CN102409013A - Organic material inoculant and preparation method thereof - Google Patents
Organic material inoculant and preparation method thereof Download PDFInfo
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- CN102409013A CN102409013A CN2011103333347A CN201110333334A CN102409013A CN 102409013 A CN102409013 A CN 102409013A CN 2011103333347 A CN2011103333347 A CN 2011103333347A CN 201110333334 A CN201110333334 A CN 201110333334A CN 102409013 A CN102409013 A CN 102409013A
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Abstract
The invention discloses an organic material inoculant, which is a complex microbial community composed of actinomycetes, yeast, lactic acid bacteria, bacillus and photosynthetic bacteria; the effective active bacteria content is 0.5-100000000 unit/g, wherein 40-60% thereof is actinomycetes, the dosage form is powder; the particle diameter of the powder is less than or equal to 0.2 mm, the water content is less than or equal to 20%; the pH value range is 5.5-7.5; and the invention has wine yeast smell. The optimal combination in the complex microbial community is as follows: 50 parts of actinomycetes, 10 parts of yeast, 10 parts of lactic acid bacteria, 15 parts of bacillus and 15 parts of photosynthetic bacteria. The preparation process flow of the material inoculant comprises preparing culturing pot, preparing material, dosing material, loading, culturing and fermenting, drying, crushing, detecting, packaging and storing. The organic material inoculant of the invention is a special organic material inoculant for vinegar residue, cassava residue and other organic materials; the effective active bacteria content is high, the degrading and rotting speed of cellulose is fast. The invention has disadvantages of fast temperature rise, high temperature, strong adaptability and simple preparation; the use effect is good, and the invention is especially suitable for self-culturing and self-consumption in substrate production enterprise.
Description
Technical field
The present invention relates to a kind of microbial fermentation decomposing microbial inoculum, particularly relate to decomposing microbial inoculum of a kind of organic materials and preparation method thereof, belong to the microbial fermentation technology field.
Background technology
Organic wastes such as the acid-sludge of the annual generation of light industries such as China's food, pharmacy, extraction using alcohol enormous amount, the dregs of a decoction, manioc waste; These organic waste utilization ratios are not high; Great majority are all handled as " rubbish ", not only contaminate environment but also cause the wasting of resources.Since some units utilized these organic wastes as the raw material production soilless culture substrate, acid-sludge, the dregs of a decoction, manioc waste etc. became the organic materials resource, yet these organic materialss need just can be used to produce soilless culture substrate through the fermentation maturity processing.Conventional fermentative processing mainly adopts the nature heap fermentation, not only the composting time long, efficient is low, it is inhomogeneous to become thoroughly decomposed, and nutrient runs off in a large number.Chinese patent CN1291006C discloses a kind of microbial starter culture, this starter by aspergillus, mikrobes such as the side spore is mould, the arteries and veins spore is mould, yeast form, and use this starter can improve ferment effect.But this starter is mainly used in organic wastes such as handling agricultural crop straw, feces of livestock and poultry, is not clearly with effects such as its fermentative processing acid-sludge, the dregs of a decoction, manioc wastes.
Summary of the invention
The objective of the invention is to adopt organic wastes such as acid-sludge, manioc waste to the present soilless culture substrate of China is raw material; The present situation that lacks special-purpose decomposing microbial inoculum discloses organic materials specific complex microbiobacterial agent that a kind of temperature is fast, intensification is high, flexibility is strong, effective, making method is simple and easy to do and preparation method thereof.
Technical scheme of the present invention realizes in the following manner:
A kind of organic material composting microbial inoculum is characterized in that: it is the complex microbial community that is formed by combining actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, hundred million units/gram of effective active bacterial content 0.5-1; Wherein actinomycetes account for 40-60%; This microbial inoculum formulation is a pulvis, powder diameter≤0.2mm, water cut≤20%; PH5.5-7.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the said complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is following:
1) prepare to cultivate utensil: prepares long 30-60cm, wide 20-30cm, utensils such as the some and atomizer of the plastic culture dish of 2.5-4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator deeply, prepare 1 of clean culturing room simultaneously; Utensils such as culturing room, vinyl disc, gauze need produced disinfection previous day, and sterilization method can be physical methods such as ultraviolet ray, ozone machine, disinfecting time 5-6 hour; Also can adopt 30-35% formaldehyde solution to add water 7-10 doubly or the chemical disinfection method of 10-20% SANIZOL C, disinfecting time 12-24 hour.
2) get the raw materials ready: prepare rice bran or wheat bran as cultivating bed material; Said rice bran or wheat bran requirement are fresh, dry, nothing is gone mouldy; Fineness≤0.2mm; About water cut 15%, also can use dried acid-sludge powder, cassava ground-slag or straw powder instead of part rice bran or wheat bran, the need that bed material is too thick adopt kibbler to be ground into the particle of fineness≤0.2mm; Make auxiliary material with sugar, vinegar, sugar can be brown sugar, sucrose, glucose, and vinegar can be aromatic vinegar, rice vinegar, mature vinegar etc.
3) batching: press the proportioning of bed material 100kg such as rice bran, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing the 2-5kg rice bran earlier, add water and stir and be adjusted to water cut 38-42%, become bacterium material A with the bacterial classification uniform mixing again; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran and pave on concrete floor or plastic film, take by weighing needed water, stir behind the adding B by the 38-42% water cut; Evenly be sprayed on the rice bran with atomizer, stir, again A is sprinkled into while spraying; Evenly stir, adopt sodium hydroxide or potassium primary phosphate adjustment pH value, form compound C to 5-7; Stacked 1-2 hour, rice bran is fully absorbed water.The calculation formula of amount of water is: the gross weight (kilogram) of answering amount of water=[(matrix requires water cut-rice bran water cut) ÷ (1-matrix requires water cut)] dried rice bran.It is 40 that matrix requires water cut
± 2%.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install C again is " groined type " and is stacked on through in the culturing room that disinfects, height 6-8 layer, and in last, insert TM respectively, indoor extension Hygrothermograph in lower floor's dish.
5) cultivation and fermentation: the cultivation and fermentation management divides three phases, and 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and the material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and the material temperature control is at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, needed this moment the upper lower burrs reversing of position is guaranteed that mycelia evenly grows; This stage must prevent that following three kinds of phenomenons from taking place, the first, and material Wen Taigao in the dish and culturing room's humidity is too low, moisture evaporation is too many, causes the dry hide phenomenon; The second, early stage, mixture moisture was excessive and material temperature is too high or low excessively, the bacterium piece occurred to evacuate phenomenon; The 3rd, the vinyl disc non-uniform charging, charging tray extruding, culturing room are too airtight, expect that perhaps local superheating, moisture are excessive, produce and burn bacterium phenomenon (pale brown look appears in material also has the vinic acid flavor).3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and the material temperature no longer raises and when beginning to descend, in time dispel the heat, and lets the material temperature drop to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter.
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, promptly becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all need detect, and the effective active bacterial content reaches hundred million units/gram of 0.5-1, fineness≤0.2mm, and water cut≤20%, pH5.5-7.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt the plastic opaque bag to pack, every bag of 0.5-2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Beneficial effect of the present invention:
1, organic material composting microbial inoculum of the present invention rise temperature fast, heat up high, flexibility is strong, result of use good.
2, organic material composting microbial inoculum of the present invention is the become thoroughly decomposed special-purpose decomposing microbial inoculum of fermentative prodn soilless culture substrate of organic waste to light industry such as acid-sludges, manioc waste, and its microbe species is many, effective active bacterial content height, and the cellulose degradation speed of becoming thoroughly decomposed is fast.
3, this quick rotting agent making method is simple and easy to do, is particularly suitable for matrix manufacturing enterprise from numerous personal.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment
Below in conjunction with specific embodiment; Further illustrate the present invention; Should understand this embodiment only be used to the present invention is described and be not used in the restriction scope of the present invention; After having read the present invention, those skilled in the art all fall within the application's accompanying claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium, 0.8 hundred million units/gram of effective active bacterial content; Wherein actinomycetes account for about 50%; This microbial inoculum formulation is a pulvis, powder diameter≤0.2mm, water cut≤20%; PH6.0 has the Qu Xiangqi flavor.
The complex microbial community combination is: 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is following:
1) prepare to cultivate utensil: prepares about long 50cm, about wide 30cm, some on the plastic culture dish about 3cm deeply; Reach utensils such as atomizer, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator, prepare 1 of clean culturing room simultaneously.
2) get the raw materials ready: make to cultivate bed material with rice bran, the rice bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water cut about 15%; Make auxiliary material with brown sugar, vinegar; Brown shorts must be pulverized.
3) batching: press the proportioning of rice bran 100kg, brown sugar 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing the 4kg rice bran earlier, add the water stirring and be adjusted to water cut 40 left and right sides %, become bacterium material A with the bacterial classification uniform mixing again; Water with sugar, vinegar adding 4kg is configured to sugar-vinegar liquid B; To remain rice bran and pave on concrete floor or plastic film, take by weighing required water of answering amount of water, stir behind the adding B by 40% left and right sides water cut; Evenly be sprayed on the rice bran with atomizer, stir, again A is sprinkled into while spraying; Evenly stir, adjustment pH value to 6.0 forms compound C; Stacked 2 hours, rice bran is fully absorbed water;
4) sabot: C is packed in the vinyl disc, the thickness 3cm of C around the dish, interior thickness 2.5cm covers moist gauze, guarantees that substratum is exposed; The vinyl disc that will install C again is " groined type " and is stacked on through in the culturing room that disinfects, 7 layers of height, and in the layer dish of upper, middle and lower, insert TM respectively, indoor extension Hygrothermograph;
5) cultivation and fermentation: the cultivation and fermentation management divides three phases, and 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 92 left and right sides %, and the material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and the material temperature control is at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, needed this moment the upper lower burrs reversing of position is guaranteed that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and the material temperature no longer raises and when beginning to descend, in time dispel the heat, and lets the material temperature drop to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter;
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, promptly becomes the organic material composting microbial inoculum after D dries, pulverizes;
7) detect: every batch of microbial inoculum product all need detect, and examination criteria is: 0.8 hundred million units/gram of effective active bacterial content, and fineness≤0.2mm, water cut≤20%, pH6.0 has the Qu Xiangqi flavor;
8) packing, preservation: adopt the plastic opaque bag to pack, every bag of 1kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Embodiment 2:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, 0.5 hundred million units/gram of effective active bacterial content; Wherein actinomycetes account for more than 40%; This microbial inoculum formulation is a pulvis, powder diameter≤0.2mm, water cut≤20%; PH5.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is following:
1) prepare to cultivate utensil: prepares long 30cm, wide 20cm, the some and atomizer of the plastic culture dish of 2.5cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator deeply, prepare 1 of clean culturing room simultaneously.
2) get the raw materials ready: prepare wheat bran as cultivating bed material, the wheat bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water cut about 15%; Make auxiliary material with sugar, vinegar.
3) batching: press the proportioning of wheat bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing the 2-5kg wheat bran earlier, add the water stirring and be adjusted to water cut about 38%, become bacterium material A with the bacterial classification uniform mixing again; The water that sugar, vinegar is added 2kg is configured to sugar-vinegar liquid B; To remain wheat bran and pave on plastic film, take by weighing needed water, stir behind the adding B, evenly be sprayed on the wheat bran with atomizer by 38% water cut; Stir while spraying, again A is sprinkled into, evenly stir, adjustment pH value to 5.0; Form culture medium C, stacked 1 hour, wheat bran is fully absorbed water.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install C again is " groined type " and is stacked on through in the culturing room that disinfects, 6 layers of height, and in last, insert TM respectively, indoor extension Hygrothermograph in lower floor's dish.
5) cultivation and fermentation: the cultivation and fermentation management divides three phases, and 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and the material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and the material temperature control is at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, needed this moment the upper lower burrs reversing of position is guaranteed that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and the material temperature no longer raises and when beginning to descend, in time dispel the heat, and lets the material temperature drop to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter.
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, promptly becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all need detect, and examination criteria is: the effective active bacterial content is not less than 0.5 hundred million units/gram, fineness≤0.2mm, and water cut≤20%, pH5.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt the plastic opaque bag to pack, every bag of 0.5kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Embodiment 3:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, 100,000,000 units/gram of effective active bacterial content; Wherein actinomycetes account for 60%; This microbial inoculum formulation is a pulvis, powder diameter≤0.2mm, water cut≤20%; PH7.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is following:
1) prepare to cultivate utensil: prepares long 60cm, wide 30cm, utensils such as the some and atomizer of the plastic culture dish of 4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator deeply, prepare 1 of clean culturing room simultaneously.
2) get the raw materials ready: as cultivating bed material, the rice bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water cut about 15% with rice bran; Make auxiliary material with sucrose, aromatic vinegar.
3) batching: press the proportioning of rice bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing the 5kg rice bran earlier, add the water stirring and be adjusted to water cut 38-42%, become bacterium material A with the bacterial classification uniform mixing again; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran and pave on concrete floor or plastic film, take by weighing needed water, stir behind the adding B by the 38-42% water cut; Evenly be sprayed on the rice bran with atomizer, stir, again A is sprinkled into while spraying; Evenly stir, adjustment pH value forms the compound culture medium C to 5-7; Stacked 2 hours, rice bran is fully absorbed water.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install C again is " groined type " and is stacked on through in the culturing room that disinfects, 8 layers of height, and in last, insert TM respectively, indoor extension Hygrothermograph in lower floor's dish.
5) cultivation and fermentation: the cultivation and fermentation management divides three phases, and 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and the material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and the material temperature control is at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, needed this moment the upper lower burrs reversing of position is guaranteed that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and the material temperature no longer raises and when beginning to descend, in time dispel the heat, and lets the material temperature drop to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, training in 7-8 days winter.
6) dry, pulverizing: culture medium C obtains mycoplasma D through after the above fermentation culture, promptly becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all need detect, and examination criteria is: 100,000,000 units/gram of effective active bacterial content, and fineness≤0.2mm, water cut≤20%, pH7.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt the plastic opaque bag to pack, every bag of 2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
The agent of becoming thoroughly decomposed that present embodiment is made; It is the become thoroughly decomposed special-purpose decomposing microbial inoculum of fermentative prodn soilless culture substrate of organic waste to light industry such as acid-sludges, manioc waste; Its microbe species is many, the effective active bacterial content high; Reach hundred million units/gram of 0.5-1, the cellulose degradation speed of becoming thoroughly decomposed is fast, materials such as the acid-sludge that is used to become thoroughly decomposed rise temperature fast, heat up high, flexibility is strong, effective.And this quick rotting agent making method is simple and easy to do, is particularly suitable for soilless culture substrate manufacturing enterprise from numerous personal.
Claims (3)
1. organic material composting microbial inoculum, it is characterized in that: it is the complex microbial community that is combined by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium, hundred million units/gram of effective active bacterial content 0.5-1; Wherein actinomycetes account for 40-60%; This microbial inoculum formulation is a pulvis, powder diameter≤0.2mm, water cut≤20%; PH5.5-7.5 has the Qu Xiangqi flavor.
2. according to claim 1 organic material composting microbial inoculum, it is characterized in that: preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the said complex microbial community.
3. the making method of the said organic material composting microbial inoculum of claim 1 is characterized in that: operation according to the following steps:
1) prepare to cultivate utensil: prepares long 30-60cm, wide 20-30cm, the some and atomizer of the plastic culture dish of 2.5-4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator deeply;
2) get the raw materials ready: prepare rice bran or wheat bran as cultivating bed material, said rice bran or wheat bran requirement are fresh, dry, nothing is gone mouldy fineness≤0.2mm; Make auxiliary material with sugar, vinegar, said sugar can be brown sugar, sucrose, glucose;
3) batching: press the proportioning of rice bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing the 2-5kg rice bran earlier, add the water stirring and be adjusted to water cut 38-42%, become bacterium material A with the bacterial classification uniform mixing again; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran and pave on concrete floor or plastic film, take by weighing needed water, stir behind the adding B by the 38-42% water cut; Evenly be sprayed on the rice bran with atomizer, stir, again A is sprinkled into while spraying; Evenly stir, adjustment pH value forms compound C to 5-7; Stacked 1-2 hour, rice bran is fully absorbed water;
4) sabot: C is packed in the vinyl disc, the thickness 3cm of C around the dish, interior thickness 2.5cm covers moist gauze, guarantees that substratum is exposed; The vinyl disc that will install C again is " groined type " and is stacked on through in the culturing room that disinfects, height 6-8 layer, and in last, insert TM respectively, indoor extension Hygrothermograph in lower floor's dish;
5) cultivation and fermentation: the cultivation and fermentation management divides three phases, and 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and the material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and the material temperature control is at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, needed this moment the upper lower burrs reversing of position is guaranteed that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and the material temperature no longer raises and when beginning to descend, in time dispel the heat, and lets the material temperature drop to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter;
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, promptly becomes the organic material composting microbial inoculum after D dries, pulverizes;
7) detect: every batch of microbial inoculum product all need detect, and the effective active bacterial content reaches hundred million units/gram of 0.5-1, fineness≤0.2mm, and water cut≤20%, pH5.5-7.5 has the Qu Xiangqi flavor;
8) packing, preservation: adopt the plastic opaque bag to pack, every bag of 0.5-2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
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