CN101963613B - Detection means - Google Patents

Abstract

The present invention provides a detection means, and the device includes：One detecting element (102), it includes the sample areas (1021) of contact liq sample and the detection zone (1022) of a liquid sample of the reception from sample areas；With the reading equipment for being used to read testing result in detection zone, the equipment includes electronic component；The detection means also includes one and obstructs the liquid sample contact electronic component contacted with sample areas or the barrier component being leaked to outside detection means.It unexpectedly be contacted electronic component by can will not be allowed liquid sample when using the equipment or be leaked to the outside of detection means using the device, while improving the stability and processing safety of detection means.

Description

Detection means

Technical field

The invention belongs to diagnose detection field, particularly belong to the field of analyte in detection sample, especially belong to inspection In test sample sheet whether the Rapid diagnostic detection device in terms of the metabolin containing drugs of abuse or forepart pregnant women.

Background technology

In recent years, detection means is widely used analyzed in the body fluid of people, such as urine, saliva or blood to detect The indicator substance of material, such as drug abuse or disease.So traditional detection means usually requires to collect liquid sample to one In individual container, then then taking out reading again (visually sees or read with machine) detecting element in detecting element insertion liquid On test result.So it is possible to allow operator to be contaminated by the sample.Using this detection means except by professional testing staff Using outer, gradually there is no the people of professional experiences to use by some, for example, use in the family, this is accomplished by providing a kind of operation more Simply, testing result more accurately detection means.The device of test result is directly read using electronic technology and is examined because obtaining Result is surveyed directly, simply to become more popular.But, when test result reading is carried out with these electronic equipments, by In incorrect operation detected liquid sample may be allowed to flow on electronic equipment, so as to damage the electricity in these equipment Subcomponent causes incorrect reading, or even can not complete detection；In addition, working as needs to make further the sample in detection means When confirming chemical examination, it is often necessary to whole detection means is transported another place and chemically examined, in transportation The leakage of liquid sample may also be caused.This is accomplished by one safer detection means of offer and meets these needs.

The content of the invention

In order to solve problem above, the present invention provides a kind of detection means, including：One detecting element, it includes contact The sample areas of liquid sample and a detection zone of the reception from sample areas liquid sample；It is used to read detection with one The reading equipment of testing result on region, the equipment includes electronic component；Wherein, the detection means also includes a barrier and sample The liquid sample contact electronic component of one's respective area contact or the barrier component being leaked to outside detection means.Can be with using the device Liquid sample will not be allowed unexpectedly to contact electronic component or the outside of detection means is leaked to when using the equipment, simultaneously Improve the stability and processing safety of detection means.

In some more preferably embodiment, barrier component be located at detecting element on sample areas and electronic component it Between.Device can also include a test chamber, and test chamber is divided into detection epicoele and detection cavity of resorption, described portion by barrier component Sample areas is divided to be located at the lower intracavitary of detection, electronic unit, which is located at, detects upper intracavitary.More excellent, a ring is also included on barrier component Shape cell body, sets one to be used for the elastomeric element of hermetic barrier part and detection luminal wall surface on ring-type cell body.Preferably, Barrier component, which also includes one, to be used to absorb the absorber element for carrying out Autonomous test cavity of resorption liquid sample.More excellent, absorber element is located at In one housing.In addition, absorber element can also be located at the upper intracavitary of detection.

Other preferred embodiment in, barrier component also includes through hole, and the sample areas of detecting element is led to The through hole is crossed to be located in detection cavity of resorption.In some preferred modes, housing can be connected as a single entity with barrier component.Detection means A lid can also be included, test chamber and lid are connected as a single entity.

In other specific embodiment, the device also includes a liquid sample transfer chamber, wherein in transfer One aperture film is set between chamber and test chamber；Wherein, when the detection means is inserted into a liquid sample collection chamber, Liquid sample in collecting chamber enters in liquid sample transfer chamber, but the liquid sample can not enter detection by described film In chamber；And, wherein, when a piston is inserted into liquid sample transfer chamber, the piston forces the partially liq in transfer chamber Sample is entered in test chamber by the porous membrane.More excellent, aperture is arranged to surface tension of liquid, when described turn When the pressure differential of shifting chamber and test chamber is less than or equal to the surface tension of liquid set by the aperture, the liquid in transfer chamber Sample can not enter test chamber by the aperture；When the pressure differential of the transfer chamber and test chamber is more than the liquid set by the aperture When body surface tension, the liquid in transfer chamber can be entered in test chamber by described aperture.More preferably, lid, inspection Chamber is surveyed, transfer chamber and porous membrane are structure as a whole, and can be plastic mould single injection-molded.

In other embodiment, described reading equipment includes：Counting circuit, response represents a kind of chemical examination The signal of the amount of thing or the accumulative speed of assay, if the signal chemical examination do not complete before exceed default first threshold or Less than default Second Threshold, then it represents that result of laboratory test, wherein, described Second Threshold is less than first threshold.In addition, described Reading equipment can also include：At least one light source, it can light, and these light incide on the detecting element two or many In at least one in the individual area being spatially separated；At least one photodetector, is arranged it is positioned such that can detect from two The light of each area outflow in the individual area, and produce the signal represented in respective area presence or absence of liquid sample；With And a counting circuit, the signal is responded to calculate liquid sample along the stream flowed between the detecting element Shang Liangge areas Speed；Judge calculated flow velocity whether outside acceptable predetermined limit.Reading equipment can also include：First, second and Three light sources, it can send is spatially separated from for irradiating corresponding first, second, and third on the detecting element The light in region；With the first and second photodetectors, wherein first photodetector is used to detect from first and secondth area Each light sent and second photodetector in domain are used to detecting each in region from described second and the 3rd The individual light sent.It is used to judge the whether enough electrodes of liquid sample in addition, reading and can also include one in equipment.

Beneficial effect

The present invention settles detection sample at one go, liquid sample will not be allowed unexpectedly to contact electricity when using the equipment Subcomponent or the outside for being leaked to detection means, while improving the stability and processing safety of detection means.

Brief description of the drawings

Fig. 1 is some specific embodiment dimensional structure diagrams of the device of the present invention；

Fig. 2 is the structural blast schematic diagram of Fig. 1 shown devices；

Fig. 3 is the dimensional structure diagram of collection device；

Fig. 4 is the assembling vertical section schematic diagram of Fig. 1 shown devices；

Fig. 5 is the assembling cross-sectional of Fig. 1 shown devices；

Fig. 6 is the structural blast schematic diagram of detection means in a specific example of the invention；

Fig. 7 is the assembling dimensional structure diagram of the detection means shown in Fig. 6；

Fig. 8 is the vertical section schematic perspective view and part A enlarged diagram of the detection means shown in Fig. 6；

Fig. 9 is the cross section schematic perspective view and part B enlarged diagram of the detection means shown in Fig. 6.

Description of reference numerals：

Detection device component 10, detection means 101, test chamber 14, transfer chamber 15, lid 19, collection device 72, collecting chamber 721, collecting chamber opening 723, piston 722, collecting chamber bottom 724, test chamber opening 142, test chamber interior walls 141, in detection Chamber 143, detects cavity of resorption 144, plastic tab 16, elastic sealing ring 13, transfer chamber opening 204, detecting element 102, detecting element Sample areas 1021, the detection zone 1022 of detecting element, absorber element 11, circuit board 103, tabula disconnected 109, lower wall 129, downside wall opening 120, lower wall through hole 124, ring-type cell body 131, detecting element supporting construction 123,122, left side wall 128, right side wall 127, housing 12, bottom plate 125, pin hole 121, latch 1211, display screen 1011, bayonet arrangement 119, electrode 107, hemispherical barrier element 108, attachment structure 104, slit 456.

Embodiment

In the following detailed description, accompanying drawing and corresponding explanatory note only illustrate that the present invention can by way of example The mode of actable specific concrete scheme.We are not precluded from the present invention can also be without prejudice to scope of the invention as claimed Any other interior embodiment.

The present invention provides a kind of detection means.This device includes：One detecting element 102, it includes contact liq sample The detection zone 1022 of this sample areas 1021 and a liquid sample of the reception from sample areas；It is used to read with one The reading equipment of testing result in detection zone, the equipment includes electronic component；The detection means also includes a barrier and sample The liquid sample contact electronic component of one's respective area contact or the barrier component being leaked to outside detection means.

In a specific example, read as shown in fig. 6, a barrier component part is located in equipment, another part can With individualism, the two parts, which cooperate, constitutes complete barrier component.For example, positioned at the partial obstruction read in equipment Part can be tabula disconnected 109, and another part barrier component can be a cut-off 129 for carrying opening 120, tabula disconnected 109 Cooperation is coordinated to reach hydraulic seal with the form 119 of the bayonet socket of opening 124.The mode of cooperation has a lot, for example, allow tabula disconnected and big Small and shape is corresponding with opening 120.More excellent, a through hole 124, the size and detecting element of through hole are included on barrier component Cross section matching, the sample areas of detecting element passes through through hole 124, and detecting element is divided into two portions by such barrier component Point, one end of sample areas is used for contact liq sample, and one end of detection zone is used for being read equipment reading testing result simultaneously It is shown on display screen 1011.When using the detection means, liquid sample region and liquid sample on detecting element When contact, due to there is such barrier component, contact with can so avoiding liquid sample " accident " in reading equipment Electronic component, so as to damage reading equipment." accident " mentioned here refers in any operating process or detection means is complete During after into detection, because artificial or objective extraneous factor causes liquid sample to contact the electricity read in equipment Subcomponent, or any situation or the condition being leaked to outside detection means.Detection means is inserted into liquid sample for example, working as In allow detecting element sample areas contact liq sample when, due to liquid is shaken and may be splashed to reading equipment on Electronic component, so as to damage electronic component；This rock may be due to unconsciously quick insertion detecting element to liquid-like In this or operator deliberately causes and whole detection can not be gone on.

Reading equipment includes the circuit board 103 containing electronic component and coordinates to examine on electronic component reading detecting element 102 Survey the attachment structure 104 of test result on region 1022.The electronic component read in equipment can include：Counting circuit, response The signal of a kind of amount of assay or the accumulative speed of assay is represented, is preset if the signal exceedes before chemical examination is not completed First threshold or less than default Second Threshold, then it represents that result of laboratory test, wherein, described Second Threshold is less than the first threshold Value.At least one light source can also be included by reading the electronic component in equipment, and it can light, and these light incide the detection In at least one on element in two or more areas being spatially separated；At least one photodetector, arranges that its position makes The light from each area outflow in two areas can be detected by obtaining, and produce expression in respective area presence or absence of liquid The signal of sample；And a counting circuit, the signal is responded to calculate liquid sample along the detecting element Shang Liangge areas Between the flow velocity that flows；Judge calculated flow velocity whether outside acceptable predetermined limit.Read the electronic component in equipment It can also include：First, second, and third light source, its can send for irradiate on the detecting element corresponding first, Second and the 3rd the region being spatially separated from light；With the first and second photodetectors, wherein first photodetector use In detection is from each light sent in first and second region and second photodetector is used for detection from institute State second and the 3rd each light sent in region.These specific mode such as Chinese invention patent applications, application number All relevant reading equipment disclosed in 200410045273.4,200410045275.3,200410063910.0, read equipment Detection on detection (test) element (test is chemically examined) result is read, and reads how equipment is fixed on by attachment structure All embodiments on reagent strip, which can be all used in the present invention, is used as a specific embodiment.

A ring-type cell body 131 can also be set in the more excellent specific embodiment of other, on barrier component, The cell body breaks to lower process close to the sample areas of detecting element from tabula, and an elastic sealing ring 13 is set on cell body, when Detection means is inserted into test chamber 14, barrier component test chamber be divided into above and below two independent chambers, elastic sealing ring allows Barrier component is preferably contacted with the inwall 141 of test chamber, so as to reach more preferable segregation.Elastic sealing ring 13 can be Those unwetted materials are constituted, such as rubber, flexible plastic.More excellent, a housing 12 is set on barrier component, and it is wrapped Bottom plate 125 is included, positioned at the side wall 127 of bottom plate the right and left, 128, and lower wall 129, they surround a groove jointly, recessed The element 11 of one absorption liquid is set in groove.Because detecting element is located in test chamber by the through hole 124 on barrier component, Be constantly present some slits 456 in detecting element and through hole, the liquid sample in test chamber be possible to by " accident " and pass through These slits enter in groove, and so setting absorber element in a groove can quickly absorb these liquid samples, can be with Effectively prevent the electronic component on these liquid sample contact arrangements.In addition, after detection is completed, it is believed that test result Need to do further confirmation analysis, such as with the general instrument of liquid phase matter, it is necessary to the liquid sample in detection means and test chamber It is transferred to analysis center together to be analyzed, this when, detection means may be shaken or tilt in transportation, this Sample is located at the liquid sample in test chamber and may entered by opening 120, through hole 124 or slit 456 in groove, in some instances it may even be possible to pass through Electronic component is crossed to be leaked to outside detection means.So, the barrier component of setting can prevent liquid sample from contacting electronic component, together When the absorber element that sets preferably can absorb liquid sample quick after liquid sample enters detection epicoele, prevent liquid Body sample damage electronic component is leaked to the pollution caused outside test chamber to environment.

Detection means can also include liquid sample transfer chamber 15；And one between sample transfer chamber and test chamber Porous membrane 16, when the detection means is inserted into a liquid sample collection chamber 72, the liquid sample in collecting chamber enters In liquid sample transfer chamber, but the liquid sample can not be entered in test chamber 14 by film 16；And, wherein, when a piston 722 are inserted into liquid sample transfer chamber 15, and the piston forces the partially liq sample in transfer chamber to pass through the porous membrane 16 enter in test chamber 14.

As Fig. 1-5 illustrates the specific embodiment of the present invention.Now give and describe in detail.Collecting chamber 72, one end is opened Mouth is used to receiving or housing liquid sample, and a piston 722 is located at the bottom of collecting chamber and upward.Detection means 10 includes one Individual test chamber 14, the test chamber can accommodate detecting element and receive the liquid sample from transfer chamber, and a liquid sample Transfer chamber 15, sets a plastic tab for carrying several apertures, test chamber and air are communicated between transfer chamber and test chamber. Also, set aperture size or shape allow aperture that there is certain surface tension of liquid, for example allow aperture interior diameter be 1.0 Millimeter.Meanwhile, test chamber, with foraminate plastic tab and transfer chamber it is plastics once injection moulding；Collecting chamber and it is located at The piston in bottom of chamber portion is also plastics once injection moulding.When in use, when have collected a certain amount of liquid in collecting chamber After sample, substantial amounts of liquid sample is collected in collecting chamber, and then detection part is inserted into collecting chamber, is being inserted and is being leaned on During nearly piston, the partially liq sample in collecting chamber is entered in transfer chamber 15 by transfer chamber opening 204, in transfer chamber Gas be rejected to by aperture in test chamber.With being further moved downward for transfer chamber, the gas in transfer chamber Body is drained, and liquid sample is almost completely filled with whole transfer chamber, and surface tension is now formd at aperture, even if transfer chamber Interior liquid surface is less than the liquid in the liquid level in collecting chamber, transfer chamber and can not entered by aperture in test chamber.With Transfer chamber is further moved downward, and piston is inserted into transfer chamber, force the liquid sample in transfer chamber to pass through aperture Contacted into test chamber with the sample areas of detecting element.

In addition to concrete mode more than, the aperture film with this property can be selected arbitrarily, for example, select those Film with micro hole, such as cellulose nitrate film, nylon film or filter paper, glass fibre etc..Certainly it can also be tool There is the plastic sheet of micro-aperture.In some preferred embodiments, test chamber 14 and transfer chamber 15 public a faces, example Such as 16 on Fig. 4, the face separates test chamber and transfer chamber, and some apertures are set on the face 16, the connection inspection of these apertures Survey chamber and transfer chamber.In a preferred mode, detection means is once injection moulding, and public isolation face 16 is modeling Tablet, thickness is about 1.5 millimeters, and some apertures are set on plastic sheet.In other examples of implementation, set on film The surface tension of liquid that has of aperture, liquid surface can carry out any internal diameter for setting, for example, changing aperture as needed Size, the perforate depth of aperture, the combination of the shape of aperture or these features.The quantity of aperture can be one or more, It can be 2,3,4,5 or more；The diameter of aperture can be 1-5 millimeters, can be 0.1,0.2,0.3,0.4, 0.5th, 0.8,0.9,1,2,3,4,5 or 6 millimeters；The shape of aperture can be cylindrical or taper or other shapes；Aperture Thickness is 0.1-10 millimeters, can be 0.1,0.2,0.5,1.0,1,3,5,7,9,2,4 or 6 millimeter etc..In a specific implementation In example, aperture is cylindrical, and the interior diameter of aperture is 0.5 millimeter, and depth is 1.5 millimeters, or tapered aperture or Back taper aperture.Change the size of these apertures, the mode of shape or depth there are many kinds, can be by selecting different materials Film, filter paper, glass fibre of micro-aperture etc..The mode that test chamber and transfer chamber are separated also has many kinds, for example first It is his both ends open by once injection moulding hollow circular cylinder, then the position in the middle of cylinder sets one layer of band There is the film of micro hole, such as a layer thickness is 1.5 millimeters of filter paper, and the cylinder is separated into two chambers by the layer film, is examined Survey chamber 15 and transfer chamber 14.

In some cases, due to the continuity and transience of sampling operation, the liquid sample come out from aperture is sometimes Enter in " injection " shape in test chamber.In order to prevent these liquid samples to be ejected into the other elements in test chamber, The top of aperture sets a protection element, and the element can be ejected into other elements with barrier liquid, while not influenceing liquid again Body sample and detecting element contact.For example shown in Fig. 5, one inverted " u "-shaped structure 108 is set in the top of aperture, the structure can Her element contact is contacted without with it with detecting element in test chamber to allow the liquid sample in " injection " shape to enter.Particularly When containing electronic component in test chamber, electronic component more safety can be allowed without because Fluid Contacting using protection element Body sample and destroy some functions.

Specific embodiment of the detailed description present invention is given in conjunction with accompanying drawing 1-10.Fig. 1-5 provides one Detection part, including lid 19 are used for covering in the opening 723 of collecting chamber 721.Include a generally cuboid on lid Test chamber 14 be used for receptive liquid sample and accommodate detecting element.The one end open 142 of test chamber 14 and the other end set one Plastic tab with 4 apertures, the thickness of thin slice is 1.2 millimeters, a diameter of 0.4 millimeter of each aperture.On lid also Including a liquid sample transfer chamber 15, its one end and plastic tab connection, another end opening.Lid, sample transfer chamber, inspection Survey chamber and with foraminate plastic tab by an injection molding.The one end open of collecting chamber, other end sealing is used as bottom Portion 724, one projection of setting, the piston 722 matched with transfer chamber size on bottom.Collecting chamber and piston are connected as a single entity simultaneously By once injection moulding.One detection means 101 is set in test chamber, and detection means includes a He of detecting element 102 The reading equipment of read test result.Detecting element is immunoreagent bar, including receives the He of sample areas 1021 of liquid sample Detection zone 1022；Reading equipment includes the connection member 104 of circuit board 103 and fixed test element, in connection member 104 Side installs circuit board, and detecting element 12 is installed in the lower section corresponding position of connection member 104, while allowing the detection of detecting element Region 1022 is corresponding with the electronic component of the read test result on circuit board, such as light emitting diode and photoelectric detector. One end of connection member stretches out, and forms a horizontal cut-off 109 vertical with connection member, laterally cut-off and connecting portion Part is wide.Laterally further extension forms a hemispheric barrier element 108 to cut-off, can stop from plastics small hole injection The liquid sample gone out.Circuit board 103, test-strips 102, connection member 104 can be assembled as an entirety, as shown in figure 13. The global facility of assembling can be located on a housing 12, and a bottom plate 125, the both sides on bottom plate 125 or so are included on housing One side wall 127 is set respectively, 128, and lower end sets a side wall 129, bottom plate and side wall are encircled a city a groove, are encircled a city simultaneously Groove size and shape matched with test chamber, test chamber can be allowed just to accommodate down whole housing 12, such as the institutes of Fig. 3 and 1 Show.The element 11 of an absorption liquid, such as sponge or filter paper are placed in groove.Meanwhile, one is opened in lower wall 129 and is lacked Mouth 120, can break with the tabula in connection member 104 and cooperatively form tight fit, as shown in Figures 6 and 7.In addition, in lower wall On open a through hole 124, the size matching of its size dimension and reagent strip allows the sample areas of reagent strip to pass through the hole 124 Enter in test chamber and liquid sample contact.An annular groove 131 is also set up in lower wall in addition, one is set on annular groove 1 Individual elastic sealing ring 13.When the detection means housing and on housing be put into test chamber after, sealing ring 13 allows down Side wall 129 closely coordinates with the interior walls 141 of test chamber, and the lower wall and sealing ring on such housing are divided into test chamber Epicoele 143 and detection cavity of resorption 144 are detected, as shown in Fig. 4 and Fig. 3.In order to allow reagent strip position fix and allow circuit board 103, even The position of relay part 104 is fixed, and the structure 122,123 of a pin hole 121 and support fixating reagent bar is set on bottom plate 125. One latch 102 is set so in connection member 104, when assembling, allows latch to be inserted on pin hole, such as Fig. 9 and 10 It is shown, so allow housing and electronic component, reagent strip by connection member be connected as one it is whole, allow on reagent strip and circuit board Positions of electronic parts be relatively fixed, so add the stability of whole detection device.

Detection means 101 with housing is inserted into after test chamber and is structure as a whole with the essence of lid 19, such as Fig. 3 and 4 It is shown.Allow the screen 101 of display result is exposed to facilitate operator to obtain testing result.When in use, lid is covered In the opening of collecting chamber and rotate, the liquid sample in such collecting chamber is flowed into transfer chamber 15, due to being provided with the big of aperture Small, aperture has certain surface tension, even if transfer chamber is less than the liquid level of collecting chamber, the liquid of transfer chamber also will not be by small Hole is entered in test chamber, while the air in transfer chamber is discharged to outside transfer chamber by aperture.It is further with lid Rotation, the opening 204 of transfer chamber, by piston seal, prevents more liquid samples from entering in transfer chamber close to piston.With lid The further rotation of body, piston is inserted into transfer chamber, and the liquid sample in transfer chamber is forced through aperture and enters inspection Survey and contacted in cavity of resorption with the sample areas on reagent strip, liquid sample is because capillarity enters detection zone.Meanwhile, in rotation During, the horizontal cut-off being arranged on detection cavity of resorption can prevent liquid sample from entering and detect that epicoele destruction is located at upper intracavitary Electronic component.Electronic component on the progress of detection zone chemical reaction, circuit board draws test knot by calculating and operation Really, and it is shown on screen.Generally, in some cases, due to the individual difference of operator, lid is covered to collecting chamber simultaneously The speed for rotating lid is different, and the speed that liquid enters test chamber also differs just the same.When with than faster speed swivelling cover When body, due to quick movement of the piston in transfer chamber, so as to cause similar " fountain " shape of liquid to be injected into detection Lower intracavitary.The lower section broken in tabula, the top for facing aperture sets hemispheric stop member 108, similar " fountain " shape liquid Body contacts hemispherical, so as to be reflected into the lower intracavitary of detection by hemispheric inner surface, is contacted with sample areas.

After detection is completed, it may be necessary to be transported further to chemical examination center and carry out confirmation analysis, test is located at detection The liquid sample of cavity of resorption may enter detection epicoele by the opening 124 at test-strips or slit 456, when this unexpected situation After generation, the absorber element in groove, such as sponge, face can quickly absorb liquid sample, it is to avoid liquid sample is revealed To outside test chamber, for example, it is leaked on lid.

In another embodiment, in order to ensure enough this samples of liquid into the lower intracavitary of detection, in circuit board Upper setting a pair of electrodes 107, electrode can enter the lower intracavitary of detection by the opening in lower wall.When there is abundance in test chamber Liquid when, electrode is by fluid connection, and the electronic component on energizing circuit plate is started working, and to the inspection in test-strips Survey region and carry out test result reading and calculating.On the contrary, if the liquid sample in test chamber is inadequate, electrode can not be connected Logical, after one end time is waited, if electrode is again without being connected, system provides the result of a negative, the negative knot Fruit energizing circuit is without any work, and display information on the display screen, the not enough result of indicating liquid.

Detecting element can be some laboratory reagent bars, for example, can be any conventional lateral-flow assays reagent strip, such as Reagent strip disclosed in EP291194 or US6352862.Reagent strip preferably includes porous carrier, and the carrier includes one The particular combination reagent of the not tape label of label particular combination reagent and one.Specifically, EP291194 discloses a kind of immune survey Determine reagent strip, including：The carrier of one porous, the carrier includes a kind of particular combination of the particulate label for assay Reagent, under dampness, the reagent can be moved freely；With a kind of particular combination of the not tape label for same assay Reagent, the reagent is fixed in the not detection zone in the specific adhesive agent downstream of tape label or test section.Suspect containing The liquid sample for stating assay is applied to the carrier of the porous, in the specific of the assay thereon and the particulate label Binding reagents are acted on, and form a kind of complex of assay-combination.The particulate label is coloured, is typically golden or dye Color condensate, for example, latex or poly- sub- peace fat.The complex is subsequently moved within detection zone, in detection zone and the fixation not The particular combination reagent of tape label constitutes another mesocomplex so that the existence range of the assay is detected or observed.

" sample, refer to needing to chemically examine any material that whether there is and (or) analyze analyte concentration, or need Determine one or more samples whether there is and (or) quantity analyte material, or need to carry out qualitative evaluation Material.Sample can be liquid sample, such as liquid sample.Liquid sample includes body fluid, such as blood, serum, blood plasma, saliva Liquid, urine, tears, seminal fluid and marrow；Liquid sample can also be water sample, such as seawater, river, river etc., or come oneself Front yard water, municipal water use or industrial water resource, runoff water or sewage；Sample can be food sample, such as milk and wine.It is viscous Liquid, semisolid or solid sample can be for making liquid, eluate, suspension or leachate equal sampleses.For example, throat or life Grow device sample and may be dipped in and sample is made in liquid.Sample can include liquid, the mixture of solid and gas or any phase Cell suspending liquid in the mixture of pass, such as dilution or solution.Sample includes biological substance, such as cell, microorganism, thin Born of the same parents' device and biochemical complexes.Liquid sample can be from such as soil, excrement, tissue, organ, biological fluid or other natures The solids such as non-liquid samples, semi-solid or high-viscosity material are produced.For example, these solids or semi-solid samples can be with dilutions The appropriate solution mixing of one class.Sample can be macerated and flushed, freezes and thaw, or other extracting methods formation liquid sample.It is surplus Remaining particulate material can use traditional method such as filtering or precipitation to remove.

Analyte can be " drug abuse " (DOA) or other deposit material interested in the sample." drug abuse Thing, (DOA) refers to that non-medical destination uses medicine (generally playing paralysis nerve).Body can be caused by abusing these medicines Body and spirit suffer damage, and produce dependence, addicted and/or death.The example of drug abuse includes cocaine；An Feita Bright (for example, black beauty, white amphetamine tablet, dextroamphetamine, dexie, Beans)；Methylbenzene third Amine (crank, meth, crystal, speed)；Barbiturate (such as Valium, Roche Pharmaceuticals, Nutley, NewJersey)；Sedative (paramedicines of sleeping)；Lysergic acid diethylamide (LSD)；Inhibitor (downers, Goofballs, barbs, blue devils, yellow jackets, methaqualone)；The anti-antidepressant of tricyclic antidepressants (TCA, i.e., third Miaow piperazine, amitriptyline and doxepin)；Hog (PCP)；Tetrahydrocannabinol (THC, pot, dope, hash, weed, Deng.)；Opiate (i.e. morphine, opium, codeine, heroin, hydroxyl dihydrocodeinone)；Anxiolytic and hypnotic sedative agent, Anxiolytic is that a class is mainly used in anxiety reduction, anxiety, fear, is set the mind at rest, and has the medicine of hypnosis sedation concurrently, including Benzodiazepines (benzodiazepines, BZ), atypia BZ classes, fusion phenodiazine NB23C classes, benzene nitrogen tall and erect class, BZ acceptors are matched somebody with somebody Body class, open loop BZ classes, diphenylmethane derivatives, piperazine carboxylic acid salt, piperidine carboxylic acid salt, Kui oxazoline ketone, thiazine and thiazole Derivative, other heterocyclics, imidazole type calmness/anodyne, propanediol derivative-carbamates, aliphatic compound, anthracene class Derivative etc..It can be used for detecting using the device and belong to medical usage but the easily detection of overdose, such as tricyclic antidepressants Antidepressants (imipramine or the like) and Paracetamol etc..These medicines can resolve into different small points after being absorbed by the body Sub- material, these small-molecule substances are present in the body fluid such as blood, urine, saliva, sweat or part body fluid has above-mentioned small point Sub- material.

Claims (14)

1. a kind of detection means, including：

One detecting element, its sample areas and a reception including contact liq sample are from sample areas liquid sample Detection zone；With the reading equipment for being used to read testing result in detection zone, the equipment includes electronic component；And detection Chamber；It is characterized in that, the detection means also obstructs the liquid sample contacted with sample areas including one and entered in test chamber simultaneously Contact the barrier component of electronic component；Test chamber is divided into detection epicoele and detection cavity of resorption by described barrier component, described Sample areas is located at the lower intracavitary of detection, and electronic unit is located at the upper intracavitary of detection；The device also includes a liquid sample transfer chamber, One aperture film is set wherein between transfer chamber and test chamber；Wherein, when the detection means is inserted into a liquid-like In this collecting chamber, the liquid sample in collecting chamber enters in liquid sample transfer chamber, but the liquid sample can not be by described Aperture film enters in test chamber；And, wherein, when a piston is inserted into liquid sample transfer chamber, the piston, which is forced, to be turned The partially liq sample moved in chamber is entered in test chamber by the aperture film.

2. device as claimed in claim 1, it is characterised in that barrier component is located at sample areas and electronics on detecting element Between element.

3. device as claimed in claim 1, it is characterised in that described barrier component also includes one and carrys out self-inspection for absorbing Survey the absorber element of cavity of resorption liquid sample.

4. device as claimed in claim 3, it is characterised in that the device also includes a groove, and it is recessed that absorber element is located at this In groove.

5. device as claimed in claim 4, it is characterised in that described absorber element is located at detection epicoele.

6. device as claimed in claim 5, it is characterised in that described groove is connected as a single entity with barrier component.

7. device as claimed in claim 3, it is characterised in that also include a ring-type cell body on barrier component, in endless groove One is set on body is used for the elastic sealing element of hermetic barrier part and detection luminal wall surface.

8. device as claimed in claim 3, it is characterised in that the detection means also includes a lid, test chamber and lid It is connected as a single entity.

9. device as claimed in claim 3, it is characterised in that described barrier component includes a through hole, detecting element Sample areas is located at detection cavity of resorption by the through hole.

10. device as claimed in claim 1, it is characterised in that described aperture is arranged to surface tension of liquid, when When the pressure differential of the transfer chamber and test chamber is less than or equal to the surface tension of liquid set by the aperture, in transfer chamber Liquid sample test chamber can not be entered by the aperture；When the pressure differential of the transfer chamber and test chamber is more than set by the aperture When the surface tension of liquid put, the liquid in transfer chamber can be entered in test chamber by described aperture.

11. device as claimed in claim 1, it is characterised in that described reading equipment includes：Counting circuit, response is represented The signal of a kind of amount of assay or the accumulative speed of assay, if the signal exceedes default the before chemical examination is not completed One threshold value or less than default Second Threshold, then it represents that result of laboratory test, wherein, described Second Threshold is less than first threshold.

12. device as claimed in claim 1, it is characterised in that described reading equipment includes：At least one light source, its energy It is enough luminous, these light incide at least one in the area that two or more are spatially separated on the detecting element on； At least one photodetector, arrangement are it is positioned such that the light from each area outflow in two areas can be detected, and produce The raw signal represented in respective area presence or absence of liquid sample；And a counting circuit, the signal is responded to count Liquid sample is calculated along the flow velocity flowed between the detecting element Shang Liangge areas；Judge calculated flow velocity whether acceptable pre- Outside definite limitation.

13. device as claimed in claim 1, it is characterised in that described reading equipment includes：First, second, and third light Source, it can be sent for irradiating corresponding first, second, and third region being spatially separated from the detecting element Light；With the first and second photodetectors, wherein first photodetector be used for detect from first and second region Each light sent and second photodetector be used to detect each hair from described second and the 3rd in region The light gone out.

14. device as claimed in claim 1, it is characterised in that also including one in described reading equipment is used to judge The whether enough electrodes of liquid sample.