CN102206584A - Garbage treatment agent and its preparation method - Google Patents
Garbage treatment agent and its preparation method Download PDFInfo
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- CN102206584A CN102206584A CN2011100734798A CN201110073479A CN102206584A CN 102206584 A CN102206584 A CN 102206584A CN 2011100734798 A CN2011100734798 A CN 2011100734798A CN 201110073479 A CN201110073479 A CN 201110073479A CN 102206584 A CN102206584 A CN 102206584A
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- waste disposal
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- disposal agent
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Abstract
The invention discloses a garbage treatment agent and its preparation method, which comprises the following components by weight: 60 to 180 parts of bacillus cereus and 100 to 200 parts of rhodopseudomonas sphaeroides. And meanwhile, the present invention also discloses a method for preparing the garbage treatment agent. According to the invention, the organic matter in the garbage can be consumed by using microbe to realize the purpose of degrading the garbage. Garbage is degraded by metabolism of microbe so that no chemical reagent is needed for adding, microbe automatically dies after finishing the metabolism and environmental secondary pollution can not occur.
Description
Technical field
The invention belongs to microorganism field, be specifically related to microorganism reagent of a kind for the treatment of refuse and preparation method thereof.
Background technology
Along with the raising of living standard, hundreds of millions of domestic refuses is arranged every day and produces rubbish to produce.What these rubbish had can not reuse, and must handle at once.In the past, the mode for the treatment of refuse was two kinds nothing more than.The one, landfill disposal, the 2nd, burn.First kind of way environmental pollution is little, but garbage degradation is slow, and the peculiar smell of generation is lasting; Stench, the obnoxious flavour of second kind of way generation are too many, and environmental pollution is serious.So, the somebody has just produced the thinking of coming treating refuse with plant extraction liquid, but doing like this is nothing but the peculiar smell that overrides rubbish with the fragrance of extracting solution, do not quicken the degraded of rubbish in essence, and often the chemical substance that contains of plant extraction liquid is poisonous, behind the life-time service, all influential to thing to the people.Therefore, administering rubbish with microbial preparation is a well design.
Summary of the invention
Goal of the invention: the objective of the invention is at the deficiencies in the prior art, a kind of microbial preparation for the treatment of refuse is provided.
Another object of the present invention is to provide the preparation method of mentioned microorganism preparation.Technical scheme: in order to reach the foregoing invention purpose, the present invention is achieved like this: a kind of waste disposal agent comprises the component of following parts by weight:
60～180 parts of bacillus cereuss
100～200 parts of Rhodopseudomonas spheroidess.
Wherein, total count 〉=2 * 10 in the described waste disposal agent
8/ ml.
Prepare the method for above-mentioned waste disposal agent, may further comprise the steps:
(1) carry out amplification culture:
Bacillus cereus through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 28～35 ℃ of conditions;
Rhodopseudomonas spheroides through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 28～35 ℃ of conditions;
Calculate viable bacteria, detect the bacterium number of bacillus cereus and Rhodopseudomonas spheroides, in proportion bacterium liquid is mixed;
(2) 25～35 ℃ of solid fermentation 36～72h, 30～35 ℃ of oven dry are pulverized, and sieve.
In order to guarantee the fineness of microbial preparation, preferentially select 40 mesh sieves in the preparation process for use.
Beneficial effect: a kind of waste disposal agent provided by the invention, utilize organism in the microbial consumption rubbish to reach the purpose of degraded rubbish.These microorganisms decomposing organic matter that can cooperatively interact is degraded into small-molecule substance with macromolecular substance, thereby reaches the purpose for the treatment of refuse.Owing to the rubbish of degrading by the metabolism of microorganism, therefore do not need to add any chemical reagent, dead voluntarily after microorganism metabolism finishes, can be to secondary environmental pollution yet.
Embodiment
Embodiment 1:
(1) carry out amplification culture respectively:
Bacillus cereus after slant activation, is inoculated in the liquid nutrient medium, cultivates 72h, liquid culture based formulas: soyflour 1.5%, starch 1%, yeast extract paste 0.2%, glucose 1%, pH6.5 for 28 ℃.
Rhodopseudomonas spheroides through slant activation, is inoculated in the liquid nutrient medium, cultivates 72h, liquid culture based formulas: ammonium chloride 0.1% for 28 ℃, sodium-acetate 3.5%, magnesium chloride 0.01%, calcium chloride 0.01%, potassium primary phosphate 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract paste 0.01%, pH6.0.
Live bacterial count detects the bacterium number of bacillus cereus and the bacterium number of Rhodopseudomonas spheroides, by weight with its mixing: 60 parts of bacillus cereuss and 100 parts of Rhodopseudomonas spheroidess.
(2) add rice bran 73%, wheat bran 10%, soyflour 7%, milk powder 8% in the solid-state fermentation tank, 1%, 121 ℃ of sterilization of trace element 30min treats to spray into mixed bacteria liquid below jar temperature drop to 40 ℃ under the situation of fermentor tank rotation, 35 ℃ of solid fermentation 36h pulverize, and cross 40 mesh sieves, and are standby.Live bacterial count, total count 〉=2 * 10
8/ ml pack.
Embodiment 2:
(1) carry out amplification culture respectively:
Bacillus cereus after slant activation, is inoculated in the liquid nutrient medium, cultivates 48h, liquid culture based formulas: soyflour 1.5%, starch 1%, yeast extract paste 0.2%, glucose 1%, pH6.5 for 32 ℃.
Rhodopseudomonas spheroides through slant activation, is inoculated in the liquid nutrient medium, cultivates 48h, liquid culture based formulas: ammonium chloride 0.1% for 32 ℃, sodium-acetate 3.5%, magnesium chloride 0.01%, calcium chloride 0.01%, potassium primary phosphate 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract paste 0.01%, pH6.0.
Live bacterial count detects the bacterium number of bacillus cereus and the bacterium number of Rhodopseudomonas spheroides, by weight with its mixing: 120 parts of bacillus cereuss and 150 parts of Rhodopseudomonas spheroidess.
(2) add rice bran 73%, wheat bran 10%, soyflour 7%, milk powder 8% in the solid-state fermentation tank, 1%, 121 ℃ of sterilization of trace element 30min treats to spray into mixed bacteria liquid below jar temperature drop to 40 ℃ under the situation of fermentor tank rotation, 28 ℃ of solid fermentation 48h pulverize, and cross 40 mesh sieves, and are standby.Live bacterial count, total count 〉=2 * 10
8/ ml pack.
Embodiment 3:
(1) carry out amplification culture respectively:
Bacillus cereus after slant activation, is inoculated in the liquid nutrient medium, cultivates 36h, liquid culture based formulas: soyflour 1.5%, starch 1%, yeast extract paste 0.2%, glucose 1%, pH6.5 for 35 ℃.
Rhodopseudomonas spheroides through slant activation, is inoculated in the liquid nutrient medium, cultivates 36h, liquid culture based formulas: ammonium chloride 0.1% for 35 ℃, sodium-acetate 3.5%, magnesium chloride 0.01%, calcium chloride 0.01%, potassium primary phosphate 0.06%, dipotassium hydrogen phosphate 0.04%, yeast extract paste 0.01%, pH6.0.
Live bacterial count detects the bacterium number of bacillus cereus and the bacterium number of Rhodopseudomonas spheroides, by weight with its mixing: 180 parts of bacillus cereuss and 200 parts of Rhodopseudomonas spheroidess.
(2) add rice bran 73%, wheat bran 10%, soyflour 7%, milk powder 8% in the solid-state fermentation tank, 1%, 121 ℃ of sterilization of trace element 30min treats to spray into mixed bacteria liquid below jar temperature drop to 40 ℃ under the situation of fermentor tank rotation, 25 ℃ of solid fermentation 72h pulverize, and cross 40 mesh sieves, and are standby.Live bacterial count, total count 〉=2 * 10
8/ ml pack.
Claims (4)
1. waste disposal agent is characterized in that it comprises the component of following parts by weight:
60～180 parts of bacillus cereuss
100～200 parts of Rhodopseudomonas spheroidess.
2. waste disposal agent according to claim 1 is characterized in that total count in the described waste disposal agent 〉=2 * 10
8/ ml.
3. the method for preparing the described waste disposal agent of claim 1 is characterized in that may further comprise the steps:
(1) carry out amplification culture:
Bacillus cereus through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 28～35 ℃ of conditions;
Rhodopseudomonas spheroides through slant activation, is inoculated in the liquid nutrient medium, cultivates 36～72h under 28～35 ℃ of conditions;
Calculate viable bacteria, detect the bacterium number of bacillus cereus and Rhodopseudomonas spheroides, in proportion bacterium liquid is mixed;
(2) 25～35 ℃ of solid fermentation 36～72h, 30～35 ℃ of oven dry are pulverized, and sieve.
4. the method for preparing waste disposal agent according to claim 3 is characterized in that step (2) selection 40 mesh sieves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100734798A CN102206584A (en) | 2011-03-25 | 2011-03-25 | Garbage treatment agent and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100734798A CN102206584A (en) | 2011-03-25 | 2011-03-25 | Garbage treatment agent and its preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102206584A true CN102206584A (en) | 2011-10-05 |
Family
ID=44695648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100734798A Pending CN102206584A (en) | 2011-03-25 | 2011-03-25 | Garbage treatment agent and its preparation method |
Country Status (1)
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| CN (1) | CN102206584A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013880A (en) * | 2012-12-20 | 2013-04-03 | 江苏碧程环保设备有限公司 | Compound microbial flocculant and preparation method and application of compound microbial flocculant |
| CN105238711A (en) * | 2015-09-23 | 2016-01-13 | 浙江富春紫光环保股份有限公司 | Preparation method for sewage treatment plant deodorant bacterial agent |
| CN108998388A (en) * | 2018-07-18 | 2018-12-14 | 梅金琪 | A kind of preparation method of waste disposal agent |
-
2011
- 2011-03-25 CN CN2011100734798A patent/CN102206584A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013880A (en) * | 2012-12-20 | 2013-04-03 | 江苏碧程环保设备有限公司 | Compound microbial flocculant and preparation method and application of compound microbial flocculant |
| CN103013880B (en) * | 2012-12-20 | 2014-04-09 | 江苏碧程环保设备有限公司 | Method for preparing compound microbial flocculant |
| CN105238711A (en) * | 2015-09-23 | 2016-01-13 | 浙江富春紫光环保股份有限公司 | Preparation method for sewage treatment plant deodorant bacterial agent |
| CN108998388A (en) * | 2018-07-18 | 2018-12-14 | 梅金琪 | A kind of preparation method of waste disposal agent |
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Application publication date: 20111005 |