CN102273548A - Feeding microecological preparation and preparation method thereof - Google Patents
Feeding microecological preparation and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 63
- 240000007817 Olea europaea Species 0.000 claims abstract description 19
- 235000002725 Olea europaea Nutrition 0.000 claims abstract description 19
- 239000010902 straw Substances 0.000 claims abstract description 15
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- 244000005700 microbiome Species 0.000 claims abstract description 9
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- 241000894006 Bacteria Species 0.000 claims description 38
- 239000007788 liquid Substances 0.000 claims description 38
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 28
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 28
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 25
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 25
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- 238000011081 inoculation Methods 0.000 claims description 15
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a feeding microecological preparation. The feeding microecological preparation is prepared by a method comprising the following steps of: mixing straws, bran and urea, inoculating microorganisms and fermenting, adding fructus monordicae residue and olive leaf residue into the obtained product, mixing, and continuing to ferment. The effective viable count in a feeding microecological preparation product is 8*10<9> to 8*10<11> CFU/g, and the quality guarantee period is over 14 months.
Description
Technical field
The present invention relates to the fermentation field, in particular to a kind of feeding micro-ecological preparation.
Background technology
Feeding micro-ecological preparation is the probiotics that directly adds use as feed addictive.It can not only promote the foundation of animal gastrointestinal tract normal flora, strengthens immunity of livestock, and the generation that reduces disease promotes growth, improves the livestock and poultry daily gain, improves the quality of livestock products, can also produce several amino acids, vitamin and some unknown short living beings; Can also stimulate immunologic function in the enteron aisle, in time kill the invasion germ, the generation that can reduce ammonia and other corrupt substance again hinders the absorption of harmful substance and refuse.
China Ministry of Agriculture in 1999 has announced 12 kinds of the feed level microbe additive bacterial classifications of direct feeding animals, i.e. Lactobacillus casei, Lactobacillus plantarum, lactobacillus acidophilus, streptococcus fecalis, streptococcus lactis, bacillus subtilis, bafillus natto, lactic acid sheet ball, swamp Rhodopseudomonas, brewer's yeast, candida utili, aspergillus for No. 105.The feeding micro-ecological preparation that at present domestic and international ruminant is generally used has lactic acid bacteria class Bacillus acidi lactici, Bifidobacterium etc., bacillus category bacillus subtilis, bacillus cereus or the like bacterium class, aspergillus class aspergillus niger, aspergillus oryzae, yeast type beer yeast and oil yeast and culture and single bacteria preparation of Mycophyta and composite bacteria preparation.
The utilization of middle pharmaceutically active ingredient makes that the Chinese medicine level of residue is big, and environmental pollution is serious, to its effective utilization, can turn waste into wealth.Probiotics is an active bacteria formulation, effectively amount of viable bacteria and to keep be the Chinese medicine basis of such product quality.
Momordica grosvenori originates in Guangxi of China Guilin, belongs to Curcurbitaceae liana deciduous plant, and dioecism has annual kind piece root shape perennial root for many years.Generally early spring temperature be stabilized in 12 ℃ of germinations, the genus indefinite inflorescence of blooming after May, general 65 days maturations behind the setting.Fruits nutrition is worth very high, dry fruit total sugar content 25.17-38.31%, the sweet glucoside sweeter 300 times than cane suger particularly arranged, fresh fruit also contains abundant vitamin C, protein and amino acid, fruit distinguish the flavor of sweet cool in nature, moistening lung, heat-clearing arranged, relieve summer heat, promote the production of body fluid, the effect of cough-relieving, can treat obesity, diabetes, bronchitis, tonsillitis, sphagitis, acute gastritis, asthma etc., simultaneously, also be the good fruit of beverage and condiment.Its piece root can also be cured sore furuncle and nameless sores or boils.Lo Han Guo fruit and Ye Jun contain mogroside, and its glucoside unit belongs to triterpenes, tool intense sweetness (than sweet approximately 300 times of sucrose), and content reaches 22% in the fruit.Other contains a large amount of fructose.This product also contains ten several amino acids by analysis, and its total content pericarp is 3.51%, and seed is 8.47%, and planting benevolence is 37.82%.Flavones is still arranged in addition.Water is carried Momordica grosvenori Chinese medicine residue and is not carried and can participate in harmful substance, can utilize part active material wherein that microorganism is acted on.Contain multiple effective ingredient such as farnesol, fatty aldehyde and dietary fiber in the Momordica grosvenori residue.
Mainly contain compositions such as secoiridoid and glycosides, flavones and glycosides thereof, biflavone and glycosides thereof, low branch Chinese tannin in the olive leaf, the secoiridoid class is a main active.Olive leaf P.E has pure effect to having communicable and pernicious microorganism.It can stop as flu and other viral disease, fungi, the invasion of mould and saccharomycete, the outbreak of slight and severe bacterial infections and primary class parasitic infection, and be not only preventative, Olive leaf P.E provide a kind of with the fight of microorganism in safe and efficient methods of treatment.Research proves that also this extract only attacks pathogen and harmless to the enteric bacteria of human body symbiosis, and this is that it surmounts artificial antibiotic another advantage.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method of feeding micro-ecological preparation and the feeding micro-ecological preparation that obtains by this method, preparation method of the present invention comprises step: straw, wheat bran and urea are mixed, microbe inoculation ferments then, again Momordica grosvenori residue and olive leaf residue are added on and proceed fermentation after wherein mixing, promptly.
Described mixing comprises: earlier straw is pulverized, press the urea of straw dry weight interpolation 0.4-8% and the wheat bran of 30-40% then, adding water, to make the mixture moisture content be 55-65%, and boiling 60-90min under normal pressure is cooled to 55-60 ℃ then.
The microorganism of described inoculation is the liquid mixed culture fermentation agent of candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus.
Preferably, described candida utili is candida utili ACC20060, and described saccharomyces cerevisiae is saccharomyces cerevisiae ACC20065, and described Lactobacillus plantarum is Lactobacillus plantarum ACC10644, and described lactobacillus acidophilus is lactobacillus acidophilus ACC10637.Four kinds of bacterial classifications all can be available from Chinese agriculture microorganism fungus kind preservation center.
The inoculum concentration of described microorganism is 0.5-1%.
Described fermentation was fermented 64-72 hour for preserving moisture at 30-35 ℃.
The addition of described Momordica grosvenori residue and olive leaf residue is the 30-40% of the tunning quality that obtains of microbe inoculation, and the mass ratio of described Momordica grosvenori residue and olive leaf residue is 1:1-3.
Adding the continuation fermentation of carrying out behind Momordica grosvenori residue and the olive leaf residue ferments for carrying out air blast at 40-50 ℃.
The present invention also provides the feeding micro-ecological preparation that is obtained by above-mentioned preparation method.
The living bacteria count of described probiotics is 8 * 10
9CFU/g-8 * 10
11CFU/g.
The preparation method of the liquid mixed culture fermentation agent of candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus is as follows among the present invention:
The bacterial classification production method:
Saccharomycete slant strains and activated spawn: saccharomycete is seeded on the PDA inclined-plane cultivates, long being put in after good preserved in the refrigerator, or is seeded in and cultivates in the liquid triangular flask that to make ripe saccharomycete bacterial classification standby;
The preparation of lactic acid bacteria culturers: with the activated bevel bacterial classification of the former bacterium of lactic acid bacteria, it is standby to cultivate the lactobacillus inoculation of making maturation through the liquid triangular flask again;
Produce preparation with bacterial classification
Its production is used bacterial classification according to the method known to the those skilled in the art by producing required bacterial classification liquid measure making production with the production method of bacterial classification, and its production method can be consulted the preparation method of general microbe industrial fermentation bacterial classification.
More specifically, the preparation method of the liquid mixed culture fermentation agent of candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus is as follows among the present invention:
Respectively above-mentioned four kinds of bacterial classifications are activated, candida utili, saccharomyces cerevisiae are inoculated in respectively on the PDA slant medium, 30-35 ℃, cultivate 30-40h; After the activation, cultivate respectively and produce candida utili, saccharomyces cerevisiae liquid seeds continuously; And Lactobacillus plantarum, lactobacillus acidophilus are inoculated in the MRS slant medium respectively, 35-40 ℃, cultivate 30-40h; After the activation, cultivate respectively and produce Lactobacillus plantarum, lactobacillus acidophilus liquid seeds continuously.
With above-mentioned saccharomyces cerevisiae that makes and candida utili combined inoculation in the YPD seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 30-35 ℃, 160-180r/min, cultivate 20-30h, obtain the saccharomycete seed mixture, and then be inoculated in the saccharomycete seed mixture culture medium in 26-30 ℃ 160-180r/min, cultivate 20-30h, get saccharomycete seed mixture liquid.Being prepared as of saccharomycete seed mixture culture medium wherein: peptone 10.0g, glucose 20.0g, yeast extract 5.0g, distilled water 1000mL, adjust pH are 6.8-7.0, sterilization treatment is stand-by.
With Lactobacillus plantarum, lactobacillus acidophilus combined inoculation in the MRS seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 35-40 ℃, cultivate 20-30h, obtain the lactic acid bacteria seed mixture, and then be inoculated in the lactic acid bacteria seed mixture culture medium under 30-34 ℃, cultivate 20-30h, get lactic acid bacteria seed mixture liquid.Wherein lactic acid bacteria seed mixture medium preparation is: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, epsom salt 0.58g, four water manganese sulfate 0.25g, water 1000mL, adjust pH 6.2-6.4, sterilization treatment, stand-by.
Be 1 with lactic acid bacteria seed mixture liquid according to quantity ratio between the bacterial classification with the above-mentioned saccharomycete seed mixture liquid that makes then: 1-1: 3 mass ratio mixes, make candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus the liquid mixed culture fermentation agent.
The present invention has following beneficial effect:
The material that uses in the feeding micro-ecological preparation product of the present invention is harmless; with through the straw behind the microbial action and wheat bran as carrier; add of the protective effect of specific Chinese medicine residue, prolong the shelf-life of this series products, meet the development trend of feed as the probiotics viable bacteria.Wherein living bacteria count is 8 * 10
9More than the CFU/g, the shelf-life is more than 14 months.
The feeding micro-ecological preparation that the present invention makes can not only promote the foundation of animal gastrointestinal tract normal flora, strengthens immunity of livestock, and the generation that reduces disease promotes growth, improves the livestock and poultry daily gain.And the material that feeding micro-ecological preparation provided by the invention is selected for use in the preparation is harmless; with through the straw behind the microbial action and wheat bran as carrier; add of the protective effect of specific Chinese medicine residue as the probiotics viable bacteria; prolong the shelf-life of this series products; can extend to 18 months at most; compare with existing feeding micro-ecological preparation, prolonged the shelf-life greatly, meet the development trend of feed.
The specific embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Momordica grosvenori residue that adopts among the embodiment and olive leaf residue are all available from Changsha oceangoing voyage biological products Co., Ltd.
The preparation of embodiment 1 feeding micro-ecological preparation
Actication of culture
Candida utili, saccharomyces cerevisiae are inoculated in respectively on the PDA slant medium, 30 ℃, cultivate 30h; After the activation, cultivate respectively and produce candida utili, saccharomyces cerevisiae liquid seeds continuously; And Lactobacillus plantarum, lactobacillus acidophilus are inoculated in the MRS slant medium respectively, 35 ℃, cultivate 30h; After the activation, cultivate respectively and produce Lactobacillus plantarum, lactobacillus acidophilus liquid seeds continuously.
The seed liquor preparation
With above-mentioned saccharomyces cerevisiae that makes and candida utili combined inoculation in the YPD seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 30 ℃, 160r/min, cultivate 20h, obtain the saccharomycete seed mixture, and then be inoculated in the saccharomycete seed mixture culture medium in 26 ℃ 160r/min, cultivate 20h, get saccharomycete seed mixture liquid.Being prepared as of saccharomycete seed mixture culture medium wherein: peptone 10.0g, glucose 20.0g, yeast extract 5.0g, distilled water 1000mL, adjust pH are 6.8, and be at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
With Lactobacillus plantarum, lactobacillus acidophilus combined inoculation in the MRS seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 35 ℃, cultivate 20h, obtain the lactic acid bacteria seed mixture, and then be inoculated in the lactic acid bacteria seed mixture culture medium under 30 ℃, cultivate 20h, get lactic acid bacteria seed mixture liquid.Wherein lactic acid bacteria seed mixture medium preparation is: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, epsom salt 0.58g, four water manganese sulfate 0.25g, water 1000mL, adjust pH 6.2, at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
The preparation process of feeding micro-ecological preparation is as follows:
1) be that 1: 1 mass ratio mixes with the above-mentioned saccharomycete seed mixture liquid that makes and lactic acid bacteria seed mixture liquid according to quantity ratio between the bacterial classification, make candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus the liquid mixed culture fermentation agent.
2) raw material is carried out preliminary treatment, promptly earlier straw is pulverized, press the urea of straw dry weight interpolation 0.4% and 30% wheat bran then, add water-wet, making the mixture moisture content is 55%, boiling 60min under normal pressure then, to be cooled during to 55 ℃ of left and right sides, inoculate the 0.5% above-mentioned liquid mixed culture fermentation agent that makes, 30 ℃ of fermentations 64 hours of preserving moisture, 30% interpolation Momordica grosvenori residue and olive leaf residue (ratio of Momordica grosvenori residue and olive leaf residue is 1: 1) in this tunning quality mixes then, ferments up to obtaining dry products 40 ℃ of air blast.The product living bacteria count is 8 * 10
9CFU/g, the shelf-life is 14 months.
The preparation of embodiment 2 feeding micro-ecological preparations
Actication of culture
Candida utili, saccharomyces cerevisiae are inoculated in respectively on the PDA slant medium, 35 ℃, cultivate 40h; After the activation, cultivate respectively and produce candida utili, saccharomyces cerevisiae liquid seeds continuously; And Lactobacillus plantarum, lactobacillus acidophilus are inoculated in the MRS slant medium respectively, 40 ℃, cultivate 40h; After the activation, cultivate respectively and produce Lactobacillus plantarum, lactobacillus acidophilus liquid seeds continuously.
With above-mentioned saccharomyces cerevisiae that makes and candida utili combined inoculation in the YPD seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 35 ℃, 180r/min, cultivate 30h, obtain the saccharomycete seed mixture, and then be inoculated in the saccharomycete seed mixture culture medium in 30 ℃ 180r/min, cultivate 30h, get saccharomycete seed mixture liquid.Being prepared as of saccharomycete seed mixture culture medium wherein: peptone 10.0g, glucose 20.0g, yeast extract 5.0g, distilled water 1000mL, adjust pH are 7.0, and be at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
With Lactobacillus plantarum, the lactobacillus acidophilus combined inoculation is (quantitative proportion between the bacterial classification is 1: 1) in the MRS seed culture medium, under 40 ℃, cultivate 30h, obtain the lactic acid bacteria seed mixture, and then be inoculated in the lactic acid bacteria seed mixture culture medium under 34 ℃, cultivate 30h, get lactic acid bacteria seed mixture liquid, wherein lactic acid bacteria seed mixture medium preparation is: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, epsom salt 0.58g, four water manganese sulfate 0.25g, water 1000mL, adjust pH 6.4, at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
The preparation process of feeding micro-ecological preparation is as follows:
1) be that 1: 3 mass ratio mixes with the above-mentioned saccharomycete seed mixture liquid that makes and lactic acid bacteria seed mixture liquid according to quantity ratio between the bacterial classification, make candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus the liquid mixed culture fermentation agent.
2) raw material is carried out preliminary treatment, promptly earlier straw is pulverized, press the urea of straw dry weight interpolation 8% and 40% wheat bran then, add water-wet, making the mixture moisture content is 65%, boiling 90min under normal pressure then, to be cooled during to 65 ℃ of left and right sides, inoculate the 1% above-mentioned liquid mixed culture fermentation agent that makes, 35 ℃ of fermentations 72 hours of preserving moisture, 40% interpolation Momordica grosvenori residue and olive leaf residue (ratio of Momordica grosvenori residue and olive leaf residue is 1: 3) in this tunning quality mixes then, ferments up to obtaining dry products 40 ℃ of air blast.The product living bacteria count is 8 * 10
11CFU/g, the shelf-life is 15 months.
The preparation of embodiment 3 feeding micro-ecological preparations
Actication of culture
Candida utili, saccharomyces cerevisiae are inoculated in respectively on the PDA slant medium, 32 ℃, cultivate 35h; After the activation, cultivate respectively and produce candida utili, saccharomyces cerevisiae liquid seeds continuously; And Lactobacillus plantarum, lactobacillus acidophilus are inoculated in the MRS slant medium respectively, 37 ℃, cultivate 35h; After the activation, cultivate respectively and produce Lactobacillus plantarum, lactobacillus acidophilus liquid seeds continuously.
With above-mentioned saccharomyces cerevisiae that makes and candida utili combined inoculation in the YPD seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 33 ℃, 170r/min, cultivate 25h, obtain the saccharomycete seed mixture, and then be inoculated in the saccharomycete seed mixture culture medium in 28 ℃ 170r/min, cultivate 25h, get saccharomycete seed mixture liquid.Being prepared as of saccharomycete seed mixture culture medium wherein: peptone 10.0g, glucose 20.0g, yeast extract 5.0g, distilled water 1000mL, adjust pH are 6.9, and be at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
With Lactobacillus plantarum, lactobacillus acidophilus combined inoculation in the MRS seed culture medium (quantitative proportion between the bacterial classification is 1: 1), under 37 ℃, cultivate 25h, obtain the lactic acid bacteria seed mixture, and then be inoculated in the lactic acid bacteria seed mixture culture medium under 32 ℃, cultivate 25h, get lactic acid bacteria seed mixture liquid.Wherein lactic acid bacteria seed mixture medium preparation is: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, epsom salt 0.58g, four water manganese sulfate 0.25g, water 1000mL, adjust pH 6.3, at 115 ℃ of following high pressure moist heat sterilizations, stand-by.
The preparation process of feeding micro-ecological preparation is as follows:
1) be that 1: 2 mass ratio mixes with the above-mentioned saccharomycete seed mixture liquid that makes and lactic acid bacteria seed mixture liquid according to quantity ratio between the bacterial classification, make candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus the liquid mixed culture fermentation agent.
2) raw material is carried out preliminary treatment, promptly earlier straw is pulverized, press the urea of straw dry weight interpolation 6% and 35% wheat bran then, add water-wet, making the mixture moisture content is 60%, boiling 70min under normal pressure then, to be cooled during to 60 ℃ of left and right sides, inoculate the 0.8% above-mentioned liquid mixed culture fermentation agent that makes, 32 ℃ of fermentations 68 hours of preserving moisture, 45% interpolation Momordica grosvenori residue and olive leaf residue (ratio of Momordica grosvenori residue and olive leaf residue is 1: 2) in this tunning quality mixes then, ferments up to obtaining dry products 45 ℃ of air blast.The product living bacteria count is 8 * 10
11CFU/g, the shelf-life is 18 months.
The shelf-life that has commercially available feeding micro-ecological preparation now was generally about 6 months, the feeding micro-ecological preparation that the present invention prepares not only strengthens immunity of livestock, the generation that reduces disease promotes growth, improve the livestock and poultry daily gain, also prolonged the shelf-life of this series products, can extend to 18 months at most, compared with existing feeding micro-ecological preparation, prolong the shelf-life greatly, met the development trend of feed.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, this modification of this that made or improvement without departing from theon the basis of the spirit of the present invention all belongs to the scope of protection of present invention.
Claims (10)
1. the preparation method of a feeding micro-ecological preparation, it comprises the steps: straw, wheat bran and urea are mixed, microbe inoculation ferments then, Momordica grosvenori residue and olive leaf residue is added on to proceed fermentation after wherein mixing again, promptly.
2. the preparation method of feeding micro-ecological preparation according to claim 1, it is characterized in that, described mixing comprises: earlier straw is pulverized, press the urea of straw dry weight interpolation 0.4-8% and the wheat bran of 30-40% then, adding water, to make the mixture moisture content be 55-65%, boiling 60-90min under normal pressure is cooled to 55-60 ℃ then.
3. the preparation method of feeding micro-ecological preparation according to claim 1 is characterized in that, the microorganism of described inoculation is the liquid mixed culture fermentation agent of candida utili, saccharomyces cerevisiae, Lactobacillus plantarum and lactobacillus acidophilus.
4. the preparation method of feeding micro-ecological preparation according to claim 3, it is characterized in that, described candida utili is candida utili ACC20060, described saccharomyces cerevisiae is saccharomyces cerevisiae ACC20065, described Lactobacillus plantarum is Lactobacillus plantarum ACC10644, and described lactobacillus acidophilus is lactobacillus acidophilus ACC10637.
5. according to the preparation method of claim 1 or 3 described feeding micro-ecological preparations, it is characterized in that the inoculum concentration of described microorganism is 0.5-1%.
6. the preparation method of feeding micro-ecological preparation according to claim 1 is characterized in that, described fermentation was fermented 64-72 hour for preserving moisture at 30-35 ℃.
7. the preparation method of feeding micro-ecological preparation according to claim 1, it is characterized in that, the addition of described Momordica grosvenori residue and olive leaf residue is the 30-40% of the tunning quality that obtains of microbe inoculation, and the mass ratio of described Momordica grosvenori residue and olive leaf residue is 1: 1-3.
8. the preparation method of feeding micro-ecological preparation according to claim 1 is characterized in that, adds the fermentation of carrying out behind Momordica grosvenori residue and the olive leaf residue and ferments for carrying out air blast at 40-50 ℃.
9. the feeding micro-ecological preparation that obtains according to the described preparation method of claim 1-8.
10. feeding micro-ecological preparation according to claim 9 is characterized in that the living bacteria count of described probiotics is 8 * 10
9CFU/g-8 * 10
11CFU/g.
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| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
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| CN102696865A (en) * | 2012-05-18 | 2012-10-03 | 河南科技大学 | Method for producing probiotic bacteria granular feed by fermenting corn straw with mixed strain |
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| CN103798504A (en) * | 2014-01-03 | 2014-05-21 | 中国林业科学研究院林产化学工业研究所 | Method for preparing biological feed by solid fermenting olive leaves |
| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
| CN104171325A (en) * | 2014-07-29 | 2014-12-03 | 张释文 | Animal feed |
| CN105647830A (en) * | 2016-02-29 | 2016-06-08 | 海南泓缘生物科技股份有限公司 | Composite microbial agent for enhancing animal immunity, preparation method and application of composite microbial agent |
| CN113016714A (en) * | 2021-03-04 | 2021-06-25 | 湖南中医药大学 | Preparation method of animal model with diarrhea and dyspepsia gastrointestinal syndrome and method for evaluating animal model with diarrhea and dyspepsia gastrointestinal syndrome |
| CN113016714B (en) * | 2021-03-04 | 2023-08-22 | 湖南中医药大学 | Preparation method of animal model with diarrhea and food stagnation gastrointestinal symptoms |
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