CN108142988A - A kind of preparation method of Fructus Monordicae extract for flavouring humectation - Google Patents
A kind of preparation method of Fructus Monordicae extract for flavouring humectation Download PDFInfo
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- CN108142988A CN108142988A CN201711291789.0A CN201711291789A CN108142988A CN 108142988 A CN108142988 A CN 108142988A CN 201711291789 A CN201711291789 A CN 201711291789A CN 108142988 A CN108142988 A CN 108142988A
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- Prior art keywords
- mycelium
- fermentation
- ganoderma lucidum
- extract
- siraitia grosvenorii
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- 239000000284 extract Substances 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims abstract description 32
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 30
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 30
- 238000000855 fermentation Methods 0.000 claims abstract description 28
- 230000004151 fermentation Effects 0.000 claims abstract description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 20
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- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 235000012015 potatoes Nutrition 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims 1
- 238000002386 leaching Methods 0.000 claims 1
- 235000019504 cigarettes Nutrition 0.000 abstract description 20
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 abstract description 8
- 239000003546 flue gas Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 239000000796 flavoring agent Substances 0.000 abstract description 7
- 235000019634 flavors Nutrition 0.000 abstract description 7
- 230000007794 irritation Effects 0.000 abstract description 4
- 244000185386 Thladiantha grosvenorii Species 0.000 description 24
- 235000019441 ethanol Nutrition 0.000 description 9
- 239000003205 fragrance Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 241000233866 Fungi Species 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 244000061176 Nicotiana tabacum Species 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 239000000654 additive Substances 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108010029541 Laccase Proteins 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 102000006995 beta-Glucosidase Human genes 0.000 description 3
- 108010047754 beta-Glucosidase Proteins 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 229930189775 mogroside Natural products 0.000 description 3
- 239000002304 perfume Substances 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 241000205585 Aquilegia canadensis Species 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
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- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 description 1
- OYHQOLUKZRVURQ-UHFFFAOYSA-N 9,12-Octadecadienoic Acid Chemical class CCCCCC=CCC=CCCCCCCCC(O)=O OYHQOLUKZRVURQ-UHFFFAOYSA-N 0.000 description 1
- 240000000011 Artemisia annua Species 0.000 description 1
- 235000001405 Artemisia annua Nutrition 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000522254 Cassia Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- RDMQPKIDHAFXKA-JNORPAGFSA-N Ganoderic Acid Am1 Chemical compound C([C@@]12C)C[C@H](O)C(C)(C)[C@@H]1CC(=O)C1=C2C(=O)C[C@]2(C)[C@@H]([C@@H](CC(=O)CC(C)C(O)=O)C)CC(=O)[C@]21C RDMQPKIDHAFXKA-JNORPAGFSA-N 0.000 description 1
- 229930182735 Ganoderic acid Natural products 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
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- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 240000002924 Platycladus orientalis Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- -1 aldoketones Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
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- 125000003118 aryl group Chemical group 0.000 description 1
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- 238000002485 combustion reaction Methods 0.000 description 1
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- 239000000706 filtrate Substances 0.000 description 1
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- 230000002538 fungal effect Effects 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
- A24B15/303—Plant extracts other than tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
Abstract
The present invention provides a kind of preparation method of Fructus Monordicae extract for flavouring humectation.Ganoderma lucidum mycelium is specifically first carried out to the activation of strain, and the ganoderma lucidum mycelium liquid that can carry out fermentation reaction is obtained after shake culture；Then Siraitia grosvenorii is crushed, and add water mixed high-voltage steam sterilizing after aseptically, add in ganoderma lucidum mycelium liquid, it is 58 in pH, 28 30 DEG C of temperature, glossy ganoderma mycelium fermentation Siraitia grosvenorii product is obtained by the reaction to carry out liquid fermentation interaction under conditions of 300 600L/h, 150 180r/min of mixing speed in air intake, the tunning then is added in the extraction of ethanol solution slightly boiling, it is the Fructus Monordicae extract that extracting solution liquid is concentrated into medicinal extract shape.The extract of glossy ganoderma mycelium fermentation Siraitia grosvenorii in the present invention is added in cigarette, not only has effects that flavouring humectation, and by fermentation after, further enrich the type and content of flavor component, enhance the mildness of flue gas, reduce the irritation of flue gas.
Description
Technical field
The present invention relates to a kind of development and application of the novel fragrance for flavouring humectation effect, specifically from ganoderma lucidum
In mycelium under the action of own laccase and beta-glucosidase, compounding hair is carried out with Siraitia grosvenorii for the mycelium of middle extraction
Ferment obtains a kind of novel flavouring humectation product, and in cigarette after extraction.
Background technology
Early in 16th century in the discovery of America by Columbus New World, American Indian of Mexico had started to add in tobacco at that time
Orange peel oil is added to enhance fragrance.All there is the cigarette of spices and essence respectively in, Japan in 1850 in nineteen twenty in the U.S., China
In the flavouring essence for tobacco for starting the odor types such as banana, Rum occur around the 1930s, current China's additive in cigarette most flows
Capable is Chinese herb feed additive, for example the seed of Oriental arborvitae, Radix Ophiopogonis, Siraitia grosvenorii, honeysuckle, ginseng, dandelion, Chinese cassia tree etc. are with health care work(
Energy；Tea dust, honeysuckle, Fructus Forsythiae and mulberry leaf etc. have flavouring fresh-keeping function；Green tea, sweet wormwood, Radix Glycyrrhizae, Rhizoma Chuanxiong etc. have reduction tar
Function.Data from tobacco business shows that at present, more than 3,000 kinds of natural perfume material and more than 7,000 kinds of synthetic perfume collectively form
The range of choice of cigarette additive.The most important effect of cigarette additive is to increase the fragrance of cigarette and improve its mouthfeel, together
Shi Youguan researchs are also shown that harmful free radicals and the polycyclic virtue of carcinogen in certain cigarette additive cigarette capable of reducing combustion processes
The generation of hydrocarbon, so as to have the function that reducing tar and reducing harm humectation.
Fragrance material is extracted from natural plants, product is limited, while also other factors by seasonal and quantity,
As weather conditions, geographical conditions and change.On the contrary, have and be different from through the certain aromatic substances of biosynthesis using microorganism
The advantage of natural Chinese medicinal herb, for example be not easy to be influenced by natural environment and weather, it is easy to mass produce, production cost is low, week
Phase is short etc..In recent years, it is production fragrance object using the specificity of its biosynthesis by the culture to rare edible and medicinal fungi
One of most promising source of matter.The growth and development of most medicinal fungus and its micro-ecological environment are closely related, specific
Under the conditions of could form sexual system's fructification, artificial cultivation is extremely difficult, cultivates so far successful less than hundred kinds.As a result wild money is caused
Source it is rare, so as to the critical bottleneck utilized as fungal resources.Research shows that is cloned from fungus fructification is asexual
It is that brood body-mycelium has the main effect component similar with fungus, therefore, obtains fungus using mycelium fermentation Chinese herbal medicine and live
Property extract become current fungus research and development an important directions, also as cigarette additive exploitation new way.
Ganoderic acid, the saponins contained in traditional Chinese medicine ganoderma lucidum can inhibit tumour, antiviral, liver protection and anti-aging
Deng.But artificial cultivation growth cycle is long, low output, and wild resource is in great shortage, it is impossible to meet needs.It is obtained using mycelium submerged fermentation
The extract arrived has the composition similar to cultivating ganoderma, and production time from half a year shortens to several days.
Invention content
The purpose of the present invention is provide a kind of glossy ganoderma mycelium fermentation sieve for flavouring humectation effect according to the prior art
The preparation method of Chinese fruit product extract, and the extract is applied in cigarette, not only there is flavouring humectation, but also
The preparation method is simple, quality controllable, easy industrialized production.
The present invention is achieved through the following technical solutions：A kind of preparation side of Fructus Monordicae extract for flavouring humectation
Method, it is characterised in that be as follows：
(1) preparation of ganoderma lucidum mycelium liquid：Ganoderma lucidum mycelium is placed in potato sucrose agar medium, and (PSA is cultivated
Base) in 28 DEG C of cultures activation for carrying out strain in 7 days, the mycelium is inoculated into potato culture medium (PDY culture mediums) after activation, is put
In on shaking table, adjusting rotating speed is 160r/min, and temperature was 28.5 DEG C of shake cultures after 7 days, obtained the spirit that can carry out fermentation reaction
Camphorata mycelium liquid；
(2) preparation of glossy ganoderma mycelium fermentation Siraitia grosvenorii product：Siraitia grosvenorii shell is torn to bits, seed is separated, by arhat
Fruit and water are according to weight ratio 1:6-1:10 ratio is mixed, after mixing at 121 DEG C high pressure steam sterilization 30min, treat it
After natural cooling, under aseptic condition, ganoderma lucidum mycelium liquid that step (1) is obtained, according to Siraitia grosvenorii mixture weight
The 1 of ratio:10-1:15 are mixed, and 3-6 times of water of total weight of the mixture is added in after mixing, are 5-8, temperature 28-30 in pH
DEG C, air intake reacts 6- to carry out liquid fermentation interaction under conditions of 300-600L/h, mixing speed 150-180r/min
10 days, obtain glossy ganoderma mycelium fermentation Siraitia grosvenorii product；
(3) extraction of tunning：The tunning that step (2) obtains is added in into concentration according to 3-6 times of its weight
70% ethanol solution keeps slight boiling condition 1-1.5h under the conditions of 85-90 °, takes out supernatant, adds fermentate weight 6-
10 times of 70% ethanol solution, continues to extract, and keeps slightly boiling 0.5-1h, takes out supernatant, and it is dense to merge extracting solution twice
It is the Fructus Monordicae extract to be reduced to medicinal extract shape.
The present invention preferably technical solution：Water described in above-mentioned steps is aqua sterilisa, pure water or distilled water.
The present invention preferably technical solution：PSA culture mediums in the step (1) are by 200g peeled potatoes, 20g sugarcanes
The culture medium that sugar, 25g agar and 1000ml water are formed at 121 DEG C after high pressure steam sterilization 30min after mixing.
The present invention preferably technical solution：PDY culture mediums in the step (1) are by 200g peeled potatoes, 25g Portugals
The culture medium that high pressure steam sterilization 35min is formed at 115 DEG C after grape sugar and 1000ml water mixings.
The present invention preferably technical solution：Ganoderma lucidum mycelium in the step (1) is the ganoderma lucidum mycelium directly bought
Or the ganoderma lucidum mycelium that lucidum strain routine culture obtains, preservation under the conditions of placing it in -18 DEG C before the use are using it
Before, defrosting is taken out to room temperature.The ganoderma lucidum mycelium most preferably uses Wild ganoderma mycelium.
Beneficial effects of the present invention：
(1) present invention obtains that Siraitia grosvenorii extractive from fermentative preparation process is simple, of low cost, production efficiency is high, can carry out
Large-scale industrial production；
(2) extract of the glossy ganoderma mycelium fermentation Siraitia grosvenorii in the present invention is added in cigarette, is not only protected with flavouring
The effect of profit, and by fermentation after, further enrich the type and content of flavor component, enhance the mildness of flue gas, drop
The low irritation of flue gas.
Specific embodiment
With reference to embodiment and contrast experiment, the present invention will be further described.
Ganoderma lucidum mycelium liquid specifically obtains in accordance with the following methods in following embodiment：By the Ganoderma Lucidum of -18 DEG C of preservations
Filament takes out, and thaws to room temperature, which is placed in potato sucrose agar medium (PSA culture mediums) in 28 DEG C of cultures
The activation for carrying out strain in 7 days, is inoculated into potato culture medium (PDY culture mediums) by the mycelium after activation, is placed on shaking table, adjusts
Rotating speed is 160r/min, and temperature was 28.5 DEG C of shake cultures after 7 days, obtained the ganoderma lucidum mycelium liquid that can carry out fermentation reaction；
The ganoderma lucidum mycelium is the ganoderma lucidum mycelium that the ganoderma lucidum mycelium directly bought or lucidum strain routine culture obtain, and is made
With preservation under the conditions of placing it in -18 DEG C before, before the use, defrosting is taken out to room temperature.
Embodiment 1, a kind of Fructus Monordicae extract for flavouring humectation are prepared by following methods：
(1) Siraitia grosvenorii shell is torn to bits, seed is separated, by Siraitia grosvenorii and water according to weight ratio 1:8 ratio is mixed
Close, after mixing at 121 DEG C high pressure steam sterilization 30min, after its natural cooling, under aseptic condition, by above-mentioned Ganoderma Lucidum
Filament liquid is with Siraitia grosvenorii mixture according to the 1 of weight ratio:12 are mixed, and after mixing, add in 5 times of total weight of the mixture
Water is 5-8 in pH, and 29 DEG C of temperature, air intake is carries out liquid fermentation under conditions of 450L/h, mixing speed 160r/min
Interaction is reacted 8 days, obtains glossy ganoderma mycelium fermentation Siraitia grosvenorii product；
(2) extraction of tunning：The tunning that step (1) obtains is added in content according to 5 times of its weight is
70% ethyl alcohol keeps slight boiling condition 1-1.5h at 90 °, takes out supernatant, adds 70% second of 8 times of fermentate weight
Alcohol continues to extract, and keeps slightly boiling 1h, takes out supernatant, merges extracting solution twice and is concentrated into medicinal extract shape.
The extract obtained in embodiment 1 is 165.9mg/ by experiment, yield of extract 0.277g/g, general flavone yield
G, mogroside yield are 64.7mg/g, are added to cigarette perfume in cigarette and enrich, and fragrance is sent out thoroughly, and irritation reduces.
Embodiment 2, a kind of Fructus Monordicae extract for flavouring humectation are prepared by following methods：
(1) Siraitia grosvenorii shell is torn to bits, seed is separated, by Siraitia grosvenorii and water according to weight ratio 1:6 ratio is mixed
Close, after mixing at 121 DEG C high pressure steam sterilization 30min, after its natural cooling, under aseptic condition, by above-mentioned Ganoderma Lucidum
Filament liquid is with Siraitia grosvenorii mixture according to the 1 of weight ratio:10 are mixed, and after mixing, add in 4 times of total weight of the mixture
Water is 5-8 in pH, and 28 DEG C of temperature, air intake is carries out liquid fermentation under conditions of 400L/h, mixing speed 150r/min
Interaction is reacted 6 days, obtains glossy ganoderma mycelium fermentation Siraitia grosvenorii product；
(2) extraction of tunning：The tunning that step (1) obtains is added in content according to 3 times of its weight is
70% ethyl alcohol keeps slight boiling condition 1h at 85 °, takes out supernatant, adds 70% ethyl alcohol of 6 times of fermentate weight, after
It is continuous to extract, and keep slightly boiling 0.5h, supernatant is taken out, merges extracting solution twice and is concentrated into medicinal extract shape.
The extract obtained in embodiment 2 is 153.2mg/ by experiment, yield of extract 0.265g/g, general flavone yield
G, mogroside yield are 60.5mg/g.
Embodiment 3, a kind of Fructus Monordicae extract for flavouring humectation are prepared by following methods：
(1) Siraitia grosvenorii shell is torn to bits, seed is separated, by Siraitia grosvenorii and water according to weight ratio 1:10 ratio is mixed
Close, after mixing at 121 DEG C high pressure steam sterilization 30min, after its natural cooling, under aseptic condition, by above-mentioned Ganoderma Lucidum
Filament liquid is with Siraitia grosvenorii mixture according to the 1 of weight ratio:15 are mixed, and after mixing, add in 6 times of total weight of the mixture
Water is 5-8 in pH, and 30 DEG C of temperature, air intake is carries out liquid fermentation under conditions of 600L/h, mixing speed 180r/min
Interaction is reacted 10 days, obtains glossy ganoderma mycelium fermentation Siraitia grosvenorii product；
(2) extraction of tunning：The tunning that step (1) obtains is added in content according to 6 times of its weight is
70% ethyl alcohol keeps slight boiling condition 1.5h at 90 °, takes out supernatant, adds 70% ethyl alcohol of 10 times of fermentate weight,
Continue to extract, and keep slightly boiling 1h, take out supernatant, merge extracting solution twice and be concentrated into medicinal extract shape.
The extract obtained in embodiment 3 is 171.6mg/ by experiment, yield of extract 0.293g/g, general flavone yield
G, mogroside yield are 69.4mg/g.
Embodiment 4:In the method for perfuming cigarette, the extractive from fermentative prepared in embodiment 1 is diluted to 70% ethyl alcohol
5% flavoring solution is uniformly sprayed at by tobacco metering with 0.00%, 0.01%, 0.02%, 0.03%, 0.05% respectively
In the blank pipe tobacco of high, medium and low three kinds of class, cigarette is rolled into, under conditions of relative humidity 60 ± 2%, 22 ± 1 DEG C of temperature,
More than 48h is balanced, carries out sensory evaluation according to GB5606.4-2005 methods, evaluation result refers to table 1；
From table 1 it follows that glossy ganoderma mycelium fermentation Fructus Monordicae extract in low and middle-grade cigarette can with soft flue gas,
Reduce irritation, while have certain flavouring effect, particularly when dosage is 0.03%-0.05%, the soft degree of flue gas and
Conglobation property improvement is obvious, but with the increase of dosage, the property sent out has reduction thoroughly for concentration and fragrance.It is but soft in high-grade cigarette
Unobvious are acted on flue gas, this may be finer and smoother soft related with high-grade cigarette flue gas itself.
Sensory evaluation of the 1 glossy ganoderma mycelium fermentation Fructus Monordicae extract of table in cigarette
Technical advance in order to further illustrate the present invention, we comment the biological property of ganoderma lucidum mycelium
Valency, evaluation result are shown in Table 2.
2 biological property of table is evaluated
The speed of growth of ganoderma lucidum is very fast as can be seen from the above table, is highly suitable for industrialized production.And beta-glucosidase
The enzymatic activity of enzyme and laccase is also very high, and beta-glucosidase is enzyme common in mycelium, can be by flavor precursor glucosides
Class compound is decomposed into various fragrance matters, and laccase is also a kind of important enzyme in mycelium, can be decomposed in Siraitia grosvenorii
Lignocellulosic destroys cell wall structure, so as to improve the extracted amount of flavor component in Siraitia grosvenorii.
Technical advance in order to further illustrate the present invention is respectively adopted in embodiment the front and rear Siraitia grosvenorii that ferments and walks
Suddenly after the extracting method of (2) extracts, and the GC/MS that flavor component is carried out to extract is analyzed, specific as follows：
Extract medicinal extract is distilled into water dissolutions with 10 grams for 1 gram, is extracted with 10ml dichloromethane, first hunted leak before extraction,
After deflate, shake about 1min, stand 20min., extract 3 times, combined dichloromethane extract liquor, be concentrated into 5ml, add in 5 grams it is anhydrous
Sodium sulphate is placed for 24 hours, and with filtering with microporous membrane, filtrate is stored in chromatogram bottle in 0 DEG C of -4 DEG C of preservation, pending Gc-mss.
GC conditions：DB-WAX capillary columns (30m × 0.25 μm of 250 μ m)；Carrier gas is He, flow 1.0ml/
min；Split ratio 5:1；1 μ L of sample size.
Temperature program：220 DEG C of injector temperature, 80 DEG C of initial temperature rise to 200 DEG C, then with 5 DEG C/min with 10 DEG C/min
290 DEG C are risen to, finally stops 18min.
Mass Spectrometry Conditions：EI sources, 230 DEG C of temperature, mass spectrometer interface temperature are 280 DEG C, electron energy 70eV, quality of scanning range
For 40~500m/z, retrieval spectrum library NIST05.
The type and content of flavor component before and after the fermentation of 3 Siraitia grosvenorii of table
From table 3 it can be seen that the type of Fructus Monordicae extract flavor component having through everfermentation significantly increases, content also has
Changed, not only remain the ingredients such as original hydro carbons, aldoketones, esters, also add 9,12- octadecadienoic acids, 4- hydroxyls
The compounds such as benzyl carbinol.
Claims (5)
1. the preparation method of a kind of Fructus Monordicae extract for flavouring humectation, it is characterised in that be as follows：
(1) preparation of ganoderma lucidum mycelium liquid：Ganoderma lucidum mycelium is placed in PSA cultures and carries out strain within 7 days based on 28 DEG C of cultures
Activation, the mycelium is inoculated into PDY culture mediums after activation, is placed on shaking table, adjustings rotating speed be 160r/min, temperature 28.5
DEG C shake culture obtains the ganoderma lucidum mycelium liquid that can carry out fermentation reaction after 7 days；
(2) preparation of glossy ganoderma mycelium fermentation Siraitia grosvenorii product：Siraitia grosvenorii shell is torn to bits, seed is separated, by Siraitia grosvenorii and
Water is according to weight ratio 1:6-1:10 ratio is mixed, after mixing at 121 DEG C high pressure steam sterilization 30min, treat its nature
After cooling, under aseptic condition, ganoderma lucidum mycelium liquid that step (1) is obtained, according to Siraitia grosvenorii mixture weight than
1:10-1:15 are mixed, and 3-6 times of water of total weight of the mixture is added in after mixing, are 5-8 in pH, 28-30 DEG C of temperature is empty
Gas intake is reacted 6-10 days to carry out liquid fermentation interaction under conditions of 300-600L/h, mixing speed 150-180r/min,
Obtain glossy ganoderma mycelium fermentation Siraitia grosvenorii product；
(3) extraction of tunning：The tunning that step (2) obtains is added in into concentration 70% according to 3-6 times of its weight
Ethanol solution keeps slight boiling condition 1-1.5h under the conditions of 85-90 °, takes out supernatant, adds 6-10 times of fermentate weight
70% ethanol solution continues to extract, and keeps slightly boiling 0.5-1h, takes out supernatant, merges extracting solution twice and is concentrated into leaching
Paste is the Fructus Monordicae extract.
2. a kind of preparation method of Fructus Monordicae extract for flavouring humectation according to claim 1, it is characterised in that：
Water described in above-mentioned steps is aqua sterilisa, pure water or distilled water.
3. a kind of preparation method of Fructus Monordicae extract for flavouring humectation according to claim 1, it is characterised in that：
PSA culture mediums in the step (1) are by 200g peeled potatoes, 20g sucrose, and 25g agar and 1000ml water are uniformly mixed
The culture medium formed after high pressure steam sterilization 30min at 121 DEG C afterwards.
4. a kind of preparation method of Fructus Monordicae extract for flavouring humectation according to claim 1, it is characterised in that：
PDY culture mediums in the step (1) be by after 200g peeled potatoes, 25g glucose and 1000ml water mixings at 115 DEG C
The culture medium that high pressure steam sterilization 35min is formed.
5. a kind of preparation method of Fructus Monordicae extract for flavouring humectation according to claim 1, it is characterised in that：
Ganoderma lucidum mycelium in the step (1) is the Ganoderma Lucidum that the ganoderma lucidum mycelium directly bought or lucidum strain routine culture obtain
Filament, preservation under the conditions of placing it in -18 DEG C before the use before the use, are taken out defrosting to room temperature.
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| CN109090702A (en) * | 2018-06-29 | 2018-12-28 | 郑州轻工业学院 | The preparation method and applications of Luo Han Guo Paste In Foods maillard reaction product |
| CN111466609A (en) * | 2020-04-30 | 2020-07-31 | 广西中烟工业有限责任公司 | Preparation method of momordica grosvenori extract for treating cigarettes by using enzyme preparation |
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