CN101981192B - 对于热胁迫具有增强的耐受力的植物 - Google Patents

对于热胁迫具有增强的耐受力的植物 Download PDF

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CN101981192B
CN101981192B CN200880020353.0A CN200880020353A CN101981192B CN 101981192 B CN101981192 B CN 101981192B CN 200880020353 A CN200880020353 A CN 200880020353A CN 101981192 B CN101981192 B CN 101981192B
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黄亚帆
万江新
蒙妮卡·库泽马
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Abstract

本发明涉及在植物中生产一种期望的表现型的方法,通过在所述的植物中进行基因表达的操纵来完成。所述的方法涉及增加MYB‑亚群14多核苷酸或者MYB68多肽的表达水平的方式。所述的方法同样涉及一种核酸序列的表达,其中所述的核酸序列编码一个MYB‑亚群14或者MYB68转录因子。所述的方法着重于提升所述的MYB‑亚群14或者MYB68的表达水平,其中在热胁迫过程中观察到一种期望的表现型例如减少的花朵败育以及增加的产量。本发明同样涉及有效的用于这样的方法之中的核酸序列。

Description

对于热胁迫具有增强的耐受力的植物
相关申请的参考
按照35U.S.C.§119(e)的要求,所述的非临时专利申请要求享有申请日为2007年4月18日的U.S.S.N.60/925312以及申请日为2007年8月20日的U.S.S.N.60/965582的优先权。上述申请中的内容作为整体在本文中被引入作为参考。
发明领域
本发明属于植物分子生物学领域并且涉及具有新的表现型的转基因植物,生产这样的植物的方法以及有效的用于这样的方法之中的多核苷酸以及多肽。更具体的,本发明涉及所述的MYB多核苷酸的使用以及表达这种多核苷酸以及多肽的转基因植物。
背景技术
环境的胁迫是形成农业作物的显著的产量降低的原因。除了先前公开的许多报道之外,在最近的几年中对于整个美国在1982-1998年的时间里气候的变化与玉米以及大豆的产量之间的关系进行了研究(参见Lobell以及Asner于2003年发表的文章)。逐步的温度变化已经对农作物的产量产生了可以测量的影响。已经估算出,当所述的生长温度每超出季节最适宜温度上升一度,玉米以及大豆的产量降低17%。在1990年至2010年间,随着预测温度的增长为1.4℃至5.8℃(联合国政府间气候变化专门委员会(IPCC)工作组I,2001年),农作物植物对于高温的耐受性的改进已经成为农业生物技术发展的主要焦点中的一个。
单子叶植物以及双子叶植物在开花以及结实的形成过程中对热胁迫均格外敏感,并且因此热胁迫对于种子的产量具有显著的影响(参见Young等人于2004年发表的文章;Sato等人于2002年发表的文章;Angadi等人于2000年发表的文章;Carlson于1990年发表的文章;Wahid A,Gelani S,Ashraf M以及Foolad M.R.于2007年发表的文章)。已经提出植物拥有先天的基础的热耐受性能力以及后天获得的热耐受性,并且在不同的植物品种中存在着共同的热应答(参见Kapoor等人于1990年发表的文章;Vierling于1991年发表的文章;Flahaut等人于1996年发表的文章;Burke等人于2000年发表的文章;Hong以及Vieling于2000年发表的文章;Massie等人于2003年发表的文章;Larkindale等人于2005年发表的文章)。基础的热耐受性允许植物在被暴露于高于最适的生长温度时能够存活,而后天获得的热耐受性是由在一种亚致死的热胁迫条件下进行的短暂的驯化周期所引发的,这使得植物能够在随后的热胁迫条件下存活下来,然而所述的热胁迫条件应当是致命的。为了对在植物的热耐受性中所涉及到的基因以及途径进行识别以及定义,已经进行了大量的研究。例如,热休克转录因子(HSF)以及热休克蛋白(HSP)已经受到了大量的关注,用来阐明这些基因在应答于热胁迫时所发挥的作用以及所产生的功效,从而像存在于植物生长激素例如脱落酸以及乙烯一样。
转录因子是一种DNA结合蛋白,能够与特异性的启动子序列或者增强子序列发生相互作用并且改变所述的相关基因的基因表达。当与所述的转录因子发生结合的所述的特异性序列与一组基因相关联时,整体的途径能够同等的受到各种不同的组分基因的调节,同时发生上调节或者去调节。转录因子可以同等的改变一组基因,从而对一种刺激进行应答,其中所述的刺激是例如一种环境胁迫,营养状况或者病原体侵袭,或者可以是一种信号途径的一个组成部分,其中所述的信号途径是例如一种激素信号途径。转录因子拥有一种模块结构并且主要依据DNA结合结构域为基础被进行了分类。
转录因子的MYB家族由至少198个基因构成(参见Yanhui等人于2006年发表的文章)并且其已经被提议在各种不同的过程中具有调节功能,所述的过程涉及从生长到形成防御应答的范围。植物MYB蛋白根据不完全的MYB重复的存在以及数量进行了分类,其中所述的每个不完全的MYB重复(MYB repeats)由大约52个氨基酸构成。所述的MYB结构域形成了一种螺旋-转角-螺旋构象并且代表着所述的DNA结合结构域。MYB蛋白的三种主要的群体被分为R1R2R3-MYB,R2R3-MYB以及MYB-相关性蛋白。
拟南芥属的R2R3-MYB蛋白家族由125个蛋白组成并且其特征在于在它们的N-末端具有一个R2R3 DNA结合结构域(参见Kranz等人于1998年发表的文章,以及Stracke等人于2001年发表的文章)。这些基因在各种生物学过程中都有所涉及,所述的生物学过程包括激素作用的调节,次级代谢(参见Paz-Ares等人于1987年发表的文章),细胞的形态形成的控制(参见Oppenheimer等人于1991年),分生组织、花以及种子的形成(参见Kirik等人于1998年发表的文章,Schmitz等人于2002年发表的文章)以及对于各种环境因素的应答(参见Kranz等人于1998年发表的文章;Jin以及Martin于1999年发表的文章;Meissner等人于1999年发表的文章)。
MYB序列已经被进一步划分成各种亚群,所述的划分是以序列为依据的(参见Krantz等人于1998年发表的文章,Stracke等人于2001年发表的文章)。Krantz等人于1998年识别出MYB68落入亚群14的范围之内,而MYB36以及MYB84同样如此。然而,Stracke等人于2001年指出在亚群14中额外包括所述的MYB37,MYB38以及MYB87。Stracke进一步记录了基因的功能保守性的几种情况,其中所述的基因在所述的系统树形图中是集群在一起的。
所述的R2R3-MYB家族的分类已经在拟南芥(At)中识别出125种MYB蛋白。R2R3 MYB基因的特征在于MYB结构域,所述的结构域含有两个53个氨基酸(aa)的不完全重复(R2,以及R3)。每个重复(repeat)含有三个螺旋-转角-螺旋结构。所述的R2结构域以及R3结构域位于所述蛋白的N-末端的附近。每个重复之上的最后两个螺旋与它们之间的环一同形成了一种DNA-结合基序结构,这与螺旋-环-螺旋(HLH)蛋白相似。所述的第三个螺旋直接与DNA进行结合,而所述的第一个螺旋以及第二个螺旋为形成所述的螺旋-环-螺旋基序的构象作出了贡献,其中所述的螺旋-环-螺旋基序对于特异性基因靶向的识别而言似乎是重要的(参见Ogata等人于1994年发表的文章;William以及Grotewold于1997年发表的文章;Jia等人于2004年发表的文章)。所述的R2R3-MYB蛋白进一步被定义为22种亚群,这是依照它们的动植物种类间的关系,以除所述的MYB结构域之外的至少一个共享的氨基酸基序为基础来定义的(参见Kranz等人于1998年发表的文章)。AtMYB68,AtMYB84,以及AtMYB36被归类为亚群14之中,这是以存在于所述蛋白的所述C-末端处的两个共享基序为依据的,其中所述的两个共享基序是:S1:SFSQLLLDPN序列识别号(SEQ ID NO):266以及S2:TSTSADQSTISWEDISEQID NO:267。这些基序的同源性是有限的,例如,AtMYB36仅仅具有20%的同一性。其次,AtMYB87,AtMYB37以及AtMYB38同样被包括在亚群14之中,这是以所述的MYB DNA结构域中的序列保守性:R2以及R3的螺旋-转角-螺旋重复为依据的(参见Stracke等人于2001年发表的文章)。
所述的R2R3结构域可以作为对于特异性的DNA结合的指示,其中所述的结合是经由所述的R3结构域中的所述第三个螺旋的独特的氨基酸序列来进行的,并且次要的构象变化与存在于所述的前两个螺旋的结构间相互作用发生关联。已经提出亚群14的成员可能是功能上多余的直向同源基因。例如,已经在拟南芥的侧生分生组织萌发中进行了针对MYB-亚群14的研究(参见Muller等人于2006年发表的文章)。MYB-亚群14中的所有成员表现出了与所述的番茄Blind(B1)基因的高度相似性,其中所述的番茄Blind基因是一种腋生分生组织调节剂。利用逆转录-聚合酶链式反应(RT-PCR)在组织中探测到四种成员的转录产物:AtMYD37,AtMYB38,AtMYB84以及AtMYB87,其中所述的组织包括茎尖,节间,叶片,花蕾,开放的花朵,以及根,而AtMYB36以及AtMYB68的表达在根组织中进行了表达。利用AtMYB37,AtMYB38以及AtMYB84的敲除来进行的表现型的分析表明,对于调节腋芽的形成而言,MYB-亚群14中的这些成员至少是部分多余的。
MYB68是一种R2R3类型的MYB基因,并且属于MYB-亚群14中的一个成员,其已经在一项转座子基因捕获研究中被识别出来(参见Feng等人于2004年发表的文章)。已经证明了这种基因的表达对于根部的中柱鞘细胞具有特异性。在所述的无价值的突变体中,没有可以探测到的MYB68mRNA(信使核糖核酸);然而,当植物在标准的环境下进行生长时,没有显示出突变的表现型。在对于存在于各种不同的生长环境下的MYB68进行的评价中,仅仅当植物在热的温室条件下(30-40℃)进行生长时,才辨认出唯一的一种表现型,所述的表现型是植物的叶片面积的减少。通过使用一种野生型MYB68基因对所述的myb68突变体进行后台转化,从而对这种表现型进行了援救。对于在发根培养器中生长的所述的myb68突变体的根部组织进行的检查表明,具有增加的生物量水平以及木质素水平。笔者推定在根部的形成过程中涉及到MYB68(参见Feng等人于2004年发表的文章)。
转录的激活作用首先经由转录因子进行介导,其中所述的转录因子与增强子元件以及启动子元件发生相互作用。转录因子与这样的DNA元件所进行的结合构成了转录开始的一个至关重要的步骤。每个转录因子同存在于启动子中的与所述的转录因子具有特异性的结合序列进行结合,并且通过与辅激活因子和/或蛋白间发生的相互作用激活所述的连接的编码区域的表达,其中所述的辅激活因子和/或蛋白属于所述的转录复合体中的一部分。
发明内容
本发明涉及一种增加植物的热胁迫耐受性的方法,其中所述的方法通过增加MYB亚群-14多肽的表达的方式来完成。增加的热胁迫耐受性包括在热胁迫的条件下以及经过热胁迫的条件之后具有改善的结实现象。改善的结实现象导致了增加的产量。MYB-亚群-14多肽包括例如MYB68多肽,MYB36多肽,MYB84多肽,MYB37多肽,MYB38多肽或者MYB87多肽。优选的,所述的MYB-亚群-14多肽是MYB68多肽,MYB36多肽或者MYB84多肽。所述的MYB-亚群-14多肽的表达是异位表达,或者是组成性表达。可以选择的,所述的MYB亚群-14多肽的表达发生在其典型的表达位点上,例如,根部组织。
一种热胁迫耐受性的植物是通过下述方法制备得到的:提供一种核酸构建体,其中所述的核酸构建体能够增强所述的Myb亚群-14多肽的表达,将所述的核酸构建体插入到一种载体之中,利用所述的载体对一种植物、组织培养物、或者植物细胞进行转化,从而获得一种具有增加的Myb亚群-14多肽的表达的植物、组织培养物或者植物细胞,并且使所述的植物进行生长或者从所述的组织培养物或者植物细胞中进行一种植物的再生。一种能够增强所述的Myb亚群-14多肽的表达的核酸构建体包括例如一种增强子元件。增强子是一种在真核细胞以及某些真核细胞病毒中发现的序列,当其位于与所述的相关基因距离高达几千碱基对的任意方位上时,它能够增强所述基因的转录。当位于上述谈论的基因的5’侧(上游)上时,这些序列发挥了增强子的作用。然而,当被放置于所述基因的3’侧(下游)上时,某些增强子是具有活性的。所述的增强子元件能够激活基因的转录并且改变所述的内源性基因的正常的表达类型。增强子元件对于本领域技术人员而言是已知的。例如所述的增强子元件是一种35S增强子元件。
除此之外,一种能够增强所述的Myb亚群-14多肽的表达的核酸构建体包括例如一种编码Myb亚群-14多肽的核酸。范例性的MYB多肽以及核酸包括那些序列识别号(SEQ IDNO)为1-265的多肽以及核酸。所述的编码Myb亚群-14多肽的核酸与一种启动子发生了可操作性的连接。所述的启动子是一种异源启动子或者是一种同源启动子。除此之外,所述的启动子是一种组成型启动子或者是一种诱导型启动子。
通过增加所述的MYB亚群-14多肽的表达,意味着由所述的经过所述核酸构建体进行转化的细胞所生产的量高于没有经过所述的核酸构建体进行转化的细胞所生产的量,其中所述的没有进行转化的细胞是例如一种对照细胞。对照细胞包括例如一种能够内源性的表达MYB亚群-14多肽的细胞,例如一种植物根细胞,或者对照细胞是与所述的转化细胞具有相同的细胞类型的一种非转化细胞,是一种叶片细胞、分生组织细胞或者花朵细胞或者种子细胞。增加指的是一倍的增加,两倍的增加,三倍的增加或者更高倍数的增加。表达上的增加同样意在包括在一种通常情况下不产生MYB亚群-14多肽的细胞中进行的MYB亚群-14多肽的表达。
同样被包括在本发明之内的是一种识别热胁迫耐受性植物的方法。与一种对照植物相比,通过这些方法识别出的所述植物具有减少的花朵败育以及增加的产量。热胁迫耐受性植物是通过下述方式被识别出来的:将一群开花植物暴露于热胁迫处理中并且从上述的植物群体中选择一种具有减少的花朵败育的植物。热胁迫处理包括例如将所述的植物在足够热的温度下暴露足够长的时间,这样一来导致了对植物的功能或者发育上的损伤。减少的花朵败育意味着植物不会损失与因花朵败育而损失的同样多的花朵,或者与另外一种被暴露于类似的热胁迫水平下的植物相比,所述的植物具有更高的结实产量。与一种对照植物相比,具有减少的花朵败育的植物在结实产量上具有5%,10%,20%,25%,30%或者更高的增长。
本发明进一步包括由本发明中所述的方法制备得到的所述植物,以及由所述的植物所生产的种子,其中所述的种子能够生产出对热胁迫具有增强的耐受力的植物。
除非另外定义,这里所使用的全部的技术术语以及科学术语具有与本发明所属技术领域的普通技术人员通常所理解的含义相同的含义。尽管在本发明的实践或者检验过程中可能使用到那些与这里所描述的方法以及材料相类似或者等价的方法以及材料,适宜的方法以及材料在下文中进行了描述。这里所提及的全部出版物、专利申请、专利、以及其他参考文献均作为整体被引入作为参考。如果存在矛盾的情形,所述的说明书,包括定义,将起到主导作用。除此之外,所述的材料、方法、以及实施例仅仅是描述性的并且并不意在构成限制。
本发明的其他特征以及优点将通过下面的详细描述以及所述的权利要求而变得显而易见。
具体实施方式
本发明是以对于下述植物的令人惊奇的发现为基础的:与一种野生型的对照组相比,所述的植物对热胁迫具有增强的耐受性,这种耐受性导致了增加的产量。更具体的,本发明是以下述发现为基础的:增加所述的MYB-亚群14多肽(例如,MYB68)的表达导致了一种植物的产生,所述的植物对热胁迫具有增强的抗性。
MYB-亚群14多肽的表达可以通过例如下述方式来完成:通过增加所述的内源性MYB-亚群14多肽的表达(例如,激活标签的插入)的方式或者通过编码MYB-亚群14多肽的外源性基因构建体的表达的方式。所述的用于编码所述MYB-亚群14多肽的基因对于被转化的物种而言可以是内源性的或者是外源性的。正如实施例中所表示的,对热胁迫具有增强的抗性的植物不仅仅是通过利用其自身的MYB-亚群14多肽对植物进行转化而制备得到的,而且也包括利用来自于另外一个植物物种的MYB-亚群14多肽对植物进行转化。
因此本发明提供了用以增强(例如,增加)植物的热胁迫耐受性的方法,其中所述的方法通过增加所述的MYB亚群-14多肽的表达来实现。同样被包括在本发明之内的是一种识别热胁迫耐受性植物的方法。与一种对照植物相比,通过这些方法识别出的所述植物具有减少的花朵败育以及增加的产量。热胁迫耐受性的植物是通过下述方式被识别出来的:将一群开花植物暴露于热胁迫处理中并且从上述的植物群体中选择一种具有减少的花朵败育的植物。本发明同样包括由本发明中所述的方法制备得到的转基因植物,以及由所述的转基因植物所生产的种子,其中所述的种子能够生产出一种对热胁迫具有耐受性的植物。
除非另外定义,这里所使用的全部的技术术语以及科学术语具有与本发明所属技术领域的普通技术人员通常所理解的含义相同的含义。尽管在本发明的实践或者检验过程中可能使用到那些与这里所描述的方法以及材料相类似或者等价的方法以及材料,适宜的方法以及材料在下文中进行了描述。这里所提及的全部出版物、专利申请、专利、以及其他参考文献均作为整体被引入作为参考。如果存在矛盾的情形,所述的说明书,包括定义,将起到主导作用。除此之外,所述的材料、方法、以及实施例仅仅是描述性的并且并不构成限制。
出于方便的目的,在对本发明进行进一步的描述之前,在这里对所述的说明书、实施例及后附的权利要求中使用到的某些术语进行定义。这些定义应当依照所述的公开内容的其余部分以及按照所属领域普通技术人员的理解来进行阅读。
所述的术语“组成性表达”指的是基因通过一种不规则的方式以恒定的水平在任意的细胞中所进行的表达。
所述的术语“cMYB”以及“MYB”指的是MYB的cDNA克隆,并且两者是可以互换使用的。在使用到或者提及到基因组序列的情况下,利用所述的术语“gMYB”或者“基因组MYB”对其进行识别以及区分,从而指的是基因组MYB序列。
所述的术语“异位表达”指的是相对于所述的内源性基因表达而言,基因在有机体的异常位置处所进行的表达。异位表达可能包括进行了组成性表达的基因,这取决于所述的给定基因的天然表达类型。
所述的术语“表达盒”指的是一种载体构建体,基因在所述的载体构建体中进行转录。除此之外,所述的经表达的mRNA(信使核糖核酸)可以被翻译成一种多肽。
所述的术语“表达”或者“过表达”可以互换使用并且指的是能够进行转基因的表达的一种基因的表达形式。可以使一个细胞中的全部表达水平相对于野生型细胞而言发生提升。
“花朵败育”指的是不能发育并且生产出果实或者种子的花朵。除了花朵的早熟性衰老之外,花朵败育可以指的是花粉产量的损失,授粉以及授精的改变以及随后的种子发育。分生组织的生长以及发育的改变,特别是花朵分生组织的生长以及发育的改变,也进一步的被包括在所述的花朵败育的含义之内。
所述的术语“热耐受性”被定义为一种表现型,在所述的表现型中,一种植物或者植物系具有增强的能力,能够抵挡住升高的温度,并且与另外一种植物或者植物系相比较,其生产的产量超过所述的另外一种植物或者植物系,其中所述的另外一种植物系是一种对照植物,例如是一种野生型对照植物系。
“启动子序列”,或者“启动子”,指的是一种核酸序列,所述的核酸序列能够在一种植物细胞中诱导一种可操作性连接的基因序列进行转录。
所述的术语“结实”是种子的形成,所述的种子的形成是花朵的授粉以及随后的卵细胞的授精以及受精卵的发育所产生的结果。结实的减少将对所生产的种子数量形成联网式的减少(net reduction),其中所述的结实的减少的发生可能归因于上述任意一个步骤的中断。
所述的术语“本质上相类似”指的是当核酸中的一个或者多个核苷发生变化时,并不会对所述的核酸或者其编码的多肽的功能性质产生改变。由于所述的遗传密码的退化,一个碱基对的改变可能不会在所述被编码的氨基酸序列中产生变化。例如,所述的密码子ACT,ACC,ACA以及ACG全部编码一种苏氨酸氨基酸。或者,一个或者多个碱基对的改变可能使所述被编码的氨基酸产生变化,然而如果所述的替代氨基酸具有相类似的化学性质,那么所述被编码的蛋白的功能性可能不会受到影响。例如,当苏氨酸密码子ACT以及ACC分别变化为AGT或者AGC时,其编码丝氨酸,一种化学性质以及生物性质相类似的氨基酸。除此之外,在多肽中的某些氨基酸是非必需的,因而可以在这些位置处进行改变,而不会对所述多肽的功能性产生影响。本质上相类似同样指的是在一个或者多个核苷碱基上存在变化的序列,其中所述的变化不会对所述序列改变基因表达的能力产生影响,这是通过各种基因沉默方法来完成的,所述的基因沉默方法是例如反义RNAi或者共抑制。所述的术语“本质上相类似”指的是在一个或者多个氨基酸上存在变化的多肽,如上所述,其中所述的变化不会对所述多肽的功能性质产生改变。
所述的术语“产量”指的是种子的数量,种子的重量,种子的大小,全部植物的生物量,增加的植物器官的生物量,果实的产量,以及花朵的产量,其中所述的植物器官是例如茎或者叶片或者根。
所述的术语“产量保护”被定义为当经过了强加的胁迫之后,存在于所述的转基因或者突变体的产量与所述的对照产量之间的正向差异,用%值来表示,其中所述的产量被表示成最佳产量的%。通过对所述的最佳产量与经过了所述的胁迫处理之后的产量进行比较来完成所述的计算(胁迫产量/最佳产量x 100)。
根据序列的同源性以及上述定义的结构域以及基序的存在将所述的MYB基因家族进行分类,其中上述定义的结构域是例如R2R3结构域。所述的分类并不是在所有的情况下都是绝对的,可以根据进行分析所选择的标准发生改变(参见Krantz等人于1998年发表的文章,Stracke等人于2001年发表的文章)。
在这里我们所定义的所述MYB-亚群14包括至少下述的成员,MYB68,MYB36,MYB37,MYB38以及MYB87。依照这里所描述的方法以及表格1中所涵盖的实施例,利用所述的AtMYB68,MYB36,MYB84,MYB37,MYB38以及MYB87序列从其他的物种中对同系物进行识别。
所述的术语“MYB序列”指的是一种在前后文中适宜的多核苷酸序列或者多肽序列。
序列
下述是来自于所述的MYB-亚群14家族中的序列,以及相应的序列标识符,这些序列以及序列标识符是在所述的说明书、实施例以及后附的权利要求中使用到的序列以及标识符:
表1
序列号: 物种 登录号对照 MYB识别
1 拟南芥 NM_125976.2 MYB68 NT
2 拟南芥 NP_201380.1 MYB68 AA
3 拟南芥 NM_114829.3 MYB84 NT
4 拟南芥 NP_190538.1 MYB84 AA
5 拟南芥 NM_125143.3 MYB36 NT
6 拟南芥 NP_200570.1 MYB36 AA
7 芜菁 MYB68 NT
8 芜菁 MYB68 AA
9 水稻 NM_001057941.1 MYB36 NT
10 水稻 AAT85046.1 MYB36 AA
11 棉花 TC34239 MYB68 NT
12 棉花 TC34239_ORF MYB68 AA
13 大豆 DQ822965.1 MYB84 NT
14 大豆 ABH02906.1 MYB84 AA
15 大豆 MYB84 NT
16 大豆 MYB84 AA
17 玉米 TC370133 MYB84 NT
18 玉米 TC370133_ORF MYB84 AA
19 高粱 AF474127 MYB36 NT
20 高粱 AAL84760.1 MYB36 AA
21 小麦 BQ483726 MYB84 NT
22 小麦 BQ483726_ORF MYB84 AA
23 杨属 TC54478 MYB84 NT
24 杨属 TC54478_ORF MYB84 AA
25 截形苜蓿 TC97441 MYB68 NT
26 截形苜蓿 TC97441_ORF MYB68 AA
27 番茄 AF426174.1 MYB36 NT
28 番茄 AAL69334.1 MYB36 AA
29 番茄 BG134669 MYB36 NT
30 番茄 BG134669_ORF MYB36 AA
31 拟南芥 NM_119940.3 MYB87 NT
32 拟南芥 NP195492.2 MYB87 AA
33 拟南芥 NM_122206.3 MYB37 NT
34 拟南芥 NP_197691.1 MYB37 AA
35 拟南芥 NM_129245.2 MYB38 NT
36 拟南芥 NP_181226.1 MYB38 AA
37 拟斯贝尔托山羊草 BQ841600.1 MYB36 NT
38 金鱼草 AJ794728.1 MYB68 NT
39 金鱼草 AJ794728.1_ORF MYB68 AA
40 耧斗菜 TC13008 MYB84 NT
41 耧斗菜 TC13008_ORF MYB84 AA
42 耧斗菜 TC11167 MYB36 NT
43 耧斗菜 TC11167_ORF MYB36 AA
44 花生 CD038321.1 MYB68 NT
45 花生 CD038321.1_ORF MYB68 AA
46 花生 ES761155.1 MYB68 NT
47 花生 ES761155.1_ORF MYB68 AA
48 花生属野生种 EH046152.1 MYB36 NT
49 花生属野生种 EH046152.1_ORF MYB36 AA
50 二穗短柄草 DV486330.1 MYB38 NT
51 二穗短柄草 DV486330.1_ORF MYB38 AA
52 二穗短柄草 DV488965.1 MYB38 NT
53 二穗短柄草 DV488965.1_ORF MYB38 AA
54 油菜(蓓蕾) MYB68 NT
55 油菜(蓓蕾) MYB68 AA
56 油菜(根) MYB68 NT
57 油菜(根) MYB68 AA
58 油菜 TC40384 MYB68 NT
59 油菜 ES900275.1 MYB68 NT
60 油菜 ES900275.1_ORF MYB68 AA
61 油菜 TC55899 MYB38 NT
62 油菜 TC55899_ORF MYB38 AA
63 芜菁 EX134980.1 MYB68 NT
64 芜菁 EX134980.1_ORF MYB68 AA
65 芜菁 EX137439.1 MYB68 NT
66 芜菁 EX137439.1_ORF MYB68 AA
67 红花 EL384492.1 MYB36 NT
68 红花 EL384492.1_ORF MYB36 AA
69 红花 EL392277.1 MYB36 NT
70 红花 EL392277.1_ORF MYB36 AA
71 斑点矢车菊 EH724496.1 MYB36 NT
72 斑点矢车菊 EH724496.1_ORF MYB36 AA
73 斑点矢车菊 EH719165.1 MYB36 NT
74 斑点矢车菊 EH719165.1_ORF MYB36 AA
75 斑点矢车菊 EH72443.8.1 MYB68 NT
76 斑点矢车菊 EH724438.1_ORF MYB68 AA
77 黄色矢车菊 EH774519.1 MYB68 NT
78 黄色矢车菊 EH74519.1_ORF MYB68 AA
79 黄色矢车菊 EH771972.1 MYB68 NT
80 黄色矢车菊 EH771972.1_ORF MYB68 AA
81 黄色矢车菊 EH768792.1 MYB84 NT
82 黄色矢车菊 EH768792.1_ORF MYB84 AA
83 栽培菊苣 EL361859.1 MYB84 NT
84 栽培菊苣 EL361859.1_ORF MYB84 AA
85 欧洲菊苣 EH681135.1 MYB38 NT
86 欧洲菊苣 EH681135.1_ORF MYB38 AA
87 欧洲菊苣 EH694860.1 MYB68 NT
88 甜橙 CK936024.1 MYB68 NT
89 甜橙 CK936024.1_ORF MYB68 AA
90 中粒咖啡 DV692261.1 MYB37 NT
91 中粒咖啡 DV691112.1 MYB37 NT
92 厚皮甜瓜 AM727197.2 MYB36 NT
93 厚皮甜瓜 AM727197.2_ORF MYB36 AA
94 厚皮甜瓜 AM716075.2 MYB36 NT
95 厚皮甜瓜 AM716075.2_ORF MYB36 AA
96 野胡萝卜 AB298508.1 MYB68 NT
97 野胡萝卜 BAF49444.1 MYB68 AA
98 非洲油棕榈 EL690464.1 MYB84 NT
99 非洲油棕榈 EL690464.1_ORF MYB84 AA
100 美洲油棕榈 ES370938.1 MYB84 NT
101 美洲油棕榈 ES370938.1_ORF MYB84 AA
102 花菱草 CD480801.1 MYB68 NT
103 花菱草 CD480801.1_ORF MYB68 NT
104 乳浆大戟 DV138530.1 MYB84 NT
105 乳浆大戟 DV138530.1_ORF MYB84 AA
106 乳浆大戟 DV126436.1 MYB36 NT
107 乳浆大戟 DV126436.1_ORF MYB36 AA
108 绿玉树 BP958179.1 MYB84 NT
109 银杏叶 EX940876.1 MYB68 AA
110 大豆 MYB84 NT
111 大豆 MYB84 AA
112 大豆 TC213651 MYB84 NT
113 大豆 TC213651_ORF MYB84 AA
114 大豆 DQ822971.1 MYB36 NT
115 大豆 ABH02912.1 MYB36 AA
116 大豆 TC211227 MYB36 NT
117 大豆 TC211227_ORF MYB36 AA
118 棉花 TC62721 MYB68 NT
119 棉花 TC62721_ORF MYB68 AA
120 棉花 DW491290.1 MYB36 NT
121 棉花 DW491290.1_ORF MYB36 AA
122 剑叶耳草全草 CB077617.1 MYB84 NT
123 剑叶耳草全草 CB077617.1_ORF MYB84 AA
124 向日葵 BQ96758 MYB36 NT
125 绢毛葵 EE621630.1 MYB36 NT
126 绢毛葵 EE621630.1_ORF MYB36 AA
127 绢毛葵 EE619500.1 MYB36 NT
128 绢毛葵 EE619500.1_ORF MYB36 AA
129 蓝茎向日葵 EL422629.1 MYB68 NT
130 蓝茎向日葵 EE645503.1 MYB68 NT
131 蓝茎向日葵 EE645503.1_ORF MYB68 AA
132 蓝茎向日葵 EE646813.1 MYB36 NT
133 蓝茎向日葵 EE646813.1_ORF MYB36 AA
134 HELIANTHUSPARADOXUS EL474327.1 MYB84 NT
135 美洲野生向日葵 DY942970.1 MYB84 NT
136 美洲野生向日葵 DY942970.1_ORF MYB84 AA
137 美洲野生向日葵 DY953493.1 MYB68 NT
138 美洲野生向日葵 DY953493.1_ORF MYB68 AA
139 菊芋 EL445341.1 MYB36 NT
140 菊芋 EL445341.1_ORF MYB36 AA
141 大麦 BY845215.1 MYB38 NT
142 大麦 BY845215.1_ORF MYB38 AA
143 啤酒花 AJ876882 MYB36 NT
144 啤酒花 CAI46244.1 MYB36 AA
145 宿根莴苣 DW092247.1 MYB84 NT
146 宿根莴苣 DW092247.1_ORF MYB84 AA
147 宿根莴苣 DW065247.1 MYB68 NT
148 宿根莴苣 DW065247.1_ORF MYB68 AA
149 莴笋 DY960463.1 MYB38 NT
150 莴笋 DY960463.1_ORF MYB38 AA
151 莴笋 DY969483.1 MYB38 NT
152 莴笋 DY969483.1_ORF MYB38 AA
153 莴笋 DY980672.1 MYB38 NT
154 莴笋 DY980672.1_ORF MYB38 AA
155 野生祖先毒莴笋 DW108054.1 MYB38 NT
156 毒莴笋 DW160139.1 MYB84 NT
157 毒莴笋 DW160139.1_ORF MYB84 AA
158 毒莴笋 DW160891.1 MYB38 NT
159 毒莴笋 DW160891.1_ORF MYB38 AA
160 北美鹅掌揪 CO998829.1 MYB38 NT
161 北美鹅掌揪 CO998829.1_ORF MYB38 AA
162 栽培苹果 DT002401.1 MYB36 NT
163 栽培苹果 DT002401.1_ORF MYB36 AA
164 栽培苹果 DQ074472.1 MYB38 NT
165 栽培苹果 AAZ20440.1 MYB38 AA
166 木薯 DB936694.1 MYB68 NT
167 木薯 DB936694.1_ORF MYB68 AA
168 地钱 BJ846153.1 MYB84 NT
169 地钱 BJ846153.1_ORF MYB84 AA
170 截形苜蓿 TC110497 MYB36 NT
171 截形苜蓿 TC110497_ORF MYB36 AA
172 截形苜蓿 BF634640 MYB84 NT
173 截形苜蓿 BF634640_ORF MYB84 AA
174 欧亚萍蓬草 CD472544.1 MYB36 NT
175 欧亚萍蓬草 CD472544.1_ORF MYB36 AA
176 水稻 LOC_Os01g09590.1_cds MYB38 NT
177 水稻 LOC_Os01g09590.1 MYB38 AA
178 水稻 LOC_Os01g49160.1_cds MYB36 NT
179 水稻 LOC_Os01g49160.1 MYB36 AA
180 水稻 LOC_Os01g52410.1_cds MYB38 NT
181 水稻 LOC_Os01g52410.1 MYB38 AA
182 水稻 LOC_Os02g54520.1_cds MYB36 NT
183 水稻 LOC_Os02g54520.1 MYB36 AA
184 水稻 LOC_Os05g48010.1_cds MYB36 NT
185 水稻 LOC_Os05g48010.1 MYB36 AA
186 水稻 LOC_Os08g15020.1_cds MYB36 NT
187 水稻 LOC_Os08g15020.1 MYB36 AA
188 水稻 LOC_Os09g26170.1_cds MYB36 NT
189 水稻 LOC_Os09g26170.1 MYB36 AA
190 水稻 LOC_Os10g35660.1_cds MYB36 NT
191 水稻 LOC_Os10g35660.1 MYB36 AA
192 云杉 EX361512.1 MYB68 NT
193 云杉 EX361512.1_ORF MYB68 AA
194 云杉 TC20498 MYB68 NT
195 云杉 TC20498_ORF MYB68 AA
196 松属 DR015810 MYB84 NT
197 松属 DR015810_ORF MYB84 AA
198 松属 TC66643 MYB68 NT
199 松属 TC66643_ORF MYB68 AA
200 枸橘 CD575120.1 MYB38 NT
201 枸橘 CD575120.1_ORF MYB38 AA
202 杨属 Gw1.II.96.1 MYB68 NT
203 杨属 Gw1.II.96.1_ORF MYB68 AA
204 杨属 DB879439.1 MYB84 NT
205 杨属 DB879439.1_ORF MYB84 AA
206 杨属 TC74579 MYB36 NT
207 杨属 TC74579_ORF MYB36 AA
208 无梗花栎 CU639795.1 MYB36 NT
209 无梗花栎 CU639795.1_ORF MYB36 AA
210 西班牙栓皮栎 EE743680.1 MYB84 NT
211 野萝卜 FD544184.1 MYB68 NT
212 野萝卜 FD544184.1_ORF MYB68 AA
213 野萝卜 EY915531.1 MYB68 NT
214 野萝卜 FD540311.1 MYB68 NT
215 野萝卜 FD540311.1_ORF MYB68 AA
216 野萝卜 EV548164.1 MYB38 NT
217 萝卜 FD580369.1 MYB68 NT
218 杂交蔷薇 EC587279.1 MYB68 NT
219 杂交蔷薇 EC587279.1_ORF MYB68 AA
220 甘蔗 CA150911 MYB36 NT
221 甘蔗 CA150911_ORF MYB36 AA
222 甘蔗 CA258665 MYB84 NT
223 甘蔗 CA258665_ORF MYB84 AA
224 甘蔗 TC44677 MYB36 NT
225 甘蔗 TC44677_ORF MYB36 AA
226 黑麦 BE495537 MYB38 NT
227 黑麦 BE495537_ORF MYB38 AA
228 番茄 TC182203 MYB36 NT
229 番茄 TC182203_ORF MYB36 AA
230 马铃薯 AM907873.1 MYB36 NT
231 马铃薯 AM907873.1_ORF MYB36 AA
232 高粱 TC98185 MYB36 NT
233 高粱 TC98185_ORF MYB36 AA
234 高粱 TC101637 MYB36 NT
235 高粱 TC101637_ORF MYB36 AA
236 拟高粱 BG560270.1 MYB36 NT
237 拟高粱 BG560270.1_ORF MYB36 AA
238 欧蒲公英 DY830100.1 MYB68 NT
239 欧蒲公英 DY830100.1_ORF MYB68 AA
240 TRIPHYSARIAPUSILLA EY172046.1 MYB68 NT
241 TRIPHYSARIAPUSILLA EY172046.1_ORF MYB68 AA
242 TRIPHYSARIAPUSILLA EY179359.1 MYB36 NT
243 TRIPHYSARIAPUSILLA EY179359.1_ORF MYB36 AA
244 TRIPHYSARIAPUSILLA EY174724.1 MYB38 NT
245 TRIPHYSARIAPUSILLA EY174724.1_ORF MYB38 AA
246 直果草属 EX989121.1 MYB38 NT
247 直果草属 EY018825.1 MYB38 NT
248 直果草属 EY018825.1_ORF MYB38 AA
249 小麦 MYB84 NT
250 小麦 MYB84 AA
251 湿地越橘 CV090776.1 MYB36 NT
252 湿地越橘 CV090776.1_ORF MYB36 AA
253 葡萄 CAO70108.1_cds MYB84 NT
254 葡萄 CAO70108.1 MYB84 AA
255 葡萄 CAO43296.1_cds MYB36 NT
256 葡萄 CAO43296.1 MYB36 AA
257 葡萄 CAO61524.1_cds MYB84 NT
258 葡萄 CAO61524.1 MYB84 AA
259 葡萄 DT006424 MYB36 NT
260 葡萄 DT006424-ORF MYB36 AA
261 玉米 MYB36 NT
262 玉米 MYB36 AA
263 玉米 TC320820 MYB36 NT
264 玉米 TC320820_ORF MYB36 AA
265 水稻 MYB36 NT
确定两个序列或者多个序列之间的同源性
为了确定两个氨基酸序列或者两个核酸的同源性百分数,对所述的序列进行比对从而达到进行最佳比较的目的(例如,为了在所述的序列间进行最佳的比较,可以向进行比较的所述序列中的任何一个中导入缺口)。之后对处于相对应的氨基酸位置或者核苷位置处的氨基酸残基或者核苷进行比较。当所述的第一个序列中的一个位置被一种氨基酸残基或者核苷占据,而所述的氨基酸残基或者核苷与所述的第二个序列中的相对应的位置处的氨基酸残基或者核苷相同时,那么在这个位置上所述的分子是同源的(即,在这里所使用的氨基酸或者核酸的“同源性”等同于氨基酸或者核酸的“同一性”)。
可以利用两个序列之间的同一性的程度来确定所述的核酸序列的同源性。可以使用本领域已知的计算机程序对所述的同源性进行确定,所述的计算机程序是例如由GCG程序包提供的GAP软件。参见,Needleman以及Wunsch于1970年在J Mol Biol《分子生物学杂志》48:443-453中发表的文章。使用GCG GAP软件并且进行下述的设定以进行核酸序列的比较:空位罚分(gap creation penalty)为5.0并且空位拓展罚分(gap extensionpenalty)为0.3,上述提及的所述相似的核酸序列的编码区域展现出的同一性的程度优选至少为70%,75%,80%,85%,90%,95%,98%,或者99%,其中所述的DNA序列的所述编码序列(编码)如表格1中所示。
所述的术语“序列同一性”指的是在一段特定的比较区域内,两个多核苷酸序列或者多肽序列就每个残基而言是相同的的程度。所述的术语“序列同一性的百分数”是通过下述方式进行计算的:在进行比较的区域内对两个进行最佳比对的序列进行比较,确定同时在两个序列中出现的所述相同的核酸碱基(例如,对于核酸而言,为A,T,C,G,U,或者I)的位置的数量,从而获得所述的匹配位置的数量,将所述的匹配位置的数量除以位于所述的比较区域内的位置的总数量(即,窗口的大小),并且将上述得到的结果乘以100,从而获得所述的序列同一性的百分数。这里所使用的所述术语“本质上的同一性”代表的是一种多核苷酸序列的性质,其中所述的多核苷酸包括一种序列,所述的序列与一种参考序列相比,在比较区域内具有至少80%的序列同一性,优选具有至少85%的序列同一性并且通常具有90%至95%的序列同一性,更加通常具有至少99%的序列同一性。所述的术语“正向残基(positive residue)的百分含量”是通过下述方式进行计算的:在进行比较的区域内对两个进行最佳比对的序列进行比较,如上文中所定义,确定同时在两个序列中出现的所述相同的以及保守的氨基酸取代的位置的数量,从而获得所述的匹配位置的数量,将所述的匹配位置的数量除以位于所述的比较区域内的位置的总数量(即,窗口的大小),并且将上述得到的结果乘以100,从而获得所述的正向残基的百分含量。
重组表达载体以及宿主细胞
本发明的另外一个方面是关于载体的,优选是表达载体,所述的载体含有一种核酸,所述的核酸编码一种MYB-亚群14蛋白、基因、其类似物或者同系物。所述的用以编码MYB-亚群14多肽的序列可以是一种基因组序列或者是一种cDNA序列。这里所使用的所述术语表达载体包括那些被设计成用来提供所述的核酸序列的转录的载体。所述的经过转录的核酸可以被翻译成一种多肽产品或者蛋白产品。这里所使用的所述术语“载体”指的是一种核酸分子,所述的核酸分子能够将另外一种核酸运送到与其进行连接的位置处。一种类型的载体是“质粒”,它指的是一种环形的双链DNA闭合环,可以在其中进行另外的DNA片段的连结。另外一种类型的载体是一种病毒载体,其中另外的DNA片段可以在所述的病毒基因组中进行连结。某些载体能够在它们导入的宿主细胞中进行自主的复制(例如,细菌载体具有一种用于复制的细菌起源)。其他的载体通过向所述的宿主细胞中的导入作用,被整合到所述的宿主细胞的基因组中,并且因而沿所述的宿主基因组进行复制。而且,某些载体能够将所述基因的表达导向与它们进行可操作性的连接的位置处。这样的载体在这里被称为“表达载体”。一般而言,在重组DNA技术中有效利用的表达载体通常是以质粒的形式存在的。在本说明书中,由于所述的质粒是载体的最常见的使用形式,“质粒”以及“载体”可以进行互换使用。然而,本发明意在包括那些其他类型的表达载体,例如病毒载体或者植物转化载体,二元载体(binary)或者另外的能够提供同等功能的载体。
本发明中所述的重组表达载体包括本发明所述的核酸,其中所述的核酸以一种适合于所述的核酸在宿主细胞中进行表达的形式存在,这意味着所述的重组表达载体包括一个或者多个调控序列,它是以需要用来进行表达的所述宿主细胞为依据进行选择的,所述的调控序列与需要进行表达的所述核酸序列进行了可操作性的连接。在一个重组表达载体中,“可操作性的连接”意在表明所述的目标核苷酸序列与所述的调控序列进行了连接,所述的连接是以一种允许所述的核苷酸序列进行表达的方式进行的(例如,在一种体外转录/翻译体系中或者在一种宿主细胞中,当所述的载体被导入到所述的宿主细胞中时)。
所述的术语“调控序列”意在包括启动子,增强子以及其他的表达控制元件(例如,多聚腺苷酸化作用信号)。这样的调控序列在例如下述文献中进行了描述:Goeddel于1990年发表的文章GENE EXPRESSION TECHNOLOGY:METHODS IN ENZYMOLOGY《基因表达技术:酶学方法》185,Academic Press(学术出版社),San Diego(圣地亚哥),Calif.(加利福尼亚)。调控序列包括那些指导核苷酸序列在许多类型的宿主细胞中进行组成性表达的序列,以及那些指导所述的核苷酸序列仅在某些宿主细胞中(例如,组织特异性调控序列)或者在诱导型启动子中进行表达的序列(例如,诱导对于非生物因素的应答以及对于生物的应答,其中所述的非生物因素是例如所述细胞的环境条件,热,干旱,营养状态或者生理学状态,其中所述的生物因素是例如病原体应答性的)。适合的启动子的例子包括例如组成型启动子,脱落酸(ABA)诱导型启动子,组织特异性启动子以及非生物性或者生物性诱导型启动子。本领域技术人员将能够预期到,对于所述的表达载体的设计能够取决于下列因素例如对于需要进行转化的所述宿主细胞的选择,所期望的蛋白表达水平以及时机和表达位置,等等。可以将本发明所述的表达载体导入到宿主细胞中从而制成蛋白或者肽,包括融合蛋白或者肽,其中所述的蛋白或者肽是由本发明所描述的核酸进行编码的(例如,MYB-亚群14蛋白例如MYB68蛋白,MYB68蛋白的突变体形式,融合蛋白,等等)。
可以对本发明中所述的重组表达载体进行设计,使其能够使MYB-亚群14基因或者MYB-亚群14蛋白在原核细胞或者真核细胞中进行表达。例如,MYB-亚群14基因或者MYB-亚群14蛋白可以在细菌细胞、昆虫细胞(使用杆状病毒表达载体)、酵母细胞、植物细胞或者哺乳动物细胞中进行表达,其中所述的细菌细胞是例如大肠杆菌。适合的宿主细胞在下述文献中进行了进一步的讨论:Goeddel于1990年发表的文章GENE EXPRESSION TECHNOLOGY:METHODS IN ENZYMOLOGY《基因表达技术:酶学方法》185,Academic Press(学术出版社),San Diego(圣地亚哥),Calif.(加利福尼亚)。可以选择的,所述的重组表达载体可以在体外进行转录以及翻译,例如使用T7启动子调控序列以及T7聚合酶。
在一种实施方式中,使用一种植物表达载体使本发明所述的核酸在植物细胞中进行表达。植物表达载体系统的例子包括在农杆菌(Agrobacterium)中发现的肿瘤诱导(Ti)质粒或者是上述质粒的一部分,花椰菜花叶病毒(CAMV)DNA以及例如pBI121这样的载体,pCAMBA种类的载体或者本领域技术人员优先选择的一种载体。
为了在植物中进行表达,除了MYB-亚群14多核苷酸之外,所述的重组表达盒中还将含有能够在植物细胞中发挥功效的启动子区域,转录开始位点(如果需要进行转录的所述编码序列缺少位点的话),以及转录终止/多聚腺苷酸化作用序列。所述的终止/多聚腺苷酸化作用区域可以从与所述的启动子序列相同的基因中获得,或者可以从不同的基因中获得。存在于所述的表达盒的5’端以及3’端处的独特的限制酶位点通常被包括在内,从而能够容易的插入到一种预先存在的载体当中。
适合的植物可表达性启动子的例子包括来自于植物病毒的启动子,例如所述的来自于花椰菜花叶病毒(CaMV)的35S启动子(参见Odell等人于1985年在Nature《自然》313:810-812中发表的文章),来自于基因的启动子例如水稻肌动蛋白(参见McElroy等人于1990年在Plant Cell《植物细胞》163-171中发表的文章),泛素(参见Christensen等人于1992年在Plant Mol.Biol.《植物分子生物学》12:619-632中发表的文章;以及Christensen等人于1992年在Plant Mol.Biol.《植物分子生物学》18:675-689中发表的文章),pEMU(参见Last等人于1991年在Theor.Appl.Genet.《理论及应用遗传学》81:581-588中发表的文章),甘露碱合成酶基因启动子(MAS)(参见Velten等人于1984年在EMBO J.《欧洲分子生物学学会杂志》3:2723-2730中发表的文章),玉米H3组蛋白(参见Lepetit等人于1992年在Mol.Gen.Genet.《分子遗传学与普通遗传学》231:276-285中发表的文章;以及Atanassvoa等人于1992年在Plant Journal《植物学杂志》2(3):291-300中发表的文章),来自于根癌农杆菌(Agrobacterium tumefaciens)的T-DNA的5’-或者3’-启动子,Smas启动子,杜仲肉桂醇脱氢酶启动子(美国专利No.5683439),胭脂氨酸合成酶(Nos)启动子,二磷酸核酮糖羧化酶/加氧酶(rubisco)启动子,富甘氨酸蛋白质基因(GRP)-18启动子,ALS启动子(WO 96/30530),合成启动子,例如Rsyn7启动子,单细胞蛋白(S CP)启动子以及偶联蛋白(UCP)启动子,1,3-二磷酸核酮糖羧化酶,果实特异性启动子,热休克启动子,种子特异性启动子以及其他来自于各种不同的植物基因的转录开始区域,例如,包括各种不同的opine开始区域,例如,章鱼碱,甘露碱,以及胭脂氨酸。有用的启动子同样包括热诱导型启动子例如所述的热休克蛋白(HSP)18.2启动子或者热休克蛋白(HSP)81.1启动子(参见Takahashi等人于1992年在Plant J.《植物学杂志》2,751-761中发表的文章;Yoshida等人于1995年在Appl.Microbiol.Biotechnol.《应用微生物学与生物技术》44,466-472中发表的文章;Ueda等人于1996年在Mol Gen Genet《分子遗传学与普通遗传学》250:533-539中发表的文章)。潜在启动子同样能够有效的用于本发明中可以使用的嵌合构建体。当潜在基因调控元件存在于它们在所述的基因组中的本来位置处时,是不具备活性的,但是当将其放置于邻近转基因植物的基因位置处时,它们具有完全的功能。
除了嵌合启动子-基因构建体之外,可以预期到天然存在的MYB-亚群14启动子的用途。通过调控元件的纳入,可以对一种天然启动子的表达性质进行修饰,这样一来表达水平被提升和/或进行异位的表达和/或进行组成性的表达。例如,可以在一种构建体中纳入四个(4X)35S增强子序列(参见Wiegel等人于2000年发表的文章),用以增强表达。或者,可以利用一种含有四个35S增强子序列的构建体进行转化,从而制备一类植物群体,其中所述的构建体是例如Wiegel等人于2000年发表的文章中所描述的pSKI 15载体。可以对上述经过转化的群体进行筛选,从而筛选出具有增强的MYB-亚群14序列的表达的植物,或者筛选出具有增强的热耐受性以及减少的花朵败育的植物,或者进行这类筛选的组合,从而对目标植物进行识别。
为了在植物细胞中进行表达,能够与编码MYB-亚群14的核酸序列进行连接的其他的调控元件包括终止子,多聚腺苷酸化作用序列,以及编码信号肽的核酸序列,其允许存在于植物细胞中或者将所述的蛋白从所述的细胞中分泌出来。这样的调控元件以及将这些元件加入到MYB-亚群14基因的调控元件之中或者利用这些元件与MYB-亚群14基因中的调控元件进行交换的方法是本领域已知的,并且包括,但不局限于,3’终止和/或多聚腺苷酸化作用区域例如所述的根癌农杆菌(Agrobacterium tumefaciens)胭脂氨酸合成酶(nos)基因中的3’终止和/或多聚腺苷酸化作用区域(参见Bevan等人于1993年在Nucl.Acids Res.《核酸研究》12:369-385中发表的文章);马铃薯蛋白酶抑制剂II(PINII)基因中的3’终止和/或多聚腺苷酸化作用区域(参见Keil等人于1986年在Nucl.Acids Res.《核酸研究》14:5641-5650中发表的文章并且在此被引入作为参考;以及An等人于1989年在Plant Cell《植物细胞》1:115-122中发表的文章);以及所述的花椰菜花叶病毒19S基因中的3’终止和/或多聚腺苷酸化作用区域(参见Mogen等人于1990年在Plant Cell《植物细胞》2:1261-1272中发表的文章)。
植物信号序列,包括,但不局限于,编码信号肽的DNA/RNA序列,所述的序列将蛋白靶向定位于(target proteins to)所述的植物细胞的细胞外基质(参见Dratewka-Kos等人于1989年在J.Biol.Chem.《生物化学杂志》264:4896-4900中发表的文章)以及所述的皱叶烟草(Nicotiana plumbaginifolia)的延伸基因(参见DeLoose等人于1991年在Gene《基因》99:95-100中发表的文章),或者所述的信号肽将蛋白靶向定位于所述的液泡,如所述的甘薯贮藏蛋白基因(参见Matsuka等人于1991年在Proc.Nat’1Acad.Sci.(USA)《美国科学院院刊》88:834中发表的文章)以及所述的大麦凝集素基因(参见Wilkins等人于1990年在PlantCell《植物细胞》2:301-313中发表的文章),或者所述的信号能够引起蛋白的分泌例如PRIb的信号(参见Lind等人于1992年在Plant Mol.Biol.《植物分子生物学》18:47-53中发表的文章),或者是那些将蛋白靶向定位于所述的质体的信号例如油菜籽脂烯酰基酰基载体蛋白(enoyl-ACP)还原酶的信号,这些都可以有效的用于本发明之中。
在另外一种实施方式中,所述的重组表达载体能够指导所述的核酸优先在一种特定的细胞类型中进行表达(例如,组织特异性调控元件被用来进行所述核酸的表达)。组织特异性调控元件是本领域已知的。能够与本发明中所述的核酸进行特别有效的连接的是表达系统,所述的表达系统可以在植物中进行操作。这些系统包括受到组织特异性启动子的控制的系统,以及那些包括能够在所有的植物组织中进行操作的启动子的系统。
器官特异性启动子同样是熟知的。例如,所述的查尔酮合成酶-A基因(参见vander Meer等人于1990年在Plant Molecular Biology《植物分子生物学》15(1):95-109中发表的文章)或者所述的二氢黄酮醇-4-还原酶(dfr)启动子(参见Elomaa等人在The PlantJournal《植物学杂志》16(1)93-99中发表的文章)能够在特异性的花朵组织中指导表达。同样可以利用的是所述的patatin I类启动子,所述的启动子仅仅能够在所述的马铃薯块茎中发生转录活化,并且可以被用来作用于在所述的块茎中发生的基因表达(参见Bevan M.于1986年在Nucleic Acids Research《核酸研究》14:4625-4636中发表的文章)。另外一种马铃薯特异性启动子是所述的颗粒结合型淀粉合成酶(GBSS)启动子(参见Visser R.G.R.等人于1991年在Plant Molecular Biology《植物分子生物学》17:691-699中发表的文章)。
可以使用已知的步骤对其他的器官特异性启动子进行分离,其中所述的器官特异性启动子对于一种目标靶向器官而言是适合的。这些控制序列通常是与一类基因相关联的,这类基因能够在所述的目标器官中进行唯一的表达。在一种典型的高等植物中,每个器官中含有数以千计的mRNA,这些mRNA在其他的器官系统中是不存在的(参见Goldberg P.于1986年在Trans.R.Soc.London B《皇家学会哲学汇刊,B辑》314:343中发表的文章)。
上述得到的表达系统或者表达盒被连结在或者另外通过进行构建而被纳入到一种重组载体之中,其中所述的重组载体是适于进行植物的转化的。所述的载体中还可以含有选择性标记基因,通过所述的选择性标记基因,可以在培养中对经过转化的植物细胞进行识别。所述的标记基因可以编码抗生素抗性。这些标记包括对于G418的抗性,对于潮霉素的抗性,对于博来霉素的抗性,对于卡那霉素的抗性,以及对于庆大霉素的抗性。或者所述的标记基因能够编码一种具有除草剂耐受性的基因,从而提供对于草铵膦类型或者草甘膦类型的除草剂的耐受性。在对所述的植物细胞进行转化之后,通过其能够在含有所述的特定抗生素或者除草剂的培养基中进行生长的能力,将能够识别出那些含有所述的载体的细胞。起源于细菌或者病毒的复制序列通常也是被包括在内的,从而允许所述的载体在一种细菌宿主或者噬菌体宿主中进行克隆,优选被包括在内的是一种具有广泛的宿主范围的起源于原核的复制。一种细菌的选择性标记同样也应当被包括在内,从而允许对承载了所述的目标构建体的细菌细胞进行选择。适合的原核选择性标记同样包括对于抗生素的抗性,其中所述的抗生素是例如卡那霉素或者四环素。
正如本领域中所已知的,用于编码额外功能的其他DNA序列同样可以存在于所述的载体之中。例如,在进行农杆菌(Agrobacterium)转化的情形中,为了随后向植物染色体中进行转移,T-DNA序列同样也应当被包括在内。
本发明的另外一个方面是关于宿主细胞的,在所述的宿主细胞中导入本发明中所述的重组表达载体。所述的术语“宿主细胞”以及“重组宿主细胞”在本文中可以互换使用。可以理解的是,这些术语不仅仅指的是具体的宿主细胞,其同样指代所述的这种细胞的后裔或者潜在的后裔。由于突变或者环境影响的原因,在接下来的世代中可能发生某些修饰,这使得这样的后裔实际上不同于所述的母本细胞,但其仍然被包括在这里所使用的所述术语的范围之内。
可以经由传统的转化技术或者转染技术,将载体DNA导入到原核细胞或者真核细胞之中。这里所使用的所述术语“转化”以及“转染”意在指代本领域认知的用于向一种宿主细胞中导入一种外源核酸(例如,DNA)的各种技术。
本发明中所述的宿主细胞,例如在培养之中的原核宿主细胞或者真核宿主细胞,可以被用来制备(即,表达)本发明所述的多肽,其中所述的多肽是在本发明中所述的多核苷酸的开放阅读框中进行编码的。因此,本发明进一步提供用于制备一种多肽的方法,其中所述的方法使用了本发明中所述的宿主细胞。在一种实施方式中,所述的方法包括在一种适合的培养基中培养本发明中所述的宿主细胞(向所述的宿主细胞中导入编码本发明所述的多肽的重组表达载体),从而制备出所述的多肽。在另外一种实施方式中,所述的方法进一步包括从所述的培养基或者所述的宿主细胞中分离所述的多肽。
许多类型的细胞可以作为适合的宿主细胞被用于进行多肽的表达,其中所述的多肽是由本发明中所述的多核苷酸的开放阅读框进行编码的。植物宿主细胞包括,例如,能够发挥适合的宿主功能、用于进行本发明中所述的多核苷酸的表达的植物细胞包括来自于各种不同的植物种类的表皮细胞,叶肉以及其他的基本组织,以及叶片中的脉管组织,茎,花朵器官,以及根,其中所述的各种不同的植物种类是例如拟南芥属(Arabidopsis),芸薹属(Brassica),稻属(Oryza),玉蜀黍属(Zea),高粱(Sorghum),棉花属(Gossypium),小麦属(Triticum),豆属(Glycine),豌豆(Pisum),菜豆属(Phaseolus),番茄属(Lycopersicon),三叶草(Trifolium),大麻(Cannabis),南瓜(Cucurbita),蔷薇(Rosa),葡萄(Vitis),胡桃(Juglans),草莓(Fragaria),莲花(Lotus),苜蓿(Medicago),驴豆(Onobrychis),葫芦巴(Trigonella),豇豆(Vigna),柑橘属(Citrus),亚麻属(Linum),天竺葵(Geranium),木薯(Manihot),胡萝卜属(Daucus),萝卜属(Raphanus),芥子属(Sinapis),颠茄属(Atropa),辣椒属(Capsicum),曼陀罗(Datura),莨菪属(Hyoscyamus),烟草(Nicotiana),茄属(Solanum),矮牵牛(Petunia),洋地黄(Digitalis),墨角兰(Majorana),菊苣属(Cichorium),向日葵(Helianthus),莴苣属(Lactuca),雀麦属(Bromus),芦笋(Asparagus),金鱼草属(Antirrhinum),萱草属(Hemerocallis),Nemesis,天竺葵属(Pelargonium),黍樱(Panieum),狼尾草(Pennisetum),毛茛属(Ranunculus),千里光(Senecio),蛾蝶花(Salpiglossis),黄瓜属(Cucumis),蓝英花(Browallia),黑麦草属(Lolium),燕麦属(Avena),大麦属(Hordeum),黑麦属(Secale),云杉属(Picea),Caco,以及杨属(Populus)。
保守性突变
除了可能存在于所述群体之中的MYB-亚群14或者MYB68序列的天然发生的等位基因突变体之外,所述的熟练技术人员将能够进一步的预期到,可以通过突变方式向具有下述序列识别号的核苷酸序列中导入变化,所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265,从而使所述的经过编码的MYB-亚群14或者MYB68蛋白的氨基酸序列发生变化,并且不改变所述的MYB-亚群14或者MYB68蛋白的功能性能力。例如,可以在具有下述序列识别号的序列中利用核苷酸取代引发在“非必需”氨基酸残基上的氨基酸取代,所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264。“非必需”氨基酸残基指的是这样的一种残基,其可以从所述的MYB-亚群14或者MYB68野生型序列中发生改变,但不会改变所述的生物活性,而“必需”氨基酸残基是生物活性所需要的。例如,存在于本发明所述的MYB-亚群14或者MYB68蛋白中的保守的氨基酸残基被预测为是不理想的改变性质的候选者。对于保守性区域的比对以及识别在本发明中进行了描述,并且这种比对以及识别为必需氨基酸以及保守性氨基酸的识别提供了进一步的指导。
本发明的另外一个方面是关于核酸分子的,其中所述的核酸分子编码一种MYB-亚群14或者MYB68蛋白,在所述的MYB-亚群14或者MYB68蛋白中存在着氨基酸残基上的变化,这些氨基酸残基对于活性而言不是必需的。这样的MYB-亚群14或者MYB68蛋白与具有下列序列识别号的序列的氨基酸序列存在不同但仍然保留了生物学活性,其中所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264。在一种实施方式中,所述的被分离的核酸分子包括一种编码蛋白的核苷酸序列,其中所述的蛋白包括一种氨基酸序列,所述的氨基酸序列与具有下述序列识别号的氨基酸序列存在至少大约75%的同源性,其中所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264。优选的,由所述的核酸编码的所述蛋白与具有下述序列识别号的序列存在至少大约80%的同源性,其中所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264,更加优选与具有下述序列识别号的序列具有至少大约90%、95%、98%、并且最优选具有至少大约99%的同源性,其中所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264。
一种经过分离的核酸分子,所述的核酸分子编码一种MYB-亚群14或者MYB68蛋白,其中所述的MYB-亚群14或者MYB68蛋白与具有下述序列识别号的蛋白是同源的,其中所述的序列为:序列2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,39,41,43,45,47,49,51,53,55,57,60,62,64,66,68,70,72,74,76,78,80,82,84,86,89,93,95,97,99,101,103,105,107,111,113,115,117,119,121,123,126,128,131,133,136,138,140,142,144,146,148,150,152,154,157,159,161,163,165,167,169,171,173,175,177,179,181,183,185,187,189,191,193,195,197,199,201,203,205,207,209,212,215,219,221,223,225,227,229,231,233,235,237,239,241,243,245,248,250,252,254,256,258,260,262以及264,这样的核酸分子可以通过向具有下述序列识别号的核苷酸序列中导入一个或者多个核苷酸的取代、加成或者缺失来制备,其中所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265,这样一来一个或者多个氨基酸的取代、加成或者缺失被导入到所述被编码的蛋白之中。
可以通过标准的技术向具有下述序列识别号的核苷酸序列中导入突变,所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265,其中所述的标准技术是例如定点突变以及由聚合酶链式反应(PCR)介导的突变。优选的,在一个或者多个预测的非必需氨基酸残基处进行保守性氨基酸的取代。“保守性氨基酸取代”指的是利用一种具有相似的侧链的氨基酸残基对所述的氨基酸残基进行取代。具有相似的侧链的氨基酸残基家族已经在所属领域中进行了定义。这些家族包括具有碱侧链的氨基酸(例如,赖氨酸,精氨酸,组氨酸),具有酸侧链的氨基酸(例如,天冬氨酸,谷氨酸),具有不带电荷的极性侧链的氨基酸(例如,甘氨酸,天冬酰胺,谷氨酰胺,丝氨酸,苏氨酸,酪氨酸,半胱氨酸),具有非极性侧链的氨基酸(例如,丙氨酸,缬氨酸,亮氨酸,异亮氨酸,脯氨酸,苯丙氨酸,甲硫氨酸,色氨酸),具有β-分支侧链的氨基酸(例如,苏氨酸,缬氨酸,异亮氨酸)以及具有芳香族侧链的氨基酸(例如,酪氨酸,苯丙氨酸,色氨酸,组氨酸)。因此,利用与存在于MYB68中的预测的非必需氨基酸残基属于相同的侧链家族的另外一种氨基酸残基对所述的非必需氨基酸残基进行取代。可以选择的,在另外一种实施方式中,可以沿所述的MYB-亚群14或者MYB68编码序列的全部或者一部分随机的导入突变,例如通过饱和突变的方式,并且可以对上述得到的突变体进行生物活性的筛选,从而识别出保留了活性以及所期望的表现型的突变体。在具有下述序列识别号的序列发生突变之后,可以利用本领域已知的任意重组技术对所述被编码的蛋白进行表达,并且可以确定所述蛋白的活性,其中所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。
经过转化的植物细胞以及转基因植物
本发明包括原生质体,植物细胞,植物组织以及植物(例如,单子叶植物或者双子叶植物),其中所述的原生质体,植物细胞,植物组织以及植物(例如,单子叶植物或者双子叶植物)经过了MYB-亚群14核酸、含有MYB-亚群14核酸的载体或者含有MYB-亚群14核酸的表达载体的转化。这里所使用的“植物”不仅仅意在包括整个的植物,同时也包括所述植物的某个部分(即,细胞,以及组织,包括例如,叶片,茎,嫩枝,根,花朵,果实以及种子)。
所述的植物可以是任意种类的植物,包括,例如,选自下列种属的物种:拟南芥属(Arabidopsis),芸薹属(Brassica),稻属(Oryza),玉蜀黍属(Zea),高粱(Sorghum),棉花属(Gossypium),小麦属(Triticum),豆属(Glycine),豌豆(Pisum),菜豆属(Phaseolus),番茄属(Lycopersicon),三叶草(Trifolium),大麻(Cannabis),南瓜(Cucurbita),蔷薇(Rosa),葡萄(Vitis),胡桃(Juglans),草莓(Fragaria),莲花(Lotus),苜蓿(Medicago),驴豆(Onobrychis),葫芦巴(Trigonella),豇豆(Vigna),柑橘属(Citrus),亚麻属(Linum),天竺葵(Geranium),木薯(Manihot),胡萝卜属(Daucus),萝卜属(Raphanus),芥子属(Sinapis),颠茄属(Atropa),辣椒属(Capsicum),曼陀罗(Datura),莨菪属(Hyoscyamus),烟草(Nicotiana),茄属(Solanum),矮牵牛(Petunia),洋地黄(Digitalis),墨角兰(Majorana),菊苣属(Cichorium),向日葵(Helianthus),莴苣属(Lactuca),雀麦属(Bromus),芦笋(Asparagus),金鱼草属(Antirrhinum),萱草属(Hemerocallis),Nemesis,天竺葵属(Pelargonium),黍樱(Panieum),狼尾草(Pennisetum),毛茛属(Ranunculus),千里光(Senecio),蛾蝶花(Salpiglossis),黄瓜属(Cucumis),蓝英花(Browallia),黑麦草属(Lolium),燕麦属(Avena),大麦属(Hordeum),黑麦属(Secale),云杉属(Picea),Caco,以及杨属(Populus)。
本发明同样包括细胞,组织,包括例如,叶片,茎,嫩枝,根,花朵,果实以及种子以及来自于所述的转化植物的后裔。
用于将外源基因向植物中导入的许多方法均是已知的并且可以利用上述方法将基因插入到一种植物宿主中,包括生物学以及物理学植物转化方案(参见,例如,Miki等人于1993年在Methods in Plant Molecular Biology and Biotechnology《植物分子生物学与生物技术的方法》中发表的文章“Procedure for Introducing Foreign DNA intoPlants《向植物中导入外源DNA的步骤》”,Glick以及Thompson编辑,CRC Press Inc.,BocaRaton,第67-88页;以及Andrew Bent于1998年在Clough SJ and Bent AF中发表的文章“Floral dipping:a simplified method forAgrobacterium-mediated transformationof Arabidopsis thaliana《花朵的浸泡:一种对拟南芥进行农杆菌介导的转化的简便方法》”)。根据所述的宿主植物的不同来选择所述的方法,并且所述的方法包括化学转染法例如磷酸钙,聚乙二醇(PEG)转化法,由微生物例如农杆菌(Agrobacterium)介导的基因转移(参见Horsch等人于1985年在Science《科学》227:1229-31中发表的文章),电穿孔,原生质体转化法,显微注射,花朵浸泡(flower dipping)以及基因枪轰击法。
由农杆菌(Agrobacterium)介导的转化
用于向植物中导入表达载体的应用最广泛的方法是以所述的根癌农杆菌(Agrobacterium tumefaciens)以及发根农杆菌(A.rhizogenes)的天然转化系统为基础的,其中所述的根癌农杆菌以及发根农杆菌是能够对植物细胞进行遗传性转化的植物致病细菌。根癌农杆菌以及发根农杆菌的肿瘤诱导(Ti)质粒以及根诱导(Ri)质粒携带有进行植物的遗传性转化的基因(参见,例如,Kado于1991年在Crit.Rev.Plant Sci.《植物科学的评论与回顾》10:1-32中发表的文章)。在前述的Gruber等人发表的文章中以及Moloney等人于1989年在Plant Cell Reports《植物细胞学报告》8:238-242中发表的文章中提供了对于农杆菌载体系统以及由农杆菌介导的基因转移的说明。
根据Andrew Bent于1998年在Clough SJ and Bent AF中发表的文章Floraldipping:a simplified method for Agrobacterium-mediated transformation ofArabidopsis thaliana《花朵的浸泡:一种对拟南芥进行农杆菌介导的转化的简便方法》中所描述的方法,通过在农杆菌(Agrobacterium)培养液中浸泡开花植物的方法,能够容易的制备出转基因拟南芥(Arabidopsis)植物。使野生型植物进行生长,直至所述的植物孕育花朵并且开花。将所述的植物倒转放入农杆菌培养液溶液中保持1分钟,其中所述的农杆菌培养液溶液中带有所述的适合的基因构建体。之后将植物水平放置于盘中并且保持覆盖两天,从而维持湿度,并且随后将其扶正(righted)并且装入袋中(bagged)以继续进行生长以及种子的发育。大批量的收获成熟的种子。
直接的基因转移
一种通常可以应用的植物转化的方法是由微弹介导的转化,其中在所述的微弹的表面上携带着DNA,所述的微弹具有大约1至4微米的测量尺寸。将所述的表达载体导入到植物组织中,利用基因枪装置对所述的微弹进行加速,使其达到300至600米/秒的速度,所述的速度足以穿透所述的植物细胞壁以及细胞膜(参见Sanford等人于1987年在Part.Sci.Technol.5:27-37中发表的文章;Sanford于1988年在Trends Biotech《生物技术趋势》6:299-302中发表的文章;Sanford于1990年在Physiol.Plant《植物生理学》79:206-209中发表的文章;Klein等人于1992年在Biotechnology《生物技术》10:286-291中发表的文章)。
还可以通过美国专利5240842、美国专利6809232中描述的气雾剂束注射(ABI)方法来完成植物的转化。使用气雾剂束技术对湿颗粒或者干颗粒进行加速,使得所述的颗粒能够穿透存活的细胞。气雾剂束技术利用到惰性气体的射流膨胀,使其经由一个小孔从具有较高的气体压力的区域流向具有较低的气体压力的区域。所述的膨胀的气体加速了气雾剂液滴,其中所述的气雾剂液滴中含有需要被导入到细胞或者组织当中的核酸分子。对所述的加速颗粒进行放置使其冲击一个优选的靶向,例如一种植物细胞。所述的颗粒作为一个具有足够小的尺寸的液滴被进行构建,从而使所述的细胞经过穿透而存活下来。利用所述的应用领域中已知的标准技术使所述的经过转化的细胞或者组织进行生长从而制备一种植物。
转化体的再生
从单一的植物原生质体或者从各种不同的外植体中进行植物的发育或者再生是本领域熟知的(参见Weissbach以及Weissbach于1988年发表的文章)。这种再生以及生长的过程通常包括下述步骤:转化细胞的筛选,对那些个体细胞进行培养,经由所述的胚胎发育的平常时期并且经由所述的根苗时期。转基因晶胚以及种子进行类似的再生。在此后将上述得到的转基因发根嫩芽种植在一种适宜的植物生长培养基例如土壤中。
所述的植物的发育或者再生可以利用已经有所描述的本领域熟知的方法(参见Horsch等人于1985年发表的文章)来完成,其中所述的植物中含有编码目标多肽的所述的外源、外生性基因,其中所述的基因是由来自于叶片外植体的农杆菌(Agrobacterium)导入的。在这个方法中,将所述的转化体在存在选择性试剂的条件下进行培养,并且利用已经有所描述的、能够诱发所述的被转化植株的嫩枝进行再生的培养基(参见Fraley等人于1983年发表的文章)中对所述的转化体进行培养。具体的,美国专利No.5349124(说明书在本文中被引入作为参考)详细描述了遗传转化的莴苣细胞的建立以及由此得到的植物,所述的植物表达杂交的晶体蛋白,其中所述的晶体蛋白为所述的植物赋予杀虫的活性,其能够抵抗鳞翅目的幼虫。
这种方法通常在两个月至四个月的时间内制备出嫩枝并且随后将那些嫩枝转移到一种适宜的根诱导培养基中,其中所述的根诱导培养基中含有所述的选择性试剂以及一种抗生素用于预防细菌的生长。在所述的选择性试剂的存在下,嫩枝发根从而形成苗,之后将苗移植到土壤或者其他介质中从而允许根部的生成。这些过程依据所使用的具体的植株而发生改变,这些改变是本领域熟知的。
优选的,所述的再生型植物是自花授粉的从而提供了纯合的转基因植物,或者将从所述的再生型植物中获得的花粉交叉传授给具有农艺学重要性的种子生长型植物,所述的种子生长型植物优选是近交系的。相反的,将来自于那些重要品系的植物的花粉用于对再生型植物进行授粉。使用本领域技术人员熟知的方法对含有目标多肽的本发明所述的转基因植物进行栽植。
一种优选的转基因植物是一种独立的分离株(segregant)并且能够将所述的MYB68基因及其活性传递给它的后裔。一种更加优选的转基因植物对于所述的基因而言是纯合的,并且能够将这种基因传递给有性交配的所有后代。转基因植物的种子可以在野外生长或者在温室中生长,并且得到的性成熟的转基因植物是自花授粉的从而生成真正的育种植物。这些植物的后裔成为真正的育种品系,这可以通过所述的MYB68转基因的表达的增强来进行评价。
制备转基因植物的方法
制备转基因植物的方法是包含在本发明之内的。所述的方法包括向一个或者多个植物细胞中导入一种化合物,用以对所述植物中的MYB-亚群14的表达或者活性进行改变,从而生成一种转基因植物细胞,以及从所述的转基因细胞中进行转基因植物的再生。所述的化合物增强了MYB-亚群14的表达或者活性。可以对所述的增强的表达和/或活性进行另外的指导,使其以异位的方式或者以组成型的方式或者以组织特异性的方式发生。所述的化合物可以是,例如,(i)MYB-亚群14多肽;(ii)MYB-亚群14核酸及其类似物以及同系物;(iii)能够增强MYB-亚群14核酸的表达的核酸。能够增强MYB-亚群14核酸的表达的核酸可以包括启动子元件或者增强子元件。所述的启动子是一种异源启动子或者是一种同源启动子。除此之外,所述的启动子是一种组成型启动子或者是一种诱导型启动子。启动子包括例如,器官特异性启动子或者组织特异性启动子。适合指导基因表达的启动子是本领域已知的并且在本发明进行了描述。增强子元件对于本领域技术人员而言是已知的。例如所述的增强子元件是一种35S增强子元件。
通过增加所述的MYB亚群-14多肽的表达,意味着由所述的经过所述核酸构建体进行转化的细胞所生产的量高于没有经过所述的核酸构建体进行转化的细胞所生产的量,其中所述的没有进行转化的细胞是例如一种对照细胞。对照细胞包括例如一种能够内源性的表达MYB亚群-14多肽的细胞,例如一种植物根细胞,或者对照细胞是与所述的转化细胞具有相同的细胞类型的一种非转化细胞,是一种叶片细胞、分生组织细胞或者花朵细胞或者种子细胞。增加指的是一倍的增加,两倍的增加,三倍的增加或者更高倍数的增加。表达上的增加同样意在包括在一种通常情况下不产生MYB亚群-14多肽的细胞中进行的MYB亚群-14多肽的表达。
所述的核酸可以是内源性的或者是外源性的。优选的,所述的化合物是一种MYB-亚群14多肽或者编码一种MYB-亚群14多肽的MYB-亚群14核酸。例如所述的化合物包括具有下述序列识别号的核酸序列,所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。优选的,所述的化合物是一个MYB-亚群14核酸序列,其中所述的核酸序列来自于被转化的物种的内源性来源。或者,所述的化合物是一种MYB-亚群14核酸序列,其中所述的核酸序列来自于被转化的物种的外源性来源。
制备转基因植物的方法是同样被包含在本发明之内的。所述的方法包括向一个或者多个植物细胞中导入一种化合物,用以对所述植物中的MYB-亚群14核酸的表达或者活性进行改变,从而生成一种转基因植物细胞,以及从所述的转基因细胞中进行转基因植物的再生。所述的化合物增强了MYB-亚群14序列的表达或者活性。所述的化合物可以是,例如,(i)MYB-亚群14多肽;(ii)MYB-亚群14核酸及其类似物以及同系物;(iii)能够增强MYB-亚群14核酸的表达的核酸。能够增强MYB-亚群14核酸的表达的核酸可以包括启动子元件或者增强子元件。所述的核酸可以是内源性的或者是外源性的。优选的,所述的化合物是一种MYB-亚群14多肽或者MYB-亚群14核酸。例如所述的化合物包括具有下述序列识别号的核酸序列,所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。优选的,所述的化合物是一个MYB-亚群14核酸序列,其中所述的核酸序列相对于被转化的物种而言是内源性的。或者,所述的化合物是一种MYB-亚群14核酸序列,其中所述的核酸序列相对于被转化的物种而言是外源性的。
在一种宿主物种中进行表达的外源性MYB-亚群14序列不需要与所述的内源性MYB-亚群14序列相同。例如,以与GAMYB基因的序列相似性为基础,可以获得三个拟南芥GAMYB样基因的序列,其中所述的GAMYB基因来自于大麦,水稻,以及毒麦(L.temulentum)。这三个拟南芥基因被确定为能够编码转录因子(AtMYB33,AtMYB65,以及AtMYB101),并且能够取代大麦GAMYB并且控制α-淀粉酶的表达(参见Gocal等人于2001年在Plant Physiol.《植物生理学》127:1682-1693中发表的文章)。
能够对类黄酮的生物合成进行调控的玉米MYB转录因子、矮牵牛花MYB转录因子以及AtMYB转录因子具有高度的遗传相似性,并且对存在于它们的天然物种中的相同的特点产生影响,因此在这些不同的物种中,这些MYB转录因子的序列以及功能是彼此间相互关联的(参见Borevitz等人于2000年在PlantCell《植物细胞》12:2383-2394中发表的文章)。
因此仅仅需要在所述的宿主细胞中对被表达的MYB-亚群14进行功能性的识别即可。编码核酸的MYB-亚群14在拟南芥中的表达为所述的功能等同(functionallyequivalent)检测提供了基础。例如来自于芸薹属,大豆,棉花或者玉米来源的MYB-亚群14在拟南芥中的表达以及对于所述的热耐受性的评估证明了功能的等同性并且为下述预测提供了可靠的基础:所述的外源性序列是一种MYB-亚群14基因并且因此发挥功能。
本发明中公开的是对于MYB-亚群14序列的表达进行的描述,其中所述的MYB-亚群14序列来自于拟南芥,芸薹属以及大豆,已经证明这种表达在拟南芥中是可以发挥功效的,这是因为上述得到的植物具有增强的热耐受性以及在开花过程中在热胁迫条件下具有增加的结实,其中所述的增强的热耐受性是由减少的花朵败育所表明的。
在各种不同的方面中,与野生型植物(即,未经过转化的)相比,所述的转基因植物具有一种改变的表现型。改变的表现型意味着所述的植物具有一个或者多个不同于所述的野生型植物的特征。例如,当将所述的转基因植物与一种化合物进行接触时,其中所述的化合物能够增强所述的MYB-亚群14核酸的表达或者活性,与野生型植物相比,所述的植物具有一种例如热耐受性增强的表现型,并且在表现型中显现出这种特点例如减少的花朵败育,增加的结实以及发育,增强的产量保护以及对于花粉发育的保护以及分生组织的保护,特别是对于花朵分生组织的保护,保护其免受热损伤,干旱的耐受性以及盐的耐受性。与对照植物相比,具有减少的花朵败育的植物在种子的产量上具有5%,10%,20%,25%,30%或者更高的增长。
所述的植物可以是任意种类的植物,包括,例如,选自下列种属的物种:拟南芥属(Arabidopsis),芸薹属(Brassica),稻属(Oryza),玉蜀黍属(Zea),高粱(Sorghum),棉花属(Gossypium),小麦属(Triticum),豆属(Glycine),豌豆(Pisum),菜豆属(Phaseolus),番茄属(Lycopersicon),三叶草(Trifolium),大麻(Cannabis),南瓜(Cucurbita),蔷薇(Rosa),葡萄(Vitis),胡桃(Juglans),草莓(Fragaria),莲花(Lotus),苜蓿(Medicago),驴豆(Onobrychis),葫芦巴(Trigonella),豇豆(Vigna),柑橘属(Citrus),亚麻属(Linum),天竺葵(Geranium),木薯(Manihot),胡萝卜属(Daucus),萝卜属(Raphanus),芥子属(Sinapis),颠茄属(Atropa),辣椒属(Capsicum),曼陀罗(Datura),莨菪属(Hyoscyamus),烟草(Nicotiana),茄属(Solanum),矮牵牛(Petunia),洋地黄(Digitalis),墨角兰(Majorana),菊苣属(Cichorium),向日葵(Helianthus),莴苣属(Lactuca),雀麦属(Bromus),芦笋(Asparagus),金鱼草属(Antirrhinum),萱草属(Hemerocallis),Nemesis,天竺葵属(Pelargonium),黍樱(Panieum),狼尾草(Pennisetum),毛茛属(Ranunculus),千里光(Senecio),蛾蝶花(Salpiglossis),黄瓜属(Cucumis),蓝英花(Browallia),黑麦草属(Lolium),燕麦属(Avena),大麦属(Hordeum),黑麦属(Secale),云杉属(Picea),Caco,以及杨属(Populus)。
识别热胁迫耐受性植物的方法
同样被包括在本发明之内的是一种识别热胁迫耐受性植物的方法。与一种对照植物相比,通过这些方法识别出的所述植物具有减少的花朵败育以及增加的产量。热胁迫耐受性植物是通过下述方式被识别出来的:将一群开花植物暴露于热胁迫处理中并且从上述的植物群体中选择一种具有减少的花朵败育的植物。热胁迫处理包括例如将所述的植物在足够热的温度下暴露足够长的时间,这样一来导致了对植物的功能或者发育上的损伤。减少的花朵败育意味着植物不会损失与因花朵败育而损失的同样多的花朵,或者与另外一种被暴露于类似的热胁迫水平下的植物相比,所述的植物具有更高的结实产量。与一种对照植物相比,具有减少的花朵败育的植物在结实产量上具有5%,10%,20%,25%,30%或者更高的增长。
实施例
本发明将在下述的非限制性实施例中进行进一步的描述。
实施例1:热耐受性突变体的识别
使用pSKI15载体对拟南芥哥伦比亚生物型(Arabidopsis thalianavar.Columbia)进行转化,其中所述的pSKI15载体中含有四个35S增强子序列(参见Wiegel等人于2000年发表的文章)。从拟南芥生物资源中心(ABRC)处获得T3世代的拟南芥种子并且用来制备T4世代,所述的T4世代被用来进行遗传筛选试验。所述的拟南芥h138突变体被识别出来,当在开花过程中被暴露于大约45℃的热胁迫下保持大约30至60分钟时,与野生型对照相比,所述的突变体具有减少的花朵败育或者不存在花朵败育。通过下述方式对初始的分离进行再测试:使开花植物经受1小时的温度梯度上升,由22℃上升至45℃,随后经受2小时的45℃下的热胁迫,对花朵的产量、结实以及种子的发育进行监控并且对热胁迫图线进行选择。
实施例2:热胁迫MYB68基因的识别
按照下述方法完成基因组的步移,从而使所述的T-DNA活化标签的插入局部化(localize)。通过苯酚:氯仿提取法对基因组DNA进行纯化,其中使用的是10天大的突变体h138的幼苗。随后利用所述的限制酶对上述分离的DNA进行消化,从而生成具有钝末端的DNA片段,其中所述的限制酶是例如EcoRV,PvuII,NruI,或者StuI。将上述来自于每个消化反应的片段与衔接子进行连结,其中所述的衔接子是由两个寡核苷酸(oligos)的退火形成的,其中所述的两个寡核苷酸(oligos)是:衔接子1以及衔接子2。所述的衔接子与所述的DNA片段发生的加成使得能够进行聚合酶链式反应(PCR)扩增,其中使用对所述的衔接子具有特异性的引物以及T-DNA插入位点。
进行两轮聚合酶链式反应(PCR),从而生成用于进行进一步的测序分析的DNA片段。在第一轮聚合酶链式反应中使用引物HeatL1(序列)以及引物CAP1(序列),其中所述的引物HeatL1对所述的T-DNA的左边界具有特异性,而引物CAP1对所述的衔接子具有特异性。将上述得到的聚合酶链式反应的产物稀释50倍,用来作为第二轮聚合酶链式反应的模板。之后利用两个套式引物HeatL2(序列)以及CAP2(序列)对一段确认的DNA片段进行扩增。使用聚合酶链式反应程序TOUCH1(94℃,25秒,6次循环;72℃,7分钟;94℃,25秒,32次循环;67℃,7分钟,以及67℃,10分钟,1次循环)以及TOUCH2(94℃,25秒,4次循环;72℃,7分钟;94℃,25秒,20次循环;67℃,7分钟,以及67℃,10分钟,1次循环)进行上述的两轮聚合酶链式反应。使用Ex-Taq作为DNA聚合酶以及热循环机来完成全部的聚合酶链式反应。对所述的聚合酶链式反应产物进行测序,并且对所述的侧面基因组序列进行识别。所述的四个35S增强子被插入到5kb的基因间隔区内,所述的基因间隔区位于染色体5上的基因组AtMYB68(AT5G65790)的3’端的下游。Northern分析以及实时聚合酶链式反应表明:相对于野生型而言,在h138中引发的MYB68的表达超过野生型的2倍。
实施例3:h138突变体(myb68)的生理学特征
对植物在开花过程中的热耐受性进行评价,并且根据所述败育的花朵或者结荚的数量以及最终的种子产量进行打分。植物在受到控制的环境腔室中进行生长,其中最佳的生长条件为进行16小时200uE的光照以及8小时的黑暗,22℃以及70%的相对湿度。在所述的试验设计中使用到三组植物:1)在最佳条件下进行生长的对照组;2)进行3小时热处理的组以及3)进行4小时热处理的组。在第一次开花之后进行6天的热处理并且在1小时的时间内将所述的温度从22℃梯度上升至44℃。每组植物含有所述的myb68突变体以及所述突变体的野生型对照(myb68-空白),每个品目(entry)每种处理进行10个复制锅(replicatepots),每个锅(pot)中含有5种植物。在进行了所述的热胁迫处理的一周之后对植物的花朵败育进行评价,之后使其在最佳条件下进行生长直至成熟。对全部三组植物中的每个锅的最终的种子产量进行确定。
在经过热胁迫之后,在所述的3小时(25%)以及4小时(17%)胁迫处理组中,所述的myb68的种子产量均低于所述的myb68-空白对照,然而这种差异仅仅在所述的3小时处理组中是统计学显著的。所述的3小时处理导致所述的败育结荚相对于myb68-空白而言具有了32%的减少并且所述的最终种子产量相对于在最佳条件下进行生长的myb68植物而言增加了16%。所述的4小时处理同样导致了种子的产量相对于最佳生长的myb68植物而言具有了16%的增加。与之相反的是,所述的myb68-空白在种子的产量上相对于最佳生长的植物而言表现出15%以及23%的降低。相对于所述的野生型而言,由所述的myb68突变体提供的全部的产量保护为31%以及39%。其他的试验得出了在5%至44%的范围内的产量保护的结果,所述的结果取决于所述的试验条件。
实施例4:用于进行包括MYB 68在内的MYB-亚群14序列的表达的构建体
根据下文中所描述的方法,可以使用适宜的启动子以及本发明中所述的MYB基因制备表达载体构建体。例如由具有下述序列识别号的序列所描述的任何的基因序列,所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。这样的载体构建体能够有效的用于制备MYB68基因,与一种能够在植物细胞中发挥启动子功能的序列进行可操作性的连接并且可操作性的表达所述的基因以及由所述的基因编码的蛋白。
按照下文中的描述,在组成型启动子或者条件性启动子的常规控制下,可以对过表达MYB68的载体进行构建。所述的编码一种MYB68开放阅读框的序列与下述启动子的启动子序列进行了可操作性的连接:35S花椰菜花叶病毒(CaMV)组成型启动子,P18.2或者P81.1热诱导启动子及其内源性PMYB68启动子。除此之外,在pEGAD载体骨架中,所述的MYB68基因组序列在所述的35S花椰菜花叶病毒组成型启动子之后进行了克隆。
35S-基因组AtMYB68(存在于pEGAD载体之中)(35S-gAtMYB68)
利用聚合酶链式反应(PCR)对1.4kb的MYB68基因组DNA进行扩增,其中所述的MYB68基因组DNA中包含83bps的3’非翻译区(UTR),其中所述的扩增中使用下述引物:MYB68FW-BamH3(5’-AAAGGATCCATGGGAAGAGCACCGTGTTG-3’)(SEQ ID NO:300)以及MYB68RV-BamH4(5’-AAAGGATCCCCACTCCCTAAAGACACAGATTT-3’)(SEQ ID NO:301),并且此后利用BamHI进行消化。将上述得到的DNA片段与pBluescript II SK(+/-)进行连结,并且随后在相同的位点处在pEGAD中进行亚克隆,从而获得存在于pEGAD中的35S-基因组AtMYB68(35S-gAtMYB68)。
35S-AtMYB68,35S-AtMYB84,35S-AtMYB36,35S-AtMYB37,35S-AtMYB38,35S-AtMYB87
与AtMYB68一同,AtMYB84(At3g49690),AtMYB36(At5g57620),AtMYB37(At5g23000),拟南芥MY B38(At2g36890)以及AtMYB87(At4g37780)被划分为所述的MYB-亚群14家族中的成员(参见Stracke等人于2001年发表的文章),因此它们的功能可能是多余的。这些MYB基因在拟南芥中发生过表达,从而检验它们作为AtMYB68的直系同源基因在热耐受性方面的功能性。利用逆转录-聚合酶链式反应(RT-PCR)对所述的cDNA序列进行扩增,并且在不带有葡萄糖醛酸酶基因(GUS)的pBI121中进行克隆,从而生成35S-AtMYB84、35S-AtMYB36、35S-AtMYB37、35S-AtMYB38以及35S-AtMYB87的构建体。
通过逆转录-聚合酶链式反应(RT-PCR)制备一种1.1kb的AtMYB68cDNA,其中使用了下述引物:HG2F(5’-AAATCTAGAATGGGAAGAGCACCGTGTT-3’)(SEQ ID NO:302)以及HG2R(5’-AAAGGATCCTTACACATGATTTGGCGCAT-3’)(SEQ ID NO:303),并且利用XbaI以及BamHI进行消化。将上述得到的DNA片段在pBluescript II SK(+/-)中进行克隆,并且随后在不带有葡萄糖醛酸酶基因(GUS)的pBI121中进行克隆,从而生成35S-MYB68。不带有葡萄糖醛酸酶基因的pBI121是通过下述方法获得的:利用SmaI以及EcolcR1进行双重消化,并且随后所述的剩余载体进行自身的连结。
通过逆转录-聚合酶链式反应对AtMYB84(933bp,AtMYB84,At3g49690)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的690M84-Xba-FW上游引物(5’-acgtTCTAGAATG GGA AGA GCA CCG TGT TG-3’)(SEQ ID NO:273)以及含有一个BamHI位点的690M84-Bam-Re下游引物(5’-atcg GGATCC TTA AAA AAA TTG CTT TGA ATC AGA ATA-3’)(SEQ ID NO:274)。将所述的聚合酶链式反应产物在pBI 121的XbaI-BamHI位点处进行克隆,生成35S-AtMYB84的构建体。
通过逆转录-聚合酶链式反应对AtMYB36(1002bp,AtMYB36,At5g57620)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的M36-Xb-FW上游引物(5’-actgTCTAGAATG GGA AGA GCT CCA TGC TG-3’)(SEQ ID NO:304)以及含有一个BamHI位点的M36-Bm-Re下游引物(5’-cagt GGATCC TTA AAC ACT GTG GTA GCT CAT C-3’)(SEQ IDNO:305)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,生成35S-AtMYB36的构建体。
通过逆转录-聚合酶链式反应对AtMYB37(990bp,AtMYB37,At5g23000)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的AM37-Xb-FW上游引物(5’-actgTCTAGAATG GGA AGA GCT CCG TGT TG-3’)(SEQ ID NO:306)以及含有一个BamHI位点的AM37-Bm-Re下游引物(5’-acgt GGATC CTA GGA GTA GAA ATA GGG CAA G-3’)(SEQ IDNO:307)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,生成35S-AtMYB37的构建体。
通过逆转录-聚合酶链式反应对AtMYB38(897bp,AtMYB38,At2g36890)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的AM38-Xb-FW上游引物(5’-actgTCTAGA ATG GGT AGG GCT CCA TGT TGT-3’)(SEQ ID NO:308)以及含有一个BamHI位点的AM38-Bm-Re下游引物(5’-acgt GGATCC TCA GTA GTA CAA CAT GAA CTT ATC-3’)(SEQ IDNO:309)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,生成35S-AtMYB38的构建体。
通过逆转录-聚合酶链式反应对AtMYB87(918bp,AtMYB87,At4g37780)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的M87-Xb-FW上游引物(5’-aaaaTCTAGAATG GGA AGA GCA CCG TGC-3’)(SEQ ID NO:310)以及含有一个Bgl2位点的M87-Bg-Re下游引物(5’-aaaa AGATCTCTA CTC ATT ATC GTA TAG AGG-3’)(SEQ ID NO:311)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,生成35S-AtMYB87的构建体。
P18.2-MYB68,以及P81.1-MYB68
所述的构建包括四个步骤:1)通过聚合酶链式反应对869bp的热休克蛋白(Hsp)18.2启动子以及406bp的热休克蛋白(Hsp)81.1启动子进行扩增,其中分别使用到下述引物组:HP1F(SEQ ID NO:277)与HP1R(SEQ ID NO:278),以及HP2F(SEQ ID NO:279)与HP2R(SEQID NO:280),并且利用SalI以及XbaI进行消化。将上述得到的DNA片段在相同的位点处在pBI101中进行克隆,从而生成所述的新的载体:P18.2pBI101以及P81.1pBI101;2)在XbaI位点以及SmaI位点处,在所述的新的载体中进行MCS2-寡核苷酸(MCS2-oligo)(包括下述限制性位点:XbaI,HpaI,AgeI,KpnI,XhoI,ScaI,SpeI,SalI,BamHI以及SmaI)的克隆。将上述得到的载体命名为P18.2pBI101MCS以及P81.1pBI101MCS;3)通过利用SmaI以及EcolcR1的双重消化以及随后的剩余载体的自身连结,除去所述的葡萄糖醛酸酶(GUS)基因,从而获得不带有葡萄糖醛酸酶基因(GUS)的P18.2pBI101MCS载体以及不带有葡萄糖醛酸酶基因(GUS)的P81.1pBI101MCS载体;4)在XbaI位点以及BamHI位点处,将所述的1.1kb的MYB68cDNA片段连结在所述的两个最新的载体之中,从而为P18.2-MYB68完成了不带有葡萄糖醛酸酶基因的P18.2pBI101MCS的构建,并且为P81.1-MYB68完成了不带有葡萄糖醛酸酶基因的P81.1MCSpBI12的构建。pHSP81.1-AtMYB68
利用XbaI以及BamHI进行限制性消化来分离所述的AtMYB68的编码序列,并且将其在pHSP81.1的XbaI-BamHI位点处进行克隆,其中所述的XbaI以及BamHI来自于质粒pHSP18.2-AtMYB68。
pHPR-AtMYB68
利用聚合酶链式反应从拟南芥基因组DNA中对所述的拟南芥羟基丙酮酸还原酶基因(HPR,Atlg68010)的启动子序列(-1至-506bp,相对于ATG起始密码子而言)进行扩增,其中所述的扩增使用到下述引物:含有一个Sal I位点的上游引物(HPR-Sal-FW,acgt gtcgacGAAGCAGCAGAAGCCTTGAT)(SEQID NO:312)以及含有一个Xba I位点的下游引物(HPR-Xb-R2,acgt tctaga GGT AGA GAA AAG AGA aag cct c)(SEQID NO:313)。将上述经过消化的片段在所述的载体pHSP81.1-AtMYB68中进行克隆,其中所述的pHSP81.1-AtMYB68载体利用SalI以及XbaI进行了预先消化,从而除去了所述的热休克蛋白(HSP)81.1启动子。这样生成了一个重组质粒,其中所述的羟基丙酮酸还原酶基因(HPR)启动子位于AtMYB68的位置之前。
PMYB68-AtMYB68
通过聚合酶链式反应对所述的AtMYB68启动子(从-1至-1034,相对于所述的MYB68ATG起始密码子而言)进行扩增,所述的扩增中使用下述引物:Pm-68-H3-FW(SEQ IDNO:275)以及Pm68-Av-Xh-Re(SEQ ID NO:276),并且利用下述限制酶进行消化:HindIII以及XhoI。将上述得到的启动子片段在相同的位点处在不带有葡萄糖醛酸酶基因的P81.1MCSpBI121中进行克隆,从而替代所述的热休克蛋白(Hsp)81.1启动子。之后将这种载体命名为PMYB68pBI121,并且将其用于在AvrII位点以及BamHI位点处进行AtMYB68cDNA(1.1kb)的进一步克隆,从而获得PMYB68-AtMYB68。从所述的质粒18.2-MYB68中回收所述的MYB cDNA的AvrII-BamHI片段。
pM68-AtMYB84
通过逆转录-聚合酶链式反应对AtMYB84(933bp,AtMYB84,At3g49690)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的690M84-Xba-FW上游引物(5’-acgtTCTAGA ATG GGA AGA GCA CCG TGT TG-3’)(SEQ ID NO:273)以及含有一个BamHI位点的690M84-Bam-Re下游引物(5’-atcg GGATCC TTA AAA AAA TTG CTT TGA ATC AGA ATA-3’)(SEQ ID NO:274)。将所述的聚合酶链式反应产物在pB-Pm68的AvRII-BamHI位点处进行克隆,在所述的AtMYB68启动子的控制下生成AtMYB84的构建体。
pM68-AtMYB36
通过逆转录-聚合酶链式反应从年幼的拟南芥幼苗(叶片以及根部)中分离出的RNA中对AtMYB36(1002bp,At5g57620)的编码序列进行扩增,其中使用到下述引物:含有一个XbaI位点的M36-Xb-FW上游引物(5’-actg TCTAGA ATG GGA AGAGCT CCA TGC TG-3’)(SEQ ID NO:305)以及含有一个BamHI位点的M36-Bm-Re下游引物(5’-cagt GGATCC TTAAACACT GTG GTA GCT CAT C-3’)(SEQ ID NO:306)。将所述的聚合酶链式反应产物在如上所述的pBI-Pm68的AvRII位点以及BamHI位点处进行克隆。这样一来,在所述的AtMYB68启动子的控制下生成了AtMYB36的构建体。
35S-水稻MYB36(35S-OsMYB36)
水稻MYB36cDNA同系物:利用逆转录-聚合酶链式反应从水稻根部RNA中对水稻MYB36基因的编码序列(966bp)进行扩增,其中所述的水稻MYB36基因的编码序列编码一种被识别为序列识别号10的蛋白,在所述的扩增中使用了下述引物:含有一个XbaI位点的rM-Xb-FW2上游引物(5’-acgt TCTAGAATG GGG AGA GCG CCG TGC TG-3’)(SEQ ID NO:314)以及含有一个BamH I位点的rM-Bm-Re2下游引物(5’-tgca GGATCC CTA CTG CAT CCC GAGGTC AG CT-3’)(SEQ IDNO:315)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,从而生成35S-水稻MYB36的构建体。
35S-基因组水稻MYB36(35S-gOsMYB36)
水稻MYB基因组同系物克隆:利用与如上所述的相同的引物:rM-Xb-FW2上游引物(SEQ ID NO:314)以及rM-Bm-Re2下游引物(SEQ ID NO:315),对所述的水稻MYB36基因的基因组序列(SEQ ID NO:265)进行扩增(1259bp)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,从而生成35S-基因组水稻MYB36的构建体。
35S-大豆MYB84(35S-GmMYB84)
所述的大豆MYB161是AtMYB84的同系物。这里所述的术语“大豆MYB84”可以与大豆MYB161进行互换使用。利用逆转录-聚合酶链式反应从大豆根部RNA中进行所述的1068bp的大豆MYB161编码序列的克隆,其中使用到下述引物:含有一个XbaI位点的soybM-Xba-FW2上游引物(5’-acgt TCTAGA ATGGGG AGG GCA CCT TGC T-3’)(SEQ ID NO:316)以及含有一个BamHI位点的soybM-Bm-Re下游引物(5’-acgt GGATCCTA TTG CGC CCC CGG GTA G-3’)(SEQID NO:317)。将所述的聚合酶链式反应产物在pBI121的XbaI-BamHI位点处进行克隆,从而生成35S-大豆MYB84的构建体。
35S-玉米MYB36(35S-ZmMYB36)
利用聚合酶链式反应对所述的玉米MYB36cDNA(SEQ IDNO:261)进行扩增,其中使用了下述引物:ZmYYBFW-XbaI(5’-aaatctagaATGGGGAGAGCTCCGTGCTGCGACA-3’)(SEQ IDNO:318)以及ZmMYBRV-BamHI2(5’-aaaggatccCTACTTCATCCCAAGGTTTCCTGGC-3’)(SEQ IDNO:319)。利用XbaI以及BamHI对所述的DNA片段进行消化并且随后在同样的位点处与pBluescript II SK(+/-)进行连结,并且之后在所述的pBI121的相同位点处进行亚克隆,从而替代葡萄糖醛酸酶基因(GUS)。
35S-棉花MYB68(35S-GhMYB68或者35S-CotMYB68)
利用聚合酶链式反应对所述的棉花MYB68cDNA进行扩增,其中使用了下述引物:CotM-Xb-Fw(5’-acgt TCTAGA ATG GGG AGA GCT CCT TGT TG-3’)(SEQ ID NO:320)以及CotM-BmRe(5’-acgt GGATCC CTA TTG CGC TCC TCCTGG G-3’)(SEQ ID NO:321)。利用XbaI以及BamHI对所述的DNA片段进行消化。将其在同样的位点处与pBluescript II SK(+)进行连结,并且之后在所述的pBI121的相同位点处进行亚克隆,从而替代葡萄糖醛酸酶基因(GUS)。
35S-油菜MYB68r(35S-BnMYB68r)
利用聚合酶链式反应对所述的油菜根部MYB68cDNA进行扩增,其中使用了下述引物:Bn68root-Fw-XbaI(5’-aaatctagaATGGGAAGAGCACCGTGTTGTGATAAGGCC-3’)(SEQ ID NO:322)以及Bn68root-RV-BamHI(5’-aaaggatcc TTACACATTATTTGGCCCATTGAAGTATCTTGC-3’)(SEQ ID NO:323)。利用XbaI以及BamHI对所述的DNA片段进行消化。将其在同样的位点处与pBluescript II SK(+)进行连结。之后将上述片段在所述的pBI121的相同位点处进行亚克隆,从而替代葡萄糖醛酸酶基因(GUS)。
35S-油菜MYB68b(35S-BnMYB68b)
利用聚合酶链式反应对所述的油菜蓓蕾的MYB68cDNA进行扩增,其中使用了下述引物:Bn68Bud-Fw-XbaI(5’-aaatctagaATGGGAAGAGCACCGTGTTGTGACAAGGCT-3’)(SEQ IDNO:324)以及Bn68Bud-RV-BamHI(5’-aaaggatcc TTACAAATGATTTGCCCCATTGAAGTAACTTGC-3’)(SEQ ID NO:325)。利用XbaI以及BamHI对所述的DNA片段进行消化。将其在同样的位点处与pBluescript II SK(+)进行连结。之后将上述片段在所述的pBI121的相同位点处进行亚克隆,从而替代葡萄糖醛酸酶基因(GUS)。
下面的表格2描述的是在制备如上所述的载体构建体中所使用到的寡核苷酸引物,以及能够有效的用于克隆AtMYB同系物的其他的引物。
表格2:用于进行AtMYB68同系物的克隆而合成的寡核苷酸引物
序列识别号 引物名称 限制性位点 序列(5’-3’) 备注
271 790M68-Xba-FW Xba I ACGT TCTAGA ATG GGA AGA GCA CCG TGT TG AtMYB68(at5g65790)
272 790M68-Bam-Re BamH I ATCG GGATCC TTACAC ATG ATTTGG CGC ATT G AtMYB68(at5g65790)
273 690M84-Xba-Fw Xba I ACGT TCTAGA ATG GGA AGA GCA CCG TGT TG AtMYB84(at3g49690)
274 690M84-Bam-Re Bam H I ATCG GGATCC TTA AAA AAA TTG CTT TGA ATC AGA ATA AtMYB84(at3g49690)
275 Pm68-H3-FW Hind III ACGT AAGCTT TCG TAA AAT CTC TCA TG AtMYB68启动子
276 Pm68-Av-Xh-Re Av r II以及Xho I GTCA CTCGAG CCTAGG TTT CTT GAT TCT TGA TTC TTG ATC AtMYB68启动子
277 HP1f AAAGTCGACGCATCTTTACAATGTAAAGCTTTTCT
278 HP1R AAATCTAGATGTTCGTTGCTTTTCGGG
279 HP2F AAAGTCGACAGAAGACAAATGAGAGTTGGTTTATATTT
280 HP2R AAATCTAGACGCAACGAACTTTGATTCAA
281 BnMYB68FW2 ATGGGAAGAGCACCGTGTTGTGATAAGGCC 油菜MYB68(AC189266.1)
282 BnMYB68RV2 TTAATTTGGCGCATTGAAGTAACTTGCATCTTCGG 油菜MYB68(AC189266.1)
283 rM-Xb-Fw Xba I ACGT TCTAGA ATG GGG AGA GCG CCG TGC 水稻MYB(AAT85046)
284 rM-Bm-Re Bam H I TGCA GGATC CTA CTG CAT CCCGAG GTC AG 水稻MYB(AAT85046)
285 cotM-Xb-Fw Xba I ACGT TCTAGA ATG GGG AGA GCT CCT TGT TG 棉花MYB(TC34239)
286 cotM-Bm-Re Bam H I ACGT GGATCC CTA TTG CGC TCC TCC TGG G
287 soybM-Xba-FW2 Xba I ACGT TCTAGA ATG GGG AGG GCA CCT TGC T 大豆MYB(ABH02906)
288 cornM-Xba-FW2 Xba I I ACGT TCTAGA ATG GGG AGA GCT CCG TGC T 玉米MYB(TC370133)
289 wheatM-Xba-FW Xba I ACTG TCTAGA ATG GGG AGG GCG CCG TGC 小麦MYB(BQ483726)
290 MedtM-Xba-Fw Xba I ACTG TCTAGA ATG GGA AGA GCT CCT TGC TGT 截形苜蓿MYB(TC97441)
291 sorgM-Xb-FW Xba I ACGTTCTAGAATGGGGAGAGCTCCGTGCT 高粱MYB(AAL84760)
292 toM-Xb-FW Xba I ACTGTCTAGAATGGGAAGAGCTCCATGTTGT 番茄blind(AAL69334)
293 toM-Bm-Re Bam H I GACT GGATCC TTA GTA ATA AAA CAT CCC ATA CTC A
294 popM-Xb-FW Xba I ACGT TCTAGA ATG GGG AGA GCT CCT TGC TG 白杨MYB(TC54478)
295 popM-Bm-Re Bam H I GACT GGATCC TCA TTG TGG CCC AAA GAA GCT 白杨MYB(TC54478)
296 HSP18.2 HP1F AAAGTCGACGCATCTTTACAATGTAAAGCTTTTCT
297 HSP18.2 HP1R AAATCTAGATGTTCGTTGCTTTTCGGG
298 HSP81.1 HP2F AAAGTCGACAGAAGACAAATGAGAGTTGGTTTATATTT
299 HSP81.1 HP2R AAATCTAGACGCAACGAACTTTGATTCAA
注释
1.FW:带有基因特异性序列的上游引物,其中所述的基因特异性序列是从所述的ATG起始密码子开始的。
2.Re:带有基因特异性序列的下游引物,其中所述的基因特异性序列是从所述的终止密码子开始的。
3.用下划线表示在所述的5’端的限制性位点。
可以将本发明所述的表达载体构建体导入到拟南芥、所述的起源植物或者所选择的任何物种之中。例如一个AtMYB基因可以在芸薹属物种中进行过表达,或者可以选择的在大豆、玉米、水稻或者棉花物种中进行过表达。
实施例5:MY B68的氨基酸序列分析
下面的表格提供了对于来自于不同的植物物种的MYB基因家族的氨基酸序列的比较,其中为进行多重序列的比对以及氨基酸序列的分析而进行了不同的设置。注释:在针对不同的植物物种的所述文献以及数据库中使用不同的MYB命名以及编号体系。
在下面的表格中,(*)表示根据肽序列的比对对所述预测的开放阅读框(ORF)基因组DNA序列进行编辑,从而生成一个推定的编码序列序列。所述的(P)标识表示的是一个部分的序列。利用ClustalW在http://www.ebi.ac.uk/clustalw/.上对序列的同源性以及多重序列的比对进行比较。
表格3:
表格4:来自于各种不同作物/物种的AtMYB68同系物
在上述的表格4当中,利用ClustalW在http://www.ebi.ac.uk/clustalw/上对序列的同源性以及多重序列的比对进行比较,其中使用到下述的参数设定:
矩阵(Matrix):Gonnet 250
开放的缺口(GAP OPEN):10.0
末端缺口(END GAPS):-1
缺口的延伸(GAP EXTENSION):0.2
缺口的距离(GAP DISTANCES):4
表格5:来自于各种不同作物/物种的AtMYB68同系物
在上述的表格5当中,利用ClustalW在http://www.ebi.ac.uk/clustalw/上对序列的同源性以及多重序列的比对进行比较,其中使用到下述的参数设定:
矩阵(Matrix):蛋白:Gonnet 250
开放的缺口(GAP OPEN):DNA:15.0蛋白:10.0
末端缺口(END GAPS):-1
缺口的延伸(GAP EXTENSION):DNA:6.66 蛋白:0.2
缺口的距离(GAP DISTANCES):4
实施例6:35S-MYB68表达品系的生理学特征
在一个转基因品系群体中,将存在一种表达水平以及生理学应答的分级。在某种程度上,所述的变化的分级是所述的基因构建体的整合位点以及在这一位点上进行的基因表达的局部环境的结果。因此,为了筛选出具有最佳表现的品系,必须对品系进行筛选以及评价。这种方法对于本领域技术人员而言是一种常规的方法。
在一项热与花朵败育试验中,对表达一种35S-MYB68表达构建体的纯合品系进行了评价。所述的试验方案包括每个品系的8个复制植物,每个3尺的锅(3’pot)中含有1个植物,并且使其在一个受到控制的环境腔室(18小时200uE的光照,6小时的黑暗,22℃,70%的相对湿度)中在最佳条件下进行生长。在最初的开花出现之后的三天,将植物在42℃的热休克条件下暴露1小时,并且将其送回至最佳条件下进一步生长7天。对植物的主茎上的花朵败育情况进行评价。对品系进行识别,其中被识别出的品系与野生型对照相比,其表现出的花朵败育率减少了34%至60%。
对九种独立的35S-MYB68转基因品系的种子产量以及产量保护进行确定,其中使用相对于野生型的百分数进行表达。所述的试验方案在每个3尺的锅(pot)中包括3个植物,所述的植物在如上所述的条件下进行生长,每个品系含有22个复制体。在最初的开花出现之后的三天,将每个品系中的12个复制体在45℃下的热胁迫条件下暴露3小时,其中所述的温度是在1小时的时间里由22℃梯度上升为45℃的。三天之后,将上述的热胁迫再施加一次。使每个品系中的剩余10个复制体植物在它们的全部生命周期中被维持在最佳条件下进行生长。对于上述全部植物的最终种子产量进行确定。由于上述施加的热胁迫,野生型植物在产量上表现出40%的下降,而转基因品系由于热胁迫的原因尽管也具有下降的产量,但所述的下降程度较轻,形成了10%至12%的产量保护。
对筛选出的品系进行再次评价并且按照下述方式进行胁迫。将植物暴露于1小时的温度梯度上升阶段,从22℃上升至45℃,并且使45℃的热胁迫保持1.5至1.8小时。在连续的五天时间里每天施加热胁迫,在此之后进行五天的恢复阶段并且之后进行第六次的热处理。上述的热处理导致野生型(WT)植物在产量上发生了11%的下降,而在四种转基因品系中观察到在2%至17%范围内的产量的增加。正如表格6中所示,在接受了所述的热胁迫之后,相对于野生型而言,六种转基因品系(68,80,93,73,83,30)表现出了产量保护。这种保护在3%至31%的范围内。这些转基因品系中的两种(30以及73)已经在先前的试验中表现出了产量的保护,而两种品系(93以及83)表现出了花朵败育的减少。
表格6:
品系 热种子产量 相对于野生型而言的产量保护
68 0.816 31%
80 0.714 26%
93 0.781 17%
73 0.820 14%
83 0.719 5%
30 0.801 3%
野生型 0.784 -
myb68 0.577 15%
野生型(myb68-空白) 0.779 -
表达所述的PMYB68-AtMYB68构建体的拟南芥(Arabidopsis)品系的特征
在一项花朵败育试验中,对T3世代的十四种纯合的转基因品系进行了评价。使植物(每个品目针对十四个复制体)在2.25尺的锅(pots)中在最佳条件下(18小时的光照,200uE,22℃,60%的相对湿度)进行生长,直至距离第一次开花有三天的时间。在这一时间点处将植物暴露于43℃下进行1小时的处理。在所述的热处理过后,将植物送回至最佳条件下继续进行7天的生长。在这一时间点处,通过对存在于被暴露于上述的热胁迫中的所述区域内的败育结荚(pod)以及被损伤的(变短的)结荚(pod)进行计算,从而对上述的热处理对于结荚的形成所产生的影响进行评价。与对照组(野生型-哥伦比亚以及所述的空白-n11-8)相比,六种转基因品系在被损伤的结荚的总数量上表现出了减少(参见下面的表格7)。所述最佳的品系仅仅表现出了对照组的热损伤的70%。在这里进行检查的两种转基因品系在所述的T2阶段的筛选中同样表现出了减少的花朵败育。所述的被作为一种阳性对照而包括在内的myb68突变体同样在被损伤的结荚总数量上表现出了减少,这种减少是与所述的myb68突变体的分离的空白对照(myb68-空白)相比较而言的。
表格7:
实施例7:对表达MYB68的转基因植物进行的生理学评价
AtMYB68突变体表现出干旱耐受性
使每个3尺的锅(pot)中的五个植物在生长腔室中在16小时的光照(180uE)、22℃、70%的相对湿度(RH)的最佳条件下进行生长,直至第一次开花,其中每个品目中针对6个复制体。开始进行干旱处理,对所有锅中的水的量进行平衡并且停止浇水。通过对所述的锅进行称重从而每天测量水分的损失。对土壤的水分含量(SWC)进行计算,用初始的%来表示。在所述的干旱处理的第0天、第2天以及第4天对植物进行收集。对相对于所述植物的嫩枝干重而言的水分损失进行计算。在经历了所述的干旱处理之后,所述的myb68植物比对照组表现出了具有更高的土壤水分含量的趋势。嫩枝的重量没有差别或者比对照组的重量稍重一些。与对照组相比,所述的myb68突变体具有的两天里的水分损失与第二天的嫩枝干重的比例较低,这表明其具有干旱耐受性的趋势。
表格8:在干旱处理的时间里的土壤水分含量(初始的%)以及相对于第二天的嫩枝干重而言在两天时间里的水分损失
myb68突变体以及35S-基因组MYB68以及35S-MYB68拟南芥转基因品系在出苗阶段表现出对于盐的耐受性
将种子进行灭菌并且将其放置于琼脂平板之上,在所述的琼脂平板上带有1/2的MS生长培养基,所述的MS生长培养基中含有盐(200毫摩氯化钠)或者不含盐(最佳平板),每个品目(entry)中具有6个平板并且每个平板上具有30个种子。在4℃下放置3天之后,将平板放置于所述的生长室中,在22℃、以及18小时的光照(100uE)下生长16天。经过7天之后,对平板中的萌芽状况进行打分,并且再经过9天之后对漂白的出苗状况进行打分(表示胁迫的白色幼苗%)。在最佳平板以及盐平板上,没有发现对照组以及转基因品系或者所述的突变体在萌芽状况中存在差别。但是在被暴露于盐中16天之后,幼苗通过被漂白而表现出胁迫的迹象。结果表明所述的myb68突变体以及所述的转基因35S-MYB68表达品系具有更少的漂白苗,这代表着对于盐胁迫具有较低的敏感性。
表格9:在含有琼脂的200毫摩氯化钠以及1/2MS平板中进行16天的培养之后对被漂白的幼苗的打分(白色幼苗%)
MYB68在拟南芥中的组成型表达引发了热胁迫之后的产量保护
使所述的35S-Myb68拟南芥植物在3尺的锅(pot)中进行生长,每个锅中装有3个植物,并且每次最佳处理是针对10个复制体进行的,而每次热处理是针对12个复制体进行的。让所有的植物在最佳条件下进行生长直至开花进行两天。在这一时间点上使最佳植物仍然保留在最佳条件下(22℃,18小时200uE的光照,70%的相对湿度)并且每天向所述的测试组施加一次热处理,其中所述的热处理是:在1小时的梯度时间里将温度由22℃升高至45℃并且将这一温度保持1.5至2.5小时,所述的热处理连续进行五天。使植物恢复两天的时间,在此期间不对其施加胁迫,在此之后再次施加三天的胁迫(共计八天的热处理)。在所述的热处理之后,将植物与所述的最佳组一同维持在最佳条件下直至成熟,并且对两个组的最终种子产量进行确定。所述的结果表明,四种35S-Myb68转基因品系(22-7,20-11,35-1以及8-6)在经过了所述的热胁迫处理之后表现出产量的保护,相对于对照组而言,所述的产量保护在8%至21%的范围之内。
表格10:每个锅(pot)中的种子产量,所述的种子产量来自于如上所述的在最佳条件下生长的植物以及在热胁迫条件下生长的植物。通过在经过所述的热胁迫之后所述的种子产量间的差异计算出产量保护,并且将其表示为转基因品系以及对照组中的最佳情形的百分数(%)。
实施例8:对AtMYB-亚群14序列及其来自于其他物种的同系物所具有的制备所述 的目标表现型例如热耐受性表现型的功能的确认
油菜MYB68在拟南芥中的组成型表达引发了热胁迫之后的减少的花朵败育
从芸薹属(Brassica)中识别出两个高度相关的Myb68序列。它们的表达类型的不同之处在于:其中一个主要在所述的根部中进行表达(SEQ ID NO:54),另外一个主要在花蕾中进行表达(SEQ ID NO:56)。对含有能够表达任意的所述油菜Myb68的构建体的植物进行生产以及评价。使植物在2.25尺的锅(每锅一个植物)中、在最佳条件下(22℃,50%的相对湿度,17小时200uE的光照)进行生长,直至距离第一次开花三天的时间。将植物从22℃下转移至43℃下并保持2小时-2.5小时(参见下面的表格)。在经过这种热胁迫之后,将植物送回至22℃的最佳条件下保持一周。在经过所述的热胁迫一周之后,对败育花朵的数量进行计算。与对照组(空白)相比,35S-油菜Myb68(根)构建体的转基因品系表现出较少的败育蓓蕾。在经过热胁迫之后,与对照组(空白品系)相比,两个35S-油菜Myb68(蓓蕾)构建体的转基因品系表现出减少的花朵败育。
表格11:在经历过43℃下的2小时热胁迫之后,败育花朵的数量——35S-油菜MYB68(根)
表格12:在经历过43℃下的2.5小时热胁迫之后,败育花朵的数量——35S-油菜MYB68(蓓蕾)
大豆MYB84或者AtMYB84或者AtMYB36在拟南芥中的组成型表达引发了热胁迫之后的减少的花朵败育
制备大豆-Myb84(GmMyb84)或者AtMYB84(AtMyb84)或者AtMYB36(AtMyb36)的过表达构建体,并且将所述的过表达构建体转化至拟南芥植物中,已经对所述的Myb-亚群14同系物产生热耐受性的功能进行了证实,所述的功能是通过在热胁迫下发生的减少的花朵败育来证明的。制备转基因植物并且所述的T2世代被用来进行热耐受性的初始筛选。随后,T3纯合转基因植物被用来进行详细的生理学评价以及对初始结果的证实。
将所述的T2种子放置于0.5倍的MS琼脂平板上,所述的平板上含有维生素(每个平板上一层)。每个测试组中包括一个阳性对照,所述的初始的热耐受性突变体myb68,以及一个相应的野生型。在萌芽之后的第十天将幼苗移植到土壤中。在早期的开花阶段,将植物放置于一个45℃下的加热腔室中(湿度65%),保持24-30分钟。之后将所述的植物放回到所述的具有正常的生长条件(17小时的光照/7小时的黑暗,200uE,22℃,湿度70%)的生长室中。在第三十二天对植物进行检查并且对败育的长角(silique)、部分败育的长角、坏死的分生组织或者正常的长角进行打分。如果绝大部分的野生型植物出现了显著的花朵败育,则表明热胁迫确实发挥了作用。利用逆转录-聚合酶链式反应对转基因品系的基因表达进行评价并且证明其具有提升的表达水平。
使用Myb-亚群14序列的同系物制备构建体以及转基因植物,并且所述的构建体以及转基因植物提升了热耐受性,其中所述的Myb-亚群14序列的同系物来自于目标作物物种,例如,水稻,玉米,小麦,大豆以及棉花。
表格13:
构建体 作为%对照的花朵败育
35S-油菜MYB68-蓓蕾 84
35S-油菜MYB68-根 82
35S-玉米MYB84 58
35S-AtMYB84 77
35S-AtMYB36 81
这个实施例证明,拟南芥可以被用来作为一种模型系统,用来评价以及提供构象,Myb-亚群14序列能够提供热耐受性并且从其他的植物物种中识别出来的这一序列能够在拟南芥中发挥功效。
表达35S-AtMYB36构建体的拟南芥品系的特征
在一项花朵败育试验中,对T3世代的十五种纯合的转基因品系进行了评价。使植物(每个品目针对十四个复制体)在2.25尺的锅(pots)中在最佳条件下(18小时的光照,200uE,22℃,60%的相对湿度)进行生长,直至距离第一次开花有四天的时间。在这一时间点处将植物暴露于43℃下进行1小时的处理。在所述的热处理过后,将植物送回至最佳条件下继续进行7天的生长。在这一时间点处,通过对存在于被暴露于上述的热胁迫中的所述区域内的败育结荚(pod)以及被损伤的(变短的)结荚(pod)进行计算,从而对上述的热处理对于结荚的形成所产生的影响进行评价。与对照组(野生型-哥伦比亚以及所述的空白-n77-4)相比,九种转基因品系在被损伤的结荚的总数量上表现出了减少(参见下面的表格7)。在这里进行检查的三种品系在所述的T2阶段的筛选中同样表现出了减少的花朵败育。所述的被作为一种阳性对照而包括在内的myb68突变体同样在被损伤的结荚总数量上表现出了减少,这种减少是与所述的myb68突变体的分离的空白对照(myb68-空白)相比较而言的。
表格14:
AtMYB68在油菜(Brassica napus)中的组成型表达或者诱导型表达表现出了热胁迫之后的减少的花朵败育
在生长腔室的环境下,对于含有AtMYB68基因序列的五种转基因品系的热胁迫耐受性进行评价,其中所述的转基因品系受到了组成型启动子或者热诱导型启动子的控制。这些品系处于所述的T2阶段并且是杂合的,除了所述的01-105G-1-E品系之外。相比较于在纯合品系中完成的分析而言,对于杂合品系进行的同样的分析通常会产生更大的变化。然而,早期的分析允许对所述的衍生的纯合品系进行筛选以及随后的分析。分离的空白以及所述的母本DH12075作为对照被包括在内。所述的试验被编排为一种裂区设计,其中利用温度作为主要因素并且利用转基因品系作为次要因素。使所述的植物在15厘米的塑料锅中进行生长,在所述的塑料锅中装有“Sunshine Mix#3”,所述的植物接受到大约500微摩/平方米秒的光合有效辐射,所述的辐射作用于所述的作物冠层的顶端。进行分子分析从而确认转基因的存在。在所述的测试中包括两组植物:一组在其全部的生长时期内均在最佳条件(22℃/18℃的日/夜温度,16小时的光周期)下进行生长,而所述的第二组每天经受5小时的31℃下的热胁迫(从18℃梯度上升至31℃,之后下降回18℃)。在所述的第一次开花之后的第三天开始进行热胁迫条件的处理并且在此之后持续七天的时间。之后将所述的热胁迫植物送回至最佳条件下直至其成熟。在所述的热胁迫周期开始的时候以及结束的时候对所述的胁迫植物的所有的总状花序进行标记,用以指明哪些花朵经受了热胁迫。对于存在于所述被标记的总状花序之上的存活的结荚以及败育的结荚进行计算,并且通过热胁迫条件下败育的结荚与败育的结荚加上存活的结荚的总数的比值来计算所述的结荚败育率,以百分数来表示。在收获之后确定种子的产量。
在这些不同的品系之间,在花朵败育上存在着显著的差异,与所述的母本品系相比,两种35S-Myb68品系在经过热胁迫之后表现出显著降低的败育率。与其分离的空白(48%)以及DH12075(61%)相比,品系02-104G-4-A具有显著降低的败育率(33%)。品系02-104G-3-K的败育率(47%)同样显著的低于所述的分离的空白(66%)以及DH12075的败育率。与其分离的空白(58%)以及所述的母本对照相比,所述的纯合品系(01-105G-1-E)具有些微降低的败育率(53%)。在一个转基因品系群体中,将存在一种表达水平以及生理学应答的分级。在某种程度上,所述的变化的分级是所述的基因构建体的整合位点以及在这一位点上进行的基因表达的局部环境的结果。这种分级是可以预期的并且因此,为了筛选出具有最佳表现的品系,必须对品系进行筛选以及评价。这种方法对于本领域技术人员而言是一种常规的方法。
在上述的受试品系间存在着种子产量的差异。所述的三种转基因品系02-104G-3-K、02-104G-4-A以及01-105G-1-E的产量与所述的母本品系的产量相似,然而与其自身的空白(null)相比,所述的三种品系在种子产量上表现出了10%至22%的增长。与所述的DH12075母本相比,两种转基因品系(02-33H-1-V以及01-105G-3-G)呈现出的表现不佳,然而与适宜的空白(null)相比,一种品系则显著的较低。由于这些品系的接合性,这样的差异度是未预料到的。
在存在于热胁迫条件下的每个总状花序的种子的数量方面发现了类似的趋势。与所述的母本品系相比,品系02-104G-3-K、02-104G-4-A以及01-105G-1-E在每个总状花序上具有些许增加的种子数量,但是其他的转基因品系(02-33H-1-V以及01-105G-3-G)在每个总状花序上具有显著减少的种子数量。在上述的受试品系间,在100粒种子的重量上不存在显著的差异。
概括的说,在开花期间施加的热胁迫过程中,表达所述的AtMYB68基因的两种转基因品系证明了其对于花朵败育产生了显著的保护。除此之外,三种转基因品系表现出了一种种子产量增加的趋势。
表格15:
构建体 品系 败育率% 标准误差 败育%空白 产量 标准误差 产量%空白
35S-MYB68 02-104G-3-K 47 4.8 71 0.71 0.188 122
02-104G-3G空白 66 3.7 0.58 0.091
02-104G-4-A 33 7.6 69 0.75 0.153 110
02-104G-4-F空白 48 2.8 0.68 0.090
02-33H-1-V 79 1.9 116 0.31 0.159 44
02-33H-1-F空白 68 4.3 0.71 0.090
P18.2-MYB68 01-105G-1-E 53 4.1 91 0.73 0.097 116
01-105G-1-B空白 58 3.0 0.63 0.090
01-105G-3-G 75 4.9 94 0.31 0.099 96
01-105G-3-J空白 80 3.6 0.32 0.097
对照 DH12075母本 61 2.4 0.62 0.106
实施例9:MYB-亚群14序列及其同系物的识别
识别AtMYB序列的方法、将MYB序列划分成指定的亚群的方法以及识别MYB-亚群14序列的方法在Stracke等人于2001年发表的文章中以及在Kranz等人于1998年发表的文章中进行了进一步的描述。MYB-亚群14序列已经被定义为一种核苷酸序列或者一种蛋白序列,其中包括拟南芥,这样的特征在本发明中也有所描述。所述的MYB-亚群14是一种R2R3MYB序列,所述的序列额外包括一种保守的基序,所述的基序是通过下述的形式来进行描述的。
所述的一般模式(SEQ ID NO:266)提供了一种序列,所述的序列可以被用来识别一种备选的MYB-亚群14序列。在某些位置处,对于所述给定的位置而言,允许存在多个氨基酸残基。在允许存在多个氨基酸的情况下,将所述任选的残基在方括号内进行表示。当一种R2R3MYB蛋白序列能够与所述的一般模式相互匹配(fit)时,所述的蛋白序列可能是一个MYB-亚群14序列。一个MYB-亚群14序列可能不完全等同于所述的一般模式,例如它可以是90%、95%或者99%等同的。
如果一个备选的MYB-亚群14序列与所述的一般模式相互匹配,并且其进一步的包括一种与所述的排他模式(SEQ ID NO:267)之间的匹配,那么所述的序列是一种被包括在内的有力的备选者,成为一个MYB-亚群14序列。所述的排他序列(SEQ ID NO:267)定义了氨基酸的类型,这些氨基酸存在于MYB-亚群14序列中但是可能与其他的R2R3MYB蛋白产生不同。所述的排他模式在一种MYB蛋白中的出现是一种有力的指示,表明所述的MYB是所述的MYB-亚群14家族中的一个成员。
如果一个备选的MYB-亚群14序列与所述的一般模式相互匹配,并且其进一步的包括一种与所述的绝对模式(SEQ ID NO:268)之间的匹配,那么所述的序列是一种被包括在内的有力的备选者,成为一个MYB-亚群14序列。所述的绝对模式(SEQ ID NO:268)代表的是到目前为止分析出的存在于所有的MYB-亚群14序列中的序列残基。
对于下面列出的所述的一般模式、排他模式、以及绝对模式(SEQ ID NO:266-268)而言,“X”表示的是任意的氨基酸,“X(N)”,其中N表示任意数字,表示的是由所指示的数量个“X”构成的一串(即,X(23)表示的是由23个“X”构成的一串),其中X是任意的氨基酸。在某些位置处,对于所述给定的位置而言,允许存在多个氨基酸残基。在允许存在多个氨基酸的情况下,将所述任选的残基在方括号内进行表示。
一般模式(SEQ ID NO:266)
M-G-R-X-P-C-C-D-[KR]-X-X-[MV]-K-[RK]-G-X-W-[SA]-X-[DQE]-E-D-X-X-[IL]-[RK]-X-[FY]-X-X-X-X-G-X-X-X-[SN]-W-I-X-X-P-X-[RK]-X-G-[IL]-X-R-C-G-[KR]-S-C-R-L-R-W-[IL]-N-Y-L-R-P-X-[IL]-[RK]-H-G-X-[FY]-[ST]-X-X-E-[DE]-X-X-[IV]-X-X-X-[FY]-X-X-X-G-S-[KR]-W-S-X-[MI]-A-X-X-[ML]-X-X-R-T-D-N-D-[ILV]-K-N-[HY]-W-[DN]-[ST]-[RK]-L-[RK]-[RK]-[RK]
排他模式(SEQ ID NO:267)
[RK]-X(9)G-X-X-I-X(28)-H-X(14)-[YF]-X(4)-S-X(23)-[RK]
绝对模式(SEQ ID NO:268)
G-X-W-X-X-X-E-D-X-X-[IL]-[RK]-X-X-X-X-X-X-G-X(23)-R-W-[IL]-N-Y-L-R-P-X-[IL]-[RK]-H-G-X-[FY]-X-X-X-E-[DE]-X(13)-W-X-X-X-A-X-X-X-X-X-R-T
可以通过序列识别号266的一致序列对MYB-亚群14序列进行定义。在所述的MYB-亚群14序列中,通过发现排他性的氨基酸残基或者支配性的氨基酸残基,从而对所述的氨基酸残基所在的位置进行识别。在下面的描述中,所有的位置的数字是参照AtMYB68蛋白(SEQ ID NO:2)而言的,所述的蛋白与上述的一般的一致序列,序列识别号266相关联。
在所述的R2结构域中的位置26上,在MYB-亚群14中存在一个保守的带阳性电荷的(K或者R)残基。尽管这个氨基酸在这一位置上对于这个亚群而言不是排他性的,但所述的其余AtMYB蛋白的趋势是一种疏水性的残基,在所有的MYB蛋白中有超过50%的蛋白具有一个疏水性的异亮氨酸残基或者缬氨酸残基(参见Stracke等人于2001年发表的文章)。
亚群14中的全部成员在位置36上含有一个插入,形成了一个额外的氨基酸残基。这对于MYB-亚群14而言似乎是排他性的。在所有的情况下,甘氨酸(G),一个小的疏水性残基,是所述的额外的残基,除了在MYB 87(SEQ ID NO:32)中以及在一种水稻同系物(SEQ IDNO:194)中,在所述的MYB 87中,这一位置上含有一个天冬酰胺残基,而在所述的水稻同系物中,这一位置上含有一个银色金属(argentine)。
所述的MYB-亚群14成员在位置39上支配性的含有一个疏水性的异亮氨酸残基。其中一个例外是一种被鉴定为序列识别号191的水稻同系物,所述的水稻同系物在这一位置上具有一个谷氨酰胺。尽管在MYB蛋白的这一位置上通常会发现其他的疏水性残基,但异亮氨酸对于MYB-亚群14而言似乎是排他性的。除此之外,在这一位置上,带阳性电荷的残基(39%R)以及极性残基(23%N)是最普遍的。
在所述的R3结构域中的位置68上,所有的MYB-亚群14成员含有所述的带阳性电荷的组氨酸残基。一种带阳性电荷的残基出现在所有的MYB蛋白中的这一位置上;然而与所述的MYB-亚群14相反的是,在其他的MYB蛋白中73%含有一个精氨酸(R)残基并且17%含有一个赖氨酸残基。
在位置83上,MYB-亚群14的大部分成员含有一个芳香族的疏水性残基(酪氨酸Y,或者苯丙氨酸F)。芳香族疏水性残基在这一位置上的出现对于MYB-亚群14而言似乎是排他性的。大部分的MYB蛋白具有一个组氨酸残基(87%)。已经提出这个组氨酸是存在于所述的螺旋的疏水性核中的一个至关重要的残基(参见Ogata等人于1992年发表的文章)。经过核磁共振(NMR)分析,所述的组氨酸似乎与两个临界的(critical)色氨酸残基进行了接触。在位置83上不存在组氨酸并不必须将其排除在MYB-亚群14的成员之外,因为已经识别出有些成员不具有组氨酸残基,例如序列识别号为169以及序列识别号为225的成员。
在位置88上,MYB-亚群14的成员含有所述的极性丝氨酸残基,而几乎所有的其他MYB蛋白(91%)含有所述的极性天冬酰胺残基。丝氨酸残基对于这个亚群而言似乎是排他性的。在位置88上不存在丝氨酸并不必须将其排除在MYB-亚群14的成员之外,因为已经识别出至少一个MYB-亚群14的成员在位置88上具有一个苯丙氨酸残基,例如序列识别号为256的成员。
在位置112上,MYB-亚群14的成员含有一个带阳性电荷的精氨酸残基或者赖氨酸残基。大部分的MYB蛋白在这一位置上同样含有带阳性电荷的残基,然而,组氨酸(48%)是已经发现的最普遍的残基。在位置112上不存在精氨酸并不必须将其排除在MYB-亚群14的成员之外,因为已经识别出至少一个MYB-亚群14的成员在位置112上具有一个谷氨酸残基,例如含有一个谷氨酸残基的序列识别号为68的成员以及含有一个苏氨酸的序列识别号为191的成员。
在R2R3结构域中存在变异是可以允许的,正如在所述的被识别出的一致序列中所表示出的。相对于所呈现的一致序列而言,一个MYB-亚群14序列的R2R3结构域可以是90%同源的,优选是95%同源的或者更加优选是99%同源的。
S1基序以及S2基序的加入
在MYB-亚群14中,所述的MYB68序列以及MYB84序列中含有两个更加保守的基序,所述的基序被鉴定为S1(SFSQLLLDPN)(SEQ ID NO:269)以及S2(TSTSADQSTISWEDI)(SEQ IDNO:270)。在AtMYB68序列、AtMYB36序列和AtMYB84序列以及芸薹属MYB68序列、芸薹属MYB36序列和芸薹属MYB84序列中都发现了这些基序,并且这些氨基酸序列表现出了至少70%的同源性。除此之外,已经发现,在油菜(Brassica napus)(Canola)、芜菁(Brassica rapa)(卷心菜)、甘蓝(Brassica oleracea)、野芥菜(Raphanus raphanistrum)(小萝卜)中的直系同源基因中存在与所述的S1基序或者S2基序的同源性,并且在枳壳(Poncirustrifoliate)(橘)的S2区域中存在同源性,而在截形苜蓿(Medicago truncatula)的同系物以及葡萄(Vitis Vinifera)(葡萄)的同系物的S2区域中存在微弱的同源性。当被作为S 1基序或者S2基序而被包括在内时,目标序列应当表现出具有至少70%的同源性,更加优选为80%并且最优选为90%。
来自于不属于拟南芥以及芸薹属的物种中的MYB68序列以及MYB84序列可能不含有S1基序以及S2基序,但仍然可能被划分为一种MYB亚群-14序列,这是根据序列分析以及考虑到其他标准来进行划分的。
MYB-亚群14成员的识别,包括MYB68,同系物
可以使用本领域技术人员已知的各种公共软件或者商业软件来寻找AtMYB-亚群14序列的同系物(表格1)或者一种目标MYB68(SEQ ID NO:1,SEQ ID NO:2)。可以利用基础性局部相似性比对搜索工具(Blast)进行比对并且对推定的序列进行识别。可以按照这里所概括的方法进行搜索。使用例如tblastn、tblastp这样的程序确定具有最高同源性的同系物,针对从美国国立生物信息中心(NCBI)处获得的数据库以及其他的基因组数据库进行搜索,其中所述的从美国国立生物信息中心(NCBI)处获得的数据库是例如表达序列标签数据库(EST),基因组综述序列数据库(GSS),高通量基因组数据库(HTG)以及染色体数据库,所述的其他基因组数据库是例如所述的美国基因组研究所(TIGR)人类基因序列集成(unigene)数据库,葫芦(Cucurbit)基因组学数据库(http://www.icugi.org/),向日葵以及莴苣(http://compgenomics.ucdavis.edu/compositae index.php),截形苜蓿(国际苜蓿基因组注释机构),SGN(http://www.sgn.cornell.edu/),以及橘(http:// harvest.ucr.edu/)。当物种具有更高程度的歧化时,在此情形下可以对所述的比对参数进行适当的变化,其中所述的比对参数是例如需要的缺口数量(Gap costs),矩阵值(matrixvalues)。
为了证实在每个物种中最接近AtMYB68的hit基因事实上是一种MYB68同系物,可以进行双向基础性局部相似性比对搜索,在所述的搜索中针对所有的拟南芥蛋白进行上述同系物的基础性局部相似性比对搜索。在许多情况中,所述的同系物中的最接近拟南芥的hit基因是一种MYB68基因家族成员(MYB亚群-14),而不是MYB68本身。如果所述的同系物与一种拟南芥蛋白最接近,而所述的拟南芥蛋白不在所述的MYB68基因家族的范围之内,在这种情况下,所述的同系物被评定为不属于MYB-亚群14中的成员。
可以使用例如“getorf”这样的程序对开放阅读框进行确定,其中所述的“getorf”程序来自于所述的欧洲分子生物学开放软件包(EMBOSS)程序,或者表达序列标签数据库扫描(ESTScan)。
识别MYB序列的方法、将MYB序列划分成指定的亚群的方法以及识别MYB-亚群14序列的方法在Stracke等人于2001年发表的文章中以及在Kranz等人于1998年发表的文章中进行了进一步的描述。
利用传统的基础性局部相似性比对搜索工具(blast)的方法通常不能找到在高度歧化的物种之间存在的具有微弱的保守性的同系物。在这样的情况下,在同系物之间将存在保守的基序、结构域以及指纹(fingerprint),并且这些物质是很好的功能同源性的预报者。存在许多精通于在物种间寻找保守性结构域的程序,所述的程序使用到隐式马尔可夫模型,位置特异性得分矩阵,以及模式。
PSI-Blast,PRATT,PHI-Blast,以及HMMBuild/HMMSearch
PRATT是一种由所述的蛋白质功能位点(PROSITE)数据库提供的工具。它建立了来自于一组保守性蛋白质的保守性模式http://www.expasy.ch/prosite/。利用PRATT来确定MYB68与同其最接近的同系物之间的保守性模式。之后利用ScanProsite以及PHI-Blast分别在所述的Swiss-Prot以及美国国立生物信息中心(NCBI)蛋白数据库中寻找所述的保守性模式。所述的搜索结果限于发生在同样含有所述的模式之间的比对。
利用HMMBuild来建立一种隐式马尔可夫模型,其中使用到所述的MYB68及其同系物。利用所述的隐式马尔可夫模型,通过HMMSearch在所述的美国国立生物信息中心的蛋白数据库中进行扫描。能够找到与所述的基础blastp搜索相类似的蛋白。
利用上述的方法,在超过50种不同的植物物种中发现了MYB68的同系物。在大多数的情形中,同源性被限制于所述的N-末端MYB DNA结合结构域中。在油菜(Brassica napus)(Canola)、芜菁(Brassica rapa)(卷心菜)、甘蓝(Brassica oleracea)、野芥菜(Raphanusraphanistrum)(小萝卜)、枳壳(Poncirus trifoliate)(橘)中发现的基因中,在所述的保守性较弱的C-末端区域中存在同源性,而在截形苜蓿(Medicago truncatula)的同系物以及葡萄(Vitis Vinifera)(葡萄)中发现的基因中,在所述的S2区域内存在微弱的同源性。
通过下载强有力的表达序列标签(EST)hits基因,并且使用CAP3对它们进行装配,从而对所述的枳壳(Poncirus trifoliate)以及芜菁(Brassica rapa)基因进行识别。上述得到的重叠群不能编码一个完整的蛋白。来自于橘的所述重叠群序列编码了一个仅仅跨越所述的S2区域的部分蛋白,而在芜菁(Brassica rapa)中,发现了一个具有202个氨基酸的部分蛋白,所述的部分蛋白跨越了AtMYB68中的1-202位置。
各种不同的程序已经利用数据图、模式、以及隐式马尔可夫模型对所述的MYB结构域进行了定义。为了证实所述的MYB结构域在一个未知的序列中的存在,可以针对这些数据图对这个序列进行搜索。InterProScan是格外有效的,因为它提供了一种能够同时在13个程序中进行查询的界面。被整合在InterPro中的所述数据库包括
a)ProDom:一个蛋白质结构域家族的数据库。是通过对来自于Swiss-Prot/Trembl(欧洲分子生物学实验室)数据库中的同源性片段进行聚集而建立的,并且随后进行递归性的PSI-BLAST(位置特异性叠代基础性局部相似性比对搜索工具)搜索。
b)HMMTIGR:由隐式马尔可夫模型所表示的蛋白质家族。
c)TMHMM:对蛋白质中的跨膜螺旋的预测。
d)FPrintScan:对指纹(fingerprints)PRINTS数据库进行的检索。指纹是由多个基序所表示的蛋白质家族。
e)ProfileScan:来自于家族相关性序列的数据图。
f)HMMPanther:一个隐式马尔可夫模型的数据库。
g)HMMPIR:基于完整蛋白的进化关系的隐式马尔可夫模型。
h)ScanRegExp:对所述的模式以及数据图的蛋白位点数据库进行扫描。
i)Gene3D:一个含有功能信息的蛋白质数据库。
j)HMMPfam:由隐式马尔可夫模型所表示的蛋白质结构域家族。
k)Superfamily:一个对所有的经过完整测序的有机体进行结构蛋白以及功能蛋白的标注的数据库。
l)HMMSmart:允许以隐式马尔可夫模型为基础对移动的结构域进行识别。
m)SignalIP:预测在氨基酸序列中是否存在信号肽的断裂位点并且定位所述的信号肽断裂位点在氨基酸序列中的位置。
对应于所述的蛋白中具有最高保守性的区域,将块(blocks)乘以进行比对的不带有缺口的片段。利用三个块对所述的MYB结构域进行表示。正如所预期的,AtMYB68含有这三个块中的每一个,以及一个5Wos2块。
本发明在一定程度上是以发现了具有热胁迫耐受性的植物作为基础的。形成所述的热耐受性表现型的基因已经被确定并且被证明是一个MYB68基因。这里公开了制备一种热耐受性转基因植物的方法。具体的,本发明识别了一个转录因子基因家族,具体的说是所述的MYB基因家族,并且特别的说是一个MYB-亚群14,当所述的MYB-亚群14在植物中进行表达时,使植物具有热胁迫耐受性并且所述的植物在经过热胁迫之后具有增加的产量或者显示出对干旱胁迫或者盐胁迫所具有的耐受性。
实施例10:MYB68同系物的识别
利用数据库序列搜索工具对来自于相同的植物物种、不同的植物物种或者其他有机体的同系物进行识别,其中所述的数据库序列搜索工具是例如所述的基础性局部相似性比对搜索工具(BLAST)(参见Altschul等人于1990年在J.Mol.Biol.《分子生物学杂志》215:403-410中发表的文章;以及Altschul等人于1997年在Nucl.Acid Res.《核酸研究》25:3389-3402中发表的文章)。利用所述的BLOSUM-62得分矩阵,对所述的tblastn或者blastn序列分析程序进行使用(参见Henikoff S.以及Henikoff J.G.于1992年在Proc.Natl.Acad.Sci.USA《美国科学院院刊》89:10915-10919中发表的文章)。所述的基础性局部相似性比对搜索工具(BLAST)报告的给出提供了一个得分,所述的得分考虑了相似残基或者相同残基的比对以及为了对所述的序列进行比对而需要的任何缺口。所述的得分矩阵为任何可能的序列对间进行的比对指定了一个得分。所述的P值反映了期望看到一个偶然发生的得分的次数。较高的得分是优选的并且一个低的阈值P值的阈值是优选的。这些是所述的序列识别标准。所述的tblastn序列分析程序被用来在一个核苷酸数据库中的六种翻译方式的序列中对一种多肽序列进行查询。当遇到P值小于-25,优选小于-70,并且更加优选小于-100时,将其鉴定为一种同源序列(范例性的选择性的序列标准)。所述的blastn序列分析程序被用来在一个核苷酸序列数据库中对一种核苷酸序列进行查询。同样,在这种情形中,较高的得分是优选的并且一个优选的阈值P值小于-13,优选小于-50,并且更加优选小于-100。
可以选择的,通过随机引物法对来自于具有下述序列识别号的序列的片段进行32磷的放射性标记(参见Sambrook等人于1989年编写的Molecular Cloning.A LaboratoryManual《分子克隆实验室手册》,第二版,Cold Spring Harbor Laboratory Press冷泉港实验室出版社,New York纽约),并且利用其进行一种植物基因组文库的筛选(范例性的受试多核苷酸),其中所述的序列是:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。作为一个例子,依照Stockinger等人所描述的方法(参见Stockinger E.J.等人于1996年在J.Heredity《遗传学杂志》87:214-218中发表的文章),从拟南芥(Arabidopsis thaliana)、烟草(Nicotiana tabacum)、醋栗番茄(Lycopersiconpimpinellifolium)、甜樱桃(Prunu savium)、酸樱桃(Prunus sativus)、或者水稻(Oryzasativa)中分离出植物总DNA。使大约2至10微克的每种DNA的样本进行限制性消化,将其转移至尼龙膜上(Micron Separations,Westboro,Mass.)并且进行杂交。杂交条件为:42℃,在由下述物质组成的溶液中:50%的甲酰胺,5倍的柠檬酸钠盐缓冲液(SSC),20毫摩磷酸盐缓冲液1倍的Denhardt’s,10%的硫酸葡聚糖,以及100微克/毫升的鲱鱼精DNA。在室温(RT)下利用下述物质进行四次低度严格的洗涤:2倍的柠檬酸钠盐缓冲液(SSC),0.05%的十二烷基肌氨酸钠以及0.02%的焦磷酸钠,在此之后在55℃下利用下述物质进行高度严格的洗涤::0.2倍的柠檬酸钠盐缓冲液(SSC),0.05%的十二烷基肌氨酸钠以及0.02%的焦磷酸钠。进行高度严格的洗涤直至依照Walling等人所描述的方法(参见Walling L.L等人于1988年在Nucl.Acids Res.《核酸研究》16:10477-10492中发表的文章)在所述的洗脱液中探测不到可以计算的物质。
实施例11:MYB68的技术特征的鉴定
可以利用识别基因对MYB68基因进行识别,其中所述的识别基因与AtMYB68(SEQID NO:1)具有高度的同源性。除了与所述的核苷酸序列或者氨基酸序列具有同源性之外,我们可以对备选基因进行识别,其中所述的备选基因共享构象蛋白结构。这样的结构基序能够帮助进行相关蛋白的识别,并且对所述的相关蛋白的结构与功能间的关系进行鉴定。
实施例12:同系物的功能确认
将备选的同系物导入到拟南芥中并且对热耐受性进行评价。使由具有下述序列识别号的序列所公开的基因在拟南芥植物中进行表达并且按照这里所描述的方法对热耐受性进行评价,其中所述的序列为:序列1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33,35,37,38,40,42,44,46,48,50,52,54,56,58,59,61,63,65,67,69,71,73,75,77,79,81,83,85,87,88,90,91,92,94,96,98,100,102,104,106,108,109,110,112,114,116,118,120,122,124,125,127,129,130,132,134,135,137,139,141,143,145,147,149,151,153,155,156,158,160,162,164,166,168,170,172,174,176,178,180,182,184,186,188,190,192,194,196,198,200,202,204,206,208,210,211,213,214,216,217,218,220,222,224,226,228,230,232,234,236,238,240,242,244,246,247,249,251,253,255,257,259,261,263以及265。任选的,如果一个备选的MYB-亚群14序列与所述的一般模式相互匹配,并且其进一步的包括一种与所述的排他模式之间的匹配,那么所述的序列是一种被包括在内的有力的备选者,成为一个MYB-亚群14序列或者MYB68基因,可以在任何可转化的物种中对上述序列的表达进行评价,其中所述的物种是例如芸薹属,玉米,棉花,大豆或者水稻。在本发明公开中已经提供了这样的功能性测试的例子。
值得注意的是,在这里所公开的某些序列不是完整长度的。可以通过本领域技术人员已知的标准分子方法获得完整长度的序列。之后可以按照这里所描述的方法对所述的完整长度的序列进行表达。
<110>波夫曼斯种植公司
<120>对于热胁迫具有增强的耐受力的植物
<130>22542-014001WO
<150>60/925312
<151>2007-04-18
<150>60/965582
<151>2007-08-20
<160>325
<170>PatentIn version 3.5
<210>1
<211>1125
<212>DNA
<213>拟南芥
<400>1
atgggaagag caccgtgttg tgataaggcc aacgtgaaga aagggccttg gtctcctgag 60
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ttgcctcaga aaattggttt aaggagatgt gggaagagtt gcaggctaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctccgagg aagaagacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaatt gccgggaaga 300
accgacaacg atatcaaaaa ctattggaac acgaggctga agaagaagct tctgaacaaa 360
caaaggaaag agttccaaga agcgcgaatg aagcaagaga tggtgatgat gaaaaggcaa 420
caacaaggac aaggacaagg tcaaagtaat ggtagtacgg atctttatct taacaacatg 480
tttggatcat caccatggcc attactacca caacttcctc ctccacatca tcaaatacct 540
cttggaatga tggaaccaac aagctgtaac tactaccaaa cgacaccgtc ttgtaaccta 600
gaacaaaagc cattgatcac actcaagaac atggtcaaga ttgaagaaga acaggaaagg 660
acaaaccctg atcatcatca tcaagattct gtcacaaacc cttttgattt ctctttctct 720
cagcttttgt tagatcccaa ttactatctg ggatcaggag ggggaggaga aggagatttt 780
gctatcatga gcagcagcac aaactcacca ttaccaaaca caagtagtga tcaacatcca 840
agtcaacagc aagagattct tcaatggttt gggagcagta actttcagac agaagcaatc 900
aacgatatgt tcataaacaa caacaacaac atagtgaatc ttgagaccat cgagaacaca 960
aaagtctatg gagacgcctc agtagccgga gccgctgtcc gagcagcttt gggcggaggg 1020
acaacgagta catcggcgga tcaaagtaca ataagttggg aggatataac ttctctagtt 1080
aattccgaag atgcaagtta cttcaatgcg ccaaatcatg tgtaa 1125
<210>2
<211>374
<212>PRT
<213>拟南芥
<400>2
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Gly Gln
130 135 140
Gly Gln Gly Gln Ser Asn Gly Ser Thr Asp Leu Tyr Leu Asn Asn Met
145 150 155 160
Phe Gly Ser Ser Pro Trp Pro Leu Leu Pro Gln Leu Pro Pro Pro His
165 170 175
His Gln Ile Pro Leu Gly Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr
180 185 190
Gln Thr Thr Pro Ser Cys Asn Leu Glu Gln Lys Pro Leu Ile Thr Leu
195 200 205
Lys Asn Met Val Lys Ile Glu Glu Gln Gln Glu Arg Thr Asn Pro Asp
210 215 220
His His His Gln Asp Ser Val Thr Asn Pro Phe Asp Phe Ser Phe Ser
225 230 235 240
Gln Leu Leu Leu Asp Pro Asn Tyr Tyr Leu Gly Ser Gly Gly Gly Gly
245 250 255
Glu Gly Asp Phe Ala Ile Met Ser Ser Ser Thr Asn Ser Pro Leu Pro
260 265 270
Asn Thr Ser Ser Asp Gln His Pro Ser Gln Gln Gln Glu Ile Leu Gln
275 280 285
Trp Phe Gly Ser Ser Asn Phe Gln Thr Glu Ala Ile Asn Asp Met Phe
290 295 300
Ile Asn Asn Asn Asn Asn Ile Val Asn Leu Glu Thr Ile Glu Asn Thr
305 310 315 320
Lys Val Tyr Gly Asp Ala Ser Val Ala Gly Ala Ala Val Arg Ala Ala
325 330 335
Leu Gly Gly Gly Thr Thr Ser Thr Ser Ala Asp Gln Ser Thr Ile Ser
340 345 350
Trp Glu Asp Ile Thr Ser Leu Val Asn Ser Glu Asp Ala Ser Tyr Phe
355 360 365
Asn Ala Pro Asn His Val
370
<210>3
<211>933
<212>DNA
<213>拟南芥
<400>3
atgggaagag caccgtgttg tgacaaagca aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcaa aactcaaatc ttacattgaa aatagtggca ccggaggcaa ttggatcgct 120
ttgcctcaaa agattggttt aaagagatgt ggaaagagtt gcaggctgag gtggcttaac 180
tatcttagac caaacatcaa acatggtggc ttctctgagg aagaagaaaa catcatttgt 240
agcctttacc ttacaattgg tagcaggtgg tctataatcg ctgctcaatt gccgggacga 300
acagacaacg atataaaaaa ctattggaac acgaggctca agaagaaact cattaacaaa 360
caacgcaagg agcttcaaga agcttgtatg gagcagcaag agatgatggt gatgatgaag 420
agacaacacc aacaacaaca aatccaaact tcttttatga tgagacaaga ccaaacaatg 480
ttcacatggc cactacatca tcataatgtt caagttccag ctcttttcat gaatcaaacc 540
aactcgtttt gcgaccaaga agatgttaag ccagtgctca tcaagaacat ggtcaagatc 600
gaagatcaag aactggagaa aacaaaccct catcatcatc aagattcaat gacaaacgct 660
tttgatcatc tctctttctc tcaactcttg ttagatccta atcataacca cttaggatca 720
ggcgagggtt tctccatgaa ctctatcttg agcgccaaca caaactctcc attgcttaat 780
acaagtaatg ataatcagtg gttcgggaat ttccaggccg aaaccgtaaa cttgttctca 840
ggagcctcca caagtacttc ggcagatcaa agcactataa gttgggaaga cataagctct 900
cttgtttatt ctgattcaaa gcaatttttt taa 933
<210>4
<211>310
<212>PRT
<213>拟南芥
<400>4
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Glu Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Leu Thr Ile Gly Ser Arg Trp Ser Ile IleAla Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Ile Asn Lys Gln Arg Lys Glu Leu Gln Glu Ala
115 120 125
Cys Met Glu Gln Gln Glu Met Met Val Met Met Lys Arg Gln His Gln
130 135 140
Gln Gln Gln Ile Gln Thr Ser Phe Met Met Arg Gln Asp Gln Thr Met
145 150 155 160
Phe Thr Trp Pro Leu His His His Asn Val Gln Val Pro Ala Leu Phe
165 170 175
Met Asn Gln Thr Asn Ser Phe Cys Asp Gln Glu Asp Val Lys Pro Val
180 185 190
Leu Ile Lys Asn Met Val Lys Ile Glu Asp Gln Glu Leu Glu Lys Thr
195 200 205
Asn Pro His His His Gln Asp Ser Met Thr Asn Ala Phe Asp His Leu
210 215 220
Ser Phe Ser Gln Leu Leu Leu Asp Pro Asn His Asn His Leu Gly Ser
225 230 235 240
Gly Glu Gly Phe Ser Met Asn Ser Ile Leu Ser Ala Asn Thr Asn Ser
245 250 255
Pro Leu Leu Asn Thr Ser Asn Asp Asn Gln Trp Phe Gly Asn Phe Gln
260 265 270
Ala Glu Thr Val Asn Leu Phe Ser Gly Ala Ser Thr Ser Thr Ser Ala
275 280 285
Asp Gln Ser Thr Ile Ser Trp Glu Asp Ile Ser Ser Leu Val Tyr Ser
290 295 300
Asp Ser Lys Gln Phe Phe
305 310
<210>5
<211>1002
<212>DNA
<213>拟南芥
<400>5
atgggaagag ctccatgctg cgacaaggca aacgtgaaga aaggaccatg gtcaccggaa 60
gaagatgtga agctcaagga ttacatcgac aaatatggca ctggtggcaa ctggatcgca 120
ctgcctcaga aaattgggct gaagagatgt ggtaagagtt gcagactgag atggcttaat 180
tacttaagac caaacatcaa acatggtggt ttttctgagg aagaagatag aatcatcttg 240
agtctctaca ttagcattgg aagccggtgg tccataattg cagctcagct tcctggaagg 300
actgacaatg atatcaagaa ttattggaac acaaaactga agaagaaact tctaggaaga 360
cagaaacaaa tgaatcgtca agactccata accgattcta ctgagaacaa cctcagcaac 420
aataacaaca ataagagtcc tcagaatctg agcaattcgg cactggagag gctccagctt 480
cacatgcagc ttcagaatct acagagccct ttctctagtt tctacaacaa ccctatcttg 540
tggcccaagc ttcatccatt gctccagagc actacaacta atcaaaaccc taagcttgca 600
tctcaagaaa gcttccaccc tttaggagtt aacgttgatc atcagcacaa caataccaag 660
ctagctcaga taaacaatgg agcctcttct ctctattcgg agaacgtaga gcaatcccaa 720
aaccctgctc atgaatttca acctaatttc ggtttttcac aggaccttcg attagataat 780
cataacatgg actttatgaa cagaggggtt tctaaagaac tgtttcaagt gggcaacgag 840
tttgagctaa cgaacggttc gagttggtgg tcagaggaag tggaactaga gaggaaaacg 900
acgtcgtcga gttcttgggg gtcagcttct gtgcttgatc agacaactga gggaatggtt 960
atgcttcaag attacgctca gatgagctac cacagtgttt aa 1002
<210>6
<211>333
<212>PRT
<213>拟南芥
<400>6
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Val Lys Leu Lys Asp Tyr Ile Asp Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Gln Lys Gln Met Asn Arg Gln Asp
115 120 125
Ser Ile Thr Asp Ser Thr Glu Asn Asn Leu Ser Asn Asn Asn Asn Asn
130 135 140
Lys Ser Pro Gln Asn Leu Ser Asn Ser Ala Leu Glu Arg Leu Gln Leu
145 150 155 160
His Met Gln Leu Gln Asn Leu Gln Ser Pro Phe Ser Ser Phe Tyr Asn
165 170 175
Asn Pro Ile Leu Trp Pro Lys Leu His Pro Leu Leu Gln Ser Thr Thr
180 185 190
Thr Asn Gln Asn Pro Lys Leu Ala Ser Gln Glu Ser Phe His Pro Leu
195 200 205
Gly Val Asn Val Asp His Gln His Asn Asn Thr Lys Leu Ala Gln Ile
210 215 220
Asn Asn Gly Ala Ser Ser Leu Tyr Ser Glu Asn Val Glu Gln Ser Gln
225 230 235 240
Asn Pro Ala His Glu Phe Gln Pro Asn Phe Gly Phe Ser Gln Asp Leu
245 250 255
Arg Leu Asp Asn His Asn Met Asp Phe Met Asn Arg Gly Val Ser Lys
260 265 270
Glu Leu Phe Gln Val Gly Asn Glu Phe Glu Leu Thr Asn Gly Ser Ser
275 280 285
Trp Trp Ser Glu Glu Val Glu Leu Glu Arg Lys Thr Thr Ser Ser Ser
290 295 300
Ser Trp Gly Ser Ala Ser Val Leu Asp Gln Thr Thr Glu Gly Met Val
305 310 315 320
Met Leu Gln Asp Tyr Ala Gln Met Ser Tyr His Ser Val
325 330
<210>7
<211>1092
<212>DNA
<213>芜菁
<400>7
atgggaagag caccgtgttg tgacaaggct aacgtgaaga aagggccgtg gtctcctgaa 60
gaagacgcaa aactcaaaga ttacatcgag aataatggca caggcggcaa ctggattgcg 120
ttgcctcaga agattggtct aagaagatgt gggaagagtt gcagactaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aagaggacaa catcatttgt 240
aatctctatg ttaccattgg tagcaggtgg tctataattg ctgcacaatt gcctggaaga 300
acagacaatg atatcaagaa ctattggaac acgaggctga agaagaagct tcttaacaaa 360
caaagaaaag agtaccaaga agctcggatg aagcaagata tggtgatgat aaagcgacaa 420
gaacaaggga caggccaaag taatgctagt agggatcttt attcgaacaa catgtttgga 480
tcatcaccat ggccattact acaacagctt cctcctcatc atcaagtacc tcttgtgatg 540
atggaaccaa caagttgtaa ctactaccaa acgtcaccct cttgtaacct agaacaaaag 600
ccactgatca ctttcaagaa catggtcaag attgaagaag aaccggagag aacaaaccct 660
tataatcctc agcatcaaaa ttctatcaca aacccttttg atgtctcctt ctcccagctc 720
ttgttagatc ctaattacta cttaggatca ggaggaggag cagaagggga ttttgctatc 780
atgagtagca gcacaaattc tccattacca aacacaagtg gtgatcaaaa tgaacatcag 840
cagcaagaga ttcttcaatg gtttgggagt agtaatcttc agacagaagc aagcagtgat 900
atgttcttaa acaacatagg gaatcttgag accaacgagg acacaagatt ctactcatca 960
ttagccggtg ttggagcggc tttggccgga ggaacgacga gtacatcggc agatcaaagc 1020
acaataagtt gggaggacat aacatctctt gttaattccg aagatgcaag ttacttcaat 1080
gggccaaatt aa 1092
<210>8
<211>363
<212>PRT
<213>芜菁
<400>8
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Tyr Gln Glu Ala
115 120 125
Arg Met Lys Gln Asp Met Val Met Ile Lys Arg Gln Glu Gln Gly Thr
130 135 140
Gly Gln Ser Asn Ala Ser Arg Asp Leu Tyr Ser Asn Asn Met Phe Gly
145 150 155 160
Ser Ser Pro Trp Pro Leu Leu Gln Gln Leu Pro Pro His His Gln Val
165 170 175
Pro Leu Val Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr Gln Thr Ser
180 185 190
Pro Ser Cys Asn Leu Glu Gln Lys Pro Leu Ile Thr Phe Lys Asn Met
195 200 205
Val Lys Ile Glu Glu Glu Pro Glu Arg Thr Asn Pro Tyr Asn Pro Gln
210 215 220
His Gln Asn Ser Ile Thr Asn Pro Phe Asp Val Ser Phe Ser Gln Leu
225 230 235 240
Leu Leu Asp Pro Asn Tyr Tyr Leu Gly Ser Gly Gly Gly Ala Glu Gly
245 250 255
Asp Phe Ala Ile Met Ser Ser Ser Thr Asn Ser Pro Leu Pro Asn Thr
260 265 270
Ser Gly Asp Gln Asn Glu His Gln Gln Gln Glu Ile Leu Gln Trp Phe
275 280 285
Gly Ser Ser Asn Leu Gln Thr Glu Ala Ser Ser Asp Met Phe Leu Asn
290 295 300
Asn Ile Gly Asn Leu Glu Thr Asn Glu Asp Thr Arg Phe Tyr Ser Ser
305 310 315 320
Leu Ala Gly Val Gly Ala Ala Leu Ala Gly Gly Thr Thr Ser Thr Ser
325 330 335
Ala Asp Gln Ser Thr Ile Ser Trp Glu Asp Ile Thr Ser Leu Val Asn
340 345 350
Ser Glu Asp Ala Ser Tyr Phe Asn Gly Pro Asn
355 360
<210>9
<211>966
<212>DNA
<213>水稻
<400>9
atggggagag cgccgtgctg cgacaaggcg agcgtgaaga aggggccatg gtcaccggag 60
gaggacgcga agctcaaatc ctacatcgag cagaacggca ccggcggcaa ctggatcgcc 120
ctgccccaga agatcggttt gaaaaggtgc ggcaagagct gccgcctccg gtggctgaac 180
tacctccggc cgaacatcaa gcacggcggg ttctcggagg aggaagacag gatcatcctc 240
agcctctaca tcagcatagg aagcaggtgg tcgataatag cagcgcagct gccggggcgg 300
acggacaatg acatcaagaa ctattggaac acgaggctca agaagaagct ctttggcaag 360
cagtcgcgca aggatcagag gcagcagcag cacctggcgc gccaggcggc agcagctgcc 420
agcgacttgc agatcaaaca agaagcgagc aggggtgcaa acgaagccga tggcttggct 480
gccggtgcca attacacttg gcatcaccac cacgccatgg ccgtgcctgt gcacccgatg 540
tcggcaccaa tggtggtgga aggaggccgt gtgggagacg atgtcgatga gtcgatccgg 600
aagcttctgt tcaagctcgg agggaaccca ttcgcggcct cgccggcacc gccatgcata 660
cctccaccac caatgtacga ggaagcccca agcttcgtgc caccattggc gcacggcgtg 720
ccgctcaacg aaggcggcat gcagtgctcc agcgtgctgc cggcgctgga gctggacgag 780
aacttccact tcaaccatgt caagctggac gggctcgagt gcctcttcgg gatgggagat 840
caccaaaaca tgagatggaa tgaggtgagc ccgttggttt gccctaataa cgctgtggcg 900
tccagctccc aagggatgca gcagtactgc ctagttgaag aaccagctga cctcgggatg 960
cagtag 966
<210>10
<211>321
<212>PRT
<213>水稻
<400>10
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys Gln Ser Arg Lys Asp Gln Arg Gln
115 120 125
Gln Gln His Leu Ala Arg Gln Ala Ala Ala Ala Ala Ser Asp Leu Gln
130 135 140
Ile Lys Gln Glu Ala Ser Arg Gly Ala Asn Glu Ala Asp Gly Leu Ala
145 150 155 160
Ala Gly Ala Asn Tyr Thr Trp His His His His Ala Met Ala Val Pro
165 170 175
Val His Pro Met Ser Ala Pro Met Val Val Glu Gly Gly Arg Val Gly
180 185 190
Asp Asp Val Asp Glu Ser Ile Arg Lys Leu Leu Phe Lys Leu Gly Gly
195 200 205
Asn Pro Phe Ala Ala Ser Pro Ala Pro Pro Cys Ile Pro Pro Pro Pro
210 215 220
Met Tyr Glu Glu Ala Pro Ser Phe Val Pro Pro Leu Ala His Gly Val
225 230 235 240
Pro Leu Asn Glu Gly Gly Met Gln Cys Ser Ser Val Leu Pro Ala Leu
245 250 255
Glu Leu Asp Glu Asn Phe His Phe Asn His Val Lys Leu Asp Gly Leu
260 265 270
Glu Cys Leu Phe Gly Met Gly Asp His Gln Asn Met Arg Trp Asn Glu
275 280 285
Val Ser Pro Leu Val Cys Pro Asn Asn Ala Val Ala Ser Ser Ser Gln
290 295 300
Gly Met Gln Gln Tyr Cys Leu Val Glu Glu Pro Ala Asp Leu Gly Met
305 310 315 320
Gln
<210>11
<211>1071
<212>DNA
<213>棉花属
<400>11
atggggagag ctccttgttg tgacaaagct aacgtcaaga aaggcccatg gtcacctgaa 60
gaagacacca agctcaaggc atacatcgag cagcatggca ctggcggaaa ctggatcgct 120
ttgcctcaca aaattggcct taagagatgt gggaagagct gccgcctcag atggttaaac 180
tatctccgcc caaatattaa gcatggagga ttctccgaag aagaagataa aattatttgc 240
agcctctata tcagtattgg gagcaggtgg tctattattg ctgcacaatt accggggagg 300
actgataacg atataaagaa ctattggaac acaaggctta agaagaagct tctgggcaag 360
caacgcaaag agcatcagtc tcgaagaggc aacagcctaa agcaagatat gaagagatca 420
tcagctagtg tgggggattc catggttcct gcagataaca tcaatcaaat cccttactgg 480
ccagagctgc ctgtgctggc tgcggcggcg gctcccatac cgcactcaag tcaagaacat 540
cgcattgaca gccaagcctc gatgagaaga ttactaatca agcttggggg aagattttct 600
gaggatgatc atgtggttaa tgatgggaca actcttcatc agtttcctaa tgatttatcc 660
actactgatc aggatcttta cgagcagact gtctatgtgc cctcttcttc ttcttcttct 720
cccatggatg ccttgagctt aagcaataac atcggttctc agtttgtgaa ctctcagttc 780
gccatagatg gagggaatct gcccattctg caaggacaaa gcactacttt ttcatcagag 840
ctccaggaaa tgggatatag cagcaaccca cagagattag atggaatgga gttcttatat 900
ggggaaggca tggtcgataa tagaggcgtg aatccttgtg aaagcattgg ctggggtgac 960
actagctctc tggttggccc tccttgtgct tcggaatatg gagtcatgca acaaggaatg 1020
cttcaagaat atggttttag tgagatgagg tacccaggag gagcgcaata g 1071
<210>12
<211>356
<212>PRT
<213>棉花属
<400>12
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Lys Leu Lys Ala Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu His Gln Ser Arg
115 120 125
Arg Gly Asn Ser Leu Lys Gln Asp Met Lys Arg Ser Ser Ala Ser Val
130 135 140
Gly Asp Ser Met Val Pro Ala Asp Asn Ile Asn Gln Ile Pro Tyr Trp
145 150 155 160
Pro Glu Leu Pro Val Leu Ala Ala Ala Ala Ala Pro Ile Pro His Ser
165 170 175
Ser Gln Glu His Arg Ile Asp Ser Gln Ala Ser Met Arg Arg Leu Leu
180 185 190
Ile Lys Leu Gly Gly Arg Phe Ser Glu Asp Asp His Val Val Asn Asp
195 200 205
Gly Thr Thr Leu His Gln Phe Pro Asn Asp Leu Ser Thr Thr Asp Gln
210 215 220
Asp Leu Tyr Glu Gln Thr Val Tyr Val Pro Ser Ser Ser Ser Ser Ser
225 230 235 240
Pro Met Asp Ala Leu Ser Leu Ser Asn Asn Ile Gly Ser Gln Phe Val
245 250 255
Asn Ser Gln Phe Ala Ile Asp Gly Gly Asn Leu Pro Ile Leu Gln Gly
260 265 270
Gln Ser Thr Thr Phe Ser Ser Glu Leu Gln Glu Met Gly Tyr Ser Ser
275 280 285
Asn Pro Gln Arg Leu Asp Gly Met Glu Phe Leu Tyr Gly Glu Gly Met
290 295 300
Val Asp Asn Arg Gly Val Asn Pro Cys Glu Ser Ile Gly Trp Gly Asp
305 310 315 320
Thr Ser Ser Leu Val Gly Pro Pro Cys Ala Ser Glu Tyr Gly Val Met
325 330 335
Gln Gln Gly Met Leu Gln Glu Tyr Gly Phe Ser Glu Met Arg Tyr Pro
340 345 350
Gly Gly Ala Gln
355
<210>13
<211>596
<212>DNA
<213>黑大豆
<400>13
atggggaggg caccttgctg tgacaaagca aacgtgaaga aaggtccttg gtcgccagaa 60
gaagatacca aactcaaatc ctacatcgaa cagcacggaa ccggaggaaa ctggatcgct 120
ttgccacaaa agattggcct taaacgatgt ggcaagagtt gccgtctcag gtggctaaac 180
tacctccgcc ctaacatcag acacggtggt ttctccgaag aagaagacaa catcatttgc 240
agcctctacg ttagcattgg aagcaggtgg tcggtcattg cagcacaatt gccgggaaga 300
actgataatg acataaagaa ctattggaac acgaggctga agaagaagct tctagggaaa 360
caccgtaagg aactacaggc acgcaacaaa ggaaacggtg gtatccttaa acaggagaac 420
agttcctcgc tcttgcttca gcaaaacagt gctcaacaat tacatccatg ttggccacag 480
attccagtgc tgccactttc atcaccgtac acaaaccaaa gtccaagttt caacgaccaa 540
gattccatta gaaagcttct aatcaagctt ggagggagat tctccgatga ttatca 596
<210>14
<211>198
<212>PRT
<213>黑大豆
<400>14
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys His Arg Lys Glu Leu Gln Ala Arg
115 120 125
Asn Lys Gly Asn Gly Gly Ile Leu Lys Gln Glu Asn Ser Ser Ser Leu
130 135 140
Leu Leu Gln Gln Asn Ser Ala Gln Gln Leu His Pro Cys Trp Pro Gln
145 150 155 160
Ile Pro Val Leu Pro Leu Ser Ser Pro Tyr Thr Asn Gln Ser Pro Ser
165 170 175
Phe Asn Asp Gln Asp Ser Ile Arg Lys Leu Leu Ile Lys Leu Gly Gly
180 185 190
Arg Phe Ser Asp Asp Tyr
195
<210>15
<211>1463
<212>DNA
<213>黑大豆
<400>15
aaataacact ctctctctct caattatttc tgccgtaaat agacaagggt tatctcaaat 60
aatcaatcta agctagctga aagcaacaac aacaacatgg ggagggcacc ttgctgtgac 120
aaatcaaacg tgaagaaagg cccttggtcg ccagatgaag atgccaaact caaatcctac 180
atcgaacagc acggaaccgg agggaactgg attgctttgc cacaaaagat tggcctcaaa 240
cgatgtggca agagttgccg tctcaggtgg ctaaactacc tccgccctaa catcagacac 300
ggtggtttct ccgaagaaga agacaacatc atttgcagcc tctacgttag cattggaagc 360
aggtggtcgg tcattgcagc acaattgccg ggaagaactg ataatgacat aaagaactat 420
tggaacacga ggctgaagaa gaagcttcta gggaaacacc gtaaggaact acaggcacgc 480
aacaaaggaa acggtggtat ccttaaacag gagaacagtt cctcgctctt gcttcagcaa 540
aacagtgctc aacaattaca tccatgttgg ccacagattc cagtgctgcc actttcatca 600
ccgtacacaa accaaagtcc aagtttcaac gaccaagatt ccattagaaa gcttctaatc 660
aagcttggag ggagattctc cgatgattat caacccatcc tagatgggtt gaatcttcag 720
ttcccacatg gttcaaattc tctctcatca acacaacaaa ttcaagagga gcaagtccat 780
gttggttctt ctgcactagg gtgcatgaac tctattggcc acaatcaagt acaatttggt 840
cagagtaatg agtactgtgc tgagttggtg caaggacaag ggagtttcat taccacagca 900
attggggaaa tggtttcttc taatgattat tctcgaaggt tattaggtgg gtcgttggag 960
ttcttctatg gggaggaaat gattactgat aataagataa tgggtgcttg tgcttcttca 1020
agttgtgggc aaagtactaa ttggggtgaa accagtagtt ctctgatgta tccttctctt 1080
gttgcttcga attacgaggg tgtgcggcaa gagatgccac gagaatgtgc ttttcaagag 1140
ttgagctacc cgggggcgca atagcatgtg tgtacattat ttgttatcaa ttggtggagg 1200
ttgggattat atgtatccaa attgaagcta gcaaaaggga attaactttg tatttgattc 1260
ccatatagat aataaaaatc agcttgattt atatataact agagacttgt ccgctatgtg 1320
agactttctc atgtgctgat cccttagtgg cagtgcagtt taattatctg atttgtttgt 1380
gtattatttg gttgtaatct tgagagtcat ttaaatatat ccttttatgt tccaagctaa 1440
aaaaaaaaaa aaagaaaacc ccc 1463
<210>16
<211>355
<212>PRT
<213>黑大豆
<400>16
Met Gly Arg Ala Pro Cys Cys Asp Lys Ser Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys His Arg Lys Glu Leu Gln Ala Arg
115 120 125
Asn Lys Gly Asn Gly Gly Ile Leu Lys Gln Glu Asn Ser Ser Ser Leu
130 135 140
Leu Leu Gln Gln Asn Ser Ala Gln Gln Leu His Pro Cys Trp Pro Gln
145 150 155 160
Ile Pro Val Leu Pro Leu Ser Ser Pro Tyr Thr Asn Gln Ser Pro Ser
165 170 175
Phe Asn Asp Gln Asp Ser Ile Arg Lys Leu Leu Ile Lys Leu Gly Gly
180 185 190
Arg Phe Ser Asp Asp Tyr Gln Pro Ile Leu Asp Gly Leu Asn Leu Gln
195 200 205
Phe Pro His Gly Ser Asn Ser Leu Ser Ser Thr Gln Gln Ile Gln Glu
210 215 220
Glu Gln Val His Val Gly Ser Ser Ala Leu Gly Cys Met Asn Ser Ile
225 230 235 240
Gly His Asn Gln Val Gln Phe Gly Gln Ser Asn Glu Tyr Cys Ala Glu
245 250 255
Leu Val Gln Gly Gln Gly Ser Phe Ile Thr Thr Ala Ile Gly Glu Met
260 265 270
Val Ser Ser Asn Asp Tyr Ser Arg Arg Leu Leu Gly Gly Ser Leu Glu
275 280 285
Phe Phe Tyr Gly Glu Glu Met Ile Thr Asp Asn Lys Ile Met Gly Ala
290 295 300
Cys Ala Ser Ser Ser Cys Gly Gln Ser Thr Asn Trp Gly Glu Thr Ser
305 310 315 320
Ser Ser Leu Met Tyr Pro Ser Leu Val Ala Ser Asn Tyr Glu Gly Val
325 330 335
Arg Gln Glu Met Pro Arg Glu Cys Ala Phe Gln Glu Leu Ser Tyr Pro
340 345 350
Gly Ala Gln
355
<210>17
<211>395
<212>DNA
<213>玉蜀黍
<400>17
atggggagag ctccgtgctg cgacaaggct actgtgaaga agggcccgtg gtcaccggag 60
gaggacgcca agctcaagtc ctacatcgag cagaacggca ccggcggtaa ctggatagcc 120
ctgccgcaga agataggttt gaagaggtgc ggcaagagct gccgcctccg gtggctcaac 180
tacctccggc caaacatcaa gcacggcggg ttctcggatg aggaggacat gataatcctt 240
agcctctaca tcagcatagg cagcaggtgg tcgataatag cggcgcagct gccgggaagg 300
acggacaacg acataaagaa ctactggaac acgaggctca agaagaagct cttcggcaag 360
ccgtttaaaa aaggtattcg gcagggttcc ttttt 395
<210>18
<211>131
<212>PRT
<213>玉蜀黍
<400>18
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Asp Glu Glu Asp Met Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys Pro Phe Lys Lys Gly Ile Arg Gln
115 120 125
Gly Ser Phe
130
<210>19
<211>471
<212>DNA
<213>高粱
<400>19
atggggagag ctccgtgctg cgacaaggct actgtgaaga agggtccgtg gtcgccggag 60
gaggacgcca agctcaagtc ctacatcgag cagaacggca ccggcggtaa ctggatagcc 120
ctgcctcaga agataggtct gaagaggtgt ggcaagagct gccgcctccg gtggctcaac 180
tacctccggc caaacatcaa gcacggtggg ttctccgagg aggaagacag aataatcctt 240
agcctctaca tcagcatagg cagcaggtgg tcgataatag cggcgcagct gccggggagg 300
acggacaatg acataaagaa ctactggaac acgaggctca agaagaagct cttcggcaag 360
caatcgcgca aggatcaacg gcagcatcag ttcatgcgcc agcaggcggc agcggcaaac 420
gatgggatga tgaagcaaga agcagcacac cccgggatgc agcccggagc a 471
<210>20
<211>157
<212>PRT
<213>高粱
<400>20
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys Gln Ser Arg Lys Asp Gln Arg Gln
115 120 125
His Gln Phe Met Arg Gln Gln Ala Ala Ala Ala Asn Asp Gly Met Met
130 135 140
Lys Gln Glu Ala Ala His Pro Gly Met Gln Pro Gly Ala
145 150 155
<210>21
<211>525
<212>DNA
<213>小麦
<400>21
atggggaggg cgccgtgctg cgacaaggcg acggtgaaga agggcccctg gtcgccggag 60
gaggacgcca agctcaaggc ctacatcgac gagaatggca ccggcggcaa ctggatcgcc 120
ctgccgcaga agatcgggct gaagaggtgc ggcaaaagct gtaggctcag atggctcaac 180
tatctgaggc caaacatcaa gcacggcgac ttcacagagg aagaggaaca catcatttgc 240
agcctctaca ttagcatcgg cagcaggtgg tcgatcatcg cggcgcagct gccgggcaga 300
acggacaacg acatcaagaa ctactggaac accaagctca agaagaagct cctcggcaag 360
cgcgcgccgt cccgccgcct gcagcgcgcc aaccaagacg cgccgatgcc ctactcctac 420
ctggcggcgg gcggcggcag cgccagcagc agcgggaacg ctagcggcac caccgcggca 480
ctcagctcgt cagcgctgga gcggatccag ctccacatgc gcctg 525
<210>22
<211>175
<212>PRT
<213>小麦
<400>22
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Asp Glu Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Thr Glu Glu Glu Glu His Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Arg Ala Pro Ser Arg Arg Leu Gln
115 120 125
Arg Ala Asn Gln Asp Ala Pro Met Pro Tyr Ser Tyr Leu Ala Ala Gly
130 135 140
Gly Gly Ser Ala Ser Ser Ser Gly Asn Ala Ser Gly Thr Thr Ala Ala
145 150 155 160
Leu Ser Ser Ser Ala Leu Glu Arg Ile Gln Leu His Met Arg Leu
165 170 175
<210>23
<211>1035
<212>DNA
<213>杨属
<400>23
atggggagag ctccttgctg tgacaaagcc aacgtgaaga aaggtccatg gtctcccgag 60
gaagatgcca tactcaaggc ctatattgag cagcatggaa ccggtggcaa tcggattgcc 120
ttgccacaga agataggcct gaaaaggtgt ggcaagagtt gtcgacttag atggttgaat 180
tatctccgtc caaacattaa acatggaggg ttttcagagg aagaagataa cattatttgc 240
agtctctata taagtattgg aagcaggtgg tctatcattg ctgctcagtt acctggaagg 300
accgataatg atataaaaaa ctactggaac acaaggctta agaagaagct cttaggaaaa 360
cagcgcaagg aacaagcagc ccggcgagct agtctcaagc aagaaatcat gacaaagaga 420
gaaataaacg agagcttcat ggttcctggg gctatccctc atcaacaaag cccttactgg 480
ccagaggtac cagctctagt catgaatcaa aaccaagatt ctcatttgat ggatcaagaa 540
tccattagga acttgctgat caaacttggt gggagatttt ctgacaataa tcaagagtca 600
gccctattca ctacagtcaa caattaccct ctcgacggtt caagcagaca agatcaagta 660
ccatacacaa actccataga tgtgctttct tcttcggcac ccatgcgatc aatggatagt 720
actaccagtt gttctcaatt tcctaatagc aactacaacg gtccaaatat gtgtcaagct 780
ggacttgaaa acttgttggt cgagctaggt ggattggtct gtagcaaccc acagcgtgta 840
gaaggtttgg atagtttcta cgggatggac atggctgcca gtggcagcac gggggctagt 900
tctgcagaaa gtaacagctg gggacatata agctctcttg gttatcctca gcttgtttct 960
gactatgaaa cttgcttgca aagcatgcca caagattcat cttttgaaga ttcaagcttc 1020
tttgggccac aatga 1035
<210>24
<211>344
<212>PRT
<213>杨属
<400>24
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Ile Leu Lys Ala Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Arg Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Ala Ala Arg
115 120 125
Arg Ala Ser Leu Lys Gln Glu Ile Met Thr Lys Arg Glu Ile Asn Glu
130 135 140
Ser Phe Met Val Pro Gly Ala Ile Pro His Gln Gln Ser Pro Tyr Trp
145 150 155 160
Pro Glu Val Pro Ala Leu Val Met Asn Gln Asn Gln Asp Ser His Leu
165 170 175
Met Asp Gln Glu Ser Ile Arg Asn Leu Leu Ile Lys Leu Gly Gly Arg
180 185 190
Phe Ser Asp Asn Asn Gln Glu Ser Ala Leu Phe Thr Thr Val Asn Asn
195 200 205
Tyr Pro Leu Asp Gly Ser Ser Arg Gln Asp Gln Val Pro Tyr Thr Asn
210 215 220
Ser Ile Asp Val Leu Ser Ser Ser Ala Pro Met Arg Ser Met Asp Ser
225 230 235 240
Thr Thr Ser Cys Ser Gln Phe Pro Asn Ser Asn Tyr Asn Gly Pro Asn
245 250 255
Met Cys Gln Ala Gly Leu Glu Asn Leu Leu Val Glu Leu Gly Gly Leu
260 265 270
Val Cys Ser Asn Pro Gln Arg Val Glu Gly Leu Asp Ser Phe Tyr Gly
275 280 285
Met Asp Met Ala Ala Ser Gly Ser Thr Gly Ala Ser Ser Ala Glu Ser
290 295 300
Asn Ser Trp Gly His Ile Ser Ser Leu Gly Tyr Pro Gln Leu Val Ser
305 310 315 320
Asp Tyr Glu Thr Cys Leu Gln Ser Met Pro Gln Asp Ser Ser Phe Glu
325 330 335
Asp Ser Ser Phe Phe Gly Pro Gln
340
<210>25
<211>537
<212>DNA
<213>截形苜蓿
<400>25
atgggaagag ctccttgctg tgacaaagcc aatgtcaaaa aaggtccatg gtcacctgaa 60
gaagatgcta aactcaagtc ttacatagaa caaaatggta ctggtggaaa ttggattgct 120
ctacctcaga agataggact taagagatgt gggaaaagct gtcgtcttag atggttaaat 180
tatcttagac caaatattaa acatggtggt ttctccgaag aagaagacga cattatttgc 240
agcctctatg ttagtatcgg aagcaggtgg tctatcatag cagcacaatt accaggacga 300
acggataatg atataaagaa ctactggaac actaggctga agaagaagct ccttgggaaa 360
caaaggaagg agcaacaagc tcgccgagtt agcaacatga aacaagagat gaaaagagaa 420
acaaatcaga atttgatgat ggcggctttg ggtgtcaata gtatgagttc agcaccatat 480
tggcctgcag agtattctgt tcatcctatg ccagtttcaa attcatccat aatttga 537
<210>26
<211>178
<212>PRT
<213>截形苜蓿
<400>26
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asp Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Gln Ala Arg
115 120 125
Arg Val Ser Asn Met Lys Gln Glu Met Lys Arg Glu Thr Asn Gln Asn
130 135 140
Leu Met Met Ala Ala Leu Gly Val Asn Ser Met Ser Ser Ala Pro Tyr
145 150 155 160
Trp Pro Ala Glu Tyr Ser Val His Pro Met Pro Val Ser Asn Ser Ser
165 170 175
Ile Ile
<210>27
<211>948
<212>DNA
<213>澳洲茄
<400>27
atgggaagag ctccatgttg tgataaagca aatgtaaaaa gaggtccatg gtctccagaa 60
gaagatgcaa aacttaaaga ttttattcat aaatttggta ctgctggtaa ttggattgca 120
cttcctcaaa aagcaggact aaggagatgt ggaaaaagtt gtagattgag atggctaaat 180
tatttaaggc ctaatataaa gcatggtgat ttttctgatg atgaagatag agttatttgt 240
aacttatatg ccaatattgg aagcaggtgg tcaattatag cggcgcaatt acctggaaga 300
acagacaatg atatcaaaaa ttattggaac acgaagctca agaaaaagct catgggatta 360
ataataccta ataacaattc ttcttcttct tataataata ataataataa ttaccaaaaa 420
aaattaccat attttccatc aacttctctt catcaagccc aacaaaatct tgggcctttt 480
attacaggcc cagaacagcc cattattatt ccagcagccc aacaaaacaa tttgttcacc 540
aataataaca acaacttgat gatgatagcc aataatttgc aaaattatcc aaattttggt 600
gctacaaatt acaatttgca aaattatcca aattttgggg aagttgcaag ttgttcttca 660
tcagatggaa gccaaatgag ttttggtact aataaagata ttattaatat taaaagagaa 720
gaaattatga gttttggtgg tcatcatgat ggtgctattt atgaagaaat taataataac 780
caacaattta attttggtta ttttggaaat actatgcagg agattgctac aagttgttgt 840
accaatggca atagtggtag tactaccaat agtagtaata attttttgtt gtacaataat 900
gatgaaaatt gtaacaagtc aaatgagata gggatgtttt attactaa 948
<210>28
<211>315
<212>PRT
<213>番茄
<400>28
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Phe
20 25 30
Gly Thr Ala Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp Asp Glu Asp Arg Val Ile Cys
65 70 75 80
Asn Leu Tyr Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Leu Ile Ile Pro Asn Asn Asn Ser Ser
115 120 125
Ser Ser Tyr Asn Asn Asn Asn Asn Asn Tyr Gln Lys Lys Leu Pro Tyr
130 135 140
Phe Pro Ser Thr Ser Leu His Gln Ala Gln Gln Asn Leu Gly Pro Phe
145 150 155 160
Ile Thr Gly Pro Glu Gln Pro Ile Ile Ile Pro Ala Ala Gln Gln Asn
165 170 175
Asn Leu Phe Thr Asn Asn Asn Asn Asn Leu Met Met Ile Ala Asn Asn
180 185 190
Leu Gln Asn Tyr Pro Asn Phe Gly Ala Thr Asn Tyr Asn Leu Gln Asn
195 200 205
Tyr Pro Asn Phe Gly Glu Val Ala Ser Cys Ser Ser Ser Asp Gly Ser
210 215 220
Gln Met Ser Phe Gly Thr Asn Lys Asp Ile Ile Asn Ile Lys Arg Glu
225 230 235 240
Glu Ile Met Ser Phe Gly Gly His His Asp Gly Ala Ile Tyr Glu Glu
245 250 255
Ile Asn Asn Asn Gln Gln Phe Asn Phe Gly Tyr Phe Gly Asn Thr Met
260 265 270
Gln Glu Ile Ala Thr Ser Cys Cys Thr Asn Gly Asn Ser Gly Ser Thr
275 280 285
Thr Asn Ser Ser Asn Asn Phe Leu Leu Tyr Asn Asn Asp Glu Asn Cys
290 295 300
Asn Lys Ser Asn Glu Ile Gly Met Phe Tyr Tyr
305 310 315
<210>29
<211>714
<212>DNA
<213>番茄
<400>29
atgggaagag ctccatgttg tgataaagca aatgtgaaga aagggccatg gtcaccagaa 60
gaagatgcaa aattaaaaga atatattgac aaatttggca ctggtggaaa ttggattgct 120
cttccacaaa aagctgggct aagaagatgt ggaaaaagct gtcgattaag atggttaaat 180
tatcttaggc caaatattaa acacggagag ttttcagacg aagaagacag aatcatttgc 240
agcctttatg ctaacattgg aagcaggtgg tcaatcatag cagctcaatt accaggcagg 300
acagataatg atatcaaaaa ctattggaac acgaagctga agaagaaatt aatgggattt 360
gtctcttcat ctcacaagat taggcctctt aatcaccatg attatcacca ccaaattccc 420
actaattgtt acaataatta ttcctcactt gttcaagctt catctttatt aatctcatca 480
aattatccca acaacacaac tttcccatgc tatgaaacaa atattcctag tacaacccca 540
tcaagtacaa gtttcttaag cgcgggtgca tctactagtt gtacctcagg cattactgct 600
agtactttcg cgggtcgtac tacctcttct gatgagaggt atgacatttc gaattttaat 660
tttcatagct atatgtataa taacaatggt ggttttagtg aaggagaaaa gtga 714
<210>30
<211>237
<212>PRT
<213>番茄
<400>30
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Asp Lys Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Phe Val Ser Ser Ser His Lys Ile Arg
115 120 125
Pro Leu Asn His His Asp Tyr His His Gln Ile Pro Thr Asn Cys Tyr
130 135 140
Asn Asn Tyr Ser Ser Leu Val Gln Ala Ser Ser Leu Leu Ile Ser Ser
145 150 155 160
Asn Tyr Pro Asn Asn Thr Thr Phe Pro Cys Tyr Glu Thr Asn Ile Pro
165 170 175
Ser Thr Thr Pro Ser Ser Thr Ser Phe Leu Ser Ala Gly Ala Ser Thr
180 185 190
Ser Cys Thr Ser Gly Ile Thr Ala Ser Thr Phe Ala Gly Arg Thr Thr
195 200 205
Ser Ser Asp Glu Arg Tyr Asp Ile Ser Asn Phe Asn Phe His Ser Tyr
210 215 220
Met Tyr Asn Asn Asn Gly Gly Phe Ser Glu Gly Glu Lys
225 230 235
<210>31
<211>918
<212>DNA
<213>拟南芥
<400>31
atgggaagag caccgtgctg cgacaagatg gcggtgaaga aagggccatg gtcgacggag 60
gaagatgccg tgcttaagtc ttacatcgaa aaacacggca ccggcaacaa ctggatttcc 120
ctccctcaga gaattgggat aaagaggtgc gggaagagtt gtcgtttgag atggcttaat 180
tacttgaggc ctaacttaaa gcatggaggc ttcaccgatg aagaagatta catcatttgc 240
agcctttaca ttactattgg aagcaggtgg tctatcattg cttcacaatt accgggaaga 300
acagacaacg acatcaaaaa ctattggaac acgaggctga agaagaagct attgagcaag 360
caagggaagg catttcatca acaacttaat gtcaaatttg agcgtggaac aacatcatca 420
tcatcgagtc agaaccagat ccaaatcttt catgatgaga acaccaaatc gaaccaaaca 480
ttatataatc aagtggtgga tccatcaatg agagcttttg ccatggaaga acaaagcatg 540
atcaagaatc agatattgga accattttct tgggaaccaa acaaggtttt gtttgatgtt 600
gattatgatg cagctgcttc atcttatcat catcatgcat ccccatcatt gaactccatg 660
agcagtacta gtagtattgg tactaataat tcatctttac aaatgtctca ctacaccgtc 720
aatcacaatg atcatgatca accagatatg ttctttatgg acgggtttga gaatttccag 780
gccgagctat ttgatgagat agccaacaac aacacggtag aaaatggctt tgacggaacc 840
gagatcctga tcaataacaa ctacttggat cacgatatta gctccttcat tgattatcct 900
ctatacgata atgagtag 918
<210>32
<211>305
<212>PRT
<213>拟南芥
<400>32
Met Gly Arg Ala Pro Cys Cys Asp Lys Met Ala Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Thr Glu Glu Asp Ala Val Leu Lys Ser Tyr Ile Glu Lys His
20 25 30
Gly Thr Gly Asn Asn Trp Ile Ser Leu Pro Gln Arg Ile Gly Ile Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Leu Lys His Gly Gly Phe Thr Asp Glu Glu Asp Tyr Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Ser Lys Gln Gly Lys Ala Phe His Gln Gln
115 120 125
Leu Asn Val Lys Phe Glu Arg Gly Thr Thr Ser Ser Ser Ser Ser Gln
130 135 140
Asn Gln Ile Gln Ile Phe His Asp Glu Asn Thr Lys Ser Asn Gln Thr
145 150 155 160
Leu Tyr Asn Gln Val Val Asp Pro Ser Met Arg Ala Phe Ala Met Glu
165 170 175
Glu Gln Ser Met Ile Lys Asn Gln Ile Leu Glu Pro Phe Ser Trp Glu
180 185 190
Pro Asn Lys Val Leu Phe Asp Val Asp Tyr Asp Ala Ala Ala Ser Ser
195 200 205
Tyr His His His Ala Ser Pro Ser Leu Asn Ser Met Ser Ser Thr Ser
210 215 220
Ser Ile Gly Thr Asn Asn Ser Ser Leu Gln Met Ser His Tyr Thr Val
225 230 235 240
Asn His Asn Asp His Asp Gln Pro Asp Met Phe Phe Met Asp Gly Phe
245 250 255
Glu Asn Phe Gln Ala Glu Leu Phe Asp Glu Ile Ala Asn Asn Asn Thr
260 265 270
Val Glu Asn Gly Phe Asp Gly Thr Glu Ile Leu Ile Asn Asn Asn Tyr
275 280 285
Leu Asp His Asp Ile Ser Ser Phe Ile Asp Tyr Pro Leu Tyr Asp Asn
290 295 300
Glu
305
<210>33
<211>990
<212>DNA
<213>拟南芥
<400>33
atgggaagag ctccgtgttg cgacaagaca aaagtgaagc gagggccttg gtcgcctgaa 60
gaagactcta aacttagaga ttacattgaa aagtatggta atggtggaaa ttggatctct 120
ttccccctca aagccggttt gaggagatgt gggaagagtt gtagactgag gtggctaaac 180
tatttgagac caaacataaa gcatggtgac ttctctgagg aagaagacag gatcattttt 240
agtctcttcg ctgccatagg aagcaggtgg tcaataatag cagctcatct accgggacga 300
acagacaacg acataaaaaa ctattggaac acaaagctaa ggaagaaact cttgtcttct 360
tcctctgatt catcatcatc agccatggct tctccttatc taaaccctat ttctcaggat 420
gtgaaaagac caacctcacc aacaacaatc ccatcttctt cttacaatcc gtatgctgaa 480
aaccctaatc aatacccaac aaaatccctc atctccagca tcaatggctt cgaagctggt 540
gacaaacaga taatttccta tattaaccct aattatcctc aagatctcta tctctcggac 600
agcaacaaca acacctcgaa cgcaaatggt ttcttgctca accacaatat gtgtgatcag 660
tacaagaacc acaccagttt ttcttcagac gtcaatggga taagatcaga gattatgatg 720
aagcaagaag agataatgat gatgatgatg atagaccacc acattgacca gaggacaaaa 780
gggtacaatg gggaattcac acaagggtat tataattact acaatgggca tggggatttg 840
aagcaaatga ttagtggaac aggcactaat tctaacataa acatgggtgg ttcaggttca 900
tcttctagtt cgataagcaa cctagctgag aacaaaagca gtggtagcct cctactagaa 960
tacaaatgct tgccctattt ctactcctag 990
<210>34
<211>329
<212>PRT
<213>拟南芥
<400>34
Met Gly Arg Ala Pro Cys Cys Asp Lys Thr Lys Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ser Lys Leu Arg Asp Tyr Ile Glu Lys Tyr
20 25 30
Gly Asn Gly Gly Asn Trp Ile Ser Phe Pro Leu Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Ile Ile Phe
65 70 75 80
Ser Leu Phe Ala Ala Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala His
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Arg Lys Lys Leu Leu Ser Ser Ser Ser Asp Ser Ser Ser Ser Ala
115 120 125
Met Ala Ser Pro Tyr Leu Asn Pro Ile Ser Gln Asp Val Lys Arg Pro
130 135 140
Thr Ser Pro Thr Thr Ile Pro Ser Ser Ser Tyr Asn Pro Tyr Ala Glu
145 150 155 160
Asn Pro Asn Gln Tyr Pro Thr Lys Ser Leu Ile Ser Ser Ile Asn Gly
165 170 175
Phe Glu Ala Gly Asp Lys Gln Ile Ile Ser Tyr Ile Asn Pro Asn Tyr
180 185 190
Pro Gln Asp Leu Tyr Leu Ser Asp Ser Asn Asn Asn Thr Ser Asn Ala
195 200 205
Asn Gly Phe Leu Leu Asn His Asn Met Cys Asp Gln Tyr Lys Asn His
210 215 220
Thr Ser Phe Ser Ser Asp Val Asn Gly Ile Arg Ser Glu Ile Met Met
225 230 235 240
Lys Gln Glu Glu Ile Met Met Met Met Met Ile Asp His His Ile Asp
245 250 255
Gln Arg Thr Lys Gly Tyr Asn Gly Glu Phe Thr Gln Gly Tyr Tyr Asn
260 265 270
Tyr Tyr Asn Gly His Gly Asp Leu Lys Gln Met Ile Ser Gly Thr Gly
275 280 285
Thr Asn Ser Asn Ile Asn Met Gly Gly Ser Gly Ser Ser Ser Ser Ser
290 295 300
Ile Ser Asn Leu Ala Glu Asn Lys Ser Ser Gly Ser Leu Leu Leu Glu
305 310 315 320
Tyr Lys Cys Leu Pro Tyr Phe Tyr Ser
325
<210>35
<211>897
<212>DNA
<213>拟南芥
<400>35
atgggtaggg ctccatgttg tgacaaggca aatgtgaaga gaggtccatg gtctcctgaa 60
gaagacgcaa agcttaaaga ttacatcgag aaacaaggaa ctggtggcaa ttggattgct 120
ctccctcaca aagctggttt aaggagatgt gggaagagtt gcagactgag atggttaaat 180
tatttgagac caaacataag acatggagat ttcactgaag aagaagacaa tattatctac 240
agcctctttg cctccattgg aagcaggtgg tcagtaatag cagctcactt gcaaggtaga 300
actgataatg acatcaagaa ctattggaac actaagctca agaagaagct catagccacc 360
atggctcctc ctccacatca ccacttagcc attgctacat catcatcatc agcatcccca 420
tcatcatcat cacattacaa catgatcaat agtcttcttc cgtataaccc atcaacaaac 480
caacttctca cacctcatca gggtatcatg atgacaatga tgggccaaca acaacaacta 540
ttttatcaag aagacatggg caatttggta aattctccaa acagaaacaa tctcataatg 600
agccatcaag aagacaacca agagcaaagt acaaacaagg gaataatgtt gttgagtgat 660
gtaagaagtg ggtcgagtac aacaagtaca gtaacaagag tgaagatgga acatcgtgat 720
catgatgatc atcatcatca tcatgaagaa gatgagagat caatgacctc ggtagtgatg 780
gaagattatg gaatggagga gatcaagcaa ttaataagta gtagttgtac gagtagtaac 840
aatagcttgt ggtttgacga aaacaagacg gaggataagt tcatgttgta ctactga 897
<210>36
<211>298
<212>PRT
<213>拟南芥
<400>36
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Gln
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Asp Phe Thr Glu Glu Glu Asp Asn Ile Ile Tyr
65 70 75 80
Ser Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala His
85 90 95
Leu Gln Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Ile Ala Thr Met Ala Pro Pro Pro His His His
115 120 125
Leu Ala Ile Ala Thr Ser Ser Ser Ser Ala Ser Pro Ser Ser Ser Ser
130 135 140
His Tyr Asn Met Ile Asn Ser Leu Leu Pro Tyr Asn Pro Ser Thr Asn
145 150 155 160
Gln Leu Leu Thr Pro His Gln Gly Ile Met Met Thr Met Met Gly Gln
165 170 175
Gln Gln Gln Leu Phe Tyr Gln Glu Asp Met Gly Asn Leu Val Asn Ser
180 185 190
Pro Asn Arg Asn Asn Leu Ile Met Ser His Gln Glu Asp Asn Gln Glu
195 200 205
Gln Ser Thr Asn Lys Gly Ile Met Leu Leu Ser Asp Val Arg Ser Gly
210 215 220
Ser Ser Thr Thr Ser Thr Val Thr Arg Val Lys Met Glu His Arg Asp
225 230 235 240
His Asp Asp His His His His His Glu Glu Asp Glu Arg Ser Met Thr
245 250 255
Ser Val Val Met Glu Asp Tyr Gly Met Glu Glu Ile Lys Gln Leu Ile
260 265 270
Ser Ser Ser Cys Thr Ser Ser Asn Asn Ser Leu Trp Phe Asp Glu Asn
275 280 285
Lys Thr Glu Asp Lys Phe Met Leu Tyr Tyr
290 295
<210>37
<211>622
<212>DNA
<213>拟斯卑尔托山羊草
<400>37
ggcgccgagc tgcaacaagg cgaccgtgaa gagggcgcca tgctcaccgg aggaggacgc 60
gatgctcaag gcctacattg aggagcgtgg caccggcaac aactggattg cactgccaca 120
caagattggg ctgaagagat gcggcaagag ctgcaggctg aggtggctca actacctgag 180
gcccaacata aagcactggg acttcacccc agaggaggac agcaccatct gcaagctcta 240
cattagcatc gggagcaggt ggtcaatcat cgccgcacag ctgccaggaa ggaccgacaa 300
cgacgtcaag aactactgga acaccaagct caagaagcgg ctccttggcg gccgccgcaa 360
ggaccgcggc gccggcacgc agcagcaccg ccagggagag ctggacggcg gcaacaacga 420
aggggagcag cagccgctga gcgcgtccgc gatggagagg atccagctct gcatgcagct 480
gcaggaaatg cagaaccccc tgagcagcat cggcaaccac aacaacccct tgcacctgtg 540
gcagcctgga agccatcatc aggtggccgc cactcacagt aacaacaggc acaacaacag 600
caacagcagc cgcagcagca gc 622
<210>38
<211>693
<212>DNA
<213>金鱼草
<400>38
atgggaagag ctccatgttg tgataaggca aatgtaaaaa aagggccatg gtcacctgaa 60
gaagatgcaa agcttaaaga gtacatagag caacaaggca gtgttggaaa ttggattgct 120
cttccacaaa aagctggttt gagaagatgt ggaaagagct gcagattgag atggttgaac 180
tatcttagac caaacattaa gcatggagag ttttcagatg atgaagatag aatcatttgc 240
agcctctttg ctaacattgg aagcaggtgg tcaataatag cagctcaatt accaggaaga 300
actgataatg acatcaagaa ctattggaac acaaaactca agaaaaaact catgggagca 360
atgtcccctt catatcagaa aaaacctcat caagctacaa cttttccttt accatctact 420
cataattttc tctcacaaga ctctccatat tcatctcttc tctcacaaat ctcatcagca 480
tatgaaggta acaacaacaa ctattacaac acctcaatta aggccttctc aagagcctat 540
gaacccataa tttctccaaa tcctaatgcc cctaattcag tcctacaaac acaagattca 600
tatgtgggtc ccatgcatgg cgattacaat aataataatc tcctaatctt tggaggggaa 660
ccaagctgca gttcatctga tgggagtcaa att 693
<210>39
<211>231
<212>PRT
<213>金鱼草
<400>39
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Glu Gln Gln
20 25 30
Gly Ser Val Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Phe Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Ala Met Ser Pro Ser Tyr Gln Lys Lys
115 120 125
Pro His Gln Ala Thr Thr Phe Pro Leu Pro Ser Thr His Asn Phe Leu
130 135 140
Ser Gln Asp Ser Pro Tyr Ser Ser Leu Leu Ser Gln Ile Ser Ser Ala
145 150 155 160
Tyr Glu Gly Asn Asn Asn Asn Tyr Tyr Asn Thr Ser Ile Lys Ala Phe
165 170 175
Ser Arg Ala Tyr Glu Pro Ile Ile Ser Pro Asn Pro Asn Ala Pro Asn
180 185 190
Ser Val Leu Gln Thr Gln Asp Ser Tyr Val Gly Pro Met His Gly Asp
195 200 205
Tyr Asn Asn Asn Asn Leu Leu Ile Phe Gly Gly Glu Pro Ser Cys Ser
210 215 220
Ser Ser Asp Gly Ser Gln Ile
225 230
<210>40
<211>1050
<212>DNA
<213>耧斗菜
<400>40
atgggaagag ctccttgttg tgacaaagcc aatgttaaga aaggtccatg gtcacctgaa 60
gaagatgcaa aactcaaaga atacattgaa caacatggta caggagggaa ttggatcgcc 120
ttgccacaaa agattggcct taagagatgt gggaagagct gtcgtctcag atggttgaat 180
tatctccgtc caaatcttaa gcatggaggt ttctctgaag aagaagatca catgatttgc 240
agcctctata tgagtattgg aagccgatgg tccatcattg cagcacaatt acctggtcgg 300
actgacaacg acataaaaaa ctattggaac actaagctga agaagaagct cttgggaaaa 360
caaaggaagg aacaccaagc tcgtagagct agctacctaa agcaagacag catgaagaaa 420
agcaattcta ttccactggt aattgcagat catgggatgc aaacgccgta ctggccacca 480
gagccactga tgcctatatc aacaccgtat ccaaaccaag gcaatcggca taatgatcat 540
gcatctctta gaaaattact gatcaaactt ggggggaagt tttctgatga ttatcaacaa 600
gaacccaaca acgatgcaaa gaataatctt cacctcccca ttgaatgttc ctcaacatat 660
caacaattca atgatcagaa atccattaat ctattctctt cttcgtcttc cataagttcc 720
ttggatattc cctattctaa attgttgacc aacgagtaca atatcgaggg agcaagcatg 780
cacatgttgc aaggccttaa cggatttccg gttgagtttg atgagatgat atgtagcaat 840
ccagagagtt tagatgaatt ggaatgccat tatggagttg gtatggacaa tggagtaagc 900
agtagtggtc caatatcaac agaaagcact acctgggatg acatgggatc tttcatcaac 960
cctcctaata tcaccaacta tgaagcttta caacaagaag tgatacaaga atgtgcatta 1020
ttcgaccagt cgaggtacct tggatttcag 1050
<210>41
<211>350
<212>PRT
<213>耧斗菜
<400>41
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Leu Lys His Gly Gly Phe Ser Glu Glu Glu Asp His Met Ile Cys
65 70 75 80
Ser Leu Tyr Met Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu His Gln Ala Arg
115 120 125
Arg Ala Ser Tyr Leu Lys Gln Asp Ser Met Lys Lys Ser Asn Ser Ile
130 135 140
Pro Leu Val Ile Ala Asp His Gly Met Gln Thr Pro Tyr Trp Pro Pro
145 150 155 160
Glu Pro Leu Met Pro Ile Ser Thr Pro Tyr Pro Asn Gln Gly Asn Arg
165 170 175
His Asn Asp His Ala Ser Leu Arg Lys Leu Leu Ile Lys Leu Gly Gly
180 185 190
Lys Phe Ser Asp Asp Tyr Gln Gln Glu Pro Asn Asn Asp Ala Lys Asn
195 200 205
Asn Leu His Leu Pro Ile Glu Cys Ser Ser Thr Tyr Gln Gln Phe Asn
210 215 220
Asp Gln Lys Ser Ile Asn Leu Phe Ser Ser Ser Ser Ser Ile Ser Ser
225 230 235 240
Leu Asp Ile Pro Tyr Ser Lys Leu Leu Thr Asn Glu Tyr Asn Ile Glu
245 250 255
Gly Ala Ser Met His Met Leu Gln Gly Leu Asn Gly Phe Pro Val Glu
260 265 270
Phe Asp Glu Met Ile Cys Ser Asn Pro Glu Ser Leu Asp Glu Leu Glu
275 280 285
Cys His Tyr Gly Val Gly Met Asp Asn Gly Val Ser Ser Ser Gly Pro
290 295 300
Ile Ser Thr Glu Ser Thr Thr Trp Asp Asp Met Gly Ser Phe Ile Asn
305 310 315 320
Pro Pro Asn Ile Thr Asn Tyr Glu Ala Leu Gln Gln Glu Val Ile Gln
325 330 335
Glu Cys Ala Leu Phe Asp Gln Ser Arg Tyr Leu Gly Phe Gln
340 345 350
<210>42
<211>1005
<212>DNA
<213>耧斗菜
<400>42
atgggaaggg caccttgttg tgacaaagca aatgtcaaga aaggaccatg gtctcctgaa 60
gaagacacaa ggcttaagga gtacatagag aaatttggga ctggtgggaa ctggattgct 120
cttccacaaa aagctggtct aaagagatgt gggaagagtt gtagattgag atggcttaat 180
tatcttaggc caaacataaa acatggccaa ttctccgatg atgaagataa agtgatctgc 240
agcctctttg ctagcatagg tagcaggtgg tcaataatag ctgcacagtt gccaggcagg 300
actgacaatg atatcaaaaa ctattggaac accaagctca agaaaaaatt tatggggttt 360
gttccttcat cacagattaa agcacttcca ccaatctttc catctccatt ccacactgga 420
tcaacatatg attactaccc tttatcaaaa tctcttccag accttgaatc tctttcaatc 480
ccatcaaatt ttttgaacaa cactaacact acaaccagta ttattagtac atctcttcat 540
gaatcccaac aaaatttgga aggtttcatg caccattatc aagagaaaga caactttctt 600
atctttggag gtgaacctag ttgcagttct tctgatggaa gctgtactaa tcaaataagc 660
tacaacaaag atattgagta tgactatagt ggcaatggca atggtggtgg tggtggtgga 720
aatggtccta atggtgtaag attaggccaa gagagttatt tttgcaatgg agttcaagat 780
aatcagaaat tcatgcttgg taatggttcc attggtagta atggatggtc tgatcagagg 840
ctaaatagtg gattaatgtg gggtgatgat cagacatcat taccattaga tcatcatcat 900
tatgatggtc ttgaggatat taaactagtc aaaaacagta gtggttcttg tagtaatatt 960
ttcaatggtg atgaaactaa agcacagagt agaatcatgt acttc 1005
<210>43
<211>335
<212>PRT
<213>耧斗菜
<400>43
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Arg Leu Lys Glu Tyr Ile Glu Lys Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gln Phe Ser Asp Asp Glu Asp Lys Val Ile Cys
65 70 75 80
Ser Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Phe Met Gly Phe Val Pro Ser Ser Gln Ile Lys Ala
115 120 125
Leu Pro Pro Ile Phe Pro Ser Pro Phe His Thr Gly Ser Thr Tyr Asp
130 135 140
Tyr Tyr Pro Leu Ser Lys Ser Leu Pro Asp Leu Glu Ser Leu Ser Ile
145 150 155 160
Pro Ser Asn Phe Leu Asn Asn Thr Asn Thr Thr Thr Ser Ile Ile Ser
165 170 175
Thr Ser Leu His Glu Ser Gln Gln Asn Leu Glu Gly Phe Met His His
180 185 190
Tyr Gln Glu Lys Asp Asn Phe Leu Ile Phe Gly Gly Glu Pro Ser Cys
195 200 205
Ser Ser Ser Asp Gly Ser Cys Thr Asn Gln Ile Ser Tyr Asn Lys Asp
210 215 220
Ile Glu Tyr Asp Tyr Ser Gly Asn Gly Asn Gly Gly Gly Gly Gly Gly
225 230 235 240
Asn Gly Pro Asn Gly Val Arg Leu Gly Gln Glu Ser Tyr Phe Cys Asn
245 250 255
Gly Val Gln Asp Asn Gln Lys Phe Met Leu Gly Asn Gly Ser Ile Gly
260 265 270
Ser Asn Gly Trp Ser Asp Gln Arg Leu Asn Ser Gly Leu Met Trp Gly
275 280 285
Asp Asp Gln Thr Ser Leu Pro Leu Asp His His His Tyr Asp Gly Leu
290 295 300
Glu Asp Ile Lys Leu Val Lys Asn Ser Ser Gly Ser Cys Ser Asn Ile
305 310 315 320
Phe Asn Gly Asp Glu Thr Lys Ala Gln Ser Arg Ile Met Tyr Phe
325 330 335
<210>44
<211>435
<212>DNA
<213>花生
<220>
<221>其他特征
<222>(335)..(335)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(340)..(340)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(377)..(377)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(389)..(389)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(414)..(414)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(424)..(424)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(433)..(433)
<223>n可以是任意核苷
<400>44
atgggaagag ctccttgttg tgacaaagca aatgtgaaga gaggaccatg gtctcctgat 60
gaggatgcaa cactcaagaa ctatcttcac actcatggca ctggaggaaa ttggattgca 120
ttgccaagaa aagctggttt aaggaggtgt gggaagagtt gccgtctaag gtggctgaat 180
tatctaaggc cagatataaa acatggagga tttaccgaac aagaggatca aatcatttgc 240
actctctata ctcaaatggg aagcagatgg tctgcaatag catctcaact tcctggcaga 300
acagacaatg atgtcaaaaa ctattggaac accangctcn agaagaagct aatggcagga 360
aaagtaattt cccctantaa taataatant aataaaacat tattgactac tcancgaaac 420
tcangatttt canga 435
<210>45
<211>145
<212>PRT
<213>花生
<220>
<221>其他特征
<222>(112)..(112)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(114)..(114)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(126)..(126)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(130)..(130)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(138)..(138)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(142)..(142)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(145)..(145)
<223>X可以是任意氨基酸
<400>45
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ala Thr Leu Lys Asn Tyr Leu His Thr His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Arg Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Glu Gln Glu Asp Gln Ile Ile Cys
65 70 75 80
Thr Leu Tyr Thr Gln Met Gly Ser Arg Trp Ser Ala Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Xaa
100 105 110
Leu Xaa Lys Lys Leu Met Ala Gly Lys Val Ile Ser Pro Xaa Asn Asn
115 120 125
Asn Xaa Asn Lys Thr Leu Leu Thr Thr Xaa Arg Asn Ser Xaa Phe Ser
130 135 140
Xaa
145
<210>46
<211>476
<212>DNA
<213>花生
<400>46
atgggaagag ctccttgttg tgacaaagca aatgtgaaga gaggaccatg gtctcctgat 60
gaggatgcaa cactcaagaa ctatcttcac actcatggca ctggaggaaa ttggattgca 120
ttgccaagaa aagctggttt aaggaggtgt gggaagagtt gccgtctaag gtggctgaat 180
tatctaaggc cagatataaa acatggagga tttaccgaac aagaggatca aatcatttgc 240
actctctata ctcaaatggg aagcagatgg tctgcaatag catctcaact tcctggcaga 300
acagacaatg atgtcaaaaa ctattggaac accaagctca agaagaagct aatggcagga 360
aaagtaattt cccctaataa taataataat aataaaacat tattgactac tcaacaaaac 420
tcagattttc aagataataa aaattgttca ccaccctcat catcatcatc attagt 476
<210>47
<211>159
<212>PRT
<213>花生
<400>47
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ala Thr Leu Lys Asn Tyr Leu His Thr His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Arg Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Glu Gln Glu Asp Gln Ile Ile Cys
65 70 75 80
Thr Leu Tyr Thr Gln Met Gly Ser Arg Trp Ser Ala Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Ala Gly Lys Val Ile Ser Pro Asn Asn Asn
115 120 125
Asn Asn Asn Lys Thr Leu Leu Thr Thr Gln Gln Asn Ser Asp Phe Gln
130 135 140
Asp Asn Lys Asn Cys Ser Pro Pro Ser Ser Ser Ser Ser Leu Val
145 150 155
<210>48
<211>744
<212>DNA
<213>花生属野生种
<400>48
atgggaagag ctccatgttg tgacaaggca aatgtgaaga gaggaccatg gtcacctgaa 60
gaagacttaa agctcaaaga atacatacac aagcatggca ctggtggcaa ttggattgct 120
cttcctcaaa aagctggtct taagagatgt gggaagagtt gcagactgag atggcttaac 180
tatctgaggc caaacattaa gcatggagaa ttttctgaag aggaagacag aatcatttgc 240
agcctctatg ttaacattgg aagcagatgg tcaattatag cagctcagtt gccaggaagg 300
actgacaatg atataaagaa ttattggaac actaagctca agaaaaaact catctctggt 360
ttcattccca attcttcttc tattcgacaa agaaaacatc atcatcatca acaacaacaa 420
caacctccat ttccatcatc atcaataatg tacatggatc aatgttatgg atcttatgtc 480
acaggccttg ttgatcctat ttcacttcct tcaactgaat actatgcaaa cacaacaaca 540
acaacctcag ttccatttta ccagcaccaa gtagattcca tagttagccc cagcagcatg 600
caacaaaatt atcacatgtt tggaagtgaa ggtagttgca gtttctctga taatggaagc 660
agtatcaagc aagaggaaat tgggtatcat catcaaggat catacaacaa cattattgca 720
tttgatgagt tcaacaacac acaa 744
<210>49
<211>248
<212>PRT
<213>花生属野生种
<400>49
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Leu Lys Leu Lys Glu Tyr Ile His Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Glu Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Asn Ile Gly Ser Arg Trp Ser Ilc Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Ile Ser Gly Phe Ile Pro Asn Ser Ser Ser Ile
115 120 125
Arg Gln Arg Lys His His His His Gln Gln Gln Gln Gln Pro Pro Phe
130 135 140
Pro Ser Ser Ser Ile Met Tyr Met Asp Gln Cys Tyr Gly Ser Tyr Val
145 150 155 160
Thr Gly Leu Val Asp Pro Ile Ser Leu Pro Ser Thr Glu Tyr Tyr Ala
165 170 175
Asn Thr Thr Thr Thr Thr Ser Val Pro Phe Tyr Gln His Gln Val Asp
180 185 190
Ser Ile Val Ser Pro Ser Ser Met Gln Gln Asn Tyr His Met Phe Gly
195 200 205
Ser Glu Gly Ser Cys Ser Phe Ser Asp Asn Gly Ser Ser Ile Lys Gln
210 215 220
Glu Glu Ile Gly Tyr His His Gln Gly Ser Tyr Asn Asn Ile Ile Ala
225 230 235 240
Phe Asp Glu Phe Asn Asn Thr Gln
245
<210>50
<211>477
<212>DNA
<213>二穗短柄草
<400>50
atggggcgcg cgccgtgctg cgacaaggcg agcgtgaagc gcgggccgtg gtcgccggag 60
gaggacgagc agctccggcg ctacgtccgc gcccatggca tcggcggcaa ctggatcgcc 120
ctgccgcaca aagccgggct gaagcggtgc ggcaagagct gccggctgcg gtggctgaac 180
tacctgcggc cggacatcag gcacggcggg tacacggccg aggaggaccg ggtcatctgc 240
tccctatacg ggtcaatcgg cagccggtgg tcgattatcg cgtccaagct ccccggccgc 300
acggacaacg acgtcaagaa ctactggaac accaagctca agaagaaggc cgtcgccatg 360
gggatgcagc atgccaccgg aggatcagcc ttctccgctc cccatagcca gtgcgcgctc 420
tcgccggcgg cctcgggctc ctcctcgtcg accgtcgaca ccactagctc cagcgcc 477
<210>51
<211>159
<212>PRT
<213>二穗短柄草
<400>51
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Gln Leu Arg Arg Tyr Val Arg Ala His
20 25 30
Gly Ile Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Tyr Thr Ala Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Gly Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ala Val Ala Met Gly Met Gln His Ala Thr Gly Gly
115 120 125
Ser Ala Phe Ser Ala Pro His Ser Gln Cys Ala Leu Ser Pro Ala Ala
130 135 140
Ser Gly Ser Ser Ser Ser Thr Val Asp Thr Thr Ser Ser Ser Ala
145 150 155
<210>52
<211>717
<212>DNA
<213>二穗短柄草
<400>52
atggggaggg cgccgtgctg cgacagggcg gcggtgaagc gtgggccgtg gtcgccggag 60
gaggacgaca agctgcgcga ctacatccag cgccacggca ccggcggcag ctggatcacc 120
ttccccaaga aagccgggct gaggaggtgt ggcaagagct gcaggctgcg gtggctcaac 180
tacctccgcc cggacatccg gcacggcggc ttcaccgacg aggaggacgc gctcatcttc 240
tccctctaca gcaagctcgg cagcaagtgg tcgctgatcg cgtcgcagct ggagaggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agaagcgcct cgcagccttc 360
tcctctcccc cgtcgtcttc ctcctccttc ctgccggcgc ctgcgcccat ggccgtggcc 420
gtcgcgcacc cgctcgccct ggccgtgccg accgtcaagg ccgagacgta cgcctacgac 480
gacttcatgg cgccgccggc cgcgctccac gtcttcgacc acccgttcgg caacggcgcc 540
gatcagcccg gctccacgac gtccgcctcc gcagcgtcgt ccatgtccaa ctggtcgtcc 600
gcggcggaca acgcgggggc cgccgacggg ttcttcgcgg acttctgcaa cgccggcgcg 660
gcggaccagt tcctcggcgg cttctactac cctctcgatc ccaccttgtc gctagtc 717
<210>53
<211>239
<212>PRT
<213>二穗短柄草
<400>53
Met Gly Arg Ala Pro Cys Cys Asp Arg Ala Ala Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Asp Lys Leu Arg Asp Tyr Ile Gln Arg His
20 25 30
Gly Thr Gly Gly Ser Trp Ile Thr Phe Pro Lys Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Asp Glu Glu Asp Ala Leu Ile Phe
65 70 75 80
Ser Leu Tyr Ser Lys Leu Gly Ser Lys Trp Ser Leu Ile Ala Ser Gln
85 90 95
Leu Glu Arg Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Ala Ala Phe Ser Ser Pro Pro Ser Ser Ser Ser
115 120 125
Ser Phe Leu Pro Ala Pro Ala Pro Met Ala Val Ala Val Ala His Pro
130 135 140
Leu Ala Leu Ala Val Pro Thr Val Lys Ala Glu Thr Tyr Ala Tyr Asp
145 150 155 160
Asp Phe Met Ala Pro Pro Ala Ala Leu His Val Phe Asp His Pro Phe
165 170 175
Gly Asn Gly Ala Asp Gln Pro Gly Ser Thr Thr Ser Ala Ser Ala Ala
180 185 190
Ser Ser Met Ser Asn Trp Ser Ser Ala Ala Asp Asn Ala Gly Ala Ala
195 200 205
Asp Gly Phe Phe Ala Asp Phe Cys Asn Ala Gly Ala Ala Asp Gln Phe
210 215 220
Leu Gly Gly Phe Tyr Tyr Pro Leu Asp Pro Thr Leu Ser Leu Val
225 230 235
<210>54
<211>1092
<212>DNA
<213>甘蓝型油菜
<400>54
atgggaaggg caccgtgttg tgacaaagcc aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcca aactcaaaga ttacatcgag aatagtggta caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcagactaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aggaagacac catcatttgt 240
aacctttatg ttactattgg tagcaggtgg tctataattg ctgcacaact gccgggaaga 300
acggacaacg atatcaagaa ctattggaac acgaggctaa agaagaagct tctaaacaaa 360
caaaggacag agttccaaga agctcggatg aagcaagaga tggtgatgat gaagagacaa 420
caacaaggac atgaccacat caatggtagt acggatcttt atctgaaaaa catgtttgga 480
tcatcaccat ggccattact acaacagctt cctcatcatc aagtacctct tgtgatgatg 540
gaaccaacaa gttgtaacta ctaccaaacg tcaccctctt gtaacctaga acaaaagcca 600
cttatcactt tcaataacat ggtcaagatt gaagaagaac cggagaaaac aaaccctgat 660
catcctcagc atcaaaattc tatcacaaac ccttttgatg tctccgtctc ccagctcttg 720
ttagatcctg attactactt aggatcagga ggaggagaag gggattttgc tatcatgagt 780
agcagcacaa attctccatt accaaacaca agtggtgatc aaaatgaaca tcagcagcaa 840
gagattcttc aatggtttgg gagtagtaat cttcagacag aagcaagcag tgatatgttc 900
ttaaacaaca tagggaatct tgagaccaac gaggacacaa gattctactc atcattagcc 960
ggtgctggag cggctccggc aggaggaacg acgagcacat cggcagatca aagcacaata 1020
agttgggagg acataacctc tcttgttaat tccgaagatg caagttactt caatggggca 1080
aatcatttgt aa 1092
<210>55
<211>363
<212>PRT
<213>甘蓝型油菜
<400>55
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Thr Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Thr Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Gly His
130 135 140
Asp His Ile Asn Gly Ser Thr Asp Leu Tyr Leu Lys Asn Met Phe Gly
145 150 155 160
Ser Ser Pro Trp Pro Leu Leu Gln Gln Leu Pro His His Gln Val Pro
165 170 175
Leu Val Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr Gln Thr Ser Pro
180 185 190
Ser Cys Asn Leu Glu Gln Lys Pro Leu Ile Thr Phe Asn Asn Met Val
195 200 205
Lys Ile Glu Glu Glu Pro Glu Lys Thr Asn Pro Asp His Pro Gln His
210 215 220
Gln Asn Ser Ile Thr Asn Pro Phe Asp Val Ser Val Ser Gln Leu Leu
225 230 235 240
Leu Asp Pro Asp Tyr Tyr Leu Gly Ser Gly Gly Gly Glu Gly Asp Phe
245 250 255
Ala Ile Met Ser Ser Ser Thr Asn Ser Pro Leu Pro Asn Thr Ser Gly
260 265 270
Asp Gln Asn Glu His Gln Gln Gln Glu Ile Leu Gln Trp Phe Gly Ser
275 280 285
Ser Asn Leu Gln Thr Glu Ala Ser Ser Asp Met Phe Leu Asn Asn Ile
290 295 300
Gly Asn Leu Glu Thr Asn Glu Asp Thr Arg Phe Tyr Ser Ser Leu Ala
305 310 315 320
Gly Ala Gly Ala Ala Pro Ala Gly Gly Thr Thr Ser Thr Ser Ala Asp
325 330 335
Gln Ser Thr Ile Ser Trp Glu Asp Ile Thr Ser Leu Val Asn Ser Glu
340 345 350
Asp Ala Ser Tyr Phe Asn Gly Ala Asn His Leu
355 360
<210>56
<211>1116
<212>DNA
<213>甘蓝型油菜
<400>56
atgggaagag caccgtgttg tgataaggct aacgtgaaga aagggccttg gtctcctgaa 60
gaagatgcaa agctcaaaga ttacatcgag agtagtggca caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcagactaag gtggcttaac 180
tatttgagac ctaacatcaa acatggtggc ttctctgagg aagaggacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaatt gcctggaaga 300
acagacaacg atatcaagaa ctattggaac acgaggctga agaagaagct tcttaacaaa 360
caaagaaaag agttccaaga agctcggatg aagcaagaga tggtgatgat gaaacgacag 420
caacaaggac aaggacaatg ccaaagtaat ggtagtacgg atctttatct gaacaacatg 480
tttagatcat caccatggcc attactgcct catcttcctc ctcctcatag tcaagtacct 540
cttgtgatga tggaaccaac aagctgcaat tactaccaac cgacaccgtc ttgcgcatta 600
gaacaaaagc cattgatccc actcaagaac atggtcaaga ttgaagcaga accggagaga 660
tcaaaccctg atcatcatta tccggaagac tcaatgacaa actcttttga tctttccttc 720
tctcagcttt tgttagatcc taattactac ctggaatcag gaggaggaga aggagagttt 780
gctctcatga gtagaagtac gaactctcca ttaccaaaca caagtagtga tcaccatcaa 840
catcaaaaag agattactca atggtttggg agtagtaatt ttcagacaga agctatcaat 900
gatgtgttct taaacaacaa caacttagcg aattttgaga ccaacgacga gaacgcaaaa 960
ctatatgcaa actcatcagt agccggagct ggagcagcgt ttgccggagg aacgacgagt 1020
acatcggctg atcaaagcac aataagttgg gaggacataa cctctcttgt taactccgat 1080
gatgcaagat acttcaatgg gccaaataat gtgtaa 1116
<210>57
<211>371
<212>PRT
<213>甘蓝型油菜
<400>57
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Ser Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Gly Gln
130 135 140
Gly Gln Cys Gln Ser Asn Gly Ser Thr Asp Leu Tyr Leu Asn Asn Met
145 150 155 160
Phe Arg Ser Ser Pro Trp Pro Leu Leu Pro His Leu Pro Pro Pro His
165 170 175
Ser Gln Val Pro Leu Val Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr
180 185 190
Gln Pro Thr Pro Ser Cys Ala Leu Glu Gln Lys Pro Leu Ile Pro Leu
195 200 205
Lys Asn Met Val Lys Ile Glu Ala Glu Pro Glu Arg Ser Asn Pro Asp
210 215 220
His His Tyr Pro Glu Asp Ser Met Thr Asn Ser Phe Asp Leu Ser Phe
225 230 235 240
Ser Gln Leu Leu Leu Asp Pro Asn Tyr Tyr Leu Glu Ser Gly Gly Gly
245 250 255
Glu Gly Glu Phe Ala Leu Met Ser Arg Ser Thr Asn Ser Pro Leu Pro
260 265 270
Asn Thr Ser Ser Asp His His Gln His Gln Lys Glu Ile Thr Gln Trp
275 280 285
Phe Gly Ser Ser Asn Phe Gln Thr Glu Ala Ile Asn Asp Val Phe Leu
290 295 300
Asn Asn Asn Asn Leu Ala Asn Phe Glu Thr Asn Asp Glu Asn Ala Lys
305 310 315 320
Leu Tyr Ala Asn Ser Ser Val Ala Gly Ala Gly Ala Ala Phe Ala Gly
325 330 335
Gly Thr Thr Ser Thr Ser Ala Asp Gln Ser Thr Ile Ser Trp Glu Asp
340 345 350
Ile Thr Ser Leu Val Asn Ser Asp Asp Ala Arg Tyr Phe Asn Gly Pro
355 360 365
Asn Asn Val
370
<210>58
<211>1454
<212>DNA
<213>甘蓝型油菜
<400>58
ctcctcttca tcatcaacat agttgagaaa gataaaaaat aaagagggag agaaagagaa 60
agaaacacat caagaacaag aataacgaat caagaagatg ggaagagcac cgtgttgtga 120
caaggctaac gtgaagaaag ggccgtggtc tcctgaagaa gacgcaaaac tcaaagatta 180
catcgagaat aatggcacag gaggcaactg gatcgcgttg cctcagaaga ttggtctaag 240
aagatgtggg aagagttgca gactaaggtg gctcaactat ttgagaccaa acatcaaaca 300
tggtggcttc tctgaggaag aggacaacat catttgtaac ctctatgtta ccattggtag 360
caggtggtct ataattgctg cacaattgcc tggaagaaca gacaacgata tcaagaacta 420
ttggaacacg aggctgaaga agaagcttct taacaaacaa agaaaagagt accaagaagc 480
tcggatgaag caagagatgg tgatgatgaa agcgacaagc aacaagggac aatgccaaag 540
taatgctagt acggatcttt attctgaaca acatgtttgg atcatcacca tggccattac 600
tgcctcagct tcctcctcct cattgtcaag tacctcttgt gatgatggaa ccaacaagct 660
gcaattacta ccaaccgaca ccgtcttgcg cattagaaca aaagccattg atcccactca 720
agaacatggt caagattgaa gaagaaccgg agagatcaaa ccctgatcat cattatccgg 780
aagactcaat gacaaactct tttgatcttt ccttttctca gcttttgtta gatcctaatt 840
actacctgga atcaggagga ggagaaggag agtttgctct catgagtagc agtacgaact 900
ctccattacc aaacacaagt gatgatcacc atcaacatca aaaagagatt actcaatggt 960
ttgggagtgg taattttcaa acagaagcta tcaatgatgt gttcttaaac aacaacaact 1020
tagcgaattt tgagaccaac gatgagaacg caaaactata tgcaaactca tcagtaaccg 1080
gagctggagc agcgtttgcc ggaggaacga cgagtacatc ggctgatcaa agcacaataa 1140
gttgggagga cataacctct cttcttaaat ccgatgatgc aagttacttc aatgggccaa 1200
atcatgtgta attttttctg caaaatttta tttttactta tatatataaa gaggtttttg 1260
tataagtata tatgacataa gagagtggta aaaaaaatac atggattgag attaatattc 1320
tcatatttgt gtattttagc ttcaacttag ctttattcac gaagagcaat atatatgatc 1380
ttttgagttt ttctttttga ttaatgcttt tataaatttg aaagagaaaa aaaaaaaaaa 1440
aaaaaaaaaa aaaa 1454
<210>59
<211>753
<212>DNA
<213>甘蓝型油菜
<400>59
atgggaaggg caccgtgttg tgacaaagcc aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcca aactcaaaga ttacatcgag aatagtggta caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcagactaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aggaagacac catcatttgt 240
aacctttatg ttactattgg tagcaggtgg tctataattg ctgcacaact gccgggaaga 300
acggacaacg atatcaagaa ctattggaac acgaggctaa agaagaagct tctaaacaaa 360
caaaggacag agttccaaga agctcggatg aagcaagaga tggtgatgat gaagagacaa 420
caacaaggac atgaccacat caatggtagt acggatcttt atctgaaaaa catgtttgga 480
tcatcaccat ggccattact acaacagctt cctcatcatc aagtacctct tgtgatgatg 540
gaaccaacaa gttgtaacta ctaccaaacg tcaccctctt gtaacctaga acaaaagcca 600
cttatcactt tcaataacat ggtcaagatt gaagaagaac cggagaaaac aaaccctgat 660
catcctcagc atcaaaattc tatcacaaac ccttttgatg tctccgtctc ccagctcttg 720
ttagatcctg attactactt aggatcagga gga 753
<210>60
<211>251
<212>PRT
<213>甘蓝型油菜
<400>60
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Thr Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Thr Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Gly His
130 135 140
Asp His Ile Asn Gly Ser Thr Asp Leu Tyr Leu Lys Asn Met Phe Gly
145 150 155 160
Ser Ser Pro Trp Pro Leu Leu Gln Gln Leu Pro His His Gln Val Pro
165 170 175
Leu Val Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr Gln Thr Ser Pro
180 185 190
Ser Cys Asn Leu Glu Gln Lys Pro Leu Ile Thr Phe Asn Asn Met Val
195 200 205
Lys Ile Glu Glu Glu Pro Glu Lys Thr Asn Pro Asp His Pro Gln His
210 215 220
Gln Asn Ser Ile Thr Asn Pro Phe Asp Val Ser Val Ser Gln Leu Leu
225 230 235 240
Leu Asp Pro Asp Tyr Tyr Leu Gly Ser Gly Gly
245 250
<210>61
<211>864
<212>DNA
<213>甘蓝型油菜
<400>61
atgggaaggg ctccatgttg tgacaaagca aacgtcaaga gaggtccatg gtctcctgaa 60
gaagatgcaa agcttaaaga ttacatagag aaacaaggca ctggtggcaa ctggattgct 120
ctccctcaca aagctggttt aagaagatgt gggaagagtt gtagactgag gtggttaaac 180
tatttgaggc caaacataag acatggagat ttctctgagg aagaagacaa gattatcttc 240
agcctctttt cctccattgg aagcaggtgg tcagtaattg cagctcacct gcatggtaga 300
actgataacg acatcaagaa ctattggagc actaagctca agaagaagct cattgccact 360
atggctcctc caccacctca tcatctctta gccattgcct catcatcatc atcaccatca 420
tcatcacact acaacatgac caatagtctt cctccgtata acccatcaat atctacaaat 480
gagctgttaa cacctcatca ggagatgatg atgacaatga tggaccaaca acaacaacaa 540
ctattatacc aagaagccgt ggacagtttg gtaaattctc caaatagcaa caagcttata 600
atgagccatc aagaagacag ccgggagcaa agtacaaaca aaggaataat gttgttgagt 660
gatgtaagaa gtgggtcaag tacaacaagt acagtaacaa gagtgaagat ggaacaacat 720
gatcatcatc atgaagagag atcaatggaa gattatggaa tggaggagat caatcactta 780
ataaatagta gttgtacgag tagtagtagc aacagcttgt ggtttgatga aaacaagacc 840
gatgatacgt tcatgttgta ctat 864
<210>62
<211>288
<212>PRT
<213>甘蓝型油菜
<400>62
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Gln
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Asp Phe Ser Glu Glu Glu Asp Lys Ile Ile Phe
65 70 75 80
Ser Leu Phe Ser Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala His
85 90 95
Leu His Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Ser Thr Lys
100 105 110
Leu Lys Lys Lys Leu Ile Ala Thr Met Ala Pro Pro Pro Pro His His
115 120 125
Leu Leu Ala Ile Ala Ser Ser Ser Ser Ser Pro Ser Ser Ser His Tyr
130 135 140
Asn Met Thr Asn Ser Leu Pro Pro Tyr Asn Pro Ser Ile Ser Thr Asn
145 150 155 160
Glu Leu Leu Thr Pro His Gln Glu Met Met Met Thr Met Met Asp Gln
165 170 175
Gln Gln Gln Gln Leu Leu Tyr Gln Glu Ala Val Asp Ser Leu Val Asn
180 185 190
Ser Pro Asn Ser Asn Lys Leu Ile Met Ser His Gln Glu Asp Ser Arg
195 200 205
Glu Gln Ser Thr Asn Lys Gly Ile Met Leu Leu Ser Asp Val Arg Ser
210 215 220
Gly Ser Ser Thr Thr Ser Thr Val Thr Arg Val Lys Met Glu Gln His
225 230 235 240
Asp His His His Glu Glu Arg Ser Met Glu Asp Tyr Gly Met Glu Glu
245 250 255
Ile Asn His Leu Ile Asn Ser Ser Cys Thr Ser Ser Ser Ser Asn Ser
260 265 270
Leu Trp Phe Asp Glu Asn Lys Thr Asp Asp Thr Phe Met Leu Tyr Tyr
275 280 285
<210>63
<211>792
<212>DNA
<213>芜菁
<220>
<221>其他特征
<222>(760)..(760)
<223>n可以是任意核苷
<400>63
atgggaagag caccgtgttg tgacaaggct aacgtgaaga aagggccttg gtctcctgaa 60
gaagatgcaa agctcaaaga ttacatcgag agtagtggca caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcagactaag gtggcttaac 180
tatttgagac ctaacatcaa acatggtggc ttctctgagg aagaggacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaatt gcctggaaga 300
acagacaacg atatcaagaa ctattggaac acgaggctga agaagaagct tcttaacaaa 360
caaagaaaag agttccaaga agctcggatg aagcaagaga tggtgatgat gaaacgacag 420
caacaaggac aaggacaatg ccaaagtaat ggtagtacgg atctttatct gaacaacatg 480
tttagatcat caccatggcc attactgcct catcttcctc ctcctcatag tcaagtacct 540
cttgtgatga tggaaccaac aagctgcaat tactaccaac cgacaccgtc ttgcgcatta 600
gaacaaaagc cattgatccc actcaagaac atggtcaaga ttgaagcaga accggagaga 660
tcaaaccctg atcatcatta tccggaagac tcaatgacaa actcttttga tctttccttc 720
tctcagcttt tgttagatcc taattactac ctggaatcan gaggaggaga aaggagagtt 780
tgctctcatg ag 792
<210>64
<211>264
<212>PRT
<213>芜菁
<220>
<221>其他特征
<222>(254)..(254)
<223>X可以是任意氨基酸
<400>64
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Ser Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Gly Gln
130 135 140
Gly Gln Cys Gln Ser Asn Gly Ser Thr Asp Leu Tyr Leu Asn Asn Met
145 150 155 160
Phe Arg Ser Ser Pro Trp Pro Leu Leu Pro His Leu Pro Pro Pro His
165 170 175
Ser Gln Val Pro Leu Val Met Met Glu Pro Thr Ser Cys Asn Tyr Tyr
180 185 190
Gln Pro Thr Pro Ser Cys Ala Leu Glu Gln Lys Pro Leu Ile Pro Leu
195 200 205
Lys Asn Met Val Lys Ile Glu Ala Glu Pro Glu Arg Ser Asn Pro Asp
210 215 220
His His Tyr Pro Glu Asp Ser Met Thr Asn Ser Phe Asp Leu Ser Phe
225 230 235 240
Ser Gln Leu Leu Leu Asp Pro Asn Tyr Tyr Leu Glu Ser Xaa Gly Gly
245 250 255
Glu Arg Arg Val Cys Ser His Glu
260
<210>65
<211>558
<212>DNA
<213>芜菁
<400>65
atgggaaggg caccgtgttg tgacaaagcc aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcca aactcaaaga ttacatcgag aatagtggta caggaggcaa ctggatcgct 120
ttgcctcaaa agattggtct aaggagatgt gggaagagtt gcagactaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aagaagacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaact gtcgggaaga 300
acggacaacg atatcaagaa ctattggaac acgaggctaa agaagaagct tctaaacaaa 360
caaaggaaag agtttcaaga agctcggatg aagcaagaga tggtgatgat gaagagacaa 420
caagaaggac atgaccacat caatggtagt acggatcttt atctgaaaaa tatgtttgga 480
tcatcaccat ggccattact acaacagctt cctctgttgg gattgcgaaa tcccgtgtcc 540
aactctatct tatcttat 558
<210>66
<211>186
<212>PRT
<213>芜菁
<400>66
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Ser Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Glu Gly His
130 135 140
Asp His Ile Asn Gly Ser Thr Asp Leu Tyr Leu Lys Asn Met Phe Gly
145 150 155 160
Ser Ser Pro Trp Pro Leu Leu Gln Gln Leu Pro Leu Leu Gly Leu Arg
165 170 175
Asn Pro Val Ser Asn Ser Ile Leu Ser Tyr
180 185
<210>67
<211>501
<212>DNA
<213>红花
<400>67
atgataaggg caccctgctg cgacaaaacc aatgtgaaaa agggaccatg gtcttctgaa 60
gaagatgcta agctcaaaga ttacatcgga aagtatggta ccggcggtaa ctggatcgct 120
cttccacaaa aaatcgagct caagatctgt gggacaaggt gcagattgag atggttgaat 180
tacctgagac ccaacatcaa gcatggggga ttctctgaag aagaagacaa catcatctgc 240
agcctctata ttagcatagg cagcaggtgg tccataattg cggctcaatt gccaggcaga 300
accgataacg acgtcaagaa ctattgcaac accgagctga agaagatatt actcggaggg 360
cgcaaacaat cccaggccaa taataagctc tcgggtgatc cgaaagacag caggcggata 420
gaaacgctaa gcaacttcgg gatcaaaagg ctccaactgc acaggcgact cggaagcctt 480
gaagacccta atttctgtca c 501
<210>68
<211>167
<212>PRT
<213>红花
<400>68
Met Ile Arg Ala Pro Cys Cys Asp Lys Thr Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Ser Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Gly Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Glu Leu Lys
35 40 45
Ile Cys Gly Thr Arg Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Cys Asn Thr Glu
100 105 110
Leu Lys Lys Ile Leu Leu Gly Gly Arg Lys Gln Ser Gln Ala Asn Asn
115 120 125
Lys Leu Ser Gly Asp Pro Lys Asp Ser Arg Arg Ile Glu Thr Leu Ser
130 135 140
Asn Phe Gly Ile Lys Arg Leu Gln Leu His Arg Arg Leu Gly Ser Leu
145 150 155 160
Glu Asp Pro Asn Phe Cys His
165
<210>69
<211>749
<212>DNA
<213>红花
<400>69
atggggagag ccccttgttg tgataaagca aatgtgaaga aaggtccatg gtctcaagaa 60
gaagatacaa aactcaaaga gtttattgaa aaatttggta ctggtggtaa ctggattgtt 120
ctccctcaaa aagctggtct gaaaagatgt gggaagagtt gcaggttgag atggcttaac 180
tatctaagac ccaatatcag gcatggtgaa ttctctgatg atgaagacag gatcatctgc 240
agcttgtatg ctacatttgg tagcagatgg tcagtaatag cagctcagct accaggaagg 300
actgacaatg atatcaagaa ctattggaac accaaactca agaagaagct cttcactatg 360
cttccttccc ttcaagaaaa acaaactttc tttccatctt tgcccttcca accaccacca 420
ccatatgatc accaccacca tcatcatcac ccatcagacc cattcttcac cacttcatca 480
tcatcatcat catcattcta caccagttac aaccctaact ttatgaatct taacaccact 540
tccaattctc tcattgttca agatcatgat gttaatgttg ttcaacacaa tctctcacca 600
ccaccgccac caccaccacc tgtgaaccat ttgatcaatt tgctgagcaa caacaccatt 660
gatgttaatg tgattgatga tcataactcc tacagttatc atctagggtt tcaagatgac 720
caccatgatc agagcatgtg caacagttc 749
<210>70
<211>250
<212>PRT
<213>红花
<400>70
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Gln Glu Glu Asp Thr Lys Leu Lys Glu Phe Ile Glu Lys Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Val Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Glu Phe Ser Asp Asp Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Thr Phe Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Phe Thr Met Leu Pro Ser Leu Gln Glu Lys Gln
115 120 125
Thr Phe Phe Pro Ser Leu Pro Phe Gln Pro Pro Pro Pro Tyr Asp His
130 135 140
His His His His His His Pro Ser Asp Pro Phe Phe Thr Thr Ser Ser
145 150 155 160
Ser Ser Ser Ser Ser Phe Tyr Thr Ser Tyr Asn Pro Asn Phe Met Asn
165 170 175
Leu Asn Thr Thr Ser Asn Ser Leu Ile Val Gln Asp His Asp Val Asn
180 185 190
Val Val Gln His Asn Leu Ser Pro Pro Pro Pro Pro Pro Pro Pro Val
195 200 205
Asn His Leu Ile Asn Leu Leu Ser Asn Asn Thr Ile Asp Val Asn Val
210 215 220
Ile Asp Asp His Asn Ser Tyr Ser Tyr His Leu Gly Phe Gln Asp Asp
225 230 235 240
His His Asp Gln Ser Met Cys Asn Ser Ser
245 250
<210>71
<211>648
<212>DNA
<213>斑点矢车菊
<400>71
atgggaaggg caccttgttg tgacaaagca aacgttaaga aagggccatg gtctcccgaa 60
gaagatgcta aactcaaaga ttatatcgaa aaatatggta ccgggggtaa ctggatcgct 120
cttccgcaga agattggcat aaagagatgt ggaaaaagtt gtagactaag gtggttgaat 180
tacctaagac ctaatatcaa gcatggagga ttctctgaag aagaagacaa catcatctgc 240
agcctctata ttagtatcgg cagcaggtgg tccataattg ctgcccaact gcctggaaga 300
actgataatg atatcaagaa ctattggaac actaggctaa agaagaaatt gcttggaagg 360
cgaaaacagt ctcatgctag taggctcggc accggttcaa accaggatcc caaagatggg 420
aacgggctag aaacgttaag caattcggcc atcgaaaggc ttcaacttca catgcaactt 480
caaagccttg aaaaccctaa tatcgctaat catggtgtta atctcgcgac gtggccttgt 540
aagctgaacc cgattcaaga gaagatgatg cagagtcttc aacttctaaa tgagtcgcca 600
aaccctctaa tgatgcaacc tcattcccct caaaaggttg aactttat 648
<210>72
<211>216
<212>PRT
<213>斑点矢车菊
<400>72
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Ile Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Ser His Ala Ser Arg
115 120 125
Leu Gly Thr Gly Ser Asn Gln Asp Pro Lys Asp Gly Asn Gly Leu Glu
130 135 140
Thr Leu Ser Asn Ser Ala Ile Glu Arg Leu Gln Leu His Met Gln Leu
145 150 155 160
Gln Ser Leu Glu Asn Pro Asn Ile Ala Asn His Gly Val Asn Leu Ala
165 170 175
Thr Trp Pro Cys Lys Leu Asn Pro Ile Gln Glu Lys Met Met Gln Ser
180 185 190
Leu Gln Leu Leu Asn Glu Ser Pro Asn Pro Leu Met Met Gln Pro His
195 200 205
Ser Pro Gln Lys Val Glu Leu Tyr
210 215
<210>73
<211>693
<212>DNA
<213>斑点矢车菊
<400>73
atgggaaggg caccttgttg tgacaaagca aacgttaaga aagggccatg gtctcccgaa 60
gaagatgcta aactcaaaga ttatatcgaa aaatatggta ccgggggtaa ctggatcgct 120
cttccgcaga agattggcat aaagagatgt ggaaaaagtt gtagactaag gtggttgaat 180
tacctaagac ctaatatcaa gcatggagga ttctctgaag aagaagacaa catcatctgc 240
agcctctata ttagtatcgg cagcaggtgg tccataattg ctgcccaact gcctggaaga 300
actgataatg atatcaagaa ctattggaac actaggctaa agaggaaatt gcttggaagg 360
cgaaaacagt ctcatgctag taggctcggc accggttcaa accaggatcc caaagatggg 420
aacgggctag aaacgttaag caattcggcc atcgaaaggc ttcaacttca catgcaactt 480
caaagccttg aaaaccctaa tatcgctaat catggtgtta atctcgcgac gtggccttgt 540
aagctgaacc cgattcaaga gaagatgatg cagagtcttc aacttctaaa tgagtcgcca 600
aaccctctaa tgatgcaacc tcattcccct caaaaggttg aactttatgc acaatcaaat 660
aactttcttc aacaagtgta ctctccatcg agc 693
<210>74
<211>231
<212>PRT
<213>斑点矢车菊
<400>74
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Ile Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Arg Lys Leu Leu Gly Arg Arg Lys Gln Ser His Ala Ser Arg
115 120 125
Leu Gly Thr Gly Ser Asn Gln Asp Pro Lys Asp Gly Asn Gly Leu Glu
130 135 140
Thr Leu Ser Asn Ser Ala Ile Glu Arg Leu Gln Leu His Met Gln Leu
145 150 155 160
Gln Ser Leu Glu Asn Pro Asn Ile Ala Asn His Gly Val Asn Leu Ala
165 170 175
Thr Trp Pro Cys Lys Leu Asn Pro Ile Gln Glu Lys Met Met Gln Ser
180 185 190
Leu Gln Leu Leu Asn Glu Ser Pro Asn Pro Leu Met Met Gln Pro His
195 200 205
Ser Pro Gln Lys Val Glu Leu Tyr Ala Gln Ser Asn Asn Phe Leu Gln
210 215 220
Gln Val Tyr Ser Pro Ser Ser
225 230
<210>75
<211>651
<212>DNA
<213>斑点矢车菊
<400>75
atgggaagag ctccatgttg tgataaggca aatgtgaaga aagggccatg gtcacctgaa 60
gaagatagaa agctaaaaga gtacatagaa actcatggta ctggtggcaa ctggattgct 120
ctcccacaaa aagcaggcct tagaagatgt ggaaaaagct gcagattaag atggttgaac 180
tatctaagac caaacatcaa acatggtgaa ttttctgatg atgaagataa agttatctgt 240
gcactctttg ctagcattgg cagcaggtgg tcaataatgg cagcacagtt accaggaagg 300
acagacaatg atataaagaa ctactggaac acaaagctga agaagaagat gatgagcagc 360
ttaatctcca ttcctgaaat cagaaaacca tttcatcatc aacatcttga atctttcaaa 420
tcttcatcca actacatgag ctacccatca tcactctcta tttttcaaaa cagtaataat 480
atcagtgctc cattatcttc atcatcacca ccttcatcat atctctataa tagtacaaca 540
acaactactt ctactcatca tcatcatgat catcatcatc aaaccctatc tatcccatca 600
agccctactc ctgctcatgg atataatctg ggtttgggtc ccatggtggt a 651
<210>76
<211>217
<212>PRT
<213>斑点矢车菊
<400>76
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Arg Lys Leu Lys Glu Tyr Ile Glu Thr His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Lys Val Ile Cys
65 70 75 80
Ala Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Ile Met Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Met Met Ser Ser Leu Ile Ser Ile Pro Glu Ile Arg
115 120 125
Lys Pro Phe His His Gln His Leu Glu Ser Phe Lys Ser Ser Ser Asn
130 135 140
Tyr Met Ser Tyr Pro Ser Ser Leu Ser Ile Phe Gln Asn Ser Asn Asn
145 150 155 160
Ile Ser Ala Pro Leu Ser Ser Ser Ser Pro Pro Ser Ser Tyr Leu Tyr
165 170 175
Asn Ser Thr Thr Thr Thr Thr Ser Thr His His His His Asp His His
180 185 190
His Gln Thr Leu Ser Ile Pro Ser Ser Pro Thr Pro Ala His Gly Tyr
195 200 205
Asn Leu Gly Leu Gly Pro Met Val Val
210 215
<210>77
<211>732
<212>DNA
<213>黄星蓟
<400>77
atggggagag caccttgctg tgataaagcc aatgtgaaga aaggtccatg ggctcctgaa 60
gaagatgcta ctcttaaagc ttatattgaa gaacatggta ctggtggtaa ctggattgct 120
ttgcctcaga aaatagggct taaaagatgc gggaagagtt gtcgcctgcg gtggctaaat 180
tatctccgtc cgaatatcaa gcatggaggt ttttcggaag aagaagatcg cataatttgc 240
agcctctatg ttagcatagg gagcaggtgg tctataatcg cagcacaatt acctggacga 300
actgataacg atataaagaa ctactggaat acaaggctga agaagaaact cttgggtaag 360
caacgaaaag aacaaatttc tcgtagaaag ggggagatgc taatgaagaa agggagatcg 420
ccatcggaaa ttccaccttc cgtaatcgtt agtggtaacg ataccaataa caattgcccg 480
gatccttatt ggccagagtt gccggttttg ccacctgcgc cctattcgat ccaagaacca 540
tgctttgcca acggtcatgc ctctatacga aaactactca tgaagcttgg aggaaggttt 600
tcttgtgatg acaatggaaa ccagtccatc aacatggtct cacactttcc cattgatcat 660
cttccaacat cattaatgca tgatgatcac aactttattg ctagtagtac tactccggct 720
tcttcttcag ct 732
<210>78
<211>244
<212>PRT
<213>黄星蓟
<400>78
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ala Pro Glu Glu Asp Ala Thr Leu Lys Ala Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Ile Ser Arg
115 120 125
Arg Lys Gly Glu Met Leu Met Lys Lys Gly Arg Ser Pro Ser Glu Ile
130 135 140
Pro Pro Ser Val Ile Val Ser Gly Asn Asp Thr Asn Asn Asn Cys Pro
145 150 155 160
Asp Pro Tyr Trp Pro Glu Leu Pro Val Leu Pro Pro Ala Pro Tyr Ser
165 170 175
Ile Gln Glu Pro Cys Phe Ala Asn Gly His Ala Ser Ile Arg Lys Leu
180 185 190
Leu Met Lys Leu Gly Gly Arg Phe Ser Cys Asp Asp Asn Gly Asn Gln
195 200 205
Ser Ile Asn Met Val Ser His Phe Pro Ile Asp His Leu Pro Thr Ser
210 215 220
Leu Met His Asp Asp His Asn Phe Ile Ala Ser Ser Thr Thr Pro Ala
225 230 235 240
Ser Ser Ser Ala
<210>79
<211>675
<212>DNA
<213>黄星蓟
<400>79
atggggagag caccttgctg tgataaagcc aatgtgaaga aaggtccatg ggctcctgaa 60
gaagatgcta ctcttaaagc ttatattgaa gaacatggta ctggtggtaa ctggattgct 120
ttgcctcaga aaatagggct taaaagatgc gggaagagtt gtcgcctgcg gtggctaaat 180
tatctccgtc cgaatatcaa gcatggaggt ttttcggaag aagaagatcg cataatttgc 240
agcctctatg ttagcatagg gagcaggtgg tctataatcg caccacaatt acctggacga 300
actgataacg atataaagaa ctactggaat acaaggctga agaagaaact cttgggtaag 360
caacgaaaag aacaaatttc tcgtaaaaag ggggagatgc taatgaagaa agggagatcg 420
ccatcggaaa ttccatcttc cgtaatcgtt agtggtaacg ataccaataa caattgcccg 480
gatccttatt ggccagagtt gccggttttg ccacctgcgc cctattcgat ccaagaacca 540
tgctttgcca acggtcatgc ctctatacga aaactactca tgaagcttgc aggaaggttt 600
tcttgtgatg acaatggaaa ccagtccatc aacatggtct cacacttacc cattgatcat 660
cttacgacat catta 675
<210>80
<211>225
<212>PRT
<213>黄星蓟
<400>80
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ala Pro Glu Glu Asp Ala Thr Leu Lys Ala Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Pro Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Ile Ser Arg
115 120 125
Lys Lys Gly Glu Met Leu Met Lys Lys Gly Arg Ser Pro Ser Glu Ile
130 135 140
Pro Ser Ser Val Ile Val Ser Gly Asn Asp Thr Asn Asn Asn Cys Pro
145 150 155 160
Asp Pro Tyr Trp Pro Glu Leu Pro Val Leu Pro Pro Ala Pro Tyr Ser
165 170 175
Ile Gln Glu Pro Cys Phe Ala Asn Gly His Ala Ser Ile Arg Lys Leu
180 185 190
Leu Met Lys Leu Ala Gly Arg Phe Ser Cys Asp Asp Asn Gly Asn Gln
195 200 205
Ser Ile Asn Met Val Ser His Leu Pro Ile Asp His Leu Thr Thr Ser
210 215 220
Leu
225
<210>81
<211>783
<212>DNA
<213>黄星蓟
<400>81
atgggaagag caccttgctg tgacaaagcc aacgtcaaaa gaggaccatg gtcacctgaa 60
gaagatgcca aactcaaatc gtatatcgaa gaacatggaa ccggaggcaa ctggatcgct 120
ctacctcata agattggtct taagaggtgt ggcaagagtt gtcgccttcg atggttgaat 180
tatctacgcc caaatatcaa gcatggatct ttctccgaag aagaagatca catcatttgc 240
accctttatc tcagtattgg cagccgatgg tcaattatag ctgcacaact acctgggcga 300
accgataatg acatcaagaa ctactggaac acgaggctaa agaaaaagct tttgggaaaa 360
cagcgtaaag atcaacaatc ttcgaagaaa agtggtttga tcaagcaaga aatgaagaga 420
gaagttcttg aagatttcaa ggcaccatct ttatgcatga gcatgaacct ctatcaatct 480
tactggccta cagatttccc taatttgatc cttacaaacc ctgatcatga tcatcatcaa 540
gagcaaccac ttcatgccaa aaaccaagat cccatcacaa gttttgatga tcaataccct 600
tttgatacct ccccaaatca agcccaattg ttcgatcaaa accacgagaa acctatttca 660
accattaatt atcatcccca attgcaaaac acaccatata atcttgtgat caatggagat 720
caaggtgtga atctttttca agaattcaac aactacccat ttgggatcaa tgaattggtc 780
tac 783
<210>82
<211>261
<212>PRT
<213>黄星蓟
<400>82
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Cys
65 70 75 80
Thr Leu Tyr Leu Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Asp Gln Gln Ser Ser
115 120 125
Lys Lys Ser Gly Leu Ile Lys Gln Glu Met Lys Arg Glu Val Leu Glu
130 135 140
Asp Phe Lys Ala Pro Ser Leu Cys Met Ser Met Asn Leu Tyr Gln Ser
145 150 155 160
Tyr Trp Pro Thr Asp Phe Pro Asn Leu Ile Leu Thr Asn Pro Asp His
165 170 175
Asp His His Gln Glu Gln Pro Leu His Ala Lys Asn Gln Asp Pro Ile
180 185 190
Thr Ser Phe Asp Asp Gln Tyr Pro Phe Asp Thr Ser Pro Asn Gln Ala
195 200 205
Gln Leu Phe Asp Gln Asn His Glu Lys Pro Ile Ser Thr Ile Asn Tyr
210 215 220
His Pro Gln Leu Gln Asn Thr Pro Tyr Asn Leu Val Ile Asn Gly Asp
225 230 235 240
Gln Gly Val Asn Leu Phe Gln Glu Phe Asn Asn Tyr Pro Phe Gly Ile
245 250 255
Asn Glu Leu Val Tyr
260
<210>83
<211>768
<212>DNA
<213>菊苣
<400>83
atgggaagag caccttgctg tgacaaagcc aacgtcaaaa gaggaccttg gtcacctgaa 60
gaagatgcca aactcaagtc ttacattgaa gaacatggaa cgggaggcaa ctggatcgct 120
ctacctcata aaatcggact caagaggtgt ggaaagagtt gtcgccttcg atggttaaat 180
tatcttcgcc caaacatcaa gcacggatct ttttccgaag aagaagatca cataatttgt 240
accctatatc ttagtattgg cagccggtgg tcaattatcg ctgcacaatt acccgggaga 300
actgataatg atatcaagaa ctactggaac acaaggctaa agaaaaagct tttgggaaag 360
cagcgtaaag atcaacaatt gtcaaagaga agtgcccaaa taaagcatga aatgaaggag 420
agagtggttc ttgaggattt aaaggcacca tctttatgca tgagcatgaa tctttatcaa 480
tcatactggc ctgctgaatt cccttcgact ctcacggacc atgatcagca tcatcaagaa 540
catcatgtca aaacccaaga acccgttttc aatgaatacc cttttgacat aatttcaaac 600
caagcacaat tgttctacca gaacagcgag aaacctatct cgactactgt ttaccatccc 660
cagttggcaa acatacccta ttttggaatt ggggatggca taaacttgtt tcaagaattc 720
aacaattacc catttgaaat caatgaattg gtttacaaca ccacacaa 768
<210>84
<211>256
<212>PRT
<213>菊苣
<400>84
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Cys
65 70 75 80
Thr Leu Tyr Leu Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Asp Gln Gln Leu Ser
115 120 125
Lys Arg Ser Ala Gln Ile Lys His Glu Met Lys Glu Arg Val Val Leu
130 135 140
Glu Asp Leu Lys Ala Pro Ser Leu Cys Met Ser Met Asn Leu Tyr Gln
145 150 155 160
Ser Tyr Trp Pro Ala Glu Phe Pro Ser Thr Leu Thr Asp His Asp Gln
165 170 175
His His Gln Glu His His Val Lys Thr Gln Glu Pro Val Phe Asn Glu
180 185 190
Tyr Pro Phe Asp Ile Ile Ser Asn Gln Ala Gln Leu Phe Tyr Gln Asn
195 200 205
Ser Glu Lys Pro Ile Ser Thr Thr Val Tyr His Pro Gln Leu Ala Asn
210 215 220
Ile Pro Tyr Phe Gly Ile Gly Asp Gly Ile Asn Leu Phe Gln Glu Phe
225 230 235 240
Asn Asn Tyr Pro Phe Glu Ile Asn Glu Leu Val Tyr Asn Thr Thr Gln
245 250 255
<210>85
<211>639
<212>DNA
<213>菊苣
<400>85
atgggaaggg ctccatgttg tgacaaagaa aatgtaaaga gagggccatg gtctcctgaa 60
gaagatgcaa aactcaaaag cttcattgac aaaaacggca ctggtggtaa ctggattgct 120
ctccctcaca aagctggtct aaaaagatgt ggcaagagct gcagattgag atggctaaac 180
tatctgaggc ccaatattaa gcatggtgaa ttcactgatg atgaagacaa gatcatctgc 240
agcttgtatg ctagcattgg tagcagatgg tcaataatag cagctcagct accaggaagg 300
actgataatg atatcaagaa ttactggaac accaagctca agaagaagct cttggctatg 360
cttccttcct ttcaaaagaa gtcatcttta tttccatcta catccctcca atcaccatca 420
ccatacagat caaatcagtt catgaccaat aattcgtcac ctttttacgg ttataccact 480
acccctaact tcatgaacat gaacaacaat atgatcagtt ctctgccgag acctaccagc 540
accaccacct gtgttcaaga tcatatcact catctctcac caccaccacc gctagatcca 600
aactctttga tcagtttaat gaccagcaac gttgacaac 639
<210>86
<211>213
<212>PRT
<213>菊苣
<400>86
Met Gly Arg Ala Pro Cys Cys Asp Lys Glu Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Phe Ile Asp Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Thr Asp Asp Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Ala Met Leu Pro Ser Phe Gln Lys Lys Ser
115 120 125
Ser Leu Phe Pro Ser Thr Ser Leu Gln Ser Pro Ser Pro Tyr Arg Ser
130 135 140
Asn Gln Phe Met Thr Asn Asn Ser Ser Pro Phe Tyr Gly Tyr Thr Thr
145 150 155 160
Thr Pro Asn Phe Met Asn Met Asn Asn Asn Met Ile Ser Ser Leu Pro
165 170 175
Arg Pro Thr Ser Thr Thr Thr Cys Val Gln Asp His Ile Thr His Leu
180 185 190
Ser Pro Pro Pro Pro Leu Asp Pro Asn Ser Leu Ile Ser Leu Met Thr
195 200 205
Ser Asn Val Asp Asn
210
<210>87
<211>832
<212>DNA
<213>菊苣
<400>87
tgggaagagc tccatgttgt gacaagtcaa aggtgaagaa agggccatgg tcgcctgagg 60
aagatacgaa gttgaaagac tatatacaca aaaatggtac tggaggcaac tggattgctc 120
ttccacataa agcaggcctt aaaagatgtg gaaaaagctg cagattgcga tggttgaact 180
accttagacc agacatcaaa cacggtgaat tctccgatca tgaagataga ctcatctgta 240
ctctcttttc tagcatcggt agcaggtggt cagttatagc agcacagttg cctggaagaa 300
cggataatga tatcaagaac tactggaaca cgaagctcaa aaagaagctt atgaatttca 360
tctccaccaa tcgtcaaatt gggaaaccgc ttcgtcatct tgatcaatct atcaattgta 420
cttcttcaaa ttggagctac ccaacaagct cttctgctat aactgttgat aatcatccag 480
ttctaccatc agatgatcat ggatacatta atgtagaccc actggagacc tatcaagtaa 540
aagacgattc tacccttatg tttgaaggcg gtgatcaagc tgctggttgt acttctactt 600
cggattggcg gtaccatcat gtgtatggtg gtggtgtgtt tgatgctcat aataacatgg 660
gagtcttgaa gaccggtgat tcttacaaga gagatgatgg gaattgtgaa aaggcaagtg 720
ggtattatgg ggattctaca ttggagtttg gtcttgagga gttcaagaag ctcattagca 780
ctaatctgtg cagcggtaac actaacaata atctcaatgt ctttgttgat ga 832
<210>88
<211>435
<212>DNA
<213>脐橙
<400>88
atgggaagag ctccatgttg tgacaaagct aacgttaaaa gaggaccttg gtctgctgaa 60
gaagactcga ttctcaaaaa ttaccttgag caatttggaa atggcggcaa ctggattgct 120
ttacccaaga aagcaggcct taatagatgt ggcaagagtt gtcggctcag atggctaaat 180
tatctaaggc cagatattaa gcatggagga tttactaagg aagaagatac catcatatgc 240
aatctttatt gtaccatggg aagtaggtgg tctgttatag cttctcagct gccaggaaga 300
actgacaatg atgtaaagaa ttattggaac accaagttga agaaaaatgt tttggcagga 360
aagctgtcgg acaacactca agtttcagtt tcaacaattc ctgaagaatt tggaaattca 420
tcttactact tgagt 435
<210>89
<211>145
<212>PRT
<213>脐橙
<400>89
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Ala Glu Glu Asp Ser Ile Leu Lys Asn Tyr Leu Glu Gln Phe
20 25 30
Gly Asn Gly Gly Asn Trp Ile Ala Leu Pro Lys Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Lys Glu Glu Asp Thr Ile Ile Cys
65 70 75 80
Asn Leu Tyr Cys Thr Met Gly Ser Arg Trp Ser Val Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Asn Val Leu Ala Gly Lys Leu Ser Asp Asn Thr Gln Val
115 120 125
Ser Val Ser Thr Ile Pro Glu Glu Phe Gly Asn Ser Ser Tyr Tyr Leu
130 135 140
Ser
145
<210>90
<211>685
<212>DNA
<213>中果咖啡
<400>90
gcacgcaggg aaattaatcc acacacacac acacactcaa gcatttcaaa agcactcgaa 60
agaagcctgg atagagagaa gcagaaacag gcacagagtg taacaaccag agagagatgg 120
gaagagctcc ttgctgtgac aaggcgaatg tgaagaaagg accttggtcc cctgaagaag 180
atgcaaagct gaaggagtac atagaaaagt cagggactgg agggaattgg atagctcttc 240
cacacaaggc tggtaagccc acttatgaat ttccgtgtaa ttacaacact agtatttgtt 300
atggtttgcc agcagttggt tggcataatt atgatcaggg cttagaagat gcggaaagag 360
ttgcagattg agatggctta actacctcag gcccaacatt aaacatggag acttcacaga 420
tgatgaggat aggataattt gcagcctctt tgcgagcatt ggtagcaggt ggtcaataat 480
agcagctcaa ttaccaggca ggacggacaa tgacatcaag aactactgga acaccaagct 540
gaaaaagaaa ataatgggat ctcacaaaaa atgccatcaa cccagcccct acgcatcacc 600
atattcgggg tttgagcctg catctttaac atcatcacca ttttcagctc catcaccatc 660
aatatcatcg gcgtatcaca attat 685
<210>91
<211>731
<212>DNA
<213>中果咖啡
<400>91
acacacactc aagcatttca aaagcactcg aaccctgcct ggatagagag aagcagaaac 60
aggcacagag tgtaacaacc agagagagat gggaagagct ccttgctgtg acaaggcgaa 120
tgtgaagaaa ggaccttggt cccctgaaga agatgcaaag ctgaaggagt acatagaaaa 180
gtcagggact ggagggaatt ggatagctct tccacacaag gctggtaagc ccacttatga 240
atttccgtgt aattacaaca ctagtatttg ttatggtttg ccagcagttg gttggcataa 300
ttatgatcag ggcttagaag atgcggaaag agttgcagat tgagatggct taactacctc 360
aggcccaaca ttaaacatgg agacttcaca gatgatgagg ataggataat ttgcagcctc 420
tttgcgagca ttggtagcag gtggtcaata atagcagctc aattaccagg caggacggac 480
aatgacatca agaactactg gaacaccaag ctgaaaaaga aaataatggg atctcacaaa 540
aaatgccatc aacccagccc ctacgcatca ccatattcgg ggtttgagcc tgcatcttta 600
acatcatcac cattttcagc tccatcacca tcaatatcat cggcgtatca caattatcac 660
accaccaccc aatttaggtc cctctcacgc tatgaaactt tttcatcaac cccgccaagt 720
ctgttgaacg c 731
<210>92
<211>711
<212>DNA
<213>甜瓜
<400>92
atggggagag ctccttgctg tgacaaagca aacgtgaaga agggtccgtg gtcgccggag 60
gaagacatga aactcaaatc ctatatcgag cagcacggta caggtgggaa ctggattgca 120
ctgccccaga aaattggtct gaagagatgt gggaaaagct gccgactccg gtggttgaac 180
taccttcggc ctaatattaa gcatggggat ttttctgaag aagaagataa aataatttgc 240
agcctttatg tcagcattgg aagcaggtgg tcgattattg cagctcaatt accgggacga 300
acagacaacg atataaagaa ttattggaac acaagattga agaagaaatt atttggaaaa 360
cattatgaga aacaacaaca acaattggtg agaagaggaa gaaaaattaa atccggaata 420
ggaaattcca tggctattgt ttttgataat caaggtattt acaataataa taataacaac 480
caatcgcctt tcttgccgaa attatcaccg gcgctcaatt caccgccacc accgccgtac 540
gctaattatc agccgcttca gttccattca aattcgttca gcaacaccgc cggcgttatt 600
gatgatctga ggagaaccga cagtgtactc cagtttggaa tggatggggc ttcgtgtcag 660
ccagtaacga gcacccacgt cggtgagttg ggagaaaggt tgtttacagc a 711
<210>93
<211>237
<212>PRT
<213>甜瓜
<400>93
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Met Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys His Tyr Glu Lys Gln Gln Gln Gln
115 120 125
Leu Val Arg Arg Gly Arg Lys Ile Lys Ser Gly Ile Gly Asn Ser Met
130 135 140
Ala Ile Val Phe Asp Asn Gln Gly Ile Tyr Asn Asn Asn Asn Asn Asn
145 150 155 160
Gln Ser Pro Phe Leu Pro Lys Leu Ser Pro Ala Leu Asn Ser Pro Pro
165 170 175
Pro Pro Pro Tyr Ala Asn Tyr Gln Pro Leu Gln Phe His Ser Asn Ser
180 185 190
Phe Ser Asn Thr Ala Gly Val Ile Asp Asp Leu Arg Arg Thr Asp Ser
195 200 205
Val Leu Gln Phe Gly Met Asp Gly Ala Ser Cys Gln Pro Val Thr Ser
210 215 220
Thr His Val Gly Glu Leu Gly Glu Arg Leu Phe Thr Ala
225 230 235
<210>94
<211>831
<212>DNA
<213>甜瓜
<400>94
atggggagag ctccttgctg tgacaaagca aacgtgaaga agggtccgtg gtcgccggag 60
gaagacatga aactcaaatc ctacatcgag cagcacggta caggtgggaa ctggattgca 120
ctgccccaga aaattggtct gaagagatgt gggaaaagct gccgactccg gtggttgaac 180
taccttcggc ctaatattaa gcatggggat ttttctgaag aagaagataa aataatttgc 240
agcctttatg tcagcattgg aagcaggtgg tcgattattg cagctcaatt accgggacga 300
acagacaacg atataaagaa ttattggaac acaagattga agaagaaatt atttggaaaa 360
cattatgaga aacaacaaca acaattggtg agaagaggaa gaaaaattaa atccggaata 420
ggaaattcca tggctattgt ttttgataat caaggtattt acaataataa taataacaac 480
caatcgcctt tcttgccgaa attatcaccg gcgctcaatt caccgccacc accgccgtac 540
gctaattatc agccgcttca gttccattca aattcgttca gcaacaccgc cggcgttatt 600
gatgatctga ggagaaccga cagtgtactc cagtttggaa tggatggggc ttcgtgtcag 660
ccagtaacga gcacccacgt cggtgagttg gagaaggttg tttacagcaa tactccgtcg 720
tttgacgatg ggctgcagtt ttcatgtgat aacaatgggt tgaatttgat gaacgatttg 780
gattgggggg aaatgagttc tttgatttct gctcctttat atccttcgat g 831
<210>95
<211>277
<212>PRT
<213>甜瓜
<400>95
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Met Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys His Tyr Glu Lys Gln Gln Gln Gln
115 120 125
Leu Val Arg Arg Gly Arg Lys Ile Lys Ser Gly Ile Gly Asn Ser Met
130 135 140
Ala Ile Val Phe Asp Asn Gln Gly Ile Tyr Asn Asn Asn Asn Asn Asn
145 150 155 160
Gln Ser Pro Phe Leu Pro Lys Leu Ser Pro Ala Leu Asn Ser Pro Pro
165 170 175
Pro Pro Pro Tyr Ala Asn Tyr Gln Pro Leu Gln Phe His Ser Asn Ser
180 185 190
Phe Ser Asn Thr Ala Gly Val Ile Asp Asp Leu Arg Arg Thr Asp Ser
195 200 205
Val Leu Gln Phe Gly Met Asp Gly Ala Ser Cys Gln Pro Val Thr Ser
210 215 220
Thr His Val Gly Glu Leu Glu Lys Val Val Tyr Ser Asn Thr Pro Ser
225 230 235 240
Phe Asp Asp Gly Leu Gln Phe Ser Cys Asp Asn Asn Gly Leu Asn Leu
245 250 255
Met Asn Asp Leu Asp Trp Gly Glu Met Ser Ser Leu Ile Ser Ala Pro
260 265 270
Leu Tyr Pro Ser Met
275
<210>96
<211>939
<212>DNA
<213>胡萝卜
<400>96
atggggagag ctccttgctg tgacaaggca agtgtaaaga gagggccatg gtcacctgaa 60
gaagatcaga agctaaaaga ctatatagag aagcatggga ctggaggaaa ctggattgct 120
ctaccacaaa aggctggtct tagaagatgt gggaaaagct gcagattaag atggctcaac 180
tatctcaggc ctaacattaa acatggagaa ttttcagatg atgaagatag gatcatatgc 240
accctctttg ccaatattgg gagcaggtgg tcaataatag caggtcagtt accagggagg 300
actgacaatg atatcaagaa ctactggaac accaagctca agaagaaact cctcatgtct 360
tcattaatgc tccctaatcc ttttgttagg cctcctaata atattaataa ctaccaacaa 420
tacctattat catcaccatc tccttcttca tattcctccc cattgcctcc aacattgaga 480
ctttgtgaca acaattaccc aaccctaggt gcttctaggt cattcacaag tgagggcttc 540
tcaaatattt caacaaacct ctttaatatc cagcctcaag atcagggttt gggtcccatg 600
caaaattatc aagagaaaga atcacctctc attatgtttg gaggggcagg tgatgatcag 660
caagctgcta gctctagttc ttatgatgaa catttcatga tgttctccaa ctgtgggatg 720
aataatattt ctgatcaaca gaagcaagta aatcttggtg tgtctagtgg tgatcatgag 780
attgttagca atgtactgga tcagtataac atgagctgtg ttgaggagat taagcaactg 840
attagcacta ctaatctgtc atgtaattct aatctgagtt tctttgttga tgaaaacaag 900
acagtgagaa gtgaggagga gaaagtgttg atgtactac 939
<210>97
<211>313
<212>PRT
<213>胡萝卜
<400>97
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Gln Lys Leu Lys Asp Tyr Ile Glu Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Arg Ile Ile Cys
65 70 75 80
Thr Leu Phe Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Gly Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Met Ser Ser Leu Met Leu Pro Asn Pro Phe
115 120 125
Val Arg Pro Pro Asn Asn Ile Asn Asn Tyr Gln Gln Tyr Leu Leu Ser
130 135 140
Ser Pro Ser Pro Ser Ser Tyr Ser Ser Pro Leu Pro Pro Thr Leu Arg
145 150 155 160
Leu Cys Asp Asn Asn Tyr Pro Thr Leu Gly Ala Ser Arg Ser Phe Thr
165 170 175
Ser Glu Gly Phe Ser Asn Ile Ser Thr Asn Leu Phe Asn Ile Gln Pro
180 185 190
Gln Asp Gln Gly Leu Gly Pro Met Gln Asn Tyr Gln Glu Lys Glu Ser
195 200 205
Pro Leu Ile Met Phe Gly Gly Ala Gly Asp Asp Gln Gln Ala Ala Ser
210 215 220
Ser Ser Ser Tyr Asp Glu His Phe Met Met Phe Ser Asn Cys Gly Met
225 230 235 240
Asn Asn Ile Ser Asp Gln Gln Lys Gln Val Asn Leu Gly Val Ser Ser
245 250 255
Gly Asp His Glu Ile Val Ser Asn Val Leu Asp Gln Tyr Asn Met Ser
260 26 5270
Cys Val Glu Glu Ile Lys Gln Leu Ile Ser Thr Thr Asn Leu Ser Cys
275 280 285
Asn Ser Asn Leu Ser Phe Phe Val Asp Glu Asn Lys Thr Val Arg Ser
290 295 300
Glu Glu Glu Lys Val Leu Met Tyr Tyr
305 310
<210>98
<211>744
<212>DNA
<213>油棕
<400>98
atgggaagag ccccttgttg cgacaaagcc aacgtgaaga aaggcccatg gtcccccgag 60
gaggacgcca agctaaaggc ctacatcgag cagtatggca ctggaggcaa ctggatcgcc 120
ctccctcaaa agattggcct taagcgatgc ggtaagagct gccgcctccg atggctaaac 180
tatcttcgac cgaatattaa gcatggagga ttctctgaag aagaagatca catcatttgt 240
agcctgtaca tcagcatcgg tagcagatgg tctataattg ccgcccaatt gccgggaagg 300
accgataacg acatcaagaa ctactggaac acaaggctga agaaaaagct tttaggcaag 360
caacgcaagg atcaccagca acaggcgcgt cgaggtgggg ggccaaagca agaagatagg 420
agtgatgaga gcggaaatcc ggcagttgcc ggcgagggtg gcagccggag cacgtattgg 480
cctgagccag ccatgcctgt ttactcgacc ggccagcccg atcatcatct caacaacaac 540
catgcttcga cgacagatag actgctgatg atgctcgggg gaaggtcctc caacgacagt 600
aatgcaagcc atcagagccc tttcattcca cagtactcat cagttccact gccacagatc 660
tataataatt cagctagctt aattaaccct tcggtcaaca ccacttcctt caaagaagcc 720
atgcaagttc ctcatcggtt tgca 744
<210>99
<211>248
<212>PRT
<213>油棕
<400>99
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Glu Gln Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp His Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Asp His Gln Gln Gln
115 120 125
Ala Arg Arg Gly Gly Gly Pro Lys Gln Glu Asp Arg Ser Asp Glu Ser
130 135 140
Gly Asn Pro Ala Val Ala Gly Glu Gly Gly Ser Arg Ser Thr Tyr Trp
145 150 155 160
Pro Glu Pro Ala Met Pro Val Tyr Ser Thr Gly Gln Pro Asp His His
165 170 175
Leu Asn Asn Asn His Ala Ser Thr Thr Asp Arg Leu Leu Met Met Leu
180 185 190
Gly Gly Arg Ser Ser Asn Asp Ser Asn Ala Ser His Gln Ser Pro Phe
195 200 205
Ile Pro Gln Tyr Ser Ser Val Pro Leu Pro Gln Ile Tyr Asn Asn Ser
210 215 220
Ala Ser Leu Ile Asn Pro Ser Val Asn Thr Thr Ser Phe Lys Glu Ala
225 230 235 240
Met Gln Val Pro His Arg Phe Ala
245
<210>100
<211>369
<212>DNA
<213>洲油棕榈
<400>100
atgggaagag caccgtgctg tgacaaggct aatgtgaaga gaggaccatg gtctccagag 60
gaagatgcag tccttaaaaa ctatattgag aagcatggga gtgttggtaa ctggattgct 120
ttgccccaaa aagcagggct caaacgttgc ggcaaaagct gccgcctccg atggctcaat 180
tacctccggc cagacatcaa gcacggcggc ttcacggagg aagaagacat cgccatctgc 240
actctctaca acaaaattgg aagcaggtgg tctgttattg cttccaagct gcgaggaaga 300
actgacaatg atgtcaagaa ttattggaac actaagctca agaaaaagat gataaacgga 360
caaattagt 369
<210>101
<211>123
<212>PRT
<213>洲油棕榈
<400>101
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Val Leu Lys Asn Tyr Ile Glu Lys His
20 25 30
Gly Ser Val Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Ile Ala Ile Cys
65 70 75 80
Thr Leu Tyr Asn Lys Ile Gly Ser Arg Trp Ser Val Ile Ala Ser Lys
85 90 95
Leu Arg Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Met Ile Asn Gly Gln Ile Ser
115 120
<210>102
<211>303
<212>DNA
<213>人参花
<400>102
atgggaagag ctccatgttg tgataaagca aatgtaaaga gaggtccatg gtcacctgaa 60
gaagatggaa agctcaaatc ctatattgaa gaacatggta ctggtggtaa ttggatagcc 120
ttgcctcaaa aaataggtct aaagagatgt gggaagagtt gtcgtctccg gtggttgaac 180
tatcttcgac caaacatcaa gcatggtggt ttctccgaag aagaagataa cattatatgc 240
aatctttata ttagtatcgg aagcaggtgg tctataatag cagcacaact acctggaaga 300
act 303
<210>103
<211>101
<212>PRT
<213>人参花
<400>103
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Gly Lys Leu Lys Ser Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr
100
<210>104
<211>332
<212>DNA
<213>乳浆大戟
<400>104
atggggagag ctccatgctg tgataaagct aatgtgaaga aaggtccatg gtcacctgag 60
gaagatgcta ctctcaaatc ttatattgaa gaaaatggca ccggcggcaa ctggattgcc 120
ttgcctcaaa aaattgggct taagagatgt gggaagagtt gtagacttag atggttgaat 180
tatctccggc caaatattaa acatggaggc ttttctgagg aggaagataa cattatttgc 240
agcctctata taaatattgg aagcaggtgg tctataattg ctgcacaatt acctggaagg 300
actgataatg atataaagaa ctactggaac ac 332
<210>105
<211>111
<212>PRT
<213>乳浆大戟
<400>105
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Thr Leu Lys Ser Tyr Ile Glu Glu Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr
100 105 110
<210>106
<211>771
<212>DNA
<213>乳浆大戟
<220>
<221>其他特征
<222>(747)..(747)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(748)..(748)
<223>n可以是任意核苷
<220>
<221>其他特征
<222>(749)..(749)
<223>n可以是任意核苷
<400>106
atgggaagag ctccttgttg tgataaggct aatgtgaaga aagggccatg gtctcctgag 60
gaagatgctg ctcttaagga ctatattgag aaaaatggaa ctggtggcaa ttggattgct 120
cttcctcaaa aagctggtct taaaagatgt ggaaaaagct gcagattaag atggctaaac 180
tatctaaggc ctaacatcaa gcatggagat ttttctgatc atgaagatag gatcatttgg 240
actctctact ccaacattgg aagcagatgg tcaataattg cagcacaatt accaggaaga 300
acagacaatg atataaaaaa ttattggaac acaaagttga agaagaagct aatgatgagc 360
ataatcaaca ctaatcctac tcttaatcct caaccaaaat tactatctac tgctggattt 420
tcttctcttc ttcaatttag tccttcttcc ccaatttctt ccaccacatc accatcatct 480
tcttcttctt cttcatcaat attaaacact aattacaata atttgtcatt agtagaaccc 540
acaattcagc cattttcaaa caacccgtta atgggttctc tccaaaatta tcctcatcaa 600
gcaaattatc atatgggttt tgaagggtat aatggtaatt actataatgg agagattaat 660
aatcacagtg agtatggatt agaggagatt aaggaaatga ttagcacaac taatacaatt 720
agtagcaact ttttgtttga agaaaannng acagagagta ttatgtactt t 771
<210>107
<211>257
<212>PRT
<213>乳浆大戟
<220>
<221>其他特征
<222>(249)..(249)
<223>X可以是任意氨基酸
<220>
<221>其他特征
<222>(250)..(250)
<223>X可以是任意氨基酸
<400>107
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Ala Leu Lys Asp Tyr Ile Glu Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp His Glu Asp Arg Ile Ile Trp
65 70 75 80
Thr Leu Tyr Ser Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Met Ser Ile Ile Asn Thr Asn Pro Thr Leu
115 120 125
Asn Pro Gln Pro Lys Leu Leu Ser Thr Ala Gly Phe Ser Ser Leu Leu
130 135 140
Gln Phe Ser Pro Ser Ser Pro Ile Ser Ser Thr Thr Ser Pro Ser Ser
145 150 155 160
Ser Ser Ser Ser Ser Ser Ile Leu Asn Thr Asn Tyr Asn Asn Leu Ser
165 170 175
Leu Val Glu Pro Thr Ile Gln Pro Phe Ser Asn Asn Pro Leu Met Gly
180 185 190
Ser Leu Gln Asn Tyr Pro His Gln Ala Asn Tyr His Met Gly Phe Glu
195 200 205
Gly Tyr Asn Gly Asn Tyr Tyr Asn Gly Glu Ile Asn Asn His Ser Glu
210 215 220
Tyr Gly Leu Glu Glu Ile Lys Glu Met Ile Ser Thr Thr Asn Thr Ile
225 230 235 240
Ser Ser Asn Phe Leu Phe Glu Glu Xaa Xaa Thr Glu Ser Ile Met Tyr
245 250 255
Phe
<210>108
<211>649
<212>DNA
<213>绿玉树
<400>108
aattattagt tcagcaagat caatacaagt atgggaagag ctccttgctg cgacaaagcc 60
aacgtcaaaa aaggcccttg gtcccctgaa gaagatagct aagcttaaat cttatattga 120
gaaacatggc accggtggta actggattgc tttgcctcaa aaaattggcc ttaaaagatg 180
tggaaagagt tgccgtctta gatagttgaa ctatcttcgc ccaaacatca aacatggcgg 240
cttctctgag gaagaagata acatcatttg cagcctttat attagtattg ggagcagata 300
gtctataatt gcagcccaat tgccaggaag aactgataat gacataaaaa attactggaa 360
cacaaggctg aaaaagaagc ttcttggaaa gcaaagaaaa gagcaacatt ctagcagaag 420
aggaaatggc ctcaagcaag atattatcaa gagagcaaat cggaccaata atattaattc 480
atctgatcaa aattactctc attggcctga gctgcctttg cttgctccta caccattcat 540
ctccacccaa aatcaagcct tcaatgatca tgcttctatc aggaaattgc ttgtgaagct 600
tggagggaaa ttttctgatg atcatcaaga taatcccatt tttaattct 649
<210>109
<211>671
<212>DNA
<213>银杏叶
<400>109
gattggattg ccttacccca taaagcaggt ctgaagcgat gcggtaagag ctgcagacta 60
agatggttga actatttgag gcctgatatc aagcatggag gcttctctga agaagaagac 120
actatcattt gtagcctcta cggcagcatt ggaagcaggt ggtctatcat tgctgcgcag 180
ttaccgggta gaacagacaa cgatatcaag aattactgga acacaagact gaagaaaaaa 240
ttgcttggga aatgcaagga tcatcagcaa actcgtcgtc tgtcaagaga agcagagaag 300
gaagtgagag cctttgcttc agaagccgtg tccgtggcca ccactccaac ctcgtctaca 360
tctcttgcaa atccagtatc ttatcacatg actactgatc cctctaatta tgccagtttg 420
gatgccggaa tggtctctca ggcgttggaa cactcgagtt ctcccataag aaccatattg 480
gctcatgatc agtctccatt atctccaagt acagctcatg ggtttgggaa tattcatgat 540
gatcacgaag ctttcagaga aatgtcagat tatgcctgcc ttacaagcct gctcatgcgg 600
cttaatgaag gcagcgattc tttcacaaac atttcttgcg gaatatcaag tacctctcaa 660
gttatatccc c 671
<210>110
<211>1068
<212>DNA
<213>黑大豆
<400>110
atggggaggg caccttgctg tgacaaagca aacgtgaaga aaggtccttg gtcgccagaa 60
gaagatacca aactcaaatc ctacatcgaa cagcacggaa ccggaggaaa ctggatcgct 120
ttgccacaaa agattggcct taaacgatgt ggcaagagtt gccgtctcag gtggctaaac 180
tacctccgcc ctaacatcag acacggtggt ttctccgaag aagaagacaa catcatttgc 240
agcctctacg ttagcattgg aagcaggtgg tcggtcattg cagcacaatt gccgggaaga 300
actgataatg acataaagaa ctattggaac acgaggctga agaagaagct tctagggaaa 360
caccgtaagg aactacaggc acgcaacaaa ggaaacggtg gtatccttaa acaggagaac 420
agttcctcgc tcttgcttca gcaaaacagt gctcaacaat tacatccatg ttggccacag 480
attccagtgc tgccactttc atcaccgtac acaaaccaaa gtccaagttt caacgaccaa 540
gattccatta gaaagcttct aatcaagctt ggagggagat tctccgatga ttatcaaccc 600
atcctagatg ggttgaatct tcagttccca catggttcaa attctctctc atcaacacaa 660
caaattcaag aggagcaagt ccatgttggt tcttctgcac tagggtgcat gaactctatt 720
ggccacaatc aagtacaatt tggtcagagt aatgagtact gtgctgagtt ggtgcaagga 780
caagggagtt tcattaccac agcaattggg gaaatggttt cttctaatga ttattctcga 840
aggttattag gtgggtcgtt ggagttcttc tatggggagg aaatgattac tgataataag 900
ataatgggtg cttgtgcttc ttcaagttgt gggcaaagta ctaattgggg tgaaaccagt 960
agttctctga tgtatccttc tcttgttgct tcgaattacg agggtgtgcg gcaagagatg 1020
ccacgagaat gtgcttttca agagttgagc tacccggggg cgcaatag 1068
<210>111
<211>355
<212>PRT
<213>黑大豆
<400>111
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys His Arg Lys Glu Leu Gln Ala Arg
115 120 125
Asn Lys Gly Asn Gly Gly Ile Leu Lys Gln Glu Asn Ser Ser Ser Leu
130 135 140
Leu Leu Gln Gln Asn Ser Ala Gln Gln Leu His Pro Cys Trp Pro Gln
145 150 155 160
Ile Pro Val Leu Pro Leu Ser Ser Pro Tyr Thr Asn Gln Ser Pro Ser
165 170 175
Phe Asn Asp Gln Asp Ser Ile Arg Lys Leu Leu Ile Lys Leu Gly Gly
180 185 190
Arg Phe Ser Asp Asp Tyr Gln Pro Ile Leu Asp Gly Leu Asn Leu Gln
195 200 205
Phe Pro His Gly Ser Asn Ser Leu Ser Ser Thr Gln Gln Ile Gln Glu
210 215 220
Glu Gln Val His Val Gly Ser Ser Ala Leu Gly Cys Met Asn Ser Ile
225 230 235 240
Gly His Asn Gln Val Gln Phe Gly Gln Ser Asn Glu Tyr Cys Ala Glu
245 250 255
Leu Val Gln Gly Gln Gly Ser Phe Ile Thr Thr Ala Ile Gly Glu Met
260 265 270
Val Ser Ser Asn Asp Tyr Ser Arg Arg Leu Leu Gly Gly Ser Leu Glu
275 280 285
Phe Phe Tyr Gly Glu Glu Met Ile Thr Asp Asn Lys Ile Met Gly Ala
290 295 300
Cys Ala Ser Ser Ser Cys Gly Gln Ser Thr Asn Trp Gly Glu Thr Ser
305 310 315 320
Ser Ser Leu Met Tyr Pro Ser Leu Val Ala Ser Asn Tyr Glu Gly Val
325 330 335
Arg Gln Glu Met Pro Arg Glu Cys Ala Phe Gln Glu Leu Ser Tyr Pro
340 345 350
Gly Ala Gln
355
<210>112
<211>444
<212>DNA
<213>黑大豆
<400>112
atggggaggg caccttgctg tgacaaagca aacgtgaaga aaggtccttg gtcgccagaa 60
gaagatacca aactcaaatc ctacatcgaa cagcacggaa ccggaggaaa ctggatcgct 120
ttgccacaaa agattggcct taaacgatgt ggcaagagtt gccgtctcag gtggctaaac 180
tacctccgcc ctaacatcag acacggtggt ttctccgaag aagaagacaa catcatttgc 240
agcctctacg ttagcattgg aagcaggtgg tcggtcattg cagcacaatt gccgggaaga 300
actgataatg acataaagaa ctattggaac acgaggctga agaagaagct tctagggaaa 360
caccgtaagg aactacaggc acgcaacaaa ggaaacggtg gtatccttaa acaggagaac 420
agttcctcgc tcttgcttca gcaa 444
<210>113
<211>148
<212>PRT
<213>黑大豆
<400>113
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Val Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys His Arg Lys Glu Leu Gln Ala Arg
115 120 125
Asn Lys Gly Asn Gly Gly Ile Leu Lys Gln Glu Asn Ser Ser Ser Leu
130 135 140
Leu Leu Gln Gln
145
<210>114
<211>627
<212>DNA
<213>黑大豆
<400>114
atgggtagag ctccatgctg tgacaaggcc aacgtgaaga aaggaccatg gtctcctgaa 60
gaggatgctg cactcaaagc ctacattgaa aagaatggaa ctggtggcaa ttggattgct 120
cttcctcaga aaattggttt ggaaaggtgt ggaaagagtt gcagacttag gtggttaaat 180
tacttgaggc ctaatatcaa acatggtgga tttactgaag aagaagacaa catcatctgc 240
agcctgtaca ttagcattgg aagcaggtgg tccataattg ctgctcagtt acctggaagg 300
acagataacg acatcaagaa ctattggaac actagattga agaagaaact gctcgggagg 360
cgcaaacaat ctaacttaag cgcaaaagac acaaacaatg gaatagagga gaattcttat 420
tcaaatgccc taagctcttc agctcttgaa agactccaac tgcacatgca acttcaaagc 480
cttcaaaacc ctttctcttt ctacaacaac ccggcgctgt ggcccaagtt gcatcctttc 540
caagaaaaga tgatccagag cctgcagtct ttgaatgatg gctctagccc tgtgatgcaa 600
aatgctttgc ctaattccca catgtcg 627
<210>115
<211>209
<212>PRT
<213>黑大豆
<400>115
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Ala Leu Lys Ala Tyr Ile Glu Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Glu
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Ser Asn Leu Ser Ala
115 120 125
Lys Asp Thr Asn Asn Gly Ile Glu Glu Asn Ser Tyr Ser Asn Ala Leu
130 135 140
Ser Ser Ser Ala Leu Glu Arg Leu Gln Leu His Met Gln Leu Gln Ser
145 150 155 160
Leu Gln Asn Pro Phe Ser Phe Tyr Asn Asn Pro Ala Leu Trp Pro Lys
165 170 175
Leu His Pro Phe Gln Glu Lys Met Ile Gln Ser Leu Gln Ser Leu Asn
180 185 190
Asp Gly Ser Ser Pro Val Met Gln Asn Ala Leu Pro Asn Ser His Met
195 200 205
Ser
<210>116
<211>684
<212>DNA
<213>黑大豆
<400>116
atgggtagag ctccttgttg tgacaaggca aatgtaaaga aagggccatg gtcaccagaa 60
gaagatgcaa agctaaagga ctacatagag caacatggca ctggaggcaa ttggattgcg 120
cttccacaaa aagttggttt gaaaagatgt ggaaagagtt gtagactaag gtggcttaat 180
tatcttagac ccaacattaa gcatggtcaa ttctctgaag cagaagataa aataatctgt 240
agcctctttg ctagcattgg aagcaggtgg tccataatag catctcagtt gccggggagg 300
actgacaatg atatcaagaa ctattggaac accaagctta agaagaaaat gatggccatg 360
aatccttcac tccaaaggaa gcctcaacaa attacccttt tatccatcct tcaaagttca 420
gcatattcat caccatcgtc attcagagac agtaacaaca tctcatacta ccaccctcac 480
ggttcctttt cctactcgtc aaatcttctg agtggtaaca acacttctgc ttctgctaat 540
tccttctttc aagcacaaga gagtttcatt gaccccactc agaagtgcca attcaaagat 600
agcagcaaca atagcatgtt tttgtttggt ggtgaagcca cagccacagc cactagttgc 660
agctcttctg atgggagctg caac 684
<210>117
<211>228
<212>PRT
<213>黑大豆
<400>117
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Val Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gln Phe Ser Glu Ala Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Met Met Ala Met Asn Pro Ser Leu Gln Arg Lys Pro
115 120 125
Gln Gln Ile Thr Leu Leu Ser Ile Leu Gln Ser Ser Ala Tyr Ser Ser
130 135 140
Pro Ser Ser Phe Arg Asp Ser Asn Asn Ile Ser Tyr Tyr His Pro His
145 150 155 160
Gly Ser Phe Ser Tyr Ser Ser Asn Leu Leu Ser Gly Asn Asn Thr Ser
165 170 175
Ala Ser Ala Asn Ser Phe Phe Gln Ala Gln Glu Ser Phe Ile Asp Pro
180 185 190
Thr Gln Lys Cys Gln Phe Lys Asp Ser Ser Asn Asn Ser Met Phe Leu
195 200 205
Phe Gly Gly Glu Ala Thr Ala Thr Ala Thr Ser Cys Ser Ser Ser Asp
210 215 220
Gly Ser Cys Asn
225
<210>118
<211>1068
<212>DNA
<213>棉花属
<400>118
atggggagag ctccttgttg tgacaaagct aacgtcaaga aaggcccatg gtcacctgaa 60
gaagacacca agctcaaggc atacatcgag cagcatggca ctggcggaaa ctggatcgct 120
ttgcctcaca aaattggcct taagagatgt gggaagagct gccgcctcag atggttaaac 180
tatctccgcc caaatattaa gcatggagga ttctccgaag aagaagataa aattatttgc 240
agcctctata tcagtattgg gagcaggtgg tctattattg ctgcacaatt accggggagg 300
actgataacg atataaagaa ctattggaac acaaggctta agaagaagct tctgggcaag 360
caacgcaaag agcatcagtc tcgaagaggc aacagcctaa agcaagatat gaagagatca 420
tcagctagtg tgggggattc catggttcct gcagataaca tcaatcaaat cccttactgg 480
ccagagctgc ctgtgctggc tgcggcggcg gctcccatac cgcactcaag tcaagaacat 540
cgcattgaca gccaagcctc gatgagaaga ttactaatca agcttggggg aagattttct 600
gaggatgatc atgtggttaa tgatgggaca actcttcatc agtttcctaa tgatttatcc 660
actactgatc aggatcttta cgagcagact gtctatgtgc cctcttcttc ttcttcttct 720
cccatggatg ccttgagctt aagcaataac atcggttctc agtttgtgaa ctctcagttc 780
gccatagatg gagggaatct gcccattctg caaggacaaa gcactacttt ttcatcagag 840
ctccaggaaa tgggatatag cagcaaccca cagagattag atggaatgga gttcttatat 900
ggggaaggca tggtcgataa tagaggcgtg aatccttgtg aaagcattgg ctggggtgac 960
actagctctc tggttggccc tccttgtgct tcggaatatg gagtcatgca acaaggaatg 1020
cttcaagaat atggttttag tgagatgagg tacccaggag gagcgcaa 1068
<210>119
<211>356
<212>PRT
<213>棉花属
<400>119
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Lys Leu Lys Ala Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu His Gln Ser Arg
115 120 125
Arg Gly Asn Ser Leu Lys Gln Asp Met Lys Arg Ser Ser Ala Ser Val
130 135 140
Gly Asp Ser Met Val Pro Ala Asp Asn Ile Asn Gln Ile Pro Tyr Trp
145 150 155 160
Pro Glu Leu Pro Val Leu Ala Ala Ala Ala Ala Pro Ile Pro His Ser
165 170 175
Ser Gln Glu His Arg Ile Asp Ser Gln Ala Ser Met Arg Arg Leu Leu
180 185 190
Ile Lys Leu Gly Gly Arg Phe Ser Glu Asp Asp His Val Val Asn Asp
195 200 205
Gly Thr Thr Leu His Gln Phe Pro Asn Asp Leu Ser Thr Thr Asp Gln
210 215 220
Asp Leu Tyr Glu Gln Thr Val Tyr Val Pro Ser Ser Ser Ser Ser Ser
225 230 235 240
Pro Met Asp Ala Leu Ser Leu Ser Asn Asn Ile Gly Ser Gln Phe Val
245 250 255
Asn Ser Gln Phe Ala Ile Asp Gly Gly Asn Leu Pro Ile Leu Gln Gly
260 265 270
Gln Ser Thr Thr Phe Ser Ser Glu Leu Gln Glu Met Gly Tyr Ser Ser
275 280 285
Asn Pro Gln Arg Leu Asp Gly Met Glu Phe Leu Tyr Gly Glu Gly Met
290 295 300
Val Asp Asn Arg Gly Val Asn Pro Cys Glu Ser Ile Gly Trp Gly Asp
305 310 315 320
Thr Ser Ser Leu Val Gly Pro Pro Cys Ala Ser Glu Tyr Gly Val Met
325 330 335
Gln Gln Gly Met Leu Gln Glu Tyr Gly Phe Ser Glu Met Arg Tyr Pro
340 345 350
Gly Gly Ala Gln
355
<210>120
<211>633
<212>DNA
<213>棉花属
<400>120
atgggtcgag ctccttgctg tgacaaagcc aacgtgaaga aaggaccatg gtcacctgaa 60
gaagatgcta agcttaaggc ttatatcgaa cagtatggca ctggtggcaa ctggattgct 120
cttcctcaga aaattggtct taagcgatgt gggaagagtt gcaggttgag atggctgaat 180
tatttgaggc caaacattaa gcatggtgga ttctctgagg aagaagacaa catcatatgc 240
agtctctata taagtatagg aagcaggtgg tccataattg ctgcacaact gcctggaaga 300
actgataatg atatcaaaaa ctactggaac accaaattaa agaagaaact acttgggagg 360
cgcaaacaac ctagtaacat ccataggtta tcgaatcaag accctaatga tcctcatcaa 420
cctactggtt cagatgataa tcaattctct cagggtttga gtaacttagc catggaacgt 480
cttcagcttc atatgcagct ccaaactctt cagaaccctt tctctttcta taataaccct 540
gctttatggc ctaagattca ccccttgcaa gaaaagatga tccaaaacat gcaggcttct 600
aatggaaacc cttgtctcgt cctctgcctg atg 633
<210>121
<211>211
<212>PRT
<213>棉花属
<400>121
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Glu Gln Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Pro Ser Asn Ile His
115 120 125
Arg Leu Ser Asn Gln Asp Pro Asn Asp Pro His Gln Pro Thr Gly Ser
130 135 140
Asp Asp Asn Gln Phe Ser Gln Gly Leu Ser Asn Leu Ala Met Glu Arg
145 150 155 160
Leu Gln Leu His Met Gln Leu Gln Thr Leu Gln Asn Pro Phe Ser Phe
165 170 175
Tyr Asn Asn Pro Ala Leu Trp Pro Lys Ile His Pro Leu Gln Glu Lys
180 185 190
Met Ile Gln Asn Met Gln Ala Ser Asn Gly Asn Pro Cys Leu Val Leu
195 200 205
Cys Leu Met
210
<210>122
<211>542
<212>DNA
<213>Hedyotis terminalis
<400>122
atgggaagag caccttgctg cgacaaagcc aatgtgaagc gaggtccatg gtcacctgaa 60
gaagatgcta aactcaaatc ctacattgag gaatttggca ccggaggaaa ttggatttcc 120
ttaccccaga aaataggtct caagagatgc ggaaaaagtt gtcggctgag atggttgaat 180
tatctgcggc ctaacatcaa gcatggcgga ttttcagaag aagaagatac cataatctgc 240
agcctctata tgagcatagg aagcaggtgg tctattatag cgggacagct gcccggaaga 300
actgataatg acataaagaa ctactggaat acgaagctga agaagaagtt actagggaaa 360
caacgaaagg atccaaagcc acgttcatca agctcagtct tgactaaaag aaaacagaat 420
tcagtaattt ctgaagacat aagtttatac aacaccataa cacatccaca gatggcttta 480
ccagcagttc atgttccaaa gcttgaacaa caagtccatt tccagtatca gccatctttt 540
gc 542
<210>123
<211>181
<212>PRT
<213>Hedyotis terminalis
<400>123
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Glu Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ser Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Thr Ile Ile Cys
65 70 75 80
Ser Leu Tyr Met Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Gly Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Asp Pro Lys Pro Arg
115 120 125
Ser Ser Ser Ser Val Leu Thr Lys Arg Lys Gln Asn Ser Val Ile Ser
130 135 140
Glu Asp Ile Ser Leu Tyr Asn Thr Ile Thr His Pro Gln Met Ala Leu
145 150 155 160
Pro Ala Val His Val Pro Lys Leu Glu Gln Gln Val His Phe Gln Tyr
165 170 175
Gln Pro Ser Phe Ala
180
<210>124
<211>616
<212>DNA
<213>向日葵
<400>124
gatgcaaaac ttaaagaatt tattgagaaa tacggaactg gtggtaactg gattgctctc 60
cctcaaaaag ctggtcttaa aagatgtgga aagagttgca ggttaagatg gctaaactat 120
ctgagaccaa atataagaca tggtgaattc tctgaagagg aagataggat cattttcagc 180
ttgtatgcta gcattggtag cagatggtcg gtaatagcag ctcagctacc aggaaggact 240
gacaatgaca tcaagaatta ctggaacact aaactgaaga agaagctttt aactatgctt 300
ccttattttc aagaaaaatc atcttttttt ccacctatac cctttcaacc acaaccacca 360
tcaaaggatc atcttacatc tcaccagttc ttgaacaatt catcatcatt ctacactttt 420
agtaccccta acttcatgaa tgttgacaac acttctaact ctctctttgt tcatgaagag 480
catactaatg ttactgatct tcagtcatca gtaccacccc cacccccacc actacaactt 540
catgcaaaca gtttgatgtg caacaacaat actgatgtta atgataactg ttatagtagt 600
tatagcatgt gcaaca 616
<210>125
<211>645
<212>DNA
<213>绢毛葵
<400>125
atgggtagag caccatgttg tgacaaaaca aatgtgaaaa agggaccttg gtcttctgaa 60
gaagatgcta agctcaaaga ttacatcgaa aagtatggta ctggtggtaa ctggattgca 120
cttccccaga agatcgggct aaagaggtgt ggaaaaagtt gcagattgag atggttaaat 180
taccttcgac ccaacatcaa gcatggagga ttctctgaag aagaagacaa catcatctgc 240
agcctctata ttagtatagg cagcaggtgg tctataatag cggctcaact tcctggaaga 300
acagataacg acatcaagaa ctactggaac acaaggctga agaagaagtt actcggaagg 360
cgcaaacaat cgcaggcgaa taagctctcg ggttcaagcc agtacccgaa agacggcagc 420
ggaatagaaa ccctaagcaa ctcagctatt gaaaggcttc aactccacat gcaactccaa 480
agccttgaaa accctaattt ccctcactat gataatcccc ccatgtggcc ttgcaaattg 540
aatcctatac aagaaaaaat gatgcaaact ctccaacttg caaatgaatc ctctaatcct 600
ctcatgatgc aaaatttctc acctgctact cctcaaaagg ttgaa 645
<210>126
<211>215
<212>PRT
<213>绢毛葵
<400>126
Met Gly Arg Ala Pro Cys Cys Asp Lys Thr Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Ser Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Ser Gln Ala Asn Lys
115 120 125
Leu Ser Gly Ser Ser Gln Tyr Pro Lys Asp Gly Ser Gly Ile Glu Thr
130 135 140
Leu Ser Asn Ser Ala Ile Glu Arg Leu Gln Leu His Met Gln Leu Gln
145 150 155 160
Ser Leu Glu Asn Pro Asn Phe Pro His Tyr Asp Asn Pro Pro Met Trp
165 170 175
Pro Cys Lys Leu Asn Pro Ile Gln Glu Lys Met Met Gln Thr Leu Gln
180 185 190
Leu Ala Asn Glu Ser Ser Asn Pro Leu Met Met Gln Asn Phe Ser Pro
195 200 205
Ala Thr Pro Gln Lys Val Glu
210 215
<210>127
<211>716
<212>DNA
<213>绢毛葵
<400>127
atggggagag ccccttgttg tgacaaagca aatgtgaaga aggggccatg gtcttctgaa 60
gaagatgcaa aactcaaaga atttattggg aaatatggaa ctggtgggaa ctggattgct 120
ctccctcaaa aagctggcct taaaagatgt ggaaagagtt gcagattaag gtggctaaac 180
tatctaagac ccaacattag acatggtgaa tactctgatg aagaagacag gatcatatgc 240
aacttgtatg ctagcattgg tagcagatgg tctgttatag ctgctcaact accaggaagg 300
actgataatg atatcaagaa ctactggaac accaaactga agaagaaact tttaactatg 360
ccttcttacc ttcaagaaaa acaaacattt ttttcatcta tgtcctttca accaccacca 420
ccatcatata atattacatc acaccaattg ttaaacaatt catcatcatg ttactctttt 480
agtaccccta acttcatgaa tgttgacaac atgtccaact ctctctttgt taatgatgat 540
attcataaca atcttactaa tcttcaatca ccaacacctc ttgcaaacaa tttggtgagt 600
aacaacagtg ttgatgttgt taatgataac tgtttgagtt atctagggtt tcaagatggg 660
cattatggca tgtgcaatag tccaatgctc atgtatggag gtacatgttc ttcttc 716
<210>128
<211>239
<212>PRT
<213>绢毛葵
<400>128
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Ser Glu Glu Asp Ala Lys Leu Lys Glu Phe Ile Gly Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Glu Tyr Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Asn Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Thr Met Pro Ser Tyr Leu Gln Glu Lys Gln
115 120 125
Thr Phe Phe Ser Ser Met Ser Phe Gln Pro Pro Pro Pro Ser Tyr Asn
130 135 140
Ile Thr Ser His Gln Leu Leu Asn Asn Ser Ser Ser Cys Tyr Ser Phe
145 150 155 160
Ser Thr Pro Asn Phe Met Asn Val Asp Asn Met Ser Asn Ser Leu Phe
165 170 175
Val Asn Asp Asp Ile His Asn Asn Leu Thr Asn Leu Gln Ser Pro Thr
180 185 190
Pro Leu Ala Asn Asn Leu Val Ser Asn Asn Ser Val Asp Val Val Asn
195 200 205
Asp Asn Cys Leu Ser Tyr Leu Gly Phe Gln Asp Gly His Tyr Gly Met
210 215 220
Cys Asn Ser Pro Met Leu Met Tyr Gly Gly Thr Cys Ser Ser Ser
225 230 235
<210>129
<211>803
<212>DNA
<213>蓝茎向日葵
<400>129
agactacata caaactcatg gtactggtgg caactggatt actctcccac aaaaagcagg 60
ccttagaaga tgtggtaaga gttgcaggtt gagatggctc aactatctca gaccaaacat 120
caaacatggt gaattttctg atgatgaaga caaacttatt tgtacccttt atgctagcat 180
tggtagcagg tggtcaataa tggcagcaca gttaccagga agaacagata atgatatcaa 240
gaactactgg aacacaaaac tgaagaagaa gaagatgatg agcaacttaa ttaccatgcc 300
tgaaattcaa aaacctcttc atcatcttca ttctttcatt tcctcgacaa actacaacta 360
cccatcactg tcaaatttcc atcctagtga tatcaatatt aatgctccat tatcatcacc 420
accaccacca cagtcatcat atatctatag ctcacacact gctagatatc atgatcaaac 480
cctatctatc ccatcaagtc ctacaataac aacaactgct tctgctgatg ctgctgctgt 540
tgtttctgat catcatcacc atccagttct acaaccacaa gatcatggga atcttggtta 600
ccaaagggta aaagacagtt ctacccttgt gatgtttgga ggtgatcaag ctagttccag 660
tacttctaac tctgagggga gttgtcatta tgagtatggt actggtatag gtgtgtatga 720
tcacaacaag tacaacatgg gacatatgag gagcaatcct ttcaaggaat gtgggatgat 780
gacacatgat aaggattttg ggt 803
<210>130
<211>684
<212>DNA
<213>线叶旋复花(具柄向日葵)
<220>
<221>其他特征
<222>(631)..(631)
<223>n是任意核苷
<400>130
atgggaagag cactttgttg tgacaaagct aatgtcaaaa gaggaccatg gtcacctgaa 60
gaagatgcca aactcaagtc atatatcgat gaatatggca ccggcggcaa ctggattgcg 120
ctaccgcata aaataggact caggagatgt ggcaagagtt gtcgccttcg atggttaaac 180
tatcttcgtc cgaatatcaa gcatggatct ttctcggaag aagaagacca tatcatttcc 240
accctctatc tcaatatcgg tagccggtgg tccattatag cttcacaatt acccgggaga 300
actgataatg atatcaagaa ctactggaac acaagactaa agaaaaaact catgggaatg 360
cagcgaaaag atcaacaatt gtcaaagaga ggtgggataa tcaagcaaga aatgaagaga 420
gaggttcttg aagatttaaa ggtaccatct ttatccacaa gcatgaatct ttatcaatct 480
ttctggccta cagaattccc tttgatagtc acaaacccta accatcatgg tcaagaactt 540
catgtgaaac cacaagatcc cacctttaat cactaccctt ttgacacaac ttcaatccaa 600
ccagaattgt tggatcaaaa caacaagagt ncattgccaa acacacccta ttttgctaat 660
ggggatgttg taaacctatt tcaa 684
<210>131
<211>228
<212>PRT
<213>线叶旋复花(具柄向日葵)
<220>
<221>其他特征
<222>(211)..(211)
<223>X是任意氨基酸
<400>131
Met Gly Arg Ala Leu Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Asp Glu Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Ser
65 70 75 80
Thr Leu Tyr Leu Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Met Gly Met Gln Arg Lys Asp Gln Gln Leu Ser
115 120 125
Lys Arg Gly Gly Ile Ile Lys Gln Glu Met Lys Arg Glu Val Leu Glu
130 135 140
Asp Leu Lys Val Pro Ser Leu Ser Thr Ser Met Asn Leu Tyr Gln Ser
145 150 155 160
Phe Trp Pro Thr Glu Phe Pro Leu Ile Val Thr Asn Pro Asn His His
165 170 175
Gly Gln Glu Leu His Val Lys Pro Gln Asp Pro Thr Phe Asn His Tyr
180 185 190
Pro Phe Asp Thr Thr Ser Ile Gln Pro Glu Leu Leu Asp Gln Asn Asn
195 200 205
Lys Ser Xaa Leu Pro Asn Thr Pro Tyr Phe Ala Asn Gly Asp Val Val
210 215 220
Asn Leu Phe Gln
225
<210>132
<211>654
<212>DNA
<213>线叶旋复花(具柄向日葵)
<400>132
atggggagag ccccttgttg tgacaaagca aatgtgaaga aagggccatg gtcttctgaa 60
gaagatgcaa aactcaaaga atttattggg aaatatggaa ctggtgggaa ctggattgct 120
ctccctcaaa aagctggcct taaaagatgt ggaaagagtt gcagattaag gtggctaaac 180
tatctaagac ccaacattag acatggtgaa tactctgatg aagaagacag gatcatatgc 240
aacttgtatg ctagcattgg tagcagatgg tctgttatag ctgctcaact accaggaagg 300
actgataatg atatcaagaa ttactggaac actaaactga agaagaaact tttaactatg 360
ccttcttacc ttcaagaaaa gcaaacattt ttttcatcca tgcccttcca accaccacca 420
ccatcatata atattacatc acaccagatc ttgaacaatt catcatcatg ttactctttt 480
agtaccccta acttcatgaa tgttgacaac atgtccaact cactctttgt tcatgatgat 540
attcataaca atcttactaa tcttcaatca ccaacacctc ttgcagacaa tttgatgagt 600
aacaacagtg ttgatgttgt taatgataag tgtttgagtt atctagggtt tcaa 654
<210>133
<211>218
<212>PRT
<213>线叶旋复花(具柄向日葵)
<400>133
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Ser Glu Glu Asp Ala Lys Leu Lys Glu Phe Ile Gly Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Glu Tyr Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Asn Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Thr Met Pro Ser Tyr Leu Gln Glu Lys Gln
115 120 125
Thr Phe Phe Ser Ser Met Pro Phe Gln Pro Pro Pro Pro Ser Tyr Asn
130 135 140
Ile Thr Ser His Gln Ile Leu Asn Asn Ser Ser Ser Cys Tyr Ser Phe
145 150 155 160
Ser Thr Pro Asn Phe Met Asn Val Asp Asn Met Ser Asn Ser Leu Phe
165 170 175
Val His Asp Asp Ile His Asn Asn Leu Thr Asn Leu Gln Ser Pro Thr
180 185 190
Pro Leu Ala Asp Asn Leu Met Ser Asn Asn Ser Val Asp Val Val Asn
195 200 205
Asp Lys Cys Leu Ser Tyr Leu Gly Phe Gln
210 215
<210>134
<211>840
<212>DNA
<213>Helianthus paradoxus
<400>134
tgccaactaa ggcttatatc gaagaacatg gcaccggagg caactggatt gctctacctc 60
ataaaattgg cctcaagaga tgtggcaaga gttgtcgcct tcgatggcta aactatcttc 120
gcccaaacat caagcatgga tctttctccg aggaagaaga tcacatcatt tgcaccctct 180
atctcagtat tggcagccgg tggtctatca tagcggcaca attgcctggg cgaacggata 240
atgatatcaa gaactactgg aacacgagac taaagaaaaa actaatggga aaacaacgga 300
aagatcaaca atcgtcgaaa cgaggttgct taatcaagca agaaatgaag agagaggttc 360
ttgaagattt aaaggcacca tctttatgca tgagcatgaa tctttatcaa tcttactggc 420
caacagaatt ccctttgatt ctcacaaacc ctaatcagca tcatcatcaa gaacttgcca 480
aaacccaaga tcccactttt gagccatacc aatttgacac aatctcaaat caacccgaat 540
tgttcaatca aaacaacgat aaaccgatat caactgcttc ttaccatcct cagttgccaa 600
acacacccta ttttctaaat ggagatggta taaatctgtt tcaagaattc aacaattacc 660
catttgggat cgatgaattt ggctgcaaca caacacagtt agagcagttt gatgggttgg 720
ttgggggttt ggataacctt gtcaatggaa gcaattgcac aagctcatcg gcagaaagta 780
caactagttg gggtgatcct ctagcatgcc ctcctccccc tccgatggcg tatgatgatt 840
<210>135
<211>429
<212>DNA
<213>Helianthus petiolaris(向日葵的一种)
<400>135
atgggaagag caccttgctg tgacaaagcc aacgtcaaaa gaggaccatg gtcacctgaa 60
gaagatgcca aactaaaggc ttatatcgaa gaacatggca ccggaggcaa ctggattgct 120
ctacctcata aaattggcct caagagatgt ggcaagagtt gtcgccttcg atggctaaac 180
tatcttcgcc caaacatcaa gcatggatct ttctccgagg aagaagatca catcatttgc 240
accctctatc tcagtattgg cagccggtgg tctatcatag cggcacaatt gcctgggcga 300
acggataatg atatcaagaa ctactggaac acgagactaa agaaaaaaac taatgggaaa 360
acaacggaaa gatcaacaat cgtcgaaacg aggtggctta atcaagcaag aaatgaagag 420
agaggttct 429
<210>136
<211>143
<212>PRT
<213>Helianthus petiolaris(向日葵的一种)
<400>136
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Cys
65 70 75 80
Thr Leu Tyr Leu Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Thr Asn Gly Lys Thr Thr Glu Arg Ser Thr Ile Val
115 120 125
Glu Thr Arg Trp Leu Asn Gln Ala Arg Asn Glu Glu Arg Gly Ser
130 135 140
<210>137
<211>723
<212>DNA
<213>Helianthus petiolaris(向日葵的一种)
<400>137
atgggaagag caccttgttg tgacaaagct aatgtaaaaa gaggaccatg gtcacctgaa 60
gaagatgcca aactcaagtc atatattgat gaatatggca ccggcggcaa ctggatcgcg 120
ctaccgcata aaataggact caggagatgt ggcaagagtt gccgccttcg atggttaaac 180
tatcttcgtc cgaatatcaa gcatggatct ttctcggaag aagaagatca tatcatttcc 240
actctctatc tcaatatcgg tagccggtgg tcaattatag cttcacattt acccggccgg 300
actgataatg atatcaagaa ctactggaac acaagactaa agaaaaaact catgggaatg 360
cagcgaaaag atcaacaatt gtcaaagaga ggggggatag tcaagcaaga aatgaagaga 420
gaggttcttg aagaattaaa gggaccatct ttagctacaa gcatgaatct ttatcaatct 480
ttttggccta cagaatccgc tttgatagtc acaaacccta atcatcatgg tcaagaactt 540
catgtgaaac cacaagatcc cacctttaat cactaccctt ttgacacaac ctcaatccaa 600
ccagaattgt tggatcaaaa caacaagaat cagttgctaa acacacccta ttttgctaat 660
ggggatgttg taaacctatt tcaagaattc acaattaccc atttgagatc aatgagttta 720
act 723
<210>138
<211>241
<212>PRT
<213>Helianthus petiolaris(向日葵的一种)
<400>138
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Asp Glu Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Ser
65 70 75 80
Thr Leu Tyr Leu Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser His
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Met Gly Met Gln Arg Lys Asp Gln Gln Leu Ser
115 120 125
Lys Arg Gly Gly Ile Val Lys Gln Glu Met Lys Arg Glu Val Leu Glu
130 135 140
Glu Leu Lys Gly Pro Ser Leu Ala Thr Ser Met Asn Leu Tyr Gln Ser
145 150 155 160
Phe Trp Pro Thr Glu Ser Ala Leu Ile Val Thr Asn Pro Asn His His
165 170 175
Gly Gln Glu Leu His Val Lys Pro Gln Asp Pro Thr Phe Asn His Tyr
180 185 190
Pro Phe Asp Thr Thr Ser Ile Gln Pro Glu Leu Leu Asp Gln Asn Asn
195 200 205
Lys Asn Gln Leu Leu Asn Thr Pro Tyr Phe Ala Asn Gly Asp Val Val
210 215 220
Asn Leu Phe Gln Glu Phe Thr Ile Thr His Leu Arg Ser Met Ser Leu
225 230 235 240
Thr
<210>139
<211>758
<212>DNA
<213>洋姜
<220>
<221>其他特征
<222>(612)..(612)
<223>n是任意核苷
<400>139
atgggtagag caccatgttg tgacaaaaca aatgtgaaaa agggaccttg gtcttctgaa 60
gaagatgcta agctcaaaga ttatatcgaa aagtatggta ctggtggtaa ctggattgca 120
cttccccaga agatcgggct aaagaggtgc ggaaaaagtt gcagattgag atggttaaat 180
taccttcgac ccaacatcaa gcatggagga ttctctgaag aagaagacaa catcatctgc 240
agcctctata ttagtatagg cagcaggtgg tctataatag cggctcaact tcctggaaga 300
acagataacg acatcaagaa ctactggaac acaaggttga agaagaagtt actcggaagg 360
cgcaaacaat cacaggcgaa taagctttcg ggttcaagcc aggacccgaa agacggcagc 420
ggaatagaaa ccctaagcaa ctcagccatt gaaaggcttc aacttcacat gcaactccaa 480
agccttgaaa accctaattt ccctcactat gataatcccc ccatgtggcc ttgcaagttg 540
aatcctatac aagaaaaaat gatgcaaact ctccaacttg caaatgaagc ctctaaccct 600
ctcatgatgc anaatttctc acctgctact cctcaaaaag ttgaatacta tgcacaatcc 660
aataaccctc tttctctaca agaaaactgg gtcgtttccg gcaatatgat gattaacgga 720
atggagaatt ccatgggaat caacattcct gagtcatc 758
<210>140
<211>253
<212>PRT
<213>洋姜
<220>
<221>其他特征
<222>(204)..(204)
<223>X是任意氨基酸
<400>140
Met Gly Arg Ala Pro Cys Cys Asp Lys Thr Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Ser Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Ser Gln Ala Asn Lys
115 120 125
Leu Ser Gly Ser Ser Gln Asp Pro Lys Asp Gly Ser Gly Ile Glu Thr
130 135 140
Leu Ser Asn Ser Ala Ile Glu Arg Leu Gln Leu His Met Gln Leu Gln
145 150 155 160
Ser Leu Glu Asn Pro Asn Phe Pro His Tyr Asp Asn Pro Pro Met Trp
165 170 175
Pro Cys Lys Leu Asn Pro Ile Gln Glu Lys Met Met Gln Thr Leu Gln
180 185 190
Leu Ala Asn Glu Ala Ser Asn Pro Leu Met Met Xaa Asn Phe Ser Pro
195 200 205
Ala Thr Pro Gln Lys Val Glu Tyr Tyr Ala Gln Ser Asn Asn Pro Leu
210 215 220
Ser Leu Gln Glu Asn Trp Val Val Ser Gly Asn Met Met Ile Asn Gly
225 230 235 240
Met Glu Asn Ser Met Gly Ile Asn Ile Pro Glu Ser Ser
245 250
<210>141
<211>473
<212>DNA
<213>元麦
<400>141
atggggaggg cgccgtgctg cgacagggcg gcggtgaacc ggggcccgtg gtcgccggag 60
gaggacgacg cgctgcgcga ctacatgcag cgccatggca acaccggcag ctggatcacc 120
ctccccgcca aagccgggct caagaggtgc ggcaagagct gcaggctgcg gtggctcaac 180
tacctgcgcc cggacatccg ccacggcggc ttcaccgacg aggaggacgc catcatctac 240
tccctctaca gccagctcgg cagcaagtgg tcgctgatag cgtcgcagct ggagaggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agaagcgcct cgtcgccgcg 360
gccgccgcct tcccggccgc cccctcctcc cgccgccccc cgtccttcac tccggccgca 420
gcgcacgcgc acgcgcaccc gtcgccgctg ctcccgctcc ccgcgccgac cgt 473
<210>142
<211>158
<212>PRT
<213>元麦
<400>142
Met Gly Arg Ala Pro Cys Cys Asp Arg Ala Ala Val Asn Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Asp Ala Leu Arg Asp Tyr Met Gln Arg His
20 25 30
Gly Asn Thr Gly Ser Trp Ile Thr Leu Pro Ala Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Asp Glu Glu Asp Ala Ile Ile Tyr
65 70 75 80
Ser Leu Tyr Ser Gln Leu Gly Ser Lys Trp Ser Leu Ile Ala Ser Gln
85 90 95
Leu Glu Arg Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Val Ala Ala Ala Ala Ala Phe Pro Ala Ala Pro
115 120 125
Ser Ser Arg Arg Pro Pro Ser Phe Thr Pro Ala Ala Ala His Ala His
130 135 140
Ala His Pro Ser Pro Leu Leu Pro Leu Pro Ala Pro Thr Val
145 150 155
<210>143
<211>792
<212>DNA
<213>啤酒花
<400>143
atgggaagag ccccttgttg cgacaaaacc aaagtgaaaa gaggaccttg gtctcctgaa 60
gaagacgccg ccctcaagca ctacatgcac aacaatggaa ctgggggtaa ttggattgct 120
ctacctcaca aagcaggtct taaccggtgc ggcaaaagtt gtcgtttgag atggctgaat 180
tatctcagac cagatatcaa gcacgggggt tttaccgagg aagaagacaa tgttgtttgg 240
accctttaca gtaacatcgg aagtaggtgg tctgtcatag catcccaact acctggaaga 300
acagacaacg atgtgaaaaa ccactggaac accaagttga agaaaaaact attggcaaga 360
agcaccaact gcaatgagac tactaacact accgatcacc tcaacttggc tcggttctca 420
ccattgattc cgaaaactga gaattctgat caagggaact cagcttattg ttatactaat 480
tcagatacat taccatatct gatgaatggg aactgtgtgc aaaactttga tctcaagaag 540
ccaagtaaac cttcttactc atcaaaccct gttcaagttt ctgattttgg aacaaatggg 600
aacaccagtt ctagcatttc atcaactaga gaggcttcga gtctttcaac ttcatcatct 660
actttggcta tggagaacta tactaatttt gcttcatggt ctggcactgg cattgaaagt 720
actgaagatg ggattcttgt ggacttggga tttgactctt ctaatgatgt tttcctgagt 780
ggctttggat tt 792
<210>144
<211>264
<212>PRT
<213>啤酒花
<400>144
Met Gly Arg Ala Pro Cys Cys Asp Lys Thr Lys Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Ala Leu Lys His Tyr Met His Asn Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Asn Val Val Trp
65 70 75 80
Thr Leu Tyr Ser Asn Ile Gly Ser Arg Trp Ser Val Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Ala Arg Ser Thr Asn Cys Asn Glu Thr Thr
115 120 125
Asn Thr Thr Asp His Leu Asn Leu Ala Arg Phe Ser Pro Leu Ile Pro
130 135 140
Lys Thr Glu Asn Ser Asp Gln Gly Asn Ser Ala Tyr Cys Tyr Thr Asn
145 150 155 160
Ser Asp Thr Leu Pro Tyr Leu Met Asn Gly Asn Cys Val Gln Asn Phe
165 170 175
Asp Leu Lys Lys Pro Ser Lys Pro Ser Tyr Ser Ser Asn Pro Val Gln
180 185 190
Val Ser Asp Phe Gly Thr Asn Gly Asn Thr Ser Ser Ser Ile Ser Ser
195 200 205
Thr Arg Glu Ala Ser Ser Leu Ser Thr Ser Ser Ser Thr Leu Ala Met
210 215 220
Glu Asn Tyr Thr Asn Phe Ala Ser Trp Ser Gly Thr Gly Ile Glu Ser
225 230 235 240
Thr Glu Asp Gly Ile Leu Val Asp Leu Gly Phe Asp Ser Ser Asn Asp
245 250 255
Val Phe Leu Ser Gly Phe Gly Phe
260
<210>145
<211>783
<212>DNA
<213>宿根莴苣
<220>
<221>其他特征
<222>(709)..(709)
<223>n是任意核苷
<400>145
atgggaagag caccttgctg tgacaaagcc aacgtcaaaa gaggaccttg gtcacctgaa 60
gaagatgcca aactcaagtc ttacattgaa gaacatggca ccggaggcaa ctggatcgct 120
ctacctcata aaatcggact caaaaggtgt ggcaagagtt gtcgccttag atggttaaat 180
tatcttcgcc caaacataaa gcatggatct ttctccgaag aagaagatca catcatttgt 240
accctatatc ttagtattgg cagcaggtgg tctatcatcg cagcacaatt acctggaaga 300
actgataatg atataaaaaa ctactggaac acgaggctaa agaaaaagct tttgggaaag 360
cagcgtaaag atcaacaatc gtcaaagaga ggtgggcaag tcaagcaaga aatgaagaca 420
gaggtgtttg aggacttaaa ggcaccatct ttatgcatga ccatgaatct ttatcaatca 480
tactggcctt cagaattccc tttgattctc acaaaccctg atcaacatca tcaggaactt 540
cacgtcaaaa accaagatcc caatttcaac ccatacccat ttgacataac ctcaaaccaa 600
gcccaaatgt tccatcagaa ctgtgaaaaa cctatctcta ctacctctta ccatccccag 660
ttggcaacca caccctatta tccaaatggg gatggagtaa acctgtttnc agaatttaaa 720
cattacccat ttgagatcaa tgaattggtt tacaacacca cacaattaga ccagtttgat 780
ggg 783
<210>146
<211>261
<212>PRT
<213>宿根莴苣
<220>
<221>其他特征
<222>(237)..(237)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(247)..(247)
<400>146
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Ser Phe Ser Glu Glu Glu Asp His Ile Ile Cys
65 70 75 80
Thr Leu Tyr Leu Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Asp Gln Gln Ser Ser
115 120 125
Lys Arg Gly Gly Gln Val Lys Gln Glu Met Lys Thr Glu Val Phe Glu
130 135 140
Asp Leu Lys Ala Pro Ser Leu Cys Met Thr Met Asn Leu Tyr Gln Ser
145 150 155 160
Tyr Trp Pro Ser Glu Phe Pro Leu Ile Leu Thr Asn Pro Asp Gln His
165 170 175
His Gln Glu Leu His Val Lys Asn Gln Asp Pro Asn Phe Asn Pro Tyr
180 185 190
Pro Phe Asp Ile Thr Ser Asn Gln Ala Gln Met Phe His Gln Asn Cys
195 200 205
Glu Lys Pro Ile Ser Thr Thr Ser Tyr His Pro Gln Leu Ala Thr Thr
210 215 220
Pro Tyr Tyr Pro Asn Gly Asp Gly Val Asn Leu Phe Xaa Glu Phe Lys
225 230 235 240
His Tyr Pro Phe Glu Ile Asn Glu Leu Val Tyr Asn Thr Thr Gln Leu
245 250 255
Asp Gln Phe Asp Gly
260
<210>147
<211>756
<212>DNA
<213>野生莴苣
<400>147
atgggaaggg ctccatgttg tgacaaggca aacgtgaaga aagggccatg gtcacctgaa 60
gaagatagaa agcttaaaga ctacatagaa acgcatggca ctggtggcaa ctggatcgct 120
ctcccacaaa aagcaggcct tagacgatgt ggaaagagct gcagattgag atggttgaac 180
tatctgagac caaacattaa acatggcgaa ttttctgatg atgaagataa agttatctgt 240
gccctctatg ctagcattgg tagcaggtgg tcaataatgg cagcacagtt accaggaaga 300
acagacaatg atatcaagaa ctactggaac acaaagctga agaagaagat gatgaacagc 360
ttaatcaccc tacccgaact tagaaaacct cttcaacatc ttcaatcttt cagttcttca 420
acaaactaca gctacccatc aaaatcgatt tttcaaaatt gtaatatcaa tgttaatgct 480
ccattatcat catcaatatc atcgtctcca tcatatttgt acagtacaaa tacttctagc 540
taccatgatc aaaccttatc tatcccatct agccctagaa ttaatgctgc taatcgtcaa 600
catccagctc tccaatcaca agatcatgga tttctcggtt tggtttccgc ggagacttac 660
cagcaggggg taaaagacag ttctaccctt gtcttctttg gaggtgatca agctagttgc 720
agttctaatt ccgatgggag ctgccattat gagtat 756
<210>148
<211>252
<212>PRT
<213>野生莴苣
<400>148
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Arg Lys Leu Lys Asp Tyr Ile Glu Thr His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Lys Val Ile Cys
65 70 75 80
Ala Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Met Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Met Met Asn Ser Leu Ile Thr Leu Pro Glu Leu Arg
115 120 125
Lys Pro Leu Gln His Leu Gln Ser Phe Ser Ser Ser Thr Asn Tyr Ser
130 135 140
Tyr Pro Ser Lys Ser Ile Phe Gln Asn Cys Asn Ile Asn Val Asn Ala
145 150 155 160
Pro Leu Ser Ser Ser Ile Ser Ser Ser Pro Ser Tyr Leu Tyr Ser Thr
165 170 175
Asn Thr Ser Ser Tyr His Asp Gln Thr Leu Ser Ile Pro Ser Ser Pro
180 185 190
Arg Ile Asn Ala Ala Asn Arg Gln His Pro Ala Leu Gln Ser Gln Asp
195 200 205
His Gly Phe Leu Gly Leu Val Ser Ala Glu Thr Tyr Gln Gln Gly Val
210 215 220
Lys Asp Ser Ser Thr Leu Val Phe Phe Gly Gly Asp Gln Ala Ser Cys
225 230 235 240
Ser Ser Asn Ser Asp Gly Ser Cys His Tyr Glu Tyr
245 250
<210>149
<211>774
<212>DNA
<213>莴笋
<400>149
atgggaaggg ctccgtgttg tgacaaagaa aatgtaaaga gaggcccatg gtctcctgaa 60
gaagacgcaa aactcaaaag cttcatcgac aaatatggca ctggtggtaa ctggattgct 120
ctccctcaca aagctggtct aaagaggtgt ggaaagagct gcagattgcg atggttaaac 180
tatctgaggc ccaatattaa gcatggtgaa ttcactgatg atgaagacaa gatcatctgc 240
agcttgtatg ctagcattgg tagcagatgg tcaataatag cagctcagct accaggaagg 300
actgataatg atataaagaa ttactggaac accaaactca agaagaagct cttggctatg 360
cttccttcct ttcaaaagaa ggcatctttt tttccatcta tatcccttca atcaccatca 420
ccatacagat catcagatca gttcatgacc gataattctt cccttttcta cggttacact 480
aatttcatga acatgaacaa caatatcaac tctctgctga cacctaccac caacaccacc 540
accggtgttc aagatcatct gatctcacca tcaccaccac caccaccagg agccgatcta 600
aactctttaa tcggtttgat gaccaacaac attgatcaca atgagaattc tttctattta 660
gggtatcagg agaatcatca gagcatgtac aacaccccca tggaatacca ctaccttaca 720
tcagatgtga aagaaccgat gctcattttt ggagtggtgg tgagggtcat gaag 774
<210>150
<211>258
<212>PRT
<213>莴笋
<400>150
Met Gly Arg Ala Pro Cys Cys Asp Lys Glu Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Phe Ile Asp Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Thr Asp Asp Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Ala Met Leu Pro Ser Phe Gln Lys Lys Ala
115 120 125
Ser Phe Phe Pro Ser Ile Ser Leu Gln Ser Pro Ser Pro Tyr Arg Ser
130 135 140
Ser Asp Gln Phe Met Thr Asp Asn Ser Ser Leu Phe Tyr Gly Tyr Thr
145 150 155 160
Asn Phe Met Asn Met Asn Asn Asn Ile Asn Ser Leu Leu Thr Pro Thr
165 170 175
Thr Asn Thr Thr Thr Gly Val Gln Asp His Leu Ile Ser Pro Ser Pro
180 185 190
Pro Pro Pro Pro Gly Ala Asp Leu Asn Ser Leu Ile Gly Leu Met Thr
195 200 205
Asn Asn Ile Asp His Asn Glu Asn Ser Phe Tyr Leu Gly Tyr Gln Glu
210 215 220
Asn His Gln Ser Met Tyr Asn Thr Pro Met Glu Tyr His Tyr Leu Thr
225 230 235 240
Ser Asp Val Lys Glu Pro Met Leu Ile Phe Gly Val Val Val Arg Val
245 250 255
Met Lys
<210>151
<211>816
<212>DNA
<213>莴笋
<400>151
atgggaaggg ctccgtgttg tgacaaagaa aatgtaaaga gaggcccatg gtctcctgaa 60
gaagacgcaa aactcaaaag cttcatcgac aaatatggca ctggtggtaa ctggattgct 120
ctccctcaca aagctggtct aaaaaggtgt ggaaagagct gcagattgcg atggttaaac 180
tatctgaggc ccaatattaa gcatggtgaa ttcactgatg atgaagacaa gatcatctgc 240
agcttgtatg ctagcattgg tagcagatgg tcaataatag cagctcagct accaggaagg 300
actgataatg atataaagaa ttactggaac accaaactca agaagaagct cttggctatg 360
cttccttcct ttcaaaagaa ggcatctttt tttccatcta tatcccttca atcaccatca 420
ccatacagat catcagatca gttcatgacc gataattctt cccttttcta cggttacact 480
aatttcatga acatgaacaa caatatcaac tctctgctga cacctaccac caacaccacc 540
accggtgttc aagatcatct gatctcacca tcaccaccac caccaccagg agccgatcta 600
aactctttaa tcggtttgat gaccaacaac attgacaaca atgagaattc tttctattta 660
gggtatcagg agaatcatca gagcatgtac aacaccccca tggaatacca ctaccttaca 720
tcagatgtga aagaaccgat gctcattttt ggaagtggtg gtgagggtca tgaagtgagc 780
accactggtt cttcctctga agctgggagt tctttg 816
<210>152
<211>272
<212>PRT
<213>莴笋
<400>152
Met Gly Arg Ala Pro Cys Cys Asp Lys Glu Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Phe Ile Asp Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Thr Asp Asp Glu Asp Lys Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Ala Met Leu Pro Ser Phe Gln Lys Lys Ala
115 120 125
Ser Phe Phe Pro Ser Ile Ser Leu Gln Ser Pro Ser Pro Tyr Arg Ser
130 135 140
Ser Asp Gln Phe Met Thr Asp Asn Ser Ser Leu Phe Tyr Gly Tyr Thr
145 150 155 160
Asn Phe Met Asn Met Asn Asn Asn Ile Asn Ser Leu Leu Thr Pro Thr
165 170 175
Thr Asn Thr Thr Thr Gly Val Gln Asp His Leu Ile Ser Pro Ser Pro
180 185 190
Pro Pro Pro Pro Gly Ala Asp Leu Asn Ser Leu Ile Gly Leu Met Thr
195 200 205
Asn Asn Ile Asp Asn Asn Glu Asn Ser Phe Tyr Leu Gly Tyr Gln Glu
210 215 220
Asn His Gln Ser Met Tyr Asn Thr Pro Met Glu Tyr His Tyr Leu Thr
225 230 235 240
Ser Asp Val Lys Glu Pro Met Leu Ile Phe Gly Ser Gly Gly Glu Gly
245 250 255
His Glu Val Ser Thr Thr Gly Ser Ser Ser Glu Ala Gly Ser Ser Leu
260 265 270
<210>153
<211>750
<212>DNA
<213>莴笋
<400>153
atgggaagag ctccatgttg tgacaagtca aaggtgaaga aagggccatg gtcacctcaa 60
gaagatacaa agttgaaaga ctacatacac aaaaatggta ctggaggcaa ctggattgct 120
cttccacata aagcaggcct taaaagatgt gggaaaagct gccgattaag atggttgaac 180
taccttagac cagacatcaa acatggtgaa ttctccgacc atgaagatag actcatctat 240
actctctttt ctagcatcgg tagcaggtgg tcagtaatag cagcacagtt accaggaaga 300
acagataatg atatcaagaa ctactggaac acgaagctca agaagaagat tatgaatttc 360
atctccacca atcatcaaac tgagaaacca cttcatcatc ttgagtttgt caattgtcct 420
tcttcaaact atagctaccc gacaagctct tcggttacaa ctgttgataa tcatccagtt 480
ctaccatcag atgatcatgg atacataaat gtggacatgg aaacctaccg agtaaaagac 540
aattctaccc tttttatgct tgaaggtgat gctcaagctg ctgattgcag ttctaattct 600
gatggaaggt ggcatcatgt gtatggtagt ggtgcgtttg atgcgcgtaa taacatggga 660
ggcttgaaga caagtaattc ctacatggga gttgatggga attattgtga aaaggcaaga 720
gggtgttatg gggattctac catggagttt 750
<210>154
<211>250
<212>PRT
<213>莴笋
<400>154
Met Gly Arg Ala Pro Cys Cys Asp Lys Ser Lys Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Gln Glu Asp Thr Lys Leu Lys Asp Tyr Ile His Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Glu Phe Ser Asp His Glu Asp Arg Leu Ile Tyr
65 70 75 80
Thr Leu Phe Ser Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ile Met Asn Phe Ile Ser Thr Asn His Gln Thr Glu
115 120 125
Lys Pro Leu His His Leu Glu Phe Val Asn Cys Pro Ser Ser Asn Tyr
130 135 140
Ser Tyr Pro Thr Ser Ser Ser Val Thr Thr Val Asp Asn His Pro Val
145 150 155 160
Leu Pro Ser Asp Asp His Gly Tyr Ile Asn Val Asp Met Glu Thr Tyr
165 170 175
Arg Val Lys Asp Asn Ser Thr Leu Phe Met Leu Glu Gly Asp Ala Gln
180 185 190
Ala Ala Asp Cys Ser Ser Asn Ser Asp Gly Arg Trp His His Val Tyr
195 200 205
Gly Ser Gly Ala Phe Asp Ala Arg Asn Asn Met Gly Gly Leu Lys Thr
210 215 220
Ser Asn Ser Tyr Met Gly Val Asp Gly Asn Tyr Cys Glu Lys Ala Arg
225 230 235 240
Gly Cys Tyr Gly Asp Ser Thr Met Glu Phe
245 250
<210>155
<211>893
<212>DNA
<213>毒莴苣
<400>155
ggaagagctc catgttgtga ccagtcaaag gtgaagaaag ggccatggtc acctcaagaa 60
gatacaaagt tgaaagacta catacacaaa aatggtactg gaggcaactg gattgctctt 120
ccacataaag caggccttaa aagatgtggg aaaagctgcc gattaagatg gttgaactac 180
cttagaccag acatcaaaca tggtgaattc tccgaccatg aagatagact catctatact 240
ctcttttcta gcatcggtag caggtggtca gtaatagcag cacagttacc aggaagaaca 300
gataatgata tcaagaacta ctggaacacg aagctcaaga agaagattat gaatttcatc 360
tccaccaatc atcaaactga gaaaccactt catcatcttg agtttgtcaa ttgtccttct 420
tcaaactata gctacccgac aagctcttcg gttacaactg ttgataatca tccagttcta 480
ccatcagatg atcatggata cataaatgtg gacatggaaa cctaccgagt aaaagacaat 540
tctacccttt ttatgcttga aggtgatgct caagctgctg attgcagttc taattctgat 600
ggaaggtggc atcatgtgta tggtagtggt gcgtttgatg cgcgtaataa catgggaggc 660
ttgaagacaa gtaattccta catgggagtt gatgggaatt attgtgaaaa ggcaagaggg 720
tgttatgggg attctactat ggagtttagt cttgaggagt tcaagaagct cattagcact 780
aatctttgca acagtaacac taacaataat ctcaatgtct ttgttgatga actcaaggca 840
gaagagaaca ttatgtacta ctaaactatt ttgatttatt ttggtgtaaa aaa 893
<210>156
<211>702
<212>DNA
<213>毒莴苣
<220>
<221>其他特征
<222>(566)..(566)
<223>n是任意核苷
<400>156
atgggaagag caccttgctg tgacaaagcc aacgtcaaaa gaggaccttg gtcacctgaa 60
gaagatgcca aactcaagtc ttacattgaa gaacatggca ccggaggcaa ctggattgct 120
ctacctcaca aaatcggact tagatggtta aattatcttc gcccaaacat aaagcatgga 180
tctttctccg aagaagaaga tcacatcatt tgcaccccat atcttagtat tggaagccgg 240
tggtctatca tcgcagcaca attacctgga agaactgata atgatataaa aaactactgg 300
aacacgaggc taaagaaaaa gcttttggga aagcagcgta aagatcaaca atcgtcaaag 360
agaggtgcgc aagtcaagca agaaatgaag agagaggtgt ttgaggagtt aaaggcacca 420
tctttatgca tgagcatgaa tctttatcaa tcatactggc cttcagaatt ccctttgatt 480
ctcacaaacc ctgatcaact tcatcaagaa cttcatgtca aaaaccaagt tcccaatttc 540
aaccaatacc cttttgacac aacctncaac caagccgaaa tgttccacca gaactgtgaa 600
aaacctatct ctactaccgc ttatcatccc cagttggcaa acacacacta ttatccaaat 660
ggggatggaa taaacctgtt tcaagaatta acattacccg ta 702
<210>157
<211>234
<212>PRT
<213>毒莴苣
<220>
<221>其他特征
<222>(189)..(189)
<223>X是任意氨基酸
<400>157
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Arg
35 40 45
Trp Leu Asn Tyr Leu Arg Pro Asn Ile Lys His Gly Ser Phe Ser Glu
50 55 60
Glu Glu Asp His Ile Ile Cys Thr Pro Tyr Leu Ser Ile Gly Ser Arg
65 70 75 80
Trp Ser Ile Ile Ala Ala Gln Leu Pro Gly Arg Thr Asp Asn Asp Ile
85 90 95
Lys Asn Tyr Trp Asn Thr Arg Leu Lys Lys Lys Leu Leu Gly Lys Gln
100 105 110
Arg Lys Asp Gln Gln Ser Ser Lys Arg Gly Ala Gln Val Lys Gln Glu
115 120 125
Met Lys Arg Glu Val Phe Glu Glu Leu Lys Ala Pro Ser Leu Cys Met
130 135 140
Ser Met Asn Leu Tyr Gln Ser Tyr Trp Pro Ser Glu Phe Pro Leu Ile
145 150 155 160
Leu Thr Asn Pro Asp Gln Leu His Gln Glu Leu His Val Lys Asn Gln
165 170 175
Val Pro Asn Phe Asn Gln Tyr Pro Phe Asp Thr Thr Xaa Asn Gln Ala
180 185 190
Glu Met Phe His Gln Asn Cys Glu Lys Pro Ile Ser Thr Thr Ala Tyr
195 200 205
His Pro Gln Leu Ala Asn Thr His Tyr Tyr Pro Asn Gly Asp Gly Ile
210 215 220
Asn Leu Phe Gln Glu Leu Thr Leu Pro Val
225 230
<210>158
<211>867
<212>DNA
<213>毒莴苣
<400>158
atgggaagag ctccatgttg tgacaagtca aaggtgaaga aagggccatg gtcacctcaa 60
gaagatacaa agttgaaaga ctacatacac aaaaatggta ccggaggcaa ctggattgct 120
cttccacata aagcaggtct taaaagatgt gggaaaagct gccgattaag atggttgaac 180
tacctaagac cagacatcaa acatggtgaa ttctccgacc atgaagatag actcatctat 240
actctctttt ctagcatcgg tagcaggtgg tcagtaatag cagcacagtt accaggaaga 300
acagataatg atatcaagaa ctactggaac acgaagctca agaagaagat tatgaatttc 360
atctccacca atcatcaaac tgagaaaccg cttcatcatc ttgagtctgt caattgtcct 420
tcttcaaact atagctaccc aacaagctct tcggttacaa ctgttgataa tcatccagtt 480
ctaccatcag atgatcatgg atacataaat gtggacatgg aaacctaccg agtaaaagac 540
aattctaccc tttttatgct tgaaggtgat gctcaagctg ctgatgattg cagttctaat 600
tctgatggaa ggtggcatca tgtgtatggt agtggtgcgt ttgatgctca taataacatg 660
ggaggcttga agacaagtaa ttcctacatg ggagttgatg ggaattattg tgaaaaagca 720
agagggtgtt atggggattc tactatggag tttagtcttg aggagttcaa gaagctcatt 780
agcactaatc tttgcagcag taacactaac aataaatctc atgtctttgt tgatgaaact 840
caggcagaag agaacattat gtactac 867
<210>159
<211>289
<212>PRT
<213>毒莴苣
<400>159
Met Gly Arg Ala Pro Cys Cys Asp Lys Ser Lys Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Gln Glu Asp Thr Lys Leu Lys Asp Tyr Ile His Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Glu Phe Ser Asp His Glu Asp Arg Leu Ile Tyr
65 70 75 80
Thr Leu Phe Ser Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ile Met Asn Phe Ile Ser Thr Asn His Gln Thr Glu
115 120 125
Lys Pro Leu His His Leu Glu Ser Val Asn Cys Pro Ser Ser Asn Tyr
130 135 140
Ser Tyr Pro Thr Ser Ser Ser Val Thr Thr Val Asp Asn His Pro Val
145 150 155 160
Leu Pro Ser Asp Asp His Gly Tyr Ile Asn Val Asp Met Glu Thr Tyr
165 170 175
Arg Val Lys Asp Asn Ser Thr Leu Phe Met Leu Glu Gly Asp Ala Gln
180 185 190
Ala Ala Asp Asp Cys Ser Ser Asn Ser Asp Gly Arg Trp His His Val
195 200 205
Tyr Gly Ser Gly Ala Phe Asp Ala His Asn Asn Met Gly Gly Leu Lys
210 215 220
Thr Ser Asn Ser Tyr Met Gly Val Asp Gly Asn Tyr Cys Glu Lys Ala
225 230 235 240
Arg Gly Cys Tyr Gly Asp Ser Thr Met Glu Phe Ser Leu Glu Glu Phe
245 250 255
Lys Lys Leu Ile Ser Thr Asn Leu Cys Ser Ser Asn Thr Asn Asn Lys
260 265 270
Ser His Val Phe Val Asp Glu Thr Gln Ala Glu Glu Asn Ile Met Tyr
275 280 285
Tyr
<210>160
<211>392
<212>DNA
<213>北美鹅掌揪
<400>160
atggggagag ctccttgctg cgacaaggcc aacgtgaaaa gagggccttg gtctcccgaa 60
gaagacacag ccctcaaaaa ctacgtcgag aaacatggaa gtggtgggaa ttggattgct 120
ttaccccaca aagcaggcct caaacgttgc ggcaagagtt gccgcctgcg gtggcttaat 180
tatcttagac cagacatcaa acatggaggt tttaccgacg aagaagataa catcatatgc 240
gctctctaca aaaacattgg gagcaaatgg tctatcatag cttctcatct gccagggaga 300
acagacaatg atgtgaagaa ctactggaat actaagctga aaaagaagct attggcagga 360
aatacaaatc tcacaaggga tgcttctatt ac 392
<210>161
<211>131
<212>PRT
<213>北美鹅掌揪
<400>161
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Ala Leu Lys Asn Tyr Val Glu Lys His
20 25 30
Gly Ser Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Asp Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ala Leu Tyr Lys Asn Ile Gly Ser Lys Trp Ser Ile Ile Ala Ser His
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Ala Gly Asn Thr Asn Leu Thr Arg Asp Ala
115 120 125
Ser Ile Thr
130
<210>162
<211>563
<212>DNA
<213>苹果
<400>162
atggggagag ctccttgctg tgacaaggca aatgtaaaga aaggaccatg gtcacctgaa 60
gaagatgcaa agctgaaaga gtacatagaa aaatatggga ctggagggaa ttggattgct 120
cttccacaga aagctggtct taggagatgt gggaagagct gcagactaag atggcttaac 180
tatctgaggc ccaacattaa acatggtgaa ttttctgatg aagaagatag aattatctgc 240
aacctcttta ctaacattgg aagcaggtgg tcaataatag cagctcagtt gccaggcagg 300
actgacaatg atatcaaaaa ctactggaac accaagcaaa aaaagaagct catgggcata 360
agcattctcc catcccagct gctaaaatct cacccatctc ttctccttca aacttcatcc 420
acatcctctt cgccgttatc ataccgagga agcaacacca gcactacatt ttacacacaa 480
accaggtctt tcaccggcac tttggagccc atttcttttt cacaaagtct aatgagcagc 540
agttccatta attctgctcc ttc 563
<210>163
<211>188
<212>PRT
<213>苹果
<400>163
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Asn Leu Phe Thr Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Gln Lys Lys Lys Leu Met Gly Ile Ser Ile Leu Pro Ser Gln Leu Leu
115 120 125
Lys Ser His Pro Ser Leu Leu Leu Gln Thr Ser Ser Thr Ser Ser Ser
130 135 140
Pro Leu Ser Tyr Arg Gly Ser Asn Thr Ser Thr Thr Phe Tyr Thr Gln
145 150 155 160
Thr Arg Ser Phe Thr Gly Thr Leu Glu Pro Ile Ser Phe Ser Gln Ser
165 170 175
Leu Met Ser Ser Ser Ser Ile Asn Ser Ala Pro Ser
180 185
<210>164
<211>1044
<212>DNA
<213>苹果
<400>164
atggggaggg ctccttgttg tgacaaagca aacgtgaaga agggaccatg gtcaccagaa 60
gaagattcaa agctaaaaga gtacatagag aagtatggga ctggtgggaa ttggattgct 120
ctcccacaga aagctggtct gaagagatgt gggaaaagct gcagattgag atggcttaac 180
tatctgaggc caaacatcaa acatggagaa ttctctgatg aggaagacag gataatatgc 240
agcctctttg ctagtattgg aagcaggtgg tcagttatag ctgctcagct gccaggcagg 300
actgacaacg atatcaagaa ctactggaac accaagctca agaagaagct catggggatg 360
catatgggtc ctcctcgacc tcacaaccac cataaaaatt tactaaagcc tcccccattt 420
ccttcttcct ctcatcacaa ttaccaaaac caaccattaa ttccatctga acctctatcg 480
tcgctgtaca aagacctaag caattacaac agatctttct tagggtttga agcgccagtg 540
ccattgccac cacaagtttc gctgacgtcc aataatttct caaacatttc caccaactct 600
tctatttttc aaaccctaaa ttacccagct ggagtgaagg aaaataacaa taataatacc 660
ctcctcgtgt ttggaagtga agggagttgc agtacttcgt ctgatggaag ctgtaataat 720
cagatcagct atgactactg cagcagatca gagattaata acatcaacca agaagaaatg 780
ggttttgatc atcagggctt catgatgctt aactatggca accaatggac cgaaaggcca 840
aatgggtttt attttggatc agaaaacaac acattagaat ttactgatct ggacgaagat 900
gttaagcagc agctgattag tactagaagt aaaaataatt ataataataa tactattata 960
aatgagtcct ctaagtctaa cagcttttta ttcaatgtta atgaaagcaa gacagaagat 1020
gatgagaagg tcatgtattt ctac 1044
<210>165
<211>348
<212>PRT
<213>苹果
<400>165
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ser Lys Leu Lys Glu Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Met His Met Gly Pro Pro Arg Pro His
115 120 125
Asn His His Lys Asn Leu Leu Lys Pro Pro Pro Phe Pro Ser Ser Ser
130 135 140
His His Asn Tyr Gln Asn Gln Pro Leu Ile Pro Ser Glu Pro Leu Ser
145 150 155 160
Ser Leu Tyr Lys Asp Leu Ser Asn Tyr Asn Arg Ser Phe Leu Gly Phe
165 170 175
Glu Ala Pro Val Pro Leu Pro Pro Gln Val Ser Leu Thr Ser Asn Asn
180 185 190
Phe Ser Asn Ile Ser Thr Asn Ser Ser Ile Phe Gln Thr Leu Asn Tyr
195 200 205
Pro Ala Gly Val Lys Glu Asn Asn Asn Asn Asn Thr Leu Leu Val Phe
210 215 220
Gly Ser Glu Gly Ser Cys Ser Thr Ser Ser Asp Gly Ser Cys Asn Asn
225 230 235 240
Gln Ile Ser Tyr Asp Tyr Cys Ser Arg Ser Glu Ile Asn Asn Ile Asn
245 250 255
Gln Glu Glu Met Gly Phe Asp His Gln Gly Phe Met Met Leu Asn Tyr
260 265 270
Gly Asn Gln Trp Thr Glu Arg Pro Asn Gly Phe Tyr Phe Gly Ser Glu
275 280 285
Asn Asn Thr Leu Glu Phe Thr Asp Leu Asp Glu Asp Val Lys Gln Gln
290 295 300
Leu Ile Ser Thr Arg Ser Lys Asn Asn Tyr Asn Asn Asn Thr Ile Ile
305 310 315 320
Asn Glu Ser Ser Lys Ser Asn Ser Phe Leu Phe Asn Val Asn Glu Ser
325 330 335
Lys Thr Glu Asp Asp Glu Lys Val Met Tyr Phe Tyr
340 345
<210>166
<211>596
<212>DNA
<213>木薯
<400>166
atgggaaggg ctccttgctg tgataaagct aatgtgaaga aaggcccatg gtcaccagaa 60
gaagatgcaa agcttaaaga ctatatagag aaacaaggga ctgtaggaaa ttggattgct 120
ctccctcaaa aggctggtct caaaagatgt gggaaaagct gcagattaag atggctaaat 180
tatctgagac ccaacatcaa gcatggagat ttttctgatg atgaagataa aataatctgt 240
aaactctatt ccaacattgg gagcaggtgg tcaataatag cagctcagtt gccaggcagg 300
actgataatg atatcaaaaa ctattggaac acaaaactca agaagaagct tatggggatg 360
atgatgattc atccttctca aacaaaatta ccccaccaat taactacaaa gtttgcttct 420
cttctttgtc aggcttcatc atcatcatca tcaataccat catcaccatc tactgcaata 480
tcttcaccat catcttcata tgccctagct aggtctttca ctgaacctat tccattttca 540
agtaacaatt ccttcactgc tgctaataaa tccatcctcc catcccaaga aagctc 596
<210>167
<211>199
<212>PRT
<213>木薯
<400>167
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Lys Gln
20 25 30
Gly Thr Val Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp Asp Glu Asp Lys Ile Ile Cys
65 70 75 80
Lys Leu Tyr Ser Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Met Met Met Ile His Pro Ser Gln Thr
115 120 125
Lys Leu Pro His Gln Leu Thr Thr Lys Phe Ala Ser Leu Leu Cys Gln
130 135 140
Ala Ser Ser Ser Ser Ser Ser Ile Pro Ser Ser Pro Ser Thr Ala Ile
145 150 155 160
Ser Ser Pro Ser Ser Ser Tyr Ala Leu Ala Arg Ser Phe Thr Glu Pro
165 170 175
Ile Pro Phe Ser Ser Asn Asn Ser Phe Thr Ala Ala Asn Lys Ser Ile
180 185 190
Leu Pro Ser Gln Glu Ser Ser
195
<210>168
<211>543
<212>DNA
<213>地钱
<220>
<221>其他特征
<222>(421)..(421)
<223>n是任意核苷
<220>
<221>其他特征
<222>(436)..(436)
<223>n是任意核苷
<220>
<221>其他特征
<222>(478)..(478)
<223>n是任意核苷
<220>
<221>其他特征
<222>(493)..(493)
<223>n是任意核苷
<220>
<221>其他特征
<222>(505)..(505)
<223>n是任意核苷
<220>
<221>其他特征
<222>(509)..(509)
<223>n是任意核苷
<220>
<221>其他特征
<222>(514)..(514)
<223>n是任意核苷
<220>
<221>其他特征
<222>(522)..(522)
<223>n是任意核苷
<220>
<221>其他特征
<222>(523)..(523)
<223>n是任意核苷
<220>
<221>其他特征
<222>(529)..(529)
<223>n是任意核苷
<220>
<221>其他特征
<222>(529)..(529)
<223>n是任意核苷
<220>
<221>其他特征
<222>(541)..(541)
<223>n是任意核苷
<400>168
atgggtcgag ctccatgttg tgacaaggcc aatgtgaaaa agggtccttg gtcgcccgaa 60
gaggatgcca agctcaagtc tttcatcgaa cacaatggca caggtggcaa ttggatcacg 120
ctgccgagca aagcaggtct gaagcgctgc ggaaagagct gcagactccg ctggatcaat 180
tatttgcgtc ccgacatcaa acatggaagc ttcactgaag aagaagaaaa gacaatttat 240
cgcctccacg ctcaaattgg cagcagatgg tccttgatcg ctgctcagct gcctgggaga 300
accgataacg atatcaagaa ttactggaac actcggctga agaagaagct cctggagaga 360
gctataatat ggtgggtgcg gacgtccgca ccactctttc cagatttatc ccatgaatca 420
nccggccaac gatcanatgt caaatgccgt ggggagagaa ggccttctga attctcantt 480
tctgcatgct cangcttatt atcantacnt tcancaacaa cnnccgcant attatcttcc 540
nca 543
<210>169
<211>181
<212>PRT
<213>地钱
<220>
<221>其他特征
<222>(141)..(141)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(146)..(146)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(160)..(160)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(165)..(165)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(169)..(169)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(170)..(170)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(172)..(172)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(175)..(175)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(177)..(177)
<223>X是任意氨基酸
<220>
<221>其他特征
<222>(181)..(181)
<223>X是任意氨基酸
<400>169
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Phe Ile Glu His Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Thr Leu Pro Ser Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Ser Phe Thr Glu Glu Glu Glu Lys Thr Ile Tyr
65 70 75 80
Arg Leu His Ala Gln Ile Gly Ser Arg Trp Ser Leu Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Glu Arg Ala Ile Ile Trp Trp Val Arg Thr
115 120 125
Ser Ala Pro Leu Phe Pro Asp Leu Ser His Glu Ser Xaa Gly Gln Arg
130 135 140
Ser Xaa Val Lys Cys Arg Gly Glu Arg Arg Pro Ser Glu Phe Ser Xaa
145 150 155 160
Ser Ala Cys Ser Xaa Leu Leu Ser Xaa Xaa Ser Xaa Thr Thr Xaa Ala
165 170 175
Xaa Leu Ser Ser Xaa
180
<210>170
<211>1050
<212>DNA
<213>截形苜蓿
<400>170
atgggtagag ctccatgctg tgacaaggct aacgtgaaga aaggaccttg gtctcctgaa 60
gaagatgcta cactcaaatc ttacattgaa acaaatggaa ctggaggaaa ttggattgct 120
cttcctcaaa aaattgggct caagagatgt ggaaagagtt gcagacttag gtggttaaat 180
tacttgagac ctaatatcaa acatggtgga tttactgaag aagaagacaa catcatttgc 240
agcctttaca taagcattgg aagcaggtgg tccattattg ctgctcagtt acctggaaga 300
acagacaatg acataaaaaa ctattggaac acaagattga agaagaaatt actcggaaag 360
cgaaaacagt cgaatataaa caactgcttg atgaaccaaa aggacacaaa tggaatagat 420
gagaattctt attcaaattc attaagtagc tcagctcttg agagacttca acttcatatg 480
caacttcaaa gccttcaaaa ccctttgtct ttttacaata ataaccctgc tgcactagtt 540
tggccaaagt tgcatccttc tcaagaaaaa atgatccaaa ttagccttca aaactctaat 600
aacaacccta tgatgcaaaa tgctttctct tcaccacagg ttgatctttt ggagaatatt 660
attcctttgg agaataataa taacaattca gttaccttca atgcttctgg aaatagtagt 720
aataataata attcaatcat gcattcaagt gttgcaccaa gaggagaagc tgttgagaag 780
agtactaaca atgaaggaat tcaggaactg gaaagtgaac tagatgaaat tctcaacaac 840
agaaatataa ttactatgga agatgaatat cgtgtggctg aatttgattg tttcagagat 900
atgaataata atggttcaaa ggatcaaaac ttgatatggt ggtcaaatga ttcaggtgat 960
actaaatcag gatcctcaaa ctcatgggat tcaacaacta atcttatgca agaagggatg 1020
ttccaagatt atgaactagg ttatggtctg 1050
<210>171
<211>350
<212>PRT
<213>截形苜蓿
<400>171
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Thr Leu Lys Ser Tyr Ile Glu Thr Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Arg Lys Gln Ser Asn Ile Asn Asn
115 120 125
Cys Leu Met Asn Gln Lys Asp Thr Asn Gly Ile Asp Glu Asn Ser Tyr
130 135 140
Ser Asn Ser Leu Ser Ser Ser Ala Leu Glu Arg Leu Gln Leu His Met
145 150 155 160
Gln Leu Gln Ser Leu Gln Asn Pro Leu Ser Phe Tyr Asn Asn Asn Pro
165 170 175
Ala Ala Leu Val Trp Pro Lys Leu His Pro Ser Gln Glu Lys Met Ile
180 185 190
Gln Ile Ser Leu Gln Asn Ser Asn Asn Asn Pro Met Met Gln Asn Ala
195 200 205
Phe Ser Ser Pro Gln Val Asp Leu Leu Glu Asn Ile Ile Pro Leu Glu
210 215 220
Asn Asn Asn Asn Asn Ser Val Thr Phe Asn Ala Ser Gly Asn Ser Ser
225 230 235 240
Asn Asn Asn Asn Ser Ile Met His Ser Ser Val Ala Pro Arg Gly Glu
245 250 255
Ala Val Glu Lys Ser Thr Asn Asn Glu Gly Ile Gln Glu Leu Glu Ser
260 265 270
Glu Leu Asp Glu Ile Leu Asn Asn Arg Asn Ile Ile Thr Met Glu Asp
275 280 285
Glu Tyr Arg Val Ala Glu Phe Asp Cys Phe Arg Asp Met Asn Asn Asn
290 295 300
Gly Ser Lys Asp Gln Asn Leu Ile Trp Trp Ser Asn Asp Ser Gly Asp
305 310 315 320
Thr Lys Ser Gly Ser Ser Asn Ser Trp Asp Ser Thr Thr Asn Leu Met
325 330 335
Gln Glu Gly Met Phe Gln Asp Tyr Glu Leu Gly Tyr Gly Leu
340 345 350
<210>172
<211>576
<212>DNA
<213>截形苜蓿
<400>172
atgggtagag ctccttgttg tgacaaagca aacgtgaaga aaggtccatg gtctccagaa 60
gaagattcta aactcaaatc ttacatagaa caacatggta ctggtggtaa ctggattgct 120
ctcccacaaa aaattggctt gaagcgttgt ggaaagagct gtcgtctccg gtggctaaac 180
tatcttcgcc ctaatctcaa acatggtggt ttctccgaag aagaagataa cattatttgc 240
agcctttaca ttagtattgg aagcaggtgg tcgataattg cagctcaatt gccaggaaga 300
actgataatg acataaagaa ctattggaat acaaggttga aaaagaagct tttggggaaa 360
caccgtaaag atcaacaaca acaagcacgt aatagaggaa ataatggtgc tattgttaag 420
caagaaagca ataataatag agtgatgagt agtaatgaat tttccttatc aaatttggtt 480
caagaacaac catatttacc acatgtcatg caacctttgc tatcaacacc ccaacctcca 540
ccaccatcaa tgttgtcata cacaaaccaa caaggc 576
<210>173
<211>192
<212>PRT
<213>截形苜蓿
<400>173
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ser Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Leu Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys His Arg Lys Asp Gln Gln Gln Gln
115 120 125
Ala Arg Asn Arg Gly Asn Asn Gly Ala Ile Val Lys Gln Glu Ser Asn
130 135 140
Asn Asn Arg Val Met Ser Ser Asn Glu Phe Ser Leu Ser Asn Leu Val
145 150 155 160
Gln Glu Gln Pro Tyr Leu Pro His Val Met Gln Pro Leu Leu Ser Thr
165 170 175
Pro Gln Pro Pro Pro Pro Ser Met Leu Ser Tyr Thr Asn Gln Gln Gly
180 185 190
<210>174
<211>596
<212>DNA
<213>睡莲科萍蓬草属
<400>174
atgggaaggt ctccgtgctg tgacaaggcc aacgttaaga gagggccatg gtcgcctgag 60
gaggacgcca ccctcaagaa ctacgttgag aggtttggca ccggaggcaa ttggattgca 120
ttgcctcaga aagcagggct caagcgttgt ggaaagagct gccgtttgcg ttggcttaac 180
taccttagac ctgatattag gcacggtggt tttactgagg aagaggatac catcattttc 240
tctctctatg agagcatggg cagcaggtgg tctgtcatag catcacagtt accaggaaga 300
accgacaatg atgtgaagaa ttactggaac accaagttga agaagaaaat gcttgcagca 360
aaagccaatc ttgacagtga tgcccatatt cacatgaatt cagtttcaat ctcatcatca 420
tcttcacctt caccttcacc ttcctcttca tcaccatcca cttccactcc tacccattta 480
accattgcat caaaaagtgt gggccctgcc tattacttct ccaaactcag ctgcaccaaa 540
tcccaagatg aaggcatggt gatgcctcca atggaagttg gctttcagta cactct 596
<210>175
<211>199
<212>PRT
<213>睡莲科萍蓬草属
<400>175
Met Gly Arg Ser Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Thr Leu Lys Asn Tyr Val Glu Arg Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Glu Glu Glu Asp Thr Ile Ile Phe
65 70 75 80
Ser Leu Tyr Glu Ser Met Gly Ser Arg Trp Ser Val Ile Ala Ser Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Met Leu Ala Ala Lys Ala Asn Leu Asp Ser Asp Ala
115 120 125
His Ile His Met Asn Ser Val Ser Ile Ser Ser Ser Ser Ser Pro Ser
130 135 140
Pro Ser Pro Ser Ser Ser Ser Pro Ser Thr Ser Thr Pro Thr His Leu
145 150 155 160
Thr Ile Ala Ser Lys Ser Val Gly Pro Ala Tyr Tyr Phe Ser Lys Leu
165 170 175
Ser Cys Thr Lys Ser Gln Asp Glu Gly Met Val Met Pro Pro Met Glu
180 185 190
Val Gly Phe Gln Tyr Thr Leu
195
<210>176
<211>795
<212>DNA
<213>水稻
<400>176
atggggagga cgccgtgctg cgacagggag gcggtgaaga ggggcccgtg gtcgccggag 60
gaggacgacg cgctgcgcga ctacatcaac cgccacggca ccgccggcaa ctggatctcc 120
ctccccaaca aggccgggtt gaggaggtgc ggcaagagct gcaggctgcg gtggctcaac 180
tacctccgcc ccgacatccg ccatggcgcc ttcaccgacg aggaggacgc catcatcacc 240
tccctctact ccaagctcgg cagcaagtgg tcgaccatcg cggcgcagct ggagaggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agcgccgcct cgccgccgcc 360
gccgcctgca cgcccttact gccgctcccg gcgccgccgc ccctcgccgc cacgcacacg 420
tcgccgtcgt cgtcgctgct gctcctcccg ccgctcgccg taccgaccgt caagaccgag 480
gcgtacacct gcgacgactt cctgcagcag ctgctgccga ccgccaccgc cgccacggcg 540
ctccgggatc ccttcgccga cggcgccgcc acggacggcg gctcgacgtc cgcctccgcc 600
gcgtcgtcgg ggtccaactg gtcggcggac accggcgtcg tcgtcgtcgg tggcggcggc 660
ggcggcgggc tgttcccgga attctgcatg agctccgacg acctcgccgg cgccgccacg 720
gcggaggacg accacttcat cggcggcggc tactactacc ctctcgatcc gagcttgtca 780
tcatcactag tgtag 795
<210>177
<211>264
<212>PRT
<213>水稻
<400>177
Met Gly Arg Thr Pro Cys Cys Asp Arg Glu Ala Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Asp Ala Leu Arg Asp Tyr Ile Asn Arg His
20 25 30
Gly Thr Ala Gly Asn Trp Ile Ser Leu Pro Asn Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Ala Phe Thr Asp Glu Glu Asp Ala Ile Ile Thr
65 70 75 80
Ser Leu Tyr Ser Lys Leu Gly Ser Lys Trp Ser Thr Ile Ala Ala Gln
85 90 95
Leu Glu Arg Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Arg Arg Leu Ala Ala Ala Ala Ala Cys Thr Pro Leu Leu Pro
115 120 125
Leu Pro Ala Pro Pro Pro Leu Ala Ala Thr His Thr Ser Pro Ser Ser
130 135 140
Ser Leu Leu Leu Leu Pro Pro Leu Ala Val Pro Thr Val Lys Thr Glu
145 150 155 160
Ala Tyr Thr Cys Asp Asp Phe Leu Gln Gln Leu Leu Pro Thr Ala Thr
165 170 175
Ala Ala Thr Ala Leu Arg Asp Pro Phe Ala Asp Gly Ala Ala Thr Asp
180 185 190
Gly Gly Ser Thr Ser Ala Ser Ala Ala Ser Ser Gly Ser Asn Trp Ser
195 200 205
Ala Asp Thr Gly Val Val Val Val Gly Gly Gly Gly Gly Gly Gly Leu
210 215 220
Phe Pro Glu Phe Cys Met Ser Ser Asp Asp Leu Ala Gly Ala Ala Thr
225 230 235 240
Ala Glu Asp Asp His Phe Ile Gly Gly Gly Tyr Tyr Tyr Pro Leu Asp
245 250 255
Pro Ser Leu Ser Ser Ser Leu Val
260
<210>178
<211>957
<212>DNA
<213>水稻
<400>178
atggggaggg cgccgtgctg cgacaaggcg agcgtgaaga gggggccgtg gtcgccggag 60
gaggacgagc tgctgcggag ctacgtccgc agccacggca ccggtggcaa ctggatcgcg 120
ctcccgcaga aagcagggct gaaccggtgc gggaagagct gtaggctgcg gtggctcaac 180
tacctccgcc cggacatcaa gcacggcggc tacaccgacc aggaggaccg gatcatctgt 240
tccctctaca actccatcgg aagcaggtgg tccatcatcg cgtcgaagct gcccggccgg 300
acggacaacg acgtcaagaa ttactggaat accaagctca agaagaaggc catggccatg 360
catcatcatc atcagccgcc gccgccgcag cagcaacact accaccacca ccaccaccac 420
cgtgtcgccg gcggtggcgc gcgcgtcacg ctcgtgtcgc ctccgcccgc cccgcagagc 480
caatgcgcgt ccatgcagcc gtcgccggcg tccgcctcct cgtccggcgg cgacgcgtgc 540
agcttcggcg ccgccgccat gtactccccc tccccgtcaa cccagcaggc gccacaggcg 600
gcgacgctcg cggtcgcggg gtacacctcc gtggcgacgg cggcggcggc ggcggcggtg 660
gcggcgcagc gctcgccgct cgacgagctg atctgccagg tgccaccacc tcccactact 720
accgccgccg actgctgggc cagcggcgtg accctcgacg acgtgttctt gcccgagctc 780
gtcggagccg gcgagttccc caacggcgac ctcttcggcg ggttcggccc gctgctccag 840
gacaggtcgt ccatggagct ctccgcgtgc tacttcccca acgccgcggc ggcggagatg 900
tggccggcgg ccacggacat cgtcaagccg gccgggctgt gccacagcct gacatga 957
<210>179
<211>318
<212>PRT
<213>水稻
<400>179
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Leu Leu Arg Ser Tyr Val Arg Ser His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Tyr Thr Asp Gln Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Asn Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ala Met Ala Met His His His His Gln Pro Pro Pro
115 120 125
Pro Gln Gln Gln His Tyr His His His His His His Arg Val Ala Gly
130 135 140
Gly Gly Ala Arg Val Thr Leu Val Ser Pro Pro Pro Ala Pro Gln Ser
145 150 155 160
Gln Cys Ala Ser Met Gln Pro Ser Pro Ala Ser Ala Ser Ser Ser Gly
165 170 175
Gly Asp Ala Cys Ser Phe Gly Ala Ala Ala Met Tyr Ser Pro Ser Pro
180 185 190
Ser Thr Gln Gln Ala Pro Gln Ala Ala Thr Leu Ala Val Ala Gly Tyr
195 200 205
Thr Ser Val Ala Thr Ala Ala Ala Ala Ala Ala Val Ala Ala Gln Arg
210 215 220
Ser Pro Leu Asp Glu Leu Ile Cys Gln Val Pro Pro Pro Pro Thr Thr
225 230 235 240
Thr Ala Ala Asp Cys Trp Ala Ser Gly Val Thr Leu Asp Asp Val Phe
245 250 255
Leu Pro Glu Leu Val Gly Ala Gly Glu Phe Pro Asn Gly Asp Leu Phe
260 265 270
Gly Gly Phe Gly Pro Leu Leu Gln Asp Arg Ser Ser Met Glu Leu Ser
275 280 285
Ala Cys Tyr Phe Pro Asn Ala Ala Ala Ala Glu Met Trp Pro Ala Ala
290 295 300
Thr Asp Ile Val Lys Pro Ala Gly Leu Cys His Ser Leu Thr
305 310 315
<210>180
<211>897
<212>DNA
<213>水稻
<400>180
atggggaggt cgccgtgctg cgacaaggcg agcgtgaagc gggggccgtg gtcggaggag 60
gaggacgcca tactcaggag cttcgtcgag aggttcggca atgccggcaa ctggatcgcg 120
ctgccccaca aagcagggct taaacggtgc ggcaagagct gccgcctccg gtggctcaac 180
tacctccgcc cggcgatccg gcacggcggc ttcaccgacg aggaggacaa cctcatcctg 240
tcgctctacg gcgaaatggg aagcaagtgg tcggtgatcg cgtccaagct ccccggccgg 300
acggacaacg acgtcaagaa ctactggaac accaagctca agaagaggta cttggccgcg 360
gccgcaacag aagcaaccac tcctcctcct cctgccgccg gcgacgatga caacaaccca 420
acgacacaag cctccagcca acccgctcct cctactcctc cggcgcccct cgtcaacctc 480
gacgcggccg gcctcgacgg cgccgtgggc gacaacgacg agctcctgct gcacaagtcg 540
gagcagctgt acgccgagct gatgggcctc atcgagcagc agcagtactc cacgatcacc 600
gccgccgccg tcgacgcggc gacgacgacg acgtcgtggt cgtcgccgtc gacgggaacg 660
acaagtccga ccgctagcag cagtactgac ggcagcagca gcagcagcaa cctgccgtgg 720
ccggccgtgg acgtgcacga cagtacgatg atgccgccgt tgtcggagtc cagcggcagc 780
agcagcggct tgttcttcgg ctctcacgcg ttcggtagtg gctcgttcca agacctgctc 840
ggctctgcag cttccttcga cgatgtcatg ctgtcgcaag agatgctgta ctactag 897
<210>181
<211>298
<212>PRT
<213>水稻
<400>181
Met Gly Arg Ser Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Glu Glu Glu Asp Ala Ile Leu Arg Ser Phe Val Glu Arg Phe
20 25 30
Gly Asn Ala Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Ala Ile Arg His Gly Gly Phe Thr Asp Glu Glu Asp Asn Leu Ile Leu
65 70 75 80
Ser Leu Tyr Gly Glu Met Gly Ser Lys Trp Ser Val Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Tyr Leu Ala Ala Ala Ala Thr Glu Ala Thr Thr Pro
115 120 125
Pro Pro Pro Ala Ala Gly Asp Asp Asp Asn Asn Pro Thr Thr Gln Ala
130 135 140
Ser Ser Gln Pro Ala Pro Pro Thr Pro Pro Ala Pro Leu Val Asn Leu
145 150 155 160
Asp Ala Ala Gly Leu Asp Gly Ala Val Gly Asp Asn Asp Glu Leu Leu
165 170 175
Leu His Lys Ser Glu Gln Leu Tyr Ala Glu Leu Met Gly Leu Ile Glu
180 185 190
Gln Gln Gln Tyr Ser Thr Ile Thr Ala Ala Ala Val Asp Ala Ala Thr
195 200 205
Thr Thr Thr Ser Trp Ser Ser Pro Ser Thr Gly Thr Thr Ser Pro Thr
210 215 220
Ala Ser Ser Ser Thr Asp Gly Ser Ser Ser Ser Ser Asn Leu Pro Trp
225 230 235 240
Pro Ala Val Asp Val His Asp Ser Thr Met Met Pro Pro Leu Ser Glu
245 250 255
Ser Ser Gly Ser Ser Ser Gly Leu Phe Phe Gly Ser His Ala Phe Gly
260 265 270
Ser Gly Ser Phe Gln Asp Leu Leu Gly Ser Ala Ala Ser Phe Asp Asp
275 280 285
Val Met Leu Ser Gln Glu Met Leu Tyr Tyr
290 295
<210>182
<211>1692
<212>DNA
<213>水稻
<400>182
atggcggcgg cggcggctcg cgatgacgac ctcggcaacg acgacctcga cgatgaccgg 60
tcaaatccac gccgccagtc tggaggtcgc acggccggcc caatccggca ggggttgcat 120
ggtgacatag gcctccaaat gacggcagcc atggctggtg acgacgcaaa tgacggcggc 180
catggctggc ggcggtggct gtcaacggcg gcggtcgcgg cggtgctcct cagcttaggg 240
gatggtggtg acgatgacaa ggcaaccgag gggatacggg ggagctcagc agggatgtat 300
actgataaaa gatcagtttc agaggacaca cacctctatc catctactaa acctactata 360
aagccagccc ggccacaacc ccagatgcac ccgctcaccg ctgctgccaa acacgccatc 420
accataccac caccaccacc accagccgca gcagctagct acacatcgtc gccgtcgtca 480
ggcactagcg atccatcggt tctagatctc tcttctgctg agatagacga cgacggcgac 540
ggcgacgacg atcgagctga gcagcaagaa atcaagaatt cgaaggagtt agtgatgggg 600
agggcgccgt gctgcgacaa ggcgagcgtg aagaaagggc cgtggtcgcc ggaggaggac 660
gccaagctca aggcctacat cgaggagaac ggcaccggcg gcaactggat cgcgctgccg 720
cagaagatcg ggctgaagag atgtggcaag agttgcaggc tcagatggct caactacctg 780
cggccaaaca ttaagcatgg tgatttcaca gaagaagagg agcacatcat ttgtagcctc 840
tacattagca tcggtagcag gtggtcgatc atcgcggcgc agctgccggg gagaacggac 900
aacgacatca agaactactg gaacaccaag ctgaagaaga agctcctcgg caagcgcgcg 960
ccgtcgcgcc gcgcccgcgc aaaccaagac cactgcggtc tagcaggcag cgccgccgcc 1020
gccatgtgcg gtggcgtcgg caccgcggcg gcggcggcgc cgccgcatca agccctaagc 1080
tcgtcggccc tcgagcggat ccagctccac atgcgcctcc aaggcctcta caacagcgcg 1140
ttcggctgca ccaccaccag cagcaacggc ggcggcgtcg gcgtcgcgcc gccgcagtgg 1200
ccgaagctcg aggcgctgct gccgagcaga ccgctcccgg ccgtgcagcc gacggacgcc 1260
gtcgtcgcca ccgtgcaaca cccccatcat ttggtcgtcg gcggccatac cctcgccacc 1320
gccgccgccg ccgccgccac cacgtcggag gcgttccaag ccgccgagca cctcgaccct 1380
gcggcggcga ccggttcgaa ctacatgccg ggagtcgccg gtgtagagat gacctcgtcg 1440
tcgtcaatgg cgggtggtgg tgggttcgtc gccggctacg gtctccacga cgagctatac 1500
gacttcctct tcaagtgcga gtcgatcggc ggagcgcaag gcgggatcat cccttcgtcg 1560
ttgccggagc tgcagtgccc ggacggcagc gccatcatcg gcgccgacga gaagttctcg 1620
acgtggacgt cgtcgtcttg cgactacggt tcgggcggcg ccggcgatta cgttctaggg 1680
tatgatcaat aa 1692
<210>183
<211>563
<212>PRT
<213>水稻
<400>183
Met Ala Ala Ala Ala Ala Arg Asp Asp Asp Leu Gly Asn Asp Asp Leu
1 5 10 15
Asp Asp Asp Arg Ser Asn Pro Arg Arg Gln Ser Gly Gly Arg Thr Ala
20 25 30
Gly Pro Ile Arg Gln Gly Leu His Gly Asp Ile Gly Leu Gln Met Thr
35 40 45
Ala Ala Met Ala Gly Asp Asp Ala Asn Asp Gly Gly His Gly Trp Arg
50 55 60
Arg Trp Leu Ser Thr Ala Ala Val Ala Ala Val Leu Leu Ser Leu Gly
65 70 75 80
Asp Gly Gly Asp Asp Asp Lys Ala Thr Glu Gly Ile Arg Gly Ser Ser
85 90 95
Ala Gly Met Tyr Thr Asp Lys Arg Ser Val Ser Glu Asp Thr His Leu
100 105 110
Tyr Pro Ser Thr Lys Pro Thr Ile Lys Pro Ala Arg Pro Gln Pro Gln
115 120 125
Met His Pro Leu Thr Ala Ala Ala Lys His Ala Ile Thr Ile Pro Pro
130 135 140
Pro Pro Pro Pro Ala Ala Ala Ala Ser Tyr Thr Ser Ser Pro Ser Ser
145 150 155 160
Gly Thr Ser Asp Pro Ser Val Leu Asp Leu Ser Ser Ala Glu Ile Asp
165 170 175
Asp Asp Gly Asp Gly Asp Asp Asp Arg Ala Glu Gln Gln Glu Ile Lys
180 185 190
Asn Ser Lys Glu Leu Val Met Gly Arg Ala Pro Cys Cys Asp Lys Ala
195 200 205
Ser Val Lys Lys Gly Pro Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys
210 215 220
Ala Tyr Ile Glu Glu Asn Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro
225 230 235 240
Gln Lys Ile Gly Leu Lys Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp
245 250 255
Leu Asn Tyr Leu Arg Pro Asn Ile Lys His Gly Asp Phe Thr Glu Glu
260 265 270
Glu Glu His Ile Ile Cys Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp
275 280 285
Ser Ile Ile Ala Ala Gln Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys
290 295 300
Asn Tyr Trp Asn Thr Lys Leu Lys Lys Lys Leu Leu Gly Lys Arg Ala
305 310 315 320
Pro Ser Arg Arg Ala Arg Ala Asn Gln Asp His Cys Gly Leu Ala Gly
325 330 335
Ser Ala Ala Ala Ala Met Cys Gly Gly Val Gly Thr Ala Ala Ala Ala
340 345 350
Ala Pro Pro His Gln Ala Leu Ser Ser Ser Ala Leu Glu Arg Ile Gln
355 360 365
Leu His Met Arg Leu Gln Gly Leu Tyr Asn Ser Ala Phe Gly Cys Thr
370 375 380
Thr Thr Ser Ser Asn Gly Gly Gly Val Gly Val Ala Pro Pro Gln Trp
385 390 395 400
Pro Lys Leu Glu Ala Leu Leu Pro Ser Arg Pro Leu Pro Ala Val Gln
405 410 415
Pro Thr Asp Ala Val Val Ala Thr Val Gln His Pro His His Leu Val
420 425 430
Val Gly Gly His Thr Leu Ala Thr Ala Ala Ala Ala Ala Ala Thr Thr
435 440 445
Ser Glu Ala Phe Gln Ala Ala Glu His Leu Asp Pro Ala Ala Ala Thr
450 455 460
Gly Ser Asn Tyr Met Pro Gly Val Ala Gly Val Glu Met Thr Ser Ser
465 470 475 480
Ser Ser Met Ala Gly Gly Gly Gly Phe Val Ala Gly Tyr Gly Leu His
485 490 495
Asp Glu Leu Tyr Asp Phe Leu Phe Lys Cys Glu Ser Ile Gly Gly Ala
500 505 510
Gln Gly Gly Ile Ile Pro Ser Ser Leu Pro Glu Leu Gln Cys Pro Asp
515 520 525
Gly Ser Ala Ile Ile Gly Ala Asp Glu Lys Phe Ser Thr Trp Thr Ser
530 535 540
Ser Ser Cys Asp Tyr Gly Ser Gly Gly Ala Gly Asp Tyr Val Leu Gly
545 550 555 560
Tyr Asp Gln
<210>184
<211>870
<212>DNA
<213>水稻
<400>184
atggggcgcg cgccgtgctg cgacaaggcg agcgtgaagc gggggccgtg gtcgccggag 60
gaggacgagc agctgcggag ctacgtccag agccacggca tcggcggcaa ctggatcgcg 120
ctcccgcaga aagcagggct caaccgctgc ggcaagagct gcaggctccg gtggctcaac 180
tacctgaggc cggacatcaa gcacggcggc tacaccgagc aggaggacca catcatctgc 240
tcgctctaca actcgattgg aagcaggtgg tccatcatcg cgtcgaagct ccccggccgg 300
actgacaacg acgtcaagaa ctactggaac accaagctca agaagaaagc catgggcgcc 360
gtgcagccgc gcgccgccgc ctcggcgccg agccaatgca cgtcgtcagc gatggcgccg 420
gcgctctcgc cggcctcctc gtccgtcacc agctcgagcg gcgacgcctg cttcgccgcc 480
gccgccacca ccaccaccac catgtacccg ccaccgacga cgccgccgca gcagcagttc 540
atccgcttcg acgcaccacc cgcggcggcg gcggcggcat ccccgaccga cctcgcgccc 600
gtgccaccac cggccaccgt cacggcggac ggcgacggcg gctgggcgtc cgacgccttg 660
tccctcgacg acgtgttcct cggcgagctc acggccggcg agccgctgtt cccttacgcc 720
gagctgttca gcggcttcgc cggcgcggcg ccggacagca aggccacctt ggagctctcg 780
gcgtgctact tcccgaacat ggcggagatg tgggcggcct ccgaccacgc ctacgccaag 840
ccacagggtc tctgcaacac cctgacatag 870
<210>185
<211>289
<212>PRT
<213>水稻
<400>185
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Gln Leu Arg Ser Tyr Val Gln Ser His
20 25 30
Gly Ile Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Tyr Thr Glu Gln Glu Asp His Ile Ile Cys
65 70 75 80
Ser Leu Tyr Asn Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ala Met Gly Ala Val Gln Pro Arg Ala Ala Ala Ser
115 120 125
Ala Pro Ser Gln Cys Thr Ser Ser Ala Met Ala Pro Ala Leu Ser Pro
130 135 140
Ala Ser Ser Ser Val Thr Ser Ser Ser Gly Asp Ala Cys Phe Ala Ala
145 150 155 160
Ala Ala Thr Thr Thr Thr Thr Met Tyr Pro Pro Pro Thr Thr Pro Pro
165 170 175
Gln Gln Gln Phe Ile Arg Phe Asp Ala Pro Pro Ala Ala Ala Ala Ala
180 185 190
Ala Ser Pro Thr Asp Leu Ala Pro Val Pro Pro Pro Ala Thr Val Thr
195 200 205
Ala Asp Gly Asp Gly Gly Trp Ala Ser Asp Ala Leu Ser Leu Asp Asp
210 215 220
Val Phe Leu Gly Glu Leu Thr Ala Gly Glu Pro Leu Phe Pro Tyr Ala
225 230 235 240
Glu Leu Phe Ser Gly Phe Ala Gly Ala Ala Pro Asp Ser Lys Ala Thr
245 250 255
Leu Glu Leu Ser Ala Cys Tyr Phe Pro Asn Met Ala Glu Met Trp Ala
260 265 270
Ala Ser Asp His Ala Tyr Ala Lys Pro Gln Gly Leu Cys Asn Thr Leu
275 280 285
Thr
<210>186
<211>951
<212>DNA
<213>水稻
<400>186
atggggaggg caccttgctg tgacaaggca acagtgaaga aggggccatg gtcacctgag 60
gaggatgcaa tgctcaagaa ctacattgag gagcatggca ccggtggcaa ctggattgca 120
ctgcctcaca agattgggct gaagaggtgt ggcaagagct gcaggctaag gtggctgaat 180
tacctgaggc caaacataaa gcatggggac ttcaccccag aggaggacag catcatctgc 240
agcctctaca ttagcatagg gagcaggtgg tcaatcatag cagcacagct gccagggagg 300
acggacaacg atgtcaagaa ctactggaac acaaagctga agaagagact ccttggccgg 360
cgcaaggacc gcggcggcgg ccaccaccac cgcagccaga gcaccgccga cgatcttccg 420
gccggtggtg acggcggcat gaacgacggc ggcggcggcg gcggagagcg gtcgctgagc 480
gcgtcggcga tggagaggat ccagctctgc atgcagctgc aggagctgca gaacccactg 540
tccatccacc acaacccctt gctctctcat cagtggccaa gcaaggccac cattgatgat 600
cagaatcaca acaatgtcac tgtggctgaa catggaatgt caagctctgt gagcgaccac 660
caccgcctcg atgggcagca gctggagagc ggcgccggcg ccgccgccat gcagcaggcg 720
tcgccgtcga gcggcggcga gaactccaac gtcgtcgtcg ccatcgaggc cgagctccag 780
gagcttctct acgccggcgg cggcgcgatc gtcgacggcg gcgcgccgcc gcagggggat 840
gtggactggt ggagctatga tcagggaaag cagtcacctg tgacttgctg ggatttcacc 900
cctgaaacca gctccatctt ccaggattat gcaacagttt atgacatctg a 951
<210>187
<211>316
<212>PRT
<213>水稻
<400>187
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Met Leu Lys Asn Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Thr Pro Glu Glu Asp Ser Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Leu Gly Arg Arg Lys Asp Arg Gly Gly Gly His
115 120 125
His His Arg Ser Gln Ser Thr Ala Asp Asp Leu Pro Ala Gly Gly Asp
130 135 140
Gly Gly Met Asn Asp Gly Gly Gly Gly Gly Gly Glu Arg Ser Leu Ser
145 150 155 160
Ala Ser Ala Met Glu Arg Ile Gln Leu Cys Met Gln Leu Gln Glu Leu
165 170 175
Gln Asn Pro Leu Ser Ile His His Asn Pro Leu Leu Ser His Gln Trp
180 185 190
Pro Ser Lys Ala Thr Ile Asp Asp Gln Asn His Asn Asn Val Thr Val
195 200 205
Ala Glu His Gly Met Ser Ser Ser Val Ser Asp His His Arg Leu Asp
210 215 220
Gly Gln Gln Leu Glu Ser Gly Ala Gly Ala Ala Ala Met Gln Gln Ala
225 230 235 240
Ser Pro Ser Ser Gly Gly Glu Asn Ser Asn Val Val Val Ala Ile Glu
245 250 255
Ala Glu Leu Gln Glu Leu Leu Tyr Ala Gly Gly Gly Ala Ile Val Asp
260 265 270
Gly Gly Ala Pro Pro Gln Gly Asp Val Asp Trp Trp Ser Tyr Asp Gln
275 280 285
Gly Lys Gln Ser Pro Val Thr Cys Trp Asp Phe Thr Pro Glu Thr Ser
290 295 300
Ser Ile Phe Gln Asp Tyr Ala Thr Val Tyr Asp Ile
305 310 315
<210>188
<211>1134
<212>DNA
<213>水稻
<400>188
atgggcaggg cgccgtgctg cgacaaggcg acggtgaaga agggcccgtg ggcgccggag 60
gaggacgccg cgctcaaggc ctacgtcgac gcccatggca ccggcggcaa ctggatcgcc 120
ctcccccaca agatcgggct gaacaggtgc ggcaagagct gccggctgcg gtggctcaac 180
tacctccggc cgaacatccg gcacggcggc ttcaccgagg acgaggaccg cctcatctgc 240
agcctctaca tcgccatcgg gagcaggtgg gcgaccatcg cggcgcagct gccggggagg 300
acggacaacg acatcaagaa ctactggaac agcaagctca agcgccgcct gctcggcggc 360
ggccgccggc cgcggggcgc gccaccgcgg ctcgtgctcg ccggcccggg ccccgctgta 420
accgccgccg ccacgtcgcg caacgccatg gccgcgtcgg cgatcgagcg gatgcagctc 480
agcgtgcggc tgcgccgcct ggaggccgcg gcgccgccgc cgccgcagcc cttcaccttc 540
tacggcagca acaacctcgc cgcgccgccg tggcagcagc ctatctcccc ggccgcgggc 600
ggcagctcgg agatgccacg gcggctgcat caccaccacc cctccggcgc cgccgctacc 660
tcgagctact ccggcctgat cagcagctgg ccgtcgtctc gctcccacat catccacgac 720
gcctggctcg acgcctcctc caccccgccg ctgtcgacga cgagcatggg cgacgccgcc 780
acgacgacga cgacggccgg cggggagagc tcgagctcga cgccgacggt gagcacggcc 840
accacgccgt tcatcggcgg cagcatcgac atggacgacg agatcgacat gctgctccag 900
cagatcaggt gcttcgatga gaacggcgac gacggcgacg acgacgccga ccagcggctg 960
atcgtcggcg acgaggccgc agccggagca gagaactacc tcagggcatt gatagacgag 1020
gcggcagcga acggtggcga tgtcggcgtt ggctcctgga gctcttgctc tactccagga 1080
gtggactccg tgttccatga gtatgctcag ctagactacg gacagtataa ttaa 1134
<210>189
<211>377
<212>PRT
<213>水稻
<400>189
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ala Pro Glu Glu Asp Ala Ala Leu Lys Ala Tyr Val Asp Ala His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Thr Glu Asp Glu Asp Arg Leu Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ala Ile Gly Ser Arg Trp Ala Thr Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Ser Lys
100 105 110
Leu Lys Arg Arg Leu Leu Gly Gly Gly Arg Arg Pro Arg Gly Ala Pro
115 120 125
Pro Arg Leu Val Leu Ala Gly Pro Gly Pro Ala Val Thr Ala Ala Ala
130 135 140
Thr Ser Arg Asn Ala Met Ala Ala Ser Ala Ile Glu Arg Met Gln Leu
145 150 155 160
Ser Val Arg Leu Arg Arg Leu Glu Ala Ala Ala Pro Pro Pro Pro Gln
165 170 175
Pro Phe Thr Phe Tyr Gly Ser Asn Asn Leu Ala Ala Pro Pro Trp Gln
180 185 190
Gln Pro Ile Ser Pro Ala Ala Gly Gly Ser Ser Glu Met Pro Arg Arg
195 200 205
Leu His His His His Pro Ser Gly Ala Ala Ala Thr Ser Ser Tyr Ser
210 215 220
Gly Leu Ile Ser Ser Trp Pro Ser Ser Arg Ser His Ile Ile His Asp
225 230 235 240
Ala Trp Leu Asp Ala Ser Ser Thr Pro Pro Leu Ser Thr Thr Ser Met
245 250 255
Gly Asp Ala Ala Thr Thr Thr Thr Thr Ala Gly Gly Glu Ser Ser Ser
260 265 270
Ser Thr Pro Thr Val Ser Thr Ala Thr Thr Pro Phe Ile Gly Gly Ser
275 280 285
Ile Asp Met Asp Asp Glu Ile Asp Met Leu Leu Gln Gln Ile Arg Cys
290 295 300
Phe Asp Glu Asn Gly Asp Asp Gly Asp Asp Asp Ala Asp Gln Arg Leu
305 310 315 320
Ile Val Gly Asp Glu Ala Ala Ala Gly Ala Glu Asn Tyr Leu Arg Ala
325 330 335
Leu Ile Asp Glu Ala Ala Ala Asn Gly Gly Asp Val Gly Val Gly Ser
340 345 350
Trp Ser Ser Cys Ser Thr Pro Gly Val Asp Ser Val Phe His Glu Tyr
355 360 365
Ala Gln Leu Asp Tyr Gly Gln Tyr Asn
370 375
<210>190
<211>885
<212>DNA
<213>水稻
<400>190
atgaagaagg gaaaatggtc caaggaagag gacgatttga tcaaaaacca catggagaag 60
tatggcattg gccgtagctg gcaggcactg tctgatgctt tagggctgca gaggtgtggc 120
cggagctgcc gttcccggtg gctgaactac cttcggccgg ggctgaagca cggcgacttc 180
tcgccggcgg aggagaggat catctgcaag atgtacagca agaagggaag cagctggtcg 240
gccatcgccg cgcagctgcc ggggaggacg gacctcgccg tcaagaacta ctggaacagc 300
acgctcaaga agaggttccc ggcggcggcg gcggcgagga gcaccgccgc cgcgcgccgc 360
aggcaccgcc ccgccgccag cgccaccaca tcgtccgacg acgacgacga cgtcgacgtc 420
gacgacgcga ccccgcccgg cctcgcgctg gtcgtctaca gcgaggggag caccgccgcc 480
gcggcggcgg ccggcgagct cgctccgtac tccatctcat ccccggccgc cactgccgac 540
gcggcggaag aagaagagcc gatcgcggcc gttccgatca gcacctgcat cctggcactg 600
ccgccgccac caccaccgcc accgccgccg ccgagcgatg ccaccggcgg cgaggtgagc 660
atcccctgct tccccttctc gccgctgcca ttcatcgagc cggacttgcc ggagctgacc 720
tggacgaccg acctcgacga cattaccgcc accttcgatg ccgccgcctg ccgctacccg 780
aagcggccgc atccaatcac accggattcg cctcgccttc cgaagcttcc cgccattgcc 840
ggcttcgacg acgtgcagag cttcttgtct tggttcgatg actga 885
<210>191
<211>294
<212>PRT
<213>水稻
<400>191
Met Lys Lys Gly Lys Trp Ser Lys Glu Glu Asp Asp Leu Ile Lys Asn
1 5 10 15
His Met Glu Lys Tyr Gly Ile Gly Arg Ser Trp Gln Ala Leu Ser Asp
20 25 30
Ala Leu Gly Leu Gln Arg Cys Gly Arg Ser Cys Arg Ser Arg Trp Leu
35 40 45
Asn Tyr Leu Arg Pro Gly Leu Lys His Gly Asp Phe Ser Pro Ala Glu
50 55 60
Glu Arg Ile Ile Cys Lys Met Tyr Ser Lys Lys Gly Ser Ser Trp Ser
65 70 75 80
Ala Ile Ala Ala Gln Leu Pro Gly Arg Thr Asp Leu Ala Val Lys Asn
85 90 95
Tyr Trp Asn Ser Thr Leu Lys Lys Arg Phe Pro Ala Ala Ala Ala Ala
100 105 110
Arg Ser Thr Ala Ala Ala Arg Arg Arg His Arg Pro Ala Ala Ser Ala
115 120 125
Thr Thr Ser Ser Asp Asp Asp Asp Asp Val Asp Val Asp Asp Ala Thr
130 135 140
Pro Pro Gly Leu Ala Leu Val Val Tyr Ser Glu Gly Ser Thr Ala Ala
145 150 155 160
Ala Ala Ala Ala Gly Glu Leu Ala Pro Tyr Ser Ile Ser Ser Pro Ala
165 170 175
Ala Thr Ala Asp Ala Ala Glu Glu Glu Glu Pro Ile Ala Ala Val Pro
180 185 190
Ile Ser Thr Cys Ile Leu Ala Leu Pro Pro Pro Pro Pro Pro Pro Pro
195 200 205
Pro Pro Pro Ser Asp Ala Thr Gly Gly Glu Val Ser Ile Pro Cys Phe
210 215 220
Pro Phe Ser Pro Leu Pro Phe Ile Glu Pro Asp Leu Pro Glu Leu Thr
225 230 235 240
Trp Thr Thr Asp Leu Asp Asp Ile Thr Ala Thr Phe Asp Ala Ala Ala
245 250 255
Cys Arg Tyr Pro Lys Arg Pro His Pro Ile Thr Pro Asp Ser Pro Arg
260 265 270
Leu Pro Lys Leu Pro Ala Ile Ala Gly Phe Asp Asp Val Gln Ser Phe
275 280 285
Leu Ser Trp Phe Asp Asp
290
<210>192
<211>807
<212>DNA
<213>云杉属
<400>192
atgggaagag cgccctgttg tgacaaggca aatgtcaaaa agggaccatg gtcgccagaa 60
gaagacgcaa aactcaaggc ctttatcgag caacatggca ctggtggcaa ttggattgct 120
cttccacaga aagctggtct gaaaaggtgt ggaaaaagct gcaggcttag atggttgaac 180
tatttgaggc cagatataag gcatggtggt ttctcagagg atgaagatag catcatttgt 240
ggcctctatg caagcattgg aagcaggtgg tccataattg cagcccagtt accgggaaga 300
acggacaatg acatcaaaaa ctactggaat acaagactga agaaaaaact gcctgggaag 360
cgtaaagagc agcaaacacg taggtttaaa gaagcaaaga gcatgggtaa tggggcaggg 420
ccttatgttt cagaaggtat gtctgctgca tcatccacca taaatgcaat tagatctctg 480
atgtcaaaca cagaggcact ttatctgtcc gatcgaatgc ctcatatgga tatggatcca 540
tcgttaggcc tacttaatcc tcagttcttg cagcatactg tatcaaatat tgctaattat 600
tgctcaagct cggagcatgt tagctttgga actggtcctc cccaggattg gatcaggagc 660
ttacatttga caatgcaagt cttagaaagc tgctcttcag gactgaatgc gccttcactg 720
cagacaataa caataatacc tctcaagcat ctcccgcaat cattagttct cagatatatt 780
ccccatctac tattttggat cccgggc 807
<210>193
<211>269
<212>PRT
<213>云杉属
<400>193
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Phe Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Ser Glu Asp Glu Asp Ser Ile Ile Cys
65 70 75 80
Gly Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Pro Gly Lys Arg Lys Glu Gln Gln Thr Arg Arg
115 120 125
Phe Lys Glu Ala Lys Ser Met Gly Asn Gly Ala Gly Pro Tyr Val Ser
130 135 140
Glu Gly Met Ser Ala Ala Ser Ser Thr Ile Asn Ala Ile Arg Ser Leu
145 150 155 160
Met Ser Asn Thr Glu Ala Leu Tyr Leu Ser Asp Arg Met Pro His Met
165 170 175
Asp Met Asp Pro Ser Leu Gly Leu Leu Asn Pro Gln Phe Leu Gln His
180 185 190
Thr Val Ser Asn Ile Ala Asn Tyr Cys Ser Ser Ser Glu His Val Ser
195 200 205
Phe Gly Thr Gly Pro Pro Gln Asp Trp Ile Arg Ser Leu His Leu Thr
210 215 220
Met Gln Val Leu Glu Ser Cys Ser Ser Gly Leu Asn Ala Pro Ser Leu
225 230 235 240
Gln Thr Ile Thr Ile Ile Pro Leu Lys His Leu Pro Gln Ser Leu Val
245 250 255
Leu Arg Tyr Ile Pro His Leu Leu Phe Trp Ile Pro Gly
260 265
<210>194
<211>495
<212>DNA
<213>云杉属
<400>194
atgggccgag caccctgctg cgacaaagca aatgtcaaga gagggccgtg gtctcctgaa 60
gaggacacca tactcaaaaa tttcgtagag aagcatggca ccggaggcaa ctggatcgct 120
ttgcctcgca aagcaggttt gaaaaggtgc ggcaagagct gtagactgag gtggttgaat 180
tatttgaggc ctgatatcaa gcacggtgac ttctcagaag aagaagacag catcatttgc 240
agcctctata ccagcattgg aagcagatgg tctatcattg cagcccagtt gcccgggaga 300
acagacaacg acataaagaa ctactggaac acgcggctga agaaaaaatt gcttggcaaa 360
tgcaccaagg acaacgataa tcagcaaatc cggcgcttac tggcaaaaga agcagccaaa 420
ggtgcgggaa ttaggagacc ttacaacggt gatcatattt cttcagaaac ggccgccatg 480
tctgcgtcaa acact 495
<210>195
<211>165
<212>PRT
<213>云杉属
<400>195
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Ile Leu Lys Asn Phe Val Glu Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Arg Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Ser Ile Ile Cys
65 70 75 80
Ser Leu Tyr Thr Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Cys Thr Lys Asp Asn Asp Asn Gln
115 120 125
Gln Ile Arg Arg Leu Leu Ala Lys Glu Ala Ala Lys Gly Ala Gly Ile
130 135 140
Arg Arg Pro Tyr Asn Gly Asp His Ile Ser Ser Glu Thr Ala Ala Met
145 150 155 160
Ser Ala Ser Asn Thr
165
<210>196
<211>827
<212>DNA
<213>松属
<400>196
atgggaagag caccctgttg tgacaaggca aatgtcaaaa aaggatcttg gtcaccagaa 60
gaagacgcaa aactcaaggc gtttattgaa cagcatggca ctggtggcaa ttggattgct 120
cttccacaga aagctggtct gaaaaggtgt ggaaagagct gcaggcttag atggttgaac 180
tatttgaggc cagatataag gcatggtggt ttctcagaag atgaagataa catcatttgt 240
agcctctatg caagcattgg aagcaggtgg tctataattg cagcccagtt acctggaaga 300
acagacaatg acataaaaaa ttactggaac acaaggctga agaaaaagtt gcttgggaaa 360
cgtaaagatc agcaaacacg taggtttaaa gaagcaaaga acatgagtaa tggcacaggg 420
ccttatgttt cagaaggtat gtctactaca tcatccacca tcaatgcaat tagatctctg 480
atgtcaaaca ccgaggcact ttatctgtca gatcgaatgc ctcatatgga tatcgatcca 540
tcatcattgg gcctgcttaa tcctcaggtc ttgcagcata ctgtatcaaa tattgctaat 600
tattgctcaa gctcagagca tattagcttt ggaactggtc ctccccaggg actggatcag 660
gagcttacat ttgacaatgc aagtctgaga aagctgctct ttaggactga gtgtggcttc 720
actgcagaaa ataacaataa cagtgtctct caagcatctc caccaatcat tagttctcag 780
atatattccc catctactat tttggatccc ggacttgtcc atgactc 827
<210>197
<211>276
<212>PRT
<213>松属
<400>197
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Ser
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Phe Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Ser Glu Asp Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Arg Lys Asp Gln Gln Thr Arg Arg
115 120 125
Phe Lys Glu Ala Lys Asn Met Ser Asn Gly Thr Gly Pro Tyr Val Ser
130 135 140
Glu Gly Met Ser Thr Thr Ser Ser Thr Ile Asn Ala Ile Arg Ser Leu
145 150 155 160
Met Ser Asn Thr Glu Ala Leu Tyr Leu Ser Asp Arg Met Pro His Met
165 170 175
Asp Ile Asp Pro Ser Ser Leu Gly Leu Leu Asn Pro Gln Val Leu Gln
180 185 190
His Thr Val Ser Asn Ile Ala Asn Tyr Cys Ser Ser Ser Glu His Ile
195 200 205
Ser Phe Gly Thr Gly Pro Pro Gln Gly Leu Asp Gln Glu Leu Thr Phe
210 215 220
Asp Asn Ala Ser Leu Arg Lys Leu Leu Phe Arg Thr Glu Cys Gly Phe
225 230 235 240
Thr Ala Glu Asn Asn Asn Asn Ser Val Ser Gln Ala Ser Pro Pro Ile
245 250 255
Ile Ser Ser Gln Ile Tyr Ser Pro Ser Thr Ile Leu Asp Pro Gly Leu
260 265 270
Val His Asp Ser
275
<210>198
<211>1245
<212>DNA
<213>松属
<400>198
atgggtcgag caccctgctg tgacaaagca aatgtcaaga gagggccatg gtctcctgaa 60
gaggacacca tactaaaaaa tttcgtggag aagcatggca ctggaggcaa ctggatcgct 120
ttgcctcgca aagcaggttt aaaaaggtgt ggcaagagtt gcaggctgag atggctgaat 180
tatttgaggc ctgatattaa gcatggtgac ttctcagaag aagaagacga catcatttgc 240
accctgtata ccagcattgg aagcagatgg tctatcattg cagcccagtt gccagggcga 300
acagacaacg acataaagaa ctactggaac acgcggctga agaaaaaatt gcttggcaaa 360
tgcagcaagg acaacgataa tcagcaaatc cgacgcctac tggcaaaaga agcagctaaa 420
ggcgcgggaa ttagaggact ttacaacggt gatcataata tttcttcaga aacggccgcc 480
atttctgcat caaacactca aagttcgtca gaatctctga cagaaacagc cactgccgca 540
aatgccatga atagccctta taatgcccta gaaacgggta aagtcccggg atcgaattcg 600
tctgagtcaa aagcggccgg ttttaatgcc acccacagtc ctaataccca ttattatgaa 660
caaggtttca gtcaaatcat gtctcaggca gatcagtatt cttccctgac acacatgctt 720
ctgagactcg agaataacga gagcgattgc tcaacagaca atattcaatc tctgggcatc 780
gatacaattc ccagtgaggt ccctttctac gcttccacgg ccatgaatgt aaaagctgaa 840
gccatggagc gcccatctag tgatccccag ctcaatcaag cccgcaattc agtgcctgca 900
ctctgggaaa cctgtaccag caattccaca tctgggggca acaattatca gttcagtacg 960
ttgataaacg atgatacggg caatttcagc tatgggctct cagcggacat ggatgagttc 1020
atagtgtatc gaaattatgg aggcaatggc tcaatatctc aggtgaaaga ggagcctgac 1080
tactccacag ctgaagccta ctgggcttct caactagctg aacctgcaaa gtcctcaggc 1140
ttaacaacaa catgtcccaa ttatgcatac attttgccat catctgaggg tggtatgggt 1200
tctggaacta ccccacaggg gctctttcag gagggaatta tctat 1245
<210>199
<211>415
<212>PRT
<213>松属
<400>199
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Thr Ile Leu Lys Asn Phe Val Glu Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Arg Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Asp Ile Ile Cys
65 70 75 80
Thr Leu Tyr Thr Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Cys Ser Lys Asp Asn Asp Asn Gln
115 120 125
Gln Ile Arg Arg Leu Leu Ala Lys Glu Ala Ala Lys Gly Ala Gly Ile
130 135 140
Arg Gly Leu Tyr Asn Gly Asp His Asn Ile Ser Ser Glu Thr Ala Ala
145 150 155 160
Ile Ser Ala Ser Asn Thr Gln Ser Ser Ser Glu Ser Leu Thr Glu Thr
165 170 175
Ala Thr Ala Ala Asn Ala Met Asn Ser Pro Tyr Asn Ala Leu Glu Thr
180 185 190
Gly Lys Val Pro Gly Ser Asn Ser Ser Glu Ser Lys Ala Ala Gly Phe
195 200 205
Asn Ala Thr His Ser Pro Asn Thr His Tyr Tyr Glu Gln Gly Phe Ser
210 215 220
Gln Ile Met Ser Gln Ala Asp Gln Tyr Ser Ser Leu Thr His Met Leu
225 230 235 240
Leu Arg Leu Glu Asn Asn Glu Ser Asp Cys Ser Thr Asp Asn Ile Gln
245 250 255
Ser Leu Gly Ile Asp Thr Ile Pro Ser Glu Val Pro Phe Tyr Ala Ser
260 265 270
Thr Ala Met Asn Val Lys Ala Glu Ala Met Glu Arg Pro Ser Ser Asp
275 280 285
Pro Gln Leu Asn Gln Ala Arg Asn Ser Val Pro Ala Leu Trp Glu Thr
290 295 300
Cys Thr Ser Asn Ser Thr Ser Gly Gly Asn Asn Tyr Gln Phe Ser Thr
305 310 315 320
Leu Ile Asn Asp Asp Thr Gly Asn Phe Ser Tyr Gly Leu Ser Ala Asp
325 330 335
Met Asp Glu Phe Ile Val Tyr Arg Asn Tyr Gly Gly Asn Gly Ser Ile
340 345 350
Ser Gln Val Lys Glu Glu Pro Asp Tyr Ser Thr Ala Glu Ala Tyr Trp
355 360 365
Ala Ser Gln Leu Ala Glu Pro Ala Lys Ser Ser Gly Leu Thr Thr Thr
370 375 380
Cys Pro Asn Tyr Ala Tyr Ile Leu Pro Ser Ser Glu Gly Gly Met Gly
385 390 395 400
Ser Gly Thr Thr Pro Gln Gly Leu Phe Gln Glu Gly Ile Ile Tyr
405 410 415
<210>200
<211>681
<212>DNA
<213>枸橘
<400>200
atgggtaggg ctccttgctg tgacaaggca aatgtgaaga aggggccatg gtcacctgaa 60
gaagactcaa agcttaagga gtacatagag aaatatggca ctggtggcaa ctggattgct 120
cttcctcaga aagctggtct aaagagatgt gggaaaagtt gcagactgag atggctcaac 180
tatttgaggc caaatattaa acatggggaa tttactgatg aggaagatag gctgatctgt 240
agcctctttg ctagcattgg aagcagatgg tcaattatag ctgctcagct accgggtagg 300
actgataatg atatcaaaaa ctactggaac acaaagctca agaagaaact catggccatg 360
gctcctccat tatcatcaca aaagaagagc actgctccac cactgatccc atcatctcat 420
catcatcacc aagctttagt gtcactacta ccatctcaac cctattacac cccttctaat 480
aaatctctta tcagtaactc ttttgattct tttgaaccca tttcatcaaa caccccatta 540
catctttttg acaacaacaa cttgatccaa atccaagaaa accagctgtt tagtaataat 600
aattccatgc agggttacta tccaatgaaa gacaatttct tgacttttgg tagcgagcaa 660
agttgcagtt cttctgatgg t 681
<210>201
<211>227
<212>PRT
<213>枸橘
<400>201
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ser Lys Leu Lys Glu Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Thr Asp Glu Glu Asp Arg Leu Ile Cys
65 70 75 80
Ser Leu Phe Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Ala Met Ala Pro Pro Leu Ser Ser Gln Lys
115 120 125
Lys Ser Thr Ala Pro Pro Leu Ile Pro Ser Ser His His His His Gln
130 135 140
Ala Leu Val Ser Leu Leu Pro Ser Gln Pro Tyr Tyr Thr Pro Ser Asn
145 150 155 160
Lys Ser Leu Ile Ser Asn Ser Phe Asp Ser Phe Glu Pro Ile Ser Ser
165 170 175
Asn Thr Pro Leu His Leu Phe Asp Asn Asn Asn Leu Ile Gln Ile Gln
180 185 190
Glu Asn Gln Leu Phe Ser Asn Asn Asn Ser Met Gln Gly Tyr Tyr Pro
195 200 205
Met Lys Asp Asn Phe Leu Thr Phe Gly Ser Glu Gln Ser Cys Ser Ser
210 215 220
Ser Asp Gly
225
<210>202
<211>423
<212>DNA
<213>杨属
<400>202
atggggaggg ctccttgctg cgacaaagcc aacgtcaaga aaggcccatg gtcacctgaa 60
gaagatgcga agctcaagtc atatatagag cagcatggca ctggtggtaa ctggattgct 120
ttgcctcaaa aaattggtct taagagatgc ggcaagagct gccgccttag atggttaaat 180
tatcttcgcc cgaatattaa gcacggaggc ttctctgaag aagaagataa cataatttgc 240
agcctttaca ttagtatcgg aagcagatgg tccataattg cagcacaatt gccaggaaga 300
accgataatg atataaagaa ctactggaac acaaggctga agaagaagct tcttggcaag 360
cagcgaaaag agcaacaggc tcgcagaagt agtggcctaa agcaagaaat gaagagagga 420
aat 423
<210>203
<211>345
<212>PRT
<213>杨属
<400>203
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Gln Ala Arg
115 120 125
Arg Ser Ser Gly Leu Lys Gln Glu Met Lys Arg Gly Asn Gly Asn Pro
130 135 140
Val Val Pro Ala Asp Asn Asn Asn Gln Asn Pro Tyr Trp Pro Glu Leu
145 150 155 160
Pro Ile Leu Ala Pro Ile Pro Tyr Ser Asn His Glu Pro His Phe Asn
165 170 175
Asp His Ala Ser Ile Arg Lys Leu Leu Ile Lys Leu Gly Gly Lys Phe
180 185 190
Ser Asp Asp Asp Gln Val Asn His Asn Ala Met Asn Pro Gln Phe Pro
195 200 205
Ala Asp Val Ser Tyr Thr Gln Gln Leu Tyr Asp Asp Gln Ser Ile Asn
210 215 220
Val Ser Ser Ser Ala Pro Met Glu Glu Thr Leu Asp Asp Thr Asp Thr
225 230 235 240
Gln Phe Ala Gln Thr Gln Tyr Asp Ile Asp Gly Ala Ala Gly Leu Gln
245 250 255
Met Leu Gln Gly Gln Ser Ser Phe Pro Ala Gly Leu Glu Gln Met Val
260 265 270
Ser Ser Asn Pro Pro Arg Leu Asp Gly Leu Glu Phe Leu Leu Gly Asp
275 280 285
Asp Met Val Asn Asn Arg Ile Arg Thr Ala Tyr Gly Thr Glu Ser Met
290 295 300
Ile Asp Cys Gly Glu Met Thr Ser Leu Ile Phe Pro Pro Gly Ala Ser
305 310 315 320
Asn Cys Glu Gly Ile Ile Gln Gln Arg Leu Leu Gln Glu Cys Ala Phe
325 330 335
Asp Glu Pro Arg Tyr Pro Gly Pro Leu
340 345
<210>204
<211>572
<212>DNA
<213>杨属
<400>204
atggggagag ctccttgctg tgacaaagcc aacgtgaaga aaggtccatg gtctcccgag 60
gaagatgcca tactcaaggc ctatattgag cagcatggaa cgggtggcaa ttggattgcc 120
ttgccacaga agataggcct gaaaaggtgt ggcaagagtt gtcgacttag atggttgaat 180
tatctccgtc caaacattaa acatggaggg ttttcagagg aagaagataa cattatttgc 240
agtctctata taagtattgg aagcaggtgg tctatcattg ctgctcagtt acctggaaga 300
accgataatg atataaaaaa ctactggaac acaaggctta agaagaagct cttaggaaaa 360
cagcgcaagg aacaagcagc ccggcgagct agtctcaagc aagaaatcat gacaaagaga 420
gaaataaatg agagcttcat ggttcctggg gctatccctc atcaacaaag cccttactgg 480
ccagaggtac cagctctagt catgaatcaa aaccaagatt ctcatttgat ggatcaagaa 540
tccattagga acttgctgat caaacttggt gg 572
<210>205
<211>191
<212>PRT
<213>杨属
<400>205
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Ile Leu Lys Ala Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Ala Ala Arg
115 120 125
Arg Ala Ser Leu Lys Gln Glu Ile Met Thr Lys Arg Glu Ile Asn Glu
130 135 140
Ser Phe Met Val Pro Gly Ala Ile Pro His Gln Gln Ser Pro Tyr Trp
145 150 155 160
Pro Glu Val Pro Ala Leu Val Met Asn Gln Asn Gln Asp Ser His Leu
165 170 175
Met Asp Gln Glu Ser Ile Arg Asn Leu Leu Ile Lys Leu Gly Gly
180 185 190
<210>206
<211>999
<212>DNA
<213>杨属
<400>206
atgggaaggg ctccttgttg tgacaaggct aatatgaaga aagggccctg gccgcctgaa 60
gaagatgcaa agctgaagga gtacttggaa aaacaaggga ctggagggaa ttggattgct 120
ctcccacaaa aggctggtca taaaagatgt gggaaaagct gcagattaag atggctaaac 180
tatctcaggc ccaacattaa acatggagag ttctctgatg atgaagacag gataatctgc 240
agcctatatg ccaacattgg aagccggtgg tcaataatag cagctcagtt gccaggcagg 300
acggataatg acataaaaaa ctactggaac accaagctca agaagaaact aatgggtgtg 360
attaatccta ttgctcagag aaaacctcag caagctgctc ttttttcatc tctccttcaa 420
gctacatcat caccgtcttc accatccact ctcctgtcat caccatcatc atcattcaca 480
tgcagcaaca acagttatta cagtaaccta actaggtctt tcactgatcc aatctcattt 540
tcatcaagtc ctatgagcac tagctctttc gctactgctt ccatgctaca cccccaacaa 600
acctttgtgg ggcctatgca aaatgatcaa gttaaagata gtcttataat gtttggaggt 660
gaagccagct gcagctcgtc tgatgggagt tgcaacaacc agatgagcca tgtcaaagag 720
gagtatgaat atagtggtgg tacaaataac aacaatgaac agatggggtt acaaaattat 780
ccctacaatg gggttgaaga tggccaaaaa ttgatggttt caagtgaggc tgctgctcat 840
ggtgtactta atgggtggat tgagaagcaa aatgggttat ggccgggaga taactcacta 900
gactatggtc tagaggagat taagcaactt attagcacta gcagctgtaa tagctttttg 960
tttgatgaaa acaagacagg aggaaaagtc atgtactac 999
<210>207
<211>333
<212>PRT
<213>杨属
<400>207
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Met Lys Lys Gly Pro
1 5 10 15
Trp Pro Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Leu Glu Lys Gln
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly His Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Val Ile Asn Pro Ile Ala Gln Arg Lys
115 120 125
Pro Gln Gln Ala Ala Leu Phe Ser Ser Leu Leu Gln Ala Thr Ser Ser
130 135 140
Pro Ser Ser Pro Ser Thr Leu Leu Ser Ser Pro Ser Ser Ser Phe Thr
145 150 155 160
Cys Ser Asn Asn Ser Tyr Tyr Ser Asn Leu Thr Arg Ser Phe Thr Asp
165 170 175
Pro Ile Ser Phe Ser Ser Ser Pro Met Ser Thr Ser Ser Phe Ala Thr
180 185 190
Ala Ser Met Leu His Pro Gln Gln Thr Phe Val Gly Pro Met Gln Asn
195 200 205
Asp Gln Val Lys Asp Ser Leu Ile Met Phe Gly Gly Glu Ala Ser Cys
210 215 220
Ser Ser Ser Asp Gly Ser Cys Asn Asn Gln Met Ser His Val Lys Glu
225 230 235 240
Glu Tyr Glu Tyr Ser Gly Gly Thr Asn Asn Asn Asn Glu Gln Met Gly
245 250 255
Leu Gln Asn Tyr Pro Tyr Asn Gly Val Glu Asp Gly Gln Lys Leu Met
260 265 270
Val Ser Ser Glu Ala Ala Ala His Gly Val Leu Asn Gly Trp Ile Glu
275 280 285
Lys Gln Asn Gly Leu Trp Pro Gly Asp Asn Ser Leu Asp Tyr Gly Leu
290 295 300
Glu Glu Ile Lys Gln Leu Ile Ser Thr Ser Ser Cys Asn Ser Phe Leu
305 310 315 320
Phe Asp Glu Asn Lys Thr Gly Gly Lys Val Met Tyr Tyr
325 330
<210>208
<211>387
<212>DNA
<213>无梗花栎
<400>208
atgggaagag ctccttgttg tgataaggcc aacgtgaaaa aagggccatg gtcacctgaa 60
gaagatgcaa agctgaaaga gtatatagat aaatatggga ctggagggaa ttggattgct 120
cttccacaga aagcaggtct caagagatgt ggaaaaagcc gcagattaag atggcttaac 180
tatctcagac ccaacattaa acatggtgaa ttcactgaaa atgaagatag gataatatgc 240
agcctctttg ctaacattgg aagcaggtgg tcaataatag cagctcagtt gccaggcagg 300
acagacaatg accttaagaa ctattgtaac accaagctta agaagaaact catgggtata 360
tttcctccat ctcagagaaa aggtcac 387
<210>209
<211>129
<212>PRT
<213>无梗花栎
<400>209
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Asp Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Arg Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Thr Glu Asn Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Phe Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Leu Lys Asn Tyr Cys Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Ile Phe Pro Pro Ser Gln Arg Lys Gly
115 120 125
His
<210>210
<211>547
<212>DNA
<213>高山栎类
<400>210
accttgctct cctattctgg catgcttaat ttctggaatt ttctatacaa actttatgcc 60
acaccctcct taaaatatta attattggct gtttccctaa taaccatgta tatcttcaag 120
cttttataat cctctctctc tctctctctc tctctctctc tctctctcat attcttattt 180
gggtttcaga gggagaagct ataggctata gcatcaagct caaagcaaca ccatggggag 240
agctccatgt tgtgacaaag caaacgtcaa gaaaggccca tggtcacctg aagaagatgc 300
caagctcaag tcgtatatag agaagcatgg caccggtggt aactggatcg ctttaccaca 360
aaaaaattgg cctgaagaga tgcggcaaga gttgccgcct tagatggtta aactatctcc 420
gtccaaatat caagcatgga ggattttcag aagaggaaga taacataatt tgcagcctct 480
atattagtat tggaagcagg tggtctatta ttgcagcaca attgccagga agaacagata 540
atgatat 547
<210>211
<211>648
<212>DNA
<213>野萝卜
<400>211
atgggaaggg caccttgttg tgacaaagcc aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcca aactcaaaga ttacatcgag aatagtggta caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcaggctaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aagaagacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaact gccgggaaga 300
acggacaacg atatcaagaa ctactggaac acgaggctga agaagaagct tctaaacaaa 360
caaaggaaag agttccaaga agctcgaatg aagcaagaga tggtgatgat gaagaggcaa 420
caacaagtac aaaatggtag tacggatctt tatctgaaaa acatgtttgg atcatcatca 480
tggccattac tacaacagct tcctcatcat caagtacctc ttgtgatgat ggaaccaaca 540
aactgtaact actaccaaat gtcaccgtct tgcaacctag aacaaaagca acttatcgct 600
ctcaataaca tggtcaagat tgaagaagaa ccggagagaa caaaccct 648
<210>212
<211>216
<212>PRT
<213>野萝卜
<400>212
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Val Gln
130 135 140
Asn Gly Ser Thr Asp Leu Tyr Leu Lys Asn Met Phe Gly Ser Ser Ser
145 150 155 160
Trp Pro Leu Leu Gln Gln Leu Pro His His Gln Val Pro Leu Val Met
165 170 175
Met Glu Pro Thr Asn Cys Asn Tyr Tyr Gln Met Ser Pro Ser Cys Asn
180 185 190
Leu Glu Gln Lys Gln Leu Ile Ala Leu Asn Asn Met Val Lys Ile Glu
195 200 205
Glu Glu Pro Glu Arg Thr Asn Pro
210 215
<210>213
<211>846
<212>DNA
<213>野萝卜
<400>213
ggatactttt ctccattata cacattttca tgtatgtata tataaatcat cacatacacg 60
caccataaca ctaacatcct cctcctcttc atcatcaaca tagttgagaa agatcgaaaa 120
aaagagagag aaagagggat acatcaagaa caagaatcgc gaatcaagag gatgggaagg 180
gcaccgtgtt gtgataaggc taacgtgaag aaagggcctt ggtctcctga agaagatgca 240
aaactcaaag attacataga gagtagtggc acaggaggca actggatcgc tttgcctcag 300
aagattggtc taaggagatg tgggaagagt tgcagactaa ggtggctcaa ctatttaaga 360
ccaaacatca aacatggtgg cttctctgag gaagaggaca acatcatttg taacctctat 420
gttactattg gtagcaggtg gtctataata gctgcacaat tgcctggaag aacagacaat 480
gacatcaaga actattggaa cacgaggctg aagaagaagc ttcttaacaa acaaagaaaa 540
gagttccaag aagctcggat acagcagaga tggtgatgat gaaacgacaa caacaagaca 600
aggacaattc caaattaatg gtagtacgga tctttatctg aacaacatgt ttggatcatc 660
agcatggcca ttactacctc agcttcctcc tcctcatagt caagtacctc ttgtgatgat 720
ggaaccaaca agctgcaatt actaccaaac gacaccttct tgcacataga caaagcatga 780
tacactcaga cacgtcagat gagagacgag agaacaacct gatcatcatc accacgagac 840
tctatg 846
<210>214
<211>522
<212>DNA
<213>野萝卜
<400>214
atgggaaggg caccttgttg tgacaaagcc aacgtgaaga aagggccttg gtctcctgag 60
gaagatgcca aactcaaaga ttacatcgag aatagtggta caggaggcaa ctggatcgct 120
ttgcctcaga agattggtct aaggagatgt gggaagagtt gcaggctaag gtggctcaac 180
tatttgagac caaacatcaa acatggtggc ttctctgagg aagaagacaa catcatttgt 240
aacctctatg ttactattgg tagcaggtgg tctataattg ctgcacaact gccgggaaga 300
acggacaacg atatcaagaa ctactggaac acgaggctga agaagaagct tctaaacaaa 360
caaaggaaag agttccaaga agctcgaatg aagcaagaga tggtgatgat gaagaggcaa 420
caacaagtac aaaatggtag tacggatctt tatctgaaaa acatgtttgg atcatcatca 480
tggccattac tacaacagct tcctcatcat caagtacctc tt 522
<210>215
<211>174
<212>PRT
<213>野萝卜
<400>215
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Tyr Ile Glu Asn Ser
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Val Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Asn Lys Gln Arg Lys Glu Phe Gln Glu Ala
115 120 125
Arg Met Lys Gln Glu Met Val Met Met Lys Arg Gln Gln Gln Val Gln
130 135 140
Asn Gly Ser Thr Asp Leu Tyr Leu Lys Asn Met Phe Gly Ser Ser Ser
145 150 155 160
Trp Pro Leu Leu Gln Gln Leu Pro His His Gln Val Pro Leu
165 170
<210>216
<211>842
<212>DNA
<213>野萝卜
<400>216
tcttcaaaaa cgaaaccaag attcctcaaa cagaacacac agagtagcta taaggaccga 60
gagagatcgt agagagatgg ggagggctcc atgttgtgac aaagcaaatg tgaagagagg 120
tccatggtct ccggaagaag atgcaaagct taaagattac atagagaaac aaggcactgg 180
tggcaactgg attgctctcc ctcacaaagc tggtttaagg agatgtggga agagttgtag 240
actgaggtgg ttaaactatt tgaggccaaa cataagacat ggagatttct ctgaggaaga 300
agacaatatt atctgcagcc tctttgcctc cattggaaca ctaagctcaa gaagaagctc 360
attgccacta tggctcctcc accacctcat caactcgtag ccattgcctc atcatcatca 420
tcaccatcat catcacacta caacatgacc aataatcttc ctccgtataa cccaacaata 480
tctacaaacc agctgatctc acctcatctc tcacctcatc aggagatgat gatgacaatg 540
atggaccaac aacaacaact attatacaaa gaagccatgg acagtttggt aaattctcca 600
aatagcaaca agcttataat gagccatcaa gaagacagcc atgcgcaaag tacaaacaaa 660
ggaataatgt tgttgagtga tgtaagaagt gggtcaagca caacaagtac agtaacaaga 720
gtgaagatgg aacaacatga tcatcatcat gaagagagat caatgcaaga ttatggaatg 780
gaggagatca atcacttaat aagtagtagt tgtacgagta gtagtagcac agcttgtggt 840
tt 842
<210>217
<211>788
<212>DNA
<213>菜菔
<400>217
gctcctcatc aagtacctct tgtgatgatg gaaccgacaa actgtaacta ctaccaaacg 60
tcaccgtctt gcaacctaga acaaaagcaa ccgatcgctc tcaataacat ggtcaagatt 120
gaagaagaac cggagagaac aaaccctgat catcatcagc atcaagattc tatcacaaac 180
ccttttgatg tctccttctc tcagcttttg ttagatcctt attactactt aggatcagga 240
gaaggaggag gagaagggga ttttgctatc atgagtagca gcacaaattc tccattacca 300
aacacaagtg gtgatcaaaa tgaacatcag cagcaagaga tttttcaatg gtttgggagt 360
agtaatcttc agacagaagc aagcagcgat atgttcttaa acaacaacat agcgaatctt 420
gagaccaacg agggcacaaa attctactca tcattagctg gcgctggggc ggctttggcc 480
gaaggaacga cgagtacatc cgcagatcaa agcacaataa gttgggagga cataacttct 540
cttgttaatt cagaagatgc aagttacttc aatgggccaa attagttatg tgcatttttc 600
aataaacatt tgactttttg aggtgtttca ttttgtataa ttatgtatca ttagcgggtc 660
ggccgattaa ttatatggat tgagattaat gttcatgcag agatttgttt agtttagttt 720
caacttagct ttatgtacga accttttttt taacaccatt cacgacatat atatgagtga 780
agagatct 788
<210>218
<211>731
<212>DNA
<213>月季
<400>218
atgggaagag ctccatgttg tgacaaagct agtgtcaaaa gggggcaatg gtctcctgag 60
gaagatgaag ctctcaagac ttaccttgag actcatggaa ctggtggcaa ttggattcat 120
ctgcctcaaa aagctgggct taggagatgt gggaagagct gccgtctgcg gtggttgaac 180
tatctgaggc cagacatcaa acatggaggc ttcactgaag aggaggacaa cattatctgt 240
aacctctaca ctcaaatggg aagccgatgg tcatacatag ctgctcaaat gcctggaaga 300
acagacaatg atgtgaagaa ctattggaac accaaattga agaagaggtt ttttagcaaa 360
aacccaaatg cctcctcact cacactgcct tgtgttccaa aacttgaaac caatgatgag 420
tttcagaccc aaatacctcc cacattgatg ttatctgatt ataattctgg attgaatgcc 480
tatgatcaaa gtttgagctt aaagccaaat ccattacaca actccaagct cacgggctta 540
tcacaatttg gtgcaagttc tgtttcaatg tctcaagatg gttctagtgt ttcagattcg 600
tcttcaattg ctgcagttga aaatggttta ctacatggaa atagcttcat aaatgaggat 660
gctgctggga tacccatgga ttttggattt ggaggattgc cttatgatgt tgtcaatcag 720
atgtggtttg g 731
<210>219
<211>244
<212>PRT
<213>月季
<400>219
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Gln
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Ala Leu Lys Thr Tyr Leu Glu Thr His
20 25 30
Gly Thr Gly Gly Asn Trp Ile His Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Asn Leu Tyr Thr Gln Met Gly Ser Arg Trp Ser Tyr Ile Ala Ala Gln
85 90 95
Met Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Phe Phe Ser Lys Asn Pro Asn Ala Ser Ser Leu Thr
115 120 125
Leu Pro Cys Val Pro Lys Leu Glu Thr Asn Asp Glu Phe Gln Thr Gln
130 135 140
Ile Pro Pro Thr Leu Met Leu Ser Asp Tyr Asn Ser Gly Leu Asn Ala
145 150 155 160
Tyr Asp Gln Ser Leu Ser Leu Lys Pro Asn Pro Leu His Asn Ser Lys
165 170 175
Leu Thr Gly Leu Ser Gln Phe Gly Ala Ser Ser Val Ser Met Ser Gln
180 185 190
Asp Gly Ser Ser Val Ser Asp Ser Ser Ser Ile Ala Ala Val Glu Asn
195 200 205
Gly Leu Leu His Gly Asn Ser Phe Ile Asn Glu Asp Ala Ala Gly Ile
210 215 220
Pro Met Asp Phe Gly Phe Gly Gly Leu Pro Tyr Asp Val Val Asn Gln
225 230 235 240
Met Trp Phe Gly
<210>220
<211>362
<212>DNA
<213>甘蔗
<220>
<221>其他特征
<222>(207)..(207)
<220>
<221>其他特征
<222>(207)..(207)
<223>n是任意核苷
<400>220
atggggcgcg cgccgtgctg cgacaaggcg agcgtgaagc gggggccctg gtcgcccgag 60
gaggacgagc agctgcggag ctacgtccag cgcaacggca tcggcggcaa ctggatcgcc 120
ctgccgcaga aagcagggct gaaccggtgc ggcaagagct gccggctgcg gtggctcaac 180
tacctgcggc cggacatcaa gcacggnggc tacaccgagc aggaggaccg gatcatctgg 240
tcactctaca gctccatcgg aagcaggtgg tcgatcatcg cgtccaagct ccccggccgg 300
accgacaacg acgtcaagaa ctactggaac accaagctca agaagaaggc catggcggcg 360
gt 362
<210>221
<211>121
<212>PRT
<213>甘蔗
<400>221
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Gln Leu Arg Ser Tyr Val Gln Arg Asn
20 25 30
Gly Ile Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Tyr Thr Glu Gln Glu Asp Arg Ile Ile Trp
65 70 75 80
Ser Leu Tyr Ser Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ala Met Ala Ala Val
115 120
<210>222
<211>393
<212>DNA
<213>甘蔗
<220>
<221>其他特征
<222>(296)..(296)
<223>n是任意核苷
<220>
<221>其他特征
<222>(351)..(351)
<223>n是任意核苷
<400>222
atgggtcggg cgccgtgctg cgacaagacg agtgtgaagc gggggccgtg gtcgcccgag 60
gaggacgagc tgctgcggag ctacgtccac aaccacggca ccggcggcaa ctggatcgcg 120
ctcccgcaca aagcagggct gaaccggtgc ggcaagagct gccggctgcg gtggctcaac 180
tacctgcgcc cggacatcaa gcacggcggc tacacggagc aggaggaccg gatcatctgc 240
tccctctaca actccatcgg gagcaggtgg tccatcatcg cgtccaagct gccggngcgg 300
acggacaacg acgtcaagaa ctactgggac accaagctca agaagaaggc natcgccatg 360
caccaccacc agcagcagca gcagcaggag tac 393
<210>223
<211>131
<212>PRT
<213>甘蔗
<220>
<221>其他特征
<222>(99)..(99)
<223>X是任意氨基酸
<400>223
Met Gly Arg Ala Pro Cys Cys Asp Lys Thr Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Leu Leu Arg Ser Tyr Val His Asn His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Tyr Thr Glu Gln Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Asn Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Xaa Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asp Thr Lys
100 105 110
Leu Lys Lys Lys Ala Ile Ala Met His His His Gln Gln Gln Gln Gln
115 120 125
Gln Glu Tyr
130
<210>224
<211>726
<212>DNA
<213>甘蔗
<400>224
atggggcgga cgccgtgctg cgacagggcg gccgtgaagc agggcccgtg gtcgccggag 60
gaggacgagg cgctgcggag ctacgtccag cgccacggca gcggcggcaa ctggatcgcc 120
atgcccaaga aagccgggct caagaggtgc ggcaagagct gcaggctgcg ctggctcaac 180
tacctccgcc cggacatccg ccacggcggc ttcaccgccg aggaggacgc cgtcatcatg 240
tccctccaca cccagctcgg gagcaagtgg tcgctgatag cctcgcagat ggaagggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agaagcgcct cctcgccgcc 360
gccctgtccc cgttcccgcc acctcacgcc cggctgccgg cgcccgcgcc tgcttccagc 420
accgcgcacg cgtcgtcgct attcccttcg ctcgacatac cgaccgtgaa gacggaggcg 480
tacacctgcg acgacttcct ggcgccggcg ctccgcgacc cgttcgctgc cgccgccgac 540
ggctccacct cggtcgcctc cgcggcgtcg tcggcctcca actggtcgac ggcggacaac 600
ggcgccagcg aagcgtccct cttcctgctg gacttctgcg cgggccccga cctcggcgcc 660
gccgctgacc agctccagct ccccggcggc tactactacc ctctcgatcc aagcttgtcg 720
ccggta 726
<210>225
<211>242
<212>PRT
<213>甘蔗
<400>225
Met Gly Arg Thr Pro Cys Cys Asp Arg Ala Ala Val Lys Gln Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Ala Leu Arg Ser Tyr Val Gln Arg His
20 25 30
Gly Ser Gly Gly Asn Trp Ile Ala Met Pro Lys Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Ala Glu Glu Asp Ala Val Ile Met
65 70 75 80
Ser Leu His Thr Gln Leu Gly Ser Lys Trp Ser Leu Ile Ala Ser Gln
85 90 95
Met Glu Gly Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Leu Ala Ala Ala Leu Ser Pro Phe Pro Pro Pro
115 120 125
His Ala Arg Leu Pro Ala Pro Ala Pro Ala Ser Ser Thr Ala His Ala
130 135 140
Ser Ser Leu Phe Pro Ser Leu Asp Ile Pro Thr Val Lys Thr Glu Ala
145 150 155 160
Tyr Thr Cys Asp Asp Phe Leu Ala Pro Ala Leu Arg Asp Pro Phe Ala
165 170 175
Ala Ala Ala Asp Gly Ser Thr Ser Val Ala Ser Ala Ala Ser Ser Ala
180 185 190
Ser Asn Trp Ser Thr Ala Asp Asn Gly Ala Ser Glu Ala Ser Leu Phe
195 200 205
Leu Leu Asp Phe Cys Ala Gly Pro Asp Leu Gly Ala Ala Ala Asp Gln
210 215 220
Leu Gln Leu Pro Gly Gly Tyr Tyr Tyr Pro Leu Asp Pro Ser Leu Ser
225 230 235 240
Pro Val
<210>226
<211>413
<212>DNA
<213>黑麦
<400>226
atggggaggg cgccgtgctg cgacagggcg gcggtgaaga ggggcccgtg gtcgccggag 60
gaggacgacg cgctgcgcga ctacatgcag cgctacggaa acaccggcag ctggatcacg 120
ctccccatga gaggtgggct caagaggtgc ggcaagagct gcaggctgcg gtggctcaac 180
tacctccgcc ccgacatccg ccacggcggc ttcaccgacg aggaggacac catcatctac 240
tccctctaca gccagctggg cagcaagtgg tcgctgatag cgtcgcagct ggagaggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agaagcggct cgccgccgcg 360
gccgccgcct tctccacccg ccgttccccg tccttcatgc cgctaccggc gcc 413
<210>227
<211>138
<212>PRT
<213>黑麦
<400>227
Met Gly Arg Ala Pro Cys Cys Asp Arg Ala Ala Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Asp Ala Leu Arg Asp Tyr Met Gln Arg Tyr
20 25 30
Gly Asn Thr Gly Ser Trp Ile Thr Leu Pro Met Arg Gly Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Asp Glu Glu Asp Thr Ile Ile Tyr
65 70 75 80
Ser Leu Tyr Ser Gln Leu Gly Ser Lys Trp Ser Leu Ile Ala Ser Gln
85 90 95
Leu Glu Arg Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Ala Ala Ala Ala Ala Ala Phe Ser Thr Arg Arg
115 120 125
Ser Pro Ser Phe Met Pro Leu Pro Ala Pro
130 135
<210>228
<211>555
<212>DNA
<213>番茄
<400>228
atgggaagag ctccttgttg tgataagaat aatgttaaaa gagggccatg gtcaccagaa 60
gaagatgcta agcttaaaga attcattgaa aaatatggaa ctggtggtaa ttggattgct 120
cttcctctaa aagctggatt aaagagatgt ggaaagagct gcagattaag atggctaaat 180
tatctaaggc caaatataaa gcatggtgat ttttctgatg aagaagatag ggtaatatgc 240
agtttatatg ccagcattgg gagcaggtgg tcaattatag cagctcagtt accagggagg 300
accgacaatg atattaaaaa ctattggaac acaaagctta agaagaagct catgggtttt 360
attcagtcat catctaatat taaccagaga actaaatcac ctaatttatt attccctcct 420
acaagtactc ttcaaacaac ttttcaatcc caatctcaag catcaatttc aaatctttta 480
agagattcat atgtagagcc cattccacta gtccaaccaa atttcatgta caacaacaat 540
aacatgatga acttt 555
<210>229
<211>185
<212>PRT
<213>番茄
<400>229
Met Gly Arg Ala Pro Cys Cys Asp Lys Asn Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Phe Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Leu Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ala Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Phe Ile Gln Ser Ser Ser Asn Ile Asn
115 120 125
Gln Arg Thr Lys Ser Pro Asn Leu Leu Phe Pro Pro Thr Ser Thr Leu
130 135 140
Gln Thr Thr Phe Gln Ser Gln Ser Gln Ala Ser Ile Ser Asn Leu Leu
145 150 155 160
Arg Asp Ser Tyr Val Glu Pro Ile Pro Leu Val Gln Pro Asn Phe Met
165 170 175
Tyr Asn Asn Asn Asn Met Met Asn Phe
180 185
<210>230
<211>476
<212>DNA
<213>马铃薯
<400>230
atgggaagag ctccatgttg tgataaagca aatgtgaaga aagggccatg gtcacctgaa 60
gaagatgcaa aattaaaaga atatattaac aaatttggca ctggtggaaa ttggattgct 120
cttccacaaa aagctgggct aagaagatgt ggaaaaagct gcagattaag atggctaaat 180
tatcttaggc caaatattaa acacggagag ttttcagacg aagaagacag aatcatttgc 240
agcctttatg ctaacattgg aagcaggtgg tcaataatag cagctcaatt accaggcagg 300
acagataatg atatcaaaaa ctattggaac acaaagctca agaagaaatt aatgggattt 360
gtctcttcat ctcacaagat cattaggcct cttaatcatc atcattatca acaccaaatt 420
cccactaatt gttacaataa ttattcctca caaatttcac ttcttcaagc tccatc 476
<210>231
<211>159
<212>PRT
<213>马铃薯
<400>231
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Asn Lys Phe
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Arg
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ala Asn Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Phe Val Ser Ser Ser His Lys Ile Ile
115 120 125
Arg Pro Leu Asn His His His Tyr Gln His Gln Ile Pro Thr Asn Cys
130 135 140
Tyr Asn Asn Tyr Ser Ser Gln Ile Ser Leu Leu Gln Ala Pro Ser
145 150 155
<210>232
<211>471
<212>DNA
<213>高粱
<400>232
atggggagag ctccgtgctg cgacaaggct actgtgaaga agggtccgtg gtcgccggag 60
gaggacgcca agctcaagtc ctacatcgag cagaacggca ccggcggtaa ctggatagcc 120
ctgcctcaga agataggtct gaagaggtgt ggcaagagct gccgcctccg gtggctcaac 180
tacctccggc caaacatcaa gcacggtggg ttctccgagg aggaagacag aataatcctt 240
agcctctaca tcagcatagg cagcaggtgg tcgataatag cggcgcagct gccggggagg 300
acggacaatg acataaagaa ctactggaac acgaggctca agaagaagct cttcggcaag 360
caatcgcgca aggatcaacg gcagcatcag ttcatgcgcc agcaggcggc agcggcaaac 420
gatgggatga tgaagcaaga agcagcacaa cccgggatgc acccggaaca a 471
<210>233
<211>157
<212>PRT
<213>高粱
<400>233
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys Gln Ser Arg Lys Asp Gln Arg Gln
115 120 125
His Gln Phe Met Arg Gln Gln Ala Ala Ala Ala Asn Asp Gly Met Met
130 135 140
Lys Gln Glu Ala Ala Gln Pro Gly Met His Pro Glu Gln
145 150 155
<210>234
<211>609
<212>DNA
<213>高粱
<400>234
atgggacgga cgccgtgctg cgacagggcg gccgtgaagc ggggcccgtg gtcgccggag 60
gaggacgagg cgctgcggag ctacgtccag cgccacggca gcggcggcaa ctggatcgcc 120
atgcccaaga aagccgggct caagaggtgc ggcaagagct gcaggctgcg ctggctcaac 180
tacctccgcc ccgacatccg ccacggcggc ttcaccgccg aggaggacgc cgtcatcttg 240
tccctctaca cccagctcgg gagcaagtgg tcgctgatag cctcgcagat ggaggggagg 300
acggacaacg acgtcaagaa ccactggaac accaagctca agaagcgcct cctcgccgcc 360
gcgggcacct tctcgccggc accccacgcc cgggtgccgg cacccgcgcc tgctttccgt 420
accggacacg tttccccgtt gtcactggtc ccatcggttg gcataccgac cgtgaagaac 480
cgagcgtaca cctacgacga cttccttggc gccggcgctc gtgacccgtt cgccgccgcc 540
gacgggtcca cctcgggcgc cttccggggg tgtcggcctc caatggtcca cgggggacaa 600
cgggcccag 609
<210>235
<211>203
<212>PRT
<213>高粱
<400>235
Met Gly Arg Thr Pro Cys Cys Asp Arg Ala Ala Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Ala Leu Arg Ser Tyr Val Gln Arg His
20 25 30
Gly Ser Gly Gly Asn Trp Ile Ala Met Pro Lys Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Arg His Gly Gly Phe Thr Ala Glu Glu Asp Ala Val Ile Leu
65 70 75 80
Ser Leu Tyr Thr Gln Leu Gly Ser Lys Trp Ser Leu Ile Ala Ser Gln
85 90 95
Met Glu Gly Arg Thr Asp Asn Asp Val Lys Asn His Trp Asn Thr Lys
100 105 110
Leu Lys Lys Arg Leu Leu Ala Ala Ala Gly Thr Phe Ser Pro Ala Pro
115 120 125
His Ala Arg Val Pro Ala Pro Ala Pro Ala Phe Arg Thr Gly His Val
130 135 140
Ser Pro Leu Ser Leu Val Pro Ser Val Gly Ile Pro Thr Val Lys Asn
145 150 155 160
Arg Ala Tyr Thr Tyr Asp Asp Phe Leu Gly Ala Gly Ala Arg Asp Pro
165 170 175
Phe Ala Ala Ala Asp Gly Ser Thr Ser Gly Ala Phe Arg Gly Cys Arg
180 185 190
Pro Pro Met Val His Gly Gly Gln Arg Ala Gln
195 200
<210>236
<211>396
<212>DNA
<213>拟高粱
<400>236
atggggcgcg cgccgtgctg cgacaaggcg agcgtgaagc gggggccctg gtcgcccgag 60
gaggacgagc agctgcggag ctacgtccag cgcaacggca tcggcggcaa ctggatcgcc 120
ctgccgcaga aagcagggct gaaccggtgc ggcaagagct gccggctgcg gtggctcaac 180
tacctgcggc cggacatcaa gcacgggggc tacaccgagg aggaggaccg gatcatctgg 240
tcgctctaca gctccatcgg cagcaggtgg tccatcatcg cgtccaagct cccgggccgg 300
accgacaacg acgtcaagaa ctactggaac accaagctca agaagaaggc tatggccatg 360
gcggcggcgg ccggcctcag cgccagtagc agcgga 396
<210>237
<211>132
<212>PRT
<213>拟高粱
<400>237
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Glu Gln Leu Arg Ser Tyr Val Gln Arg Asn
20 25 30
Gly Ile Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Asn
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys His Gly Gly Tyr Thr Glu Glu Glu Asp Arg Ile Ile Trp
65 70 75 80
Ser Leu Tyr Ser Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ser Lys
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Val Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Ala Met Ala Met Ala Ala Ala Ala Gly Leu Ser Ala
115 120 125
Ser Ser Ser Gly
130
<210>238
<211>803
<212>DNA
<213>药蒲公英
<400>238
atggggagag caccttgctg tgataaagac aatgtaaaga aaggtccatg ggctcctgaa 60
gaagatgcta agcttaaagc atatattgaa gaacatggca ccggtggtaa ctggattgct 120
ttgcctcaga aaatcgggct caagagatgt ggaaagagtt gtcgcctccg gtggctaaat 180
tacctccggc caaatatcaa gcatggaggt ttctccgatg aagaagatcg cataatttgc 240
agcctctata ttagcatagg gagcaggtgg tctataattg cggcacaatt accggggcga 300
actgataacg atataaagaa ctactggaac acaaggctga agaagaagct cttgggtgca 360
ggtaaacaga gaaaagaaga aatatatgga agaaaacgag agttactaat gaaaaaagca 420
aggtcgacct catcggatat taatattcca tcatcgatag ttgtttccgg caaccaagac 480
ccagcttatt ggccggaaat gccgttgatg ccacctgtac cctactccaa taatcaagaa 540
ccgtgttttg ttaacgatca tgcttccata cgaaaactac tcatcaagct tggaggacga 600
tttactaatt ccgatcatga taatggaagt caatccacca aacacatcgt cgactctcac 660
tttccgatcg acactttcat tgatcttcag ggatcatcac aagaccatga tcagaagtta 720
attaatgcaa ttagtacgcc tctttcgtct tcttcatcat caatgttgct aaatagccca 780
tacaacatga tcatctttcc atc 803
<210>239
<211>268
<212>PRT
<213>药蒲公英
<400>239
Met Gly Arg Ala Pro Cys Cys Asp Lys Asp Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ala Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Asp Glu Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Ala Gly Lys Gln Arg Lys Glu Glu Ile
115 120 125
Tyr Gly Arg Lys Arg Glu Leu Leu Met Lys Lys Ala Arg Ser Thr Ser
130 135 140
Ser Asp Ile Asn Ile Pro Ser Ser Ile Val Val Ser Gly Asn Gln Asp
145 150 155 160
Pro Ala Tyr Trp Pro Glu Met Pro Leu Met Pro Pro Val Pro Tyr Ser
165 170 175
Asn Asn Gln Glu Pro Cys Phe Val Asn Asp His Ala Ser Ile Arg Lys
180 185 190
Leu Leu Ile Lys Leu Gly Gly Arg Phe Thr Asn Ser Asp His Asp Asn
195 200 205
Gly Ser Gln Ser Thr Lys His Ile Val Asp Ser His Phe Pro Ile Asp
210 215 220
Thr Phe Ile Asp Leu Gln Gly Ser Ser Gln Asp His Asp Gln Lys Leu
225 230 235 240
Ile Asn Ala Ile Ser Thr Pro Leu Ser Ser Ser Ser Ser Ser Met Leu
245 250 255
Leu Asn Ser Pro Tyr Asn Met Ile Ile Phe Pro Ser
260 265
<210>240
<211>632
<212>DNA
<213>Triphysaria pusilla
<400>240
atggggagag cgccttgttg tgacaaagcc aatgtaaaga gaggtccatg gtcacctgat 60
gaagacgatg ctcttaaatc ctacattcat aagcatggta ctggtggcaa ctggattgcc 120
ttgcctaaca aaattgggtt gaagagatgt ggaaagagtt gtcggctgag atggcttaat 180
tatctgcgcc caaacatcaa gcatggtggt tttactgaag aagaagataa ctttatttgc 240
aacctctata ttagtattgg aagcagatgg tctatcatcg cagctcaatt gcctggaaga 300
acagataacg atatcaaaaa ctactggaac actaggctaa ggaagaaact cttaggcagg 360
caacaaaggc gagaacaacg atccactaaa aacaatatta ttagagtggc cagttctgat 420
caaaatcatc aaaattacac attagcccat gaatattatt cttcaaattt cagtttatac 480
cctcagccgc cgccgccacc accggcacat atggtggcgc cttcctttat ccaggcggca 540
tcgaatccac aaaccaatca catttccttc atttcaccat tagtcgaaga accaacgaca 600
tttgcagctt gttatgatga tcaatcacaa gt 632
<210>241
<211>211
<212>PRT
<213>Triphysaria pusilla
<400>241
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Asp Ala Leu Lys Ser Tyr Ile His Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Asn Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Thr Glu Glu Glu Asp Asn Phe Ile Cys
65 70 75 80
Asn Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Arg Lys Lys Leu Leu Gly Arg Gln Gln Arg Arg Glu Gln Arg Ser
115 120 125
Thr Lys Asn Asn Ile Ile Arg Val Ala Ser Ser Asp Gln Asn His Gln
130 135 140
Asn Tyr Thr Leu Ala His Glu Tyr Tyr Ser Ser Asn Phe Ser Leu Tyr
145 150 155 160
Pro Gln Pro Pro Pro Pro Pro Pro Ala His Met Val Ala Pro Ser Phe
165 170 175
Ile Gln Ala Ala Ser Asn Pro Gln Thr Asn His Ile Ser Phe Ile Ser
180 185 190
Pro Leu Val Glu Glu Pro Thr Thr Phe Ala Ala Cys Tyr Asp Asp Gln
195 200 205
Ser Gln Val
210
<210>242
<211>651
<212>DNA
<213>Triphysaria pusilla
<400>242
atgggaagag caccgtgctg tgacaaagcc gatgtgaaga agggaccctg gtcaactgaa 60
gaagatgcaa cattgaaagc ttatatcgag aaatatggaa ctggtggaaa ttggattgcc 120
cttcctcaga aaatcgggct taagagatgt gggaagagtt gcagactgag gtggttgaat 180
tacttgaggc ctaatctcaa gcatggtggt tttactgatg aagaagataa tgttatttgc 240
agcctctata tcagcattgg cagcaggtgg tctataatag ctgcccagtt accaggaaga 300
acagataatg acatcgagaa ttactggaac accaggctca agaaaaaatt actcggcagg 360
cgcaaacaag ctaattataa cactaaccga ctctcggctt taggcggcca ggatgagcca 420
aaagaagatg cctataactt gcaaaaccta agcagttcag ccctcgaaag actccaactc 480
cacatgcatc ttcaaaccct tcaaaaccat aattcctcat tttattccaa taatccggca 540
atttggccca agatgatcga aaccctaaac tccttaaacg ataaacaaaa catgaataat 600
ctgatgcagc aaattatccc ctccacccct catcaactct gtccgcctgt a 651
<210>243
<211>217
<212>PRT
<213>Triphysaria pusilla
<400>243
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asp Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Thr Glu Glu Asp Ala Thr Leu Lys Ala Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Leu Lys His Gly Gly Phe Thr Asp Glu Glu Asp Asn Val Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Glu Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Arg Arg Lys Gln Ala Asn Tyr Asn Thr
115 120 125
Asn Arg Leu Ser Ala Leu Gly Gly Gln Asp Glu Pro Lys Glu Asp Ala
130 135 140
Tyr Asn Leu Gln Asn Leu Ser Ser Ser Ala Leu Glu Arg Leu Gln Leu
145 150 155 160
His Met His Leu Gln Thr Leu Gln Asn His Asn Ser Ser Phe Tyr Ser
165 170 175
Asn Asn Pro Ala Ile Trp Pro Lys Met Ile Glu Thr Leu Asn Ser Leu
180 185 190
Asn Asp Lys Gln Asn Met Asn Asn Leu Met Gln Gln Ile Ile Pro Ser
195 200 205
Thr Pro His Gln Leu Cys Pro Pro Val
210 215
<210>244
<211>636
<212>DNA
<213>Triphysaria pusilla
<220>
<221>其他特征
<222>(609)..(609)
<223>n是任意核苷
<400>244
atgggaagag caccttgctg tgacaaggca aaagtgaaga aagggccatg gtctcctgat 60
gaagattcta aactaaaaga atatatacaa aaacatggga ctattggaaa ttggattgct 120
ctcccacaca aagttggtct taaaagatgt ggcaaaagct gcagattgag atggctcaat 180
tatctgagac caaatattaa gcatggagat ttttcagatg atgaggatac aattatttgc 240
acacttttta atagcattgg aagcaggtgg tcagtaatag cagcacaatt accaggcaga 300
acagacaatg atatcaagaa ctactggaac acaaagctca aaaagaaact catggccaat 360
aatattttta tgcctccaat ttctacccat taccaaatga ggcctcacca agctaccatg 420
taccctacaa caccaaataa ctcctaccca ataccatatg aatatgccat tagcaattgt 480
tatactaatt cgaccccaat taaaatttat cagcccatat cccctaactt caccacgcta 540
ttttcttcaa actcgatcgg cccggatggg ttaatgggcc cggcccggcc tggatgggtt 600
acaaagatna tgaagaaatc ttccctgtgt tggtgg 636
<210>245
<211>212
<212>PRT
<213>Triphysaria pusilla
<220>
<221>其他特征
<222>(203)..(203)
<223>X是任意氨基酸
<400>245
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Lys Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ser Lys Leu Lys Glu Tyr Ile Gln Lys His
20 25 30
Gly Thr Ile Gly Asn Trp Ile Ala Leu Pro His Lys Val Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp Asp Glu Asp Thr Ile Ile Cys
65 70 75 80
Thr Leu Phe Asn Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Ala Asn Asn Ile Phe Met Pro Pro Ile Ser
115 120 125
Thr His Tyr Gln Met Arg Pro His Gln Ala Thr Met Tyr Pro Thr Thr
130 135 140
Pro Asn Asn Ser Tyr Pro Ile Pro Tyr Glu Tyr Ala Ile Ser Asn Cys
145 150 155 160
Tyr Thr Asn Ser Thr Pro Ile Lys Ile Tyr Gln Pro Ile Ser Pro Asn
165 170 175
Phe Thr Thr Leu Phe Ser Ser Asn Ser Ile Gly Pro Asp Gly Leu Met
180 185 190
Gly Pro Ala Arg Pro Gly Trp Val Thr Lys Xaa Met Lys Lys Ser Ser
195 200 205
Leu Cys Trp Trp
210
<210>246
<211>419
<212>DNA
<213>直果草属
<400>246
ggaagagcac cttgctgtga caaggcaaaa gtgaagaaag ggccatggtc tcctgatgaa 60
gattctaaac taaaggaata tatacaaaaa catgggacta ttggaaattg gattgctctc 120
ccacacaaag ttggtcttaa aagatgtggc aaaagctgca gattgagatg gctcaattat 180
ctgagaccaa atattaagca tggagatttt tcagatgatg aggatacaat tatttgcaca 240
ctttttaata gcattggaag caggtggtca gtaatagcag cacaattacc aggcagaaca 300
gacaatgata tcaagaacta ctggaacaca aagctcaaaa agaaactcat ggccaataat 360
atttttatgc ctccaatttc tacccattac caaatgaggc ctcaccaagc taccatgta 419
<210>247
<211>606
<212>DNA
<213>直果草属
<400>247
atgggaagag caccttgctg tgacaaggca aaagtgaaga aagggccatg gtctcctgat 60
gaagattcta aactaaaaga atatatacaa aaacatggga ctattggaaa ttggattgct 120
ctcccacaca aagttggtct taaaagatgt ggcaaaagct gcagattgag atggctcaat 180
tatctgagac caaatattaa gcatggagat ttttcagatg atgaggatac aattatttgc 240
acacttttta atagcattgg aagcaggtgg tcagtaatag cagcacaatt accaggcaga 300
acagacaatg atatcaagaa ctactggaac acaaagctca aaaagaaact catggccaat 360
aatattttta tgcctccaat ttctacccat taccaaatga ggcctcacca agctaccatg 420
taccatacaa caccaaataa ctcctaccca ataccatatg aatatgacat tagcaattgt 480
tatactaatt cgaccccaat taaaatttat cagcccatat cccctaactt caccacgcta 540
ttttcttcaa actcgatcgg cccggatggg ttaatgggcc cggcccggcc tggatgggtt 600
aacaaa 606
<210>248
<211>202
<212>PRT
<213>直果草属
<400>248
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Lys Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ser Lys Leu Lys Glu Tyr Ile Gln Lys His
20 25 30
Gly Thr Ile Gly Asn Trp Ile Ala Leu Pro His Lys Val Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Asp Asp Glu Asp Thr Ile Ile Cys
65 70 75 80
Thr Leu Phe Asn Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Ala Asn Asn Ile Phe Met Pro Pro Ile Ser
115 120 125
Thr His Tyr Gln Met Arg Pro His Gln Ala Thr Met Tyr His Thr Thr
130 135 140
Pro Asn Asn Ser Tyr Pro Ile Pro Tyr Glu Tyr Asp Ile Ser Asn Cys
145 150 155 160
Tyr Thr Asn Ser Thr Pro Ile Lys Ile Tyr Gln Pro Ile Ser Pro Asn
165 170 175
Phe Thr Thr Leu Phe Ser Ser Asn Ser Ile Gly Pro Asp Gly Leu Met
180 185 190
Gly Pro Ala Arg Pro Gly Trp Val Asn Lys
195 200
<210>249
<211>903
<212>DNA
<213>Tricticum aestivum
<400>249
atggggaggg cgccgtgctg cgacaaggcg acggtgaaga agggcccctg gtcgccggag 60
gaggacgcca agctcaaggc ctacatcgac gagaatggca ccggcggcaa ctggatcgcc 120
ctgccgcaga agatcgggct gaagaggtgc ggcaaaagct gtaggctcag atggctcaac 180
tatctgaggc caaacatcaa gcacggcgac ttcacagagg aagaggaaca catcatttgc 240
agcctctaca ttagcatcgg cagcaggtgg tcgatcatcg cggcgcagct gccgggcaga 300
acggacaacg acatcaagaa ctactggaac accaagctca agaagaagct cctcggcaag 360
cgcgcgccgt cccgccgcct gcagcgcgcc aaccaagacg cgccgatgcc ctactcctac 420
ctggcggcgg gcggcggcag cgccagcagc agcgggaacg ctagcggacg tcgtggaagc 480
cgagcaacag ctaaaccctt ccgctggagc gagctacgac atgccggtgc cgggcggttt 540
cgaggagcga tccgcctcca agctagggtt tggctctgct gccggcgagg ccgctggagt 600
ggccagtacc gtcgagatga gctcggcgtc aatggtaggc ggtggcttcg gctacggcca 660
cgtcgacgag ctgtacgact tcctctacag caagcagctt gccgcggccg gagcctttca 720
aggcggagtt cctcccctgc cggagctgca gtgcccggat ggcggcgccg tcgttggtgc 780
cgacgagaag ttctccacgt ggatggcgtc ttgcgatcac tatgttccta cgaccggcgg 840
ccagcagctc cagctccaag gtggaaactc aatgaatctg caggatttcg ttttagggta 900
tga 903
<210>250
<211>300
<212>PRT
<213>Tricticum aestivum
<400>250
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ala Tyr Ile Asp Glu Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Thr Glu Glu Glu Glu His Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Arg Ala Pro Ser Arg Arg Leu Gln
115 120 125
Arg Ala Asn Gln Asp Ala Pro Met Pro Tyr Ser Tyr Leu Ala Ala Gly
130 135 140
Gly Gly Ser Ala Ser Ser Ser Gly Asn Ala Ser Gly Arg Arg Gly Ser
145 150 155 160
Arg Ala Thr Ala Lys Pro Phe Arg Trp Ser Glu Leu Arg His Ala Gly
165 170 175
Ala Gly Arg Phe Arg Gly Ala Ile Arg Leu Gln Ala Arg Val Trp Leu
180 185 190
Cys Cys Arg Arg Gly Arg Trp Ser Gly Gln Tyr Arg Arg Asp Glu Leu
195 200 205
Gly Val Asn Gly Arg Arg Trp Leu Arg Leu Arg Pro Arg Arg Arg Ala
210 215 220
Val Arg Leu Pro Leu Gln Gln Ala Ala Cys Arg Gly Arg Ser Leu Ser
225 230 235 240
Arg Arg Ser Ser Ser Pro Ala Gly Ala Ala Val Pro Gly Trp Arg Arg
245 250 255
Arg Arg Trp Cys Arg Arg Glu Val Leu His Val Asp Gly Val Leu Arg
260 265 270
Ser Leu Cys Ser Tyr Asp Arg Arg Pro Ala Ala Pro Ala Pro Arg Trp
275 280 285
Lys Leu Asn Glu Ser Ala Gly Phe Arg Phe Arg Val
290 295 300
<210>251
<211>648
<212>DNA
<213>湿地越橘
<400>251
atgggaagag ctccatgttg tgacaaggca aatgtgaaga aagggccatg gtcccctgaa 60
gaagatgcaa agctaaaaga gtacatagat aagcatggga ctggagggaa ttggattgct 120
ctccctcaaa aagcagccct caaaagatgt ggaaaaagct gcagattgag atggctcaac 180
tatcttagac caaatatcaa acatggagag ttctctgatg atgaagatca taaaatctgc 240
agcctcttca ctactattgg aagcaggtgg tccataatag cagctcaatt gccaggaaga 300
acagataacg atatcaaaaa ctattggaac actaagctca agaagaaatt catgggaaaa 360
ttattccctc atccatctca aaatcagaga aaatcccacc aatccccaaa ttacccttca 420
tttttcccat tatattcacc acaatcccct acccaaacta gccatttcac aacctacgaa 480
aaccccattt cactcccacc aagttttttg aattccagtg ctaatttttc ctctgatgcc 540
actccaacaa ctattctaca agcccaagag agttatgtgg gtcccatgtt tggaggtgaa 600
gcaagttcca gttctgatgg gagttgcagc aaccaggtga tcagaggg 648
<210>252
<211>216
<212>PRT
<213>湿地越橘
<400>252
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Glu Tyr Ile Asp Lys His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Ala Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp His Lys Ile Cys
65 70 75 80
Ser Leu Phe Thr Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Phe Met Gly Lys Leu Phe Pro His Pro Ser Gln Asn
115 120 125
Gln Arg Lys Ser His Gln Ser Pro Asn Tyr Pro Ser Phe Phe Pro Leu
130 135 140
Tyr Ser Pro Gln Ser Pro Thr Gln Thr Ser His Phe Thr Thr Tyr Glu
145 150 155 160
Asn Pro Ile Ser Leu Pro Pro Ser Phe Leu Asn Ser Ser Ala Asn Phe
165 170 175
Ser Ser Asp Ala Thr Pro Thr Thr Ile Leu Gln Ala Gln Glu Ser Tyr
180 185 190
Val Gly Pro Met Phe Gly Gly Glu Ala Ser Ser Ser Ser Asp Gly Ser
195 200 205
Cys Ser Asn Gln Val Ile Arg Gly
210 215
<210>253
<211>1020
<212>DNA
<213>蒲桃
<400>253
atggggagag ctccttgctg tgacaaagcc aacgtcaaga aagggccatg gtcaccagag 60
gaagatgcca agctcaagac ttatattgag aaaaacggca ctgggggcaa ctggatagcc 120
ttacctcaaa agattggcct taagcgatgt gggaagagct gccgcctccg ctggttgaat 180
tatctcaggc ccaatatcaa acatggagga ttttcagagg aggaggataa catcatttgc 240
agcctctata taagtattgg aagcaggtgg tccgtcatcg cgactcaatt accgggaaga 300
actgataatg atattaagaa ctactggaac acaaggttga agaaaaagct actcggcaag 360
cagcggaaag aacaacaggc tcgccgagct aactacgtca agcaagagat gaagagagaa 420
cctgggattt ttgtggttcc tgatcaggcc gttggcagaa acccttattg ggcggagcta 480
cctgcagtat ctgtgcatcc gaaccaagat ccggatatca aggaccaagc atccattaag 540
aaattgctga tcaagctggg aggaagattt ggtgatgatg gtcaacaacc aggcagtaac 600
agcataaact ttcagtaccc tcttgatatt tccaccgcac aagatggtcc atatgagaca 660
tccatgaaca tgtttgcctc atctacgtcg accatgaatt caatcaacag tacgggttcc 720
caattgccaa acacccacta tgacgtcaat ggatcagctt caaatgttct tcaagggtat 780
aacagcctcc taaatgaact ggtgtatggc aacccacaac aattagatgg ctctgggagc 840
ttttacggaa tagaagacat cgccaatggt agcactggga ctagctccgc agaaagcagc 900
agttggggag atataaactc tctggtttac ccctctatca tctccaatta tggagttggc 960
caacaagggc cattacaaga ttctggtttt gaagagttga ggtacctggg ctcccaatag 1020
<210>254
<211>339
<212>PRT
<213>蒲桃
<400>254
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Thr Tyr Ile Glu Lys Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Thr Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Gln Ala Arg
115 120 125
Arg Ala Asn Tyr Val Lys Gln Glu Met Lys Arg Glu Pro Gly Ile Phe
130 135 140
Val Val Pro Asp Gln Ala Val Gly Arg Asn Pro Tyr Trp Ala Glu Leu
145 150 155 160
Pro Ala Val Ser Val His Pro Asn Gln Asp Pro Asp Ile Lys Asp Gln
165 170 175
Ala Ser Ile Lys Lys Leu Leu Ile Lys Leu Gly Gly Arg Phe Gly Asp
180 185 190
Asp Gly Gln Gln Pro Gly Ser Asn Ser Ile Asn Phe Gln Tyr Pro Leu
195 200 205
Asp Ile Ser Thr Ala Gln Asp Gly Pro Tyr Glu Thr Ser Met Asn Met
210 215 220
Phe Ala Ser Ser Thr Ser Thr Met Asn Ser Ile Asn Ser Thr Gly Ser
225 230 235 240
Gln Leu Pro Asn Thr His Tyr Asp Val Asn Gly Ser Ala Ser Asn Val
245 250 255
Leu Gln Gly Tyr Asn Ser Leu Leu Ash Glu Leu Val Tyr Gly Asn Pro
260 265 270
Gln Gln Leu Asp Gly Ser Gly Ser Phe Tyr Gly Ile Glu Asp Ile Ala
275 280 285
Asn Gly Ser Thr Gly Thr Ser Ser Ala Glu Ser Ser Ser Trp Gly Asp
290 295 300
Ile Asn Ser Leu Val Tyr Pro Ser Ile Ile Ser Asn Tyr Gly Val Gly
305 310 315 320
Gln Gln Gly Pro Leu Gln Asp Ser Gly Phe Glu Glu Leu Arg Tyr Leu
325 330 335
Gly Ser Gln
<210>255
<211>1101
<212>DNA
<213>蒲桃
<400>255
atgggtcgag ctccatgctg tgacaaagcc aacgtgaaga agggaccatg gtcccctgag 60
gaggatgcta agcttaaaac ttacattgag caatatggta ctggaggcaa ctggattgct 120
cttccccaga aaataggact taagagatgt ggaaaaagtt gcagactgag atggttgaat 180
tacttgagac ctaacatcaa gcatggtgga ttctctgaag aggaagacag catcatctgc 240
aacctctata taagtattgt tttttttttt ttgtggtcgg taattgcagc ccaattgcct 300
ggaagaacag ataatgacat taagaactac tggaacacaa gactgaagaa gaagttgctt 360
ggaaagcgta aacaatctca tttcaatcga ctttcggttg caggtcagga ccctaaagat 420
gcaactggtg tagaagataa tccatattca caggccttaa gcaattctgc ccttgaaaga 480
ctgcagctcc atatgcagct tcagagcctg caacacccct ttgctttcta caataatcct 540
gcactgtggc ccaagctgca tcccctccaa gaaaagctga tccagaacct ccagaatgag 600
ctccccagcc ctctgatgca acatacccca cctagttctg acccgatggg acaagaacag 660
aagtctgggt tctatgaacc gcccactgct tccatcacac ttcaacaaga gtatccgaaa 720
accaacaatc caaagggaga tgaatttgag aaatctttaa atgttttgcc ctcctcagac 780
agttcgatgg cctacaacag cgaaaatatg gtgggtgcac ctattatgtc taaaccagat 840
ggtgtagacc aatccaacat tgcacaacaa gcagtatcaa cctttcaagc tgagcttgat 900
gacttcctta ataacaaaat ggttggtttc ctcccacagg aagagcagat gactgaaata 960
gatggctcca aggacagctt ggcttggtgg tctaatgact ttgacacaaa atcggcatcc 1020
tcacactctt gggattccac ttctgttatt cagtctgagg ggatgttcca agattatgta 1080
ttaggttaca atctgcagtg a 1101
<210>256
<211>366
<212>PRT
<213>蒲桃
<400>256
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Thr Tyr Ile Glu Gln Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Gly Phe Ser Glu Glu Glu Asp Ser Ile Ile Cys
65 70 75 80
Asn Leu Tyr Ile Ser Ile Val Phe Phe Phe Leu Trp Ser Val Ile Ala
85 90 95
Ala Gln Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn
100 105 110
Thr Arg Leu Lys Lys Lys Leu Leu Gly Lys Arg Lys Gln Ser His Phe
115 120 125
Asn Arg Leu Ser Val Ala Gly Gln Asp Pro Lys Asp Ala Thr Gly Val
130 135 140
Glu Asp Asn Pro Tyr Ser Gln Ala Leu Ser Asn Ser Ala Leu Glu Arg
145 150 155 160
Leu Gln Leu His Met Gln Leu Gln Ser Leu Gln His Pro Phe Ala Phe
165 170 175
Tyr Asn Asn Pro Ala Leu Trp Pro Lys Leu His Pro Leu Gln Glu Lys
180 185 190
Leu Ile Gln Asn Leu Gln Asn Glu Leu Pro Ser Pro Leu Met Gln His
195 200 205
Thr Pro Pro Ser Ser Asp Pro Met Gly Gln Glu Gln Lys Ser Gly Phe
210 215 220
Tyr Glu Pro Pro Thr Ala Ser Ile Thr Leu Gln Gln Glu Tyr Pro Lys
225 230 235 240
Thr Asn Asn Pro Lys Gly Asp Glu Phe Glu Lys Ser Leu Asn Val Leu
245 250 255
Pro Ser Ser Asp Ser Ser Met Ala Tyr Asn Ser Glu Asn Met Val Gly
260 265 270
Ala Pro Ile Met Ser Lys Pro Asp Gly Val Asp Gln Ser Asn Ile Ala
275 280 285
Gln Gln Ala Val Ser Thr Phe Gln Ala Glu Leu Asp Asp Phe Leu Asn
290 295 300
Asn Lys Met Val Gly Phe Leu Pro Gln Glu Glu Gln Met Thr Glu Ile
305 310 315 320
Asp Gly Ser Lys Asp Ser Leu Ala Trp Trp Ser Asn Asp Phe Asp Thr
325 330 335
Lys Ser Ala Ser Ser His Ser Trp Asp Ser Thr Ser Val Ile Gln Ser
340 345 350
Glu Gly Met Phe Gln Asp Tyr Val Leu Gly Tyr Asn Leu Gln
355 360 365
<210>257
<211>1023
<212>DNA
<213>蒲桃
<400>257
atggggaggg caccttgctg tgacaaagcc aacgtcaaga aaggcccatg gtcgcctgaa 60
gaagatgcca agctcaagtc atatattgag cagcatggca ctggtggtaa ttggatcgct 120
ttgccacaaa aaattggcct caagagatgt gggaagagct gccgccttag atggttaaac 180
tatctccgcc caaatatcag gcacggagga ttctctgaag aagaagataa catcatctgt 240
agcctgtata taagtattgg aagcaggtgg tctgttattg cagcacaatt accaggacga 300
actgacaatg atataaagaa ctactggaat acgagactga agaagaagct ccttggtaag 360
cagcgcaaag aacaacaggc tcgcagaggt agctgcccga agcaagagat gaaaagagca 420
agtgggaacg ccatggtttc tgataatgaa aacttaaacc cttactggcc tgagttgccc 480
gtgccagcac caataccgta cccaaacgaa gaaccacgct tcaacgatca ttcatccatc 540
agaagattgt tgatcaggct tgggggaaga ttttctgatg acggacaacc actccaaaac 600
gggacaaatc ttcagctccc gattgatatt tcatccgttc aacaaccttt tgaccactca 660
gtaaactttc tgtcttcttc tcctatgaat gccctaaaca accctcgttc tgaatttcca 720
aacgctgagt acaatatgga agggggaggg ctacacatgc tacaaggaca gaacagtttt 780
ttagctgagc tggaggagat ggcttgtagc aacccacaaa gattagatgg gctggagttc 840
ttatatgcgc aggacatggc caataacaaa cccggatcaa ctgcttatga gcaaagtctc 900
agttggggtg agacgagctc tctggtttat cctcctgttg cttccaacta tgaaggtctt 960
caacagcaag gggtgacgca agaacatgac tttgatgagt tgaggtaccc taccccgcga 1020
taa 1023
<210>258
<211>340
<212>PRT
<213>蒲桃
<400>258
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Asn Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Val Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Arg
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Gln Arg Lys Glu Gln Gln Ala Arg
115 120 125
Arg Gly Ser Cys Pro Lys Gln Glu Met Lys Arg Ala Ser Gly Asn Ala
130 135 140
Met Val Ser Asp Asn Glu Asn Leu Asn Pro Tyr Trp Pro Glu Leu Pro
145 150 155 160
Val Pro Ala Pro Ile Pro Tyr Pro Asn Glu Glu Pro Arg Phe Asn Asp
165 170 175
His Ser Ser Ile Arg Arg Leu Leu Ile Arg Leu Gly Gly Arg Phe Ser
180 185 190
Asp Asp Gly Gln Pro Leu Gln Asn Gly Thr Asn Leu Gln Leu Pro Ile
195 200 205
Asp Ile Ser Ser Val Gln Gln Pro Phe Asp His Ser Val Asn Phe Leu
210 215 220
Ser Ser Ser Pro Met Asn Ala Leu Asn Asn Pro Arg Ser Glu Phe Pro
225 230 235 240
Asn Ala Glu Tyr Asn Met Glu Gly Gly Gly Leu His Met Leu Gln Gly
245 250 255
Gln Asn Ser Phe Leu Ala Glu Leu Glu Glu Met Ala Cys Ser Asn Pro
260 265 270
Gln Arg Leu Asp Gly Leu Glu Phe Leu Tyr Ala Gln Asp Met Ala Asn
275 280 285
Asn Lys Pro Gly Ser Thr Ala Tyr Glu Gln Ser Leu Ser Trp Gly Glu
290 295 300
Thr Ser Ser Leu Val Tyr Pro Pro Val Ala Ser Asn Tyr Glu Gly Leu
305 310 315 320
Gln Gln Gln Gly Val Thr Gln Glu His Asp Phe Asp Glu Leu Arg Tyr
325 330 335
Pro Thr Pro Arg
340
<210>259
<211>510
<212>DNA
<213>蒲桃
<400>259
atgggaagag ctccttgctg tgacaaggca aatgtgaaga aaggcccatg gtcacctgaa 60
gaagactcaa agcttaagga gtatattgag aaatatggga ctgggggaaa ttggattgct 120
ctcccacaaa aagctggtct taagagatgt gggaaaagct gcagattgag atggttgaac 180
tatctcagac ccaacattaa acatggagaa ttctctgatg atgaagatag gatcatctgc 240
agcgtctttg ctagcattgg aagcaagtgg tcagtaatag caaattactt gccggggagg 300
actgataacg atatcaagaa ctactggaac accaagctga agaagaaact catggggatg 360
gtgcctgtat ctcagagaaa accccatcaa gctaccttct ctcatcatct tcaaacctat 420
tcatcgttat catcaccttc acatgcggta acaacaacag caacttcatc atcatatgaa 480
agcaacagca gcaaccctta ttacactcca 510
<210>260
<211>170
<212>PRT
<213>蒲桃
<400>260
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ser Lys Leu Lys Glu Tyr Ile Glu Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Glu Phe Ser Asp Asp Glu Asp Arg Ile Ile Cys
65 70 75 80
Ser Val Phe Ala Ser Ile Gly Ser Lys Trp Ser Val Ile Ala Asn Tyr
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Met Val Pro Val Ser Gln Arg Lys Pro
115 120 125
His Gln Ala Thr Phe Ser His His Leu Gln Thr Tyr Ser Ser Leu Ser
130 135 140
Ser Pro Ser His Ala Val Thr Thr Thr Ala Thr Ser Ser Ser Tyr Glu
145 150 155 160
Ser Asn Ser Ser Asn Pro Tyr Tyr Thr Pro
165 170
<210>261
<211>944
<212>DNA
<213>玉蜀黍
<400>261
atggggagag ctccgtgctg cgacaaggct actgtgaaga agggcccgtg gtcaccggag 60
gaggacgcca agctcaagtc ctacatcgag cagaacggca ccggcggtaa ctggatagcc 120
ctgccgcaga agataggttt gaagaggtgc ggcaagagct gccgcctccg gtggctcaac 180
tacctccggc caaacatcag gcacggcggg ttctcggagg aggaggacag gataatcctt 240
agcctctaca tcagcatagg cagcaggtgg tcgataatag cggcgcagct gccggggagg 300
acggacaatg acataaagaa ctactggaac acgaagctca agaagaagct cttcggcaag 360
cagtcccgca gggatcagcg gcagcatcag ttcatgcgcc aggcagcggc ggcaagcgat 420
gggatgatga agcaagaagc ggcaaccggg gaggcagcgg gaagcagtag catggcggtg 480
gccaactaca actggcacca ccaccatcaa gccatggcgg ccgtgcctgt gcaaccaata 540
ctaggctcga taatggaagg ccatcgcgca ggggatgagg tagatgagtc gattcggaag 600
cttctttata agctaggagg agcaggccct tccacagcac tctcggttcc tcagtgtgtt 660
cctccaatgt actgtggaag tccagccctc atgccgccgc cgccgccgtc atgcccagca 720
gtggacaccg gcacatcgct tgatgaaggc ggcgtgcagg gctccggcgt gctgccggcg 780
ctggagatgg accatagctt ccacttcaac caggttaagc tagatggcct ggaaagcttc 840
ttcggcatga gtactgatca gagcatgcgg tggagtgagg tgagcccatt ggtttgccct 900
agcagtctgg ttgttgatga gccaggaaac cttgggatga agta 944
<210>262
<211>314
<212>PRT
<213>玉蜀黍
<400>262
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Thr Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Ser Tyr Ile Glu Gln Asn
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Arg His Gly Gly Phe Ser Glu Glu Glu Asp Arg Ile Ile Leu
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Phe Gly Lys Gln Ser Arg Arg Asp Gln Arg Gln
115 120 125
His Gln Phe Met Arg Gln Ala Ala Ala Ala Ser Asp Gly Met Met Lys
130 135 140
Gln Glu Ala Ala Thr Gly Glu Ala Ala Gly Ser Ser Ser Met Ala Val
145 150 155 160
Ala Asn Tyr Asn Trp His His His His Gln Ala Met Ala Ala Val Pro
165 170 175
Val Gln Pro Ile Leu Gly Ser Ile Met Glu Gly His Arg Ala Gly Asp
180 185 190
Glu Val Asp Glu Ser Ile Arg Lys Leu Leu Tyr Lys Leu Gly Gly Ala
195 200 205
Gly Pro Ser Thr Ala Leu Ser Val Pro Gln Cys Val Pro Pro Met Tyr
210 215 220
Cys Gly Ser Pro Ala Leu Met Pro Pro Pro Pro Pro Ser Cys Pro Ala
225 230 235 240
Val Asp Thr Gly Thr Ser Leu Asp Glu Gly Gly Val Gln Gly Ser Gly
245 250 255
Val Leu Pro Ala Leu Glu Met Asp His Ser Phe His Phe Asn Gln Val
260 265 270
Lys Leu Asp Gly Leu Glu Ser Phe Phe Gly Met Ser Thr Asp Gln Ser
275 280 285
Met Arg Trp Ser Glu Val Ser Pro Leu Val Cys Pro Ser Ser Leu Val
290 295 300
Val Asp Glu Pro Gly Asn Leu Gly Met Lys
305 310
<210>263
<211>1083
<212>DNA
<213>玉蜀黍
<400>263
atggggaggg cgccgtgctg cgacaaggcg agcgtgaaga aggggccgtg gtcgccggac 60
gaggacgcca agctcaaggc ctacatcgag gagcacggca ccggcggcaa ctggatcgcg 120
ctaccgcaca agattgggct gaagagatgc gggaaaagct gcaggctgag atggctcaat 180
tatctgaggc ccaatattaa gcatggtgac ttcacagaag aagaggagca tatcatttgc 240
agcctctaca ttagcattgg aagcaggtgg tccatcattg cagcgcagct gccggggcga 300
acggacaacg acatcaagaa ctactggaac accaagctga agaagaagct cctcggaaag 360
cgcgcgccgt ccaggcgcgc gaggacgaac caggatccct gctacctcgc cgcgggagcc 420
gccagcagca gcagcagcag cagcgccgcg acggccacgc aagccctaag cgcgtcggct 480
ctcgagcgga tccagctcca catgcggctc caaggcatct acggcgcgtt agcttgcagc 540
ggcggcaacg acgacagcaa cgctgccttc gggtcggcgg cggcggcgcc gccgcagtgg 600
ccgaagcttg aggcgctgtc gcaggccaac aggctgctcc cggggtcgct gccggcggac 660
gccatggcca caaccgtgag cgtgcagccg cacccacagc atctggtcga ggtcgaccac 720
caccagagcc tcgccgccgc cgcactcgag ggcgagcaac agctcagctc tgccggtgaa 780
ggaggctttt tcgagcggcc caaggtagat ttttactctc catcagccga ggtcgccgcc 840
gccgccgccg ccagcgtaga gatgaactcg gtcgccccga tggttgttgg cggctacgcg 900
ggcgccgccg gattcgggcc tcagcaccat cacgacgagc tctacgactt cctgtacagc 960
aagtacggat cagtgggcgg gctggcgcac gacggcgggc atgttccaac gttgccagag 1020
ctgcagtgcc cggacggcgc tgccgccgtc gtcggagccg acgagaagtt ctcggcgtgg 1080
acc 1083
<210>264
<211>361
<212>PRT
<213>玉蜀黍
<400>264
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Ser Val Lys Lys Gly Pro
1 5 10 15
Trp Ser Pro Asp Glu Asp Ala Lys Leu Lys Ala Tyr Ile Glu Glu His
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro His Lys Ile Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Thr Glu Glu Glu Glu His Ile Ile Cys
65 70 75 80
Ser Leu Tyr Ile Ser Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Leu Gly Lys Arg Ala Pro Ser Arg Arg Ala Arg
115 120 125
Thr Asn Gln Asp Pro Cys Tyr Leu Ala Ala Gly Ala Ala Ser Ser Ser
130 135 140
Ser Ser Ser Ser Ala Ala Thr Ala Thr Gln Ala Leu Ser Ala Ser Ala
145 150 155 160
Leu Glu Arg Ile Gln Leu His Met Arg Leu Gln Gly Ile Tyr Gly Ala
165 170 175
Leu Ala Cys Ser Gly Gly Asn Asp Asp Ser Asn Ala Ala Phe Gly Ser
180 185 190
Ala Ala Ala Ala Pro Pro Gln Trp Pro Lys Leu Glu Ala Leu Ser Gln
195 200 205
Ala Asn Arg Leu Leu Pro Gly Ser Leu Pro Ala Asp Ala Met Ala Thr
210 215 220
Thr Val Ser Val Gln Pro His Pro Gln His Leu Val Glu Val Asp His
225 230 235 240
His Gln Ser Leu Ala Ala Ala Ala Leu Glu Gly Glu Gln Gln Leu Ser
245 250 255
Ser Ala Gly Glu Gly Gly Phe Phe Glu Arg Pro Lys Val Asp Phe Tyr
260 265 270
Ser Pro Ser Ala Glu Val Ala Ala Ala Ala Ala Ala Ser Val Glu Met
275 280 285
Asn Ser Val Ala Pro Met Val Val Gly Gly Tyr Ala Gly Ala Ala Gly
290 295 300
Phe Gly Pro Gln His His His Asp Glu Leu Tyr Asp Phe Leu Tyr Ser
305 310 315 320
Lys Tyr Gly Ser Val Gly Gly Leu Ala His Asp Gly Gly His Val Pro
325 330 335
Thr Leu Pro Glu Leu Gln Cys Pro Asp Gly Ala Ala Ala Val Val Gly
340 345 350
Ala Asp Glu Lys Phe Ser Ala Trp Thr
355 360
<210>265
<211>1259
<212>DNA
<213>水稻
<400>265
atggggagag cgccgtgctg cgacaaggcg agcgtgaaga aggggccatg gtcaccggag 60
gaggacgcga agctcaaatc ctacatcgag cagaacggca ccggcggcaa ctggatcgcc 120
ctgccccaga agatcggtat gtgttggcaa ttctcatgag tcatgagtcg atgtggtggt 180
tgtggcagca gcagcagcga tagtggtaga agaaagctaa tcaagtgcat gtgtgtcttt 240
gttggtttgg gtttgtacat gtaggtttga aaaggtgcgg caagagctgc cgcctccggt 300
ggctgaacta cctccggccg aacatcaagc acggcgggtt ctcggaggag gaagacagga 360
tcatcctcag cctctacatc agcataggaa gcaggtatac ttgctaattc agctgggcac 420
acacaagtct agtcactcct atgatcaact caactgtaga attagctgcg aaaccggtag 480
gcaggcaggc aggttgcaca tggggcatga gagtgtagta caatctatga tctgacatga 540
ccatatatgc atggcacagg tggtcgataa tagcagcgca gctgccgggg cggacggaca 600
atgacatcaa gaactattgg aacacgaggc tcaagaagaa gctctttggc aagcagtcgc 660
gcaaggatca gaggcagcag cagcacctgg cgcgccaggc ggcagcagct gccagcgact 720
tgcagatcaa acaagaagcg agcaggggtg caaacgaagc cgatggcttg gctgccggtg 780
ccaattacac ttggcatcac caccacgcca tggccgtgcc tgtgcacccg atgtcggcac 840
caatggtggt ggaaggaggc cgtgtgggag acgatgtcga tgagtcgatc cggaagcttc 900
tgttcaagct cggagggaac ccattcgcgg cctcgccggc accgccatgc atacctccac 960
caccaatgta cgaggaagcc ccaagcttcg tgccaccatt ggcgcacggc gtgccgctca 1020
acgaaggcgg catgcagtgc tccagcgtgc tgccggcgct ggagctggac gagaacttcc 1080
acttcaacca tgtcaagctg gacgggctcg agtgcctctt cgggatggga gatcaccaaa 1140
acatgagatg gaatgaggtg agcccgttgg tttgccctaa taacgctgtg gcgtccagct 1200
cccaagggat gcagcagtac tgcctagttg aagaaccagc tgacctcggg atgcagtag 1259
<210>266
<211>116
<212>PRT
<213>拟南芥
<220>
<221>其他特征
<222>(4)..(4)
<223>4位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(9)..(9)
<223>9位的X可以是赖氨酸或者精氨酸
<220>
<221>其他特征
<222>(10)..(11)
<223>10和11位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(12)..(12)
<223>12位的X可以是甲硫氨酸或者缬氨酸
<220>
<221>其他特征
<222>(14)..(14)
<223>14位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(16)..(16)
<223>16位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(18)..(18)
<223>18位的X可以是丝氨酸或者丙氨酸
<220>
<221>其他特征
<222>(19)..(19)
<223>19位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(20)..(20)
<223>20位的X可以是天门冬氨酸、谷氨酸盐或者谷氨酸
<220>
<221>其他特征
<222>(23)..(24)
<223>23和24位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(25)..(25)
<223>25位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(26)..(26)
<223>26位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(27)..(27)
<223>27位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(28)..(28)
<223>28位的X可以是苯丙氨酸或者酪氨酸
<220>
<221>其他特征
<222>(29)..(32)
<223>29-32位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(34)..(36)
<223>34-36位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(37)..(37)
<223>37位的X可以是丝氨酸或者天门冬氨酸
<220>
<221>其他特征
<222>(40)..(41)
<223>40-41位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(43)..(43)
<223>43位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(44)..(44)
<223>44位的X可以是精氨酸或者赖氨酸精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(45)..(45)
<223>45位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(47)..(47)
<223>47位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(48)..(48)
<223>48位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(52)..(52)
<223>52位的X可以是赖氨酸或者精氨酸
<220>
<221>其他特征
<222>(59)..(59)
<223>59位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(65)..(65)
<223>65位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(66)..(66)
<223>66位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(67)..(67)
<223>67位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(70)..(70)
<223>70位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(71)..(71)
<223>71位的X可以是苯丙氨酸或者酪氨酸
<220>
<221>其他特征
<222>(72)..(72)
<223>72位的X可以是丝氨酸或者苏氨酸
<220>
<221>其他特征
<222>(73)..(74)
<223>73-74位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(76)..(76)
<223>76位的X可以是天门冬氨酸或者谷氨酸
<220>
<221>其他特征
<222>(77)..(78)
<223>77-78位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(79)..(79)
<223>79位的X可以是异亮氨酸或者缬氨酸
<220>
<221>其他特征
<222>(80)..(82)
<223>80-82位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(83)..(83)
<223>83位的X可以是苯丙氨酸或者酪氨酸
<220>
<221>其他特征
<222>(84)..(86)
<223>84-86位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(89)..(89)
<223>89位的X可以是赖氨酸或者精氨酸
<220>
<221>其他特征
<222>(92)..(92)
<223>92位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(93)..(93)
<223>93位的X可以是甲硫氨酸或者异亮氨酸
<220>
<221>其他特征
<222>(95)..(96)
<223>95-96位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(97)..(97)
<223>97位的X可以是甲硫氨酸或者亮氨酸
<220>
<221>其他特征
<222>(98)..(99)
<223>98-99位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(105)..(105)
<223>105位的X可以是异亮氨酸,亮氨酸或者缬氨酸
<220>
<221>其他特征
<222>(108)..(108)
<223>108位的X可以是组氨酸或者酪氨酸
<220>
<221>其他特征
<222>(110)..(110)
<223>110位的X可以是天门冬氨酸或者天门冬氨酸
<220>
<221>其他特征
<222>(111)..(111)
<223>111位的X可以是丝氨酸或者苏氨酸
<220>
<221>其他特征
<222>(112)..(112)
<223>112位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(114)..(114)
<223>114位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(115)..(115)
<223>115位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(116)..(116)
<223>116位的X可以是精氨酸或者赖氨酸
<400>266
Met Gly Arg Xaa Pro Cys Cys Asp Xaa Xaa Xaa Xaa Lys Xaa Gly Xaa
1 5 10 15
Trp Xaa Xaa Xaa Glu Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
20 25 30
Gly Xaa Xaa Xaa Xaa Trp Ile Xaa Xaa Pro Xaa Xaa Xaa Gly Xaa Xaa
35 40 45
Arg Cys Gly Xaa Ser Cys Arg Leu Arg Trp Xaa Asn Tyr Leu Arg Pro
50 55 60
Xaa Xaa Xaa His Gly Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Xaa
65 70 75 80
Xaa Xaa Xaa Xaa Xaa Xaa Gly Ser Xaa Trp Ser Xaa Xaa Ala Xaa Xaa
85 90 95
Xaa Xaa Xaa Arg Thr Asp Asn Asp Xaa Lys Asn Xaa Trp Xaa Xaa Xaa
100 105 110
Leu Xaa Xaa Xaa
115
<210>267
<211>87
<212>PRT
<213>拟南芥
<220>
<221>其他特征
<222>(1)..(1)
<223>1位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(2)..(10)
<223>2-10位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(12)..(13)
<223>12-13位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(15)..(42)
<223>15-42位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(44)..(57)
<223>44-57位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(58)..(58)
<223>58位的X可以是酪氨酸或者苯丙氨酸
<220>
<221>其他特征
<222>(59)..(62)
<223>59-62位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(64)..(86)
<223>64-86位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(87)..(87)
<223>87位的X可以是精氨酸或者赖氨酸
<400>267
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Ile Xaa Xaa
1 5 10 15
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
20 25 30
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa
35 40 45
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa
50 55 60
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
65 70 75 80
Xaa Xaa Xaa Xaa Xaa Xaa Xaa
85
<210>268
<211>87
<212>PRT
<213>拟南芥
<220>
<221>其他特征
<22>(2)..(2)
<223>2位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(4)..(6)
<223>4-6位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(9)..(10)
<223>9-10位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(11)..(11)
<223>11位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(12)..(12)
<223>12位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(13)..(18)
<223>13-18位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(20)..(42)
<223>20-42位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(45)..(45)
<223>45位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(51)..(51)
<223>51位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(52)..(52)
<223>52位的X可以是异亮氨酸或者亮氨酸
<220>
<221>其他特征
<222>(53)..(53)
<223>53位的X可以是精氨酸或者赖氨酸
<220>
<221>其他特征
<222>(56)..(56)
<223>56位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(57)..(57)
<223>57位的X可以是苯丙氨酸或者酪氨酸
<220>
<221>其他特征
<222>(58)..(60)
<223>58-60位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(62)..(62)
<223>62位的X可以是天门冬氨酸或者谷氨酸
<220>
<221>其他特征
<222>(63)..(75)
<223>63-75位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(77)..(79)
<223>77-79位的X可以是任意氨基酸
<220>
<221>其他特征
<222>(81)..(85)
<223>81-85位的X可以是任意氨基酸
<400>268
Gly Xaa Trp Xaa Xaa Xaa Glu Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
1 5 10 15
Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa
20 25 30
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Trp Xaa Asn Tyr Leu
35 40 45
Arg Pro Xaa Xaa Xaa His Gly Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa Xaa
50 55 60
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Xaa Xaa Ala
65 70 75 80
Xaa Xaa Xaa Xaa Xaa Arg Thr
85
<210>269
<211>10
<212>PRT
<213>拟南芥
<400>269
Ser Phe Ser Gln Leu Leu Leu Asp Pro Asn
1 5 10
<210>270
<211>15
<212>PRT
<213>拟南芥
<400>270
Thr Ser Thr Ser Ala Asp Gln Ser Thr Ile Ser Trp Glu Asp Ile
1 5 10 15
<210>271
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>271
acgttctaga atgggaagag caccgtgttg 30
<210>272
<211>32
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>272
atcgggatcc ttacacatga tttggcgcat tg 32
<210>273
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>273
acgttctaga atgggaagag caccgtgttg 30
<210>274
<211>37
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>274
atcgggatcc ttaaaaaaat tgctttgaat cagaata 37
<210>275
<211>27
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>275
acgtaagctt tcgtaaaatc tctcatg 27
<210>276
<211>40
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>276
gtcactcgag cctaggtttc ttgattcttg attcttgatc 40
<210>277
<211>35
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>277
aaagtcgacg catctttaca atgtaaagct tttct 35
<210>278
<211>27
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>278
aaatctagat gttcgttgct tttcggg 27
<210>279
<211>38
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>279
aaagtcgaca gaagacaaat gagagttggt ttatattt 38
<210>280
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>280
aaatctagac gcaacgaact ttgattcaa 29
<210>281
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>281
atgggaagag caccgtgttg tgataaggcc 30
<210>282
<211>35
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>282
ttaatttggc gcattgaagt aacttgcatc ttcgg 35
<210>283
<211>28
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>283
acgttctaga atggggagag cgccgtgc 28
<210>284
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>284
tgcaggatcc tactgcatcc cgaggtcag 29
<210>285
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>285
acgttctaga atggggagag ctccttgttg 30
<210>286
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>286
acgtggatcc ctattgcgct cctcctggg 29
<210>287
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>287
acgttctaga atggggaggg caccttgct 29
<210>288
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>288
acgttctaga atggggagag ctccgtgct 29
<210>289
<211>28
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>289
actgtctaga atggggaggg cgccgtgc 28
<210>290
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>290
actgtctaga atgggaagag ctccttgctg t 31
<210>291
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>291
acgttctaga atggggagag ctccgtgct 29
<210>292
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>292
actgtctaga atgggaagag ctccatgttg t 31
<210>293
<211>35
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>293
gactggatcc ttagtaataa aacatcccta tctca 35
<210>294
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>294
acgttctaga atggggagag ctccttgctg 30
<210>295
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>295
gactggatcc tcattgtggc ccaaagaagc t 31
<210>296
<211>35
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>296
aaagtcgacg catctttaca atgtaaagct tttct 35
<210>297
<211>27
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>297
aaatctagat gttcgttgct tttcggg 27
<210>298
<211>38
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>298
aaagtcgaca gaagacaaat gagagttggt ttatattt 38
<210>299
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>299
aaatctagac gcaacgaact ttgattcaa 29
<210>300
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>300
aaaggatcca tgggaagagc accgtgttg 29
<210>301
<211>32
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>301
aaaggatccc cactccctaa agacacagat tt 32
<210>302
<211>28
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>302
aaatctagaa tgggaagagc accgtgtt 28
<210>303
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>303
aaaggatcct tacacatgat ttggcgcat 29
<210>304
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>304
actgtctaga atgggaagag ctccatgctg 30
<210>305
<211>32
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>305
cagtggatcc ttaaacactg tggtagctca tc 32
<210>306
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>306
actgtctaga atgggaagag ctccgtgttg 30
<210>307
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>307
acgtggatcc taggagtaga aatagggcaa g 31
<210>308
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>308
actgtctaga atgggtaggg ctccatgttg t 31
<210>309
<211>34
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>309
acgtggatcc tcagtagtac aacatgaact tatc 34
<210>310
<211>28
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>310
aaaatctaga atgggaagag caccgtgc 28
<210>311
<211>31
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>311
aaaaagatct ctactcatta tcgtatagag g 31
<210>312
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>312
acgtgtcgac gaagcagcag aagccttgat 30
<210>313
<211>32
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>313
acgttctaga ggtagagaaa agagaaagcc tc 32
<210>314
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>314
acgttctaga atggggagag cgccgtgctg 30
<210>315
<211>32
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>315
tgcaggatcc ctactgcatc ccgaggtcag ct 32
<210>316
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>316
acgttctaga atggggaggg caccttgct 29
<210>317
<211>28
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>317
acgtggatcc tattgcgccc ccgggtag 28
<210>318
<211>34
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>318
aaatctagaa tggggagagc tccgtgctgc gaca 34
<210>319
<211>34
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>319
aaaggatccc tacttcatcc caaggtttcc tggc 34
<210>320
<211>30
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>320
acgttctaga atggggagag ctccttgttg 30
<210>321
<211>29
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>321
acgtggatcc ctattgcgct cctcctggg 29
<210>322
<211>39
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>322
aaatc tagaa tgggaagagc accgtgttgt gataaggcc 39
<210>323
<211>42
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>323
aaaggatcct tacacattat ttggcccatt gaagtatctt gc 42
<210>324
<211>39
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>324
aaatctagaa tgggaagagc accgtgttgt gacaaggct 39
<210>325
<211>42
<212>DNA
<213>人工序列
<220>
<223>合成引物
<400>325
aaaggatcct tacaaatgat ttgccccatt gaagtaactt gc 42

Claims (6)

1.MYB亚群-14多肽在热胁迫条件下与野生型植物相比降低种子产量损失的用途,其中所述MYB亚群-14包括至少下述的成员:MYB68,MYB36,MYB84,MYB37,MYB38以及MYB87,并且其中所述用途通过包括以下步骤的方法实现:
a)提供一种核酸,所述的核酸能够编码MYB亚群-14多肽;
b)将所述的核酸插入到一个载体之中;
c)利用所述的载体对一种植物、组织培养物、或者一种植物细胞进行转化,从而获得一种与野生型植物相比具有增加的所述Myb亚群-14多肽的表达的转基因植物、组织培养物或者植物细胞;
d)使所述的转基因植物进行生长或者从所述的转基因组织培养物或者植物细胞中进行一种植物的再生,其中生成了一种具有热胁迫耐受性的植物,与野生型植物相比,在热胁迫条件下具有热胁迫耐受性的植物具有增加的热胁迫耐受性和降低的种子产量损失。
2.根据权利要求1所述的用途,其中,所述MYB亚群-14多肽选自由MYB36、MYB37和MYB68所组成的组中。
3.根据权利要求1所述的用途,其中,所述MYB亚群-14多肽是MYB68。
4.根据权利要求1所述的用途,其中,所述载体包括组成型启动子或者诱导型启动子。
5.根据权利要求1所述的用途,其中,所得转基因植物与野生型植物相比具有增加的结实产量。
6.根据权利要求1所述的用途,其中,所得热胁迫耐受性的植物具有降低的花朵败育。
CN200880020353.0A 2007-04-18 2008-04-18 对于热胁迫具有增强的耐受力的植物 Active CN101981192B (zh)

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WO2009027824A8 (en) 2009-06-11
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