CN102599087B - Fluorescence labeling method for adult mud crab - Google Patents
Fluorescence labeling method for adult mud crab Download PDFInfo
- Publication number
- CN102599087B CN102599087B CN201210058510.5A CN201210058510A CN102599087B CN 102599087 B CN102599087 B CN 102599087B CN 201210058510 A CN201210058510 A CN 201210058510A CN 102599087 B CN102599087 B CN 102599087B
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- China
- Prior art keywords
- crab
- mud
- mud crab
- labeling method
- fluorescence labeling
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A fluorescence labeling method for adult mud crab comprises (1) preparing fluorescent gel label and filling into sterile syringe; (2) inserting syringe needle along joint middle seam protrusion of natatorial leg of mud crab, drawing out syringe needle while injecting, to form uniform strip-shaped injected fluorescent gel; and (3) wiping off residual gel at needle insertion site with sterilized cotton. The invention has simple operation, freely adjustable fluorescent gel color, no damage on crab, no influence on growth and behavior, and suitability for other crabs with natatorial leg.
Description
Technical field
The invention belongs to fluorescence labeling field, particularly a kind of mud crab becomes crab fluorescence labeling method.
Background technology
In marine fishery resources work and aquaculture work, the individuality that usually need individuality to enhancement releasing, carries out hereditary and selection carries out mark, has developed at present multiple aquatic products species labeling method.But the mark of crab class is a difficult problem always, because its surface is covered by crust, and repeatedly shell in process of growth, amputation meeting is regrowed after shell, causes most labeling methods all cannot be applied to crab class.Comprehensively relatively get off, fluorescent colloid labeling technique is simple to operate, little to biological damage, and cost is low, but must overcome crab class, has crust, and the difficult point of meeting shell in process of growth, so still there is not gratifying fluorescence labeling method always.Davis was once injected at fluorescent colloid label in the segmental appendage of pedes natatorii of blue crab children crab, once but blue crab grow up, after pedes natatorii crust thickening effect with regard to very poor (Fisheries Research, 2004,67:265-274).Spilseth is expelled to by fluorescent colloid the coxopodite of appendage and the transparent joint (Crustaceana of base pitch that the thick line crab of big legs becomes crab, 2005,78:1383-1388), but this labeling method must catch each crab respectively while observing, and stand up and look round, when being in culturing pool, crab because can only see the carapace back side, cannot observe directly mark position.Wang Guizhong etc. are injected at fluorescent colloid mark the bound fraction (national inventing patent application 201010283551.5) of mud crab children's crab cephalothorax outside of belly cortex and muscle, but once young crab grows up and becomes into crab, because crust thickening is difficult to observe fluorescence labeling, because normal condition crab be all the outside of belly down, during observation, to see belly, crab must be caught up or crab is turned.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of mud crab and becomes crab fluorescence labeling method, and the method is simple to operate, and the color of fluorescent colloid can freely be allocated, and to crab class not damaged, on growth and movable without impact, is also applicable to the crab class that other have pedes natatorii.
A kind of mud crab of the present invention becomes crab fluorescence labeling method, comprising:
(1) preparation fluorescent colloid label, and fluorescent colloid label is injected in asepsis injector stand-by;
(2) while injecting fluorescence labels, syringe needle is inserted along the middle seam prominence of mud crab pedes natatorii dactylus, syringe needle is outwards extracted out, limit is taken out marginal not and is penetrated, make the fluorescent colloid of injection be uniform strip, after syringe needle is extracted out, with disinfecting silk or cotton, wipe syringe insert division residual colloid around.
The color of the fluorescent colloid label in described step (1) is one or more in red, yellow, green, orange, can distinguish with mud crab pedes natatorii body colour.
Mud crab in described step (2) is for becoming crab.
The rectangular length of fluorescence labels in described step (2) is middle seam prominence length over half of mud crab pedes natatorii dactylus.
beneficial effect
1) the present invention can be applicable to the mark that mud crab becomes crab, although become the crust thickening of crab to become opaque, the middle seam prominence crust of pedes natatorii dactylus is very thin and transparent, can clearly observe the fluorescent colloid of injection;
2) fluorescent colloid of the present invention's injection can not lost with mud crab shell;
3) fluorescent colloid of mark of the present invention, mud crab nature in culturing pool just can be observed, without crab body is turned over or caught;
4) the present invention is simple to operate, and the color of fluorescent colloid can freely be allocated, and to crab class not damaged, on growth and movable without impact, is also applicable to the crab class that other have pedes natatorii.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
1) preparation red fluorescence colloid label, and fluorescent colloid label is injected in asepsis injector stand-by;
2) while injecting fluorescence labels, syringe needle is inserted along the middle seam projection of mud crab pedes natatorii dactylus, syringe needle is outwards slowly extracted out, limit is taken out marginal not and is penetrated, make the fluorescent colloid of injection be uniform strip, and reach middle seam prominence length over half of mud crab pedes natatorii dactylus, after syringe needle is extracted out, with disinfecting silk or cotton, wipe syringe insert division residual colloid around.
Mud crab after injection is put into culturing pool, the fresh foods such as clam of throwing something and feeding.Observe and record lethality and the growth rate of mark mud crab.Same control group, each 15 mud crabs of male and female after 1 month lethality be 0; Growth rate and control group are without significant difference; Pedes natatorii activity performance has no impact.After continuous 2 shells, fluorescent colloid is all without losing, still high-visible.
Claims (4)
1. mud crab becomes a crab fluorescence labeling method, comprising:
(1) preparation fluorescent colloid label, and fluorescent colloid label is injected in asepsis injector stand-by;
(2) while injecting fluorescence labels, syringe needle is inserted along the middle seam prominence of mud crab pedes natatorii dactylus, syringe needle is outwards extracted out, limit is taken out marginal not and is penetrated, make the fluorescent colloid of injection be uniform strip, after syringe needle is extracted out, with disinfecting silk or cotton, wipe syringe insert division residual colloid around.
2. a kind of mud crab according to claim 1 becomes crab fluorescence labeling method, it is characterized in that: the color of the fluorescent colloid label in described step (1) is one or more in red, yellow, green, orange.
3. a kind of mud crab according to claim 1 becomes crab fluorescence labeling method, it is characterized in that: the mud crab in described step (2) is for becoming crab.
4. a kind of mud crab according to claim 1 becomes crab fluorescence labeling method, it is characterized in that: the rectangular length of fluorescence labels in described step (2) is middle seam prominence length over half of mud crab pedes natatorii dactylus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210058510.5A CN102599087B (en) | 2012-03-07 | 2012-03-07 | Fluorescence labeling method for adult mud crab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210058510.5A CN102599087B (en) | 2012-03-07 | 2012-03-07 | Fluorescence labeling method for adult mud crab |
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| Publication Number | Publication Date |
|---|---|
| CN102599087A CN102599087A (en) | 2012-07-25 |
| CN102599087B true CN102599087B (en) | 2014-03-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210058510.5A Expired - Fee Related CN102599087B (en) | 2012-03-07 | 2012-03-07 | Fluorescence labeling method for adult mud crab |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105918174B (en) * | 2016-05-03 | 2019-03-05 | 华中农业大学 | Family individual fluorescence labeling method in a kind of loach fine-variety breeding |
| CN112868585B (en) * | 2021-01-26 | 2023-01-03 | 浙江省淡水水产研究所 | Method suitable for large-scale breeding individual marking of red crayfish families |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731162A (en) * | 2009-12-20 | 2010-06-16 | 浙江海洋学院 | Fluorescent labeling method for inner shell of Sepiella maindroni |
| CN101911921A (en) * | 2010-09-16 | 2010-12-15 | 厦门大学 | Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs |
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2012
- 2012-03-07 CN CN201210058510.5A patent/CN102599087B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731162A (en) * | 2009-12-20 | 2010-06-16 | 浙江海洋学院 | Fluorescent labeling method for inner shell of Sepiella maindroni |
| CN101911921A (en) * | 2010-09-16 | 2010-12-15 | 厦门大学 | Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs |
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| CN102599087A (en) | 2012-07-25 |
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