CN102010248A - Complex microorganism organic fertilizer and preparation method thereof - Google Patents

Complex microorganism organic fertilizer and preparation method thereof Download PDF

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CN102010248A
CN102010248A CN2010105180036A CN201010518003A CN102010248A CN 102010248 A CN102010248 A CN 102010248A CN 2010105180036 A CN2010105180036 A CN 2010105180036A CN 201010518003 A CN201010518003 A CN 201010518003A CN 102010248 A CN102010248 A CN 102010248A
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bacterium liquid
weight
organic fertilizer
seed
bacillus
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孙明坤
郭少萍
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a complex microorganism organic fertilizer and a preparation method thereof. The complex microorganism organic fertilizer is prepared by the following steps of: putting the following raw materials in proportion by weight into a Saccharomgces cerevasae liquid accounting for 0.5-0.8% of the weight of the raw materials, a Bacillus laterosporus liquid accounting for 0.1-0.5% of the weight of the raw materials and Bacillus megaterium liquid accounting for 0.1-0.5% of the weight of the raw materials for fermentation, wherein the raw materials comprise 1-50 parts of castor bean meal, 1-50 parts of cow or chicken dung, 1-20 parts of rice hull bran, 1-10 parts of bone meal, 1-80 parts of straws and 1-40 parts of rapeseed oil and cake in proportion by weight; and after the fermentation, adding 1-20 parts of urea, 0.5-10 parts of double superphosphate and 0.5-15 parts of potassium sulfate to prepare into the complex microorganism organic fertilizer, wherein the moisture content of the complex microorganism organic fertilizer is below 30 percent. The organic fertilizer can completely substitute a chemical fertilizer and can be used a base fertilizer or a additional fertilizer.

Description

A kind of composite microbial organic fertilizer and preparation method thereof
Technical field
The present invention relates to a kind of composite microbial organic fertilizer and preparation method thereof.
Background technology
For many years, in arviculture, the consumption of chemical fertilizer is in continuous increase, the consumption of organic fertilizer is fewer and feweri, and farm crop to the utilization ratio of chemical fertilizer only about 30%, about 70% chemical fertilizer deposition is fixed in the soil or runs off in rivers and not by crop absorption and utilization, this has caused soil compaction with rainwater, environmental pollution, rivers and underground water eutrophication are on the rise, and soil organic matter content reduces, and fertility descends, the beneficial microorganism living space diminishes in the soil, quantity reduces, farm crop disease-resistant, cold-resistant, drought-resistant ability weakens, and the generation of disease and pest is more and more serious, cause crops quality to descend, output reduces.Composite microbiological fertilizer is the extraordinary fertilizer that utilizes various agricultural organic waste materials and part mineral manure to combine.In recent years, along with people improve constantly the understanding of green food, Organic food, the enterprise whole nation of plantation green food has surpassed more than 6000 families, and product is above 15000.Wherein: crop acreage reaches 1.9 hundred million mu, accounts for 8% of national proportion of crop planting.The domestic annual sales amount of green food has reached more than 3,000 hundred million, export amount has reached more than 20 hundred million dollars, most like this plantation enterprise needs a kind of good fertilizer that can replace chemical fertilizer, because green food standard requires to reduce the usage quantity of chemical fertilizer 30%, and does not allow crop failure.Therefore, now people more and more pay attention to composite microbiological fertilizer and apply on farm crop.
Summary of the invention
First technical problem that the present invention will solve provides a kind of composite microbial organic fertilizer.This fertilizer can replace chemical fertilizer as the base manure or the use of topdressing fully.
Second technical problem that the present invention will solve provides a kind of preparation method of composite microbial organic fertilizer.
For solving the problems of the technologies described above, technical scheme that the present invention adopts is:
The invention provides a kind of composite microbial organic fertilizer, it is to insert yeast (Saccharomgces cerevasae) the bacterium liquid that accounts for raw material weight 0.5%~0.8% by following weight proportion raw material, bacillus laterosporus (Bacillus laterosporus) the bacterium liquid that accounts for raw material weight 0.1~0.5% ferments with bacillus megaterium (Bacillus megaterium) the bacterium liquid that accounts for raw material weight 0.1~0.5%, the fermentation back adds 1~20 part urea, 0.5~10 parts double superhosphate, 0.5 the composite microbial organic fertilizer that~15 parts vitriolate of tartar is made, the water content of described composite microbial organic fertilizer are below 30%;
Wherein: the raw material weight proportioning is: 1~50 part of the castor-oil plant dregs of rice, 1~50 part of ox or chicken manure, 1~20 part of rice husk chaff, 1~10 part of bone meal, 1~80 part of straw, 1~40 part of the rape oil dregs of rice.
The present invention also provides a kind of preparation method of composite microbial organic fertilizer, and this preparation method comprises the following steps:
(1) presses column weight amount umber proportioning and get raw material: 1~50 part of the castor-oil plant dregs of rice, 1~50 part of ox or chicken manure, 1~20 part of rice husk chaff, 1~10 part of bone meal, 1~80 part of straw, 1~40 part of the rape oil dregs of rice, 1~20 part in urea, 0.5~10 part of double superhosphate, 0.5~15 part of vitriolate of tartar;
(2) saccharomycetes to make fermentation: with 1~50 part the castor-oil plant dregs of rice, 1~50 part ox or chicken manure, 1~20 part rice husk chaff, 1~10 part bone meal, 1~80 part straw, 1~40 part the rape oil dregs of rice mix, access accounts for yeast (Saccharomgces cerevasae) the bacterium liquid of raw material weight 0.5%~0.8%, and after 12 hours, aerobic fermentation is carried out in ventilation, leavening temperature is controlled at 40 ℃~50 ℃, and humidity of materials is controlled at 40%~50%;
(3) bacillus laterosporus and bacillus megaterium fermentation: saccharomycetes to make fermentation 6~12 days, insert bacillus laterosporus (Bacillus laterosporus) bacterium liquid that accounts for raw material weight 0.1~0.5% and bacillus megaterium (Bacillus megaterium) the bacterium liquid that accounts for raw material weight 0.1~0.5% and carried out heap fermentation 2~3 days;
Bacillus laterosporus and bacillus megaterium are to add the later stage of fermentation, fermentation is finished before 2-3 days, and the expansion of favourable like this microorganism is decomposed, and viable count is above the standard of NY/T 798-2004.
(4) through the fertilizer after step (3) fermentation, add 1~20 part urea, 0.5~10 part double superhosphate, 0.5~15 part vitriolate of tartar mixes, and promptly gets product, and its water content is below 30%.
Described saccharomycetic cultural method is: yeast starter kind being inserted the slant activation substratum activate, is to cultivate 24~48 hours under 28 ℃~30 ℃ the condition in temperature; 5%~30% the amount of activatory yeast by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The yeast bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 24~48 hours and cultivate, and promptly gets employed yeast bacterium liquid;
The weight percent of each ingredients constitute substratum is in the described slant activation substratum: glucose 0.5%~1.0% or brown sugar (Saccharum Sinensis Roxb.) 5%~10%, peptone 0.5%~1.0%, sal epsom 0.1%~0.2%, potassium primary phosphate 0.1%~0.3%, dipotassium hydrogen phosphate 0.1%~0.5%, agar 2%, add water to 100%, adjust pH is 5.6~7.0;
The weight percent of each ingredients constitute substratum is in the described seed culture medium: glucose 0.5%~1.0% or brown sugar (Saccharum Sinensis Roxb.) 5%~10%, sal epsom 0.1%~0.2%, potassium primary phosphate 0.1%~0.3%, dipotassium hydrogen phosphate 0.1%~0.5%, add water to 100%, adjust pH is 5.6~7.0.
The cultural method of described bacillus laterosporus is: the female kind of bacillus laterosporus inserted the slant activation substratum and activated, and is to cultivate 24~48 hours under 32 ℃~35 ℃ the condition in temperature; 5%~30% the amount of activatory bacillus laterosporus by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The bacillus laterosporus bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 48~72 hours and cultivate; Promptly get employed bacillus laterosporus bacterium liquid;
The weight percent of each ingredients constitute substratum is in the described slant activation substratum: peptone 0.5%~1.0%, extractum carnis 0.3%~0.5%, sodium-chlor 0.2%~0.5%, agar 2%, add water to 100%, and adjust pH is 6.0~7.0;
The weight percent of each ingredients constitute substratum is in the described seed culture medium: bean powder 0.5%~1.0%, Semen Maydis powder 0.5%~1.0%, sodium-chlor 0.2%~0.5%, potassium primary phosphate 0.1%~0.5%, add water to 100%, and adjust pH is 6.0~7.0.
The cultural method of described bacillus megaterium is: the female kind of bacillus megaterium inserted the slant activation substratum and activated, and is to cultivate 24~48 hours under 32 ℃~35 ℃ the condition in temperature; 5%~30% the amount of activatory bacillus megaterium by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The bacillus megaterium bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 48~72 hours and cultivate; Promptly get employed bacillus megaterium bacterium liquid;
The weight percent of each ingredients constitute substratum is in the described slant activation substratum: peptone 0.5%~1.0%, extractum carnis 0.3%~0.5%, sodium-chlor 0.2%~0.5%, agar 2%, add water to 100%, and adjust pH is 6.0~7.0;
The weight percent of each ingredients constitute substratum is in the described seed culture medium: bean powder 0.5%~1.0%, Semen Maydis powder 0.5%~1.0%, sodium-chlor 0.2~0.5%, potassium primary phosphate 0.1%~0.5%, add water to 100%, and adjust pH is 6.0~7.0.
In step (4), can also add trace elements iron, zinc, copper, manganese, molybdenum, boron, magnesium and be in harmonious proportion.
Beneficial effect of the present invention:
1, composite microbiological fertilizer of the present invention can replace chemical fertilizer as the base manure or the use of topdressing fully, it has the quick-acting of inorganics, organic long-acting, also have multiple-effect (the dividing phosphorus decomposing potassium) property of multiple microorganism, can promote soil fertility, promote the improvement and the recovery of unbalance soil microflora, promote plant growth and improvement, improve crop quality, reduce the generation of crop disease (worm) evil, strengthen disease-resistant (worm) evil ability.
2, fermentation time generally was controlled at about 8~15 days, and leavening temperature is because the very fast intensification of reason of turning and ventilation reaches about 40~75 ℃.Can kill harmful grass seeds and intestinal bacteria.Reach the standard of the NY/T798-2004 composite microbiological fertilizer of national regulation.
Embodiment
Embodiment 1 saccharomycetic cultivation preparation
1) actication of culture: yeast starter kind is inserted the slant activation substratum activate.The weight percent of each ingredients constitute substratum is in the slant medium: glucose 1.0%, peptone 1.0%, sal epsom 0.2%, potassium primary phosphate 0.3%, dipotassium hydrogen phosphate 0.5%, agar 2.0%, add water to 100%; Adjust pH is 5.6,1.21KPa sterilization 20 minutes.
Culture environment: 28~30 ℃ of culture temperature; Incubation time 48 hours.
2) first order seed is cultivated: (is standard with 20Kg)
The activatory yeast is inoculated in the seed culture medium by 5% amount, and the seed tank culture base consists of: brown sugar (Saccharum Sinensis Roxb.) 2Kg, sal epsom 40g, potassium primary phosphate 60g, dipotassium hydrogen phosphate 100g, add water to 20Kg; Adjust pH is 5.6~7.0, boiling sterilization.
Culture environment: 28~30 ℃ of culture temperature; Incubation time 48 hours.
3) secondary seed is cultivated: (is standard with 1000Kg)
The yeast bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 10% amount, and seed culture medium is formed: brown sugar (Saccharum Sinensis Roxb.) 100Kg, sal epsom 2Kg, potassium primary phosphate 3Kg, dipotassium hydrogen phosphate 5Kg, add water to 1000Kg; Adjust pH is 5.6~7.0, boiling sterilization.
Culture environment: 28~30 ℃ of culture temperature; Incubation time 48 hours.
The cultivation preparation of embodiment 2 bacillus laterosporuses
1) actication of culture: the female access slant activation substratum of planting of bacillus laterosporus is activated.The weight percent of each ingredients constitute substratum is in the slant medium: peptone 1.0%, extractum carnis 0.3%, sodium-chlor 0.5%, agar 2.0%, add water to 100%; Adjust pH is 7.0,1.21KPa sterilization 20 minutes, and the inclined-plane is streak culture, 32~35 ℃ of culture temperature, incubation time 24 hours.
2) first order seed is cultivated: (is standard with 20Kg)
The activatory bacillus laterosporus is inoculated in the seed culture medium by 5% amount, and substratum consists of: bean powder 202g, Semen Maydis powder 200g, sodium-chlor 100g, potassium primary phosphate 100g, add water to 20Kg; Adjust pH is 7.0, boiling sterilization.
Culture environment: 32~35 ℃ of culture temperature; Incubation time 48 hours.
3) secondary seed is cultivated: (is standard with 1000Kg)
The bacillus laterosporus bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 10% amount, and substratum is formed: bean powder 10Kg, Semen Maydis powder 10Kg, sodium-chlor 5Kg, potassium primary phosphate 5Kg, add water to 1000Kg; Adjust pH is 7.0; Boiling sterilization.
Culture environment: 32~35 ℃ of culture temperature; Incubation time 72 hours.
The cultivation preparation of embodiment 3 bacillus megateriums
Cultivation preparation method with bacillus laterosporus among the embodiment 2
The production method of embodiment 4 composite microbial organic fertilizers
(1) take by weighing raw material:
500 kilograms of the castor-oil plant dregs of rice
500 kilograms of cow dungs
200 kilograms of rice husk chaffs
The bone meal double centner
800 kilograms of stalks
400 kilograms of the rape oil dregs of rice
20 kilograms of yeast bacterium liquid (accounting for the bacterium amount that connects of raw material weight 0.8%)
12.5 kilograms of bacillus laterosporus bacterium liquid (account for raw material weight 0.5% and connect the bacterium amount)
12.5 kilograms of bacillus megaterium bacterium liquid (account for raw material weight 0.5% and connect the bacterium amount)
Wherein, yeast bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 1 method obtains;
Bacillus laterosporus bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 2 methods obtain;
Bacillus megaterium bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 3 methods obtain.
(2) saccharomycetes to make fermentation: the above-mentioned castor-oil plant dregs of rice, cow dung, rice husk chaff, bone meal, straw, the rape oil dregs of rice are mixed, insert yeast (Saccharomgces cerevasae) the bacterium liquid of above-mentioned weight, after 12 hours, aerobic fermentation is carried out in ventilation, leavening temperature is controlled at 40 ℃~50 ℃, and humidity of materials is controlled at 40%~50%;
(3) bacillus laterosporus and bacillus megaterium fermentation: saccharomycetes to make fermentation 6~12 days, bacillus laterosporus (Bacillus laterosporus) the bacterium liquid and bacillus megaterium (Bacillus megaterium) the bacterium liquid that insert above-mentioned weight carried out heap fermentation 2~3 days;
(4) through the fertilizer after step (3) fermentation, add 200 kilograms urea, the vitriolate of tartar of the double superhosphate of double centner and 150 kilograms, mix, and add some conventional trace elements that use, be in harmonious proportion as iron, zinc, copper, manganese, molybdenum, boron, magnesium, promptly.
This composite microbial organic fertilizer detects, nitrogen phosphorus potassium total content 〉=20%, and water content is below 30%, viable count 〉=200,000,000/gram.Reach the standard of the NY/T 798-2004 composite microbiological fertilizer of national regulation.
The production method of embodiment 5 composite microbial organic fertilizers
(1) take by weighing raw material:
10 kilograms of the castor-oil plant dregs of rice
10 kilograms of chicken manures
10 kilograms of rice husk chaffs
10 kilograms of bone meal
10 kilograms of stalks
10 kilograms of the rape oil dregs of rice
0.3 kilogram of yeast bacterium liquid (account for raw material weight 0.5% and connect the bacterium amount)
0.06 kilogram of bacillus laterosporus bacterium liquid (account for raw material weight 0.1% and connect the bacterium amount)
0.06 kilogram of bacillus megaterium bacterium liquid (account for raw material weight 0.1% and connect the bacterium amount)
Wherein, yeast bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 1 method obtains;
Bacillus laterosporus bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 2 methods obtain;
Bacillus megaterium bacterium liquid is to cultivate the bacterium liquid of preparation according to the secondary seed that embodiment 3 methods obtain.
(2) saccharomycetes to make fermentation: the above-mentioned castor-oil plant dregs of rice, cow dung, rice husk chaff, bone meal, straw, the rape oil dregs of rice are mixed, insert yeast (Saccharomgces cerevasae) the bacterium liquid of above-mentioned weight, after 12 hours, aerobic fermentation is carried out in ventilation, leavening temperature is controlled at 40 ℃~50 ℃, and humidity of materials is controlled at 40%~50%;
(3) bacillus laterosporus and bacillus megaterium fermentation: saccharomycetes to make fermentation 6~12 days, bacillus laterosporus (Bacillus laterosporus) the bacterium liquid and bacillus megaterium (Bacillus megaterium) the bacterium liquid that insert above-mentioned weight carried out heap fermentation 2~3 days;
(4) through the fertilizer after step (3) fermentation, adds 10 kilograms urea, the vitriolate of tartar of 5 kilograms double superhosphate and 5 kilograms mixes, and adds trace elements of some conventional uses, is in harmonious proportion as iron, zinc, copper, manganese, molybdenum, boron, magnesium, promptly.
This composite microbial organic fertilizer detects, nitrogen phosphorus potassium total content 〉=20%, and water content is below 30%, viable count 〉=200,000,000/gram.Reach the standard of the NY/T 798-2004 composite microbiological fertilizer of national regulation.
Obviously, the above embodiment of the present invention only is for example of the present invention clearly is described, and is not to be qualification to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here can't give exhaustive to all embodiments.Everyly belong to the row that conspicuous variation that technical scheme of the present invention extends out or change still are in protection scope of the present invention.

Claims (6)

1. composite microbial organic fertilizer, it is characterized in that: it is to insert yeast (Saccharomgces cerevasae) the bacterium liquid that accounts for raw material weight 0.5%~0.8% by following weight proportion raw material, bacillus laterosporus (Bacillus laterosporus) the bacterium liquid that accounts for raw material weight 0.1~0.5% ferments with bacillus megaterium (Bacillus megaterium) the bacterium liquid that accounts for raw material weight 0.1~0.5%, the fermentation back adds 1~20 part urea, 0.5~10 parts double superhosphate, 0.5 the composite microbial organic fertilizer that~15 parts vitriolate of tartar is made, the water content of described composite microbial organic fertilizer are below 30%;
Wherein: described raw material weight proportioning is: 1~50 part of the castor-oil plant dregs of rice, 1~50 part of ox or chicken manure, 1~20 part of rice husk chaff, 1~10 part of bone meal, 1~80 part of straw, 1~40 part of the rape oil dregs of rice.
2. the preparation method of a composite microbial organic fertilizer as claimed in claim 1, it is characterized in that: this preparation method comprises the following steps:
(1) presses column weight amount umber proportioning and get raw material: 1~50 part of the castor-oil plant dregs of rice, 1~50 part of ox or chicken manure, 1~20 part of rice husk chaff, 1~10 part of bone meal, 1~80 part of straw, 1~40 part of the rape oil dregs of rice, 1~20 part in urea, 0.5~10 part of double superhosphate, 0.5~15 part of vitriolate of tartar;
(2) saccharomycetes to make fermentation: with 1~50 part the castor-oil plant dregs of rice, 1~50 part ox or chicken manure, 1~20 part rice husk chaff, 1~10 part bone meal, 1~80 part straw, 1~40 part the rape oil dregs of rice mix, access accounts for yeast (Saccharomgces cerevasae) the bacterium liquid of raw material weight 0.5%~0.8%, and after 12 hours, aerobic fermentation is carried out in ventilation, leavening temperature is controlled at 40 ℃~50 ℃, and humidity of materials is controlled at 40%~50%;
(3) bacillus laterosporus and bacillus megaterium fermentation: saccharomycetes to make fermentation 6~12 days, insert bacillus laterosporus (Bacillus laterosporus) bacterium liquid that accounts for raw material weight 0.1~0.5% and bacillus megaterium (Bacillus megaterium) the bacterium liquid that accounts for raw material weight 0.1~0.5% and carried out heap fermentation 2~3 days;
(4) through the fertilizer after step (3) fermentation, add 1~20 part urea, 0.5~10 part double superhosphate, 0.5~15 part vitriolate of tartar mixes, and promptly gets product, and its water content is below 30%.
3. the preparation method of composite microbial organic fertilizer according to claim 2, it is characterized in that: described saccharomycetic cultural method is: yeast starter kind being inserted the slant activation substratum activate, is to cultivate 24~48 hours under 28 ℃~30 ℃ the condition in temperature; 5%~30% the amount of activatory yeast by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The yeast bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 24~48 hours and cultivate, and promptly gets employed yeast bacterium liquid.
4. the preparation method of composite microbial organic fertilizer according to claim 2, it is characterized in that: the cultural method of described bacillus laterosporus is: the female kind of bacillus laterosporus inserted the slant activation substratum and activated, and is to cultivate 24~48 hours under 32 ℃~35 ℃ the condition in temperature; 5%~30% the amount of activatory bacillus laterosporus by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The bacillus laterosporus bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 48~72 hours and cultivate; Promptly get employed bacillus laterosporus bacterium liquid.
5. the preparation method of composite microbial organic fertilizer according to claim 2, it is characterized in that: the cultural method of described bacillus megaterium is: the female kind of bacillus megaterium inserted the slant activation substratum and activated, and is to cultivate 24~48 hours under 32 ℃~35 ℃ the condition in temperature; 5%~30% the amount of activatory bacillus megaterium by seed culture medium weight is inoculated in the seed culture medium, cultivates under the same conditions and carried out first order seed in 24~48 hours and cultivate; The bacillus megaterium bacterium liquid that first order seed is cultivated is inoculated in the secondary seed medium by 5%~30% amount of secondary seed medium weight again, cultivates under the same conditions to carry out secondary seed in 48~72 hours and cultivate; Promptly get employed bacillus megaterium bacterium liquid.
6. the preparation method of composite microbial organic fertilizer according to claim 2 is characterized in that: in step (4), interpolation trace elements iron, zinc, copper, manganese, molybdenum, boron, magnesium are in harmonious proportion.
CN2010105180036A 2010-10-25 2010-10-25 Complex microorganism organic fertilizer and preparation method thereof Pending CN102010248A (en)

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CN102584393A (en) * 2012-01-20 2012-07-18 四川农业大学 Special organic and inorganic compound fertilizer for loquat tree and preparation method for special organic and inorganic compound fertilizer
CN103613473A (en) * 2013-10-29 2014-03-05 界首市世业种植专业合作社 Dedicated organic fertilizer for bighead atractylodes rhizome plantation
CN104692921A (en) * 2015-02-03 2015-06-10 韦俊 Preparation method of special compound fertilizer for mangoes
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CN105948873A (en) * 2016-06-28 2016-09-21 贵州江口净园春茶业有限公司 Zinc and selenium-rich organic fertilizer special for tea tree and preparation method thereof
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CN106278528A (en) * 2016-07-20 2017-01-04 山东省农作物种质资源中心 A kind of Quinoa specific complex base fertilizer and preparation method thereof
CN109265217A (en) * 2018-11-12 2019-01-25 南京评宇环境工程研究院(有限合伙) A kind of production method of soil conditioner

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CN102584393A (en) * 2012-01-20 2012-07-18 四川农业大学 Special organic and inorganic compound fertilizer for loquat tree and preparation method for special organic and inorganic compound fertilizer
CN102584393B (en) * 2012-01-20 2014-07-16 四川农业大学 Special organic and inorganic compound fertilizer for loquat tree and preparation method for special organic and inorganic compound fertilizer
CN103613473A (en) * 2013-10-29 2014-03-05 界首市世业种植专业合作社 Dedicated organic fertilizer for bighead atractylodes rhizome plantation
CN104692921A (en) * 2015-02-03 2015-06-10 韦俊 Preparation method of special compound fertilizer for mangoes
CN105948873A (en) * 2016-06-28 2016-09-21 贵州江口净园春茶业有限公司 Zinc and selenium-rich organic fertilizer special for tea tree and preparation method thereof
CN105948957A (en) * 2016-07-20 2016-09-21 山东省农作物种质资源中心 Base fertilizer special for quinoa
CN105967916A (en) * 2016-07-20 2016-09-28 山东省农作物种质资源中心 Special compound fertilizer for chenopodium quinoa willd and preparation method thereof
CN106220388A (en) * 2016-07-20 2016-12-14 山东省农作物种质资源中心 A kind of Quinoa special fertilizer
CN106278528A (en) * 2016-07-20 2017-01-04 山东省农作物种质资源中心 A kind of Quinoa specific complex base fertilizer and preparation method thereof
CN109265217A (en) * 2018-11-12 2019-01-25 南京评宇环境工程研究院(有限合伙) A kind of production method of soil conditioner

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