CN102094044B - Method for preparing corn stigma extract, extract and using method of extract - Google Patents
Method for preparing corn stigma extract, extract and using method of extract Download PDFInfo
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Abstract
The invention provides a method for preparing corn stigma extract, extract and a using method of extract. The method for preparing the corn stigma extract comprises the following steps of: preparing paste, namely crushing corn stigma, adding water and mixing to prepare paste; fermenting, namely adding fermentation strains into paste and fermenting to obtain fermented products; lixiviating, namely, adding an organic solvent into the fermented products and lixiviating; and filtering, namely after filtering, and filtering the organic solvent to obtain the corresponding corn stigma extract.
Description
Technical field
The present invention relates to a kind of preparation method, extract and method of use thereof of Stigma Maydis extract, relate in particular to preparation method, extract and the method for use thereof of a kind of Stigma Maydis extract in the tobacco manufacturing field.
Background technology
Stigma Maydis (Corn Stigma) has another name called Zea mays stamen, ear of maize hair, is the style of grass Semen Maydis.
The medicinal efficacy of Stigma Maydis is included in " the southern regions of the Yunnan Province book on Chinese herbal medicine " the earliest, and its property is flat, and it is sweet, nontoxic to distinguish the flavor of, and has clearing away heat and promoting diuresis; Expel the heat-evil, flat liver, cholagogic, detumescence, hypotensive effect is mainly used in oedema due to nephritis; dysuria, mellitus, gallbladdergallstonecholetithiasis, hepatitis jaundice, essential hypertension; beriberi, cholecystitis, hematemesis and epistaxis, nasosinusitis, acute mastitis etc." Sichuan Chinese medicinal herbal " record must be dried with Zea mays, inhales it on the dress tobacco tube, can control brain and leak (nasosinusitis).
Contain saponin in the Stigma Maydis, wax, volatile oil, vegeto-alkali, bitter glucoside, resin, mineral substance, polysaccharide, saltpetre, kryptoxanthin, vitamins C, pantothenic acid, oxysuccinic acid, Citric Acid, tartrate, oxalic acid, Sitosterol, amino acid, vitamin K3, vitamin E etc.Wherein volatile oil is pure with 5-alkene-3-, β-Gu Zaichun, and beans alcohol-7-alkene-3-alcohol is staple.Amino acid has 16 kinds, contains 7 kinds of essential amino acids, like Threonine, L-glutamic acid, Serine etc.Carbohydrate mainly contains glucose, piperylene, Polygalactan.
Corn all has cultivation in China various places, and the Stigma Maydis resource is very abundant, but at present very low to its development and use degree, only has on a small quantity and is used as medicine.Disclosed Chinese patent about Stigma Maydis (application) has 2: 02117930.1 (corn tassel injection and preparation method thereof) and 200410011071.8 (Chinese medicine Stigma Maydis preparation and preparation method thereof).
The document that relates to Stigma Maydis mainly is the chemical ingredients about Stigma Maydis, pharmacological action, the extraction of polysaccharide research in anti-oxidant activity and the Stigma Maydis.Studied the anti-oxidant activity of Stigma Maydis extract like Liu's equality [Jiangsu agricultural journal, 2006,22 (3): P293 to 296], the result shows that in the DPPH system, the n-butyl alcohol extract anti-oxidant activity of Stigma Maydis is the strongest, and its scavenging(action) and rutin are almost suitable.Answer the rare talent to wait [Chinese biochemical drug magazine, 2006,27 (4): 233-234] to obtain extracting the optimum process condition of corn silk polysaccharide through optimization of orthogonal test.
Summary of the invention
In view of this, technical problem to be solved by this invention provides a kind of preparation method, extract and method of use thereof of Stigma Maydis extract, so that efficient Stigma Maydis is handled; To obtain more excellent extract; And can be applied to tobacco manufacturing field, the cigarette interior quality that finally is improved increases cigarette flavor; Dried-up assorted gas alleviates, the effect of the sweet property raising of flue gas.
The objective of the invention is to realize through following technical scheme:
A kind of preparation method of Stigma Maydis extract is characterized in that, comprises the steps:
A kind of preparation method of Stigma Maydis extract is characterized in that, comprises the steps:
Become to stick with paste step: Stigma Maydis is pulverized, added water furnishing mashed prod;
Fermentation step: add fermented bacterium at this mashed prod, ferment, obtain tunning;
Lixiviate step: in this tunning, add organic solvent, carry out lixiviate, to obtain corresponding Stigma Maydis extract.
Adopt technique scheme; The corresponding Stigma Maydis extract of acquisition that can efficient, generally speaking, this Stigma Maydis extract is faint yellow to pale brown look fluxion; Has the cereal characteristic perfume; Also have simultaneously have baking fragrant with fermentation fragrance, can water-soluble, Ucar 35, or volume percent is lower than 70% ethanolic soln.
Preferably, said fermented bacterium adopts yeast saccharomyces cerevisiae, and the inoculum size of said yeast saccharomyces cerevisiae is every gram Stigma Maydis 1 * 10
3To 1 * 10
6Cfu.
Through adopting yeast saccharomyces cerevisiae that Stigma Maydis is fermented, not only with low cost, be convenient to also make that the part macromolecular substance in the Stigma Maydis is degraded, especially be convenient to produce more flavour ingredient.
Preferably, in the said fermentation step, temperature is 28 to 40 ℃, continues 24 to 72 hours.
Preferably, said one-tenth is stuck with paste in the step, pulverizes after the said Stigma Maydis drying, and the particle diameter of its pulverized particles is 250 to 830 μ m, and the amount that adds entry then is 2 to 5 times of said Stigma Maydis weight.
Through overtesting this is proved, further adopt abovementioned technology, the extraction efficiency of raising Stigma Maydis extract that will be bigger can obtain more Stigma Maydis extract under the equal conditions.
Preferably, in the said lixiviate step, said organic solvent selects to adopt ethanol, acetone or Ucar 35, perhaps above-mentioned three's the aqueous solution; The weight of the said organic solution that is added for 4 to 10 times of said Stigma Maydis weight, is under 55 to 110 ℃ in temperature, and extraction time continues 2 to 8 hours.
Wherein, select different organic solvents for use, will be convenient to extract opposed polarity and deliquescent material in the Stigma Maydis.
Preferably, the preparation method of described Stigma Maydis extract also comprises:
Filtration step: after the lixiviate step, this Stigma Maydis extract is filtered, to obtain corresponding filtrating.
Preferably, in the said filtration step, adopt the method for screen filtration, this mesh size is 38 to 58 μ m.
Filtration step also makes Stigma Maydis extract have the more stability of good state and Geng Gao.
In order further to improve the state and the stability of Stigma Maydis extract, the preparation method of described Stigma Maydis extract also comprises:
Enrichment step: concentrate for said filtrating, further obtain 0.2 to 2 times, the spissated Stigma Maydis extract of said Stigma Maydis weight.
Preferably, in the said enrichment step, concentrated pressure is 0.8 * 10
4To 1 * 10
5Pa, thickening temperature are 40 ℃～100 ℃.
The present invention also provides, a kind of Stigma Maydis extract that adopts described preparation method to obtain.
Generally speaking, this Stigma Maydis extract is faint yellow to pale brown look fluxion, has the cereal characteristic perfume, also have simultaneously have baking fragrant with fermentation fragrance, can water-soluble, Ucar 35, or volume percent is lower than 70% ethanolic soln.Certainly, because concrete technique means that said preparation method taked is variant, the Stigma Maydis extract that it obtained is in also corresponding some differences that have in nature.
The present invention also provides a kind of method of use of described Stigma Maydis extract, comprises the steps:
Dilution step: said Stigma Maydis extract is added organic diluting solvent get into dilution, obtain diluent;
Spray step: said diluent is sprayed the tobacco leaf surface in flue-cured tobacco.
Adopt technique scheme, through overtesting contrast proof, with the interior quality of greatly having improved cigarette, cigarette fragrance is increased, dried-up assorted gas alleviates, the sweet property raising of flue gas.
In general, organic diluting solvent is chosen as nontoxic and tasteless organic solvent.
Preferably, said organic diluting solvent selects to adopt Ucar 35, sorbyl alcohol, Xylitol or glycerine, perhaps above four the aqueous solution.
Preferably, extension rate is 5 to 20 times in the said dilution step.
Because the Stigma Maydis extract that adopted has difference, for example spissated Stigma Maydis extract or not between the Stigma Maydis extract concentration bigger difference is just arranged, can select extension rate according to practical situation.
Preferably, said spraying in the step, the amount of spraying of said diluent accounts for its flue-cured tobacco weight that sprays 0.1% to 1.0%.
Embodiment
Below preferred embodiment of the present invention the present invention is described further.
A kind of embodiment of the preparation method of Stigma Maydis extract of the present invention utilizes Stigma Maydis, through drying and crushing, adds water and becomes pasty state, and inoculation fermentation carries out lixiviate with moisture or water-free organic solvent again, obtains Stigma Maydis extract through filtering and concentrating.Drying and crushing helps improving the extraction yield of Stigma Maydis; And, can make the macromolecular substance degraded in the Stigma Maydis through yeast fermentation, produce more flavour ingredient; Adopt different organic solvent extractions, can make opposed polarity and deliquescent material be able to extraction; Filtration and concentration process have guaranteed that then the Stigma Maydis extract of finished product has the stability of good state and Geng Gao.
Stigma Maydis extract is applied to cigarette, has greatly improved the interior quality of cigarette, cigarette fragrance is increased, dried-up assorted gas alleviates, the sweet property raising of flue gas.
Embodiment in conjunction with the preparation method of further concrete Stigma Maydis extract, below do further detailed description:
Embodiment 1:
Get Stigma Maydis 100g and be crushed to particle diameter 830 μ m, put into beaker, add 500g water, sterilized 30 minutes for 85 ℃.Add the yeast saccharomyces cerevisiae bacterial classification, inoculum size is 1 * 10
6Cfu/g after stirring, leaves standstill under 40 ℃ and cultivated 24 hours.The aqueous ethanolic solution that adds 400g80% (v/v), 88 ℃ of refluxed lixiviates 4 hours are cooled to room temperature.Screen filtration with 48 μ m apertures must be filtrated.Filtrating is at concentrated pressure 1.0 * 10
4Under the Pa, 50 ℃ of thickening temperatures, be evaporated to 50g, Stigma Maydis extract I.
Embodiment 2:
Get Stigma Maydis 100g and be crushed to particle diameter 250 μ m, put into beaker, add 300g water, sterilized 30 minutes for 85 ℃.Add the yeast saccharomyces cerevisiae bacterial classification, inoculum size is 1 * 10
4Cfu/g after stirring, leaves standstill under 30 ℃ and cultivated 48 hours.Add 800g acetone, 56 ℃ of refluxed lixiviates 8 hours are cooled to room temperature, must filtrate with the screen filtration in 38 μ m apertures.Be concentrated into 20g under the filtrating normal pressure, get Stigma Maydis extract II.
Embodiment 3:
Get Stigma Maydis 100g and be crushed to particle diameter 380 μ m, put into beaker, add 400g water, sterilized 30 minutes for 85 ℃.Add the yeast saccharomyces cerevisiae bacterial classification, inoculum size is 1 * 10
3Cfu/g after stirring, leaves standstill under 28 ℃ and cultivated 72 hours.20% (mass ratio) aqueous solution of propylene glycol that adds 500g, 100 ℃ of refluxed lixiviates 2 hours are cooled to room temperature, must filtrate with the screen filtration in 48 μ m apertures.Filtrating is at concentrated pressure 0.8 * 10
4Under the Pa, 50 ℃ of thickening temperatures, be evaporated to 200g, Stigma Maydis extract III.
Comparison test:
Stigma Maydis extract I in the foregoing description 1,2,3, II, III are diluted to 10% with 50% aqueous solution of propylene glycol earlier respectively, are sprayed at a kind of tobacco leaf surface of flue-cured tobacco then.Applied amount is respectively 0.1%, 0.2%, 0.5%, 1.0% of flue-cured tobacco weight; Be control samples with the flue-cured tobacco that does not spray Stigma Maydis extract simultaneously; Subsequent disposal can be undertaken by the cigarette process of routine; Promptly after spraying the diluent of Stigma Maydis extract, stored 4 hours, process cigarette finished product through chopping, baking silk, perfuming, storage silk, cigarette and packaging process again.
The cigarette that makes is carried out sensory quality assessment, and the result is as shown in the table:
Above content is to combine concrete preferred implementation to the further explain that the present invention did, and can not assert that practical implementation of the present invention is confined to these explanations.For the those of ordinary skill of technical field under the present invention, under the prerequisite that does not break away from the present invention's design, can also make some simple deduction or replace, all should be regarded as belonging to protection scope of the present invention.
Claims (7)
1. the preparation method of a Stigma Maydis extract is characterized in that, comprises the steps:
Become to stick with paste step: Stigma Maydis is pulverized, added water furnishing mashed prod;
Fermentation step: add fermented bacterium at this mashed prod, ferment, obtain tunning, said fermented bacterium adopts yeast saccharomyces cerevisiae, and the inoculum size of said yeast saccharomyces cerevisiae is every gram Stigma Maydis 1 * 10
3～1 * 10
6Cfu, in the said fermentation step, temperature is 28 to 40 ℃, continues 24 to 72 hours;
The lixiviate step: in this tunning, add organic solvent, carry out lixiviate, to obtain corresponding Stigma Maydis extract, said organic solvent selects to adopt ethanol, acetone or Ucar 35, perhaps above-mentioned three's the aqueous solution; The weight of the said organic solution that is added for 4 to 10 times of said Stigma Maydis weight, is under 55 to 110 ℃ in temperature, and extraction time continues 2 to 8 hours.
2. the preparation method of Stigma Maydis extract as claimed in claim 1; It is characterized in that said one-tenth is stuck with paste in the step, pulverizes after the said Stigma Maydis drying; The particle diameter of its pulverized particles is 250 to 830 μ m, and the amount that adds entry then is 2 to 5 times of said Stigma Maydis weight.
3. the preparation method of Stigma Maydis extract as claimed in claim 1 is characterized in that, also comprises:
Filtration step: after the lixiviate step, this Stigma Maydis extract is filtered, to obtain corresponding filtrating, adopt the method for screen filtration, this mesh size is 38 to 58 μ m;
Enrichment step: concentrate for said filtrating, further obtain 0.2 to 2 times, the spissated Stigma Maydis extract of said Stigma Maydis weight.
4. the preparation method of Stigma Maydis extract as claimed in claim 3 is characterized in that,
In the said enrichment step, concentrated pressure is 0.8 * 10
4To 1 * 10
5Pa, thickening temperature are 40 ℃～100 ℃.
5. the Stigma Maydis extract that the described preparation method of arbitrary claim is obtained in employing such as the claim 1 to 4.
6. the method for use to Stigma Maydis extract as claimed in claim 5 is characterized in that, comprises the steps:
Dilution step: said Stigma Maydis extract is added organic diluting solvent get into dilution, obtain diluent, said organic diluting solvent selects to adopt Ucar 35, sorbyl alcohol, Xylitol or glycerine, perhaps above four the aqueous solution, and extension rate is 5 to 20 times;
Spray step: said diluent is sprayed the tobacco leaf surface in flue-cured tobacco.
7. the method for use of Stigma Maydis extract as claimed in claim 6 is characterized in that, said spraying in the step, and the amount of spraying of said diluent accounts for its flue-cured tobacco weight that sprays 0.1% to 1.0%.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102698105A (en) * | 2012-06-13 | 2012-10-03 | 黑龙江省祖研北药科技开发有限责任公司 | Preparation method for corn silk leavening |
| CN102688403A (en) * | 2012-06-13 | 2012-09-26 | 黑龙江省祖研北药科技开发有限责任公司 | Preparation method of corn stigma fermented product with functions of alleviating hangover and protecting liver |
| CN103695178A (en) * | 2012-09-27 | 2014-04-02 | 朱大恒 | Carrot extract and its application in tobaccos |
| CN107981406B (en) * | 2018-01-10 | 2020-04-24 | 湖北中烟工业有限责任公司 | Application method of corn stigma in paper-making reconstituted tobacco |
| CN110305735A (en) * | 2019-07-17 | 2019-10-08 | 广西中烟工业有限责任公司 | It a kind of preparation of corn tobacco aromaticss and its is applied in tobacco |
| CN114181975B (en) * | 2022-01-24 | 2023-10-24 | 黑龙江八一农垦大学 | Multi-strain composite fermentation method for fresh and tender corn silk |
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| CN1382484A (en) * | 2002-05-23 | 2002-12-04 | 刘卫国 | Corn tassel injection and its preparing process |
| CN1742950A (en) * | 2004-09-01 | 2006-03-08 | 闵伟红 | Chinese medicine preparation made from corn stigma and preparing method |
| CN101444599A (en) * | 2008-11-28 | 2009-06-03 | 长春中医药大学 | Corn silk extract and preparation method thereof and application thereof in preparing drugs for treating gout |
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| CN1382484A (en) * | 2002-05-23 | 2002-12-04 | 刘卫国 | Corn tassel injection and its preparing process |
| CN1742950A (en) * | 2004-09-01 | 2006-03-08 | 闵伟红 | Chinese medicine preparation made from corn stigma and preparing method |
| CN101444599A (en) * | 2008-11-28 | 2009-06-03 | 长春中医药大学 | Corn silk extract and preparation method thereof and application thereof in preparing drugs for treating gout |
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Application publication date: 20110615 Assignee: Shenzhen Haoyu Biological Technology Co. Ltd. Assignor: Bodun Perfume Co., Ltd., Shenzhen Contract record no.: 2017440020033 Denomination of invention: Method for preparing corn stigma extract, extract and using method of extract Granted publication date: 20120822 License type: Common License Record date: 20170510 |