CN102146361A - 一种分离纯化谷氨酰胺转胺酶的方法 - Google Patents
一种分离纯化谷氨酰胺转胺酶的方法 Download PDFInfo
- Publication number
- CN102146361A CN102146361A CN 201110034437 CN201110034437A CN102146361A CN 102146361 A CN102146361 A CN 102146361A CN 201110034437 CN201110034437 CN 201110034437 CN 201110034437 A CN201110034437 A CN 201110034437A CN 102146361 A CN102146361 A CN 102146361A
- Authority
- CN
- China
- Prior art keywords
- volume
- transglutaminase
- resin
- amount
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108060008539 Transglutaminase Proteins 0.000 title claims abstract description 52
- 102000003601 transglutaminase Human genes 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 55
- 108090000790 Enzymes Proteins 0.000 claims abstract description 55
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 18
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000011347 resin Substances 0.000 claims description 33
- 229920005989 resin Polymers 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 16
- 238000013016 damping Methods 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 15
- 239000002253 acid Substances 0.000 claims description 13
- 238000000926 separation method Methods 0.000 claims description 11
- 238000000746 purification Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 8
- 125000002091 cationic group Chemical group 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 8
- 230000014759 maintenance of location Effects 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 8
- 238000001556 precipitation Methods 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 238000004062 sedimentation Methods 0.000 claims description 8
- 238000000108 ultra-filtration Methods 0.000 claims description 8
- 230000002378 acidificating effect Effects 0.000 claims description 6
- GHXRKGHKMRZBJH-UHFFFAOYSA-N boric acid Chemical compound OB(O)O.OB(O)O GHXRKGHKMRZBJH-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 150000002500 ions Chemical class 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 229940023913 cation exchange resins Drugs 0.000 claims description 5
- 229920001429 chelating resin Polymers 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 229920001467 poly(styrenesulfonates) Polymers 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 2
- 150000003016 phosphoric acids Chemical class 0.000 claims description 2
- 239000007974 sodium acetate buffer Substances 0.000 claims description 2
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims description 2
- 239000004328 sodium tetraborate Substances 0.000 claims description 2
- WUWHFEHKUQVYLF-UHFFFAOYSA-M sodium;2-aminoacetate Chemical compound [Na+].NCC([O-])=O WUWHFEHKUQVYLF-UHFFFAOYSA-M 0.000 claims description 2
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 238000011084 recovery Methods 0.000 description 6
- 239000012149 elution buffer Substances 0.000 description 4
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- VJHCJDRQFCCTHL-UHFFFAOYSA-N acetic acid 2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)=O.OCC(O)C(O)C(O)C(O)C=O VJHCJDRQFCCTHL-UHFFFAOYSA-N 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000001641 gel filtration chromatography Methods 0.000 description 2
- LCGISIDBXHGCDW-VKHMYHEASA-N L-glutamine amide Chemical group NC(=O)[C@@H](N)CCC(N)=O LCGISIDBXHGCDW-VKHMYHEASA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 239000012505 Superdex™ Substances 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000002478 diastatic effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- -1 phenolic aldehyde Chemical class 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 201110034437 CN102146361B (zh) | 2011-02-01 | 2011-02-01 | 一种分离纯化谷氨酰胺转胺酶的方法 |
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CN 201110034437 CN102146361B (zh) | 2011-02-01 | 2011-02-01 | 一种分离纯化谷氨酰胺转胺酶的方法 |
Publications (2)
Publication Number | Publication Date |
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CN102146361A true CN102146361A (zh) | 2011-08-10 |
CN102146361B CN102146361B (zh) | 2013-06-19 |
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CN 201110034437 Active CN102146361B (zh) | 2011-02-01 | 2011-02-01 | 一种分离纯化谷氨酰胺转胺酶的方法 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102719411A (zh) * | 2012-06-18 | 2012-10-10 | 华东师范大学 | 一种微生物谷氨酰胺转胺酶及其应用 |
CN106011099A (zh) * | 2016-06-23 | 2016-10-12 | 华东理工大学 | 分离纯化昆虫谷氨酰胺转氨酶的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5731183A (en) * | 1995-02-09 | 1998-03-24 | Ajinomoto Co., Inc. | Bacillus-derived transglutaminase |
CN1334867A (zh) * | 1998-12-28 | 2002-02-06 | 味之素株式会社 | 转谷氨酰胺酶的制备方法 |
-
2011
- 2011-02-01 CN CN 201110034437 patent/CN102146361B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5731183A (en) * | 1995-02-09 | 1998-03-24 | Ajinomoto Co., Inc. | Bacillus-derived transglutaminase |
CN1334867A (zh) * | 1998-12-28 | 2002-02-06 | 味之素株式会社 | 转谷氨酰胺酶的制备方法 |
Non-Patent Citations (2)
Title |
---|
《[J]. Agric. Biol. Chem》 19891231 Ando H,Adachi M,Umeda K,et al. Purification and characteristics of a novel transglutaminase derived from microorganisms 2613-2617 1-7 第53卷, * |
《食品与发酵工业》 20051231 尹少谦 王 淼 St reptomyces hygroscopicus 谷氨酰胺转胺酶的分离纯化及其性质研究 26-29 1-7 第31卷, 第3期 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102719411A (zh) * | 2012-06-18 | 2012-10-10 | 华东师范大学 | 一种微生物谷氨酰胺转胺酶及其应用 |
CN102719411B (zh) * | 2012-06-18 | 2014-06-25 | 华东师范大学 | 一种微生物谷氨酰胺转胺酶及其应用 |
CN106011099A (zh) * | 2016-06-23 | 2016-10-12 | 华东理工大学 | 分离纯化昆虫谷氨酰胺转氨酶的方法 |
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CN102146361B (zh) | 2013-06-19 |
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Address after: The root of 225433 Taixing Road, Jiangsu Yiming home city in Taizhou Province Patentee after: JIANGSU YIMING BIOLOGICAL CO.,LTD. Address before: The root of 225433 Taixing Road, Jiangsu Yiming home city in Taizhou Province Patentee before: YIMING BIOLOGICAL PRODUCTS Co.,Ltd. |
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Denomination of invention: A Method for Separation and Purification of Transglutaminase Effective date of registration: 20230721 Granted publication date: 20130619 Pledgee: Jiangsu Changjiang Commercial Bank Co.,Ltd. Taixing Branch Pledgor: JIANGSU YIMING BIOLOGICAL CO.,LTD. Registration number: Y2023980049356 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20130619 Pledgee: Jiangsu Changjiang Commercial Bank Co.,Ltd. Taixing Branch Pledgor: JIANGSU YIMING BIOLOGICAL CO.,LTD. Registration number: Y2023980049356 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for separating and purifying glutamine transaminase Granted publication date: 20130619 Pledgee: Jiangsu Changjiang Commercial Bank Co.,Ltd. Taixing Branch Pledgor: JIANGSU YIMING BIOLOGICAL CO.,LTD. Registration number: Y2024980022801 |