CN111466609A - Preparation method of momordica grosvenori extract for treating cigarettes by using enzyme preparation - Google Patents
Preparation method of momordica grosvenori extract for treating cigarettes by using enzyme preparation Download PDFInfo
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- CN111466609A CN111466609A CN202010366882.9A CN202010366882A CN111466609A CN 111466609 A CN111466609 A CN 111466609A CN 202010366882 A CN202010366882 A CN 202010366882A CN 111466609 A CN111466609 A CN 111466609A
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- momordica grosvenori
- enzyme preparation
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- extract
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
Abstract
The invention discloses a preparation method of a cigarette momordica grosvenori extract by using an enzyme preparation, which comprises the following steps: crushing the momordica grosvenori to 20-60 meshes; adding a complex enzyme preparation with the mass ratio of 20-50% of the momordica grosvenori, and carrying out enzymolysis reaction for 2-7 h at the temperature of 30-40 ℃; carrying out high-temperature treatment at 105-120 ℃ to inactivate enzymes in the enzyme preparation; adding 50-90% ethanol aqueous solution into the enzyme preparation-treated fructus momordicae according to the material-to-liquid ratio of 1: 4-1: 10, and performing reflux extraction twice at 40-80 ℃ for 1-6 hours each time; and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby preparing the cigarette fructus momordicae extract with the relative density of 1.20-1.36. The momordica grosvenori extract obtained by the enzyme preparation treatment of the invention is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the comfortable sensation of the throat is improved, and the blank of the prior art that the momordica grosvenori is applied to the field of tobacco flavors and fragrances is filled.
Description
Technical Field
The invention relates to the technical field of preparation of extracts for cigarettes, in particular to a preparation method and application of a momordica grosvenori extract for treating cigarettes by using an enzyme preparation.
Background
In tobacco products, the aroma and the smoking taste of tobacco are important, and the addition of spices is a key link in the cigarette technology. The flavor for tobacco is divided into chemical synthetic flavor and natural flavor, and in recent years, natural plant flavor is a hot point for research and development because of its Chinese characteristics, natural harmlessness, easily available raw materials and the like. The application of the biological enzymolysis process to the production and preparation of the essences and flavors is also an effective means for improving the product style and the cigarette aroma quality.
Momordica grosvenori, a fruit of perennial vine plants of the Cucurbitaceae family. The hybrid fruits are called as the fruits of Lahidrosis, false balsam pear, Momordica grosvenori, Stephania sinica Diels, Japanese cayenne root and rhizome, and are known as the fruits of Hydnocarpus by people, and the leaves and the heart of the hybrid fruits are shaped like the male and female plants, blossom in summer and bear fruits in autumn. The momordica grosvenori is a famous and precious local special product of Guilin and is one of the first approved medicinal and edible materials in China. Fructus Siraitiae Grosvenorii has high nutritive value, and is rich in vitamins, glycoside, fructose, glucose, protein, and lipid. Fructus momordicae is sweet in taste and cool in nature, enters lung and large intestine channels, has the effects of moistening lung to arrest cough, promoting the production of body fluid to quench thirst, is suitable for lung heat or cough with dryness of lung, pertussis, thirst caused by fluid injury due to summer heat and the like, and also has the effect of relaxing bowel.
The fructus Siraitiae Grosvenorii extract can be added into cigarette to enrich tobacco fragrance and moisten smoke. However, the fructus momordicae extract obtained by the conventional extraction method inevitably contains macromolecular substances such as protein, starch, cellulose and the like, so that the fructus momordicae extract has a negative effect of great irritation when added into cigarettes.
The compound enzyme preparation is applied to extracting cigarette fructus momordicae extractum, macromolecular substances such as starch, protein, cellulose and the like can be hydrolyzed into micromolecular substances such as monosaccharide, polypeptide, amino acid and the like, micromolecular compounds with aroma characteristics in the fructus momordicae are promoted to be synthesized, converted and accumulated, the negative effects of the macromolecular substances on cigarettes can be eliminated, and the effects of increasing faint scent note and elegant feeling in the cigarettes can be achieved.
The applicant finds that the reaction of the biological enzyme has high specificity in the enzyme treatment process, so that the degradation of macromolecular components of the momordica grosvenori is single when the momordica grosvenori is treated by using a single enzyme preparation, the effect is sheet-shaped, and the best effect can be achieved by the synergistic effect of a plurality of enzymes when the quality of the momordica grosvenori extract for cigarettes is improved.
At present, no report is found yet on the application of an extract prepared by carrying out enzymolysis on momordica grosvenori through a composite biological enzyme preparation in the field of tobacco flavors and fragrances.
Disclosure of Invention
The invention provides a preparation method of a compound enzyme preparation for treating a momordica grosvenori extract for cigarettes, aiming at the defect that the momordica grosvenori extract for cigarettes, which is obtained by a conventional extraction method, contains macromolecular substances such as protein, starch, cellulose and the like and has large irritation when being added into cigarettes.
In order to realize the purpose, the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) crushing the momordica grosvenori to 20-60 meshes;
(2) adding a mixed enzyme preparation accounting for 20-50% of the mass of the momordica grosvenori, and carrying out enzymolysis reaction for 2-7 hours at 40-55 ℃;
(3) carrying out high-temperature treatment at 105-120 ℃ to inactivate enzymes in the enzyme preparation;
(4) adding 50-90% ethanol aqueous solution into the enzyme preparation-treated fructus momordicae according to the material-to-liquid ratio of 1: 4-1: 10, and performing reflux extraction twice at 40-80 ℃ for 1-6 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby preparing the cigarette fructus momordicae extract with the relative density of 1.20-1.36.
The complex enzyme preparation in the step (2) comprises α -amylase, cellulase, laccase, pectinase, flavourzyme, enzyme activity enhancer and deionized water, and the formula is shown in the table 1:
TABLE 1 formulation of Complex enzyme preparation
The invention has the following beneficial effects:
(1) according to the invention, through screening and optimized combination of single enzymes, a synergistic catalytic effect among different enzymes is realized, and a compound enzyme preparation capable of improving the quality of the cigarette fructus momordicae extract is obtained.
(2) The cigarette momordica grosvenori extract obtained by treatment of the complex enzyme preparation has mellow fragrance and good fluidity, and can soften and smooth smoke, increase sweetness, reduce irritation and improve the comfortable feeling of the throat when being added into tobacco shreds according to a certain proportion.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, and then the extract is prepared, so that the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is filled.
Detailed Description
The present invention is further illustrated in detail by the following specific examples:
example 1: the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) crushing the momordica grosvenori to 20 meshes;
(2) adding a compound enzyme preparation (the formula of the enzyme preparation is α -amylase 0.1%, cellulase 1.0%, laccase 0.1%, pectinase 0.5%, flavourzyme 1.5%, enzyme activity enhancer 20%, and deionized water 76.8%) 50% by mass of the fructus momordicae, and performing enzymolysis reaction at 40 ℃ for 4 hours;
(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 70% ethanol aqueous solution according to the material-liquid ratio of 1:5, and performing reflux extraction twice at 60 ℃ for 3 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.32.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, then the extract is prepared, and the obtained momordica grosvenori extract is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the throat comfort is improved, and the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is made up.
Example 2: the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) crushing the momordica grosvenori to 40 meshes;
(2) adding a complex enzyme preparation (the formula of the enzyme preparation is α -amylase 1.0%, cellulase 1.5%, laccase 0.2%, pectinase 0.8%, flavourzyme 1.5%, enzyme activity enhancer 20%, and deionized water 75%) 40% by mass of fructus Siraitiae Grosvenorii, and performing enzymolysis at 45 deg.C for 5 hr;
(3) treating at 115 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 60% ethanol water solution according to the material-liquid ratio of 1:6, and performing reflux extraction twice at 50 ℃ for 4 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.28.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, then the extract is prepared, and the obtained momordica grosvenori extract is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the throat comfort is improved, and the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is made up.
Example 3: the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) crushing the momordica grosvenori to 50 meshes;
(2) adding a complex enzyme preparation (the formula of the enzyme preparation is α -amylase 0.5%, cellulase 2.0%, laccase 0.15%, pectinase 0.8%, flavourzyme 2.0%, enzyme activity enhancer 20%, and deionized water 74.55%) 35% by mass of fructus momordicae, and performing enzymolysis reaction at 55 ℃ for 4 h;
(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 80% ethanol water solution according to the material-liquid ratio of 1:5, and performing reflux extraction twice at 50 ℃ for 4 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.30.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, then the extract is prepared, and the obtained momordica grosvenori extract is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the throat comfort is improved, and the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is made up.
Example 4: the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) pulverizing fructus Siraitiae Grosvenorii to 60 mesh;
(2) adding a complex enzyme preparation (the formula of the enzyme preparation is α -amylase 0.8%, cellulase 1.0%, laccase 0.2%, pectinase 0.8%, flavourzyme 1.8%, enzyme activity enhancer 20%, and deionized water 75.4%) which accounts for 40% of the mass of the momordica grosvenori, and carrying out enzymolysis reaction at 50 ℃ for 3 hours;
(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 80% ethanol water solution according to the material-liquid ratio of 1:5, and performing reflux extraction twice at 60 ℃ for 3 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.25.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, then the extract is prepared, and the obtained momordica grosvenori extract is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the throat comfort is improved, and the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is made up.
Example 5: the preparation method of the cigarette momordica grosvenori extract comprises the following steps:
(1) crushing the momordica grosvenori to 30 meshes;
(2) adding a compound enzyme preparation (the formula of the enzyme preparation is α -amylase 0.8%, cellulase 1.2%, laccase 0.2%, pectinase 0.8%, flavourzyme 2.0%, enzyme activity enhancer 20%, and deionized water 75%) 50% by mass of fructus momordicae, and performing enzymolysis reaction at 40 ℃ for 6 h;
(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 60% ethanol water solution according to the material-liquid ratio of 1:6, and performing reflux extraction twice at 60 ℃ for 5 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.25.
According to the invention, the momordica grosvenori is subjected to biological enzymolysis, then the extract is prepared, and the obtained momordica grosvenori extract is added into cigarettes, so that the smoke can be softened and refined, the sweetness is increased, the stimulation is reduced, the throat comfort is improved, and the blank of the momordica grosvenori in the prior art in the field of tobacco essence and flavor is made up.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (3)
1. A preparation method of a cigarette siraitia grosvenorii extract by enzyme preparation treatment is characterized by comprising the following steps:
(1) crushing the momordica grosvenori to 20-60 meshes;
(2) adding a complex enzyme preparation with the mass ratio of 20-50% of the momordica grosvenori, and carrying out enzymolysis reaction for 2-7 h at the temperature of 40-55 ℃;
(3) carrying out high-temperature treatment at 105-120 ℃ to inactivate enzymes in the enzyme preparation;
(4) adding 50-90% ethanol aqueous solution into the enzyme preparation-treated fructus momordicae according to the material-to-liquid ratio of 1: 4-1: 10, and performing reflux extraction twice at 40-80 ℃ for 1-6 hours each time;
(5) and combining the two extracting solutions, precipitating, filtering, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby preparing the cigarette fructus momordicae extract with the relative density of 1.20-1.36.
2. The preparation method of claim 1, wherein the complex enzyme preparation in step (2) comprises α -amylase, cellulase, laccase, pectinase, flavourzyme, enzyme activity enhancer and deionized water, and the formula is shown in Table 1:
TABLE 1 formulation of Complex enzyme preparation
3. The method of claim 1, comprising the steps of:
(1) crushing the momordica grosvenori to 50 meshes;
(2) adding a complex enzyme preparation accounting for 35 percent of the mass of the momordica grosvenori into the mixture for enzymolysis reaction for 4 hours at the temperature of 55 ℃, wherein the enzyme preparation comprises α -amylase 0.5 percent, cellulase 2.0 percent, laccase 0.15 percent, pectinase 0.8 percent, flavourzyme 2.0 percent, enzyme activity enhancer 20 percent and deionized water 74.55 percent;
(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;
(4) adding the momordica grosvenori treated by the enzyme preparation into 80% ethanol water solution according to the material-liquid ratio of 1:5, and performing reflux extraction twice at 50 ℃ for 4 hours each time;
(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.30.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113892675A (en) * | 2021-10-29 | 2022-01-07 | 广西中烟工业有限责任公司 | Lung moistening and cough relieving fructus momordicae and Chinese date compound flavor bagged mouth containing cigarette |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768515A (en) * | 2010-01-13 | 2010-07-07 | 周煜航 | Preparation method and application of natural extractive |
| CN108142988A (en) * | 2017-12-08 | 2018-06-12 | 湖北中烟工业有限责任公司 | A kind of preparation method of Fructus Monordicae extract for flavouring humectation |
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2020
- 2020-04-30 CN CN202010366882.9A patent/CN111466609A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768515A (en) * | 2010-01-13 | 2010-07-07 | 周煜航 | Preparation method and application of natural extractive |
| CN108142988A (en) * | 2017-12-08 | 2018-06-12 | 湖北中烟工业有限责任公司 | A kind of preparation method of Fructus Monordicae extract for flavouring humectation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113892675A (en) * | 2021-10-29 | 2022-01-07 | 广西中烟工业有限责任公司 | Lung moistening and cough relieving fructus momordicae and Chinese date compound flavor bagged mouth containing cigarette |
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