize, use repeatedly eluting of 10～95% ethanol again, collect eluent, obtain behind the concentrating under reduced pressure; Described extraction solvent is water, ethanol, ethanol water.
2. the application of Chinese medicine extraction compositions claimed in claim 1 on the U.S. black cosmetics of preparation skin.
3. the application of Chinese medicine extraction compositions claimed in claim 1 on the leukodermic external preparation for skin medicine of preparation treatment.
4. the application of Chinese medicine extraction compositions claimed in claim 1 on preparation black hair cosmetics.
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| CN101288738A (en) * | 2008-06-19 | 2008-10-22 | 魏永全 | Production method of medicine for treating vitiligo |
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| CN101288738A (en) * | 2008-06-19 | 2008-10-22 | 魏永全 | Production method of medicine for treating vitiligo |
Non-Patent Citations (4)
| Title |
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| 浅议黑豆美容的古今应用;陈少丽等;《辽宁中医学院学报》;20050918(第05期);503-504 * |
| 白癜风制剂配合生发制剂治疗少年白发36例;陈思宏等;《陕西中医》;20070405(第04期);450-451 * |
| 陈少丽等.浅议黑豆美容的古今应用.《辽宁中医学院学报》.2005,(第05期), |
| 陈思宏等.白癜风制剂配合生发制剂治疗少年白发36例.《陕西中医》.2007,(第04期), |
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Inventor after: Gong Shengzhao Inventor after: Huang Cairong Inventor before: Gong Shengzhao |
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Effective date of registration: 20160323 Address after: 510300 Xingang West Road, Guangdong, Guangzhou, No. 152, No. Patentee after: Guangdong Industry Technical College Patentee after: GUANGDONG GADETIN DAILY NECESSITIES CO., LTD. Address before: 510300 Xingang West Road, Guangdong, Guangzhou, No. 152, No. Patentee before: Guangdong Industry Technical College |