CN102209780B - 经改进的核黄素生产 - Google Patents

经改进的核黄素生产 Download PDF

Info

Publication number
CN102209780B
CN102209780B CN200980144726.XA CN200980144726A CN102209780B CN 102209780 B CN102209780 B CN 102209780B CN 200980144726 A CN200980144726 A CN 200980144726A CN 102209780 B CN102209780 B CN 102209780B
Authority
CN
China
Prior art keywords
riboflavin
rib
leader
modified
rib leader
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN200980144726.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN102209780A (zh
Inventor
马丁·莱玛恩
迈克尔·汉斯
汉斯-彼得·霍曼
迪特马尔·卢达尔特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CN102209780A publication Critical patent/CN102209780A/zh
Application granted granted Critical
Publication of CN102209780B publication Critical patent/CN102209780B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200980144726.XA 2008-11-07 2009-11-09 经改进的核黄素生产 Active CN102209780B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08019492A EP2186880A1 (en) 2008-11-07 2008-11-07 Improved production of riboflavin
EP08019492.1 2008-11-07
PCT/EP2009/064825 WO2010052319A1 (en) 2008-11-07 2009-11-09 Improved production of riboflavin

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201310713983.9A Division CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Publications (2)

Publication Number Publication Date
CN102209780A CN102209780A (zh) 2011-10-05
CN102209780B true CN102209780B (zh) 2016-02-10

Family

ID=40329198

Family Applications (2)

Application Number Title Priority Date Filing Date
CN200980144726.XA Active CN102209780B (zh) 2008-11-07 2009-11-09 经改进的核黄素生产
CN201310713983.9A Pending CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201310713983.9A Pending CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Country Status (7)

Country Link
US (1) US10633684B2 (enExample)
EP (2) EP2186880A1 (enExample)
JP (1) JP5787089B2 (enExample)
KR (1) KR101726886B1 (enExample)
CN (2) CN102209780B (enExample)
ES (1) ES2648045T3 (enExample)
WO (1) WO2010052319A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101335853B1 (ko) 2011-12-01 2013-12-02 씨제이제일제당 (주) Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법
DK3102669T3 (da) * 2014-02-07 2019-08-19 Dsm Ip Assets Bv Forbedret bacillevært
CN103865944B (zh) * 2014-02-20 2016-01-20 天津大学 一种生产核黄素的大肠杆菌菌株及构建方法及用途
KR102561864B1 (ko) * 2014-11-19 2023-08-01 바스프 에스이 Rib７ 프로모터를 사용하여 유전자 발현을 하향조절하기 위한 에레모테시움의 유전적 변형
CN107922464B (zh) * 2015-08-28 2022-03-15 帝斯曼知识产权资产管理有限公司 经改进的维生素生产
KR101844778B1 (ko) * 2017-01-18 2018-04-04 주식회사한국야쿠르트 비타민 b2 생합성능이 매우 우수한 락토바실러스 플란타룸 hy7715 및 이를 유효성분으로 함유하는 제품
CN108330136A (zh) * 2017-05-05 2018-07-27 北京航空航天大学 一种构建产黄素单核苷酸的工程菌株的方法及其应用
KR20200047668A (ko) * 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
CN111935981B (zh) 2018-04-05 2024-06-14 帝斯曼知识产权资产管理有限公司 变体麦芽糖α-淀粉酶
CN113015811A (zh) * 2018-11-15 2021-06-22 帝斯曼知识产权资产管理有限公司 改善的核黄素生产
WO2021260057A2 (en) 2020-06-23 2021-12-30 Dsm Ip Assets B.V. Fermentation process
CN114592000B (zh) * 2020-12-03 2023-07-21 上海市农业科学院 一种六基因组合在水稻种子中提高vb2含量的应用及方法
CN112538453B (zh) * 2020-12-15 2022-07-29 通辽梅花生物科技有限公司 一种产核黄素的枯草芽孢杆菌及其构建方法与应用
CN116463305B (zh) * 2023-06-15 2023-10-17 北京易醒生物科技有限公司 一种提高用于乙醇氧化的醇氧化酶表达量的方法、优化的核黄素生物合成基因
CN117660577B (zh) * 2023-12-08 2024-06-25 苏州华赛生物工程技术有限公司 LtaSA蛋白或其编码基因在核黄素生产中的应用
CN117568433B (zh) * 2024-01-15 2024-04-16 黑龙江新和成生物科技有限公司 一种提高枯草芽孢杆菌发酵生产核黄素的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1049185A (zh) * 1989-06-22 1991-02-13 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
US20030232406A1 (en) * 1989-06-22 2003-12-18 Roche Vitamins, Inc. Bacterial strains which overproduce riboflavin

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6656721B1 (en) 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
US20060127993A1 (en) 2003-06-02 2006-06-15 Goese Markus G Thiamin production by fermentation
KR101185656B1 (ko) 2003-06-18 2012-09-24 디에스엠 아이피 어셋츠 비.브이. 포자를 형성할 수 없는 미생물을 사용한 판토텐산염의 제조방법
JP5364376B2 (ja) 2005-11-02 2013-12-11 ディーエスエム アイピー アセッツ ビー．ブイ． 改変型トランスケトラーゼおよびその使用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1049185A (zh) * 1989-06-22 1991-02-13 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
US20030232406A1 (en) * 1989-06-22 2003-12-18 Roche Vitamins, Inc. Bacterial strains which overproduce riboflavin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Relationship between the secondary structure and the regulatory activity of the leader region of the riboflavin biosynthesis operon in Bacillus subtilis;Mironov, AS;《RUSSIAN JOURNAL OF GENETICS》;20080430;第44卷(第4期);399-404 *
强启动子枯草芽孢杆菌核黄素操纵子整合型载体的构建并在枯草芽孢杆菌中的表达;周世奇;《中国优秀硕士学位论文全文数据库》;20070515;全文 *

Also Published As

Publication number Publication date
EP2356211A1 (en) 2011-08-17
JP2012507984A (ja) 2012-04-05
CN103757000A (zh) 2014-04-30
US10633684B2 (en) 2020-04-28
WO2010052319A1 (en) 2010-05-14
US20110312025A1 (en) 2011-12-22
CN102209780A (zh) 2011-10-05
EP2356211B1 (en) 2017-09-20
KR20110093847A (ko) 2011-08-18
EP2186880A1 (en) 2010-05-19
KR101726886B1 (ko) 2017-04-13
JP5787089B2 (ja) 2015-09-30
ES2648045T3 (es) 2017-12-28

Similar Documents

Publication Publication Date Title
CN102209780B (zh) 经改进的核黄素生产
KR101558622B1 (ko) ｍＲＮＡ의 안정화를 통한 표적 생성물의 증가된 제조
CN107922464B (zh) 经改进的维生素生产
JP7497348B2 (ja) リボフラビンの改善された産生
US9315784B2 (en) Modified transketolase and use thereof
KR20200072543A (ko) 3-히드록시프로피온산의 생산 및 분리
JP5813512B2 (ja) リボフラビンの生産方法
CN118679240A (zh) 抗大量细胞裂解的基因修饰的细菌
CN116096881A (zh) 生产5-甲基叶酸盐的微生物
JP4824035B2 (ja) 新規遺伝子
EP2000477A1 (en) Increased production of a target product via stabilization of mRNA
EA013412B1 (ru) Новый ген sms 27

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant