CN103960480A - Method for producing micro-ecological preparation through treating kitchen wastes by utilizing enzymes and probiotics - Google Patents
Method for producing micro-ecological preparation through treating kitchen wastes by utilizing enzymes and probiotics Download PDFInfo
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- CN103960480A CN103960480A CN201410192825.8A CN201410192825A CN103960480A CN 103960480 A CN103960480 A CN 103960480A CN 201410192825 A CN201410192825 A CN 201410192825A CN 103960480 A CN103960480 A CN 103960480A
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- food waste
- lactic acid
- changing food
- acid bacteria
- lipase
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Abstract
The invention belongs to the technical field of biology and relates to a method for producing a micro-ecological preparation through cooperatively fermenting kitchen wastes by utilizing feeding lipase, yeast and lactic acid bacteria. The method specifically comprises the following steps: after the kitchen wastes are crushed and are subjected to moist heat sterilization at a high temperature, adding the feeding lipase, the yeast and a lactic acid bacteria liquid bacteria agent, fully mixing, mixing the mixture with a protective agent after aerobic fermentation and anaerobic fermentation and obtaining the micro-ecological preparation after drying. The method has the advantages that the feeding lipase can efficiently decompose oil and fat in the kitchen wastes, the yeast and the lactic acid bacteria have a good cooperative effect through co-culture, and all nutritional ingredients in the kitchen wastes can be fully utilized under the combined action of the lipase, the yeast and the lactic acid bacteria, so that the micro-ecological preparation with high live bacteria quantity is obtained.
Description
Technical field
The invention belongs to biological technical field, a kind of method that adopts lipase and saccharomycete and lactic acid bacteria associated treatment changing food waste to produce probiotics is provided, and the method all has important using value and prospect for the problem of environmental pollution that solves changing food waste with the probiotics aspect that produces Cheap highly effective.
Background technology
Changing food waste, is commonly called as hogwash, mainly comprises the remaining rubbish of meal and rubbish from cooking, is the sanitary waste that resident produces in personal consumption process.The existing serious harmfulness of changing food waste contains again a large amount of organic resources.On the one hand, changing food waste contains large quantity of moisture, very easily rots, and distributes stench, spread germs and virus, and be one of primary pollution source of urban environmental hygiene; On the other hand, changing food waste total amount is large, and content of organics is high, is rich in starch, cellulose, protein, lipid and inorganic salts, is important renewable resource.Chinese city produces every year changing food waste and is not less than 6,000 ten thousand tons at present, and presents fast rise trend with the raising of people's living standard.
At present, the processing method of changing food waste mainly contains Physical and bioanalysis, and Physical is mainly landfill and burning.Direct changing food waste landfill disposal is not only needed to take a large amount of land resource, and easily produce percolate and landfill gas and cause secondary pollution.Changing food waste water content is high, and burning disposal difficulty is large, and can produce the strong carcinogens such as bioxin.Biological treatment rule mainly relies on the effect of microorganism that changing food waste is converted into microbial manure, protein feed, ethanol, biogas or biodiesel etc., is the Main Trends of The Development of changing food waste into resources processing.But these biological methods mostly adopt more single microbial bacterial agent at present, can only utilize part nutritional labeling in changing food waste, and can bring the secondary pollutions such as waste residue and liquid.Chinese invention patent ZL201010530536.6 adopts 9 kinds of probios that changing food waste is converted into probiotics, is a kind of brand-new effectively changing food waste processing method.But the microbial bacterial agent kind that this patent is used is many, the preparation complexity of microbial inoculum.This technology adopts lipase and two kinds of probio-saccharomycete and lactic acid bacteria associated treatment changing food waste, utilize lipase to decompose fully thoroughly microorganism in changing food waste and be difficult for the oil & fat utilizing, for the growth of probio provides nutrient, and saccharomycete and lactic acid bacteria altogether cultivation and fermentation there is good cooperative effect, can make full use of so the whole nutritional labelings in changing food waste, the probiotics of synthesizing efficient cheapness.
Probiotics refers to use micro-Ecological Principle by the probio separating in animal body, and the active bacteria formulation of cultivating through special process, is called again additive for microbe feedstuff.2002 EFFCA (European food and feed bacterial classification association) provided latest definition " probio is the microorganism living, by take in can to host produce one or more special and through proving functional health benefit ".Because probiotics can promote growth of animal, improve meat, egg and milk quality and be uniquely expected to replace antibiotic, become focus and the forward position of domestic and international research and application.
Summary of the invention
At present, the processing method of changing food waste can not fully reclaim its organic resource, and can bring the secondary pollutions such as liquid and waste slag produced.Chinese invention patent ZL201010530536.6 has developed multiple probio mixed processing changing food waste and has produced the method for probiotics, though can utilize more fully the nutritional labeling of changing food waste, microbial inoculum complexity, preparation difficulty is high.In order to address these problems, the object of the invention is to adopt lipase and probio associated treatment changing food waste, make full use of whole nutritional labelings in changing food waste and produce probiotics.
Technical scheme of the present invention is, first the changing food waste after pulverizing added in rotary drum solid-state fermentation tank, and 100 DEG C of online sterilizing 30min, drop to after 28-37 DEG C until temperature, use Na
2cO
3regulate changing food waste pH to 6-7, add subsequently lipase and saccharomycete and lactic acid bacteria liquid microbial inoculum, stir.Cultivate 48-92 hour at temperature 28-37 DEG C: front 24-48 hour aerobic fermentation; Closing anaerobic fermentation in rear 24-48 hour.Ferment complete after 50 DEG C dry can obtain high viable bacteria amount probiotics.
By mass percentage, the 0.1%-0.5% that the addition of feeding lipase is changing food waste, the addition of saccharomycete and lactic acid bacteria liquid microbial inoculum is respectively the 0.2%-1% of changing food waste.
The advantage of this patent is: (1) lipase can decompose fully thoroughly microorganism and be difficult for the oil & fat in the direct changing food waste utilizing, for the growth of probio provides carbon source; (2) probio composition comprises lactic acid bacteria and two kinds of probios of saccharomycete, in cultivation and fermentation changing food waste process, there is good cooperative effect altogether: saccharomycete can produce multivitamin, ethanol, several amino acids, contribute to the growth of lactic acid bacteria, and thereby lactic acid bacteria metabolizable energy produces lactic acid impact fermentation pH, promote saccharomycetic growth.Lipase and two kinds of collaborative nutritional labelings that can make full use of in changing food waste of probio, produce efficient cheap probiotics.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is described further.
Principle of the present invention is: changing food waste mainly comprises vegetable and animals oils, the materials such as rice, vegetables, face and meat, eggs and milk, contain the compositions such as grease, cellulose, starch, carbohydrate, protein and inorganic salts from chemical composition, these all nutritional labelings that adopt lipase and saccharomycete and lactic acid bacteria can make full use of changing food waste are converted into probiotics.Lipase can provide nutrient to lactic acid bacteria and saccharomycetic growth for aliphatic acid, glycerine by grease Partial Conversion in the changing food waste that is difficult for utilizing for microorganism, two kinds of probios of lactic acid bacteria and saccharomycete can well utilize the nutritional labeling in the changing food waste except grease, and two bacterium have good cooperative effect: saccharomycete can produce multivitamin, several amino acids promotes the growth in lactic acid bacteria, and lactic acid bacteria metabolizable energy produce lactic acid utilize saccharomycetic growth.
Embodiment 1
Changing food waste after 7kg pulverizes adds in rotary drum solid-state fermentation tank, and 100 DEG C of sterilizing 30min, are cooled to 30 DEG C of temperature.Then use Na
2cO
3regulate changing food waste pH to 6, add afterwards 7g lipase, and saccharomycete and the each 14g of lactic acid bacteria liquid microbial inoculum, stir.Cultivate 96 hours 30 DEG C of temperature: first 48 hours aerobic fermentations, this stage air throughput is 1vvm, fermentation tank rotating speed is 200rpm; Closing anaerobic fermentation in latter 48 hours, fermentation tank rotating speed is 20rpm.Ferment complete after 50 DEG C can obtain total viable bacteria amount amount 48,000,000,000/g probiotics after dry.
Embodiment 2
Changing food waste after 7kg pulverizes adds in rotary drum solid-state fermentation tank, and 100 DEG C of sterilizing 30min, are cooled to 35 DEG C of temperature.Then use Na
2cO
3regulate changing food waste pH to 6, add 7g lipase and saccharomycete and the each 14g of lactic acid bacteria liquid microbial inoculum, stir.Cultivate 72 hours 35 DEG C of temperature: first 48 hours aerobic fermentations, this stage air throughput is 1vvm, fermentation tank rotating speed is 200rpm; Closing anaerobic fermentation in latter 24 hours, ferment tank rotating speed is 20rpm.Ferment complete after 50 DEG C can obtain the probiotics that total viable bacteria amount is 43,000,000,000/g after dry.
Embodiment 3
Changing food waste after 7kg pulverizes adds in rotary drum solid-state fermentation tank, and 100 DEG C of sterilizing 30min, are cooled to 37 DEG C of temperature.Then use Na
2cO
3regulate changing food waste pH to 6, add 7g lipase and saccharomycete and the each 14g of lactic acid bacteria liquid microbial inoculum, stir.Cultivate 72 hours 37 DEG C of temperature: first 24 hours aerobic fermentations, this stage air throughput is 1vvm, fermentation tank rotating speed is 200rpm; Closing anaerobic fermentation in latter 48 hours, fermentation tank rotating speed is 20rpm.Ferment and dryly after 50 DEG C after complete can obtain total viable bacteria amount amount 41,000,000,000/g probiotics.
Embodiment 4
Changing food waste after 7kg pulverizes adds in rotary drum solid-state fermentation tank, and 100 DEG C of sterilizing 30min, are cooled to 37 DEG C of temperature.Then regulate changing food waste pH to 7 with Na2CO3, add 10g lipase and saccharomycete and the each 21g of lactic acid bacteria liquid microbial inoculum, stir.Cultivate 72 hours 37 DEG C of temperature: first 24 hours aerobic fermentations, this stage air throughput is 1vvm, fermentation tank rotating speed is 200rpm; Closing anaerobic fermentation in latter 48 hours, fermentation tank rotating speed is 20rpm.Ferment and dryly after 50 DEG C after complete can obtain total viable bacteria amount amount 49,000,000,000/g probiotics.
Claims (2)
1. process the method for changing food waste production probiotics with enzyme and probio for one kind, it is characterized in that: by the 100 DEG C of moist heat sterilizations of changing food waste 30 minutes after pulverizing, drop to after 28 DEG C-37 DEG C until temperature, regulate pH to 6-7, add feeding lipase and saccharomycete and lactic acid bacteria liquid microbial inoculum, after fully mixing, at 28 DEG C-37 DEG C, after first aerobic fermentation 24-48 hour, follow anaerobic fermentation 24-48 hour, total fermentation time 48-92 hour, obtains the probiotics of high viable bacteria amount after dry at 40-60 DEG C after fermentation.
2. method according to claim 1, is characterized in that: by mass percentage, the 0.1%-0.5% that the addition of feeding lipase is changing food waste, the addition of saccharomycete and lactic acid bacteria liquid microbial inoculum is respectively the 0.2%-1% of changing food waste.
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| CN201410192825.8A CN103960480A (en) | 2014-05-08 | 2014-05-08 | Method for producing micro-ecological preparation through treating kitchen wastes by utilizing enzymes and probiotics |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085929A (en) * | 2016-08-28 | 2016-11-09 | 窦欣童 | A kind for the treatment of method for urban garbage |
| CN106623349A (en) * | 2016-10-31 | 2017-05-10 | 广州博善生物科技股份有限公司 | Kitchen waste on-site treatment method |
| CN107699522A (en) * | 2017-10-27 | 2018-02-16 | 银川保绿特生物技术有限公司 | Composite strain for biological fermentation of kitchen residual food and preparation method thereof |
| CN112616997A (en) * | 2021-01-06 | 2021-04-09 | 安徽工业大学 | Method for preparing feed by utilizing kitchen waste |
| CN113414226A (en) * | 2021-06-30 | 2021-09-21 | 云南丽天环保科技有限公司 | Kitchen waste leavening agent and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030012847A1 (en) * | 2001-05-11 | 2003-01-16 | Masahiro Yamamoto | Method for treating organic waste |
| CN101709006A (en) * | 2009-11-16 | 2010-05-19 | 天津科技大学 | Method for treating solid organic wastes by biological method |
| CN103766590A (en) * | 2012-10-28 | 2014-05-07 | 青岛嘉能节能环保技术有限公司 | Method for preparing protein fodder by using catering waste materials |
-
2014
- 2014-05-08 CN CN201410192825.8A patent/CN103960480A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030012847A1 (en) * | 2001-05-11 | 2003-01-16 | Masahiro Yamamoto | Method for treating organic waste |
| CN101709006A (en) * | 2009-11-16 | 2010-05-19 | 天津科技大学 | Method for treating solid organic wastes by biological method |
| CN103766590A (en) * | 2012-10-28 | 2014-05-07 | 青岛嘉能节能环保技术有限公司 | Method for preparing protein fodder by using catering waste materials |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085929A (en) * | 2016-08-28 | 2016-11-09 | 窦欣童 | A kind for the treatment of method for urban garbage |
| CN106085929B (en) * | 2016-08-28 | 2019-06-25 | 广东创美达环保科技有限公司 | A kind for the treatment of method for urban garbage |
| CN106623349A (en) * | 2016-10-31 | 2017-05-10 | 广州博善生物科技股份有限公司 | Kitchen waste on-site treatment method |
| CN106623349B (en) * | 2016-10-31 | 2019-05-10 | 广州博善生物科技股份有限公司 | The on-site treatment method of kitchen garbage |
| CN107699522A (en) * | 2017-10-27 | 2018-02-16 | 银川保绿特生物技术有限公司 | Composite strain for biological fermentation of kitchen residual food and preparation method thereof |
| CN112616997A (en) * | 2021-01-06 | 2021-04-09 | 安徽工业大学 | Method for preparing feed by utilizing kitchen waste |
| CN113414226A (en) * | 2021-06-30 | 2021-09-21 | 云南丽天环保科技有限公司 | Kitchen waste leavening agent and preparation method and application thereof |
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Application publication date: 20140806 |