CN102428885B - Family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle - Google Patents

Family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle Download PDF

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Publication number
CN102428885B
CN102428885B CN2011102831858A CN201110283185A CN102428885B CN 102428885 B CN102428885 B CN 102428885B CN 2011102831858 A CN2011102831858 A CN 2011102831858A CN 201110283185 A CN201110283185 A CN 201110283185A CN 102428885 B CN102428885 B CN 102428885B
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shelled turtle
soft
family
turtle
mark
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CN102428885A (en
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许晓军
张海琪
杜建明
何中央
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ZHEJIANG PROVINCE AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION
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ZHEJIANG PROVINCE AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

A family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle is provided, belonging to the aquaculture technical field, comprising the steps of marking individual of each family young turtle which is just batched, injecting a marking liquid mixed by fluorescence labeling colloid marker and curing agent according to the proportion of 10: 1; inserting needle from ventral faces of four legs of the young turtle and then inserting to the gap of the skin and muscle along a body direction from end of the leg; lightly pushing the injector to slowly draw theneedle head outwards in order to form a spot mark; after marking and injecting, use the wet cloth to erase marking liquid overflowing the needle hole. The method has good marking effect, has high mark retention rate and identification effect and does not damage the young turtle; the growth rate and survival rate of the turtle are not influenced by injecting mark by comparison of the marked group and non-marked group; the problems that the Chinese softshell turtle cannot be bred in the same pond without family individual marking during the traditional improved variety breeding and the estimation accuracy of the breeding production performance are changed; the estimation accuracy of improved variety breeding of Chinese softshell turtle is improved effectively.

Description

The method of family individual fluorescence labeling in China's soft-shelled turtle fine-variety breeding
Technical field
The invention belongs to technical field of aquaculture, be specifically related to the method for family individual fluorescence labeling in a kind of Chinese soft-shelled turtle fine-variety breeding.
Background technology
The China soft-shelled turtle is one of important famous-particular-excellent aquaculture kind of China, and its aquaculture is China's developing rural economy, the high-quality and efficient industry that improves farmers' income always, has therefore become industry and the developing direction of giving special assistance to all parts of the country.Along with the fast development of supporting the soft-shelled turtle industry, the research of breeding technique lags behind the development of cultural technique, occur in succession that poor growth, forming specification are little, degradation phenomenon under sick popular, the quality of soft-shelled turtle, restricting the healthy sustainable development of China China soft-shelled turtle aquaculture, so the utmost point is necessary to carry out Chinese soft-shelled turtle fine-variety breeding.And the breed Performance Evaluation of separate sources family is most important in the fine-variety breeding process, and its result determines the direction of fine-variety breeding from now on.
In traditional fine-variety breeding process, mainly utilize different ponds under the breeding way of the same terms as far as possible, to carry out the breeding production Performance Evaluation.This method is existence condition difference more or less, needs to use a large amount of ponds, and is strict to experimental design, and accuracy is not high as a result.
Since Zhejiang Province aquatic products technology popularization master station controls the fine-variety breeding of inbreeding depression since Chinese soft-shelled turtle in 2003, exploring the individual marking method of the Chinese soft-shelled turtle of different familys always, how to realize that the same pond of different Chinese soft-shelled turtle familys raises together with, and do not need too many pond just can culture Performance Evaluation to a large amount of familys to become the technical barrier that needs to be resolved hurrily.
Summary of the invention
At the above-mentioned problems in the prior art, the object of the present invention is to provide the method for family individual fluorescence labeling in a kind of Chinese soft-shelled turtle fine-variety breeding, having solved in the existing Chinese soft-shelled turtle fine-variety breeding process different familys can't raise together with the pond, because of problem such as the breed Performance Evaluation accuracy of carrying out under the different pond conditions is not high, realize the individual fluorescence labeling of different Chinese soft-shelled turtle familys, can carry out the breed Performance Evaluation with the pond.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding is characterized in that described fluorescence labeling method comprises the steps:
1) young soft-shelled turtle is originated: the spawning ground from the different close soft-shelled turtles of Chinese soft-shelled turtle pond takes the soft-shelled turtle ovum earlier, after each nest ovum carried out surveying record, record weight and the quantity of ovum in every nest, putting into the hatching basket hatches synchronously, the young soft-shelled turtle of each family after the hatching was deposited respectively 1 day with the little basin of plastics, the young soft-shelled turtle of each family is carried out surveying record in order to mark, record weight and the quantity of young soft-shelled turtle;
2) preparation of marking fluid: red, orange, blue, green four look fluorescence labeling colloid labels are fully mixed for the ratio of 10:1 by volume with curing agent respectively, it is standby to obtain marking fluid, and described fluorescence labeling colloid and curing agent are the VIE fluorescence labeling by U.S. northwest marine thechnology company;
3) injection of marking fluid: get step 2 with the 0.3mL syringe by actual needs) marking fluid of the different colours that obtains is injected the young soft-shelled turtle that step 1) obtains, the young soft-shelled turtle four limbs outside of belly inserting needle that syringe needle obtains from step 1), insert skin and muscle gap along acra to the trunk direction, the limit is touched the syringe limit and slowly syringe needle is outwards extracted out to form mottled mark during injection of labelled, the marking fluid that overflow at the pin hole place is wiped with wet cloth in mark injection back, and described spot is that diameter is circle or the ellipsoidal structure of 2mm-3mm.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding is characterized in that the young soft-shelled turtle body weight described in the step 1) greater than 3g, and labelled amount is greater than 5 μ L.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding, it is characterized in that described in the step 1) to put into the hatching basket temperature of hatching synchronously be 29 ℃-31 ℃, temperature also can be finely tuned up and down according to actual needs.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding, when it is characterized in that the young soft-shelled turtle body weight described in the step 1) is 3.0g-5.0g, labelled amount is 5 μ L-10 μ L.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding is characterized in that the storage temperature of depositing 1 day with the little basin of plastics described in the step 1) is 29 ℃-31 ℃.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding is characterized in that described every the young soft-shelled turtle of step 3) can inject one or more marking fluid.
The method of family individual fluorescence labeling in the described Chinese soft-shelled turtle fine-variety breeding is characterized in that the described mark position of step 3) is that young soft-shelled turtle four limbs outside of belly skin is thinner, the zone of no melanin pigmentation.
By adopting above-mentioned technology, compared with prior art, the present invention has following beneficial effect:
1) the present invention is simple to operate, and identification is convenient, applied range;
2) the present invention is applicable to that firm hatching back body weight is the individual mark of the young soft-shelled turtle of 3.0g-5.0g family, injection of labelled does not cause damage to young soft-shelled turtle, breed by mark group and unmarked group is relatively injected 1-2 mark and can growth rate and the survival rate of soft-shelled turtle class not exerted an influence;
3) mark of the present invention is effective, has high mark conservation rate and recognition effect;
4) but the present invention chooses 4 1-2 in the mark position carries out mark, in conjunction with 112 kinds of different types of the mark of 4 kinds of different colours one-tenth capable of being combined.
Embodiment
The invention will be further described below in conjunction with embodiment, but protection scope of the present invention is not limited in this:
But the present invention chooses 4 1-2 in the mark position carries out mark, in conjunction with 112 kinds of different types of the mark of 4 kinds of different colours one-tenth capable of being combined, as shown in table 3.
Embodiment 1
Implement in August, 2010 in the provincial Chinese soft-shelled turtle seed multiplication farm of Zhejiang Province's Xianoshan City.
The method of family individual fluorescence labeling may further comprise the steps in China's soft-shelled turtle fine-variety breeding:
1) young soft-shelled turtle is originated: the spawning ground in China soft-shelled turtle parent soft-shelled turtle pond takes soft-shelled turtle ovum 74 nests from 3 ages, amounts to 1112 pieces.It is in 29 ℃-31 ℃ the little plastics hatching basket that each nest soft-shelled turtle ovum is placed on temperature separately, hatch synchronously after the numbering, after the young soft-shelled turtle hatching, it is to put 1 day in 29 ℃ of-31 ℃ of little plastic basin that the young soft-shelled turtle of each family is placed on temperature separately, finally obtain 69 of young soft-shelled turtle familys, amount to 1022.
2) tagging scheme: adopt red, orange, blue, green four look fluorescence labels to carry out the family mark.69 Chinese soft-shelled turtle familys are pressed 1 to No. 69 mode mark in the table 3.
3) after fully being mixed in the ratio of 10:1, coloured fluorescence labeling colloid and curing agent be transferred to the 0.3mL syringe.Syringe needle is from the four limbs outside of belly inserting needle during mark, insert skin and muscle gap along acra to the trunk direction, the limit is touched the syringe limit and slowly syringe needle is outwards extracted out form the circular or oval-shaped mottled mark that diameter is 2mm-3mm during injection of labelled, and the mark that overflow at the pin hole place is wiped with wet cloth in mark injection back.Mark position is that four limbs outside of belly skin is thinner, the zone of no melanin pigmentation, and every young soft-shelled turtle labelled amount is 5 μ L-10 μ L.
Altogether mark 1022 individualities, the young soft-shelled turtle that mark is good was supported 1 day in the little basin of plastics temporarily, put into Chinese soft-shelled turtle family again and carried out raising together with the pond, selected 500 unmarked young soft-shelled turtles else and compared with control group and compare, results of comparison is as shown in table 1.As can be seen from Table 1, the culture experiment through 2 months, mark group body weight specification reaches 54.2g, and survival rate reaches 99.6%, does not have significant difference with control group.The mark storage rate reaches 99.2%, has realized that the Chinese soft-shelled turtle of different familys is with pool cultivated Performance Evaluation.
The unmarked young soft-shelled turtle of table 1 and the young soft-shelled turtle of control group are with the pool cultivated table of comparisons as a result
? The body weight specification Survival rate % Mark storage rate %
Family through mark 54.2g 99.6 99.2
The family contrast of un-marked 55.0g 99.4 /
Embodiment 2
In day provincial Chinese soft-shelled turtle seed multiplication farm of sea, XiaoShan, HangZhou City, Zhejiang Province, implemented in 2010.
Implement in August, 2010 in the provincial Chinese soft-shelled turtle seed multiplication farm of Zhejiang Province's Xianoshan City.
The method of family individual fluorescence labeling may further comprise the steps in China's soft-shelled turtle fine-variety breeding:
1), young soft-shelled turtle source: the spawning ground in China soft-shelled turtle parent soft-shelled turtle pond takes soft-shelled turtle ovum 51 nests from 3 ages, amounts to 526 pieces.Each nest soft-shelled turtle ovum is placed on separately in the little plastics hatching basket, hatches synchronously after the numbering.After the young soft-shelled turtle hatching, young soft-shelled turtle is placed on separately in the little plastic basin each family.Finally obtain 48 of young soft-shelled turtle familys, amount to 486.
2), tagging scheme: adopt red, orange, blue, green four look fluorescence labels to carry out the family mark.Each Chinese soft-shelled turtle family mark, with different colors, same soft-shelled turtle can be with the different position of identical or different color mark, and be as shown in table 3 at different positions.
Be transferred to the 0.3mL syringe after coloured fluorescence labeling colloid and curing agent fully mixed in the ratio of 10:1.Syringe needle is from the four limbs outside of belly inserting needle during mark, insert skin and muscle gap along acra to the trunk direction, the limit is touched the syringe limit and is slowly outwards extracted syringe needle out to form mottled mark during injection of labelled, and the mark that overflow at the pin hole place is wiped with wet cloth in mark injection back.Mark position is that four limbs outside of belly skin is thinner, the zone of no melanin pigmentation, and every young soft-shelled turtle labelled amount is 5 μ L-10 μ L.
Altogether mark 486 individualities, the young soft-shelled turtle that mark is good was supported 1 day in the little basin of plastics temporarily, put into Chinese soft-shelled turtle family again and carried out raising together with the pond, selected 500 unmarked young soft-shelled turtles else and compared with control group and compare, results of comparison is as shown in table 2.Compare with control group, the culture experiment through 2 months, mark group body weight specification reaches 52.6 g, and survival rate reaches 99.0%, does not have significant difference with control group.The mark storage rate reaches 100%, has realized that the Chinese soft-shelled turtle of different familys is with pool cultivated Performance Evaluation.
The unmarked young soft-shelled turtle of table 2 and the young soft-shelled turtle of control group are with the pool cultivated table of comparisons as a result
? The body weight specification Survival rate % Mark storage rate %
Family through mark 52.6g 99.0 100
The family contrast of un-marked 52.0g 99.2 /
The Chinese soft-shelled turtle fluorescence labeling of table 3 family numbering and the mark mode table of comparisons
Sequence number Mark mode Sequence number Mark mode Sequence number Mark mode Sequence number Mark mode
1 Left front red 29 The right front orange of left front orange 57 Left front indigo plant is left back green 85 The left back orange of right front indigo plant
2 Right front red 30 The left back orange of left front orange 58 Left front indigo plant is right back green 86 The right back orange of right front indigo plant
3 Left back red 31 The right back orange of left front orange 59 Left front orange is right front red 87 Right front indigo plant is left back green
4 Right back red 32 The left back orange of right front orange 60 Left front orange is left back red 88 Right front indigo plant is right back green
5 Left front indigo plant 33 The right back orange of right front orange 61 Left front orange is right back red 89 Right front orange is left back red
6 Right front indigo plant 34 The right back orange of left back orange 62 The right front indigo plant of left front orange 90 Right front orange is right back red
7 Left back indigo plant 35 Left front green right front green 63 The left back indigo plant of left front orange 91 The left back indigo plant of right front orange
8 Right back indigo plant 36 Left front green left back green 64 The right back indigo plant of left front orange 92 The right back indigo plant of right front orange
9 Left front orange 37 Left front green right back green 65 Left front orange is right front green 93 Right front orange is left back green
10 Right front orange 38 Right front green left back green 66 Left front orange is left back green 94 Right front orange is right back green
11 Left back orange 39 Right front green right back green 67 Left front orange is right back green 95 Right front green left back red
12 Right back orange 40 Left back green right back green 68 Left front green right front red 96 Right front green right back red
13 Left front green 41 Left front red right front indigo plant 69 Left front green left back red 97 Right front green left back indigo plant
14 Right front green 42 Left front red left back indigo plant 70 Left front green right back red 98 Right front green right back indigo plant
15 Left back green 43 Left front red right back indigo plant 71 Left front green right front indigo plant 99 Right front green left back orange
16 Right back green 44 Left front red right front orange 72 Left front green left back indigo plant 100 Right front green right back orange
17 Left front red right front red 45 Left front red left back orange 73 Left front green right back indigo plant 101 Left back red right back indigo plant
18 Left front red left back red 46 Left front red right back orange 74 Left front green right front orange 102 Left back red right back orange
19 Left front red right back red 47 Left front red right front green 75 Left front green left back orange 103 Left back red right back green
20 Right front red left back red 48 Left front red left back green 76 Left front green right back orange 104 Left back indigo plant is right back red
21 Right front red right back red 49 Left front red right back green 77 Right front red left back indigo plant 105 The right back orange of left back indigo plant
22 Left back red right back red 50 Left front indigo plant is right front red 78 Right front red right back indigo plant 106 Left back indigo plant is right back green
23 The right front indigo plant of left front indigo plant 51 Left front indigo plant is left back red 79 Right front red left back orange 107 Left back orange is right back red
24 The left back indigo plant of left front indigo plant 52 Left front indigo plant is right back red 80 Right front red right back orange 108 The right back indigo plant of left back orange
25 The right back indigo plant of left front indigo plant 53 The right front orange of left front indigo plant 81 Right front red left back green 109 Left back orange is right back green
26 The left back indigo plant of right front indigo plant 54 The left back orange of left front indigo plant 82 Right front red right back green 110 Left back green right back red
27 The right back indigo plant of right front indigo plant 55 The right back orange of left front indigo plant 83 Right front indigo plant is left back red 111 Left back green right back indigo plant
28 The right back indigo plant of left back indigo plant 56 Left front indigo plant is right front green 84 Right front indigo plant is right back red 112 Left back green right back orange

Claims (7)

1. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding is characterized in that described fluorescence labeling method comprises the steps:
1) young soft-shelled turtle is originated: the spawning ground from the different close soft-shelled turtles of Chinese soft-shelled turtle pond takes the soft-shelled turtle ovum earlier, after each nest ovum carried out surveying record, putting into the hatching basket hatches synchronously, it is to support 1 day temporarily in 29 ℃ of-31 ℃ of little plastic basin that the young soft-shelled turtle of each family after the hatching is placed on temperature separately, the young soft-shelled turtle of each family is carried out surveying record in order to mark, and labelled amount is 5 μ L-10 μ L;
2) preparation of marking fluid: with red, orange, blue, green four look fluorescence labeling colloid labels respectively with curing agent by volume for the ratio of 10:1 fully mixes, it is standby to obtain marking fluid;
3) injection of marking fluid: get step 2 with the 0.3mL syringe by actual needs) marking fluid of the different colours that obtains is injected the young soft-shelled turtle that step 1) obtains, the young soft-shelled turtle four limbs outside of belly inserting needle that syringe needle obtains from step 1), insert skin and muscle gap along acra to the trunk direction, the limit is touched the syringe limit and is slowly outwards extracted syringe needle out to form mottled mark during injection of labelled, and the marking fluid that overflow at the pin hole place is wiped with wet cloth in mark injection back.
2. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1 is characterized in that the young soft-shelled turtle body weight described in the step 1) greater than 3g, and labelled amount is greater than 5 μ L.
3. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1, it is characterized in that described in the step 1) to put into the temperature that the hatching basket hatches synchronously be 29 ℃-31 ℃.
4. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1 is when is characterized in that the young soft-shelled turtle body weight described in the step 1) is 3.0g-5.0g.
5. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1 is characterized in that the storage temperature of depositing 1 day with the little basin of plastics described in the step 1) is 29 ℃-31 ℃.
6. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1 is characterized in that described every the young soft-shelled turtle of step 3) can inject one or more marking fluid.
7. the method for family individual fluorescence labeling in the Chinese soft-shelled turtle fine-variety breeding according to claim 1 is characterized in that the described mark position of step 3) is that young soft-shelled turtle four limbs outside of belly skin is thinner, the zone of no melanin pigmentation.
CN2011102831858A 2011-09-19 2011-09-22 Family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle Expired - Fee Related CN102428885B (en)

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104663554A (en) * 2015-03-26 2015-06-03 梧州市景泰养鳖专业合作社 Selection method of Chinese softshell turtle parent turtles
CN105918174B (en) * 2016-05-03 2019-03-05 华中农业大学 Family individual fluorescence labeling method in a kind of loach fine-variety breeding
CN112868585B (en) * 2021-01-26 2023-01-03 浙江省淡水水产研究所 Method suitable for large-scale breeding individual marking of red crayfish families

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CN101120662A (en) * 2007-08-04 2008-02-13 杭州萧山天福生物科技有限公司 Method for selectively breeding improved variety of China turtle
CN101433188A (en) * 2008-12-10 2009-05-20 中国水产科学研究院黄海水产研究所 Individual marking method for selective breeding of Tilapia
CN101911921A (en) * 2010-09-16 2010-12-15 厦门大学 Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs
CN102165924A (en) * 2011-01-20 2011-08-31 中国科学院南海海洋研究所 Fluorescence labeling method for sea cucumber larvas

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120662A (en) * 2007-08-04 2008-02-13 杭州萧山天福生物科技有限公司 Method for selectively breeding improved variety of China turtle
CN101433188A (en) * 2008-12-10 2009-05-20 中国水产科学研究院黄海水产研究所 Individual marking method for selective breeding of Tilapia
CN101911921A (en) * 2010-09-16 2010-12-15 厦门大学 Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs
CN102165924A (en) * 2011-01-20 2011-08-31 中国科学院南海海洋研究所 Fluorescence labeling method for sea cucumber larvas

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