CN102274265B - Compound propolis composition for treating livestock mastitis and preparation method thereof - Google Patents
Compound propolis composition for treating livestock mastitis and preparation method thereof Download PDFInfo
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- CN102274265B CN102274265B CN2011101982511A CN201110198251A CN102274265B CN 102274265 B CN102274265 B CN 102274265B CN 2011101982511 A CN2011101982511 A CN 2011101982511A CN 201110198251 A CN201110198251 A CN 201110198251A CN 102274265 B CN102274265 B CN 102274265B
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Abstract
The invention relates to a compound propolis composition for treating livestock mastitis and a preparation method thereof. The compound propolis composition for treating livestock mastitis is prepared by evenly mixing the following components in parts by weight: 5-20 parts of propolis extract (alcohol extract), 5-10 parts of taraxacum extract (alcohol extract), 5-10 parts of honeysuckle flower extract (alcohol extract), 2 parts of menthol, 40 parts of 75% alcohol and 18-43 parts of distilled water. The preparation formulation of the compound propolis composition for treating livestock mastitis is a spraying agent. The preparation method comprises the following steps: putting the propolis extract (alcohol extract) into 20 parts of 75% alcohol, and uniformly stirring for 10-25 minutes; taking 20 parts of 75% alcohol, sequentially adding 5-10 parts of taraxacum extract (alcohol extract) and 5-10 parts of honeysuckle flower extract (alcohol extract), and dissolving by stirring; and merging the solutions prepared in the steps above, uniformly stirring, standing for 15-20 minutes, adding 2 parts of menthol and 18-43 parts of distilled water after the medicinal liquor becomes clear, uniformly stirring, and filtering. The invention has the advantages of reasonable formula, simple preparation process and obvious curative effect.
Description
Technical field
The present invention relates to a kind of compound bee-glue composition spray for the treatment of garget and preparation method thereof, particularly a kind of compound bee-glue composition spray for the treatment of garget and preparation method thereof.
Background technology
Garget by many reasons cause, female animal before just before giving birth or the common a kind of multiple diseases of pregnancy duration, multiplely be born in the lactation period of breast with domestic animal.Primary disease is the commonly encountered diseases frequently-occurring disease in the livestock culturing process for breast, and the life security that sometimes even jeopardizes female animal and newborn animal thereof is to cause one of breast disease the most serious by the domestic animal economic loss.
This sick paathogenic factor is a lot, the cause of disease mostly is mechanical irritation, the damage of chemicals rationality and cause pathogeny imcrobe infection etc., wherein cause pathogeny imcrobe infection is this sick main cause, its pathogen is mainly escherichia coli, streptococcus, staphylococcus aureus etc., and this disease spreads all over five continents more or less a hundred country.In decades, comprise all over the world China successively the medicines such as penicillins, chloromycetin, aminoglycosides, quinolones list in the treatment this sick choice drug in, due to a kind of (class) antibiotic of prolonged and repeated use or antimicrobial drug, cause this pathogenic microorganism to produce drug resistance to medicine, reduced curative effect, also made drug residue strengthen.
Current medicine effect is outstanding not, with the existing drug residue of western medicine, can produce drug resistance again, and environment and human health are constituted a serious threat.Present application method also mostly is injection, to domestic animal stress be very large, therefore research and develop a kind of specially good effect for the treatment of garget and conveniently use, medicine that irritability is less is one of this field scientific research technical staff new problem anxious to be resolved.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point, provide that a kind of reasonable recipe, preparation are simple, successful, effectively treat compound bee-glue composition spray of garget and preparation method thereof.
Implementation of the present invention is as follows for achieving the above object: a kind of compound bee-glue composition spray for the treatment of garget is characterized in that by following components in weight portion number than evenly being mixed: 5～20 parts of propolis extracts (alcohol extraction), 5～10 parts of Herba Taraxaci extracts (alcohol extraction), 5～10 parts of Flos Lonicerae extracts (alcohol extraction), 2 parts of menthols, 40 parts of 75% ethanol, 18～43 parts of distilled water.
Described various component optimum ratio amount is: 10 parts of propolis extracts (alcohol extraction), 7 parts of Herba Taraxaci extracts (alcohol extraction), 8 parts of Flos Lonicerae extracts (alcohol extraction), 2 parts of menthols, 40 parts of 75% ethanol, 33 parts of distilled water.
A kind of preparation method for the treatment of the compound bee-glue composition spray of garget is characterized in that carrying out as follows:
(1) take by weight 5～20 parts of propolis extracts (alcohol extraction), 5～10 parts of Herba Taraxaci extracts (alcohol extraction), 5～10 parts of Flos Lonicerae extracts (alcohol extraction), 2 parts of menthols, 40 parts of 75% ethanol;
(2) described propolis extract (alcohol extraction) is put into to 75% ethanol of 20 parts, stirred 10～25 minutes to dissolving;
(3) get 20 parts of 75% ethanol, add successively Herba Taraxaci extract (alcohol extraction), the Flos Lonicerae extract (alcohol extraction) of described umber, stirring and dissolving;
(4) merge the solution that above-mentioned (2) and (3) step make, stir, keep 15～20 minutes, add 2 parts of described menthols, 18～43 parts of distilled water after medicinal liquid is clear and bright, the filtration that stirs.
The said composition dosage form is spray.
The medicinal property of each component is as follows:
The main component of propolis extract (alcohol extraction) has flavonoid, the rare class of terpene, organic acid, armaticity aldehydes and several amino acids, enzyme, vitamin, mineral etc.Its main component can not only enhancing body non-specific and specific immune function, and various bacteria, fungus, virus, protozoon are had and suppress or killing action.Propolis also contains 34 kinds of trace element, but the regeneration capacity of zinc acceleration of wound tissue wherein, and the skin ulcer face does not stay any cicatrix after curing.In addition, after the propolis alcoholic solution is smeared local mucous membrane, can form layer protecting film, ulcer surface is isolated with pathogenic microorganism under the protection of film, prevents secondary infection, and accelerates the healing of skin ulcer face under the effect of zinc.
The main component of Herba Taraxaci extract (alcohol extraction) has taraxasterol, choline, inulin, vitamin, taraxacin etc.Its main component has stronger killing action to staphylococcus aureus, Hemolytic streptococcus, escherichia coli etc.; Fungus, bacillus pyocyaneus, Bacillus proteus, dysentery bacterium etc. are had to certain inhibitory action.Be improved in addition the effect of domestic animal immunity, hepatic cholagogic.
The main component of Flos Lonicerae extract (alcohol extraction) has chlorogenic acid, glycoside, flavonoid, volatile oil composition.Glycosides composition comprises Saponin, iridoid glycoside etc., and flavone compound has 5-hydroxyl-3 ', 4, the 7-trimethoxy flavone, glycoside has Quercetin-3-O-b-D-glycoside, hyperin etc., and in volatile oil, containing composition more than 30 kinds, main component is Flos Lonicerae alcohol and linalool.The Flos Lonicerae ethanol extract all has certain inhibitory action to the multiple Grain-positives such as staphylococcus aureus, Staphylococcus albus, Hemolytic streptococcus, Bacillus typhi, Salmonella paratyphi, escherichia coli, dysentery bacterium, Bacillus proteus, bacillus pyocyaneus, tubercule bacillus, vibrio cholera and negative bacterium.Also have in addition certain antiinflammatory, refrigeration function.
Menthol and ethanol (proportioning 1: 15) are the best of breed of transdermal enhancer, three's extract are had to the effect of good promotion Drug Percutaneous Absorption.
The extract that above-mentioned three belongs to a different category, the action principle difference, but the effects of the pathogen such as staphylococcus, streptococcus, escherichia coli is arranged, and have other antibacterial actions concurrently, limiting proportioning by the present invention, to prepare effect better, can prevent and treat the infected animal secondary infection.
The invention has the beneficial effects as follows: reasonable recipe, preparation is simple, and substep mixes more even, the curative effect highly significant.The present invention is for adopting compound preparation, adopt alcohol extracting method and molecular pharmacology method, optimize these three kinds of medicine-propolis extracts (alcohol extraction), Flos Lonicerae extract (alcohol extraction) and Herba Taraxaci extract (alcohol extraction), and make them according to best proportioning, form compound preparation, clinical practice proves that the treatment garget is had to specially good effect.
The specific embodiment
Below in conjunction with preferred embodiment, to according to the specific embodiment provided by the invention, details are as follows:
Embodiment 1
A kind of compound bee-glue composition spray for the treatment of garget and preparation method thereof, is characterized in that carrying out as follows: (every part by 10g)
(1) take by weight propolis extract (alcohol extraction) 50g, Herba Taraxaci extract (alcohol extraction) 50g, Flos Lonicerae extract (alcohol extraction) 50g;
(2) described propolis extract (alcohol extraction) is put into to 200g 75% ethanol, stir 10～25 minutes to dissolving;
(3) get 75% ethanol 200g, add successively Herba Taraxaci extract (alcohol extraction), the Flos Lonicerae extract (alcohol extraction) of described umber weight, stirring and dissolving;
(4) merge the solution that above-mentioned (2) and (3) step make, stir, keep 15～20 minutes, add 20 parts of described menthols, 430 parts of distilled water after medicinal liquid is clear and bright, the filtration that stirs.
Embodiment 2
A kind of compound bee-glue composition spray for the treatment of garget and preparation method thereof, is characterized in that carrying out as follows: (every part by 10g)
(1) take by weight propolis extract (alcohol extraction) 150g, Herba Taraxaci extract (alcohol extraction) 70g, Flos Lonicerae extract (alcohol extraction) 70g;
(2) described propolis extract (alcohol extraction) is put into to 200g 75% ethanol, stir 10～25 minutes to dissolving;
(3) get 75% ethanol 200g, add successively Herba Taraxaci extract (alcohol extraction), the Flos Lonicerae extract (alcohol extraction) of described umber weight, stirring and dissolving;
(4) merge the solution that above-mentioned (2) and (3) step make, stir, keep 15～20 minutes, add 20 parts of described menthols, 290 parts of distilled water after medicinal liquid is clear and bright, the filtration that stirs.
Embodiment 3
A kind of compound bee-glue composition spray for the treatment of garget and preparation method thereof, is characterized in that carrying out as follows: (every part by 10g)
(1) take by weight propolis extract (alcohol extraction) 200g, Herba Taraxaci extract (alcohol extraction) 100g, Flos Lonicerae extract (alcohol extraction) 100g;
(2) described propolis extract (alcohol extraction) is put into to 200g 75% ethanol, stir 10～25 minutes to dissolving;
(3) get 75% ethanol 200g, add successively Herba Taraxaci extract (alcohol extraction), the Flos Lonicerae extract (alcohol extraction) of described umber weight, stirring and dissolving;
(4) merge the solution that above-mentioned (2) and (3) step make, stir, keep 15～20 minutes, add 20 parts of described menthols, 180 parts of distilled water after medicinal liquid is clear and bright, the filtration that stirs.
Clinical trial:
Product of the present invention is sprayed directly on to and suffers from the poultry breast surface, 2～3 times/time, 2 times on the one, be used in conjunction 3～5.
Table 1 is the field applicable cases contrast of the present invention and other medicines treatment swine udder:
| Numbering | Group | Mortality rate % | Cure rate % | Symptom rate % is arranged during end |
| Ⅰ | The blank group | 5.3 | - | 20.2 |
| Ⅱ | Injection penicillin group | 3.5 | 81.9 | 14.6 |
| Ⅲ | Enrofloxacin spray group | 3.2 | 84.2 | 12.6 |
| Ⅳ | Of the present invention group | 0 | 93.7 | 6.3 |
Table 2 is field applicable cases contrasts of the present invention and other medicines treatment mammitis of cow:
| Numbering | Group | Mortality rate % | Cure rate % | Symptom rate % is arranged during end |
| Ⅰ | The blank group | 2.0 | - | 18.6 |
| Ⅱ | Injection penicillin group | 1.5 | 85.0 | 13.5 |
| Ⅲ | Enrofloxacin spray group | 1.2 | 87.6 | 11.2 |
| Ⅳ | Of the present invention group | 0 | 95.8 | 4.2 |
This result of the test statistical analysis, the present invention treats the compound bee-glue composition spray treatment group of garget and compares difference extremely significantly (P<0.01) with penicillin injection liquid treatment group, enrofloxacin spray treatment group.
Above-mentioned detailed description of compound bee-glue composition spray of this treatment garget and preparation method thereof being carried out with reference to embodiment; illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under general plotting of the present invention, within should belonging to protection scope of the present invention.
Claims (3)
1. a compound bee-glue composition for the treatment of garget, it is characterized in that by following components in weight portion number than evenly being mixed: 5～20 parts of extractive of propolis alcohol, 5～10 parts of taraxol extracts, 5～10 parts of Flos Lonicerae alcohol extracts, 2 parts of menthols, 40 parts of 75% ethanol, 18～43 parts of distilled water, the said composition dosage form is spray.
2. a kind of compound bee-glue composition for the treatment of garget according to claim 1, it is characterized in that described various component optimum ratio amount is: 10 parts of extractive of propolis alcohol, 7 parts of taraxol extracts, 8 parts of Flos Lonicerae alcohol extracts, 2 parts of menthols, 40 parts of 75% ethanol, 33 parts of distilled water.
3. the preparation method of the compound bee-glue composition of a treatment garget according to claim 1 is characterized in that carrying out as follows:
(1) take by weight 5～20 parts of extractive of propolis alcohol, 5～10 parts of taraxol extracts, 5～10 parts of Flos Lonicerae alcohol extracts, 2 parts of menthols, 40 parts of 75% ethanol;
(2) described extractive of propolis alcohol is put into to 75% ethanol of 20 parts, stirred 10～25 minutes to uniform dissolution;
(3) get 20 parts of 75% ethanol, add successively 5～10 parts of described taraxol extracts, 5～10 parts of Flos Lonicerae alcohol extracts, stirring and dissolving;
(4) merge the solution that above-mentioned (2) and (3) step make, stir, keep 15～20 minutes, add 2 parts of described menthols, 18～43 parts of distilled water after medicinal liquid is clear and bright, the filtration that stirs.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011101982511A CN102274265B (en) | 2011-07-15 | 2011-07-15 | Compound propolis composition for treating livestock mastitis and preparation method thereof |
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| CN2011101982511A CN102274265B (en) | 2011-07-15 | 2011-07-15 | Compound propolis composition for treating livestock mastitis and preparation method thereof |
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| CN102274265B true CN102274265B (en) | 2013-12-04 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102973766A (en) * | 2012-11-22 | 2013-03-20 | 青岛绿曼生物工程有限公司 | Compound spina gleditsiae composition for treating mastitis in livestock, and preparation method thereof |
| CN105998323A (en) * | 2016-05-27 | 2016-10-12 | 青岛绿曼生物工程有限公司 | Film-forming pharmaceutical composition for preventing and treating cow mastitis as well as application and preparation method of pharmaceutical composition |
| CN108478616A (en) * | 2018-06-29 | 2018-09-04 | 佛山市南海东方澳龙制药有限公司 | Compound ketoprofen medicinal composition, pharmaceutical preparation and its application |
| RU2706689C1 (en) * | 2019-07-09 | 2019-11-20 | федеральное государственное бюджетное научное учреждение "Федеральный центр токсикологической, радиационной и биологической безопасности" (ФГБНУ "ФЦТРБ-ВНИВИ") | Method for treating mastitis in cows |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101537104A (en) * | 2009-05-07 | 2009-09-23 | 赵守财 | Traditional Chinese medicinal composition for treating dairy cow mastitis, preparation method and applications thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101537104A (en) * | 2009-05-07 | 2009-09-23 | 赵守财 | Traditional Chinese medicinal composition for treating dairy cow mastitis, preparation method and applications thereof |
Non-Patent Citations (2)
| Title |
|---|
| 蜂胶的现代药理研究;郭芳彬;《养蜂科技》;20021021(第05期);21－25 * |
| 郭芳彬.蜂胶的现代药理研究.《养蜂科技》.2002,(第05期), |
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