CN102187225B - 使用蛋白酶活化的受体鉴别调节蛋白质-蛋白质相互作用的分子 - Google Patents

使用蛋白酶活化的受体鉴别调节蛋白质-蛋白质相互作用的分子 Download PDF

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Publication number
CN102187225B
CN102187225B CN200880110461.7A CN200880110461A CN102187225B CN 102187225 B CN102187225 B CN 102187225B CN 200880110461 A CN200880110461 A CN 200880110461A CN 102187225 B CN102187225 B CN 102187225B
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protein
reporter molecule
reporter
protease
albumen
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CN102187225A (zh
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蔡济东
P·S·怀特
P·韦森瑟
H·埃辛德雷罗
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Sanofi Aventis France
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN200880110461.7A 2007-09-04 2008-08-28 使用蛋白酶活化的受体鉴别调节蛋白质-蛋白质相互作用的分子 Active CN102187225B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US96975607P 2007-09-04 2007-09-04
US60/969,756 2007-09-04
US8498708P 2008-07-30 2008-07-30
US61/084,987 2008-07-30
PCT/US2008/074543 WO2009032716A1 (en) 2007-09-04 2008-08-28 Identifying molecules that modulate protein- protein interactions using protease activated reporters

Publications (2)

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CN102187225A CN102187225A (zh) 2011-09-14
CN102187225B true CN102187225B (zh) 2015-08-05

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US (2) US8574865B2 (https=)
EP (1) EP2191275B1 (https=)
JP (1) JP5618829B2 (https=)
KR (1) KR101636306B1 (https=)
CN (1) CN102187225B (https=)
AU (1) AU2008296501B2 (https=)
BR (1) BRPI0816289B1 (https=)
CA (1) CA2698362C (https=)
CL (1) CL2008002602A1 (https=)
CY (1) CY1113816T1 (https=)
DK (1) DK2191275T3 (https=)
ES (1) ES2393642T3 (https=)
IL (1) IL204260A (https=)
MX (1) MX2010002399A (https=)
MY (1) MY155343A (https=)
PL (1) PL2191275T3 (https=)
PT (1) PT2191275E (https=)
RU (1) RU2476891C2 (https=)
TW (1) TWI425094B (https=)
UY (1) UY31323A1 (https=)
WO (1) WO2009032716A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009032716A1 (en) * 2007-09-04 2009-03-12 Sanofi-Aventis Identifying molecules that modulate protein- protein interactions using protease activated reporters
WO2014196932A1 (en) * 2013-06-06 2014-12-11 Agency For Science, Technology And Research Protease-responsive peptide biosensors and methods for analyte detection
CA2930001A1 (en) 2013-11-11 2015-05-14 University Hospitals Cleveland Medical Center Targeted treatment of anerobic cancer
CN107003323B (zh) 2014-11-25 2019-06-04 文塔纳医疗系统公司 使用化学连接和半抗原转移的邻近测定法
WO2016153880A2 (en) * 2015-03-23 2016-09-29 The Regents Of The University Of California Methods for detection of rnase activity
RU2639539C2 (ru) * 2015-12-29 2017-12-21 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) Репортерная система на основе лентивирусных репортерных конструкций для изучения белок-белковых взаимодействий
US20180301048A1 (en) * 2017-04-12 2018-10-18 Age Of Learning, Inc. Remote live tutoring platform
WO2020028381A1 (en) * 2018-07-30 2020-02-06 Montana Molecular, Llc Biosensors for detecting arrestin signaling
JP2022513606A (ja) * 2018-12-10 2022-02-09 イエール ユニバーシティ 宿主の生理機能を形成する微生物叢代謝産物
JP2022551820A (ja) * 2019-10-15 2022-12-14 エフ．ホフマン－ラ ロシュ アーゲー 細胞内または小胞内生体分子相互作用を検出するための人工膜貫通タンパク質
WO2022259904A1 (ja) * 2021-06-07 2022-12-15 国立研究開発法人理化学研究所 プロテアーゼ活性測定用の融合タンパク質基質

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1894581A (zh) * 2003-07-09 2007-01-10 森蒂金生物科学公司 检测蛋白－蛋白相互作用的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528271B1 (en) 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US5891646A (en) 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US7128915B2 (en) 1997-09-18 2006-10-31 Research Development Foundation Membrane virus host range mutations and their uses as vaccine substrates
US6884870B2 (en) 1998-03-20 2005-04-26 California Institute Of Technology Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
US20030157553A1 (en) 2000-03-03 2003-08-21 Gabriel Berstein Methods of assaying for G protein-coupled receptor ligands and modulators
EP1205191A1 (en) 2000-11-13 2002-05-15 Centre National De La Recherche Scientifique Targeted modification of intracellular compounds
EP1353182A3 (en) 2002-04-12 2004-02-04 Smithkline Beecham Corporation Method of predicting cell-based assay results using binding profiles
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
US7524925B2 (en) 2004-11-18 2009-04-28 Washington State University Protein interaction reporter agents and methods for using same
WO2009032716A1 (en) * 2007-09-04 2009-03-12 Sanofi-Aventis Identifying molecules that modulate protein- protein interactions using protease activated reporters
EP3508570B1 (en) * 2010-05-11 2020-07-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1894581A (zh) * 2003-07-09 2007-01-10 森蒂金生物科学公司 检测蛋白－蛋白相互作用的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Monitoring regulated protein-protein interactions using split TEV;Wehr Michael C et al;《Nature Methods》;20061201;第3卷(第12期);985-993 *

Also Published As

Publication number Publication date
WO2009032716A1 (en) 2009-03-12
US20100311096A1 (en) 2010-12-09
PL2191275T3 (pl) 2013-01-31
JP5618829B2 (ja) 2014-11-05
CY1113816T1 (el) 2016-07-27
DK2191275T3 (da) 2012-11-26
MY155343A (en) 2015-10-15
CL2008002602A1 (es) 2009-06-05
AU2008296501B2 (en) 2014-07-03
JP2010537669A (ja) 2010-12-09
BRPI0816289A2 (pt) 2016-11-01
CN102187225A (zh) 2011-09-14
US8574865B2 (en) 2013-11-05
IL204260A (en) 2013-12-31
KR20100058526A (ko) 2010-06-03
TW200925281A (en) 2009-06-16
KR101636306B1 (ko) 2016-07-05
CA2698362C (en) 2013-03-26
AU2008296501A1 (en) 2009-03-12
HK1162664A1 (zh) 2012-08-31
UY31323A1 (es) 2009-04-30
MX2010002399A (es) 2010-03-25
PT2191275E (pt) 2012-11-16
TWI425094B (zh) 2014-02-01
US20140162289A1 (en) 2014-06-12
RU2010112854A (ru) 2011-10-10
RU2476891C2 (ru) 2013-02-27
EP2191275B1 (en) 2012-08-08
ES2393642T3 (es) 2012-12-26
EP2191275A1 (en) 2010-06-02
BRPI0816289B1 (pt) 2019-01-15
BRPI0816289A8 (pt) 2018-05-08
WO2009032716A8 (en) 2011-02-17
CA2698362A1 (en) 2009-03-12
US9023610B2 (en) 2015-05-05

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