CN102388145A - 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 - Google Patents

用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Download PDF

Info

Publication number
CN102388145A
CN102388145A CN2009801551526A CN200980155152A CN102388145A CN 102388145 A CN102388145 A CN 102388145A CN 2009801551526 A CN2009801551526 A CN 2009801551526A CN 200980155152 A CN200980155152 A CN 200980155152A CN 102388145 A CN102388145 A CN 102388145A
Authority
CN
China
Prior art keywords
cell
cells
antibody
method described
natural killer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801551526A
Other languages
English (en)
Chinese (zh)
Inventor
J·C·洛夫
N·范阮达若詹
B·居尔格
B·沃克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Massachusetts Institute of Technology
Original Assignee
General Hospital Corp
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp, Massachusetts Institute of Technology filed Critical General Hospital Corp
Priority to CN201510140107.0A priority Critical patent/CN104745669A/zh
Publication of CN102388145A publication Critical patent/CN102388145A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN2009801551526A 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Pending CN102388145A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510140107.0A CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14610609P 2009-01-21 2009-01-21
US61/146,106 2009-01-21
PCT/US2009/050411 WO2010085275A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201510140107.0A Division CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Publications (1)

Publication Number Publication Date
CN102388145A true CN102388145A (zh) 2012-03-21

Family

ID=42356144

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2009801551526A Pending CN102388145A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法
CN201510140107.0A Pending CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201510140107.0A Pending CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Country Status (11)

Country Link
US (1) US9244071B2 (https=)
EP (1) EP2389446B1 (https=)
JP (2) JP2012515548A (https=)
KR (1) KR20110134386A (https=)
CN (2) CN102388145A (https=)
AU (1) AU2009338127B2 (https=)
CA (1) CA2689681C (https=)
IL (1) IL213938A0 (https=)
RU (1) RU2532228C2 (https=)
SG (2) SG10201401643PA (https=)
WO (1) WO2010085275A1 (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105593376A (zh) * 2013-08-26 2016-05-18 哈佛学院院长等 免疫细胞及其它细胞的测定
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
CN110998330A (zh) * 2017-06-29 2020-04-10 迈博太科产品公司 用于分析荧光免疫斑点测定的方法和系统
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US12391914B2 (en) 2022-11-07 2025-08-19 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5675638B2 (ja) 2008-12-04 2015-02-25 マサチューセッツ インスティテュート オブ テクノロジー アレルギー反応を診断するための方法
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
SG10201401643PA (en) 2009-01-21 2014-08-28 Massachusetts Inst Technology Methods for assessing cytotoxicity of single cells
EP2646798B1 (en) 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
US10571475B2 (en) * 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
WO2013090404A2 (en) * 2011-12-13 2013-06-20 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
EP4148118A1 (en) * 2012-08-24 2023-03-15 Yale University System, device and method for high-throughput multi-plexed detection
RU2019136370A (ru) 2014-01-31 2019-11-25 Агбайоми, Инк. Модифицированные средства биоконтроля и их применение
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US10488321B2 (en) 2015-03-19 2019-11-26 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
WO2016154620A1 (en) 2015-03-26 2016-09-29 University Of Houston System Integrated functional and molecular profiling of cells
US12071663B2 (en) 2016-01-15 2024-08-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
US20190106666A1 (en) * 2016-03-30 2019-04-11 Sony Corporation Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells
CA3027317A1 (en) 2016-06-14 2017-12-21 Cellply S.R.L. Screening kit and method
CN109563539A (zh) 2016-06-15 2019-04-02 慕尼黑路德维希马克西米利安斯大学 使用dna纳米技术的单分子检测或定量
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP3532600A4 (en) * 2016-10-19 2020-09-16 General Automation LAB Technologies Inc. HIGH RESOLUTION SYSTEMS, KITS, DEVICES AND METHODS FOR SCREENING MICRO-ORGANISMS AND OTHER HIGH-PERFORMANCE MICROBIOLOGICAL APPLICATIONS
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
JP2021505157A (ja) 2017-12-07 2021-02-18 マサチューセッツ インスティテュート オブ テクノロジー 単一細胞分析
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
EP3911953A4 (en) * 2019-01-16 2022-08-31 Yantai AusBio Laboratories Co., Ltd. AUTOMATED LIQUID HANDLING SYSTEM AND PROCESS FOR SEPARATION OF BIOLOGICAL SAMPLES FOR MICROSCOPIC ANALYZES
US11357787B2 (en) 2019-02-18 2022-06-14 Nb Health Laboratory Co., Ltd. Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007035633A2 (en) * 2005-09-16 2007-03-29 President & Fellows Of Harvard College Screening assays and methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68507A (en) 1982-05-10 1986-01-31 Univ Bar Ilan System and methods for cell selection
SU1650094A1 (ru) * 1987-12-29 1991-05-23 Научно-исследовательский институт проктологии Способ диагностики прот женности активного воспалени в толстой кишке при неспецифическом звенном колите
US6410252B1 (en) 1995-12-22 2002-06-25 Case Western Reserve University Methods for measuring T cell cytokines
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
AU4200900A (en) * 1999-04-09 2000-11-14 Human Genome Sciences, Inc. 49 human secreted proteins
EP1192176A4 (en) * 1999-06-11 2003-03-05 Human Genome Sciences Inc 49 HUMAN SECRETED PROTEINS
US7332286B2 (en) * 2001-02-02 2008-02-19 University Of Pennsylvania Peptide or protein microassay method and apparatus
US20020141906A1 (en) 2001-03-30 2002-10-03 American Type Culture Collection Microtiter plate sealing cover with well identifiers
US20110124520A1 (en) 2008-05-30 2011-05-26 Massachuesetts Institute Of Technology Compositions and Methods for Spatial Separation and Screening of Cells
JP5675638B2 (ja) 2008-12-04 2015-02-25 マサチューセッツ インスティテュート オブ テクノロジー アレルギー反応を診断するための方法
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
SG10201401643PA (en) 2009-01-21 2014-08-28 Massachusetts Inst Technology Methods for assessing cytotoxicity of single cells
US8569046B2 (en) 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007035633A2 (en) * 2005-09-16 2007-03-29 President & Fellows Of Harvard College Screening assays and methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ASIER SÁEZ-CIRIÓN ET AL.: "HIV controllers exhibit potent CD8 T cell capacity to suppress HIV infection ex vivo and peculiar CTL activation phenotype", 《"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105593376A (zh) * 2013-08-26 2016-05-18 哈佛学院院长等 免疫细胞及其它细胞的测定
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
CN110998330A (zh) * 2017-06-29 2020-04-10 迈博太科产品公司 用于分析荧光免疫斑点测定的方法和系统
CN110998330B (zh) * 2017-06-29 2024-05-10 迈博太科股份公司 用于分析荧光免疫斑点测定的方法和系统
US12092577B2 (en) 2017-06-29 2024-09-17 Mabtech Ab Method and system for analysing FluoroSpot assays
US12391914B2 (en) 2022-11-07 2025-08-19 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions

Also Published As

Publication number Publication date
AU2009338127B2 (en) 2015-11-26
JP2012515548A (ja) 2012-07-12
EP2389446B1 (en) 2017-07-05
SG172889A1 (en) 2011-08-29
CA2689681A1 (en) 2010-07-21
EP2389446A4 (en) 2012-10-03
AU2009338127A1 (en) 2011-07-28
US20120149592A1 (en) 2012-06-14
WO2010085275A1 (en) 2010-07-29
CN104745669A (zh) 2015-07-01
EP2389446A1 (en) 2011-11-30
JP6017620B2 (ja) 2016-11-02
US9244071B2 (en) 2016-01-26
RU2011134898A (ru) 2013-02-27
IL213938A0 (en) 2011-07-31
KR20110134386A (ko) 2011-12-14
SG10201401643PA (en) 2014-08-28
CA2689681C (en) 2017-08-29
JP2015144617A (ja) 2015-08-13
RU2532228C2 (ru) 2014-10-27

Similar Documents

Publication Publication Date Title
JP6017620B2 (ja) 単一細胞の細胞傷害性を査定するための組成物および方法
Clute et al. Cross-reactive influenza virus–specific CD8+ T cells contribute to lymphoproliferation in Epstein-Barr virus–associated infectious mononucleosis
Sun et al. Immune memory redefined: characterizing the longevity of natural killer cells
CN114616467B (zh) 筛选原代人类细胞中的同源t细胞和表位反应性的高通量方法
US8975069B2 (en) Method for identifying antigen-specific regulatory T cells
Palendira et al. Primary immunodeficiencies and the control of Epstein–Barr virus infection
Heutinck et al. Virus-specific CD8+ T cells cross-reactive to donor-alloantigen are transiently present in the circulation of kidney transplant recipients infected with CMV and/or EBV
Sharma et al. Mucosal-associated invariant T cells develop an innate-like transcriptomic program in anti-mycobacterial responses
Billingsley et al. Characterization of CD8+ T cell differentiation following SIVΔnef vaccination by transcription factor expression profiling
Nyanhete et al. HLA class II-restricted CD8+ T cells in HIV-1 virus controllers
Kocher et al. Vaccination-induced T cell responses maintain polyclonality with high antigen receptor avidity
Kocher et al. Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity
Kocher et al. Antigen-reactive CD4+ T cells after SARS-CoV-2 vaccination show divergent phenotypic states with or without restimulation bias
Meckiff Evolution of the human CD4+ T cell response to Epstein-Barr virus infection-analysis of systemic and local immune responses
Richard Toll-like receptor (TLR) 7 and TLR8 stimulation of mucosal-associated invariant T cells and gamma delta T cells: a role in HIV susceptibility
Iancu et al. Profile of a serial killer: cellular and molecular approaches to study individual cytotoxic T‐cells following therapeutic vaccination
Kahia Defining early CD8+ T cell responses during acute and chronic viral infection using single-cell RNA-seq analysis
Sherman Autoimmunity-associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity
James Molecular and Cellular Mechanisms of Mycobacterial Glycolipid Recognition by Human T Cells
Phetsouphanh Novel approaches to the characterization of antigen-specific CD4+ T cells in HIV-1 infection
Preece Harnessing CD4+ T cell effectors for lymphoma therapy
Catry Specific T-and B-cell responses against Human cytomegalovirus after Hematopoietic stem cell transplantation
Schwele Human cytomegalovirus-specific regulatory and effctor T cells are clonally identical
Nguyen Characterization of naïve immune cell subsets important in HIV/SIV pathogenesis as a baseline for RNAseq deconvolution
Clutton HIV-specific interleukin-10 responses and immune modulation

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20120321