CN102188485A - Microbial fermentation method for improving active ingredients of schisandra chinensis - Google Patents
Microbial fermentation method for improving active ingredients of schisandra chinensis Download PDFInfo
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- CN102188485A CN102188485A CN 201110081155 CN201110081155A CN102188485A CN 102188485 A CN102188485 A CN 102188485A CN 201110081155 CN201110081155 CN 201110081155 CN 201110081155 A CN201110081155 A CN 201110081155A CN 102188485 A CN102188485 A CN 102188485A
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- radix schisandrae
- schisandrae bicoloris
- microbial fermentation
- schisandra chinensis
- saccharomyces cerevisiae
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Abstract
The invention relates to a microbial fermentation method for improving active ingredients of schisandra chinensis. The method solves the problems that the conventional schisandra chinensis raw material is short and the content of the active ingredients is low. The method comprises the following steps of: 1, crushing the schisandra chinensis, and screening the crushed schisandra chinensis to obtain schisandra chinensis powder; 2, adding sterile water into the schisandra chinensis powder, sealing and sterilizing to obtain a substrate; 3, activating saccharomyces cerevisiae, and adding sterile water into the activated saccharomyces cerevisiae to form suspension; and 4, adding the suspension into the substrate, then putting the mixture into a shaking bed, culturing, taking out the cultured product, and performing freeze drying on the cultured product to obtain the fermented schisandra chinensis. The schisandra chinensis is fermented by adopting the saccharomyces cerevisiae, so that the rich enzyme system in the microbes is reacted with complex chemical components in the schisandra chinensis, and the active ingredient content of the schisandra chinensis is improved. The method is applied in the field of microbial fermentation of Chinese medicinal herbs.
Description
Technical field
The present invention relates to a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient.
Background technology
Radix Schisandrae Bicoloris to human body have QI invigorating, nourishing kidney, astringe the lung, arresting seminal emission, promote the production of body fluid, quench the thirst, Fructus Alpiniae Oxyphyllae, effect such as calm the nerves, central nervous system, respiratory system to human body have excitation, heart, liver, blood pressure there are regulating action, people's vision, audition are strengthened function; Promoting bile secretion, improve antibacterial ability, is traditional Chinese crude drug.Lignanoid is the major physiological active substance of Radix Schisandrae Bicoloris, has many-sided physiological function, and lignanoid has a variety ofly in the Radix Schisandrae Bicoloris, is mainly schisandrin, schisantherin A, deoxyschizandrin and schisandrin B etc.
Three provinces in the northeast of China are main producing regions of Radix Schisandrae Bicoloris, and Radix Schisandrae Bicoloris is mainly derived from wild resource at present, but because the minimizing of area of woods, people carry out predatoriness to it and gather in addition, and wild plant is rare, now is medicinal plants in imminent danger, cause Fructus Schisandrae Chinensis output to reduce year by year, there is lack of raw materials.And the effective ingredient in the Radix Schisandrae Bicoloris (schisandrin, schisantherin A, deoxyschizandrin and schisandrin B etc.) content is lower, is difficult to the fulfilling medicinal demand.
Summary of the invention
The present invention will solve present Radix Schisandrae Bicoloris there is lack of raw materials, and the lower problem of active constituent content, and a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient is provided.
The present invention improves the microbial fermentation processes of Radix Schisandrae Bicoloris effective ingredient, carries out according to the following steps: one, Radix Schisandrae Bicoloris is pulverized with pulverizer, crossed 40 mesh sieves then, obtain the Radix Schisandrae Bicoloris powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, saccharomyces cerevisiae is activated, add sterilized water in activatory saccharomyces cerevisiae, making concentration is 1 * 10
7The saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilization, the Radix Schisandrae Bicoloris after promptly obtaining fermenting.
The present invention adopts the fermentation by saccharomyces cerevisiae Radix Schisandrae Bicoloris, makes chemical constituent reaction complicated in enzyme system abundant in the microorganism and the Radix Schisandrae Bicoloris, has improved content of effective in the Radix Schisandrae Bicoloris.Radix Schisandrae Bicoloris after the fermentation that the present invention is obtained and the active constituent content in the Radix Schisandrae Bicoloris crude drug are made comparisons, the content of schisandrin, schisantherin A, deoxyschizandrin and schisandrin B is significantly increased, wherein the rate of increase of schisandrin can reach 31.75%, the rate of increase of schisantherin A can reach 21.34%, the rate of increase of deoxyschizandrin can reach 26.23%, and the rate of increase of schisandrin B can reach 10.52%.Therefore can use method of the present invention to improve yield in the raw material production that with acquisition schisandrin, schisantherin A, deoxyschizandrin and schisandrin B is purpose, there is lack of raw materials thereby remedy Radix Schisandrae Bicoloris, and the lower shortcoming of active constituent content.
Description of drawings
Fig. 1 is the HPLC chromatogram of four kinds of lignanoid's standard reference materials in the Fructus Schisandrae Chinensis; Fig. 2 is the HPLC chromatogram of Radix Schisandrae Bicoloris crude drug; Fig. 3 is the HPLC chromatogram of the Radix Schisandrae Bicoloris after the specific embodiment 13 fermentations.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: the microbial fermentation processes that present embodiment improves the Radix Schisandrae Bicoloris effective ingredient, carry out: one, Radix Schisandrae Bicoloris is pulverized with pulverizer, crossed 40 mesh sieves then, obtain the Radix Schisandrae Bicoloris powder according to the following steps; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, saccharomyces cerevisiae is activated, add sterilized water in activatory saccharomyces cerevisiae, making concentration is 1 * 10
7The saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilization, the Radix Schisandrae Bicoloris after promptly obtaining fermenting.
Saccharomyces cerevisiae in the present embodiment step 3 obtains for buying.
The specific embodiment two: what present embodiment and the specific embodiment one were different is: concentration of substrate is 0.3～0.6g/mL in the step 2.Other is identical with the specific embodiment one.
The specific embodiment three: what present embodiment and the specific embodiment one were different is: concentration of substrate is 0.4～0.5g/mL in the step 2.Other is identical with the specific embodiment one.
The specific embodiment four: what present embodiment and the specific embodiment one were different is: concentration of substrate is 0.37g/mL in the step 2.Other is identical with the specific embodiment one.
The specific embodiment five: what present embodiment and the specific embodiment one were different is: in the step 3 saccharomyces cerevisiae being carried out activatory concrete steps is: the saccharomyces cerevisiae of freezing preservation is put into constant incubator in 37 ℃ of activation 1 day, use inoculating loop picking saccharomyces cerevisiae to go up line then in yeast protein peptone glucose solids culture medium (YEPD culture medium), put into 37 ℃ of constant incubators then and cultivated 2 days, promptly obtain activatory saccharomyces cerevisiae.Other is identical with the specific embodiment one.
The specific embodiment six: what present embodiment was different with one of specific embodiment one to five is: in the step 4 2～7mL saccharomyces cerevisiae bacteria suspension joined in the substrate that the 75mL step 2 obtains.Other is identical with one of specific embodiment one to five.
The specific embodiment seven: what present embodiment was different with one of specific embodiment one to five is: in the step 4 3～6mL saccharomyces cerevisiae bacteria suspension joined in the substrate that the 75mL step 2 obtains.Other is identical with one of specific embodiment one to five.
The specific embodiment eight: what present embodiment was different with one of specific embodiment one to five is: in the step 4 5.32mL saccharomyces cerevisiae bacteria suspension joined in the substrate that the 75mL step 2 obtains.Other is identical with one of specific embodiment one to five.
The specific embodiment nine: what present embodiment was different with one of specific embodiment one to eight is: cultivate under 25～30 ℃ of conditions in the step 4.Other is identical with one of specific embodiment one to eight.
The specific embodiment ten: what present embodiment was different with one of specific embodiment one to eight is: cultivate under 28 ℃ of conditions in the step 4.Other is identical with one of specific embodiment one to eight.
The specific embodiment 11: what present embodiment was different with one of specific embodiment one to ten is: cultivated in the step 4 12～16 days.Other is identical with one of specific embodiment one to ten.
The specific embodiment 12: what present embodiment was different with one of specific embodiment one to ten is: cultivated 14 days in the step 4.Other is identical with one of specific embodiment one to ten.
The specific embodiment 13: the microbial fermentation processes that present embodiment improves the Radix Schisandrae Bicoloris effective ingredient, carry out: one, Radix Schisandrae Bicoloris is pulverized with pulverizer, crossed 40 mesh sieves then, obtain the Radix Schisandrae Bicoloris powder according to the following steps; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.37g/mL; Three, saccharomyces cerevisiae is activated, add sterilized water in activatory saccharomyces cerevisiae, making concentration is 1 * 10
7The saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 5.32mL saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 28 ℃ of conditions, cultivated 14 days, take out and carry out lyophilization, the Radix Schisandrae Bicoloris after promptly obtaining fermenting.
Detect the Radix Schisandrae Bicoloris after the fermentation that present embodiment makes and the active constituent content of Radix Schisandrae Bicoloris crude drug with high performance liquid chromatography (HPLC), Fig. 1 is the HPLC chromatogram of four kinds of lignanoid's standard reference materials in the Fructus Schisandrae Chinensis, Fig. 2 is the HPLC chromatogram of Radix Schisandrae Bicoloris crude drug, Fig. 3 is the HPLC chromatogram of the Radix Schisandrae Bicoloris after the present embodiment fermentation, wherein 1 is schisandrin, 2 is schisantherin A, and 3 is deoxyschizandrin, and 4 is schisandrin B.The result that records and the active constituent content of Radix Schisandrae Bicoloris crude drug sample are compared, and result data is as shown in table 1.
The Radix Schisandrae Bicoloris after table 1 fermentation and the active constituent content measuring result of Radix Schisandrae Bicoloris crude drug
As seen from the above table, the Radix Schisandrae Bicoloris active constituent content after the fermentation is compared with the Radix Schisandrae Bicoloris crude drug and is significantly increased, and proves to optimize the fermentation scheme, and the method that present embodiment is described has positive effect improving on the Radix Schisandrae Bicoloris Chinese herbal medicine effective ingredients content.
Claims (9)
1. microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient, it is characterized in that improving the microbial fermentation processes of Radix Schisandrae Bicoloris effective ingredient, carry out according to the following steps: one, Radix Schisandrae Bicoloris is pulverized with pulverizer, crossed 40 mesh sieves then, obtain the Radix Schisandrae Bicoloris powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, saccharomyces cerevisiae is activated, add sterilized water in activatory saccharomyces cerevisiae, making concentration is 1 * 10
7The saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilization, the Radix Schisandrae Bicoloris after promptly obtaining fermenting.
2. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 1 is characterized in that concentration of substrate is 0.3～0.6g/mL in the step 2.
3. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 1 is characterized in that concentration of substrate is 0.37g/mL in the step 2.
4. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 1 and 2 is characterized in that in the step 4 3～6mL saccharomyces cerevisiae bacteria suspension joined in the substrate that the 75mL step 2 obtains.
5. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 1 and 2 is characterized in that in the step 4 5.32mL saccharomyces cerevisiae bacteria suspension joined in the substrate that the 75mL step 2 obtains.
6. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 4 is characterized in that cultivating under 25～30 ℃ of conditions in the step 4.
7. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 4 is characterized in that cultivating under 28 ℃ of conditions in the step 4.
8. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 6 is characterized in that cultivating in the step 4 12～16 days.
9. a kind of microbial fermentation processes that improves the Radix Schisandrae Bicoloris effective ingredient according to claim 6 is characterized in that cultivating 14 days in the step 4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107343899A (en) * | 2017-07-11 | 2017-11-14 | 吉林医药学院 | A kind of fruit of Chinese magnoliavine snow chrysanthemum of fermenting improves formula of sleep electuary and preparation method thereof |
| CN115316658A (en) * | 2022-08-24 | 2022-11-11 | 辽宁中医药大学 | Rapid fermentation method of schisandra chinensis enzyme |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1544601A (en) * | 2003-11-25 | 2004-11-10 | 李慧梅 | Schisandra wine making process |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1544601A (en) * | 2003-11-25 | 2004-11-10 | 李慧梅 | Schisandra wine making process |
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| Title |
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| 《现代农业科技》 20070930 葛会奇 五味子果酒的酿制工艺及其质量检测 5-8 , 第9期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107343899A (en) * | 2017-07-11 | 2017-11-14 | 吉林医药学院 | A kind of fruit of Chinese magnoliavine snow chrysanthemum of fermenting improves formula of sleep electuary and preparation method thereof |
| CN107343899B (en) * | 2017-07-11 | 2021-07-30 | 吉林医药学院 | Formula of fermented schisandra chinensis and snow chrysanthemum sleep improving medicinal granules and preparation method thereof |
| CN115316658A (en) * | 2022-08-24 | 2022-11-11 | 辽宁中医药大学 | Rapid fermentation method of schisandra chinensis enzyme |
| CN115316658B (en) * | 2022-08-24 | 2023-11-28 | 辽宁中医药大学 | Fermentation method of schisandra chinensis ferment |
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