CN102091100A - Method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris - Google Patents
Method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris Download PDFInfo
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- CN102091100A CN102091100A CN2009102183631A CN200910218363A CN102091100A CN 102091100 A CN102091100 A CN 102091100A CN 2009102183631 A CN2009102183631 A CN 2009102183631A CN 200910218363 A CN200910218363 A CN 200910218363A CN 102091100 A CN102091100 A CN 102091100A
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Abstract
The invention relates to a method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris. The method mainly comprises the following steps of: (1) preparing Indian buead and cordyceps militaris strains; (2) preparing a solid fermentation culture medium of the Indian buead and the cordyceps militaris; and (3) inoculating and culturing the Indian buead and the cordyceps militaris mycelium, post-treating and detecting medicinal ingredient content. Synthetic capacity of the main medicinal ingredients of the cordyceps militaris is improved by fully utilizing abundant enzyme systems of the Indian buead and the cordyceps militaris. The method is simple and convenient in operation and good in effect, and can be popularized and applied on a large scale.
Description
Technical field
The invention belongs to the microbial fermentation technology field, relate to a kind of method that adopts Poria and Cordyceps militaris (L.) Link. to ferment altogether to improve main medicinal ingredient.
Background technology
Cordyceps militaris (L.) Link. (Cordyceps militaris L.Link) belongs to xenogenesis together with Cordyceps, is the medicine-food two-purpose fungus of generally acknowledging both at home and abroad.Cordyceps militaris (L.) Link. contains cordycepin, adenosine, polysaccharide, agglutinin isoreactivity material, has anti-tumor, antiinflammatory, antiviral, effects such as enhancing human body immunity power.Among the peoplely be used for pneumonia, suffer from a deficiency of the kidney, treatment of diseases such as lumbago, its in blood fat reducing, prevent and treat arteriosclerosis, protection heart and brain tissues, tranquilizing soporific, enhancing macrophage activity, aspects such as anticancer, antiinflammatory, antibiotic, anti-hypoxia have good efficacy.Adopting the mycelial technical method of artificial culture's Cordyceps militaris (L.) Link., can obtain to be higher than the Cordyceps culture of natural Cordyceps militaris (L.) Link. medical value at short notice, reduce the cost that people take Cordyceps militaris (L.) Link. greatly, is the effective means that effectively solves the Cordyceps imbalance between supply and demand.
Poria (Poria cocos (schw.) wolf) is the traditional rare Chinese medicine of China, according to the Compendium of Material Medica record, Poria slightly sweet flavor, property are equalled GUIXIN, lung, spleen channel, the tool spleen invigorating, calm the nerves, improve looks, calm, diuresis, also can strengthen the health immunocompetence.The main chemical constituent of Poria comprises pachyman, triterpene substance and protein etc.
For improving the content of the main medicinal ingredient of Cordyceps militaris (L.) Link. fungus filament, inoculation Poria strain in Chinese caterpillar fungus culture medium makes full use of the two abundant enzyme system, effectively transforms culture matrix.Zhou Lihong etc. have reported the method that monascus and Cordyceps militaris (L.) Link. solid ferment altogether, cordycepin content improved 2.5 times (Zhou Lihong etc., edible fungi, 2008,3:40-42).The present invention makes full use of the advantage that the Poria strain efficiently transforms carbon source and nitrogenous source in the culture medium, promotes the Cordyceps militaris (L.) Link. growth, for the accumulation of Cordyceps militaris (L.) Link. fungus filament metabolite lays the foundation, the biosynthesis ability of the main medicinal ingredient of Cordyceps militaris (L.) Link. is significantly strengthened.
Summary of the invention
The invention provides a kind of method that adopts Poria and Cordyceps militaris (L.) Link. solid to ferment altogether to improve main medicinal ingredient.
The operating procedure of this method is as follows:
(1) preparation of strain: Cordyceps militaris spawn and the Poria strain preserved on the PDA slant agar culture medium are inoculated into liquid PDA culture medium respectively, 25 ℃ of cultivation temperature, shaking speed 120r/min, activation culture 4 days, stand-by;
(2) preparation of fermentation medium altogether: by the culture medium PC-1 that preparation Poria and Cordyceps militaris (L.) Link. shown in the table 1 ferment altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Culture medium is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, activatory Cordyceps militaris spawn and Poria strain in the cooling back inoculation step (1), mixing.The Cordyceps militaris spawn inoculum concentration is 5%～15% of a culture medium gross weight, and Poria strain inoculum concentration is 5%～15% of a culture medium gross weight.The dark cultivation, 25 ℃ of cultivation temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
Table 1PC-1 medium component
Annotate: above content is mass percent.
Key point of the present invention is: the inoculum concentration of Poria strain and Cordyceps militaris spawn in the culture medium, above key point if can be got hold of, and can significantly improve Poria and the Cordyceps militaris (L.) Link. solid main pharmaceutical ingredient content of fermentation culture medium altogether.
The present invention is easy and simple to handle, and is respond well, may be used on large-scale promotion Poria and Cordyceps militaris (L.) Link. solid and is total in the actual production process of fermenting and producing.
The specific embodiment
Following examples are used to illustrate the present invention, but are not limitations of the present invention.
Embodiment 1:
(1) preparation of strain: Cordyceps militaris spawn and the Poria strain preserved on the PDA slant agar culture medium are inoculated into liquid PDA culture medium respectively, 25 ℃ of cultivation temperature, shaking speed 120r/min, activation culture 4 days, stand-by;
(2) preparation of fermentation medium altogether: by the culture medium PC-1 that preparation Poria and Cordyceps militaris (L.) Link. shown in the table 1 ferment altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Culture medium is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, cooling back activatory Cordyceps militaris spawn of inoculation and Poria strain, mixing.The Cordyceps militaris spawn inoculum concentration is 5% of a culture medium gross weight, and Poria strain inoculum concentration is 10% of a culture medium gross weight.The dark cultivation, 25 ℃ of cultivation temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria and the Cordyceps militaris (L.) Link. solid content of the main medicinal ingredient of fermentation culture medium altogether, it the results are shown in Table 2.
The main pharmaceutical ingredient content testing result of table 2 inoculation 10% Poria and 5% Cordyceps militaris spawn culture
Embodiment 2:
(1) preparation of strain: Cordyceps militaris spawn and the Poria strain preserved on the PDA slant agar culture medium are inoculated into liquid PDA culture medium respectively, 25 ℃ of cultivation temperature, shaking speed 120r/min, activation culture 4 days, stand-by;
(2) preparation of fermentation medium altogether: by the culture medium PC-1 that preparation Poria and Cordyceps militaris (L.) Link. shown in the table 1 ferment altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Culture medium is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, cooling back activatory Cordyceps militaris spawn of inoculation and Poria strain, mixing.The Cordyceps militaris spawn inoculum concentration is 10% of a culture medium gross weight, and Poria strain inoculum concentration is 5% of a culture medium gross weight.The dark cultivation, 25 ℃ of cultivation temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria and the Cordyceps militaris (L.) Link. solid content of the main medicinal ingredient of fermentation culture medium altogether, it the results are shown in Table 3.
The main pharmaceutical ingredient content testing result of table 3 inoculation 5% Poria and 10% Cordyceps militaris spawn culture
Embodiment 3:
(1) preparation of strain: Cordyceps militaris spawn and the Poria strain preserved on the PDA slant agar culture medium are inoculated into liquid PDA culture medium respectively, 25 ℃ of cultivation temperature, shaking speed 120r/min, activation culture 4 days, stand-by;
(2) preparation of fermentation medium altogether: by the culture medium PC-1 that preparation Poria and Cordyceps militaris (L.) Link. shown in the table 1 ferment altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Culture medium is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, cooling back activatory Cordyceps militaris spawn of inoculation and Poria strain, mixing.The Cordyceps militaris spawn inoculum concentration is 15% of a culture medium gross weight, and Poria strain inoculum concentration is 10% of a culture medium gross weight.The dark cultivation, 25 ℃ of cultivation temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria and the Cordyceps militaris (L.) Link. solid content of the main medicinal ingredient of fermentation culture medium altogether, it the results are shown in Table 4.
The main pharmaceutical ingredient content testing result of table 4 inoculation 15% Poria and 15% Cordyceps militaris spawn culture
Claims (1)
1. method that adopts Poria and Cordyceps militaris (L.) Link. solid to ferment altogether to improve main medicinal ingredient is characterized in that the operating procedure of this method comprises:
(1) preparation of strain: Cordyceps militaris spawn and the Poria strain preserved on the PDA slant agar culture medium are inoculated into liquid PDA culture medium respectively, 25 ℃ of cultivation temperature, shaking speed 120r/min, activation culture 4 days, stand-by;
(2) preparation of fermentation medium altogether: the culture medium PC-1 that preparation Poria and Cordyceps militaris (L.) Link. ferment altogether, material-water ratio is 1: 1.2, adjust pH is 5.8.Culture medium is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, cooling back activatory Cordyceps militaris spawn of inoculation and Poria strain, mixing.The Cordyceps militaris spawn inoculum concentration is 5%～15% of a culture medium gross weight, and Poria strain inoculum concentration is 5%～15% of a culture medium gross weight.The dark cultivation, 25 ℃ of cultivation temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293122A (en) * | 2011-06-24 | 2011-12-28 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN104686199A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis highland barley |
| CN107586811A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris bark of eucommia liquid fermentation method and the application of cordycepin content |
| CN107586726A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris rhizoma Gastrodiae liquid fermentation method and the application of cordycepin content |
| CN109689879A (en) * | 2016-09-06 | 2019-04-26 | 杏辉天力（杭州）药业有限公司 | Poria cocos tunning and its preparation method |
| CN112136972A (en) * | 2019-06-28 | 2020-12-29 | 鲁东大学 | Method for producing feed additive by combined fermentation of cordyceps militaris and traditional Chinese medicines |
| CN113730476A (en) * | 2021-09-17 | 2021-12-03 | 无锡康能特医食品科技有限公司 | Paecilomyces hepiali strain fermentation composition and preparation method and application thereof |
-
2009
- 2009-12-15 CN CN200910218363A patent/CN102091100B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293122A (en) * | 2011-06-24 | 2011-12-28 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN102293122B (en) * | 2011-06-24 | 2012-07-25 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN104686199A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis highland barley |
| CN109689879A (en) * | 2016-09-06 | 2019-04-26 | 杏辉天力（杭州）药业有限公司 | Poria cocos tunning and its preparation method |
| CN107586811A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris bark of eucommia liquid fermentation method and the application of cordycepin content |
| CN107586726A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris rhizoma Gastrodiae liquid fermentation method and the application of cordycepin content |
| CN112136972A (en) * | 2019-06-28 | 2020-12-29 | 鲁东大学 | Method for producing feed additive by combined fermentation of cordyceps militaris and traditional Chinese medicines |
| CN113730476A (en) * | 2021-09-17 | 2021-12-03 | 无锡康能特医食品科技有限公司 | Paecilomyces hepiali strain fermentation composition and preparation method and application thereof |
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| CN102091100B (en) | 2012-09-26 |
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