CN102188485B - Microbial fermentation method for improving active ingredients of schisandra chinensis - Google Patents
Microbial fermentation method for improving active ingredients of schisandra chinensis Download PDFInfo
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- CN102188485B CN102188485B CN2011100811559A CN201110081155A CN102188485B CN 102188485 B CN102188485 B CN 102188485B CN 2011100811559 A CN2011100811559 A CN 2011100811559A CN 201110081155 A CN201110081155 A CN 201110081155A CN 102188485 B CN102188485 B CN 102188485B
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- schisandra chinensis
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- saccharomyces cerevisiae
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Abstract
The invention relates to a microbial fermentation method for improving active ingredients of schisandra chinensis. The method solves the problems that the conventional schisandra chinensis raw material is short and the content of the active ingredients is low. The method comprises the following steps of: 1, crushing the schisandra chinensis, and screening the crushed schisandra chinensis to obtain schisandra chinensis powder; 2, adding sterile water into the schisandra chinensis powder, sealing and sterilizing to obtain a substrate; 3, activating saccharomyces cerevisiae, and adding sterile water into the activated saccharomyces cerevisiae to form suspension; and 4, adding the suspension into the substrate, then putting the mixture into a shaking bed, culturing, taking out the cultured product, and performing freeze drying on the cultured product to obtain the fermented schisandra chinensis. The schisandra chinensis is fermented by adopting the saccharomyces cerevisiae, so that the rich enzyme system in the microbes is reacted with complex chemical components in the schisandra chinensis, and the active ingredient content of the schisandra chinensis is improved. The method is applied in the field of microbial fermentation of Chinese medicinal herbs.
Description
Technical field
The present invention relates to a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent.
Background technology
Schisandra chinensis to human body have beneficial gas, nourshing kidney, astringe the lung, puckery essence, promote the production of body fluid, quench the thirst, intelligence development, effect such as calm the nerves; Cns, respiratory system to human body have excitation; Heart, liver, blood pressure there are regulating effect, people's eyesight, hearing are strengthened function; Promoting choleresis, improve antibacterial ability, is the traditional Chinese medicines material.Lignanoid is the major physiological active substance of Schisandra chinensis, has many-sided physiological function, and lignanoid has a variety ofly in the Schisandra chinensis, is mainly schisandrin, Wuweizi ester A, deoxyschizandrin and Wuweizisu B etc.
Three provinces in the northeast of China are main producing regions of Schisandra chinensis, and Schisandra chinensis is mainly derived from wild resource at present, but because the minimizing of area of woods; People carry out predatoriness to it and gather in addition, and wild plant is rare, is medicinal plant in imminent danger at present; Cause shizandra berry output to reduce year by year, there is lack of raw materials.And the effective constituent in the Schisandra chinensis (schisandrin, Wuweizi ester A, deoxyschizandrin and Wuweizisu B etc.) content is lower, is difficult to the fulfilling medicinal demand.
Summary of the invention
The present invention will solve present Schisandra chinensis there is lack of raw materials, and the lower problem of active constituent content, and a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent is provided.
The present invention improves the microbial fermentation processes of Schisandra chinensis effective constituent, carries out according to the following steps: one, Schisandra chinensis is pulverized with kibbler, crossed 40 mesh sieves then, obtain the Schisandra chinensis powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, yeast saccharomyces cerevisiae is carried out activation, to adding sterilized water in the activatory yeast saccharomyces cerevisiae, processing concentration is 1 * 10
7The yeast saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilize, the Schisandra chinensis after promptly obtaining fermenting.
The present invention adopts the fermentation by saccharomyces cerevisiae Schisandra chinensis, makes chemical ingredients reaction complicated in enzyme system abundant in the mikrobe and the Schisandra chinensis, has improved content of effective in the Schisandra chinensis.Schisandra chinensis after the fermentation that the present invention is obtained and the active constituent content in the Schisandra chinensis crude drug are made comparisons; The content of schisandrin, Wuweizi ester A, deoxyschizandrin and Wuweizisu B is significantly increased; Wherein the rate of increase of schisandrin can reach 31.75%; The rate of increase of Wuweizi ester A can reach 21.34%, and the rate of increase of deoxyschizandrin can reach 26.23%, and the rate of increase of Wuweizisu B can reach 10.52%.Therefore in the raw material production that with acquisition schisandrin, Wuweizi ester A, deoxyschizandrin and Wuweizisu B is purpose, can use method of the present invention to improve yield, there is lack of raw materials thereby remedy Schisandra chinensis, and the lower shortcoming of active constituent content.
Description of drawings
Fig. 1 is the HPLC color atlas of four kinds of lignanoid's standard reference materials in the shizandra berry; Fig. 2 is the HPLC color atlas of Schisandra chinensis crude drug; Fig. 3 is the HPLC color atlas of the Schisandra chinensis after embodiment 13 fermentations.
Embodiment
Technical scheme of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: this embodiment improves the microbial fermentation processes of Schisandra chinensis effective constituent, carries out according to the following steps: one, Schisandra chinensis is pulverized with kibbler, crossed 40 mesh sieves then, obtain the Schisandra chinensis powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, yeast saccharomyces cerevisiae is carried out activation, to adding sterilized water in the activatory yeast saccharomyces cerevisiae, processing concentration is 1 * 10
7The yeast saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilize, the Schisandra chinensis after promptly obtaining fermenting.
Yeast saccharomyces cerevisiae in this embodiment step 3 obtains for buying.
Embodiment two: what this embodiment and embodiment one were different is: concentration of substrate is 0.3～0.6g/mL in the step 2.Other is identical with embodiment one.
Embodiment three: what this embodiment and embodiment one were different is: concentration of substrate is 0.4～0.5g/mL in the step 2.Other is identical with embodiment one.
Embodiment four: what this embodiment and embodiment one were different is: concentration of substrate is 0.37g/mL in the step 2.Other is identical with embodiment one.
Embodiment five: what this embodiment and embodiment one were different is: in the step 3 yeast saccharomyces cerevisiae being carried out the activatory concrete steps is: the yeast saccharomyces cerevisiae of freezing preservation is put into constant incubator in 37 ℃ of activation 1 day; Use transfering loop picking yeast saccharomyces cerevisiae to go up line then at Yeast protein peptone glucose solids substratum (YEPD substratum); Put into 37 ℃ of constant incubators then and cultivated 2 days, promptly obtain the activatory yeast saccharomyces cerevisiae.Other is identical with embodiment one.
Embodiment six: what this embodiment was different with one of embodiment one to five is: in the step 4 2～7mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains.Other is identical with one of embodiment one to five.
Embodiment seven: what this embodiment was different with one of embodiment one to five is: in the step 4 3～6mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains.Other is identical with one of embodiment one to five.
Embodiment eight: what this embodiment was different with one of embodiment one to five is: in the step 4 5.32mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains.Other is identical with one of embodiment one to five.
Embodiment nine: what this embodiment was different with one of embodiment one to eight is: under 25～30 ℃ of conditions, cultivate in the step 4.Other is identical with one of embodiment one to eight.
Embodiment ten: what this embodiment was different with one of embodiment one to eight is: under 28 ℃ of conditions, cultivate in the step 4.Other is identical with one of embodiment one to eight.
Embodiment 11: what this embodiment was different with one of embodiment one to ten is: cultivated in the step 4 12～16 days.Other is identical with one of embodiment one to ten.
Embodiment 12: what this embodiment was different with one of embodiment one to ten is: cultivated 14 days in the step 4.Other is identical with one of embodiment one to ten.
Embodiment 13: this embodiment improves the microbial fermentation processes of Schisandra chinensis effective constituent, carries out according to the following steps: one, Schisandra chinensis is pulverized with kibbler, crossed 40 mesh sieves then, obtain the Schisandra chinensis powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.37g/mL; Three, yeast saccharomyces cerevisiae is carried out activation, to adding sterilized water in the activatory yeast saccharomyces cerevisiae, processing concentration is 1 * 10
7The yeast saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 5.32mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 28 ℃ of conditions, cultivated 14 days, take out and carry out lyophilize, the Schisandra chinensis after promptly obtaining fermenting.
Detect Schisandra chinensis and the active constituent content of Schisandra chinensis crude drug after the fermentation that this embodiment makes with HPLC (HPLC); Fig. 1 is the HPLC color atlas of four kinds of lignanoid's standard reference materials in the shizandra berry, and Fig. 2 is the HPLC color atlas of Schisandra chinensis crude drug, and Fig. 3 is the HPLC color atlas of the Schisandra chinensis after this embodiment fermentation; Wherein 1 is schisandrin; 2 is Wuweizi ester A, and 3 is deoxyschizandrin, and 4 is Wuweizisu B.The active constituent content of result who records and Schisandra chinensis crude drug sample is compared, and result data is as shown in table 1.
The Schisandra chinensis after table 1 fermentation and the active constituent content measuring result of Schisandra chinensis crude drug
Can find out that by last table the Schisandra chinensis active constituent content after the fermentation is compared with the Schisandra chinensis crude drug and is significantly increased, prove and optimize the fermentation scheme that the method that this embodiment is described has positive effect improving on the Schisandra chinensis Chinese herbal medicine effective ingredients content.
Claims (9)
1. microbial fermentation processes that improves Schisandra chinensis effective constituent; It is characterized in that improving the microbial fermentation processes of Schisandra chinensis effective constituent; Carry out according to the following steps: one, Schisandra chinensis is pulverized with kibbler, crossed 40 mesh sieves then, obtain the Schisandra chinensis powder; Two, add sterilized water in the Chinese Magnolivine Fruit northwards, the sealing back obtains substrate in 121 ℃ of sterilization 30min, and concentration of substrate is 0.1～0.8g/mL; Three, yeast saccharomyces cerevisiae is carried out activation, to adding sterilized water in the activatory yeast saccharomyces cerevisiae, processing concentration is 1 * 10
7The yeast saccharomyces cerevisiae bacteria suspension of cfu/mL; Four, 1～8mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains, put into shaking table then, under 128r/min, 20～36 ℃ of conditions, cultivated 11～17 days, take out and carry out lyophilize, the Schisandra chinensis after promptly obtaining fermenting.
2. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 1 is characterized in that concentration of substrate is 0.3～0.6g/mL in the step 2.
3. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 1 is characterized in that concentration of substrate is 0.37g/mL in the step 2.
4. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 1 and 2 is characterized in that in the step 4 3～6mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains.
5. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 1 and 2 is characterized in that in the step 4 5.32mL yeast saccharomyces cerevisiae bacteria suspension is joined in the substrate that the 75mL step 2 obtains.
6. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 4 is characterized in that under 25～30 ℃ of conditions, cultivating in the step 4.
7. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 4 is characterized in that under 28 ℃ of conditions, cultivating in the step 4.
8. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 6 is characterized in that cultivating in the step 4 12～16 days.
9. a kind of microbial fermentation processes that improves Schisandra chinensis effective constituent according to claim 6 is characterized in that cultivating 14 days in the step 4.
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| CN115316658B (en) * | 2022-08-24 | 2023-11-28 | 辽宁中医药大学 | Fermentation method of schisandra chinensis ferment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1544601A (en) * | 2003-11-25 | 2004-11-10 | 李慧梅 | Schisandra wine making process |
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| CN1544601A (en) * | 2003-11-25 | 2004-11-10 | 李慧梅 | Schisandra wine making process |
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| Title |
|---|
| 葛会奇.五味子果酒的酿制工艺及其质量检测.《现代农业科技》.2007,(第9期),5-8. * |
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