CN102397376B - Chinese medicinal composition for treating dysmenorrhea and compound supercritical fluid extract - Google Patents
Chinese medicinal composition for treating dysmenorrhea and compound supercritical fluid extract Download PDFInfo
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- CN102397376B CN102397376B CN 201110301357 CN201110301357A CN102397376B CN 102397376 B CN102397376 B CN 102397376B CN 201110301357 CN201110301357 CN 201110301357 CN 201110301357 A CN201110301357 A CN 201110301357A CN 102397376 B CN102397376 B CN 102397376B
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Abstract
The invention discloses a Chinese medicinal composition for treating dysmenorrhea. The Chinese medicinal composition comprises the following bulk pharmaceuticals in parts by weight: 1-50 parts of medicinal evodia fruit and 1-50 parts of cassia bark. The invention further discloses a compound supercritical fluid extract of the Chinese medicinal composition. The Chinese medicinal composition and the compound supercritical fluid extract have remarkable curative effects on dysmenorrhea, and are particularly suitable for treating dysmenorrhea caused by qi stagnation due to congealing cold.
Description
Technical field
The present invention relates to medical technical field, relate in particular to a kind of Chinese medicine composition and compound recipe supercritical fluid extraction thing for the treatment of dysmenorrhea.
Background technology
Dysmenorrhea is current common gynecological disease kind, and the traditional Chinese medical science thinks, the women is whenever lower abdomen pain periodically occurring before and after front or menstruation, or pain draws the waist sacrum, and very then severe pain is fainted, and nausea and vomiting is called dysmenorrhea, also claims " abdominal pain during menstruation ".Main pathogenesis can be divided into void, real two classes.Reality person is many, and qi depression to blood stasis, damp invasion of lower energizer cause QI-blood circulation not smooth because of cold and stagnate, rush to appoint and are obstructed, so-called " stagnation of QI and blood may bring about pain ", the clinical excess syndromes that show as more; Deficiency syndrome are many because of deficiency of both the liver and kidney, deficient qi and blood, and a blood deficiency is appointed in punching, and vessels of the uterus loses supports, and are to be " Rong Ze pain ", clinically show as deficiency syndrome more, but clinical the most common with cold blood stasis.Because the change of life style, the women is the young woman particularly, occupy summer in the air-conditioned room, and the winter adds body for showing the thin clothing of stature, more double normal food cold drink, or be the few food of fat-reducing, fierce because of social competition again, often cause feelings will not smooth, therefore, the pathogenesis of current clinical common dysmenorrhea is take stagnation of QI due to depression of the liver as main, women's element is reported liver-yang hyperactivity, depression is slightly arranged, then the stagnation of QI, the stagnation of QI is blood stasis then, stagnation of QI-blood, and the drink of in addition often paddling menstrual period is cold, cause caused by qi and cold stagnation, blood stasis more very, stagnation of QI and blood may bring about pain.The menorrhalgia of cold and qi stagnation type person often suffers from abdominal pain unbroken in menstrual period, some is also with sore waist and aching in the waist and the back, and the happiness temperature is afraid of cold; The severe one pale complexion, dripping cold sweat, trick is ice-cold, feels restless.In all multi-methods for the treatment of dysmenorrhea, external treatment of Chinese medicine has unique advantage, and its method that often adopts Moxibustion on Acupoints or acupoint medicine to stick is come the relieving dysmenorrhea symptom, and that acupoint medicine sticks because of it is easy to implement, and curative effect is conclusive, extensively adopts in tcm clinical practice.The acupuncture point of acupoint application treatment dysmenorrhea Chang Xuan is take SHENQUE acupoint and Guanyuan acupoint as main, and especially selecting SHENQUE acupoint the most common, the traditional Chinese medical science thinks that umbilicus (SHENQUE acupoint) is the cave of wanting of health care, the institute of gas discrepancys of the vital organs of the human body, the deposited umbilicus of the medicine uniqueness of producing effects.But, still lack at present the effectively medicine for the treatment of dysmenorrhea.
The clinical external treatment medicine of Traditional Chinese Medicine generally adopts medicinal powder, and the time spent directly spreads on corresponding site, or with vinegar furnishing pasty state, often there are the shortcomings such as drug effect that dosage form is original, can not give full play to effective ingredient in capable application again, has been difficult to adapt to the society requirement.
Supercritical fluid extraction is a kind of comparatively advanced physical extraction technology, is called for short SFE (supercritical fluid extraction).SFE utilizes supercritical carbon dioxide (CO
2) good dissolving power, with substrate and extract effective separation, extraction and purification.CO
2Safe, nontoxic, cheap liquid, supercritical CO
2Have the diffusion coefficient of similar gas, the dissolving power of liquid, under supercriticality, supercritical fluid contacts with material to be separated, its composition that successively polarity size, boiling point height and relative molecular mass is varied in size is selectively extracted, have efficient, be difficult for oxidation, pure natural, without characteristics such as chemical contaminations.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of Chinese medicine composition of effective treatment dysmenorrhea.
In addition, also provide a kind of compound recipe supercritical fluid extraction thing of Chinese medicine composition of above-mentioned treatment dysmenorrhea, this extract makes the effective ingredient of each raw material in the Chinese medicine composition bring into play better drug effect.
In order to solve the problems of the technologies described above, the present invention is achieved through the following technical solutions:
In one aspect of the invention, provide a kind of Chinese medicine composition for the treatment of dysmenorrhea, comprised the crude drug of following weight parts: Fructus Evodiae 1-50 part, Cortex Cinnamomi 1-50 part.Preferably, the consumption of described crude drug is: 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis.
The present invention makes up Fructus Evodiae and Cortex Cinnamomi, so that each efficacy of drugs produces synergism, thereby can improve the various symptoms of the dysmenorrhea that is caused by QI stagnated by cold by dispelling cold by warming the meridian, promoting the circulation of QI to relieve pain.Wherein, selecting Fructus Evodiae is because its bitter in the mouth hot in nature, and the merit of heat radiation pain relieving, stopping nausea and vomiting by lowering the adverse flow of QI is arranged, and is used for the treatment of that the liver insufficiency of stomach is cold, the diseases such as the headache due to the cloudy turbid superinverse or stomach wrist pain; Selecting Cortex Cinnamomi, is because of the Cortex Cinnamomi extremely hot in nature, acrid in the mouth, sweet, and it is fiery supporing yang to have benefit, let the fire back to its origin, dispersing cold for relieving pain, the merit of promoting blood circulation to restore menstrual flow, the clinical treatment that is usually used in sexual impotence, cold womb, trusted subordinate's cold type of pain, deficiency and coldness vomiting and diarrhoea, amenorrhea, dysmenorrhea.More than two flavor medicines complement each other, can reach the effect of good treatment dysmenorrhea, especially treat menorrhalgia of cold and qi stagnation type, effect is more remarkable.
For reaching better curative effect, Chinese medicine composition of the present invention also comprises Rhizoma Chuanxiong 1-50 part, Olibanum 1-50 part.Preferably, the consumption of each crude drug is: 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums.Selecting Rhizoma Chuanxiong, is because the hot loose temperature of Rhizoma Chuanxiong is logical, and the function blood circulation promoting and blood stasis dispelling is applicable to the disease of various stagnation of blood stasis, and particularly the Fuke Tiaojing key medicine is controlled menoxenia, amenorrhea, dysmenorrhea; Selecting Olibanum, is because the Olibanum nature and flavor are arduous, temperature, but regulating qi and activating blood, and analgesic therapy, clinical in to treat coagulation of QI-blood, trusted subordinate's pain, carbuncle pyogenic infections from tumour or sore, traumatic injury, dysmenorrhea, postnatal blood stasis twinge.Rhizoma Chuanxiong and Olibanum two flavor medicines are all the product of promoting blood flow to regulate menstruation, can jointly assist Fructus Evodiae, to strengthen the effect of blood-activating and qi-promoting pain relieving.
Fructus Evodiae, Cortex Cinnamomi dispersing cold for relieving pain in the above four Chinese medicine, the warming YANG regulating menstruation, the Rhizoma Chuanxiong promoting flow of QI and blood, the pain relieving of Olibanum promoting blood flow to regulate menstruation, all medicines match, the cold coagulation solution, stagnation of QI row, blood stasis disappears, and the dysmenorrheal caused by qi stagnation and blood stasis that causes because of sympotoms caused by cold factors is had good therapeutic effect.
Crude drug in the Chinese medicine composition of the present invention can adopt the conventional method of Chinese medicine preparation to be prepared into any conventional external preparation, for example: these crude drug pulverizes can be mixed and made into powder and transfer deposited.But in order to make in this compound recipe each raw material bring into play better drug effect, preferably adopt supercritical liquid extraction technique that the raw material the effective elements of the medicine is extracted.
In another aspect of this invention, a kind of compound recipe supercritical fluid extraction thing for the treatment of dysmenorrhea also is provided, this compound recipe supercritical fluid extraction thing can make with following method: be the Fructus Evodiae of 1-50 part and the Cortex Cinnamomi of 1-50 part with weight portion, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, be 1-100MPa at pressure, temperature was under 1-100 ℃ the condition, with co_2 supercritical fluid extraction 0.1-10 hour.
Preferably, the crude drug of described compound recipe supercritical fluid extraction thing also comprises: Rhizoma Chuanxiong 1-50 part, Olibanum 1-50 part.Preferred, the consumption of each crude drug is: 6 parts of 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs and Olibanums.
Preferably, the pressure of described extraction is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours.
Described compound recipe supercritical fluid extraction thing can add various conventional adjuvant required when preparing different dosage form, such as excipient, thickening agent, emulsifying agent, adhesive etc., be prepared into dosage form on any pharmaceutics with the method for Chinese medicinal of routine, such as cataplasma, gel, ointment, emplastrum, black plaster, rubber plaster, take back lining materials as the paster that absorbs skeleton, slow controlled release percutaneous drug administration preparation or micro/nano level percutaneous medicine-releasing system etc.Described dosage form is preferably the paster dosage form, mainly sticks in umbilical part or abdominal part.
Because the used medicine for the treatment of dysmenorrhea, its main effective ingredient mostly is volatile ingredient or fat-soluble stronger composition, therefore can adopt supercritical liquid extraction technique, the extraction active substance, and this extract generally need not further processing without organic residue, namely can be made into corresponding dosage form and uses, effectively promote preparation level, adapted to contemporary society's demand.
Chinese medicine composition of the present invention and compound recipe supercritical fluid extraction thing thereof, through zoopery and human body on probation experiment showed, have dispelling cold by warming the meridian, the function such as promoting blood circulation and stopping pain, it is evident in efficacy to be used for the treatment of dysmenorrhea, is particularly useful for treating the dysmenorrhea that causes because of QI stagnated by cold.The therapeutic effect of compound recipe supercritical fluid extraction thing of the present invention is significantly better than the medication of Chinese medicine composition powder.
The specific embodiment
The invention provides a kind of Chinese medicine composition of effective treatment dysmenorrhea, comprise the crude drug of following weight parts: Fructus Evodiae 1-50 part, Cortex Cinnamomi 1-50 part.Preferably, the consumption of each crude drug is: 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis.
For reaching better curative effect, Chinese medicine composition of the present invention also comprises Rhizoma Chuanxiong 1-50 part, Olibanum 1-50 part.Most preferred, the consumption of each crude drug is: 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums.
For the effective ingredient that makes each raw material in the Chinese medicine composition is brought into play drug effect more fully, and remove invalid or even the composition of side effect is arranged, the present invention adopts the supercritical fluid extraction method that the effective ingredient in the crude drug is extracted.The present invention treats the compound recipe supercritical fluid extraction thing of dysmenorrhea, available following method makes: be the Fructus Evodiae of 1-50 part and the Cortex Cinnamomi of 1-50 part with weight portion, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, be 1-100MPa at pressure, temperature was under 1-100 ℃ the condition, with co_2 supercritical fluid extraction 0.1-10 hour.
Preferably, the crude drug of compound recipe supercritical fluid extraction thing also comprises: Rhizoma Chuanxiong 1-50 part, Olibanum 1-50 part.Preferred, the consumption of each crude drug is: 6 parts of 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs and Olibanums.
Preferably, the extracting pressure for preparing this compound recipe supercritical fluid extraction thing is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours.
The present invention is further detailed explanation below by embodiment.
The preparation of embodiment 1 compound recipe supercritical fluid extraction thing
With 10 parts of Fructus Evodiaes, 10 portions of Cortex Cinnamomis, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, uses co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours, collect extract, it is prepared into the dosage form of stipulating on any pharmaceutics.
The preparation of embodiment 2 compound recipe supercritical fluid extraction things
With 1 part of Fructus Evodiae, 1 portion of Cortex Cinnamomi, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, uses co_2 supercritical fluid extraction, extracting pressure is 20MPa, and temperature is 35 ℃, and extraction time is 0.1 hour, collect extract, it is prepared into the dosage form of stipulating on any pharmaceutics.
The preparation of embodiment 3 compound recipe supercritical fluid extraction things
With 50 parts of Fructus Evodiaes, 50 portions of Cortex Cinnamomis, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, uses co_2 supercritical fluid extraction, extracting pressure is 10MPa, and temperature is 100 ℃, and extraction time is 8 hours, collect extract, it is prepared into the dosage form of stipulating on any pharmaceutics.
The preparation of paster dosage form of embodiment 4 compound recipe supercritical fluid extraction beyond the region of objective existences
With 10 parts of Fructus Evodiaes, 10 portions of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours, collect extract, it is coated on the good textile backing pad of absorbability, adhere on the Breathable adhesive tape again, the time spent directly sticks in umbilical part or abdominal part and gets final product.
The preparation of the plaster dosage form of embodiment 5 compound recipe supercritical fluid extraction things
With 5 parts of Fructus Evodiaes, 5 portions of Cortex Cinnamomis, 1 part of Rhizoma Chuanxiong, 1 part of Olibanum, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 1MPa, and temperature is 90 ℃, extraction time is 10 hours, collect extract, add the used pharmaceutical necessities of preparation plaster, coat on the Breathable adhesive tape, be prepared into the plaster dosage form, the time spent directly sticks in umbilical part or abdominal part and gets final product.
The preparation of the acupoint plaster dosage form of embodiment 6 compound recipe supercritical fluid extraction things
With 30 parts of Fructus Evodiaes, 30 portions of Cortex Cinnamomis, 10 parts of Rhizoma Chuanxiongs, 10 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 35MPa, and temperature is 60 ℃, extraction time is 8 hours, collect extract, add the used adjuvant of preparation, make the moderate mastic of stickiness, be filled in the circle storage storehouse of acupoint plaster, the time spent directly aims at drug depot the positions such as SHENQUE acupoint or Guanyuan acupoint and sticks.
The preparation of the cataplasma type of embodiment 7 compound recipe supercritical fluid extraction things
With 20 parts of Fructus Evodiaes, 20 portions of Cortex Cinnamomis, 8 parts of Rhizoma Chuanxiongs, 8 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, extraction time is 5 hours, collect extract, add filler and cross-linking agent etc., make the moderate mastic of stickiness, coat on the back lining materials, the time spent directly sticks in umbilical part or abdominal part.
The preparation of the ointment dosage form of embodiment 8 compound recipe supercritical fluid extraction things
With 50 parts of Fructus Evodiaes, 50 portions of Cortex Cinnamomis, 50 parts of Rhizoma Chuanxiongs, 50 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 100MPa, and temperature is 80 ℃, and extraction time is 9 hours, collect extract, add the required adjuvant of ointment in the preparation medicament, make the moderate mastic of hardness, the time spent can be coated on umbilical part or abdominal part.
The preparation of the gel dosage form of embodiment 9 compound recipe supercritical fluid extraction things
With 10 parts of Fructus Evodiaes, 10 portions of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours, collect extract, add the required adjuvant of gel in the preparation medicament, make the gel dosage form, the time spent can be coated on umbilical part or abdominal part.
The preparation of the slow controlled release percutaneous dosing dosage form of embodiment 10 compound recipe supercritical fluid extraction things
With 10 parts of Fructus Evodiaes, 10 portions of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours, collect extract, add the required adjuvant of preparation slow releasing preparation, be prepared into sustained-release preparation, to be for external application.
The preparation of the novel micrometer/nanometer percutaneous dosing dosage form of embodiment 11 compound recipe supercritical fluid extraction things
With 10 parts of Fructus Evodiaes, 10 portions of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs, 6 parts of Olibanums, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, use co_2 supercritical fluid extraction, extracting pressure is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours, collect extract, add the required adjuvants of preparation such as preparation micron, nanoscale, be prepared into pastille micro-/ nano granule, to be for external application.
The zoopery of embodiment 12 compound recipe supercritical fluid extraction things
1. dysmenorrhea model
Animal more options mice or the rat of preparation dysmenorrhea model are generally adopted the whole animal model.Sensitization medicine more options diethylstilbestrol also has the selection estradiol benzoate, and the dysmenorrhea model utilization rate of preparation take 4d as one-period is higher.Diethylstilbestrol is the non steroidal estrogen material of synthetic, can produce all pharmacology and the therapeutical effect identical with natural estradiol, can strengthen uterine contraction, improve the uterus to the sensitivity of oxytocin, estradiol benzoate is the derivant of estradiol, the uterus is increased the sensitivity of oxytocin, strengthen the contraction of uterine smooth muscle.The oxytocin of induced pain medicine more options, it can directly act on myometril cell and cause uterine contraction, and simultaneously stimulon endometrial cells release PGF-α causes dysmenorrhea.Whole dysmenorrhea model evaluation index adopts writhing response (mouse web portion shrinks indent, and trunk and hind leg stretch, buttocks and a side limbs inward turning) number of times and writhing Mus occurrence rate more.
2. laboratory animal
75 of Kunming mouses, female; 130 of Kunming mouses, male and female half and half, Mus 4～5wk in age, body weight 18～22g is provided by the Shanghai medicine.Experiment is carried out 20～23 ℃ of room temperatures, relative humidity 60%～70% at Shanghai Univ. of Traditional Chinese Medicine's cleaning level Animal Lab..
3. experimental technique
3.1 the grouping of dysmenorrhea model Mus
Get 45 of the female Kunming mouses of body weight 18～22g, be divided at random 3 groups by body weight, 15 every group.That is: model control group A; Commercially available TONGJING TIE group B; Compound recipe supercritical fluid extraction thing group C of the present invention.
Get 45 of the female Kunming mouses of body weight 18～22g, be divided at random 3 groups by body weight, 15 every group.That is: model control group A; The thick medicated powder administration of Chinese medicine composition of the present invention group B; Compound recipe supercritical fluid extraction thing administration group C of the present invention.
3.2 the preparation of depilatory cream
Get liquid detergent, sodium sulfide, starch (1: 3: 7) to grinding in the alms bowl, pour a small amount of hot water into and grind even, to being thick and get final product.
3.3 the model manufacturing of dysmenorrhea mice and evaluation methodology
Each organizes equal subcutaneous injection estradiol benzoate, continuous 4d, and 0.2mg (0.1ml)/only, to improve the uterus sensitivity.B organized the depilation of mice and C group mouse web portion in the 4th day, each organizes the mice fasting, can't help water.In the 5th day with corresponding medicine paste spread on B group mice, C organizes mouse web portion; Behind the 2h, each is organized, and the equal lumbar injection oxytocin of mice 2U (0.2ml)/only, the observation writhing reflects number of animals and the interior writhing number of times of 30min that (abdominal part shrinks indent, and trunk and hind leg stretch, buttocks and a side limbs inward turning) occurs.
3.4 statistical method
All data means
± standard deviation (s) expression is carried out statistical procedures with q check between group, and there was no significant difference is relatively arranged.
4. experimental result
4.1 compound recipe supercritical fluid extraction thing experimental result of the present invention sees the following form shown in 1:
The impact that table 1 compound recipe supercritical fluid extraction thing is suffered from abdominal pain on the dysmenorrhea model Mus (n=15,
)
Annotate: compare * P＜0.05, * * P＜0.01 with model control group.
Experimental result shows that commercially available TONGJING TIE administration group and compound recipe supercritical fluid extraction thing administration group be the violent contraction in energy antagonism oxytocin induced mice uterus all.Two groups and model control group comparison all have analgesic activity to the rat primary dysmenorrhea, and compound recipe supercritical fluid extraction thing group better effects if.Behind the compound recipe supercritical fluid extraction thing transdermal administration mouse writhing number of times obviously reduce faster, and observe in the experiment visible its incubation period long, recover very fast.
4.2 compound recipe supercritical fluid extraction thing experimental result of the present invention sees the following form shown in 2:
The impact that table 2 compound recipe supercritical fluid extraction thing is suffered from abdominal pain on the dysmenorrhea model Mus (n=15,
)
Annotate: compare * P＜0.05, * * P＜0.01 with model control group.
Experimental result shows that Chinese medicine composition thick medicated powder administration group and compound recipe supercritical fluid extraction thing administration group be the violent contraction in energy antagonism oxytocin induced mice uterus all.And two groups and model control group comparison all have preferably analgesic activity to the rat primary dysmenorrhea.The mouse writhing number of times obviously reduces sooner behind the compound recipe supercritical fluid extraction thing transdermal administration, and observes in the experiment that visible its recovers very fast, incubation period is longer.Compound recipe supercritical fluid extraction thing is easier to Transdermal absorption than thick medicated powder, improves drug effect.
The pre-clinical trial of embodiment 13 compound recipe supercritical fluid extraction things
For investigating the drug action of compound recipe supercritical fluid extraction thing treatment dysmenorrhea of the present invention, totally 78 patients have carried out clinical practice to compound recipe supercritical fluid extraction thing preparation.
1. experimental technique
When menstruation will be gone or pain is shown effect, put on the back lining materials with compound recipe supercritical fluid extraction thing, spread on the SHENQUE acupoint position, the next day, change dressings 1 time, pasted per month 6 days, and continuous 3 months is a course for the treatment of.Note simultaneously menstrual period health, regulating spirit and emotion by oneself, nourishing the heart to keep a sound mind.
2. efficacy assessment standard and result
According to the efficacy assessment standard in " traditional Chinese medical science disease Standardization of diagnosis and curative effect " of State Administration of Traditional Chinese Medicine's nineteen ninety-five promulgation.Cure: pain disappears, and continuous 3 menstrual cycle have no recurrence; Take a turn for the better: pain relief or pain disappear, but can not keep more than 3 months.Do not heal: pain has no improvement.
3. result
Add up altogether satisfactory patient's 78 examples, cure 59 examples, 13 examples that take a turn for the better, invalid 6 examples, cure rate is 75.6%, total effective rate 92.3%.
The above embodiment has only expressed embodiments of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (4)
1. Chinese medicine compound supercritical fluid extraction thing for the treatment of menorrhalgia of cold and qi stagnation type, it is characterized in that, described compound recipe supercritical fluid extraction thing is made by following methods: with crude drug Fructus Evodiae 1-50 part, Cortex Cinnamomi 1-50 part, Rhizoma Chuanxiong 1-50 part and Olibanum 1-50 part of following weight parts, pulverizing is placed in the extraction kettle of supercritical fluid extraction instrument, be 1-100MPa at pressure, temperature was under 1-100 ℃ the condition, with co_2 supercritical fluid extraction 0.1-10 hour.
2. Chinese medicine compound supercritical fluid extraction thing according to claim 1 is characterized in that the consumption of described crude drug is: 6 parts of 10 parts of Fructus Evodiaes, 10 parts of Cortex Cinnamomis, 6 parts of Rhizoma Chuanxiongs and Olibanums.
3. Chinese medicine compound supercritical fluid extraction thing according to claim 1 is characterized in that, the pressure of described extraction is 25MPa, and temperature is 50 ℃, and extraction time is 4 hours.
4. Chinese medicine compound supercritical fluid extraction thing according to claim 1 is characterized in that described extract can be made into the dosage form on any pharmaceutics.
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| CN106212820A (en) * | 2016-08-11 | 2016-12-14 | 亳州市益众堂保健食品有限公司 | A kind of dysmenorrhea women tea and preparation method thereof |
| CN107596137A (en) * | 2017-08-25 | 2018-01-19 | 李占 | A kind of women preserves youthful looks pellet and preparation method thereof |
| CN107596116A (en) * | 2017-08-25 | 2018-01-19 | 李占 | A kind of male health preserving pellet and preparation method thereof |
| CN108653674A (en) * | 2018-07-10 | 2018-10-16 | 贵州东仪医疗器械有限公司 | It is a kind of to be used to treat by the patch and its preparation method and application of the caused abdominal pain of gynecological disease |
| CN109464644A (en) * | 2018-12-29 | 2019-03-15 | 深圳市淳睿贸易有限公司 | Radix Angelicae Sinensis motherwort massage oil, preparation method and its application |
| CN110732016A (en) * | 2019-12-12 | 2020-01-31 | 河南中医药大学 | A topical essential oil of fructus Cinnamomi Immaturus for treating dysmenorrhea |
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