CN102232363B - Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely - Google Patents
Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely Download PDFInfo
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- CN102232363B CN102232363B CN2010101695565A CN201010169556A CN102232363B CN 102232363 B CN102232363 B CN 102232363B CN 2010101695565 A CN2010101695565 A CN 2010101695565A CN 201010169556 A CN201010169556 A CN 201010169556A CN 102232363 B CN102232363 B CN 102232363B
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for artificially inducing sexual glands of Anguilla marmorata to develop maturely. The method comprises the following steps of: accelerating the maturation of male Anguilla marmorata by intramuscularly injecting human chorionic gonadotropin (HCG), wherein the dosage is 500U/kg based on the body weight of the male Anguilla marmorata, the HCG is injected once every week, and the injection time is 6 weeks; and accelerating the maturation of female Anguilla marmorata by intramuscularly injecting luteinizing hormone releasing hormone A2 (LHRH-A2) and carp pituitaryextract (CPE) homogenate, the dosage of the LHRH-A2 is 250mu g/kg based on the body weight of the female Anguilla marmorata, the dosage of CPE is 4 grains/kg based on the body weight of the female Anguilla marmorata, the LHRH-A2 and the CPE are injected once every 7 to 9 days, and the injection times are 15 to 18. By the scheme, sexual glands of parent fish are developed maturely, abdomens are remarkably protuberant, and the sexual glands are remarkably in a spongy state when convex parts of the abdomens are touched by hands; the female Anguilla marmorata can release mature eggs; and milky white semens can be collected by extruding the abdomens of the male Anguilla marmorata. The method provides a preliminary technical assurance for further researching the completely artificial propagation of the Anguilla marmorata.
Description
Technical field
The invention belongs to aquaculture field, is the artificial propagation techniques of a kind of common eel, is specifically related to the method for hormone induction flower common eel gonadal maturation.
Background technology
Flower common eel (Anguilla marmorata) popular name perch eel, flower eel, snow eel, eel king, small stream eel etc., in taxonomy, belong to Osteichthyes (Osteichthyes), Anguilliforme (Anguilliformes), Anguillidae (Anguillidae), Anguilla (Anguilla), it is the widest kind that distributes in the Anguilla fish, distribution is to the east of all islands, Pacific middle part, Madagascar to the Indian Ocean, reach the Australia south in the south, north is to Korea, South Japan.Be mainly seen in the river and marine site of economizing in Hainan, Guangdong, Fujian, Taiwan etc. in China.The flower common eel be a kind of extra large breeding migration fish that fall, in the freshet lake, grow, after the sexual maturity migration thousands of to bank off sea in outside the spawning ground lay eggs.Under the nature, common eel is finished the growth of sexual gland in falling extra large breeding migration process, gonad maturity when arriving the spawning ground.
In recent years, build the mankind's activity aggravations such as dam owing to overfishing, environmental pollution, barrage, the natural resources of flower common eel obviously fails, and China has classified it as the second class protection animal.The flower common eel is delicious meat not only, is of high nutritive value, and medical value is arranged, and have the title of " fresh water ginseng ", is the high-quality edible fishes.At present, the ground develop actively flower common eels such as Hainan are propagated industry artificially, and market prospects are wide, and the industry development impetus is good, has become the famous and precious new varieties of freshwater aquiculture.Yet the artificial propagation techniques of Anguilla fish is still unresolved so far.Cultivation eel seedling all depends on fishes for wild eel seedling, causes the rapid decline of natural resources.
The artificial propagation techniques of common eel belongs to a global difficult problem, correlative study starts from eighties of last century forties, because the particularity of its history of life, Chinese scholars has been done a large amount of research work, existing artificial induction common eel method mainly relies on the multiple injection exogenous hormone, and has obtained interim success on Japanese eel.Yet the research that flower common eel artificial induced spawning accelerates the ripening at home and abroad is blank out, and this method is spent common eel to hasten parturition by the artificial induction and accelerated the ripening, and will lay the first stone for the artificial propagation of flower common eel.
Summary of the invention
The object of the present invention is to provide a kind of artificial induction to spend the method for common eel gonadal maturation, solve the flower common eel under Artificial feeding conditions, its sexual gland can't natural fully-developed problem.The method of colored common eel gonadal maturation of inducing provided by the present invention is as follows:
(1) raising and train of parent population: parent population is selected the above healthy artificial pool common eel of growing flower in 3 ages; The male flower common eel specification requirement of wherein selecting: body weight 0.425-0.875kg, the long 55-66cm of body; The instaminate flower common eel specification requirement of selecting: body weight 1.305-2.89kg, the long 73-99cm of body.Water temperature is controlled at 22-26 ℃ during raising and train; 24 hours lucifuges of whole day; Living bait and eel powder mix throws something and feeds every day 3 times; The parent population cultivation density is 1 tail/cylinder (specification is the glass jar of 100cm * 60cm * 50cm); The time of raising and train is 1 month.
(2) raise and train end after, stop to throw something and feed, the instaminate flower common eel needs excessively to begin to accelerate the ripening to half seawater, and the male flower common eel is accelerated the ripening in fresh water.
(3) induce gonadal maturation: the male flower common eel adopts intramuscular injection HCG to accelerate the ripening; The instaminate flower common eel is adopted intramuscular injection LHRH-A
2Homogenate is accelerated the ripening with carp brain hypophysis.
It is characterized in that:
(1) parent population is selected the above healthy pool common eel of growing flower in 3 ages; Water temperature is controlled at 22-26 ℃ during raising and train; 24 hours lucifuges of whole day; Living bait and eel powder mix throws something and feeds every day 3 times; The parent population cultivation density is 1 tail/cylinder; The time of raising and train is 1 month.
(2) instaminate flower common eel spawning inducing pool progressively increases salinity to half seawater, and every day, salinity improved 2-3, until till salinity is 14-17.
(3) the male flower common eel all adopts fresh water to cultivate during accelerating the ripening.
(4) the male flower common eel adopts intramuscular injection HCG to accelerate the ripening, and dosage is the 500U/kg body weight, and injection once injected for 6 weeks weekly; The instaminate flower common eel is adopted intramuscular injection LHRH-A
2LHRH-A is accelerated the ripening in homogenate with carp brain hypophysis (CPE)
2Dosage is 250 μ g/kg body weight, and the dosage of CPE is 4/kg body weight, and injection in every 7-9 days is once injected 15-18 time.
In inducing male flower common eel testis development maturation, respectively to the Contrast on effect that accelerates the ripening the middle male flower common eel of seawater and fresh water, the result shows that the spermary of two groups of fishes all reaches maturity, its sperm viability is all about 15%, rapid movement time and life-span do not have notable difference, illustrate that the male flower common eel is the same at seawater with the effect of accelerating the ripening in the fresh water.Therefore, carrying out in fresh water that the male flower common eel accelerates the ripening is an innovative point of patented technology of the present invention, can reduce so unnecessary man power and material's loss.
Adopt LHRH-A
2Inducing instaminate flower common eel gonadal maturation with the mixture of carp brain hypophysis homogenate is another innovative point of patented technology of the present invention, utilize gonadotropin releasing hormone analogue to induce colored common eel self to produce Endogenous Type gonadotropin, and cooperate external source gonadotropin (carp brain hypophysis) acting in conjunction, respond well.
By enforcement this programme, the parent population gonadal maturation, the obvious projection of belly is stroked the protruding place of the outside of belly with hand, feels that obviously sexual gland is soft flexible.In the 15 tail experimental group rauns that select arbitrarily, 5 tail development of ovary full maturitys, average GSI (sexual gland coefficient) is 20.9 ± 5.04% (maximum reaches 27.3%); The 5 tail developments of ovary are to the maturing stage, and average GSI is 7.2 ± 2.86%; The 5 tail developments of ovary are to the yolk generation, and average GSI is 2.32 ± 1.5%; And the sexual gland of control group rests on the rudimentary II phase (GSI is 1.18 ± 0.39%), and anatomy experiment group raun artificial induced spawning accelerates the ripening to observe and finds, visible a large amount of ovums on the ovary.And male flower common eel GSI (sexual gland coefficient) reaches 9.5 ± 2.1%, and the extruding belly can be collected the milky seminal fluid.
Description of drawings
Fig. 1: control group male flower common eel belly solution figure
Fig. 2: experimental group male flower common eel belly solution figure
Fig. 3: control group instaminate flower common eel belly solution figure
Fig. 4: experimental group instaminate flower common eel belly solution figure
Embodiment
Following implementation method is a concrete application example of the present invention, and is not limited only to this example.
(1) the foster common eel in the artificial pool that will collect is put into glass jar by the density of 1 tail/cylinder and raises and train, and adopts indoor circulating water cultivation system, 24 hours lucifuges of whole day, and water temperature is controlled at 22-26 ℃.The time of raising and train is 1 month, and the mixing of living bait and eel powder is thrown something and fed during raising and train, and every day 3 times, will eat surplus feed after throwing something and feeding 3 hours at every turn and clean out.
(2) parent population enters half seawater from fresh water need to through the whole process of osmotic pressure and physiology adjusting, need certain hour.The instaminate flower common eel salinity conditioning period bait of not throwing something and feeding.Rose in the 2nd day that stops to throw something and feed, every day, salinity improved 2-3, until salinity is brought up to till the 14-17, lasted 7-9 days, if observe parent population uncomfortable performance was arranged, and stopped immediately improving salinity, continued to increase salinity until reach till the requirement after waiting parent population in stable condition again.
(3) begin the experiment of accelerating the ripening of flower common eel behind transition half seawater.The male flower common eel process of accelerating the ripening: parent population is pulled out from glass jar, put into mixture of ice and water anesthesia 10-20 minute; Use the towel that soaked frozen water to encase the parent population head after the anesthesia, with the HCG (dosage is the 500U/kg body weight) of fish physiological saline solution, syringe penetrates from the back intercostal, inject complete rear at pin hole place obliterating erythromycin ointment, to protect from infection, injection is once injected 6 times altogether weekly.The instaminate flower common eel process of accelerating the ripening: parent population is pulled out from glass jar, put into frozen water anesthesia after 10-20 minute, encase its head with the towel that soaked frozen water, with the LHRH-A of fish physiological saline solution
2With carp brain hypophysis (CPE) homogenate, adopt the intramuscular injection of 1mL syringe, LHRH-A
2Dosage is 250 μ g/kg body weight, and the dosage of CPE is 4 kg body weight, inject complete after at pin hole place obliterating erythromycin ointment, protecting from infection, injection in every 7-9 days is once injected 15-18 time altogether.
By enforcement this programme, the parent population gonadal maturation, the obvious projection of belly is stroked the protruding place of the outside of belly with hand, feels that obviously sexual gland is soft state.Dissect visible a large amount of ovums on the ovary of finding the instaminate flower common eel; The male flower common eel can be collected the milky seminal fluid by the extruding belly.This provides the technique guarantee in early stage for the whole artificial propagation of further studying the flower common eel.
Claims (4)
1. an artificial induction spends the method for common eel gonadal maturation, it is characterized in that, said method comprising the steps of:
(1) raising and train of parent population: parent population is selected the above healthy artificial pool common eel of growing flower in 3 ages; Water temperature is controlled at 22-26 ℃ during raising and train; 24 hours lucifuges of whole day; Living bait and eel powder mix throws something and feeds every day 3 times; The parent population cultivation density is 1 tail/cylinder; The time of raising and train is 1 month;
(2) raise and train end after, stop to throw something and feed, the instaminate flower common eel needs excessively to begin to accelerate the ripening to half seawater; And the male flower common eel is accelerated the ripening in fresh water;
(3) induce gonadal maturation: the male flower common eel adopts intramuscular injection HCG to accelerate the ripening; The instaminate flower common eel is adopted intramuscular injection LHRH-A
2Homogenate is accelerated the ripening with carp brain hypophysis.
2. spend the method for common eel gonadal maturation according to artificial induction claimed in claim 1, it is characterized in that: parent population is selected the pool common eel of growing flower; The male flower common eel specification requirement of wherein selecting: body weight 0.425-0.875kg, the long 55-66cm of body; The instaminate flower common eel specification requirement of selecting: body weight 1.305-2.89kg, the long 73-99cm of body; Water temperature is controlled at 22-26 ℃ during raising and train; 24 hours lucifuges of whole day; Living bait and eel powder mix throws something and feeds every day 3 times; The parent population cultivation density is 1 tail/cylinder; The time of raising and train is 1 month.
3. spend the method for common eel gonadal maturation according to artificial induction claimed in claim 1, it is characterized in that: progressively increase salinity to half seawater, every day, salinity improved 2-3, until salinity is adjusted to till the 14-17.
4. spend the method for common eel gonadal maturation according to artificial induction claimed in claim 1, it is characterized in that: the male flower common eel adopts intramuscular injection HCG to accelerate the ripening, and dosage is the 500U/kg body weight, injects once weekly, and be 6 weeks inject time; The instaminate flower common eel is adopted intramuscular injection LHRH-A
2LHRH-A is accelerated the ripening in homogenate with carp brain hypophysis CPE
2Dosage is 250 μ g/kg body weight, and the dosage of CPE is 4/kg body weight, injects once in every 7-9 days, and frequency injection is 15-18 time.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103911444B (en) * | 2014-03-19 | 2015-05-20 | 南京师范大学 | Primer for discriminating anguilla marmorata and anguilla bicolor pacifica fry and method thereof |
| CN105995036B (en) * | 2016-06-08 | 2019-05-07 | 集美大学 | Promote the additive and method of the Japanese eel development of ovary |
| CN110199922A (en) * | 2019-07-05 | 2019-09-06 | 深圳华大海洋科技有限公司 | A method of silver eel being changed by yellow eel for induction Female Japanese eel |
| CN114868676B (en) * | 2022-03-08 | 2022-11-11 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1099921A1 (en) * | 1982-03-02 | 1984-06-30 | Институт Зоологии Ан Бсср | Method of obtaining of sexual products from european eels |
| EP1686179A1 (en) * | 2005-01-26 | 2006-08-02 | Universiteit Leiden | Means and methods for improving the development and maturation of eggs and/or sperm in fish using hormones produced by transplanted cells |
| CN101461334A (en) * | 2007-12-17 | 2009-06-24 | 中国水产科学研究院东海水产研究所 | Method for obtaining normal mature sperm of eel |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1099921A1 (en) * | 1982-03-02 | 1984-06-30 | Институт Зоологии Ан Бсср | Method of obtaining of sexual products from european eels |
| EP1686179A1 (en) * | 2005-01-26 | 2006-08-02 | Universiteit Leiden | Means and methods for improving the development and maturation of eggs and/or sperm in fish using hormones produced by transplanted cells |
| CN101461334A (en) * | 2007-12-17 | 2009-06-24 | 中国水产科学研究院东海水产研究所 | Method for obtaining normal mature sperm of eel |
Non-Patent Citations (6)
| Title |
|---|
| Huang Hai et al.Molecular characterization of marbled eel (Anguilla marmorata) gonadotropin subunits and their mRNA expression profiles during artificially induced gonadal development.《General and Comparative Endocrinology》.2009,第162卷(第2期),全文. |
| Molecular characterization of marbled eel (Anguilla marmorata) gonadotropin subunits and their mRNA expression profiles during artificially induced gonadal development;Huang Hai et al;《General and Comparative Endocrinology》;20090630;第162卷(第2期);全文 * |
| 人工诱导花鳗鲡的精巢发育成熟及其精子的生物学特性;周雯伊等;《水产学报》;20090131;第33卷(第1期);全文 * |
| 周雯伊.激素诱导雄性花鳗鲡性腺发育及相关激素分泌的研究.《海南大学硕士学位论文》.2009,全文. |
| 周雯伊等.人工诱导花鳗鲡的精巢发育成熟及其精子的生物学特性.《水产学报》.2009,第33卷(第1期),全文. |
| 激素诱导雄性花鳗鲡性腺发育及相关激素分泌的研究;周雯伊;《海南大学硕士学位论文》;20090630;全文 * |
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