CN102245774A - 使用含有突变rxxd基序的二鸟苷酸环化酶酶促生产环二鸟苷单磷酸的方法 - Google Patents

使用含有突变rxxd基序的二鸟苷酸环化酶酶促生产环二鸟苷单磷酸的方法 Download PDF

Info

Publication number
CN102245774A
CN102245774A CN2009801489188A CN200980148918A CN102245774A CN 102245774 A CN102245774 A CN 102245774A CN 2009801489188 A CN2009801489188 A CN 2009801489188A CN 200980148918 A CN200980148918 A CN 200980148918A CN 102245774 A CN102245774 A CN 102245774A
Authority
CN
China
Prior art keywords
dgc
gmp
gtp
sudden change
motif
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801489188A
Other languages
English (en)
Chinese (zh)
Inventor
T·S·埃尔葛
V·斯佩尔
R·瓦拉斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intervet International BV
Original Assignee
Intervet International BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intervet International BV filed Critical Intervet International BV
Publication of CN102245774A publication Critical patent/CN102245774A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C07H19/207—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2009801489188A 2008-12-09 2009-12-07 使用含有突变rxxd基序的二鸟苷酸环化酶酶促生产环二鸟苷单磷酸的方法 Pending CN102245774A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12092508P 2008-12-09 2008-12-09
EP08171026 2008-12-09
US61/120,925 2008-12-09
EP08171026.1 2008-12-09
PCT/EP2009/066492 WO2010066666A1 (en) 2008-12-09 2009-12-07 Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated rxxd motif

Publications (1)

Publication Number Publication Date
CN102245774A true CN102245774A (zh) 2011-11-16

Family

ID=40602165

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801489188A Pending CN102245774A (zh) 2008-12-09 2009-12-07 使用含有突变rxxd基序的二鸟苷酸环化酶酶促生产环二鸟苷单磷酸的方法

Country Status (12)

Country Link
US (1) US20110288286A1 (enExample)
EP (1) EP2376634A1 (enExample)
JP (1) JP2012511313A (enExample)
KR (1) KR20110099111A (enExample)
CN (1) CN102245774A (enExample)
AR (1) AR074495A1 (enExample)
AU (1) AU2009326306B2 (enExample)
BR (1) BRPI0923296A2 (enExample)
CA (1) CA2747127A1 (enExample)
TW (1) TW201033367A (enExample)
UY (1) UY32307A (enExample)
WO (1) WO2010066666A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113122593A (zh) * 2019-12-31 2021-07-16 安徽古特生物科技有限公司 一种利用多聚磷酸盐制备核苷三磷酸和脱氧核苷三磷酸的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6047143B2 (ja) 2012-02-29 2016-12-21 ヤマサ醤油株式会社 ｃｙｃｌｉｃｄｉ−ＧＭＰの実践的酵素合成法
CN111647611A (zh) * 2020-07-02 2020-09-11 杭州美亚药业股份有限公司 一种双鸟苷酸环化酶改造基因、编码蛋白、工程菌及其构建方法和应用
CN117701596B (zh) * 2023-12-08 2026-07-03 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种大量合成天然环二鸟苷酸的基因工程菌及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
JP3995279B2 (ja) * 1994-09-07 2007-10-24 アスビオファーマ株式会社 蛋白の製造方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHRISTEN B.等: "Allosteric control of cyclic di-GMP signaling", 《JOURNAL OF BIOLOGICAL CHEMISTRY》 *
郭井利等: "重组蛋白在大肠杆菌中包涵体蛋白的表达及其复性", 《畜牧兽医科技信息》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113122593A (zh) * 2019-12-31 2021-07-16 安徽古特生物科技有限公司 一种利用多聚磷酸盐制备核苷三磷酸和脱氧核苷三磷酸的方法

Also Published As

Publication number Publication date
KR20110099111A (ko) 2011-09-06
WO2010066666A1 (en) 2010-06-17
US20110288286A1 (en) 2011-11-24
CA2747127A1 (en) 2010-06-17
UY32307A (es) 2010-02-26
BRPI0923296A2 (pt) 2015-08-11
AU2009326306A1 (en) 2010-06-17
TW201033367A (en) 2010-09-16
JP2012511313A (ja) 2012-05-24
AR074495A1 (es) 2011-01-19
EP2376634A1 (en) 2011-10-19
AU2009326306B2 (en) 2012-12-13

Similar Documents

Publication Publication Date Title
JPS63233798A (ja) ５′−グアニル酸の製造法
AU2018344883B2 (en) Cellular transport system for transferring a sulfonic acid construct carrying a cargo into the cytoplasm of a cell
EP3976809A1 (en) Variants of terminal deoxynucleotidyl transferase and uses thereof
JP6638086B2 (ja) フルクトースからアロースを生産する菌株およびこれを用いたアロース生産方法
CN112673100B (zh) 包括非标准氨基酸的组合物及其用途
AU2009326306B2 (en) Process for the enzymatic production of cyclic diguanosine monophosphate employing a diguanylate cyclase comprising a mutated RXXD motif
EP2094840B1 (en) Novel n-acetylglucosamine-2-epimerase and method for producing cmp-neuraminic acid using the same
WO2024082546A1 (zh) 阿糖类核苷一锅法生物合成的方法及组合物
CN107083412B (zh) 一种中短链酰基辅酶a合成酶的应用
CN116855467A (zh) 一种化学-酶偶联方法用于合成麦角硫因
KR20140133744A (ko) SepRS와 EF-Tu의 변이체를 이용한 인산화 단백질의 제조방법
CN113403287A (zh) 分离的多肽、核酸及其应用
JP3822006B2 (ja) シチジン５’−ジリン酸コリンの製造法
WO2003008619A1 (en) Transglycosylation of nucleosides by biocatalysis with immobilized and stabilized enzymes.
KR100942133B1 (ko) 신규 뉴클레오시드 하이드롤라제 ⅱ
KR100941304B1 (ko) 신규 뉴클레오시드 하이드롤라제ⅰ
TW202519654A (zh) 解氨酶及其用途
CN120555536A (zh) 一种酶法合成ppGpp的方法
CN115975987A (zh) 一种AscI限制性内切酶的制备方法
CN119552932A (zh) 胞苷-5’-二磷酸的制备方法
KR101223666B1 (ko) 나이트릴레이즈 ｏｒｎ을 이용한 카르복실산의 제조방법
KR101223663B1 (ko) 나이트릴레이즈 ｖｍｎ１을 이용한 카르복실산의 제조방법
KR101223665B1 (ko) 나이트릴레이즈 ｒｍｎ２을 이용한 카르복실산의 제조방법
KR101223664B1 (ko) 나이트릴레이즈 ｒｍｎ１을 이용한 카르복실산의 제조방법
CN115710594A (zh) 一种合成尿苷二磷酸-6-叠氮-d-半乳糖的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20111116