ES2532632T3 - Método de amplificación directa a partir de muestras de ácido nucleico en bruto - Google Patents
Método de amplificación directa a partir de muestras de ácido nucleico en bruto Download PDFInfo
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- ES2532632T3 ES2532632T3 ES09774388.4T ES09774388T ES2532632T3 ES 2532632 T3 ES2532632 T3 ES 2532632T3 ES 09774388 T ES09774388 T ES 09774388T ES 2532632 T3 ES2532632 T3 ES 2532632T3
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- direct buffer
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7711308P | 2008-06-30 | 2008-06-30 | |
| US77113P | 2008-06-30 | ||
| PCT/US2009/049323 WO2010002938A2 (en) | 2008-06-30 | 2009-06-30 | Method for direct amplification from crude nucleic acid samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2532632T3 true ES2532632T3 (es) | 2015-03-30 |
Family
ID=41466583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09774388.4T Active ES2532632T3 (es) | 2008-06-30 | 2009-06-30 | Método de amplificación directa a partir de muestras de ácido nucleico en bruto |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US8173401B2 (https=) |
| EP (2) | EP2294225B8 (https=) |
| JP (2) | JP5770085B2 (https=) |
| CN (1) | CN102177250B (https=) |
| BR (1) | BRPI0915886A2 (https=) |
| DK (1) | DK2294225T3 (https=) |
| ES (1) | ES2532632T3 (https=) |
| HR (1) | HRP20150280T1 (https=) |
| SI (1) | SI2294225T1 (https=) |
| WO (1) | WO2010002938A2 (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2294225B8 (en) | 2008-06-30 | 2015-02-18 | Life Technologies Corporation | Method for direct amplification from crude nucleic acid samples |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| US8911941B2 (en) * | 2011-04-14 | 2014-12-16 | Kenneth J. Michlitsch | Methods and apparatus for point-of-care nucleic acid amplification and detection |
| US9469871B2 (en) | 2011-04-14 | 2016-10-18 | Corporos Inc. | Methods and apparatus for point-of-care nucleic acid amplification and detection |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| WO2012170907A2 (en) * | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| FI3461807T3 (fi) | 2011-06-08 | 2023-09-07 | Life Technologies Corp | Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten |
| EP2574669B1 (en) * | 2011-06-29 | 2018-10-17 | Kabushiki Kaisha Dnaform | Biological sample pretreatment method, method for detecting rna, and pretreatment kit |
| BR112014000138B1 (pt) * | 2011-07-06 | 2021-11-16 | Quest Diagnostics Investments Incorporation | Método para identificar a presença ou ausência de um ácido nucleico alvo |
| US10093975B2 (en) | 2011-12-01 | 2018-10-09 | Genapsys, Inc. | Systems and methods for high efficiency electronic sequencing and detection |
| GB201201464D0 (en) | 2012-01-27 | 2012-03-14 | Secr Defence | Analytical method |
| US9354159B2 (en) | 2012-05-02 | 2016-05-31 | Nanoscopia (Cayman), Inc. | Opto-fluidic system with coated fluid channels |
| GB201219137D0 (en) * | 2012-10-24 | 2012-12-05 | Ge Healthcare Uk Ltd | Direct nucleic acid amplification kit, reagent and method |
| CN104769111B (zh) * | 2012-11-09 | 2021-04-27 | 环球生命科学解决方案运营英国有限公司 | 用于非洗提样品的一步法核酸扩增的方法 |
| EP2971109B1 (en) * | 2013-03-15 | 2018-10-24 | GE Healthcare UK Limited | Methods for one step nucleic acid amplification of non-eluted samples |
| US10017807B2 (en) * | 2013-03-15 | 2018-07-10 | Verinata Health, Inc. | Generating cell-free DNA libraries directly from blood |
| EP2971141B1 (en) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systems for biological analysis |
| EP3539944A1 (en) | 2013-10-25 | 2019-09-18 | Life Technologies Corporation | Novel compounds for use in pcr systems and applications thereof |
| WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
| WO2015096063A1 (en) | 2013-12-25 | 2015-07-02 | Coyote Bioscience Co., Ltd. | Methods and systems for nucleic acid amplification |
| CN103789299B (zh) * | 2014-01-09 | 2016-08-31 | 东北制药集团辽宁生物医药有限公司 | 一种进行全血基因组快速扩增的方法 |
| US9822401B2 (en) | 2014-04-18 | 2017-11-21 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| EP3221704A4 (en) * | 2014-11-20 | 2018-04-11 | GE Healthcare UK Limited | Detecting dementia and alzheimer's disease associated biomarkers stabilized on solid support materials |
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| US20150354015A1 (en) | 2015-12-10 |
| EP2294225B1 (en) | 2014-12-17 |
| DK2294225T3 (en) | 2015-03-30 |
| EP2905344A1 (en) | 2015-08-12 |
| JP5770085B2 (ja) | 2015-08-26 |
| SI2294225T1 (sl) | 2015-04-30 |
| US8460934B2 (en) | 2013-06-11 |
| US9518302B2 (en) | 2016-12-13 |
| JP2011526788A (ja) | 2011-10-20 |
| EP2905344B1 (en) | 2016-11-09 |
| CN102177250B (zh) | 2015-05-06 |
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