CN102177250B - 由粗制核酸样品直接扩增的方法 - Google Patents

由粗制核酸样品直接扩增的方法 Download PDF

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Publication number
CN102177250B
CN102177250B CN200980134038.5A CN200980134038A CN102177250B CN 102177250 B CN102177250 B CN 102177250B CN 200980134038 A CN200980134038 A CN 200980134038A CN 102177250 B CN102177250 B CN 102177250B
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China
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dna
nucleic acid
direct
pcr
bsa
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Expired - Fee Related
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Chinese (zh)
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CN102177250A (zh
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C·张
D·Y·王
L·K·亨尼西
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Life Technologies Corp
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Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN200980134038.5A 2008-06-30 2009-06-30 由粗制核酸样品直接扩增的方法 Expired - Fee Related CN102177250B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7711308P 2008-06-30 2008-06-30
US61/077113 2008-06-30
PCT/US2009/049323 WO2010002938A2 (en) 2008-06-30 2009-06-30 Method for direct amplification from crude nucleic acid samples

Publications (2)

Publication Number Publication Date
CN102177250A CN102177250A (zh) 2011-09-07
CN102177250B true CN102177250B (zh) 2015-05-06

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CN200980134038.5A Expired - Fee Related CN102177250B (zh) 2008-06-30 2009-06-30 由粗制核酸样品直接扩增的方法

Country Status (10)

Country Link
US (4) US8173401B2 (https=)
EP (2) EP2294225B8 (https=)
JP (2) JP5770085B2 (https=)
CN (1) CN102177250B (https=)
BR (1) BRPI0915886A2 (https=)
DK (1) DK2294225T3 (https=)
ES (1) ES2532632T3 (https=)
HR (1) HRP20150280T1 (https=)
SI (1) SI2294225T1 (https=)
WO (1) WO2010002938A2 (https=)

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US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
WO2012170907A2 (en) * 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
EP2574669B1 (en) * 2011-06-29 2018-10-17 Kabushiki Kaisha Dnaform Biological sample pretreatment method, method for detecting rna, and pretreatment kit
BR112014000138B1 (pt) * 2011-07-06 2021-11-16 Quest Diagnostics Investments Incorporation Método para identificar a presença ou ausência de um ácido nucleico alvo
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
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US9354159B2 (en) 2012-05-02 2016-05-31 Nanoscopia (Cayman), Inc. Opto-fluidic system with coated fluid channels
GB201219137D0 (en) * 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
CN104769111B (zh) * 2012-11-09 2021-04-27 环球生命科学解决方案运营英国有限公司 用于非洗提样品的一步法核酸扩增的方法
EP2971109B1 (en) * 2013-03-15 2018-10-24 GE Healthcare UK Limited Methods for one step nucleic acid amplification of non-eluted samples
US10017807B2 (en) * 2013-03-15 2018-07-10 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015096063A1 (en) 2013-12-25 2015-07-02 Coyote Bioscience Co., Ltd. Methods and systems for nucleic acid amplification
CN103789299B (zh) * 2014-01-09 2016-08-31 东北制药集团辽宁生物医药有限公司 一种进行全血基因组快速扩增的方法
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
EP3221704A4 (en) * 2014-11-20 2018-04-11 GE Healthcare UK Limited Detecting dementia and alzheimer's disease associated biomarkers stabilized on solid support materials
WO2017180745A1 (en) * 2016-04-14 2017-10-19 T2 Biosystems, Inc. Methods and systems for amplification in complex samples
CN105925692B (zh) * 2016-05-16 2019-09-20 安陆市疾病预防控制中心 一种常温稳定的pcr预混液
WO2018017884A1 (en) 2016-07-20 2018-01-25 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2019060628A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
CN107937491A (zh) * 2017-11-30 2018-04-20 广州定康医疗科技有限公司 一种针对口腔拭子的基于pcr的snp基因分型方法

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Publication number Publication date
US20140120541A1 (en) 2014-05-01
WO2010002938A2 (en) 2010-01-07
EP2294225A2 (en) 2011-03-16
US8173401B2 (en) 2012-05-08
EP2294225B8 (en) 2015-02-18
CN102177250A (zh) 2011-09-07
HRP20150280T1 (hr) 2015-04-10
US20100015621A1 (en) 2010-01-21
WO2010002938A3 (en) 2010-04-08
EP2294225A4 (en) 2011-10-26
US20130005609A1 (en) 2013-01-03
US9057110B2 (en) 2015-06-16
ES2532632T3 (es) 2015-03-30
BRPI0915886A2 (pt) 2015-11-03
JP2014166189A (ja) 2014-09-11
US20150354015A1 (en) 2015-12-10
EP2294225B1 (en) 2014-12-17
DK2294225T3 (en) 2015-03-30
EP2905344A1 (en) 2015-08-12
JP5770085B2 (ja) 2015-08-26
SI2294225T1 (sl) 2015-04-30
US8460934B2 (en) 2013-06-11
US9518302B2 (en) 2016-12-13
JP2011526788A (ja) 2011-10-20
EP2905344B1 (en) 2016-11-09

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